CN105941486B - Agricultural insecticidal composition and application thereof - Google Patents
Agricultural insecticidal composition and application thereof Download PDFInfo
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- CN105941486B CN105941486B CN201610323365.7A CN201610323365A CN105941486B CN 105941486 B CN105941486 B CN 105941486B CN 201610323365 A CN201610323365 A CN 201610323365A CN 105941486 B CN105941486 B CN 105941486B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N2300/00—Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/79—Paecilomyces
Abstract
The invention provides an agricultural insecticidal composition, which comprises the following components in parts by weight: 30-90 parts of pharmaceutical active components, wherein the pharmaceutical active components comprise paecilomyces fumosoroseus and beauveria bassiana in a weight ratio of (0.13-4.4) to (0.25-7.5); 10-70 parts of a drug carrier; 0.1 to 20 parts of an auxiliary agent. The paecilomyces fumosoroseus and the beauveria bassiana in the agricultural insecticidal composition are compounded, so that the obtained agricultural insecticidal composition is efficient and low in toxicity, stable in insecticidal effect, safe to people, livestock and crops, capable of protecting the crops, free of damaging natural enemies, high in safety, good in environmental protection performance, high in control efficiency up to more than 85%, and far higher in insecticidal effect than that of the paecilomyces fumosoroseus or the beauveria bassiana which is sprayed independently.
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly relates to an agricultural insecticidal composition and application thereof in preventing and treating crop diseases.
Background
Beauverin bassiana (Beauverin bassiana) is a broad-spectrum entomopathogenic fungus, can be parasitized on more than 700 harmful insects, has strong pathogenicity, strong adaptability and long lasting period, can control lepidoptera pests and coleoptera pests on various crops, such as cabbage caterpillar, plutella xylostella, cotton bollworm, corn borer, scarab, grub and mole cricket, and has special effect on pine moth. However, the insecticidal effective substance of the beauveria bassiana is live spores, so that the requirement on the application environment is higher, and the beauveria bassiana is dead naturally and ineffective at higher temperature, so that the effect of the beauveria bassiana is difficult to guarantee when the beauveria bassiana is singly used.
Paecilomyces fumosoroseus (Paecilomyces fumosa-roseus) is a pathogenic fungus with a wide host range, and hosts of the paecilomyces fumosoroseus-roseus comprise different insect states of various insects such as lepidoptera, diptera, coleoptera, hymenoptera, hemiptera and the like. After infecting host, the pathogenic fungus has strong pathogenicity and longer effective period, and has stronger infectivity to mantis and various pine caterpillars and stronger aphid killing effect. Similarly, the insecticidal effective substance of the paecilomyces fumosoroseus is live spores, so that the paecilomyces fumosoroseus has higher requirement on the application environment, and the paecilomyces fumosoroseus can die naturally and lose efficacy at higher temperature, and the effect of the paecilomyces fumosoroseus is difficult to guarantee when the paecilomyces fumosoroseus is singly used.
The single use of beauveria bassiana or paecilomyces fumosoroseus for killing insects has the defect of serious failure once the environment is changed due to high requirements on the application environment. Therefore, in order to overcome the defects of a single type of pesticide, the agricultural pesticide composition with broad spectrum and high efficiency is urgently needed to be provided.
Disclosure of Invention
In view of the above, the present invention aims to provide an agricultural pesticide composition.
The invention provides an agricultural insecticidal composition, which comprises the following components in parts by weight:
30-90 parts of pharmaceutical active components, wherein the pharmaceutical active components comprise paecilomyces fumosoroseus and beauveria bassiana in a weight ratio of (0.13-4.4) to (0.25-7.5);
10-70 parts of a drug carrier;
0.1 to 20 parts of an auxiliary agent.
Further, the paecilomyces fumosoroseus is selected from a strain with the biological preservation number of CGMCC No. 7993.
Further, the beauveria bassiana is selected from a strain with the biological preservation number of CGMCC NO.7997 and/or a strain with the biological preservation number of CGMCC NO. 7998.
Further, the preparation method of the paecilomyces fumosoroseus comprises the following steps:
1) activating the paecilomyces fumosoroseus strain;
2) inoculating the activated paecilomyces fumosoroseus into a paecilomyces fumosoroseus liquid culture medium for liquid fermentation, and culturing for 30-50 h to stop fermentation to obtain paecilomyces fumosoroseus fermentation liquor;
3) inoculating the paecilomyces fumosoroseus fermentation liquor obtained in the step 2) into a paecilomyces fumosoroseus solid culture medium, paving a tray, and carrying out shallow tray fermentation to obtain the paecilomyces fumosoroseus.
Further, the inoculation amount of the activated paecilomyces fumosoroseus inoculated in the liquid culture medium is 5-15%; the inoculation amount of the paecilomyces fumosoroseus fermentation liquor inoculated on the solid culture medium is 5-15%.
