CN105738165B - A kind of full-automatic bioaerosol sampling and detection robot device - Google Patents

A kind of full-automatic bioaerosol sampling and detection robot device Download PDF

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Publication number
CN105738165B
CN105738165B CN201410757006.3A CN201410757006A CN105738165B CN 105738165 B CN105738165 B CN 105738165B CN 201410757006 A CN201410757006 A CN 201410757006A CN 105738165 B CN105738165 B CN 105738165B
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sampling
liquid
bioaerosol
automatic
full
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CN105738165A (en
Inventor
曹务春
刘毅
方立群
刘航
李新楼
刘强
张晓清
宁海波
刘爱明
刘昆
姚宏武
孙烨
陈婉君
孙若曦
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Beijing Huifenglong Economic And Technology Development Co ltd
Institute of Microbiology and Epidemiology of AMMS
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Beijing Huifenglong Economic And Technology Development Co ltd
Institute of Microbiology and Epidemiology of AMMS
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Abstract

The present invention discloses a kind of full-automatic bioaerosol sampling and detection robot device, including traveling unit, sampling and verification unit and video acquisition unit, wherein sampling and verification unit include particle cutter, wind turbine, sampling bottle, aspiration pump, syringe pump, liquid storage bottle, peristaltic pump, mechanism for filling liquid, photoelectric detection system, transmission mechanism and inspection card device, it is realized under the collective effect of two-way major-minor gas circuit and concentration acquisition is carried out to the bioaerosol particle in air, sample solution is conveyed into mechanism for filling liquid automatically by liquid delivery path, mechanism for filling liquid carries out the distribution that sample liquid is realized in cooperation with transmission mechanism, photoelectric detection mechanism realizes biological species identification with transmission mechanism cooperation, whole device identifies the automation with height from sample collection to biological species.

