CN105954270A - Solution for evaluating glucose metabolism based on sialology method and evaluation method thereof - Google Patents
Solution for evaluating glucose metabolism based on sialology method and evaluation method thereof Download PDFInfo
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- CN105954270A CN105954270A CN201610274043.8A CN201610274043A CN105954270A CN 105954270 A CN105954270 A CN 105954270A CN 201610274043 A CN201610274043 A CN 201610274043A CN 105954270 A CN105954270 A CN 105954270A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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Abstract
The invention provides a solution for evaluating the glucose metabolism based on the sialology method and an evaluation method thereof. The solution comprises the following components: 60-150 mM of pH 7.6 Tris-HCl, 0.12-0.4% of blue chromogenic substances; 1200-2500 IU/L of glucose oxidase; 120-250 IU/L of horse radish peroxidase; and 600-1500 IU/L of antioxidant. The evaluation method comprises the steps of 1) collecting the saliva; 2) mixing the saliva with the above solution; 3) determining the level of glucose metabolism of an organism according to the color depth of the mixed solution of the saliva and the solution. According to the technical scheme of the invention, the level of glucose metabolism is evaluated by adopting the above solution that can change in color depth after reacting with the saliva. In this way, the inconvenience caused by the detection of the blood glucose can be solved. Meanwhile, the evaluation method is visual, convenient, fast, high in efficiency and high in accuracy. At the same time, the antioxidant is added into the above solution, so that the storage life of the solution is prolonged. Moreover, the waste is avoided and the cost is saved.
Description
Technical field
The present invention relates to biology, veterinary and medical research technical field, particularly relate to a kind of by sialology method
The solution of assessment glucose metabolism and appraisal procedure.
Background technology
Along with growth in the living standard, crowd's ratio of mankind's having diabetes is more and more higher, the glucose of detection body
Metaboilic level has great importance for prevention diabetes.
Existing glucose sensing approach includes the detection method of chemically based glucose sensor formerly and based on electricity
Learning the detection method of glucose sensor, both of which cannot provide a kind of more convenient and detection side of low cost
Method.Except both the above method, also having and use blood-sugar detecting instrument detection, these costs are high, and operate requirement height.
And the patent of invention of Patent No. 201480004302.4 discloses Saliva Glucose monitoring system, the method is logical
Crossing the glucose content in glucose sensor detection saliva, therefore cost is high, and operation requires height.
The patent of invention of Patent No. 201210579953.9 discloses a kind of glucose detection test paper, is that this reagent paper enters
The method of row glucose detection.When dripping glucose solution on reagent paper, glucose oxidase glucose also produced
Hydrogen oxide, is oxidized into coloured product by colourless o-dianisidine under the catalytic action of peroxidase, so that
Test paper color development, carries out the detection by quantitative of glucose by measuring reagent paper shade.The preparation process of the application reagent paper also compares
Complexity, therefore cannot provide a kind of more convenient, detection method of low cost.
In existing document, except the glucose metabolism level by blood sampling detection human body, now also by saliva
Assessment glucose metabolism level.Many researchs have been proven that between blood-glucose and Saliva Glucose level and there is association,
The most directly application Saliva Glucose measurements shows that individual health status is feasible and apparently can be real in theory
Existing, and have body non-invasive by the glucose metabolism of the horizontal Indirect evaluation body of glucose in accurately test saliva
Feature.And detect blood sugar level and need by numerous and diverse blood sampling procedure, and blood sampling itself is also a kind of injury to body.
Existing be all the reaction solution hybrid reaction by saliva and preparation by saliva assessment glucose metabolism level after
Observe shade assessment glucose metabolism level, and the chromogen substance now used is all based on yellow, sees yellow
Depth assessment glucose metabolism, but owing to saliva being contained the patient of part yellow substance, especially oral hemorrhage, yellow
Matter is more, thus can the accuracy of impact evaluation result；And the storage life of existing reaction solution is low.
