CN106063523A - Promote NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum and the application thereof of Penaeus vannamei growth - Google Patents
Promote NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum and the application thereof of Penaeus vannamei growth Download PDFInfo
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- CN106063523A CN106063523A CN201510184090.9A CN201510184090A CN106063523A CN 106063523 A CN106063523 A CN 106063523A CN 201510184090 A CN201510184090 A CN 201510184090A CN 106063523 A CN106063523 A CN 106063523A
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- new type
- probiotic bacteria
- polysaccharide gum
- composite probiotic
- penaeus vannamei
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention discloses a kind of NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum promoting Penaeus vannamei to grow and application thereof, preparation promote Penaeus vannamei growth NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solution for by former for bacillus marinus powder, gulp down the former powder of bacterium vibrio, carrageenan, xanthan gum, guar gum, Konjac glucomannan, artemisia glue by uniform stirring mix, become solution with water dissolution；The application NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum preparation feedstuff containing NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum is to be mixed with normal feedstuff uniform stirring by NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solution, and room temperature dries at cool place.What the present invention provided promotes NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum and the application thereof of Penaeus vannamei growth, compared with traditional antibiotic, safer, Penaeus vannamei will not be caused to create serious drug resistance and drug residue, growth to Penaeus vannamei has significantly more facilitation, is a kind of new product that can effectively facilitate Penaeus vannamei growth and quality safety.
Description
Technical field
The present invention relates to a kind of biological polyoses glue, be specifically related to a kind of NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum promoting Penaeus vannamei to grow and application thereof.
Background technology
Penaeus vannamei is one of the highest three big excellent shrimp species of cultured output of generally acknowledging of the world today, extensively cultivates in China.But, fast development and the continuous expansion of cultivation scale along with culture of Penaeus vannamei industry, raiser is frequently excessively used the misconduct of antibiotic makes Penaeus vannamei create serious drug resistance and drug residue, seriously constrains the growth of culture of Penaeus vannamei industry.Therefore, safe Substitutes For Antibiotic is sought particularly important to ensure that Penaeus vannamei has good growth performance.In view of this, the present invention is prepared for a kind of NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum promoting Penaeus vannamei to grow and application thereof.Said preparation is compared with traditional antibiotic, safer, Penaeus vannamei will not be caused to create serious drug resistance and drug residue, and the growth to Penaeus vannamei has significantly more facilitation, is a kind of new product that can effectively facilitate Penaeus vannamei growth and quality safety.
Summary of the invention
The technical problem to be solved is to provide a kind of NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum promoting Penaeus vannamei to grow and application thereof, compared with traditional antibiotic, safer, Penaeus vannamei will not be caused to create serious drug resistance and drug residue, growth to Penaeus vannamei has significantly more facilitation, is a kind of new product that can effectively facilitate Penaeus vannamei growth and quality safety.
The present invention solves that above-mentioned technical problem employed technical scheme comprise that a kind of NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum promoting Penaeus vannamei to grow of offer and application thereof, wherein:
Preparation promote the process of NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solution of Penaeus vannamei growth be by former for bacillus marinus powder, gulp down the former powder of bacterium vibrio, carrageenan, xanthan gum, guar gum, Konjac glucomannan, artemisia glue are mixed by uniform stirring, become solution with water dissolution；
The application NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum preparation feedstuff process containing NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum is to be mixed with normal feedstuff uniform stirring by NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solution, and room temperature dries at cool place.
The above-mentioned NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum promoting Penaeus vannamei growth and application thereof, wherein, during preparation promotes the NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solution of Penaeus vannamei growth, the former powder of bacillus marinus, gulp down the former powder of bacterium vibrio, carrageenan, xanthan gum, guar gum, Konjac glucomannan, artemisia glue in ratio be: the former powder of bacillus marinus 15%, gulp down the former powder of bacterium vibrio 10%, carrageenan 20%, xanthan gum 10%, guar gum 20%, Konjac glucomannan 20%, artemisia glue 5%；
Concrete operations are: first by 2 kilograms of carrageenans, 2 kilograms of guar gum 100L80 DEG C water uniform stirrings dissolve, mixing speed is 20r/min, mixing time is 60min, 1 kilogram of xanthan gum is added the most again in water, 2 kilograms of Konjac glucomannan, 0.5 kilogram of artemisia glue uniform stirring at normal temperatures mixing, mixing speed is 20r/min, mixing time is 90min, last in lysate, add 1.5 kilograms of former powder of bacillus marinus again and 1 kilogram gulp down the former powder of bacterium vibrio, mixing speed is 20r/min, mixing time is 60min, triple mixing mixed process is in air-tight state.
The above-mentioned NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum promoting Penaeus vannamei growth and application thereof, wherein, during application NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum preparation is containing NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum feedstuff, NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solution is mixed with normal feedstuff uniform stirring according to mass ratio 1: 5, concrete operations are: first by 2 kilograms of NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solutions and 10 kilograms of normal feedstuff uniform stirring mixing, mixing speed is 15r/min, and mixing time is 30min.
