CN106172263A - A kind of semi-automatic human excrement schistosoma japonice ovum hatching apparatus and hatching method thereof - Google Patents
A kind of semi-automatic human excrement schistosoma japonice ovum hatching apparatus and hatching method thereof Download PDFInfo
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- CN106172263A CN106172263A CN201610767124.1A CN201610767124A CN106172263A CN 106172263 A CN106172263 A CN 106172263A CN 201610767124 A CN201610767124 A CN 201610767124A CN 106172263 A CN106172263 A CN 106172263A
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Abstract
A kind of semi-automatic human excrement schistosoma japonice ovum hatching apparatus and hatching method thereof, belong to prevention and cure of snail fever technical field.This device includes thick slag filtering module and hatching module, and described thick slag filtering module is connected with hatching module.Its hatching method carries out operating into water for being first positioned over by human excrement in thick slag filtering module, then hatches in hatching module, finally collects sample effluent, it is carried out microexamination.The device using the present invention to provide can quickly draw testing result, reduces reviewer and sample touch opportunity, additionally it is possible to reduces water consumption, reduce labor intensity, possesses the feature that assay is stable.
Description
Technical field
The present invention relates to a kind of semi-automatic human excrement schistosoma japonice ovum hatching apparatus and hatching method thereof, belong to schistosomicide
Sick Prevention Technique field.
Background technology
Schistosomiasis japanica is the serious harm people's health of a kind of Zoonosis and affects livestock economy development
Great infectious disease.The goldstandard of this disease diagnosis mainly finds japonice ovum from patient's feces or stool for hatching is hemorrhage
Trematodiasis miracidium.Conventional push bench process operating difficulties, pollutes operator, uses rayon balls (cotton layer) to filter excrement slag, and it is simultaneously
Block penetrating of miracidium, cause missing inspection to fail to pinpoint a disease in diagnosis, affect the diagnosis and treatment of schistosomicide.
Therefore, being badly in need of developing the penetrating system of system, filtration system and miracidium that automatically blends, a kind of semi-automatic people is invented in research
Excrement schistosoma japonice ovum hatching apparatus, for the etiological diagnosis of people's schistosomicide.The detection of people's schistosomicide can be improved
Rate, also can improve the accuracy of diagnosis.
Summary of the invention
It is an object of the invention to provide a kind of semi-automatic human excrement schistosoma japonice ovum hatching apparatus and hatching method thereof, can
By the human excrement containing japonice ovum through stirring, filter, be enriched with, hatching, reach the purpose of schistosomicide etiological diagnosis.
Technical scheme, a kind of semi-automatic human excrement schistosoma japonice ovum hatching apparatus, filters mould including thick slag
Block and hatching module, described thick slag filtering module is connected with hatching module;
Described thick slag filtering module includes that thick slag filters bottle, upper cover, water inlet, the first valve, stirring paddle and the first screen cloth;Institute
Stating thick slag filtration bottle and be provided with upper cover, above cover and be provided with water inlet, the first valve is arranged on water inlet, and stirring paddle is by upper
Lid is arranged in thick slag filtering flask body;Described first screen cloth is arranged at thick slag and filters bottle body bottom;
Described hatching module includes hatching bottle, the second filter screen, the 3rd filter screen, waste liquid port, the second valve and the 3rd valve;Described
Hatching bottle top is provided with the 3rd valve, and top is provided with the second filter screen, and bottom is provided with the 3rd filter screen, and bottom is provided with waste liquid port, the
Two valves are arranged in waste liquid port, and the 3rd valve is positioned at the second filter screen upper end;
Described thick slag filters bottle body bottom with hatching bottle by pipeline communication, and pipeline leads to one end of hatching module and is positioned at the second filter
Between net and the 3rd filter screen;
Described hatching bottle top is provided with some graduation marks.
The stirring-head of described stirring paddle is two cylinders in decussation, and stir speed (S.S.) is 100-500rpm.
Described filter screen the first screen cloth is 40 mesh, and the second filter screen is 150 mesh, and the 3rd filter screen is 260 mesh.
The scale of described graduation mark is 5,10,15 and 20mL.