Summary of the invention
It is an object of the invention to existing glucose detection cannot provide a kind of more convenient, low cost accuracy
High and the detection method of reaction solution length storage life and a kind of molten by the assessment glucose metabolism of sialology method is proposed
Liquid and appraisal procedure.
For reaching this purpose, the present invention by the following technical solutions:
A kind of solution by the assessment glucose metabolism of sialology method, its preparation includes following components:
Tris-HCl pH 7.6:60-150mM；
Chromogen substance: 0.12-0.4%；
Glucoseoxidase: 1200-2500IU/L；
Horseradish peroxidase: 120-250IU/L；
Antioxidant: 600-1500IU/L.
Preferably, described blue toner material is the mixed of 2,4,6-tri-iodo-3-hydroxy benzoic acid and 4-AA
Compound.
Preferably, described 2,4,6-tri-iodo-3-hydroxy benzoic acid: 0.06-0.2%；Described 4-AA:
0.06-0.2%.
Preferably, described antioxidant is glutathion.
Preferably, it is formulated by following components:
Tris-HCl pH 7.6:100mM；
2,4,6-tri-iodo-3-hydroxy benzoic acid: 0.1%；
4-AA: 0.1%；
Glucoseoxidase: 2000IU/L；
Horseradish peroxidase: 200IU/L；
Glutathion: 1000IU/L.
A kind of appraisal procedure by sialology method assessment glucose metabolism level described above, it includes following step
Rapid:
1), saliva is gathered；
2), saliva is mixed with described solution；
3) shade obtaining mixed liquor according to saliva after, mixing with solution determines the glucose metabolism level of body；
Blueness is more deeply felt and is shown that glucose content is the highest；Blueness the most shallow expression glucose content is the lowest.
Preferably, the mixed volume of described saliva and described solution is than for 1:15.
Preferably, described saliva and described solution are placed in hybrid reaction in assay flask.
The present invention by use a kind of with ptyaloreaction after produce the solution of shade and assess the metabolic water of glucose
Flat, solve the inconvenience that detection blood glucose brings；And this appraisal procedure is directly perceived, convenient and swift, efficiency is high, accuracy is high；
Add antioxidant the most in this solution to reach to extend the storage life of this solution, it is to avoid waste, cost-effective.
Detailed description of the invention
Further illustrate technical scheme below and by detailed description of the invention.
Illustrate at this, owing to oral cavity typically not having the existence of blue material, so the application selects blue toner thing
Matter, would not be affected by other colors in saliva, it is ensured that the accuracy of testing result；Add antioxygen in the solution simultaneously
Agent, extends the storage life of solution, it is to avoid waste, cost-effective.Certainly the application is molten with this not only by saliva
After liquid hybrid reaction produce shade judge glucose metabolism level, it is also possible to by spectrophotometric determination saliva and this
After solution mixing, the optical density value of mixed liquor is as the absolute value (mM level) of body pol, but this method is complicated,
Do not observe shade more directly perceived, and convenient and swift efficiency is high.
And reaction principle after the mixing of saliva and this solution: the glucose contained in saliva is in the effect of glucoseoxidase
Lower generation hydrogen peroxide, hydrogen peroxide produces nascent oxygen under the effect of horseradish peroxidase, and this nascent oxygen is having
In the case of 4-AA exists, cause colourless 2,4,6-tri-iodo-3-hydroxy benzoic acid become blue, so that whole
Individual mixed liquor becomes blue.Thus by blue depth assessment glucose metabolism level；Blueness is more deeply felt and is shown that glucose content is more
High；Blueness the most shallow expression glucose content is the lowest.
Embodiment 1
A kind of solution by the assessment glucose metabolism of sialology method, it is formulated by following components:
Tris-HCl pH 7.6:80mM；
2,4,6-tri-iodo-3-hydroxy benzoic acid: 0.07%；
4-AA: 0.07%；
Glucoseoxidase: 1800IU/L；
Horseradish peroxidase: 220IU/L；
Glutathion: 1100IU/L.