The present invention has the advantages that relative to prior art
The application NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum preparation feedstuff containing NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum has good growth-promoting effect to Penaeus vannamei, the effect improving culture of Penaeus vannamei rate of body weight gain and survival rate is significantly better than conventional antibiotic norfloxacin hydrochloride powder, and it is safer, Penaeus vannamei will not be caused to create serious drug resistance and drug residue, be a kind of new product that can effectively facilitate Penaeus vannamei growth and quality safety.
Accompanying drawing explanation
The NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum of the promotion Penaeus vannamei growth that Fig. 1 provides for the present invention and the application effect comparison table of application thereof.
Detailed description of the invention
The invention will be further described with embodiment below in conjunction with the accompanying drawings.
The NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum of the promotion Penaeus vannamei growth that the present invention provides and application experiment material thereof:
The former powder of bacillus marinus, concentration is more than 100,000,000/gram；Gulping down the former powder of bacterium vibrio, concentration is more than 10,000,000/gram；Carrageenan, effective content 99%；Xanthan gum, effective content 98%；Guar gum, effective content 99%；Konjac glucomannan, effective content 98%；Artemisia glue, effective content 98%；Norfloxacin hydrochloride powder, effective content 98%；Penaeus vannamei normal feedstuff and the pharmaceutical feed being added with 10% norfloxacin hydrochloride；Penaeus vannamei Seedling, totally 1500 tail, original body mass 1.23g/ tail.
The NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum of the promotion Penaeus vannamei growth that the present invention provides and the concrete operations scheme of application thereof are as described below:
Promote that about preparation the process of the NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solution of Penaeus vannamei growth is as follows；
By former for bacillus marinus powder, gulp down the former powder of bacterium vibrio, carrageenan, xanthan gum, guar gum, Konjac glucomannan, the mixing of artemisia glue uniform stirring, become solution to be the NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solution promoting that Penaeus vannamei grows with water dissolution.Being preferably in a proportion of of each of which composition: the former powder of bacillus marinus 15%, gulp down the former powder of bacterium vibrio 10%, carrageenan 20%, xanthan gum 10%, guar gum 20%, Konjac glucomannan 20%, artemisia glue 5%.Concrete operations are: first 2 kilograms of carrageenans, 2 kilograms of guar gum 100L 80 DEG C water uniform stirrings are dissolved, mixing speed is 20r/min, mixing time is 60min, 1 kilogram of xanthan gum, 2 kilograms of Konjac glucomannan, 0.5 kilogram of artemisia glue uniform stirring at normal temperatures mixing are added the most again in water, mixing speed is 20r/min, mixing time is 90min, last in lysate, add 1.5 kilograms of former powder of bacillus marinus again and 1 kilogram gulp down the former powder of bacterium vibrio, mixing speed is 20r/min, and mixing time is 60min.Triple mixing mixed process is in air-tight state.
The application NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum preparation process containing NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum feedstuff is as follows:
Being mixed with normal feedstuff uniform stirring by NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solution, preferably stirring mixed proportion is to be mixed with normal feedstuff uniform stirring according to mass ratio 1: 5 by NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solution, and room temperature dries at cool place.Concrete operations are: first by 2 kilograms of NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solutions and 10 kilograms of normal feedstuff uniform stirring mixing, mixing speed is 15r/min, and mixing time is 30min, dries at room temperature cool place the most again.
What the present invention provided promotes that the NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum of Penaeus vannamei growth and the application effect of application thereof are: cultivated by 1500 tail Penaeus vannamei Seedlings in the culturing pool that 3 capacity are 6 cubic metres, each culturing pool 500 tail.Wherein, 1 blank pond, normal feedstuff of throwing something and feeding；1 positive control pond, pharmaceutical feed of throwing something and feeding；1 test tank, the feedstuff containing NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum of throwing something and feeding.Culturing pool takes from natural cultivation water, and throw something and feed every day 2 times (7: 30 and 16: 30), feeding volume is the 3% of TBW, suitably can adjust according to the situation of ingesting.Water, dissolved oxygen content ＞ 5mg/L is not changed during cultivation.Survival rate and rate of body weight gain is measured after off-test.The computing formula of survival rate and rate of body weight gain is respectively as follows: survival rate=off-test shrimp mantissa/test Chu Xia mantissa × 100%；Rate of body weight gain=(end counterpoise-first counterpoise)/the most all plant × 100%.
Experimental result is as shown in the form of accompanying drawing 1.Test result indicate that, promote the compound probiotic polysaccharide gum of Penaeus vannamei growth that Penaeus vannamei is grown and survived and there is remarkable result.The Penaeus vannamei survival rate of the feedstuff containing NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum of throwing something and feeding and rate of body weight gain are respectively 93.6% and 397.56%, and the survival rate of the Penaeus vannamei of throw something and feed normal feedstuff and pharmaceutical feed and rate of body weight gain are respectively 64.8% and 360.98%, 68.4% and 371.54%.Thus illustrate, containing promoting that the feedstuff of compound probiotic polysaccharide gum of Penaeus vannamei growth is to growth-promoting effect traditional infrastructure the to be substantially better than feedstuff of Penaeus vannamei and pharmaceutical feed.
