CN106177942A - A kind of for living paratyphoid vaccine for piglets adjuvant - Google Patents
A kind of for living paratyphoid vaccine for piglets adjuvant Download PDFInfo
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- CN106177942A CN106177942A CN201610684088.2A CN201610684088A CN106177942A CN 106177942 A CN106177942 A CN 106177942A CN 201610684088 A CN201610684088 A CN 201610684088A CN 106177942 A CN106177942 A CN 106177942A
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- adjuvant
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- hydroxide gel
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/025—Enterobacteriales, e.g. Enterobacter
- A61K39/0275—Salmonella
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
- A61K2039/552—Veterinary vaccine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55505—Inorganic adjuvants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55511—Organic adjuvants
- A61K2039/55583—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55588—Adjuvants of undefined constitution
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The invention discloses a kind of for living paratyphoid vaccine for piglets adjuvant, aluminium hydroxide gel, calcium phosphate, vitamin E, lentinan, lanoline are organically combined by the present invention, coordinate mutually, prepare the immunity composite adjuvant for living paratyphoid vaccine for piglets, play the sharpest edges of adjuvant, share immune effect preferably with antigen, toxicity is little, low cost.The side effect of vaccine can be significantly reduced, and improve immune effect.
Description
Technical field
The present invention relates to vaccine adjuvant technical field, particularly relate to a kind of for living paratyphoid vaccine for piglets adjuvant.
Background technology
Baby swine paratyphoid is also referred to as pig salmonellosis, is by a kind of infectious disease of salmonellal piglet.Acute person is for losing
Mass formed by blood stasis, chronic person is necrotic enteritis, often betides 6 monthly ages below piglet, and particularly 2～4 monthly age piglets are common, throughout the year
All can occur, when rainy humidity, cold, season alternation, incidence rate is high.After existing baby swine paratyphoid vaccine injection, some pig is anti-
Should be relatively big, some piglets there will be body temperature and raise, shake, vomit and subtract the symptoms such as food.
Adjuvant is initially to be described as a kind of material with specific antigen use in conjunction by Gaston Ramon
, and with the specific antigen of adjuvant use in conjunction now produced immunne response when being used alone than it much higher.
Adjuvant is nonspecific immunity strengthening agent, when injecting together with antigen or being previously implanted body, and can enhancing body
Immunne response or change type of immune response to antigen.It is the physics by changing antigen that adjuvant strengthens the mechanism of immunne response
Shape, extends antigen retention time in body；Stimulate the mononuclear phagocyte submission ability to antigen；Lymphocyte is stimulated to divide
Change, increase and expand immunne response ability.
Existing immunological adjuvant, because weak effect or virulence are big, especially lacking of property, do not have one to be satisfied with
Effect.Therefore it is an object of the invention to provide a kind of immunologic adjuvant for living paratyphoid vaccine for piglets.
Summary of the invention
The purpose of the present invention is aiming at the defect of above-mentioned existence and provides a kind of for living paratyphoid vaccine for piglets adjuvant,
The present invention by aluminium hydroxide gel, calcium phosphate, vitamin E, lentinan, lanoline organically combine, coordinate mutually, prepare for
The immunity composite adjuvant of living paratyphoid vaccine for piglets, plays the sharpest edges of adjuvant, share immune effect preferably with antigen, toxicity
Little, low cost.The side effect of vaccine can be significantly reduced, and improve immune effect.
The one of the present invention for living paratyphoid vaccine for piglets adjuvant technical scheme is, including aluminium hydroxide gel, calcium phosphate,
Vitamin E, lentinan, lanoline.
Described one is used for living paratyphoid vaccine for piglets adjuvant, the following component including by weight: aluminium hydroxide gel
20-60, calcium phosphate 20-40, vitamin e1 0-20, lentinan 1-10, lanoline 10-30.
Described one is used for living paratyphoid vaccine for piglets adjuvant, the following component including by weight: aluminium hydroxide gel
30-55, calcium phosphate 25-35, vitamin e1 2-16, lentinan 2-8, lanoline 15-20.
Described one is used for living paratyphoid vaccine for piglets adjuvant, the following component including by weight: aluminium hydroxide gel
38, calcium phosphate 30, vitamin e1 5, lentinan 4, lanoline 17.