Using the method that this solution assesses glucose metabolism level, it comprises the following steps:
1) saliva of ten people, is gathered respectively；
2), the saliva of ten people is mixed with described solution respectively；The mixed volume ratio of wherein said saliva and described solution
For 1:15；
3) shade obtaining mixed liquor according to saliva after, mixing with solution determines the glucose metabolism level of body；
Blueness is more deeply felt and is shown that glucose content is the highest；Blueness the most shallow expression glucose content is the lowest.As shown in table 1, ten human glocose generation
Thanking to result, sequence number 1-10 represents 10 people of extraction respectively, and wherein color is from being deeply divided into 5 grades to shallow, and dark blue is 1 grade, and azure is 2
Level, sky blue 3 grades, light blue is 4 grades, close to colourless be 5 grades.
Table 10 human glocose Metabolic products
| Sequence number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Shade | 3 grades | 2 grades | 5 grades | 1 grade | 2 grades | 4 grades | 3 grades | 1 grade | 2 grades | 5 grades |
As known from Table 1, the glucose metabolism level of No. 3 and No. 10 is in normally, and glucose content is relatively low；And No. 4 and No. 8
Glucose metabolism level condition the most pessimistic, glucose content is the highest, belongs to the crowd of having diabetes；No. 2, No. 5
Glucose metabolism level condition with No. 9 is pessimistic, and glucose content is the most of a relatively high, is in diabetes edge；No. 1 and No. 7
Glucose metabolism level is the most better compared with No. 2, but is also at diabetes edge；The glucose content of No. 6 is in acceptable model
Enclose.
Below only just can assess glucose metabolism level by shade, method is simple, and result viewing is directly perceived, fast,
Efficiency is high；The mankind focusing on self glucose metabolism at ordinary times and plays preferable facilitation, prevention diabetes play the most in advance
Preferably preventive effect.
Comparative example 1
A kind of reagent paper utilizing glucose detection carries out the method for the detection of concentration of glucose, and preparation process includes:
1) glucoseoxidase of certain mass, peroxidase are dissolved in buffer and obtain composite reagent solution A；
This composite reagent solution A being sufficiently mixed with glycerol, wherein solution is 10:90-30:70 with the volume ratio of glycerol；By this mixing
Liquid, as the internal phase solution of co-axial electrospinning, joins in microsyringe；
2) the o-dianisidine of certain mass is dissolved in organic solvent, and certain mass high molecular polymer is dissolved in
Fully dissolving in this organic solvent, the clear solution obtained, as foreign minister's electrospinning liquid of co-axial electrospinning, joins another
In one microsyringe.Wherein foreign minister's electrospinning liquid is dissolved in N, N-methylacetamide and (includes the o-di-Fructus Foeniculi of 5-10mg/ml
Amine) percentage by weight be 10～the polyurethane solutions of 30%, be dissolved in DMAC N,N' dimethyl acetamide and (include the o-of 5-10mg/ml
Dianisidine) percentage by weight be 10～the Kynoar solution of 30%, be dissolved in chloroform and (include the o-of 5-10mg/ml
Dianisidine) percentage by weight be 10～the PLA solution of 30% or be dissolved in DMAC N,N' dimethyl acetamide and (include 5-
The o-dianisidine of 10mg/ml) styrene maleic anhydride copolymer solution；
3) inside and outside phase electrospinning liquid is connected to respectively the inlet of coaxial electrically spun spinning head, by spinning head by pipeline
Liquid outlet is connected with the positive pole of high voltage power supply, is connected to the negative pole of high voltage power supply as receiving plate with aluminium-foil paper or stainless (steel) wire；
4) utilize two syringe pumps to control the flow velocity of inside and outside phase electrospinning liquid respectively, carry out coaxial electrostatic spinning, receive plate
On obtain the polymer nanofiber with hollow structure, wherein the composite reagent A for glucose detection is existed by embedding in situ
In the cavity of this hollow Nano fiber in use, the o-dianisidine of developer is then evenly distributed in the shell of doughnut, from
And prepare the reagent paper for glucose detection；
5) by above-mentioned steps 4) in the prepared reagent paper card punch for glucose detection be uniformly cut into diameter
Diaphragm for about 0.5cm size；Prepare the glucose solution of a series of concentration known, take 10-30 μ l glucose with pipettor
Solution drips on the diaphragm that cuts, observes the change of diaphragm color after about 30s, or optical density instrument reads diaphragm at 440nm
The reflectance at place, draws the working curve between known glucose concentrations and reflectance, or using the diaphragm after colour developing as mark
Quasi-colorimetric card；The diaphragm cut drips the glucose solution of 10-30 μ l unknown concentration, after about 30s, observes diaphragm face
Complexion changed, or optical density instrument reading diaphragm reflectance at 440nm, can calculate the Fructus Vitis viniferae in sample according to working curve
Sugar concentration, it is also possible to by compareing with standard color comparison card, the concentration of glucose in rough estimate sample.