Further, the liquid culture medium of the paecilomyces fumosoroseus comprises the following components: 27-39 g/L of potato powder, 15-25 g/L of white granulated sugar, 0.5-1.5 g/L of peptone, 0.05-0.15 g/L of sodium nitrate, 0.20-0.30 g/L of potassium chloride, 0.01-0.10 g/L of magnesium sulfate, 0.01-0.10 g/L of manganese sulfate, 0.1-1.0 g/L of dipotassium hydrogen phosphate and 0.01-0.10 g/L of ferric sulfate.
Further, the solid culture medium of the paecilomyces fumosoroseus comprises the following components in parts by weight: 60-100 parts of wheat bran, 5-15 parts of corn flour and 5-15 parts of rice husk.
Further, the preparation method of the beauveria bassiana comprises the following steps:
1) activating the beauveria bassiana strain;
2) inoculating the activated beauveria bassiana into a beauveria bassiana liquid culture medium for liquid fermentation, and culturing for 30-50 h to stop fermentation to obtain a beauveria bassiana fermentation liquid;
3) inoculating the beauveria bassiana fermentation liquor obtained in the step 2) into a beauveria bassiana solid culture medium, spreading a tray, and performing shallow tray fermentation to obtain the beauveria bassiana.
Further, the inoculation amount of the activated beauveria bassiana inoculated in the liquid culture medium is 20-30%; the inoculation amount of the beauveria bassiana fermentation liquor inoculated in the solid culture medium is 20-30%.
Further, the drying step is also included after the solid fermentation of the beauveria bassiana or the paecilomyces fumosoroseus, the drying temperature is 30-50 ℃, and the drying time is 20-45 h.
Further, the liquid culture medium of beauveria bassiana comprises the following components: 30-50 g/L of soybean meal, 20-40 g/L of white granulated sugar, 0.01-0.05 g/L of flour, 0.01-0.05 g/L of potato powder and 0.0002g/L of dipotassium hydrogen phosphate.
Further, the solid culture medium of the beauveria bassiana comprises the following components in parts by weight: 50-90 parts of wheat bran, 10-30 parts of corn flour and 5-15 parts of rice husk.
Further, the drug carrier comprises one or more of calcium carbonate, diatomite, talcum powder, white carbon black, bentonite, sodium sulfate, argil and kaolin.
Further, the auxiliary agent comprises one or more of alkylbenzene sulfonate formaldehyde condensate, fatty alcohol-polyvinyl ether, alkyl naphthalene sulfonate and sodium lignosulphonate.
Furthermore, the agricultural insecticidal composition is applied to the prevention and treatment of crop pests.
The invention provides an agricultural insecticidal composition, which comprises the following components in parts by weight: 30-90 parts of pharmaceutical active components, wherein the pharmaceutical active components comprise paecilomyces fumosoroseus and beauveria bassiana in a weight ratio of (0.13-4.4) to (0.25-7.5); 10-70 parts of a drug carrier; 0.1 to 20 parts of an auxiliary agent. In the invention, the paecilomyces fumosoroseus and the beauveria bassiana are compounded, so that the obtained agricultural insecticidal composition has high efficiency, low toxicity, stable insecticidal effect, safety to people, livestock and crops, capability of protecting the crops, no harm to natural enemies, high safety and good environmental protection performance. Experimental results show that the agricultural insecticidal composition compounded by the paecilomyces fumosoroseus and the beauveria bassiana has the highest prevention effect of more than 85 percent and the insecticidal effect far higher than that of the agricultural insecticidal composition compounded by singly spraying the beauveria bassiana or the paecilomyces fumosoroseus.
Biological preservation Instructions
Paecilomyces fumosoroseus (Paecilomyces fumosoroseus) is preserved in China general microbiological culture Collection center at 13.08.2013.s.Committee, with the address of No. 3 Siro No. 1 of Beijing Kogyo-Yang district, and the biological preservation number of CGMCC No. 7993.
Beauveria bassiana (Beauveria bassiana) is preserved in the common microorganism center of China general microbiological culture Collection management Committee on year 2013, month 08 and day 13, and the address is No. 3 of West Lu No. 1 of Beijing city Kogyo-Yang district, and the microorganism research institute of Chinese academy of sciences, and the biological preservation number is CGMCC NO.7997 or CGMCC NO. 7998.
Detailed Description
The invention provides an agricultural insecticidal composition, which comprises the following components in parts by weight:
30-90 parts of pharmaceutical active components, wherein the pharmaceutical active components comprise paecilomyces fumosoroseus and beauveria bassiana in a weight ratio of (0.13-4.4) to (0.25-7.5); 10-70 parts of a drug carrier; 0.1 to 20 parts of an auxiliary agent.
