CN105997000B - Raman spectrum detection device based on fiber endoscope and implementation method thereof - Google Patents
Raman spectrum detection device based on fiber endoscope and implementation method thereof Download PDFInfo
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- CN105997000B CN105997000B CN201610375997.8A CN201610375997A CN105997000B CN 105997000 B CN105997000 B CN 105997000B CN 201610375997 A CN201610375997 A CN 201610375997A CN 105997000 B CN105997000 B CN 105997000B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/06—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
- A61B1/07—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements using light-conductive means, e.g. optical fibres
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/00163—Optical arrangements
- A61B1/00165—Optical arrangements with light-conductive means, e.g. fibre optics
- A61B1/00167—Details of optical fibre bundles, e.g. shape or fibre distribution
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/233—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor for the nose, i.e. nasoscopes, e.g. testing of patency of Eustachian tubes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/307—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor for the urinary organs, e.g. urethroscopes, cystoscopes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/313—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor for introducing through surgical openings, e.g. laparoscopes
- A61B1/317—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor for introducing through surgical openings, e.g. laparoscopes for bones or joints, e.g. osteoscopes, arthroscopes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/48—Other medical applications
- A61B5/4887—Locating particular structures in or on the body
Abstract
The invention relates to a Raman spectrum detection device based on a fiber endoscope and an implementation method thereof, wherein the Raman spectrum detection device comprises a dual-wavelength laser, a Raman fiber probe, the fiber endoscope, a white-light cold light source, a camera device, a Raman spectrometer and a display device; the white light cold light source is connected with an optical interface of the fiber endoscope, the camera device is arranged at the upper part of the fiber endoscope and used for collecting images in the fiber endoscope, and the output end of the camera device is connected with the display device and used for displaying the images in the fiber endoscope; the output end of the dual-wavelength laser is connected with the input end of the Raman fiber probe; and the output end of the Raman fiber probe is connected with the Raman spectrometer and the detector thereof. The invention is suitable for the living body and real-time Raman spectrum detection and analysis of the tissue in the human body cavity.
Description
Technical Field
The invention relates to a fiber in the field of endoscopes and, more particularly, in particular to a Raman spectrum detection device based on a fiber endoscope and an implementation method thereof.
Background
Cancer is a disease that seriously threatens human health and has become the leading cause of death associated with human diseases. The cure rate of cancer is closely related to the stage of cancer discovery, and if the cure rate is found in an early stage, the cure rate is greatly improved. However, many cancer patients do not have obvious symptoms in the early stage, it is very difficult to find. <xnotran> . </xnotran> The fiberscope is a common medical instrument, enters the cavity of a human body through the cavity of the human body or a small incision made by operation, observes pathological changes in the cavity, determines the position and the range of the pathological changes, can perform operation and camera shooting, is a reliable tool for diagnosis and treatment, and is widely applied clinically.
However, most of the existing medical fiber endoscope detection systems rely on the traditional white light reflection fiber endoscope (such as fiber endoscope, electronic fiber endoscope, etc.) to observe the morphological lesion of cancer, and during diagnosis, the medical fiber endoscope detection systems only rely on the visual observation of doctors, combine personal experience, the structure and the shape of the abnormal part of the tissue are judged and identified, and the tiny tissue lesion can be difficult to observe, so that the diagnosis rate is greatly reduced, the missed diagnosis or the misdiagnosis is caused, and certain difficulties exist in the clinical diagnosis of early cancer. The Raman spectrum is an inelastic scattering spectrum, can obtain fingerprint information such as molecular structures, vibration modes, functional groups and the like rich in substances without a complex sample preparation process, has the advantages of small water interference in biological tissues, high sensitivity to biochemical component changes of proteins, nucleic acids, phospholipids and sugar and the like, can be widely applied to analysis of biological molecular structures, and is an optical detection technology with no damage, high speed and high sensitivity.