The method needs to prepare reagent paper, and the method preparing reagent paper is more complicated, causes whole detection method to become multiple
Miscellaneous, therefore a kind of more convenient, detection method of low cost cannot be provided.
Comparative example 2
A kind of solution by the assessment glucose metabolism of sialology method, it is formulated by following components:
Tris-HCl pH 7.6:80mM；
4-AA: 0.07%；
Glucoseoxidase: 1800IU/L；
Horseradish peroxidase: 220IU/L.
The method using the solution assessment glucose metabolism level of this comparative example 2, its method and the method for embodiment 1
Unanimously, this reaction principle is with the reaction principle of the application, and the color simply produced after hybrid reaction is yellow, and yellow is more deeply felt
Show that glucose content is the highest, but stated in the background owing to saliva going out containing part yellow substance, especially oral cavity
The patient of blood, yellow substance is more, thus can the accuracy of impact evaluation result, therefore use yellow chromogen to be estimated Portugal
The glycometabolic result of grape there are differences, and accuracy is low.
Embodiment group 2
A kind of solution by the assessment glucose metabolism of sialology method, it is formulated by following components:
Tris-HCl pH 7.6:100mM；
2,4,6-tri-iodo-3-hydroxy benzoic acid: (addition of according to the form below 2 adds)
4-AA: 0.1%；
Glucoseoxidase: 2000IU/L；
Horseradish peroxidase: 200IU/L；
Glutathion: 1000IU/L.
Using the method that the solution of the present embodiment group assesses glucose metabolism level, it comprises the following steps:
1) saliva of 1 people, is gathered；
2), the solution of saliva with the present embodiment group is mixed；The mixed volume of described saliva and described solution is than for 1:15；
3) shade obtaining mixed liquor according to the solution of saliva with the present embodiment group after, mixing determines the Fructus Vitis viniferae of body
Sugar metabolism levels.As shown in table 2,2,4, the impact on assessment result of the 6-tri-iodo-3-hydroxy benzoic acid addition, wherein color from
Deeply being divided into 5 grades to shallow, dark blue is 1 grade, and azure is 2 grades, sky blue 3 grades, and light blue is 4 grades, close to colourless be 5 grades.