The preparation method of aluminium hydroxide gel is: by the soda of the Alumen of mass concentration 25%-28% Yu mass concentration 10-12%
Solution, is stirred continuously with 60rpm speed, pH value 6.5-6.8 50 DEG C～60 DEG C mixing in temperature, is passed through steam pipe and carries out ripe
Change, drive away CO2Make colloidal state thinning, burnt hair, blue or green bright for ripening standard, blow the boiling time to there is no a large amount of bubble, pH7.6～
Between 7.8；Adding cold water makes ripening glue temperature drop to less than 75 DEG C, stirs；Washing after draining mother solution, washing water temperature exists
50～55 DEG C, smash glue salt with splash bar during washing, stir, wash 5-6 time, after checking that sulfate is qualified, stop washing；Make
Glue amount moisture content obtains aluminium hydroxide gel when taking off 2～2.5 times to about Alumen inventory.
Described one is used for living paratyphoid vaccine for piglets adjuvant, and preparation method is:
(1) physiological saline solution aluminium hydroxide gel is used；
(2) adding in aluminium hydroxide gel mixed liquor of normal saline by calcium phosphate, vitamin E, lentinan, lanoline, mixing is all
Even, adjust pH to make adjuvant to 6.5.
In step (1), using physiological saline solution aluminium hydroxide gel, aluminium hydroxide gel mass percentage content is 20%.
Antigen is added in adjuvant according to the weight ratio of 1:1, according to 3000rpm, emulsifying 30 minutes.
The invention have the benefit that the present invention is by aluminium hydroxide gel, calcium phosphate, vitamin E, lentinan, lanoline
Organically combining, coordinate mutually, prepare the immunity composite adjuvant for living paratyphoid vaccine for piglets, the maximum playing adjuvant is excellent
Gesture, share immune effect preferably with antigen, and toxicity is little, low cost.The side effect of vaccine can be significantly reduced, and improve immunity effect
Really.
Adjuvant of the present invention is combined with living paratyphoid vaccine for piglets antigen, forms optimal formula, i.e. forms immunostimulation multiple
Compound, preferably plays immunological adjuvant effect.Apply this adjuvant and be used alone aluminium hydroxide gel adjuvant or use existing
Freund adjuvant, it is respectively configured living paratyphoid vaccine for piglets live vaccine immune animal, applies its antibody titer of adjuvant group of the present invention equal
Apparently higher than being used alone adsorbed onto alum adjuvant or using existing Freund adjuvant experimental group, exceed 8-12 antibody titer.
Detailed description of the invention:
In order to be more fully understood that the present invention, describe technical scheme in detail with instantiation below, but the present invention
It is not limited thereto.
Embodiment 1
Described one is used for living paratyphoid vaccine for piglets adjuvant, the following component including by weight: aluminium hydroxide gel 38, phosphorus
Acid calcium 30, vitamin e1 5, lentinan 4, lanoline 17.
The preparation method of aluminium hydroxide gel is: by the soda of the Alumen of mass concentration 25%-28% Yu mass concentration 10-12%
Solution, is stirred continuously with 60rpm speed, pH value 6.5-6.8 50 DEG C～60 DEG C mixing in temperature, is passed through steam pipe and carries out ripe
Change, drive away CO2Make colloidal state thinning, burnt hair, blue or green bright for ripening standard, blow the boiling time to there is no a large amount of bubble, pH7.6～
Between 7.8；Adding cold water makes ripening glue temperature drop to less than 75 DEG C, stirs；Washing after draining mother solution, washing water temperature exists
50～55 DEG C, smash glue salt with splash bar during washing, stir, wash 5-6 time, after checking that sulfate is qualified, stop washing；Make
Glue amount moisture content obtains aluminium hydroxide gel when taking off 2～2.5 times to about Alumen inventory.
Described one is used for living paratyphoid vaccine for piglets adjuvant, and preparation method is:
(1) using physiological saline solution aluminium hydroxide gel, aluminium hydroxide gel mass percentage content is 20%；
(2) adding in aluminium hydroxide gel mixed liquor of normal saline by calcium phosphate, vitamin E, lentinan, lanoline, mixing is all
Even, adjust pH to make adjuvant to 6.5.
Antigen is added in adjuvant according to the weight ratio of 1:1, according to 3000rpm, emulsifying 30 minutes.
According to national basic veterinary drug standard, verify its result:
Safety verification: with more than 30 ages in days, body weight 12kg, delactational piglets 20, after every ear, superficial muscular vaccinates 1ml.
