CN106119299A - A kind of method utilizing vine to prepare ethanol - Google Patents
A kind of method utilizing vine to prepare ethanol Download PDFInfo
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- CN106119299A CN106119299A CN201610544913.9A CN201610544913A CN106119299A CN 106119299 A CN106119299 A CN 106119299A CN 201610544913 A CN201610544913 A CN 201610544913A CN 106119299 A CN106119299 A CN 106119299A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
The invention discloses a kind of method utilizing vine to prepare ethanol, belong to biomass energy technology field, described ethanol is dried through vine, pulverizes, pretreatment, hydrolyze, the step such as fermentation prepares.The present invention can avoid the uncertainty of microbial enzyme fermentation sugar refining technology, reduces technical risk；Sugar conversion ratio of the present invention is high, and the level of residue of generation is few, is environmental type technique；Preparation technology of the present invention is simple, less investment, is suitable for large-scale promotion and application.
Description
Technical field
The invention belongs to biomass energy technology field, be specifically related to a kind of method utilizing vine to prepare ethanol.
Background technology
Along with the growth of population, grain, energy and environment are the main challenges of facing mankind.Produce with cellulosic material
Renewable and clean energy resource is increasingly subject to extensive concern, and is considered as to have most with agricultural waste material for raw material production alcohol fuel
The industry of development prospect.Fructus Vitis viniferae is very wide in China's cultivated area, also produces substantial amounts of vine every year while results fruit.
In vine, protein content is low but carbohydrate content high, and individual plants carbohydrate content is up to the 82% of dry,
Thus be the quality raw materials producing biomass energy.Research to tendril transforming fuel alcohol both at home and abroad starts to walk, but and corn stalk
Stalk is compared as raw material, and current alcohol yied also ratio is relatively low, and it is the most undesirable that bottleneck is that tendril is degraded into the technology of fermentable sugars.Cause
This, the comprehensive exploitation of tendril and the recovery energy of lignocellulose are respectively provided with high by the efficient degradation technique of invention tendril
Economic worth and social benefit.
Utilize lignocellulosic material to produce bio-ethanol has important meaning (biological to alleviating energy crisis and ecological deterioration
Matter Chemical Engineering, 2012,46 (3), 39-44).But during enzyme hydrolysis, due to portion of cellulose enzyme and hemicellulase not
Reversible adsorbs the loss that can cause enzymatic activity on substrate particularly lignin surface, cellulose in addition in the way of ineffective adsorption
The crystalline texture of itself and the complexity of hemicellulose structure are also the key factors causing hydrolysis efficiency low.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of method utilizing vine to prepare ethanol, to solve vine
It is degraded into fermentable sugars and not exclusively causes the problems such as alcohol getting rate is low.The present invention can avoid microbial enzyme fermentation sugar refining technology not
Definitiveness, reduces technical risk；Sugar conversion ratio of the present invention is high, and the level of residue of generation is few, is environmental type technique；The present invention
Hydrolysis temperature relatively low, energy resource consumption is few, beneficially the promotion and application of correlation technique.
In order to solve above technical problem, the present invention by the following technical solutions:
A kind of method utilizing vine to prepare ethanol, comprises the following steps:
S1: use saline solution rinsing vine 2-3h, after rinsing, vine is placed at temperature is 98-105 DEG C drying to aqueous
Amount≤10%, prepares dry vine；
S2: dry vine step S1 prepared is pulverized, and sifts out, and prepares vine granule；
S3: put in microwave reactor by the vine granule that step S2 prepares, adds solution A mix homogeneously, is heated to 65-68
DEG C, pretreatment 0.5-0.8h under speed of agitator is 200-300r/min, filters after being then cooled to room temperature, prepares pretreatment
Vine granule, the natural polymers such as the cellulose of vine, lignin can mutually be dissociated by pretreatment, is conducive to next step
Hydrolysis；Described solution A is the mixed liquor of butanone, ethanol, ammonium phosphate salt, and the mass concentration of described butanone is 8%-10%, described second
The mass concentration of alcohol is 60%-80%, and the mass concentration of described ammonium phosphate salt is 6%-8%, described butanone solution, ethanol solution, phosphorus
The volume ratio of acid ammonium salt solution is 10-20:12-25:1-2, and described vine granule is 1:10-15g/ with the solid-liquid ratio of solution A
mL；Ammonium phosphate salt has potentiation with butanone, the mixed separation making lignin of ethanol；
S4: the vine granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mix homogeneously,
Regulation pH is 1.6-1.8, is heated to 75-78 DEG C, processes 1-1.5h, be then cooled under speed of agitator is 300-400r/min
Filter after room temperature, prepare sugar liquid；Described solution B is the mixed liquor of carbon back sodium sulfonate, sulphuric acid, and the quality of described carbon back sodium sulfonate is dense
Degree is 5%-7%, and the mass concentration of described sulphuric acid is 4%-6%, and described carbon back sodium sulfonate solution, the volume ratio of sulfuric acid solution are 6-
10:5-8, the vine granule of described pretreatment and the solid-liquid ratio of solution B are 1:6-8g/mL；
S5: sugar liquid step S4 prepared accesses yeast, adds carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate,
Controlling temperature is 26-28 DEG C of bottom fermentation, prepares ethanol through dehydration；Described yeast quality is the 9%-11% of sugar liquid quality, described
Carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality are respectively 0.18%-0.22%, 0.02%-of sugar liquid quality
0.04%、0.16%-0.21%、0.08%-0.12%、0.14%-0.16%。
Further, the concentration of saline solution described in step S1 is 10%-15%.