Description

A kind of full-automatic bioaerosol sampling and detection robot device
Technical field
The present invention relates to bio-safety fields, in particular to a kind of full-automatic bioaerosol sampling and detection machine Device people's device.
Background technology
Since the 1970s, emerging infectious disease takes place frequently, public health event (such as " SARS ", HPAI (High Pathogenic AI) Deng) happen occasionally, while natural calamity takes place frequently, thus derivative people, animal epidemic situation produces the environment of the mankind and its existence Significant impact, in addition bio-terror threats are serious.China be faced with bio-terrorism, public health event, secondary biohazard, from A variety of bio-safeties such as the biohazard of right disaster derivation threaten.
The prevention key of bio-safety be it is early find, early disposition, therefore first time for occurring in event it should be understood that To the pollution condition at scene, site of the accident bad environments are unfavorable for personnel's entrance, while to staff's health and life Safety causes greatly to threaten.
Invention content
A kind of full-automatic bioaerosol sampling of present invention offer and detection robot device, it can be existing into biological event Field carries out concentration acquisition to bioaerosol particle and biological species are identified, to improve unexpected biological event field emergency disposal energy Power..
In order to achieve the above objectives, it samples the present invention provides a kind of full-automatic bioaerosol and detects robot device, Including traveling unit, sampling and verification unit and video acquisition unit, wherein:
The sampling is mounted on by damper on the traveling unit with verification unit, and the traveling unit is provided with rubber Glue crawler belt;
The sampling and verification unit include particle cutter, wind turbine, sampling bottle, aspiration pump, syringe pump, liquid storage bottle, compacted Dynamic pump, mechanism for filling liquid, photoelectric detection system, transmission mechanism and inspection card device;
The sampling includes main gas circuit and secondary gas circuit with verification unit, wherein the main airflow exit warp of the particle cutter The sampling bottle and the aspiration pump are connected to form main gas circuit, and the secondary streams outlet of the particle cutter is connected with the wind turbine Form secondary gas circuit;
The sampling includes also a flow path of the liquid with verification unit, wherein liquid passes through the note in the liquid storage bottle It penetrates pump to enter in the sampling bottle, liquid extraction in the sampling bottle, which is transported to the mechanism for filling liquid, by the peristaltic pump realizes The distribution of sample liquid;
The sampling bottle includes inlet, liquid outlet, air inlet and gas outlet, wherein the inlet and the syringe pump It is connected, the liquid outlet is connected with the peristaltic pump, stretches into the air inlet of the sampling bottle bottom end and the main airflow exit phase Even, the gas outlet is connected with the aspiration pump inlet end, and sample liquid first passes through the syringe pump when acquiring will be in the liquid storage bottle Buffer solution inject in the sampling bottle, negative pressure is then formed in sampling bottle liquid level upper end by the aspiration pump, at this time The inlet port being connected with the particle cutter main airflow exit is atmospheric pressure, is injected by the collected sample gas of pressure difference general who has surrendered Sample liquid is formed in buffer solution;
The mechanism for filling liquid is vacantly set to the transmission mechanism upper end with the photoelectric detection system, and the inspection clamps It sets and is fixedly installed on the transmission mechanism, the transmission mechanism drives the inspection card device side-to-side movement, the filling machine Sample liquid is injected separately into test point by structure realization, and photoelectric detection system, which is realized, is detected the sample liquid for injecting test point;
The transmission mechanism includes two sliding rails, a guide rail, a motor and two limit switches, and the detection clamps It sets on two sliding rails, which is arranged in guide rail, can horizontally slip along the guide rail, and institute is driven by the motor Detection card device is stated along guide rail side-to-side movement, when the limit switch that the shielding plate on the detection card device shelters from left side then stops It only moves downward, when the limit switch that the shielding plate on the detection card device shelters from right side then stops moving right.
Further, the sampling is used to carry out concentration acquisition and life to air bioaerosol particle with verification unit Species are not examined.
Further, the traveling unit is for realizing automatic motor function.
Further, the video acquisition unit for monitoring the traveling road conditions of the traveling unit in real time.
Further, the damper is damping spring.
Further, the flow-rate ratio ranging from 1 of the main gas circuit and the secondary gas circuit:20-7:200.
Further, the buffer solution injection rate is 5ml-25ml.
The invention has the advantages that:
(1) full-automatic bioaerosol sampling provided by the invention and detection robot device, are integrated with bioaerosol The function of concentration acquisition and biological species identification, can obtain the polluted information at biological event scene faster, quickly convenient for scene Emergency disposal;
(2) the degree of automation of the present invention is higher, from sample collection to output testing result, is operated without personnel;
(3) present invention can enter in a variety of adverse circumstances instead of staff and work.Improve the safety of personnel.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with Obtain other attached drawings according to these attached drawings.
Fig. 1 is the structure chart of the sampling of full-automatic bioaerosol and detection robot device of one embodiment of the invention;
Fig. 2 a, Fig. 2 b are sampling and the verification unit structure chart of one embodiment of the invention;
Fig. 3 is the particle cutter schematic diagram of one embodiment of the invention;
Fig. 4 is the sampling bottle structure principle chart of one embodiment of the invention;
Fig. 5 is the transmission mechanism structure principle chart of one embodiment of the invention.
