CN105974765A - Portable digital holographic microscopy - Google Patents
Portable digital holographic microscopy Download PDFInfo
- Publication number
- CN105974765A CN105974765A CN201610289338.2A CN201610289338A CN105974765A CN 105974765 A CN105974765 A CN 105974765A CN 201610289338 A CN201610289338 A CN 201610289338A CN 105974765 A CN105974765 A CN 105974765A
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- China
- Prior art keywords
- digital holographic
- pallet
- portable digital
- light source
- base
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000009647 digital holographic microscopy Methods 0.000 title abstract description 8
- 238000005070 sampling Methods 0.000 claims abstract description 10
- 238000007639 printing Methods 0.000 claims description 9
- 239000011324 bead Substances 0.000 claims description 7
- 239000007769 metal material Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 229920002521 macromolecule Polymers 0.000 claims description 2
- 239000011347 resin Substances 0.000 claims description 2
- 229920005989 resin Polymers 0.000 claims description 2
- 238000001093 holography Methods 0.000 abstract description 5
- 238000005259 measurement Methods 0.000 abstract description 5
- 230000003287 optical effect Effects 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000010586 diagram Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 230000005540 biological transmission Effects 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 241000239290 Araneae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 229910044991 metal oxide Inorganic materials 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000000399 optical microscopy Methods 0.000 description 1
- 230000005622 photoelectricity Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/0005—Adaptation of holography to specific applications
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/0005—Adaptation of holography to specific applications
- G03H2001/0033—Adaptation of holography to specific applications in hologrammetry for measuring or analysing
- G03H2001/0038—Adaptation of holography to specific applications in hologrammetry for measuring or analysing analogue or digital holobjects
-
- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/0005—Adaptation of holography to specific applications
- G03H2001/005—Adaptation of holography to specific applications in microscopy, e.g. digital holographic microscope [DHM]
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Holo Graphy (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The invention discloses a digital holographic microscopy and aims at providing a portable digital holographic microscopy. The device comprises a light source, a hole board with light transmitting pinholes, and an integrated circuit board loaded with a CMOS sensor or a CCD sensor, wherein the light source, the hole board and the integrated circuit board are arranged sequentially along the optical path; a protection shell comprises a top cover, a base and a shell, and the top cover and the base are connected via the shell; the light source and the hole board are fixed at the inner side of the top cover; the integrated circuit board is fixed at the inner side of the base; a tray bayonet is arranged in the shell for enabling a tray loaded with a sampling board to be inserted. According to the digital holographic microscopy disclosed by the invention, no lens is needed; the digital holography is used for realizing recording of a microscopic image in small space, carrying is facilitated, the structure is simple, the cost is low, holographic microscopic effects are obvious, and promotion and application of the digital holographic microscopic measurement technology in engineering practice are facilitated.
Description
Technical field
The present invention relates to a kind of Digital holographic microscopy device, particularly relate to one and pass based on LED illumination and CMOS
The portable digital holographic microphotography device of sensor image record.
Background technology
Digital holographic microscopy is a kind of novel microscopy having merged digital hologram and optical microscopy, by using photoelectricity
Coupled apparatus (CCD) or complementary metal oxide semiconductors (CMOS) (CMOS) carry out discretization numeral to optical hologram
Record, and utilize computer that digital hologram carries out digital reproduction, thus quantitatively obtain amplitude and the phase of original object light field
Position distribution, has and sample can carry out the features such as nondestructive measurement and accuracy of detection are high, measuring speed is fast.Numeral
Micro-holographic technique be the fields such as microstructure appearance measurement, imaging biological cells, micro-fluidic measurement provide a kind of noncontact,
Current events and the means of measurement of full field.
The digital micro-holography of common laboratory is broadly divided into coaxial and off-axis two kinds, and the main distinction is: axis light
Lu Zhong, Object light wave and reference light wave are all propagated along optical axis direction, and both angles are zero；In off-axis light path, pass through light splitting
Object light is separated by prism with reference light, and the two direction of propagation exists an angle.But either which kind of Digital holographic microscopy
Technology, is required for the complicated light path utilizing laboratory large-scale laser and being made up of spectroscope, lens, displacement platform etc.,
Study in being only useful in laboratory.Further, use for laboratory Digital holographic microscopy device structure is complicated, instrument precision,
Cost is high, is unfavorable for the promotion and application in engineering reality of this technology.