The agricultural insecticidal composition provided by the invention comprises 30-90 parts of pharmaceutical active ingredients, preferably 50-70 parts, and more preferably 60 parts. In the invention, the pharmaceutical active components comprise paecilomyces fumosoroseus and beauveria bassiana in a weight ratio of (0.13-4.4): (0.25-7.5), and in the pharmaceutical active components, the weight ratio of the paecilomyces fumosoroseus to the beauveria bassiana is preferably (0.25-3.2): (1.5-6.0), more preferably (1.2-2.2): (2.5-4.5), most preferably 1.7: 3.0.
in the present invention, the paecilomyces fumosoroseus is selected from the strains with the biological preservation number of CGMCC No.7993, and in the embodiment, the paecilomyces fumosoroseus can be specifically a solid powdery paecilomyces fumosoroseus obtained by solid fermentation, drying and crushing.
In the invention, the preparation method of the paecilomyces fumosoroseus comprises the following steps:
1) activating the paecilomyces fumosoroseus strain;
2) inoculating the activated paecilomyces fumosoroseus into a paecilomyces fumosoroseus liquid culture medium for liquid fermentation, and culturing for 30-50 h to stop fermentation to obtain paecilomyces fumosoroseus fermentation liquor;
3) inoculating the paecilomyces fumosoroseus fermentation liquor obtained in the step 2) into a paecilomyces fumosoroseus solid culture medium, paving a tray, and carrying out shallow tray fermentation to obtain the paecilomyces fumosoroseus.
The activation of the paecilomyces fumosoroseus strain is to inoculate the preserved beauveria bassiana strain into an eggplant-shaped bottle filled with a nutrient agar culture medium, and to culture the strain at 25-28 ℃ for 8-12 h for activation culture.
In the invention, the inoculation amount of the activated paecilomyces fumosoroseus inoculated in the liquid culture medium is preferably 5-15%, more preferably 7-12%, and most preferably 10%; the inoculation amount of the paecilomyces fumosoroseus fermentation liquor inoculated on the solid culture medium is preferably 5-15%, more preferably 7-12%, and most preferably 10%.
In the present invention, the paecilomyces fumosoroseus liquid culture medium preferably comprises the following components: 27-39 g/L of potato powder, 15-25 g/L of white granulated sugar, 0.5-1.5 g/L of peptone, 0.05-0.15 g/L of sodium nitrate, 0.20-0.30 g/L of potassium chloride, 0.01-0.10 g/L of magnesium sulfate, 0.01-0.10 g/L of manganese sulfate, 0.1-1.0 g/L of dipotassium hydrogen phosphate and 0.01-0.10 g/L of ferric sulfate; more preferably 30-35 g/L of potato powder, 17-23 g/L of white granulated sugar, 0.8-1.2 g/L of peptone, 0.08-0.12 g/L of sodium nitrate, 0.23-0.27 g/L of potassium chloride, 0.03-0.07 g/L of magnesium sulfate, 0.03-0.07 g/L of manganese sulfate, 0.3-0.7 g/L of dipotassium hydrogen phosphate and 0.03-0.07 g/L of ferric sulfate; most preferably 32g/L of potato powder, 20g/L of white granulated sugar, 1.0g/L of peptone, 0.10g/L of sodium nitrate, 0.25g/L of potassium chloride, 0.05g/L of magnesium sulfate, 0.05g/L of manganese sulfate, 0.5g/L of dipotassium hydrogen phosphate and 0.05g/L of ferric sulfate.
In the invention, the liquid culture medium inoculated by the liquid fermentation of the paecilomyces fumosoroseus is preferably used after being sterilized for 30-45 min at 120-130 ℃. The present invention is not particularly limited to the apparatus for sterilizing the culture medium, and may be any apparatus known to those skilled in the art, such as an autoclave. The fermentation equipment is not particularly limited in the present invention, and fermentation equipment known in the art, such as a fermentation tank, may be used.
In the invention, the temperature of the paecilomyces fumosoroseus liquid fermentation is preferably 25-30 ℃, more preferably 26-29 ℃, and most preferably 28 ℃; the pH value of the Paecilomyces fumosoroseus liquid fermentation is preferably 6.6-7.2, more preferably 6.8-7.0, and most preferably 6.9; the pressure in the tank is preferably 0.05-0.1 MPa, and more preferably 0.07-0.08 MpPa; the ventilation volume of the liquid fermentation is preferably 0.8-1.2V/V.min, and more preferably 1V/V.min; the liquid fermentation time of the Paecilomyces fumosoroseus is preferably 30-50 h, more preferably 35-45 h, and more preferably 40 h.