The current research shows that the fingerprint area (200-2000 cm) of Raman spectrum -1 Finger print has diagnostic significance on diseases of human tissues, and the high wave number region (2600-3500 cm) -1 High wave number) also provides very important biochemical information. Therefore, in the case of performing raman spectroscopy of human tissue, it is necessary to acquire raman spectra of a fingerprint region and a high-frequency region at the same time. However, in order to obtain a raman spectrum in a larger spectral range, a common method is to use a detector with a larger area to receive raman spectrum signals in a wider wavenumber range. <xnotran> , , , . </xnotran>
On the other hand, instruments entering the body cavity through the fiberscope detection channel, such as medical instruments which touch the mucosa and even cause the mucosa to be damaged and bleed due to improper operation, are invasive instruments according to the classification rule of medical instrument classification rule, wherein the medical instruments wholly or partially enter the body through the body surface and contact tissues in the body, a blood circulation system, a central nervous system and the like. It is therefore important to ensure that the viewing apparatus entering the body cavity does not cause damage to the body. One possible solution is to limit the length of the device that is inserted into the body cavity, i.e. to add a stop tissue to avoid contact with the tissue due to improper handling by the person.
Disclosure of Invention
In view of this, the present invention aims to provide a raman spectroscopy detection apparatus based on a fiber endoscope and a realization method thereof, and aims to provide an apparatus suitable for living body of tissue in a human body cavity and for real-time raman spectroscopy detection and diagnosis.
The invention is realized by adopting the following scheme: a Raman spectrum detection device based on a fiber endoscope, the system comprises a dual-wavelength laser, a Raman fiber probe, a fiber endoscope, a white light cold light source, a camera device, a Raman spectrometer and a data processing and displaying device; the white light cold light source is connected with an optical interface of the fiber endoscope, the camera device is arranged at the upper part of the fiber endoscope and is used for collecting images in the fiber endoscope, the output end of the camera device is connected with the data processing and displaying device and is used for displaying the image in the fiberscope; <xnotran> ; </xnotran> The output end of the Raman fiber probe is connected with the Raman spectrometer and the detector thereof.
<xnotran> , Y , , , , . </xnotran>
Furthermore, the beam-combining optical fibers arrange a plurality of collecting optical fibers around the excitation optical fibers on the end face of the detection end part in a circumferential manner, and the detection end part is fixed by adopting a metal sleeve.
Furthermore, an adjustable limiting device is arranged at a position, close to the branch, of the bundled optical fiber of the raman optical fiber probe, the limiting device is used for adjusting the length of the optical fiber probe entering the detection channel of the fiber endoscope to limit, the limiting device comprises a fixing sleeve and a fixing screw, the fixing sleeve is sleeved on the surface of the raman optical fiber probe, and the fixing screw is vertically arranged on the fixing sleeve and used for fixing the raman optical fiber probe.
Further, the excitation optical fiber and the collection optical fiber are both wrapped by a polymer material.
Further, the fiber end face of the detection end part of the raman fiber probe is provided with a coating, the end face of the excitation fiber is coated with a low-pass film allowing excitation light with two wavelengths to pass through, and the end face of the collection fiber is coated with a high-pass film for cutting off the excitation light and allowing raman scattered light with a larger wavelength to pass through.
Furthermore, the output optical fiber of the dual-wavelength laser is connected with the excitation optical fiber of the Raman optical fiber probe through a filtering component, so as to alternately output excitation light with two different wavelengths; the collection optical fiber of the Raman optical fiber probe is connected with the filtering component, and the output light of the Raman optical fiber probe is connected with the Raman spectrometer and the detector thereof through the collection optical fiber.
Further, the dual-wavelength laser alternately outputs two types of excitation light with different wavelengths, wherein the two types of excitation light comprise 785nm excitation light and 690nm excitation light; the 785nm exciting light is used for detecting the Raman spectrum of the fingerprint region, and the 690nm exciting light is used for detecting the Raman spectrum of the high wavenumber region.
Furthermore, the fiberscope is a fiberscope which can meet different positions in the human body cavity, and comprises a nasopharyngoscope, a cystoscope, a hysteroscope, a vocal cord arthroscope and a ureter-nephroscope.
<xnotran> , 300W . </xnotran>
Furthermore, the data processing and displaying device is a PC.
The invention is also realized by adopting the following method: a realization method of a Raman spectrum detection device based on a fiber endoscope is characterized in that: comprises that the following are provided the method comprises the following steps:
step S1: the Raman fiber probe is extended into a detection channel of the fiber endoscope, the length of the fiber endoscope entering the detection channel is adjusted by adopting a limiting device, and the fiber endoscope is extended into a human body cavity for detection;
step S2: turning on a white light cold light source, arranging the camera device on the upper part of the fiber endoscope, and collecting images in the fiber endoscope; connecting the output end of the camera device with the data processing is connected with the display device, to display an image within the fiberscope;
and step S4: and starting the dual-wavelength laser, emitting exciting light with two different wavelengths, enabling the exciting light to enter through an exciting optical fiber, collecting the Raman scattering light collected by a collecting optical fiber and transmitting the Raman scattering light to the Raman spectrometer and the detector thereof, and finishing the detection of the Raman spectrum in the fingerprint area and the detection of the Raman spectrum in the high-wave-number area.