Observe 24 hours, all should have no adverse reaction.After observing 10, all there is not untoward reaction in 20 piglets.
Efficacy test: immune group with more than 30 ages in days, body weight 12kg, delactational piglets 10, superficial muscular note after every ear
Penetrating vaccine 1ml, after 30 days, together with the non-immunized controls piglet 5 that condition is identical, intravenous injection digests through meat liver stomach (film)
Salmonella choleraesuls (CVCC79102 strain) bacterium solution 2.0ml (containing 1MLD viable bacteria) in 2 generations cultivated by soup.Isolation is raised
Support, observe 21, the survival condition of record piglet.
Assay: comparison piglet falls ill entirely, and immune group protective rate is 100%.
Embodiment 2
Described one is used for living paratyphoid vaccine for piglets adjuvant, the following component including by weight: aluminium hydroxide gel 42, phosphorus
Acid calcium 28, vitamin e1 2, lentinan 5, lanoline 15.
The preparation method of aluminium hydroxide gel is: by the soda of the Alumen of mass concentration 25%-28% Yu mass concentration 10-12%
Solution, is stirred continuously with 60rpm speed, pH value 6.5-6.8 50 DEG C～60 DEG C mixing in temperature, is passed through steam pipe and carries out ripe
Change, drive away CO2Make colloidal state thinning, burnt hair, blue or green bright for ripening standard, blow the boiling time to there is no a large amount of bubble, pH7.6～
Between 7.8；Adding cold water makes ripening glue temperature drop to less than 75 DEG C, stirs；Washing after draining mother solution, washing water temperature exists
50～55 DEG C, smash glue salt with splash bar during washing, stir, wash 5-6 time, after checking that sulfate is qualified, stop washing；Make
Glue amount moisture content obtains aluminium hydroxide gel when taking off 2～2.5 times to about Alumen inventory.
Described one is used for living paratyphoid vaccine for piglets adjuvant, and preparation method is:
(1) using physiological saline solution aluminium hydroxide gel, aluminium hydroxide gel mass percentage content is 20%；
(2) adding in aluminium hydroxide gel mixed liquor of normal saline by calcium phosphate, vitamin E, lentinan, lanoline, mixing is all
Even, adjust pH to make adjuvant to 6.5.
Antigen is added in adjuvant according to the weight ratio of 1:1, according to 3000rpm, emulsifying 30 minutes.
According to national basic veterinary drug standard, verify its result:
Safety verification: with more than 30 ages in days, body weight 12kg, delactational piglets 20, after every ear, superficial muscular vaccinates 1ml.
Observe 24 hours, all should have no adverse reaction.After observing 10, all there is not untoward reaction in 20 piglets.
Efficacy test: immune group with more than 30 ages in days, body weight 12kg, delactational piglets 10, superficial muscular note after every ear
Penetrating vaccine 1ml, after 30 days, together with the non-immunized controls piglet 5 that condition is identical, intravenous injection digests through meat liver stomach (film)
Salmonella choleraesuls (CVCC79102 strain) bacterium solution 2.0ml (containing 1MLD viable bacteria) in 2 generations cultivated by soup.Isolation is raised
Support, observe 21, the survival condition of record piglet.
Assay: comparison piglet falls ill entirely, and immune group protective rate is 100%.
Claims (8)
1. one kind be used for living paratyphoid vaccine for piglets adjuvant, it is characterised in that include aluminium hydroxide gel, calcium phosphate, vitamin E,
Lentinan, lanoline.
One the most according to claim 1 is used for living paratyphoid vaccine for piglets adjuvant, it is characterised in that include by weight
Following component: aluminium hydroxide gel 20-60, calcium phosphate 20-40, vitamin e1 0-20, lentinan 1-10, lanoline 10-30.
One the most according to claim 1 is used for living paratyphoid vaccine for piglets adjuvant, it is characterised in that include by weight
Following component: aluminium hydroxide gel 30-55, calcium phosphate 25-35, vitamin e1 2-16, lentinan 2-8, lanoline 15-20.
One the most according to claim 1 is used for living paratyphoid vaccine for piglets adjuvant, it is characterised in that include by weight
Following component: aluminium hydroxide gel 38, calcium phosphate 30, vitamin e1 5, lentinan 4, lanoline 17.