After the liquid fermentation of the Paecilomyces fumosoroseus is finished, the obtained Paecilomyces fumosoroseus fermentation liquid is inoculated in a solid culture medium for shallow fermentation, and the solid fermentation Paecilomyces fumosoroseus is obtained. In the invention, the paecilomyces fumosoroseus solid culture medium preferably comprises the following components in parts by weight: 60-100 parts of wheat bran, 5-15 parts of corn flour and 5-15 parts of rice husk; more preferably 70-90 parts of wheat bran, 7-12 parts of corn flour and 7-12 parts of chaff; most preferably 80 parts of wheat bran, 10 parts of corn flour and 10 parts of rice husk. According to the invention, preferably, distilled water is added into the paecilomyces fumosoroseus solid culture medium to ensure that the water content of the solid culture medium is preferably 45-55%, more preferably 47-52%, and most preferably 50%, and then the solid culture medium is spread on a tray and subjected to tray fermentation. The distilled water to be added in the present invention is not particularly limited, and may be distilled water conventionally used by those skilled in the art.
In the invention, the temperature of the paecilomyces fumosoroseus solid fermentation is preferably 24-30 ℃, more preferably 26-29 ℃, and most preferably 28 ℃; the humidity of the paecilomyces fumosoroseus solid fermentation is preferably 40-95%, more preferably 55-75%, and most preferably 65%; the solid fermentation time of the paecilomyces fumosoroseus is preferably 48-96 h, more preferably 60-84 h, and most preferably 72 h.
After the solid fermentation of the paecilomyces fumosoroseus is finished, the obtained solid fermentation culture medium is preferably dried, crushed and sieved to obtain solid powdery paecilomyces fumosoroseus. In the invention, the drying temperature is preferably 30-50 ℃, the drying time is preferably 20-45 h, more preferably 35-45 ℃, 30-40 h, most preferably 40 ℃ and 35 h; according to the invention, the solid fermentation medium is dried until the moisture content is preferably 25-35%, more preferably 27-32%, and most preferably 30%. The method for pulverizing the solid fermentation medium is not particularly limited in the present invention, and a pulverization method known to those skilled in the art may be used. In the invention, the solid powdery paecilomyces fumosoroseus is sieved by using 80-100 meshes preferably, and 85-95 meshes more preferably. Most preferably 90 mesh.
In the invention, the beauveria bassiana is selected from the strains with the biological preservation number of CGMCC NO.7997 and/or the biological preservation number of CGMCC NO.7998, and in the embodiment, the solid powdery beauveria bassiana obtained by solid fermentation, drying and crushing can be specifically adopted.
In the invention, the preparation method of the beauveria bassiana comprises the following steps:
1) activating the beauveria bassiana strain;
2) inoculating the activated beauveria bassiana into a beauveria bassiana liquid culture medium for liquid fermentation, and culturing for 30-50 h to stop fermentation to obtain a beauveria bassiana fermentation liquid;
3) inoculating the beauveria bassiana fermentation liquor obtained in the step 2) into a beauveria bassiana solid culture medium, spreading a tray, and performing shallow tray fermentation to obtain the beauveria bassiana.
The activation of the beauveria bassiana strain is to inoculate the preserved beauveria bassiana strain into an eggplant-shaped bottle filled with a nutrient agar culture medium, and culture the strain at 25-28 ℃ for 8-12 h for activation culture.
In the invention, the inoculation amount of the activated beauveria bassiana inoculated in the liquid culture medium is preferably 20-30%, more preferably 23-27%, and most preferably 25%; the inoculation amount of the beauveria bassiana fermentation liquor inoculated on the solid culture medium is preferably 20-30%, more preferably 23-27%, and most preferably 25%.
In the present invention, the beauveria bassiana liquid medium preferably includes the following components: 30-50 g/L of soybean meal, 20-40 g/L of white granulated sugar, 0.01-0.05 g/L of flour, 0.01-0.05 g/L of potato powder and 0.0002g/L of dipotassium hydrogen phosphate; more preferably: 35-45 g/L of soybean meal, 25-35 g/L of white granulated sugar, 0.02-0.04 g/L of flour, 0.02-0.04 g/L of potato powder and 0.0002g/L of dipotassium hydrogen phosphate; most preferably: 40g/L of soybean meal, 30g/L of white granulated sugar, 0.03g/L of flour, 0.03g/L of potato powder and 0.0002g/L of dipotassium hydrogen phosphate.
In the invention, the liquid culture inoculated by the liquid fermentation of the beauveria bassiana is preferably used after being sterilized for 30-45 min at 120-130 ℃. The apparatus for sterilizing the culture medium in the present invention is not particularly limited, and any sterilization apparatus known to those skilled in the art, such as an autoclave, may be used. The fermentation equipment is not particularly limited in the present invention, and fermentation equipment known to those skilled in the art, such as a fermentation tank, may be used.