Although the present invention discloses as above with preferred embodiment; so it is not limited to the present invention, any those skilled in the art, without departing from the spirit and scope of the present invention; when making a little amendment and perfect, therefore protection scope of the present invention is when with being as the criterion that claims are defined.
Claims (3)
1. one kind promotes Penaeus vannamei to grow NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum and application thereof, it is characterised in that:
Preparation promotes that the process of the NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solution of Penaeus vannamei growth is: by ocean bud
The former powder of spore bacillus, gulp down the former powder of bacterium vibrio, carrageenan, xanthan gum, guar gum, Konjac glucomannan, artemisia glue by uniformly
Stirring mixing, becomes solution with water dissolution；
The application NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum preparation process containing NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum feedstuff is: will be new
Type compound probiotic polysaccharide gum aqueous solution mixes with normal feedstuff uniform stirring, and room temperature dries at cool place.
A kind of NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum promoting Penaeus vannamei to grow the most as claimed in claim 1 and
Its application, it is characterised in that:
During preparation promotes the NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solution of Penaeus vannamei growth, ocean spore
The former powder of bacillus, gulp down the former powder of bacterium vibrio, carrageenan, xanthan gum, guar gum, Konjac glucomannan, artemisia glue in ratio
For: the former powder of bacillus marinus 15%, gulp down the former powder of bacterium vibrio 10%, carrageenan 20%, xanthan gum 10%, Guar beans
Glue 20%, Konjac glucomannan 20%, artemisia glue 5%；
Concrete operations are: first by 2 kilograms of carrageenans, 2 kilograms of guar gum 100L80 DEG C water uniform stirrings
Dissolving, mixing speed is 20r/min, and mixing time is 60min, the most again in water
Add 1 kilogram of xanthan gum, 2 kilograms of Konjac glucomannan, 0.5 kilogram of artemisia glue uniform stirring at normal temperatures mixing, stirring
Speed is 20r/min, and mixing time is 90min, adds 1.5 kilograms of ocean buds the most again in lysate
The former powder of spore bacillus and 1 kilogram gulp down the former powder of bacterium vibrio, and mixing speed is 20r/min, and mixing time is 60min,
Triple mixing mixed process is in air-tight state.
A kind of NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum promoting Penaeus vannamei to grow the most as claimed in claim 2 and
Its application, it is characterised in that:
During application NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum preparation is containing NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum feedstuff, will be new
Type compound probiotic polysaccharide gum aqueous solution mixes with normal feedstuff uniform stirring according to mass ratio 1: 5, and concrete operations are:
First by 2 kilograms of NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum aqueous solutions and 10 kilograms of normal feedstuff uniform stirring mixing, stirring
Speed is 15r/min, and mixing time is 30min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510184090.9A CN106063523A (en) | 2015-04-20 | 2015-04-20 | Promote NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum and the application thereof of Penaeus vannamei growth |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510184090.9A CN106063523A (en) | 2015-04-20 | 2015-04-20 | Promote NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum and the application thereof of Penaeus vannamei growth |
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| CN106063523A true CN106063523A (en) | 2016-11-02 |
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| CN201510184090.9A Pending CN106063523A (en) | 2015-04-20 | 2015-04-20 | Promote NEW TYPE OF COMPOSITE probiotic bacteria polysaccharide gum and the application thereof of Penaeus vannamei growth |
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Citations (7)
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| CN101157914A (en) * | 2007-09-20 | 2008-04-09 | 西北师范大学 | Bentonite, biological polysaccharide plural gel immobilized cell carrier and preparation method thereof |
| CN102459568A (en) * | 2009-05-26 | 2012-05-16 | 先进生物营养公司 | Stable dry powder composition comprising biologically active microorganisms and/or bioactive materials and methods of making |
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| CN103947866A (en) * | 2014-04-02 | 2014-07-30 | 林震能 | Gel fish feed |
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| CN101157914A (en) * | 2007-09-20 | 2008-04-09 | 西北师范大学 | Bentonite, biological polysaccharide plural gel immobilized cell carrier and preparation method thereof |
| CN102459568A (en) * | 2009-05-26 | 2012-05-16 | 先进生物营养公司 | Stable dry powder composition comprising biologically active microorganisms and/or bioactive materials and methods of making |
| CN102481371A (en) * | 2009-07-28 | 2012-05-30 | 卫泰克实验室公司 | Sticky Soft Gel For Treating Poultry |
| CN102517237A (en) * | 2011-12-30 | 2012-06-27 | 湖南农业大学 | Composite micro-ecological water-based suspension concentrate for aquaculture |
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| CN103947866A (en) * | 2014-04-02 | 2014-07-30 | 林震能 | Gel fish feed |
| CN104450576A (en) * | 2014-12-10 | 2015-03-25 | 苏州埃瑞特生物技术有限公司 | Liquid preparation of bacillus pumilus and bdellovibrio and application thereof |
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Application publication date: 20161102 |
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