Table 22,4, the impact on assessment result of the 6-tri-iodo-3-hydroxy benzoic acid addition
| Embodiment | Addition/% | The reaction color depth |
| Contrast groups 2-1 | 0.06 | 4 grades |
| Contrast groups 2-2 | 0.08 | 3 grades |
| Contrast groups 2-3 | 0.1 | 2 grades |
| Contrast groups 2-4 | 0.12 | 2 grades |
| Contrast groups 2-5 | 0.14 | 2 grades |
| Contrast groups 2-6 | 0.15 | 2 grades |
| Contrast groups 2-7 | 0.17 | 2 grades |
| Contrast groups 2-8 | 0.18 | 2 grades |
| Contrast groups 2-9 | 0.2 | 2 grades |
As shown in Table 2, when 2,4, the addition of 6-tri-iodo-3-hydroxy benzoic acid more than 0.1% after, color with addition is
When 0.1% the same, and addition less than 0.1% time, shade is inconsistent.Thus draw assessment glucose metabolism water
Flat accuracy is higher, and in the case of considering cost, need 2,4, the addition of 6-tri-iodo-3-hydroxy benzoic acid be
When 0.1%, the accuracy of assessment can improve a lot.
Embodiment group 3
A kind of solution by the assessment glucose metabolism of sialology method, it is formulated by following components:
Tris-HCl pH 7.6:100mM；
2,4,6-tri-iodo-3-hydroxy benzoic acid: 0.1%
4-AA: (addition of according to the form below 3 adds)
Glucoseoxidase: 2000IU/L；
Horseradish peroxidase: 200IU/L；
Glutathion: 1000IU/L.
Using the method that the solution of the present embodiment group assesses glucose metabolism level, it comprises the following steps:
1) saliva of 1 people, is gathered；
2), the solution of saliva with the present embodiment group is mixed；Described saliva is 1 microlitre with the mixed proportion of described solution:
15 microlitres；
3) shade obtaining mixed liquor according to the solution of saliva with the present embodiment group after, mixing determines the Fructus Vitis viniferae of body
Sugar metabolism levels.As shown in table 3, the impact on assessment result of the 4-AA addition, wherein color from deep to shallow point
Being 5 grades, dark blue is 1 grade, and azure is 2 grades, sky blue 3 grades, and light blue is 4 grades, close to colourless be 5 grades.
The impact on assessment result of the table 3 4-AA addition
| Embodiment | Addition/% | The reaction color depth |
| Contrast groups 3-1 | 0.06 | 4 grades |
| Contrast groups 3-2 | 0.08 | 3 grades |
| Contrast groups 3-3 | 0.1 | 2 grades |
| Contrast groups 3-4 | 0.12 | 2 grades |
| Contrast groups 3-5 | 0.14 | 2 grades |
| Contrast groups 3-6 | 0.15 | 2 grades |
| Contrast groups 3-7 | 0.17 | 2 grades |
| Contrast groups 3-8 | 0.18 | 2 grades |
| Contrast groups 3-9 | 0.2 | 2 grades |
As shown in Table 3, after the addition of 4-AA is more than 0.1%, color is with when addition is 0.1% one
Sample, and when addition is less than 0.1%, shade is inconsistent.Thus draw the accurate of assessment glucose metabolism level
Property higher, and in the case of considering cost, the accuracy meeting of assessment when to need the addition of 4-AA be 0.1%
Improve a lot.
Embodiment group 4
A kind of solution by the assessment glucose metabolism of sialology method, it is formulated by following components:
Tris-HCl pH 7.6:100mM；
2,4,6-tri-iodo-3-hydroxy benzoic acid: 0.1%
4-AA: 0.1%
Glucoseoxidase: 2000IU/L；
Horseradish peroxidase: 200IU/L；
Antioxidant (antioxidant of according to the form below 4 adds): 1000IU/L.
Being shown in Table 4, the storage life of solution is affected by different antioxidants.