In the invention, the temperature of the paecilomyces fumosoroseus liquid fermentation is preferably 25-30 ℃, more preferably 26-29 ℃, and most preferably 28 ℃; the pH value of the Paecilomyces fumosoroseus liquid fermentation is preferably 6.6-7.2, more preferably 6.8-7.0, and most preferably 6.9; the pressure in the tank is preferably 0.05-0.1 MPa, and more preferably 0.07-0.08 MpPa; the ventilation volume of the liquid fermentation is preferably 0.8-1.2V/V.min, and more preferably 1V/V.min; the time for liquid fermentation is preferably 30-50 h, more preferably 35-45 h, and most preferably 40 h.
After the liquid fermentation of the beauveria bassiana is finished, the obtained beauveria bassiana fermentation liquid is inoculated in a solid culture medium for shallow tray fermentation, and the solid fermentation beauveria bassiana is obtained. In the present invention, the beauveria bassiana solid medium preferably comprises the following components in parts by weight: 50-90 parts of wheat bran, 10-30 parts of corn flour and 5-15 parts of rice husk; more preferably 60-80 parts of wheat bran, 15-25 parts of corn flour and 7-13 parts of chaff; most preferably 70 parts of wheat bran, 20 parts of corn flour and 10 parts of rice husk. According to the invention, distilled water is added into the beauveria bassiana solid culture medium to ensure that the water content of the solid culture medium is preferably 45-55%, more preferably 47-52%, and most preferably 50%, and then the solid culture medium is spread on a tray and is subjected to shallow tray fermentation. The distilled water to be added in the present invention is not particularly limited, and may be distilled water conventionally used by those skilled in the art.
In the invention, the temperature of the beauveria bassiana solid fermentation is preferably 24-30 ℃, more preferably 26-29 ℃, and most preferably 28 ℃; the humidity of the beauveria bassiana solid fermentation is preferably 40-95%, more preferably 55-75%, and most preferably 65%; the time for the beauveria bassiana solid fermentation is preferably 48-96 h, more preferably 60-84 h, and most preferably 72 h.
After the beauveria bassiana solid fermentation is completed, the obtained beauveria bassiana solid fermentation culture medium is preferably dried, crushed and sieved to obtain solid powdery beauveria bassiana. In the invention, the drying temperature is preferably 30-50 ℃, the drying time is preferably 20-45 h, more preferably 35-45 ℃, 30-40 h, most preferably 40 ℃ and 35 h; according to the invention, the solid fermentation medium is dried until the moisture content is preferably 25-35%, more preferably 27-32%, and most preferably 30%. The crushing method of the beauveria bassiana solid fermentation medium is not particularly limited, and the crushing method known by the person skilled in the art can be adopted. In the invention, the solid powdery beauveria bassiana is preferably sieved by 80-100 meshes, more preferably 85-95 meshes, and most preferably 90 meshes.
The agricultural insecticidal composition provided by the invention preferably comprises 10-70 parts of a drug carrier, more preferably 30-50 parts, and most preferably 40 parts. In the present invention, the drug carrier is not particularly limited, and a commercially available product may be used. Specifically, one or more of light calcium carbonate, diatomite, talcum powder, white carbon black, bentonite, sodium sulfate, argil and kaolin can be preferably selected. The pharmaceutical carrier may be any of the above substances, and may be mixed in any proportion. The drug carrier provided by the invention has good drug stability and can also improve the drug effect of the drug.
The agricultural insecticidal composition provided by the invention preferably comprises 0.1-20 parts of an auxiliary agent, more preferably 5-15 parts, and most preferably 10 parts. In the present invention, the auxiliary agent is not particularly limited, and commercially available products may be used. Specifically, the formaldehyde condensate can be one or more of alkylbenzene sulfonate, fatty alcohol-polyvinyl ether, alkyl naphthalene sulfonate and sodium lignosulphonate. The adjuvants may be any of the above substances, and may be mixed in any proportion. In the present invention, the adjuvant stabilizes the drug properties and improves the drug efficacy.
The preparation method of the agricultural insecticidal composition is not particularly limited, and the preparation method of the composition known to those skilled in the art can be adopted. The pharmaceutical composition can be prepared by mixing the pharmaceutical active component, the pharmaceutical carrier and the auxiliary agent.
The agricultural insecticidal composition can be applied to the aspect of preventing and controlling the insect pests of crops, and provides the application of the agricultural insecticidal composition in the aspect of preventing and controlling the insect pests of the crops. In particular, the applicable crop species include grain crops, commercial crops, vegetable crops, fruit crops, forage crops, and the like. The agricultural insecticidal composition can control various pests and mites, including cabbage caterpillar, diamondback moth, cotton bollworm, corn borer, scarab beetle, red spider, pine moth, mantis ostriniae, aphid, grub and mole cricket which are underground pests.