Further, the mesh number of sieve described in step S2 is 120-200.
Further, the temperature of pretreatment in microwave reactor of vine granule described in step S3 is 68 DEG C, rotating speed
For 300r/min, the time is 0.5h.
Further, vine granule described in step S3 is 1:12g/mL with the solid-liquid ratio of solution A.
Further, the vine granule of pretreatment described in step S4 is 1.6 at the pH that ultrasound reactor processes, temperature
Degree is 78 DEG C, and rotating speed is 400r/min, and the time is 1h.
Further, the vine granule of pretreatment described in step S4 and the solid-liquid ratio of solution B are 1:7g/mL.
Further, yeast quality described in step S5 is the 10% of sugar liquid quality, described carbamide, Semen phaseoli radiati powder, di(2-ethylhexyl)phosphate
Hydrogen sodium, magnesium nitrate, calcium sulfate quality are respectively the 0.2% of sugar liquid quality, 0.03%, 0.2%, 0.1%, 0.15%.
The method have the advantages that
(1) present invention prepares ethanol low cost, and raw material sources is wide；
(2) preparation technology of the present invention is simple, less investment, is suitable for large-scale promotion and application；
(3) uncertainty of microbial enzyme fermentation sugar refining technology can be avoided, reduce technical risk；
(4) sugar conversion ratio is high, and the level of residue of generation is few, is environmental type technique；
(5) hydrolysis temperature is relatively low, and energy resource consumption is few, beneficially the promotion and application of correlation technique；
(6) the sugared yield that this method prepares is 92.31%-94.01%, and alcohol getting rate is 85.11%-87.05%, purity of alcohol
For 99.59%-99.71%, it is seen that the method using the present invention can high-yield ethanol.
Detailed description of the invention
For ease of being more fully understood that the present invention, being illustrated by following example, these embodiments belong to the present invention's
Protection domain, but it is not intended to protection scope of the present invention.
In an embodiment, the described method utilizing vine to prepare ethanol, comprise the following steps:
S1: the saline solution rinsing vine 2-3h using concentration to be 10%-15%, it is 98-105 that vine is placed on after rinsing temperature
Dry at DEG C to water content≤10%, prepare dry vine；
S2: dry vine step S1 prepared is pulverized, and crosses 120-200 mesh sieve, prepares vine granule；
S3: put in microwave reactor by the vine granule that step S2 prepares, adds solution A mix homogeneously, is heated to 65-68
DEG C, pretreatment 0.5-0.8h under speed of agitator is 200-300r/min, filters after being then cooled to room temperature, prepares pretreatment
Vine granule；Described solution A is the mixed liquor of butanone, ethanol, ammonium phosphate salt, and the mass concentration of described butanone is 8%-10%,
The mass concentration of described ethanol is 60%-80%, and the mass concentration of described ammonium phosphate salt is 6%-8%, and described butanone solution, ethanol are molten
Liquid, the volume ratio of ammonium phosphate salt solution are 10-20:12-25:1-2, and described vine granule is 1:10-with the solid-liquid ratio of solution A
15g/mL；
S4: the vine granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mix homogeneously,
Regulation pH is 1.6-1.8, is heated to 75-78 DEG C, processes 1-1.5h, be then cooled under speed of agitator is 300-400r/min
Filter after room temperature, prepare sugar liquid；Described solution B is the mixed liquor of carbon back sodium sulfonate, sulphuric acid, and the quality of described carbon back sodium sulfonate is dense
Degree is 5%-7%, and the mass concentration of described sulphuric acid is 4%-6%, and described carbon back sodium sulfonate solution, the volume ratio of sulfuric acid solution are 6-
10:5-8, the vine granule of described pretreatment and the solid-liquid ratio of solution B are 1:6-8g/mL；
S5: sugar liquid step S4 prepared accesses yeast, adds carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate,
Controlling temperature is 26-28 DEG C of bottom fermentation, prepares ethanol through dehydration；Described yeast quality is the 9%-11% of sugar liquid quality, described
Carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality are respectively 0.18%-0.22%, 0.02%-of sugar liquid quality
0.04%、0.16%-0.21%、0.08%-0.12%、0.14%-0.16%。
Below by more specifically embodiment, the present invention will be described.