To this end, portable digital holographic microphotography device of the present invention utilizes projection-type principle of holography, use LED lamp bead
As light source, the integrated chip of CMOS is image recorder, it is not necessary to complex optical path and large-scale laser, simple in construction,
It is easy to carry, low cost, just with popularization and actual application, good holographic microphotography effect can be realized.
Summary of the invention
The technical problem to be solved in the present invention is, for the deficiencies in the prior art, it is provided that a kind of portable digital holographic microphotography
Device.This device is without lens complex optical path and large-scale laser, simple in construction, with low cost, it is simple in engineering reality
Carry and apply.
For solving technical problem, the technical solution used in the present invention is:
There is provided a kind of portable digital holographic microphotography device, including the light source being sequentially arranged along light path, with printing opacity pin hole
Orifice plate and the surface-mounted integrated circuit being loaded with cmos sensor or ccd sensor；It is characterized in that, also a protection
Shell, containment vessel includes top cover, base and housing, and top cover is connected by housing with base；Described light source and orifice plate are fixed on top
Inside lid, surface-mounted integrated circuit is fixed on inside base；Housing is offered pallet bayonet socket, is used for inserting and can load sampling plate
Pallet.
In the present invention, described light source and orifice plate are fixed on a support, and support is assemblied in the table inside top cover by screw
On face.
In the present invention, described base is provided with the fixing seat in holding wire passage, screwed hole or disposed thread hole.
In the present invention, described pallet is the frame-type structure of hollow, and frame-type structure is provided with sampling plate bayonet socket；One end of pallet
If drawing handle, pallet both sides respectively set an orbit of guide rail；At the pallet bayonet socket of case inside, it is provided with two and light path
Vertical fixing guide rail；Article two, keeping parallelism between fixing guide rail, its shape cooperates with the orbit of guide rail of pallet.
In the present invention, described light source is LED lamp bead or laser diode (can also be that other can send stable monochrome
The portable light source of light, the color of sent light can be red, green or the light of other colors, and power supply can be 3V
Button cell), light source is γ >=5cm with the distance of pallet.
In the present invention, described orifice plate is lighttight dark solid macromolecule copolymer or the orifice plate of metal material；Printing opacity pin
The aperture in hole is
In the present invention, the pixel quantity of described cmos sensor or ccd sensor is α >=3,000,000, each pixel
Size be 1 μm≤β≤8 μm.
In the present invention, cuboid that described containment vessel is made up of dark resin material or metal material or cylindrical structure
(or structure that protectiveness is good and light-proofness is good of other shape, material).
In the present invention, the quantity of described pallet bayonet socket is σ >=1.
Inventive principle describes:
The light that light source sends forms sphere light by printing opacity pin hole, and a sphere light part is irradiated to be positioned at sampling plate bayonet socket
On sample, through the propagation law formation light wave of the light such as projection and refraction, another part is not irradiated to the light of sample
Shine directly on cmos sensor as reference light.Object light wave and reference light cmos sensor on occur dry
Relate to, after the sample holograms picture of formation is by cmos sensor record, by surface-mounted integrated circuit, signal processed,
It is converted into digital signal.Digital signal leads to USB transmission line and is transferred to terminal and further processes, and then
Hologram image to sample.USB transmission line can either transmit image information, can be again the integrated chip power supply of CMOS.
Terminal, by the further process to hologram image, i.e. obtains the particle diameter of sample, concentration segment information.
Compared with prior art, the invention has the beneficial effects as follows:
The present invention relates to portable digital holographic microphotography device, this device, without camera lens, utilizes Digital Holography real
Micro-image record in present little space, it is simple to carry, simple in construction, with low cost, holographic microphotography effect is notable,
Be conducive to applications of digital micro-holography popularization in engineering practice and application.
Accompanying drawing explanation
Fig. 1 portable digital holographic microphotography device index path；
Fig. 2 portable digital holographic microphotography unit protection shell structure schematic diagram；
Fig. 3 containment vessel of the present invention STRUCTURE DECOMPOSITION schematic diagram；
Fig. 4 inventive shell structural representation；
Fig. 5 support holder structure of the present invention schematic diagram.
1.LED lamp bead in figure；2. orifice plate；3. sample area；4.CMOS sensor；5. surface-mounted integrated circuit；6. the end
Seat；7. fix seat；8. holding wire passage；9. pallet bayonet socket；10. housing；11. top covers；12. supports；13. is solid
Determine screw；14. fix guide rail；15. screwed holes；16. drawing handles；17. orbit of guide rail；18. pallets；19. adopt
Model bayonet socket；20. connecting screw holes.
