CN106092908B - A kind of rapid detection method of the fungimycin based on two color sensor dynamic white balances - Google Patents

Abstract

A kind of rapid detection method of the fungimycin based on two color sensor dynamic white balances, belongs to fungimycin detection field.For current Biological Detection fungimycin process it is more complicated, at high cost, not easy to operate the problems such as, propose a kind of method for quickly detecting fungimycin substance concentration using double color sensors, devise a colouring information detection system, dynamic white balance in solution example colouring information collection process is realized using dual sensor, to make color detection that there is adaptivity to the variation of environment light.Data obtained in collection process are handled using anti-impulse disturbances average value filtering algorithm, the final rgb value for obtaining solution example.Finally establish the linear relationship between rgb value and test sample concentration.The present invention makes adaptive ability of the detection system with stronger environment light.Experiments have shown that the detection method speed is fast, high-efficient, versatile, measurement accuracy and robustness with higher.

Description

A kind of quick detection of the fungimycin based on two color sensor dynamic white balances Method

Technical field

The present invention relates to a kind of rapid detection methods of fungimycin based on two color sensor dynamic white balances.

Background technique

Currently, food-safety problem is got worse, and the safety problem as caused by fungimycin substance is very universal.Very Verticillium toxin (mycotoxins) is metabolite caused by fungi grows in food or feed, it seriously threatens the mankind and moves The health and lives safety of object.Common mycotoxin mainly has aflatoxin (aflatoxins), ochratoxin A (ochratox A), fumarubicin (fumonisins) and zearalenone (zearalenone) etc..Due to most of true Mycomycin all has toxicity, and therefore, stringent limit has been formulated the content of fungimycin in food-related products in countries in the world Amount standard.

Aflatoxin in field of food safety, every gram of food is with 10^-9Gram for unit measure, have be detected ratio The features such as high, sample distribution is unevenly low with there are content.Therefore, higher want is proposed to limit standard and detection technique It asks.Currently, the conventional instrument detection method for mycotoxin mainly has thin-layered chromatography (TLC), high performance liquid chromatography (HPLC), the instrument analytical methods such as mass spectrometer (GCMS) and liquid chromatography mass spectrometric method (LCMS).Using a kind of high performance liquid chromatography Tandem mass spectrometry (HPLC-MS/MS) come measure analysis peanut and its product in five kinds of fungimycins, this method high sensitivity, Selectivity is good.But such methods are made a general survey of, generally existing sample pretreatment process is more complicated, instrument and equipment is expensive, need to specially grasp The problems such as making personnel and taking time and effort, to limit its scope of application, and conventional instrument detection method is not able to satisfy scene The demand of rapid screening analysis.Therefore, efficient mycotoxin detection pre-treatment and novel Fast Detection Technique and equipment are developed It is particularly important.It is recently proposed a kind of quick detection side based on non-marked aptamer Visual retrieval aflatoxin B1 Method, caused color change after being agglomerated under Salt treatment using nanogold are realized for the sensitive quick of aflatoxin B1 Visual retrieval.But be based on colorimetric determination when, due to different batches nanogold 520 nanometer wave strong points absorbance Slightly difference, when aptamers and salt ionic concentration are not fixed in solution, absorbance can all be affected, so having to testing result Certain influence.Currently, antigen-antibody method, i.e. immunological method are also used for the quick detection to mycotoxin.But it is this Method needs to prepare the antibody of specificity, and antibody itself is due to unstability, so the cost that should not be saved and prepare It is higher.These deficiencies constrain extensive use of the immunological method in mycotoxin field.

In above-described various methods, detection limit is universal higher, and unsuitable mycotoxin haves the characteristics that content is low. Therefore the sensitivity of detection method is continuously improved, enhancing anti-interference ability, improves detection speed, and reduces testing cost always It is all the driving source of mycotoxin Fast Detection Technique exploitation.