The storage life of solution is affected by the different antioxidant of table 4
| Embodiment | Antioxidant | The storage time |
| Contrast groups 4-1 | BHA (Butylated hydroxyanisole) | 2 weeks |
| Contrast groups 4-2 | BHT (dibenzylatiooluene) | 2 weeks |
| Contrast groups 4-3 | PG (propylgallate) | 1 month |
| Contrast groups 4-4 | TBHQ (tert-butylhydroquinone) | 1 month |
| Contrast groups 4-5 | Tocopherol | 1 month |
| Contrast groups 4-6 | Vitamin C | 1 month |
| Contrast groups 4-7 | Glutathion | More than 2 years |
Owing to 2 in solution, 4,6-tri-iodo-3-hydroxy benzoic acid and 4-AA can be empty in storing process
Oxygen in gas is oxidized to other materials of blueness, therefore to stop 2,4,6-tri-iodo-3-hydroxy benzoic acid and 4-amino peace to be replaced
More oxidized than woods, with the addition of antioxidant in the solution.As known from Table 4, BHA, BHT, PG, TBHQ, tocopherol and and dimension are used
Raw element C was less than one month as antioxidant, the storage time of this solution, and the shortest had two weeks, and used glutathion
The storage life of this solution reaches more than 2 years, it can be seen that using glutathion is best selection as antioxidant.
Embodiment group 5
A kind of solution by the assessment glucose metabolism of sialology method, it is formulated by following components:
Tris-HCl pH 7.6:100mM；
2,4,6-tri-iodo-3-hydroxy benzoic acid: 0.1%
4-AA: 0.1%
Glucoseoxidase: 2000IU/L；
Horseradish peroxidase: 200IU/L；
Glutathion: (addition of according to the form below 5 adds).
Being shown in Table 5, the storage life of solution is affected by glutathion Different adding amount.
The storage life of solution is affected by table 5 glutathion addition
| Embodiment | Glutathion addition/IU/L | The storage time |
| Contrast groups 5-1 | 600 | 1 year half |
| Contrast groups 5-2 | 800 | Close to 2 years |
| Contrast groups 5-3 | 900 | 2 years |
| Contrast groups 5-4 | 1000 | More than 2 years |
| Contrast groups 5-5 | 1100 | As contrast groups 5-4 |
| Contrast groups 5-6 | 1300 | As contrast groups 5-4 |
| Contrast groups 5-7 | 1500 | As contrast groups 5-4 |
As known from Table 5, along with the interpolation of glutathion addition, the storage life of solution is extending, and cost also can add
Add；But after addition is more than 1000IU/L, the storage life of solution with use 1000IU/L storage life be,
Therefore solution storage life problem, Cost Problems to be considered should be considered, so the optimum addition of glutathion is
1000IU/L。
In sum, the accuracy of assessment result should be considered, consider cost again, it follows that passing through of a kind of optimum
The solution of sialology method assessment glucose metabolism, it is formulated by following components:
Tris-HCl pH 7.6:100mM；
2,4,6-tri-iodo-3-hydroxy benzoic acid: 0.1%
4-AA: 0.1%
Glucoseoxidase: 2000IU/L；
Horseradish peroxidase: 200IU/L；
Glutathion: 1000IU/L.
Embodiment group 6
A kind of solution by the assessment glucose metabolism of sialology method, it is formulated by following components:
Tris-HCl pH 7.6:80mM；
2,4,6-tri-iodo-3-hydroxy benzoic acid: 0.07%；
4-AA: 0.07%；
Glucoseoxidase: 1800IU/L；
Horseradish peroxidase: 220IU/L；
Glutathion: 1100IU/L.
Select No. 1 saliva in embodiment 1 as assessment saliva, this saliva is mixed with the solution of the present embodiment；Described
Saliva is shown in Table 6 with the mixed volume ratio of described solution；The shade obtaining mixed liquor according to saliva after mixing with solution determines
The glucose metabolism level of body；Blueness is more deeply felt and is shown that glucose content is the highest；Blueness the most shallow expression glucose content is the lowest.See
Shown in table 6, the impact on assessment result of the mixed proportion of saliva and solution.Color is from being deeply divided into 5 grades to shallow, and dark blue is 1 grade, profound
Indigo plant is 2 grades, sky blue 3 grades, and light blue is 4 grades, close to colourless be 5 grades.