The method for applying the agricultural insecticidal composition is not particularly limited, and the method for applying the agricultural insecticidal composition is known to those skilled in the art. The method specifically comprises the following steps: the agricultural insecticidal composition is diluted by water to obtain 1000-5000 times of diluent, and then is sprayed to leaf surfaces.
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Preparing solid powdery Paecilomyces fumosoroseus, inoculating the preserved Paecilomyces fumosoroseus strain into eggplant-shaped bottle filled with nutrient agar culture medium, and culturing at 28 deg.C for 12 hr for activation culture; inoculating the activated paecilomyces fumosoroseus into a liquid culture medium sterilized at 121 ℃ for 35min by 10 percent of inoculation amount, and performing liquid fermentation in a fermentation tank under the specific fermentation conditions: the temperature is 28 ℃; the pH value is 6.9; the pressure in the tank is 0.08MPa, and the fermentation ventilation is 1V/V.min; stopping fermentation after fermenting for 40h to obtain Paecilomyces fumosoroseus fermentation liquor; inoculating the obtained paecilomyces fumosoroseus fermentation liquor into a solid culture medium in an inoculation amount of 10%, adding distilled water into the solid culture medium to ensure that the water content of the solid culture medium is 50%, then spreading a tray, and carrying out tray fermentation under the fermentation conditions that: the fermentation temperature is 28 ℃, the fermentation humidity is 60%, and the fermentation time is 72 h; and after the solid fermentation is finished, drying the obtained solid fermented paecilomyces fumosoroseus for 35h at 40 ℃ until the water content of the solid fermented paecilomyces fumosoroseus is 30%, crushing and sieving by a 100-mesh sieve to obtain solid powdery paecilomyces fumosoroseus.
Example 2
Preparing solid powdery beauveria bassiana by inoculating the preserved beauveria bassiana strain into an eggplant-shaped bottle filled with a nutrient agar culture medium, and culturing at 26 ℃ for 9h for activation culture; inoculating the activated and cultured beauveria bassiana in a liquid culture medium sterilized at 121 ℃ for 35min by 25 percent of inoculation amount, and performing liquid fermentation in a fermentation tank, wherein the specific fermentation conditions are as follows: the temperature is 28 ℃; the pH value is 6.9; the pressure in the tank is 0.08MPa, and the fermentation ventilation is 1V/V.min; stopping fermentation after fermenting for 40h to obtain a beauveria bassiana fermentation broth; inoculating the obtained beauveria bassiana fermentation liquor into a solid culture medium in an inoculation amount of 25%, adding distilled water into the solid culture medium to ensure that the water content of the solid culture medium is 50%, then paving a tray, and carrying out tray fermentation under the fermentation conditions: the fermentation temperature is 26 ℃, the fermentation humidity is 65%, and the fermentation time is 72 h; and after the solid fermentation is finished, drying the obtained solid fermented beauveria bassiana for 35 hours at the temperature of 40 ℃, drying until the water content of the solid fermented beauveria bassiana is 30%, and crushing and sieving by a 90-mesh sieve to obtain solid powdery beauveria bassiana.
Example 3
Preparation of agricultural insecticidal composition: by weight, 25.3 parts of solid paecilomyces fumosoroseus prepared in example 1, 44.7 parts of beauveria bassiana prepared in example 2, 20 parts of calcium carbonate and 10 parts of fatty alcohol-polyvinyl ether are mixed to obtain the agricultural insecticidal composition.
Example 4
Preparation of agricultural insecticidal composition: 28.9 parts of solid paecilomyces fumosoroseus prepared in example 1, 51.1 parts of beauveria bassiana prepared in example 2, 7.5 parts of diatomite, 7.5 parts of white carbon black and 5 parts of sodium lignosulphonate are mixed in parts by weight to obtain the agricultural insecticidal composition.
Example 5
Preparation of agricultural insecticidal composition: 27.1 parts of solid paecilomyces fumosoroseus prepared in example 1, 47.9 parts of beauveria bassiana prepared in example 2, 7 parts of talcum powder, 5 parts of sodium sulfate, 10 parts of kaolin and 3 parts of alkylbenzene sulfonate formaldehyde condensate are mixed in parts by weight to obtain the agricultural insecticidal composition.
Example 6
Preparation of agricultural insecticidal composition: by weight, 21.7 parts of solid paecilomyces fumosoroseus prepared in example 1, 38.3 parts of beauveria bassiana prepared in example 2, 8 parts of talcum powder, 10 parts of white carbon black, 7 parts of bentonite, 7 parts of argil, 2 parts of fatty alcohol-polyvinyl ether, 3 parts of alkyl naphthalene sulfonate and 3 parts of sodium lignosulphonate are mixed to obtain the agricultural insecticidal composition.