Further, the air conditioner is characterized in that, in the obtained Raman spectrum characteristics of living human nasopharyngeal carcinoma tissues and normal nasopharyngeal carcinoma tissues,
in the fingerprint area: raman spectra of normal and tumor tissues are 851, 943, 1004, 1096, 1124, 1265, 1316, 1450, 1621 and 1660cm -1 Has obvious Raman peak; compared with the normal tissues, the tissue-specific tumor suppressor has the advantages that, the Raman spectrum characteristic of the fingerprint area of the nasopharyngeal darcinoma tissue also changes obviously, that is, the peaks at 851, 943, 1096, 1124 dropped, while at 1004, 1265, 1316, 1450, 1621, and 1660cm -1 The peak at (a) rises; the shape of the spectrum of normal tissue and tumor tissue is 1120-1360 cm -1 And 1560-1680cm -1 There are also significant differences between the intervals.
At high wave number of zone (b): the Raman spectra of normal and nasopharyngeal carcinoma tissues are respectively 2854 cm, 2940 cm and 3009 cm -1 And 3067 cm -1 Has obvious Raman peak; the Raman spectrum characteristics of the nasopharyngeal carcinoma tissue in the high wave number region are obviously changed compared with the normal tissue, namely the spectrum of the nasopharyngeal carcinoma tissue is 28 times that of the normal tissue54、2940 cm -1 The peak at (a) rises.
Compared with the prior art, the method has the advantages that, the invention is provided with the advantages are as follows: the Raman spectrum detection device based on the fiber endoscope, which is established by the invention, adopts the specially designed optical fiber probe, can conveniently enter the human body cavity through the detection channel of the fiber endoscope, and carries out Raman spectrum measurement on the tissues in the human body cavity; the dual-wavelength laser is adopted to alternately output excitation with two different wavelengths as exciting light, and the simultaneous detection of the Raman spectrum of the fingerprint area and the Raman spectrum of the high-wave-number area is realized by the same spectral detector with a smaller area in cooperation with the filtering component and the control software. In addition, the Raman spectrum detection device can realize nondestructive, real-time and efficient Raman spectrum detection of the tissues in the human body cavity; the Raman spectrum detection system has the advantages of wide wave number coverage range, small size and the like, thereby providing an effective clinical detection tool for nondestructive and rapid analysis and diagnosis of living tissues and having important application value. Meanwhile, by utilizing the Raman spectrum detection device and detection method based on the fiber endoscope, which are established by the invention, the Raman spectrum characteristics of the fingerprint area and the high-wave number area of living human nasopharyngeal carcinoma tissues and normal nasopharyngeal carcinoma tissues and the difference between the fingerprint area and the high-wave number area are obtained.
Drawings
FIG. 1 is a fiber-based endoscope the raman spectroscopy detection device of (1).
Fig. 2 is a block diagram of the fiber optic probe of fig. 1.
Fig. 3 is a schematic diagram of the structure of the position-limiting device in the fiber probe of fig. 2.
FIG. 4 is a schematic diagram of a plated film on the end face of the optical fiber at the probing end of the optical fiber probe shown in FIG. 2.
FIG. 5 is a comparison graph of the average Raman spectrum of the nasopharyngeal tissue at the normal position and the average Raman spectrum of the nasopharyngeal cancer tissue at a low wavenumber obtained under the excitation of 785nm excitation light.
FIG. 6 is a graph showing the comparison of the average Raman spectrum of the nasopharyngeal tissue at the normal position and the average Raman spectrum of the nasopharyngeal cancer tissue at a high wavenumber obtained under the excitation of 690nm excitation light
Detailed Description
The following description and examples are given in connection with the accompanying drawings the present invention is further explained.