Summary of the invention

The present invention is studied for the detection of fungimycin content, is proposed a kind of yellow bent for field quick detection The technology of mould toxin.Herein for mycotoxin, a kind of detection method for determining solution concentration using solution colour is proposed, is led to Three primary colors (RGB) value for obtaining solution colour using color sensor is crossed, realizes the matching of three primary colors and various concentration solution, from And establish the linear relationship between solution concentration and rgb value.This method improves traditional single-sensor color acquisition system simultaneously System realizes the dynamic white balance of environment light using dual sensor, and detection system is made to have the adaptive ability of stronger environment light. Experiments have shown that the detection method speed is fast, high-efficient, versatile, measurement accuracy and robustness with higher.

A kind of rapid detection method of the fungimycin based on two color sensor dynamic white balances, it is characterised in that:

Color sensor I passes through three Color Channels of RGB, utilizes white light in timer/counter acquisition environment Initial data；

Color sensor II utilizes the color data of timer/counter acquisition solution by tri- Color Channels of RGB；

Using Mean Filtering Algorithm, the initial data of white light in environment light is filtered, environment light is obtained White balance parameter；

Sensor is in data acquisition, and there are pulse interference signals, carries out anti-arteries and veins to the solution colour data of acquisition Punching interference average value filtering processing；

The white balance parameter obtained using sensor I is as the rgb value of benchmark solution colour；

Relationship between the two is established with corresponding rgb value according to sample concentration, by the linear relationship having built up, then The colouring information that detection unknown concentration solution is removed using two color sensor systems, finally extrapolates corresponding concentration value.

The correspondence reference frequency of RGB white in the detected environment light of sensor I in Fig. 1 is set to F_r、F_g、 F_b, T_r、T_g、T_bThe correspondence reference period of respectively white RGB, sample frequency difference when II test sample color of sensor It is set as f_r、f_g、f_b, t_r、t_g、t_bSampling period when for test sample color utilizes formula according to the testing principle of color sensor (1), (2) and (3) respectively obtain the rgb value of solution example.

Wherein, since color sensor can not directly acquire measurement frequency in the detection process, so system passes through detection Cycle T and t calculate rgb value.It before calculating RGB, needs that cycle T and t is once filtered, wherein T, t distinguish Two sensors corresponding period when for sampling pulse signal.N is sampling number, T_iDuring acquisition environment light pulse signal A wherein period, t_iTo obtain wherein period during sample pulse signal；

Using two color sensors during detecting color, the automation adjustment of detection data, tool can be realized There is environment self-adaption.Experimental data proof diagram is as follows:

The invention has the following beneficial effects:

(1) feature is automated.Two Color Detecting Systems that the present invention uses are able to achieve the automation feature of color detection, When environment light changes, it can adjust automatically, guarantee the accuracy of testing result, there is environment self-adaption sexual function；

(2) portability feature.Scene, detection on the spot, easy to use, popularization may be implemented in the detection device；

(3) easy to operate, quick.By established linear relationship, it can directly pass through detection unknown concentration solution Its corresponding concentration can be obtained in colouring information；

(4) high sensitivity.By detecting unknown concentration, its relative error is obtained lower than 7%, meets the accurate of detection data Degree requires；

(5) at low cost, profitable.This detection device production cost is low, greatly reduces testing cost.

For the detection of mycotoxin, the quick inspection proposed in this paper that dynamic white balance is realized using two color sensors Survey method does not need the antibody of preparation specificity yet, only needs independent of the sample pre-treatments of expensive instrument and equipment and complexity By acquiring the color change information with analytical solution, the linear relationship between solution concentration and three primary colors (RGB) is established.Together When, due to realizing automatically correcting in real time for white balance, avoiding the change pair of environment light using two color sensor measurements The influence of testing result ensure that the stability of detection system and the accuracy of testing result.The experiment show detection The feasibility and validity of method.This method can be equally used for detecting other in relation to candex kind object matter.

Detailed description of the invention

The realization of Fig. 1 dynamic white balance

The variation of rgb value under Fig. 2 varying environment light

Scheming (a) and (b) is the situation of change for the R value that single, double sensor measures under two kinds of varying environment light.