Embodiment 1
A kind of method utilizing vine to prepare ethanol, comprises the following steps:
S1: the saline solution rinsing vine 2.5h using concentration to be 12%, after rinsing, vine is placed at temperature is 98-105 DEG C
Dry to water content≤10%, prepare dry vine；
S2: dry vine step S1 prepared is pulverized, and crosses 150 mesh sieve, prepares vine granule；
S3: put in microwave reactor by the vine granule that step S2 prepares, adds solution A mix homogeneously, is heated to 66 DEG C,
Pretreatment 0.7h under speed of agitator is 200r/min, filters after being then cooled to room temperature, prepares the vine granule of pretreatment；
Described solution A is the mixed liquor of butanone, ethanol, ammonium phosphate salt, and the mass concentration of described butanone is 9%, and the quality of described ethanol is dense
Degree is 70%, and the mass concentration of described ammonium phosphate salt is 7%, described butanone solution, ethanol solution, the volume ratio of ammonium phosphate salt solution
For 15:18:1, described vine granule is 1:12g/mL with the solid-liquid ratio of solution A；
S4: the vine granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mix homogeneously,
Regulation pH is 1.7, is heated to 76 DEG C, processes 1.2h under speed of agitator is 300r/min, filters, system after being then cooled to room temperature
Obtain sugar liquid；Described solution B is the mixed liquor of carbon back sodium sulfonate, sulphuric acid, and the mass concentration of described carbon back sodium sulfonate is 6%, described sulfur
The mass concentration of acid is 5%, and described carbon back sodium sulfonate solution, the volume ratio of sulfuric acid solution are 8:6, the vine of described pretreatment
Granule is 1:7g/mL with the solid-liquid ratio of solution B；
S5: sugar liquid step S4 prepared accesses yeast, adds carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate,
Controlling temperature is 27 DEG C of bottom fermentations, prepares ethanol through dehydration；Described yeast quality is the 10% of sugar liquid quality, described carbamide, green
Semen Glycines powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality are respectively the 0.2% of sugar liquid quality, 0.03%, 0.2%, 0.1%, 0.15%.
Embodiment 2
A kind of method utilizing vine to prepare ethanol, comprises the following steps:
S1: the saline solution rinsing vine 3h using concentration to be 10%, after rinsing, vine is placed at temperature is 98-105 DEG C baking
Do to water content≤10%, prepare dry vine；
S2: dry vine step S1 prepared is pulverized, and crosses 120 mesh sieve, prepares vine granule；
S3: put in microwave reactor by the vine granule that step S2 prepares, adds solution A mix homogeneously, is heated to 65 DEG C,
Pretreatment 0.8h under speed of agitator is 200r/min, filters after being then cooled to room temperature, prepares the vine granule of pretreatment；
Described solution A is the mixed liquor of butanone, ethanol, ammonium phosphate salt, and the mass concentration of described butanone is 8%, and the quality of described ethanol is dense
Degree is 60%, and the mass concentration of described ammonium phosphate salt is 6%, described butanone solution, ethanol solution, the volume ratio of ammonium phosphate salt solution
For 10:12:1, described vine granule is 1:10g/mL with the solid-liquid ratio of solution A；
S4: the vine granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mix homogeneously,
Regulation pH is 1.6, is heated to 75 DEG C, processes 1.5h under speed of agitator is 300r/min, filters, system after being then cooled to room temperature
Obtain sugar liquid；Described solution B is the mixed liquor of carbon back sodium sulfonate, sulphuric acid, and the mass concentration of described carbon back sodium sulfonate is 5%, described sulfur
The mass concentration of acid is 4%, and described carbon back sodium sulfonate solution, the volume ratio of sulfuric acid solution are 6:5, the vine of described pretreatment
Granule is 1:6g/mL with the solid-liquid ratio of solution B；