One the most according to claim 1 is used for living paratyphoid vaccine for piglets adjuvant, it is characterised in that aluminium hydroxide gel
Preparation method is: by the soda ash solution of the Alumen of mass concentration 25%-28% and mass concentration 10-12% in temperature at 50 DEG C～60
DEG C mixing, is stirred continuously with 60rpm speed, pH value 6.5-6.8, is passed through steam pipe and carries out ripening, drive away CO2Make colloidal state thinning,
Burnt hair, blue or green bright for ripening standard, blow the boiling time to there is no a large amount of bubble, between pH7.6～7.8；Add cold water and make ripening glue
Temperature drops to less than 75 DEG C, stirs；Wash after draining mother solution, washing water temperature at 50～55 DEG C, splash bar during washing
Smash glue salt, stir, wash 5-6 time, after checking that sulfate is qualified, stop washing；Glue amount moisture content is made to take off to about Alumen
Aluminium hydroxide gel is obtained during 2～2.5 times of inventory.
One the most according to claim 1 is used for living paratyphoid vaccine for piglets adjuvant, it is characterised in that preparation method is:
(1) physiological saline solution aluminium hydroxide gel is used；
(2) adding in aluminium hydroxide gel mixed liquor of normal saline by calcium phosphate, vitamin E, lentinan, lanoline, mixing is all
Even, adjust pH to make adjuvant to 6.5.
One the most according to claim 6 is used for living paratyphoid vaccine for piglets adjuvant, it is characterised in that in step (1), uses
Physiological saline solution aluminium hydroxide gel, aluminium hydroxide gel mass percentage content is 20%.
One the most according to claim 6 is used for living paratyphoid vaccine for piglets adjuvant, it is characterised in that by antigen according to 1:
The weight ratio of 1 adds in adjuvant, according to 3000rpm, emulsifying 30 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610684088.2A CN106177942A (en) | 2016-08-18 | 2016-08-18 | A kind of for living paratyphoid vaccine for piglets adjuvant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610684088.2A CN106177942A (en) | 2016-08-18 | 2016-08-18 | A kind of for living paratyphoid vaccine for piglets adjuvant |
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| CN106177942A true CN106177942A (en) | 2016-12-07 |
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| CN201610684088.2A Pending CN106177942A (en) | 2016-08-18 | 2016-08-18 | A kind of for living paratyphoid vaccine for piglets adjuvant |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109908338A (en) * | 2019-02-26 | 2019-06-21 | 苏州博特龙免疫技术有限公司 | Novel oiliness immunologic adjuvant and preparation method thereof and the application in Antibody preparation |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102245201A (en) * | 2008-10-12 | 2011-11-16 | 麻省理工学院 | Adjuvant incorporation in immunonanotherapeutics |
| CN102647977A (en) * | 2009-10-08 | 2012-08-22 | 阿祖瑞博有限公司 | Formulation of drugs and vaccines in the form of percutaneous injectable needles |
| CN102988982A (en) * | 2012-12-14 | 2013-03-27 | 山东滨州沃华生物工程有限公司 | Method for preparing nano aluminum hydroxide gel adjuvant |
| CN104013955A (en) * | 2014-06-18 | 2014-09-03 | 中国科学院过程工程研究所 | Oil-in-water emulsion free of surfactant and use thereof |
-
2016
- 2016-08-18 CN CN201610684088.2A patent/CN106177942A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102245201A (en) * | 2008-10-12 | 2011-11-16 | 麻省理工学院 | Adjuvant incorporation in immunonanotherapeutics |
| CN102647977A (en) * | 2009-10-08 | 2012-08-22 | 阿祖瑞博有限公司 | Formulation of drugs and vaccines in the form of percutaneous injectable needles |
| CN102988982A (en) * | 2012-12-14 | 2013-03-27 | 山东滨州沃华生物工程有限公司 | Method for preparing nano aluminum hydroxide gel adjuvant |
| CN104013955A (en) * | 2014-06-18 | 2014-09-03 | 中国科学院过程工程研究所 | Oil-in-water emulsion free of surfactant and use thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王永芬等: "《动物生物制品技术》", 31 August 2011 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109908338A (en) * | 2019-02-26 | 2019-06-21 | 苏州博特龙免疫技术有限公司 | Novel oiliness immunologic adjuvant and preparation method thereof and the application in Antibody preparation |
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Application publication date: 20161207 |