The impact on assessment result of the mixed proportion of table 6 saliva and solution
| Embodiment | Saliva and the mixed proportion (microlitre: microlitre) of solution | Shade |
| Contrast groups 6-1 | 1:6 | 5 grades |
| Contrast groups 6-2 | 1:10 | 4 grades |
| Contrast groups 6-3 | 1:15 | 3 grades |
| Contrast groups 6-4 | 1:16 | 3 grades |
| Contrast groups 6-5 | 1:20 | 3 grades |
As known from Table 6, along with the increase of saliva Yu the mixed proportion of solution, the color of mixed liquor is to change from shallow to deep,
And along with the interpolation of solution addition, cost also can add；But when the ratio of saliva and the mixed proportion of solution is more than 1:
After 15, mixed liquor color is 1:15 is with the mixed proportion of saliva Yu solution, therefore should consider to assess accuracy, also
Cost Problems to be considered, so saliva and the mixing optimal proportion 1:15 of solution.In this ratio, the Fructus Vitis viniferae contained in solution
The glucose contained in carbohydrate oxidase and saliva fully reacts, and the assessment result obtained can be the most accurate.
Illustrating at this, the rank of above color can carry out classification according to shade degree, above simply illustrates
The bright classification being not only limited to above color.
The know-why of the present invention is described above in association with specific embodiment.These describe and are intended merely to explain the present invention's
Principle, and limiting the scope of the invention can not be construed to by any way.Based on explanation herein, the technology of this area
Personnel need not pay performing creative labour can associate other detailed description of the invention of the present invention, and these modes fall within
Within protection scope of the present invention.
Claims (8)
1. the solution by the assessment glucose metabolism of sialology method, it is characterised in that: it is prepared and includes following components:
Tris-HCl pH 7.6:60-150mM；
Chromogen substance: 0.12-0.4%；
Glucoseoxidase: 1200-2500IU/L；
Horseradish peroxidase: 120-250IU/L；
Antioxidant: 600-1500IU/L.
A kind of solution by the assessment glucose metabolism of sialology method the most according to claim 1, it is characterised in that: institute
Stating blue toner material is 2,4,6-tri-iodo-3-hydroxy benzoic acid and the mixture of 4-AA.
A kind of solution by the assessment glucose metabolism of sialology method the most according to claim 2, it is characterised in that: institute
State 2,4,6-tri-iodo-3-hydroxy benzoic acid: 0.06-0.2%；Described 4-AA: 0.06-0.2%.
A kind of solution by the assessment glucose metabolism of sialology method the most according to claim 3, it is characterised in that: institute
Stating antioxidant is glutathion.
A kind of solution by the assessment glucose metabolism of sialology method the most according to claim 4, it is characterised in that: its
Formulated by following components:
Tris-HCl pH 7.6:100mM；
2,4,6-tri-iodo-3-hydroxy benzoic acid: 0.1%；
4-AA: 0.1%；
Glucoseoxidase: 2000IU/L；
Horseradish peroxidase: 200IU/L；
Glutathion: 1000IU/L.
6. a kind of assessment side by sialology method assessment glucose metabolism level as described in claim 1-5 any one
Method, it is characterised in that: it comprises the following steps:
1), saliva is gathered；
2), saliva is mixed with described solution；
3) shade obtaining mixed liquor according to saliva after, mixing with solution determines the glucose metabolism level of body；Blue
More deeply feel and show that glucose content is the highest；Blueness the most shallow expression glucose content is the lowest.
Appraisal procedure the most according to claim 6, it is characterised in that: described saliva with the mixed volume ratio of described solution is
1:15.
Appraisal procedure the most according to claim 6, it is characterised in that: described saliva and described solution are placed in assay flask mixed
Close reaction.
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| CN201610274043.8A Pending CN105954270A (en) | 2016-04-27 | 2016-04-27 | Solution for evaluating glucose metabolism based on sialology method and evaluation method thereof |
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| CN111235208A (en) * | 2020-02-28 | 2020-06-05 | 江苏大学 | Method for evaluating influence of ionic liquid on sugar metabolism |
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