The present embodiment provides a raman spectroscopy apparatus based on a fiber endoscope, as shown in fig. 1, the system comprises a dual-wavelength laser, a Raman fiber probe, a fiber endoscope, a white light cold light source, a camera device, a Raman spectrometer and a data processing and displaying device; the white light cold light source is connected with an optical interface of the fiber endoscope, the camera device is arranged at the upper part of the fiber endoscope and used for collecting images in the fiber endoscope, and the output end of the camera device is connected with the data processing and displaying device and used for displaying the images in the fiber endoscope; the output end of the dual-wavelength laser is connected with the input end of the Raman fiber probe; and the output end of the Raman fiber probe is connected with the Raman spectrometer and the detector thereof.
In this embodiment, the raman fiber probe is Y-shaped, and includes a first branch and a second branch, where the first branch includes an excitation fiber, the second branch includes a plurality of collection fibers, and a beam of the middle portion of the excitation fiber and a beam of the middle portion of the collection fibers are a beam combining fiber. The optical fiber probe can conveniently enter a human body cavity through a detection channel of the fiber endoscope to carry out Raman spectrum measurement on tissues in the human body cavity.
In this embodiment, the end face of the beam combining optical fiber at the detection end portion is formed by arranging a plurality of collecting optical fibers circumferentially around the excitation optical fiber, and the detection end portion is fixed by a metal sleeve to ensure the flatness and firmness of the end face of the detection portion.
In this embodiment, an adjustable limiting device is disposed at a position, close to a branch, of a combined optical fiber of the raman optical fiber probe, the limiting device is used for adjusting the length of the optical fiber probe entering a detection channel of a fiber endoscope to limit, the limiting device includes a fixing sleeve and a fixing screw, the fixing sleeve is sleeved on the surface of the raman optical fiber probe, and the fixing screw is vertically disposed on the fixing sleeve and used for fixing the raman optical fiber probe. The limiting device can adjust the length of the optical fiber probe entering the detection channel of the endoscope to limit according to the detection requirement, so that the end surface of the detection end of the optical fiber probe does not contact with mucosal tissues in the cavity of a human body when the optical fiber probe passes through the detection channel to enter the cavity for detection, and medical risks such as bleeding and infection caused by damage to the mucosal tissues in the cavity are avoided; and the optical fiber probe can be fixed through the conical rubber sleeve, so that the deviation of measurement point positions caused by the lateral inclination, the movement and the like of the optical fiber probe in the measurement process is prevented, and the positioning precision of the optical fiber probe to a focus is improved.
In the present embodiment of the present invention, the excitation optical fiber and the collection optical fiber are both wrapped by polymer materials.
In this embodiment, fig. 4 is a schematic diagram of a coating film on a detection end of an optical fiber probe, a coating film is disposed on an optical fiber end surface of the detection end of the raman optical fiber probe, and a low-pass film allowing excitation lights with two wavelengths to pass through is coated on an end surface of the excitation optical fiber, so as to reduce interference on measurement caused by irradiation of non-excitation lights generated by the excitation optical fiber and other optical elements on a tissue; the end face of the collection optical fiber is plated with a high-pass film which is used for cutting off the exciting light and allowing Raman scattered light with larger wavelength to pass through so as to reduce the interference of the exciting light reflected by the tissue and entering the collection optical fiber on the Raman signal of the tissue.
In this embodiment, the output optical fiber of the dual-wavelength laser is connected to the excitation optical fiber of the raman optical fiber probe through a filtering component, so as to alternately output two types of excitation light with different wavelengths; and a collecting optical fiber of the Raman optical fiber probe is connected with the filtering component, and the output light of the Raman optical fiber probe is connected with the Raman spectrometer and the detector thereof through the collecting optical fiber.
In this embodiment, the dual-wavelength laser alternately outputs two types of excitation light with different wavelengths, including 785nm excitation light and 690nm excitation light; the 785nm exciting light is used for detecting the Raman spectrum of the fingerprint area, and the 690nm exciting light is used for detecting the Raman spectrum of the high wavenumber area. The dual-wavelength laser is matched with a light filtering component and control software to realize that the Raman spectrum (200-2000 cm) of a fingerprint area is completed by the same spectrum detector with a smaller area -1 Finger print) and high wavenumber region (2600-3500 cm) -1 , high wavenumber) Raman spectrum, effectively reducing the volume of the system and reducing the design cost.