Example 7
And (3) pharmacodynamic test: the test is carried out in Jiangxi province, the prevention and control variety is cotton, the management is good, and the occurrence of cotton bollworms is medium and heavy. In the test, the experimental field is divided into four areas, the area 1 is a control group, and only clear water is sprayed; zone 2 was sprayed with 2000-fold dilution of the pesticidal composition prepared in example 3; spraying a composition which does not contain paecilomyces fumosoroseus and only contains beauveria bassiana, a carrier and an auxiliary agent in the area 3, wherein the concentration of the active pharmaceutical ingredients is the same as that in the area 2; spraying a composition which does not contain beauveria bassiana and only contains paecilomyces fumosoroseus, a carrier and an auxiliary agent in the area 4, wherein the concentration of the active pharmaceutical ingredients is the same as that in the areas 2 and 3; the dosages of the four areas are the same, the number of live insects is respectively investigated for 1 day, 3 days, 5 days and 7 days after the application of the pesticide, and the reduction rate of the population of the insects is calculated. The specific results are shown in table 1, and it can be seen from the data in table 1 that the population reduction rate in the area 2 is the highest, which indicates that the pesticidal composition prepared by the present invention has the best pesticidal effect, and the pesticide effect is higher than that of the separately sprayed beauveria bassiana or paecilomyces fumosoroseus with the same concentration.
TABLE 1 insecticidal (Heliothis armigera) Effect of the agricultural insecticidal compositions
From the test data in table 1, it can be seen that the population reduction rate of the agricultural insecticidal composition prepared by the invention in 7 days can reach as high as 85.7%, and the insecticidal effect is far higher than that of paecilomyces fumosoroseus or beauveria bassiana which is singly sprayed. Example 8
And (3) pharmacodynamic test: the test is carried out in Jiangxi province, the prevention and control variety is plutella xylostella, the management is good, and the occurrence of the plutella xylostella is medium and serious. In the test, the experimental field is divided into four areas, the area 1 is a control group, and only clear water is sprayed; spraying 3000 times of diluent of the pesticide insecticidal composition prepared in the example 4 to the area 2; spraying a composition which does not contain paecilomyces fumosoroseus and only contains beauveria bassiana, a carrier and an auxiliary agent in the area 3, wherein the concentration of the active pharmaceutical ingredients is the same as that in the area 2; spraying a composition which does not contain beauveria bassiana and only contains paecilomyces fumosoroseus, a carrier and an auxiliary agent in the area 4, wherein the concentration of the active pharmaceutical ingredients is the same as that in the areas 2 and 3; the dosages of the four areas are the same, the number of live insects is respectively investigated for 1 day, 3 days, 5 days and 7 days after the application of the pesticide, and the reduction rate of the population of the insects is calculated. The specific results are shown in table 2, and the data in table 2 show that the insect population regression rate in the area 2 is the highest, which indicates that the pesticide insecticidal composition prepared by the invention has the best insecticidal effect, and the pesticide effect is higher than that of beauveria bassiana or paecilomyces fumosoroseus which is sprayed alone and has the same concentration.
TABLE 2 insecticidal (diamondback moth) Effect of the agricultural insecticidal compositions
From the test data in table 2, it can be seen that the population reduction rate of the agricultural insecticidal composition prepared by the invention in 7 days can reach as high as 85.1%, and the insecticidal effect is far higher than that of paecilomyces fumosoroseus or beauveria bassiana which is singly sprayed.