Detailed description of the invention
The most further describe in detail:
Fig. 1 gives a kind of portable digital holographic microphotography device index path, including LED lamp bead 1, printing opacity pin hole 2,
Sample area 3, cmos sensor and surface-mounted integrated circuit 5.LED lamp bead 1 passes through printing opacity pin as light source, the light sent
Hole 2 forms sphere light, and a sphere light part is irradiated on the sample in sample area 3, through the biography of the light such as projection and refraction
Broadcasting rule formation light wave, another part is not irradiated to the light of sample and shines directly into CMOS biography as reference light
On sensor 4.Object light wave and reference light interfere on cmos sensor 4, form the hologram image record of sample
In cmos sensor 4, and carry out signal processing be converted into digital signal, digital signal by surface-mounted integrated circuit 5
It is transferred to terminal by USB transmission line further process, and then obtains the hologram image of described sample.
Terminal, by the further process to described hologram image, obtains the particle diameter of described sample, concentration segment information.
Fig. 2 is portable digital holographic microphotography unit protection shell structure schematic diagram.Containment vessel is by base 6, housing 10 and top
Lid 11 composition.Wherein, base 6 is provided with fixing seat 7, holding wire passage 8 and screwed hole 15.USB transmission line leads to
Cross holding wire passage 8 to derive, be connected to terminal and carry out signal processing.Will collection by screwed hole 15 with screw
Circuit board 5 is become to be fixed on the inner side of base 6.During assembling, by screw or screw rod through the through hole on base 6, by with
The cooperation of the connecting screw hole 20 being located on housing 10, is assembled together base 6 with housing 10.Fixing seat 7 has
Screwed hole, by being fixed on outside support whole device (just as slr camera with screw or coordinating of stud during use
Being fixed by screws on spider like that, fixing seat 7 plays the fixing effect connecting whole device).
Fig. 3 gives containment vessel STRUCTURE DECOMPOSITION schematic diagram of the present invention, and support 12 is used for fixing LED lamp bead 1 and orifice plate 2
(setting printing opacity pin hole on orifice plate 2), is assemblied in support 12 on the inner surface of top cover 11 by screw, it is achieved light source
Fixing.
Fig. 4 gives inventive shell structural representation.Offer two pallet bayonet sockets 9 on the housing 10, be used for inserting energy
Load the pallet 18 of sampling plate.Fig. 5 gives support holder structure schematic diagram of the present invention.The pallet 18 frame-type structure in hollow,
Frame-type structure is provided with sampling plate bayonet socket 19；Sampling plate bayonet socket 19 is used for fixing the sampling plate loading sample, the frame of hollow
Type structure makes sample area 3 can pass through Object light wave and reference light.One end of pallet 18 sets drawing handle 16, pallet 18
Both sides respectively set an orbit of guide rail 17；On the housing 10, it is provided with two in two between the pallet bayonet socket 9 offered
The fixing guide rail 14 that bar is vertical with light path；Article two, the guide rail of keeping parallelism between fixing guide rail 14, its shape and pallet 18
Track 19 cooperates.In the present invention, the length that can enter pallet bayonet socket 9 by changing pallet 18 changes sample
The position in district 3, to ensure preferably to measure visual field.Be additionally provided with fixing threaded hole 13 on housing 10, be used for loading screw or
Screw rod realizes fixing to withstand pallet 18, it is ensured that improving and hologram image clear of sample.
The above is the preferred embodiment of the present invention, it is noted that come for those skilled in the art
Saying, under the premise without departing from the principles of the invention, it is also possible to make some improvements and modifications, these improvements and modifications also regard
For protection scope of the present invention.
Claims (9)
1. a portable digital holographic microphotography device, including the light source being sequentially arranged along light path, with printing opacity pin hole
Orifice plate and the surface-mounted integrated circuit being loaded with cmos sensor or ccd sensor；It is characterized in that, also a protection
Shell, containment vessel includes top cover, base and housing, and top cover is connected by housing with base；Described light source and orifice plate are fixed on top
Inside lid, surface-mounted integrated circuit is fixed on inside base；Housing is offered pallet bayonet socket, is used for inserting and can load sampling plate
Pallet.
Portable digital holographic microphotography device the most according to claim 1, it is characterised in that described light source and hole
Plate is fixed on a support, and support is assemblied on the surface inside top cover by screw.
Portable digital holographic microphotography device the most according to claim 1, it is characterised in that set on described base
There is the fixing seat in holding wire passage, screwed hole and disposed thread hole.