The hatching method of described semi-automatic human excrement schistosoma japonice ovum hatching apparatus, step is:
(1) thick slag filtering module water inlet: open upper cover, first the human faecal mass containing schistosome ovum is inserted thick slag filtering module,
Cover upper cover, open stirring paddle, open the first valve on water inlet and start to filter bottle water inlet to thick slag, form liquid dung mixing
Thing, filtrate enters hatching module from connecting line;
(2) hatching module water inlet: filtrate passes through the 3rd filter screen after entering hatching module, opens the second valve so that filtrate is continued
Continuous from waste liquid port outflow, until the liquid dung mixture in thick slag filtering module becomes clarification, close the second valve, the 3rd valve successively
And stirring paddle;Water inlet continues water inlet, until the water level in hatching module reaches assigned scale line, closes water inlet;
(3) hatching: hatching module is carried out illumination, and egg hatch is miracidium, miracidium enters in hatching module through the second filter screen
Portion, after hatching, the 3rd valve opened on sample flow export makes sample flow out, and examines outflow sample under the microscope
Survey.
Beneficial effects of the present invention: the present invention utilizes pulper to pulverize human excrement, improves water inlet filtration system and miracidium is penetrating
System, a kind of semi-automatic human excrement schistosoma japonice ovum hatching apparatus is invented in research, for the etiological diagnosis of schistosomicide.Its
Both improve the recall rate of people's schistosomicide, also improved the accuracy of diagnosis, and be also beneficial to treat schistosomicide patient, control
The propagation of schistosomicide is with popular.
The device using the present invention to provide can quickly draw testing result, reduces reviewer and sample touch opportunity,
Water consumption can also be reduced, reduce labor intensity, possess the feature that assay is stable.
Accompanying drawing explanation
Fig. 1 is present configuration schematic diagram.
Description of reference numerals: 1, thick slag filtering module;1-1, thick slag filter bottle;1-2, upper cover;1-3, water inlet;1-4、
First valve;1-5, stirring paddle;1-6, the first screen cloth;2, hatching module;2-1, hatching bottle;2-2, the second filter screen;2-3,
Three filter screens;2-4, waste liquid port;2-5, the second valve;2-6, the 3rd valve.
Detailed description of the invention
Embodiment 1
As it is shown in figure 1, a kind of semi-automatic human excrement schistosoma japonice ovum hatching apparatus, including thick slag filtering module 1 and hatching mould
Block 2, described thick slag filtering module 1 is connected with hatching module 2;
Described thick slag filtering module 1 includes that thick slag filters bottle 1-1, upper cover 1-2, water inlet 1-3, first valve 1-4, stirring paddle
1-5 and the first screen cloth 1-6;Described thick slag filters bottle 1-1 and is provided with upper cover 1-2, upper cover 1-2 and is provided with water inlet 1-3, and first
Valve 1-4 is arranged on water inlet 1-3, and stirring paddle 1-5 is arranged at thick slag by upper cover 1-2 and filters in bottle 1-1;Described first
Screen cloth 1-6 is arranged at bottom bottle 1-1;
Described hatching module 2 includes hatching bottle 2-1, the second filter screen 2-2, the 3rd filter screen 2-3, waste liquid port 2-4, the second valve 2-
5 and the 3rd valve 2-6;Described hatching bottle 2-1 top is provided with the 3rd valve 2-6, and top is provided with the second filter screen 2-2, and bottom sets
Having the 3rd filter screen 2-3, bottom is provided with waste liquid port 2-4, and the second valve 2-5 is arranged in waste liquid port 2-4, and the 3rd valve 2-6 is positioned at
Second filter screen 2-2 upper end;
Described thick slag filters and passes through pipeline communication with hatching bottle 2-1 bottom bottle 1-1, and pipeline leads to the position, one end of hatching module 2
Between the second filter screen 2-2 and the 3rd filter screen 2-3;
Described hatching bottle 2-1 top is provided with some graduation marks.
The stirring-head of described stirring paddle 1-5 is two cylinders in decussation, and stir speed (S.S.) is 100-500rpm.
Described filter screen the first screen cloth 1-6 is 40 mesh, and the second filter screen 2-2 is 150 mesh, and the 3rd filter screen 2-3 is 260 mesh.
The scale of described graduation mark is 5,10,15 and 20mL.
Embodiment 2
The hatching method of described semi-automatic human excrement schistosoma japonice ovum hatching apparatus, step is:
(1) thick slag filtering module water inlet: open upper cover 1-2, first the human faecal mass containing schistosome ovum is inserted thick slag and filters mould
Block 1, covers upper cover 1-2, opens stirring paddle 1-5, opens the first valve 1-4 on water inlet 1-3 and starts to filter bottle to thick slag
1-1 is intake, and forms liquid dung mixture, and filtrate enters hatching module 2 from connecting line;
(2) hatching module water inlet: filtrate passes through the 3rd filter screen 2-3 after entering hatching module 2, opens the second valve 2-5 so that
Filtrate continues to flow out from waste liquid port 2-4, until the liquid dung mixture in thick slag filtering module 1 becomes clarification, closes the second valve successively
Door 2-5, the 3rd valve 2-6 and stirring paddle 1-5;Water inlet 1-3 continues water inlet, until the water level in hatching module 2 reaches to specify
Scale, closes water inlet 1-3;
(3) hatching: hatching module 2 is carried out illumination, and egg hatch is miracidium, miracidium enters hatching mould through the second filter screen 2-2
Block 2 top, after hatching, the 3rd valve 2-6 opened on sample flow export makes sample flow out, under the microscope to flowing out sample
Product detect.