As can be seen from the above examples, the agricultural insecticidal composition prepared by the invention has a control rate of insect pests as high as more than 85%, and the insecticidal effect is far higher than that of Paecilomyces fumosoroseus or Beauveria bassiana which is singly used.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (4)
1. An agricultural insecticidal composition comprises the following components in parts by weight:
25.3 parts of solid powdery paecilomyces fumosoroseus, 44.7 parts of solid powdery beauveria bassiana, 20 parts of calcium carbonate and 10 parts of fatty alcohol-polyvinyl ether;
the preparation method of the solid powdery paecilomyces fumosoroseus comprises inoculating the preserved paecilomyces fumosoroseus strain into an eggplant-shaped bottle filled with a nutrient agar culture medium, and culturing at 28 ℃ for 12h for activation culture; inoculating the activated paecilomyces fumosoroseus into a liquid culture medium sterilized at 121 ℃ for 35min by 10 percent of inoculation amount, and performing liquid fermentation in a fermentation tank under the specific fermentation conditions: the temperature is 28 ℃; the pH value is 6.9; the pressure in the tank is 0.08MPa, and the fermentation ventilation is 1V/V.min; stopping fermentation after fermenting for 40h to obtain Paecilomyces fumosoroseus fermentation liquor; inoculating the obtained paecilomyces fumosoroseus fermentation liquor into a solid culture medium in an inoculation amount of 10%, adding distilled water into the solid culture medium to ensure that the water content of the solid culture medium is 50%, then spreading a tray, and carrying out tray fermentation under the fermentation conditions that: the fermentation temperature is 28 ℃, the fermentation humidity is 60%, and the fermentation time is 72 h; after the solid fermentation is finished, drying the obtained solid fermented paecilomyces fumosoroseus for 35h at 40 ℃ until the water content of the solid fermented paecilomyces fumosoroseus is 30%, crushing and sieving by a 100-mesh sieve to obtain solid powdery paecilomyces fumosoroseus;
the preparation method of the solid powdery beauveria bassiana comprises inoculating the preserved beauveria bassiana strain into an eggplant-shaped bottle filled with a nutrient agar culture medium, and culturing at 26 deg.C for 9h for activation culture; inoculating the activated and cultured beauveria bassiana in a liquid culture medium sterilized at 121 ℃ for 35min by 25 percent of inoculation amount, and performing liquid fermentation in a fermentation tank, wherein the specific fermentation conditions are as follows: the temperature is 28 ℃; the pH value is 6.9; the pressure in the tank is 0.08MPa, and the fermentation ventilation is 1V/V.min; stopping fermentation after fermenting for 40h to obtain a beauveria bassiana fermentation broth; inoculating the obtained beauveria bassiana fermentation liquor into a solid culture medium in an inoculation amount of 25%, adding distilled water into the solid culture medium to ensure that the water content of the solid culture medium is 50%, then paving a tray, and carrying out tray fermentation under the fermentation conditions: the fermentation temperature is 26 ℃, the fermentation humidity is 65%, and the fermentation time is 72 h; after the solid fermentation is finished, drying the obtained solid fermented beauveria bassiana for 35 hours at the temperature of 40 ℃, drying until the water content of the solid fermented beauveria bassiana is 30%, crushing and sieving by a 90-mesh sieve to obtain solid powdery beauveria bassiana;
the paecilomyces fumosoroseus is selected from a strain with the biological preservation number of CGMCC No. 7993;
the beauveria bassiana is selected from a strain with a biological preservation number of CGMCC NO.7997 and/or a strain with a biological preservation number of CGMCC NO. 7998.
2. The agricultural insecticidal composition according to claim 1, wherein the liquid medium of paecilomyces fumosoroseus comprises the following components: 27-39 g/L of potato powder, 15-25 g/L of white granulated sugar, 0.5-1.5 g/L of peptone, 0.05-0.15 g/L of sodium nitrate, 0.20-0.30 g/L of potassium chloride, 0.01-0.10 g/L of magnesium sulfate, 0.01-0.10 g/L of manganese sulfate, 0.1-1.0 g/L of dipotassium hydrogen phosphate and 0.01-0.10 g/L of ferric sulfate;
the solid culture medium of the paecilomyces fumosoroseus comprises the following components in parts by weight: 60-100 parts of wheat bran, 5-15 parts of corn flour and 5-15 parts of rice husk.
3. An agricultural insecticidal composition according to claim 1, wherein the liquid medium of beauveria bassiana comprises the following components: 30-50 g/L of soybean meal, 20-40 g/L of white granulated sugar, 0.01-0.05 g/L of flour, 0.01-0.05 g/L of potato powder and 0.0002g/L of dipotassium hydrogen phosphate;
the solid culture medium of the beauveria bassiana comprises the following components in parts by weight: 50-90 parts of wheat bran, 10-30 parts of corn flour and 5-15 parts of rice husk.
4. Use of the agricultural pesticidal composition of any one of claims 1 to 3 for controlling pests on crops.
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| CN104073439A (en) * | 2013-12-06 | 2014-10-01 | 江西天人生态股份有限公司 | Beauveria bassiana(Bals.-Criv.)Vuill and application thereof |
| CN104073440A (en) * | 2013-12-06 | 2014-10-01 | 江西天人生态股份有限公司 | Paecilomyces fumosoroseus and application thereof |
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| CN102341493A (en) * | 2008-10-14 | 2012-02-01 | 格林泰德有限公司 | Entomopathogenic fungi and uses thereof |
| CN104073439A (en) * | 2013-12-06 | 2014-10-01 | 江西天人生态股份有限公司 | Beauveria bassiana(Bals.-Criv.)Vuill and application thereof |
| CN104073440A (en) * | 2013-12-06 | 2014-10-01 | 江西天人生态股份有限公司 | Paecilomyces fumosoroseus and application thereof |
| CN104087510A (en) * | 2013-12-06 | 2014-10-08 | 江西天人生态股份有限公司 | Beauveria bassiana and application thereof |
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