In the embodiment, the fiber endoscope is a fiber endoscope for different parts in the human body cavity, including nasopharyngoscope, cystoscope, hysteroscope, vocal cord arthroscope, and uretero-nephroscope.
In this embodiment, the white light cold light source is a 300W short arc xenon lamp white light cold light source.
In this embodiment, the data processing and displaying device is a PC.
<xnotran> , , , ; </xnotran> The Raman spectrum detection system has the advantages of wide wave number coverage range, small volume and the like, thereby providing an effective clinical detection tool for nondestructive and rapid diagnosis of living tissues and having important application value.
In this embodiment, the fiber endoscope can be a fiber nasopharyngoscope, the inner diameter of the detection channel is 2.2mm, the outer diameter of the beam combining part of the fiber probe is 1.6mm, when the device is used, the beam combining part of the optical fiber probe enters the nasal cavity through the nasopharyngoscope detection channel and reaches the position near the nasopharyngoscope tissue. The adjustable limiting device is arranged at the position, close to the branch, of the beam combining part of the optical fiber probe, the limiting device and the beam combining part of the optical fiber probe are fixed through fastening screws, when the optical fiber probe extends into the endoscope, the limiting device is close to the upper end opening of the detection channel along with the optical fiber probe, when the limiting device reaches the opening of the detection channel, the limiting device can be embedded into the opening of the detection channel, and the optical fiber probe is limited to continue to move towards the human body cavity.
In this embodiment, the connection relationship between the "Y" shaped fiber probe and the dual-wavelength laser, the detector, and the position limiting device is shown in fig. 2, and fig. 3 is a schematic diagram of the structure of the position limiting device in the fiber probe. <xnotran> 3 3 . </xnotran> The limiting device of the Raman fiber probe 2 comprises a fixing part a sleeve 5 and a fixing screw 4 for fixing the sleeve; the fixing sleeve 5 is made of rubber and is in a conical shape so as to firmly fix the Raman fiber probe 2 and prevent measurement errors caused by the lateral inclination and movement of the fiber probe in the measurement process; the fixing sleeve can move freely on the surface of the Raman fiber probe 2, the length of the Raman fiber probe 2 entering the endoscope 1 is accurately adjusted by matching with the fixing screw 4, and the fixing sleeve is reasonably adjusted according to specific detection conditions so as to meet various testing requirements.
In this embodiment, the raman spectrum detection device for living body intracavity tissue of the fiber endoscope is used to perform raman spectrum test on the living body tissues of normal and nasopharyngeal carcinoma, and the test spectra are shown in fig. 5 and fig. 6. FIG. 5 shows the average Raman spectrum of the nasopharyngeal tissue in the normal area of the fingerprint area and the average Raman spectrum of the nasopharyngeal tissue measured by 785nm laser excitation. To our knowledge, this is the first time to measure the Raman spectrum signal of living nasopharyngeal carcinoma tissue at low wavenumber. By contrast, 851, 943, 1004, 1096, 1124, 1265, 1316, 1450, 1621, and 1660cm appear in normal versus tumor tissue raman spectra -1 (ii) the raman peak of (a); but at the same time, some spectral characteristics of nasopharyngeal carcinoma tissues are also found to be changed obviously compared with normal tissues, for example, the peaks at 851, 943, 1096, 1124 have dropped; and at 1004, 1265, 1316, 1450, 1621 and 1660cm -1 The peak at (a) rises. In addition, the shape of the spectrum of normal tissue and tumor tissue is 1120-1360 cm -1 And 1560-1680cm -1 There is also a significant difference between the intervals. Because the Raman peaks are respectively attributed to specific biochemical substances, the intensity change of the Raman peak position indicates that certain biochemical components in tissues are changed specifically along with the development of nasopharyngeal carcinoma. Such as tryptophan, phenylalanine, beta-alanine the content of protein such as tyrosine and the like is changed, in addition, the structure of some proteins has also been altered. <xnotran> , , , . </xnotran> The remarkable changes of the spectra show that the constructed living Raman system of nasopharyngeal carcinoma can detect the specific changes of nasopharyngeal carcinoma tissues, and is expected to be practicalThe nondestructive living detection of nasopharyngeal carcinoma is carried out. FIG. 6 is the average Raman spectrum of normal tissue and nasopharyngeal carcinoma tissue in the high wavenumber region as measured by 690nm laser excitation. The Raman spectrum signal of the living tissue of the nasopharyngeal darcinoma with high wave number is firstly measured, and 2854 cm, 2940 cm and 3009 cm can be obtained from the Raman spectrum signal of the normal tissue and the nasopharyngeal darcinoma tissue in the high wave number range -1 And 3067 cm -1 Equal raman peaks, and the difference between the two: compared with normal tissue, the spectrum of nasopharyngeal carcinoma tissue is 2854 cm and 2940 cm -1 The peak at (a) rises.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (3)