S5: sugar liquid step S4 prepared accesses yeast, adds carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate,
Controlling temperature is 26 DEG C of bottom fermentations, prepares ethanol through dehydration；Described yeast quality is the 9% of sugar liquid quality, described carbamide, green
Semen Glycines powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality be respectively the 0.18% of sugar liquid quality, 0.02%, 0.16%, 0.08%,
0.14%。
Embodiment 3
A kind of method utilizing vine to prepare ethanol, comprises the following steps:
S1: the saline solution rinsing vine 2h using concentration to be 15%, after rinsing, vine is placed at temperature is 98-105 DEG C baking
Do to water content≤10%, prepare dry vine；
S2: dry vine step S1 prepared is pulverized, and crosses 200 mesh sieve, prepares vine granule；
S3: put in microwave reactor by the vine granule that step S2 prepares, adds solution A mix homogeneously, is heated to 68 DEG C,
Pretreatment 0.8h under speed of agitator is 300r/min, filters after being then cooled to room temperature, prepares the vine granule of pretreatment；
Described solution A is the mixed liquor of butanone, ethanol, ammonium phosphate salt, and the mass concentration of described butanone is 10%, the quality of described ethanol
Concentration is 80%, and the mass concentration of described ammonium phosphate salt is 8%, described butanone solution, ethanol solution, the volume of ammonium phosphate salt solution
It is 1:15g/mL than the solid-liquid ratio for 20:25:2, described vine granule and solution A；
S4: the vine granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mix homogeneously,
Regulation pH is 1.8, is heated to 78 DEG C, processes 1h under speed of agitator is 400r/min, filters, prepare after being then cooled to room temperature
Sugar liquid；Described solution B is the mixed liquor of carbon back sodium sulfonate, sulphuric acid, and the mass concentration of described carbon back sodium sulfonate is 7%, described sulphuric acid
Mass concentration be 6%, described carbon back sodium sulfonate solution, the volume ratio of sulfuric acid solution are 10:8, the vine of described pretreatment
Grain is 1:8g/mL with the solid-liquid ratio of solution B；
S5: sugar liquid step S4 prepared accesses yeast, adds carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate,
Controlling temperature is 28 DEG C of bottom fermentations, prepares ethanol through dehydration；Described yeast quality is the 11% of sugar liquid quality, described carbamide, green
Semen Glycines powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality be respectively the 0.22% of sugar liquid quality, 0.04%, 0.21%, 0.12%,
0.16%。
The sugar liquid taking out appropriate embodiment 1-3 is boiled, is inactivated, and appropriateness dilution, measures sugar fermentation by DNS colorimetric fixed sugar method
Content, and calculate sugar yield；The method of detection embodiment 1-3 prepares alcohol getting rate and purity simultaneously, and result is as shown in the table:
| Embodiment | Sugar yield, % | Alcohol getting rate, % | Purity of alcohol, % |
| 1 | 93.88 | 86.52 | 99.68 |
| 2 | 92.31 | 85.11 | 99.59 |
| 3 | 94.01 | 87.05 | 99.71 |
As seen from the above table, the method for embodiment of the present invention 1-3 the sugared yield prepared is 92.31%-94.01%, and alcohol getting rate is
85.11%-87.05%, purity of alcohol is 99.59%-99.71%, illustrates that sugar conversion ratio is high, and the method using the present invention can high yield
Ethanol.
Above content it cannot be assumed that the present invention be embodied as be confined to these explanation, technology belonging to the present invention is led
For the those of ordinary skill in territory, without departing from the inventive concept of the premise, it is also possible to make some simple deduction or replace,
All should be considered as belonging to the scope of patent protection that the present invention is determined by the claims submitted to.