Scheming (c) and (d) is the situation of change for the G value that single, double sensor measures under two kinds of varying environment light.

Scheming (e) and (f) is the situation of change for the B value that single, double sensor measures under two kinds of varying environment light.

Conclusion:

1, rgb value of the same sample under varying environment light can be fluctuated and be changed；

2, when environment light changes, the data fluctuations that Method for Single Sensor System collects are bigger；

3, when environment light changes, the data fluctuations that double-sensor system collects are smaller.

Fig. 3 is Color Detecting System circuit diagram, and wherein color sensor model TCS230, voltage conversion chip are The power supply line of ULN2003, color sensor carry out decoupling using 0.01 μ of μ F -0.1 F, and the enable end OE pin of chip draws with GND It should be connected using Low ESR between foot, to improve noise resisting ability.Fig. 2 is solution example to be detected, is loaded in centrifuge tube, molten Liquid product accounts for the 85% of centrifugation pipe volume.

Specific embodiment

The preparation of reagent mainly has the preparation of nanogold, AFB1 aptamers and nanogold under high salt concn in the present invention Effect.

1, the preparation of nanogold

Experiment prepares colloidal gold using trisodium citrate reduction method, and 95.8mL ultrapure water is added in three neck round bottom flask, 1% chlorauric acid solution of 4.2mL mass fraction is added, magnetic force is heated at reflux stirring, persistently boils 10min, 10mL is then added 1% citric acid three sodium solution of mass fraction, solution colour redden in 1min, continue the 20min that boils, naturally cold under stirring But to room temperature, obtained claret liquid, as nanogold.

2, the preparation of solution example

The concentration of aflatoxin B1 (AFB1) aptamers and the concentration of NaCl salting liquid are this detection method reaction systems Key factor pass through the aptamers and NaCl to various concentration since the length of different aptamers, base composition are different The comparison of solution, has determined optimum reaction condition.The optium concentration that aptamers have been determined is 10 μm of ol/L, and concentration is 2mol/L's NaCl solution.

Detecting step

Two innovative color sensor systems that this system uses during obtaining color sample information, are realized Automation in detection process, has the function of adaptivity, improves stability, the accuracy of detection data.It realizes simultaneously A method of new detection aflatoxin concentration, the specific steps are as follows:

Color sensor I passes through three Color Channels of RGB, utilizes white light in timer/counter acquisition environment Initial data；

Color sensor II utilizes the color data of timer/counter acquisition solution by tri- Color Channels of RGB；

Using Mean Filtering Algorithm, the initial data of white light in environment light is filtered, environment light is obtained White balance parameter；

Sensor is in data acquisition, and there are pulse interference signals, so the solution colour data to acquisition carry out Anti- impulse disturbances average value filtering processing；Remove maximum value and minimum value and carries out average value filtering processing again；

The white balance parameter obtained using sensor I is as the rgb value of benchmark solution colour；

Relationship between the two is established with corresponding rgb value according to sample concentration, can be obtained by established linear relationship To the solution of unknown concentration.

By the linear relationship having built up, detection unknown concentration solution then is removed using two color sensor systems Colouring information finally extrapolates corresponding concentration value.

Claims (1)

1. a kind of rapid detection method of the fungimycin based on two color sensor dynamic white balances, it is characterised in that:

Color sensor I by three Color Channels of RGB, using in timer and counter acquisition environment white light it is original Data；

Color sensor II utilizes the color data of timer and counter acquisition solution by tri- Color Channels of RGB；

Using Mean Filtering Algorithm, the initial data of white light in environment light is filtered, obtains the white flat of environment light Weigh parameter；

Sensor is in data acquisition, and there are pulse interference signals, and it is dry to carry out anti-pulse to the solution colour data of acquisition Disturb average value filtering processing；

The white balance parameter obtained using sensor I is as the rgb value of benchmark solution colour；

It is then utilized with the relationship that corresponding rgb value is established between the two by the linear relationship having built up according to sample concentration Two color sensor systems remove the colouring information of detection unknown concentration solution, finally extrapolate corresponding concentration value.