1. A Raman spectrum detection device based on a fiber endoscope is characterized in that: the Raman spectrometer comprises a dual-wavelength laser, a Raman fiber probe, a fiber endoscope, a white light cold light source, a camera device, a Raman spectrometer and a data processing and display device; the white light cold light source is connected with an optical interface of the fiber endoscope, the camera device is arranged at the upper part of the fiber endoscope and is used for collecting images in the fiber endoscope, and the output end of the camera device is connected with the data processing and displaying device and is used for displaying the images in the fiber endoscope; the output end of the dual-wavelength laser is connected with the input end of the Raman fiber probe; the output end of the Raman fiber probe is connected with the Raman spectrometer and the detector thereof; the Raman fiber probe is Y-shaped and comprises a first branch and a second branch, the first branch comprises an excitation fiber, the second branch comprises a plurality of collecting fibers, and the middle part of the excitation fiber and the middle parts of the plurality of collecting fibers are combined into a combined fiber; the beam combining optical fiber is arranged on the end face of the detection end part, a plurality of collecting optical fibers are arranged around the excitation optical fiber in a circumferential mode, and the detection end part is fixed through a metal sleeve; the excitation optical fiber and the collection optical fiber are both wrapped by polymer materials; an adjustable limiting device is arranged at a position, close to a branch, of a combined optical fiber of the Raman optical fiber probe, the limiting device is used for adjusting the length of the optical fiber probe entering a detection channel of a fiber endoscope to limit, the limiting device comprises a fixing sleeve and a fixing screw, the fixing sleeve is sleeved on the surface of the Raman optical fiber probe, and the fixing screw is vertically arranged on the fixing sleeve and used for fixing the Raman optical fiber probe;
the end face of the optical fiber of the detection end part of the Raman optical fiber probe is provided with a coating film, the end face of the excitation optical fiber is coated with a low-pass film which allows excitation light with two wavelengths to pass through, and the end face of the collection optical fiber is coated with a high-pass film which is used for cutting off the excitation light and allows Raman scattered light with larger wavelength to pass through;
the output optical fiber of the dual-wavelength laser is connected with the excitation optical fiber of the Raman optical fiber probe through a filtering component and is used for alternately outputting excitation light with two different wavelengths; the collection optical fiber of the Raman optical fiber probe is connected with the filtering component, and the output light of the Raman optical fiber probe is connected with the Raman spectrometer and the detector thereof through the collection optical fiber;
the dual-wavelength laser alternately outputs two types of exciting light with different wavelengths, wherein the two types of exciting light comprise 785nm exciting light and 690nm exciting light; the 785nm exciting light is used for detecting the Raman spectrum of the fingerprint area, and the 690nm exciting light is used for detecting the Raman spectrum of the high wavenumber area.
2. A fiber endoscope-based raman spectroscopy detection apparatus according to claim 1, wherein: the fiberscope is a fiberscope meeting different parts in a detection channel and comprises a nasopharyngoscope, a cystoscope, a hysteroscope, a vocal cord arthroscope and a ureter-nephroscope.
3. A fiber endoscope-based raman spectroscopy detection apparatus according to claim 1, wherein: the white light cold light source is a 300W short arc xenon lamp white light cold light source.
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| CN201610375997.8A CN105997000B (en) | 2016-05-30 | 2016-05-30 | Raman spectrum detection device based on fiber endoscope and implementation method thereof |
| PCT/CN2016/085988 WO2017206201A1 (en) | 2016-05-30 | 2016-06-16 | Fiberscope-based raman spectrum inspection device and method for implementing same |
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| CN108066001B (en) * | 2016-11-10 | 2020-11-06 | 萧慕东 | Working mirror with induction probe |
| CN106923781A (en) * | 2017-03-28 | 2017-07-07 | 戎创前沿科技(北京)有限公司 | A kind of Raman Gastroscope Diagnosis instrument |
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