Portable digital holographic microphotography device the most according to claim 1, it is characterised in that described pallet in
Empty frame-type structure, frame-type structure is provided with sampling plate bayonet socket；One end of pallet sets drawing handle, and pallet both sides respectively set one
Bar orbit of guide rail；At the pallet bayonet socket of case inside, it is provided with two fixing guide rails vertical with light path；Article two, fix and lead
Keeping parallelism between rail, its shape cooperates with the orbit of guide rail of pallet.
Portable digital holographic microphotography device the most according to claim 1, it is characterised in that described light source is LED
Lamp bead or laser diode, light source is γ >=5cm with the distance of pallet.
Portable digital holographic microphotography device the most according to claim 1, it is characterised in that described orifice plate is not
The dark solid macromolecule copolymer of printing opacity or the orifice plate of metal material；The aperture of printing opacity pin hole is
Portable digital holographic microphotography device the most according to claim 1, it is characterised in that described CMOS passes
The pixel quantity of sensor or ccd sensor is α >=3,000,000, and the size of each pixel is 1 μm≤β≤8 μm.
Portable digital holographic microphotography device the most according to claim 1, it is characterised in that described containment vessel is
The cuboid being made up of dark resin material or metal material or cylindrical structure.
Portable digital holographic microphotography device the most according to claim 1, it is characterised in that described pallet bayonet socket
Quantity be σ >=1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610289338.2A CN105974765A (en) | 2016-05-02 | 2016-05-02 | Portable digital holographic microscopy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610289338.2A CN105974765A (en) | 2016-05-02 | 2016-05-02 | Portable digital holographic microscopy |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105974765A true CN105974765A (en) | 2016-09-28 |
Family
ID=56994411
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610289338.2A Pending CN105974765A (en) | 2016-05-02 | 2016-05-02 | Portable digital holographic microscopy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105974765A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018068112A1 (en) * | 2016-10-11 | 2018-04-19 | Guern Tecnologia Da Informação Ltda - Me | Portable diagnosis test and use thereof |
| CN110487221A (en) * | 2019-08-20 | 2019-11-22 | 大连海事大学 | Hologram fringe central bright spot area and the micro- plastics detection device of dimension ratio and method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201974574U (en) * | 2011-03-09 | 2011-09-14 | 北京工业大学 | Inverted digital holographic microscope |
| CN202351504U (en) * | 2011-12-14 | 2012-07-25 | 南京大学 | Portable digital microscope based on ultrahigh-resolution imaging chip |
| CN102660457A (en) * | 2012-04-17 | 2012-09-12 | 南昌航空大学 | Device and method for analyzing and counting blood cells by lensless holographic diffraction imaging |
| CN103149827A (en) * | 2013-02-28 | 2013-06-12 | 山东大学 | Method for eliminating single-beam coaxial digital holography direct current terms and conjugate images |
| CN104111257A (en) * | 2014-07-24 | 2014-10-22 | 江苏大学 | System and method for phase micro-interference imaging based on common-optical path system |
-
2016
- 2016-05-02 CN CN201610289338.2A patent/CN105974765A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201974574U (en) * | 2011-03-09 | 2011-09-14 | 北京工业大学 | Inverted digital holographic microscope |
| CN202351504U (en) * | 2011-12-14 | 2012-07-25 | 南京大学 | Portable digital microscope based on ultrahigh-resolution imaging chip |
| CN102660457A (en) * | 2012-04-17 | 2012-09-12 | 南昌航空大学 | Device and method for analyzing and counting blood cells by lensless holographic diffraction imaging |
| CN103149827A (en) * | 2013-02-28 | 2013-06-12 | 山东大学 | Method for eliminating single-beam coaxial digital holography direct current terms and conjugate images |
| CN104111257A (en) * | 2014-07-24 | 2014-10-22 | 江苏大学 | System and method for phase micro-interference imaging based on common-optical path system |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018068112A1 (en) * | 2016-10-11 | 2018-04-19 | Guern Tecnologia Da Informação Ltda - Me | Portable diagnosis test and use thereof |
| CN110487221A (en) * | 2019-08-20 | 2019-11-22 | 大连海事大学 | Hologram fringe central bright spot area and the micro- plastics detection device of dimension ratio and method |
| CN110487221B (en) * | 2019-08-20 | 2021-05-25 | 大连海事大学 | Micro-plastic detection device and method for area-to-size ratio of central bright spot of holographic stripe |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160928 |
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