Claims (8)
1. one kind utilizes the method that vine prepares ethanol, it is characterised in that comprise the following steps:
S1: use saline solution rinsing vine 2-3h, after rinsing, vine is placed at temperature is 98-105 DEG C drying to aqueous
Amount≤10%, prepares dry vine；
S2: dry vine step S1 prepared is pulverized, and sifts out, and prepares vine granule；
S3: put in microwave reactor by the vine granule that step S2 prepares, adds solution A mix homogeneously, is heated to 65-68
DEG C, pretreatment 0.5-0.8h under speed of agitator is 200-300r/min, filters after being then cooled to room temperature, prepares pretreatment
Vine granule；Described solution A is the mixed liquor of butanone, ethanol, ammonium phosphate salt, and the mass concentration of described butanone is 8%-10%,
The mass concentration of described ethanol is 60%-80%, and the mass concentration of described ammonium phosphate salt is 6%-8%, and described butanone solution, ethanol are molten
Liquid, the volume ratio of ammonium phosphate salt solution are 10-20:12-25:1-2, and described vine granule is 1:10-with the solid-liquid ratio of solution A
15g/mL；
S4: the vine granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mix homogeneously,
Regulation pH is 1.6-1.8, is heated to 75-78 DEG C, processes 1-1.5h, be then cooled under speed of agitator is 300-400r/min
Filter after room temperature, prepare sugar liquid；Described solution B is the mixed liquor of carbon back sodium sulfonate, sulphuric acid, and the quality of described carbon back sodium sulfonate is dense
Degree is 5%-7%, and the mass concentration of described sulphuric acid is 4%-6%, and described carbon back sodium sulfonate solution, the volume ratio of sulfuric acid solution are 6-
10:5-8, the vine granule of described pretreatment and the solid-liquid ratio of solution B are 1:6-8g/mL；
S5: sugar liquid step S4 prepared accesses yeast, adds carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate,
Controlling temperature is 26-28 DEG C of bottom fermentation, prepares ethanol through dehydration；Described yeast quality is the 9%-11% of sugar liquid quality, described
Carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality are respectively 0.18%-0.22%, 0.02%-of sugar liquid quality
0.04%、0.16%-0.21%、0.08%-0.12%、0.14%-0.16%。
The method utilizing vine to prepare ethanol the most according to claim 1, it is characterised in that Sal described in step S1
The concentration of water is 10%-15%.
The method utilizing vine to prepare ethanol the most according to claim 1, it is characterised in that sieve described in step S2
Mesh number be 120-200.
The method utilizing vine to prepare ethanol the most according to claim 1, it is characterised in that Fructus Vitis viniferae described in step S3
Rattan granule temperature of pretreatment in microwave reactor is 68 DEG C, and rotating speed is 300r/min, and the time is 0.5h.
The method utilizing vine to prepare ethanol the most according to claim 1, it is characterised in that Fructus Vitis viniferae described in step S3
Rattan granule is 1:12g/mL with the solid-liquid ratio of solution A.
The method utilizing vine to prepare ethanol the most according to claim 1, it is characterised in that locate in advance described in step S4
The vine granule of reason is 1.6 at the pH that ultrasound reactor processes, and temperature is 78 DEG C, and rotating speed is 400r/min, and the time is 1h.
The method utilizing vine to prepare ethanol the most according to claim 1, it is characterised in that locate in advance described in step S4
The vine granule of reason is 1:7g/mL with the solid-liquid ratio of solution B.
The method utilizing vine to prepare ethanol the most according to claim 1, it is characterised in that yeast described in step S5
Mycoplasma amount is the 10% of sugar liquid quality, and described carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality are respectively sugar liquid
The 0.2% of quality, 0.03%, 0.2%, 0.1%, 0.15%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107058406A (en) * | 2017-06-30 | 2017-08-18 | 合肥慧明瀚生态农业科技有限公司 | A kind of method that utilization timber waste prepares wood-based composites |
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| CN102352381A (en) * | 2011-10-08 | 2012-02-15 | 山东龙力生物科技股份有限公司 | Method using xylose production waste liquid to produce acetone and butanol |
| CN102876735A (en) * | 2012-07-25 | 2013-01-16 | 中国科学院成都生物研究所 | Method for producing acetone, ethanol and butanol by taking straw as raw material |
| CN102876736A (en) * | 2012-07-25 | 2013-01-16 | 中国科学院成都生物研究所 | Method for producing acetone, ethanol and butanol by taking straw as raw material |
| CN103436560A (en) * | 2013-08-21 | 2013-12-11 | 太仓碧奇新材料研发有限公司 | Preparation method for plant cirrus fermentation liquid glucose |
| CN104593448A (en) * | 2014-12-25 | 2015-05-06 | 山东龙力生物科技股份有限公司 | Method for producing ethanol from lignocellulosic biomass |
| CN104774877A (en) * | 2015-04-10 | 2015-07-15 | 山东龙力生物科技股份有限公司 | Method for co-producing ethanol, acetone and butanol by lignocellulose biomass |
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| CN107058406A (en) * | 2017-06-30 | 2017-08-18 | 合肥慧明瀚生态农业科技有限公司 | A kind of method that utilization timber waste prepares wood-based composites |
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