Application Example 1
Collect Lower Endemic Areas schistosomicide human faecal mass, carry out schistosomicide with original excrement inspection hatching method and the inventive method
Former learning is detected, and it is high that the result display present invention detects positive rate, and method is simple to operate, pollution-free, reliable results.
Table 1 distinct methods schistosomicide testing result
。
Claims (5)
1. a semi-automatic human excrement schistosoma japonice ovum hatching apparatus, it is characterised in that: include thick slag filtering module (1) and incubate
Changing module (2), described thick slag filtering module (1) is connected with hatching module (2);
Described thick slag filtering module (1) includes that thick slag filters bottle (1-1), upper cover (1-2), water inlet (1-3), the first valve
(1-4), stirring paddle (1-5) and the first screen cloth (1-6);Described thick slag filters bottle (1-1) and is provided with upper cover (1-2), upper cover (1-
2) being provided with water inlet (1-3), the first valve (1-4) is arranged on water inlet (1-3), and stirring paddle (1-5) passes through upper cover (1-2)
It is arranged at thick slag to filter in bottle (1-1);Described first screen cloth (1-6) is arranged at thick slag and filters bottle (1-1) bottom;
Described hatching module (2) include hatch bottle (2-1), the second filter screen (2-2), the 3rd filter screen (2-3), waste liquid port (2-4),
Second valve (2-5) and the 3rd valve (2-6);Described hatching bottle (2-1) top is provided with the 3rd valve (2-6), and top is provided with
Second filter screen (2-2), bottom is provided with the 3rd filter screen (2-3), and bottom is provided with waste liquid port (2-4), and the second valve (2-5) is arranged at useless
On liquid mouth (2-4), the 3rd valve (2-6) is positioned at the second filter screen (2-2) upper end;
Described thick slag filters bottle (1-1) bottom with hatching bottle (2-1) by pipeline communication, pipeline logical hatching module (2)
One end is positioned between the second filter screen (2-2) and the 3rd filter screen (2-3);
Described hatching bottle (2-1) top is provided with some graduation marks.
The most semi-automatic human excrement schistosoma japonice ovum hatching apparatus, it is characterised in that: described stirring paddle
(1-5) stirring-head is two cylinders in decussation, and stir speed (S.S.) is 100-500rpm.
The most semi-automatic human excrement schistosoma japonice ovum hatching apparatus, it is characterised in that: described first sieve
Net (1-6) is 40 mesh, and the second filter screen (2-2) is 150 mesh, and the 3rd filter screen (2-3) is 260 mesh.
The most semi-automatic human excrement schistosoma japonice ovum hatching apparatus, it is characterised in that: described graduation mark
Scale be 5,10,15 and 20mL.
5. the hatching method of semi-automatic human excrement schistosoma japonice ovum hatching apparatus described in claim 1, it is characterised in that step
For:
(1) thick slag filtering module water inlet: open upper cover (1-2), first the human faecal mass containing schistosome ovum is inserted thick slag and filters
Module (1), covers upper cover (1-2), opens stirring paddle (1-5), open the first valve (1-4) on water inlet (1-3) start to
Thick slag filters bottle (1-1) water inlet, forms liquid dung mixture, and filtrate enters hatching module (2) from connecting pipeline;
(2) hatching module water inlet: filtrate enters hatching module (2) afterwards by the 3rd filter screen (2-3), opens the second valve (2-
5) so that filtrate continues to flow out from waste liquid port (2-4), until the liquid dung mixture in thick slag filtering module (1) becomes clarification, successively
Close the second valve (2-5), the 3rd valve (2-6) and stirring paddle (1-5);Water inlet (1-3) continues water inlet, until hatching module
(2) water level in reaches assigned scale line, closes water inlet (1-3);
(3) hatching: hatching module (2) is carried out illumination, and egg hatch is miracidium, miracidium enters through the second filter screen (2-2) and incubates
Changing module (2) top, after hatching, the 3rd valve (2-6) opened on sample flow export makes sample flow out, under the microscope
Detect flowing out sample.
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