Figure label explanation:1, traveling unit;2, sampling and verification unit;3, video acquisition unit;4, damper;5, grain Sub- cutter;6, wind turbine;7, sampling bottle;8, aspiration pump;9, syringe pump;10, liquid storage bottle;11, peristaltic pump;12, mechanism for filling liquid; 13, photoelectric detection system;14, transmission mechanism;15, card device is examined;5.1, main airflow exit;5.2, secondary streams export;7.1、 Sampling bottle inlet;7.2, sampling bottle air inlet;7.3, sampling bottle gas outlet;7.4, sampling bottle liquid outlet;14.1, sliding rail; 14.2, guide rail;14.3, motor;14.4, left limit switchs;14.5, right limit switchs.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of not making the creative labor Embodiment shall fall within the protection scope of the present invention.
Fig. 1 is the sampling of full-automatic bioaerosol and the detection robot schematic device of one embodiment of the invention;Such as Shown in figure, including traveling unit 1, sampling and verification unit 2 and video acquisition unit 3, wherein:
The bioaerosol sampling is designed with two-stage damping with detection robot device, and wherein traveling unit 1 uses rubber Glue crawler belt integrally carries out damping to the device, and the sampling is mounted on institute with verification unit 2 by damper (such as damping spring) 4 It states in traveling, two level damping is carried out to sampling and verification unit by damper, robot is reduced by the design of two level damping Device is in the traveling of different complex road conditions to sampling the influence with precise part in verification unit;
As shown in Fig. 2 a, b, it is described sampling with verification unit include particle cutter 5, wind turbine 6, sampling bottle 7, aspiration pump 8, Syringe pump 9, liquid storage bottle 10, peristaltic pump 11, mechanism for filling liquid 12, photoelectric detection system 13, transmission mechanism 14 and inspection card device 15;
The sampling includes main gas circuit and secondary gas circuit with verification unit, wherein the main airflow exit of the particle cutter 5 5.1 are connected to form main gas circuit through the sampling bottle 7 and the aspiration pump 8, the secondary streams outlet 5.2 of the particle cutter 5 with The wind turbine 6 is connected to form secondary gas circuit, preferred main gas circuit and secondary gas circuit flow-rate ratio ranging from 1:20-7:200;
The sampling also includes a flow path of the liquid with verification unit, wherein liquid is described in the liquid storage bottle 10 Syringe pump 9 enters in the sampling bottle 7, and liquid extraction in the sampling bottle 7 is transported to the fluid injection by the peristaltic pump 11 Mechanism 12 realizes the distribution of sample liquid;
The sampling bottle 7 includes inlet 7.1, liquid outlet 7.4, air inlet 7.2 and gas outlet 7.3, wherein the feed liquor Mouth 7.1 is connected with the syringe pump 8, and the liquid outlet 7.4 is connected with the peristaltic pump 11, stretches into 7 bottom end of the sampling bottle Air inlet 7.2 is connected with the main airflow exit 5.1, and the gas outlet 7.3 is connected with 8 inlet end of the aspiration pump, and sample liquid is adopted The syringe pump 8 is first passed through when collection to inject the buffer solution in the liquid storage bottle 10 in the sampling bottle 7, then passes through the pumping Air pump 87 liquid level upper end of the sampling bottle formed negative pressure, be connected at this time with 5 main airflow exit 5.1 of the particle cutter into Gas port end is atmospheric pressure, is injected in buffer solution by the collected sample gas of pressure difference general who has surrendered and forms sample liquid, and preferred Discharge rate is 5- 25ml;
The mechanism for filling liquid 12 is vacantly set to 14 upper end of the transmission mechanism, the inspection with the photoelectric detection system 13 It tests card device 15 to be fixedly installed on the transmission mechanism 14, the transmission mechanism 14 drives the inspection card device 15 or so to transport Dynamic, sample liquid is injected separately into test point by the realization of the mechanism for filling liquid 12, and photoelectric detection system 13 is realized to injection test point Sample liquid is detected;
As shown in figure 5, the transmission mechanism 14 includes two 14.2, one, the guide rail of sliding rail 14.1, one, 14.3 and of motor Two limit switches 14.4,14.5, the detection card device 15 are mounted on two sliding rails 14.1, which is arranged It in guide rail 14.2, can horizontally slip along the guide rail 14.2, drive the detection card device 15 along guide rail by the motor 14.3 Side-to-side movement, the limit switch 14.4 on the left of the shielding plate on the detection card device 15 shelters from then stop moving downward, When the limit switch 14.5 that the shielding plate on the detection card device 15 shelters from right side then stops moving right.
Further, it is described sampling with verification unit 2 be used for air bioaerosol particle carry out concentration acquisition and Category is examined.
Further, the traveling unit 1 is for realizing automatic motor function.
Further, the video acquisition unit 3 for supervising the traveling road conditions of the traveling unit 1 in real time It surveys.
The full-automatic bioaerosol sampling of the present invention and detection robot device, in the collective effect of two-way major-minor gas circuit Lower realization carries out concentration acquisition to the bioaerosol particle in air, is automatically conveyed into sample solution by liquid delivery path In mechanism for filling liquid, mechanism for filling liquid carries out the distribution that sample liquid is realized in cooperation with transmission mechanism, and photoelectric detection mechanism is matched with transmission mechanism It closes and realizes that biological species identification, whole device identify the automation with height from sample collection to biological species, it can be more rapidly Determination suspected pollution source, be applied to love scene will greatly improve bio-safety prevention and control capability, it is ensured that natural calamity, life The sampling of object safety and the bio-safety of production accident, public health emergency and social security events scene is examined.
One of ordinary skill in the art will appreciate that:Attached drawing is the schematic diagram of one embodiment, module in attached drawing or Flow is not necessarily implemented necessary to the present invention.
One of ordinary skill in the art will appreciate that:The module in device in embodiment can describe to divide according to embodiment It is distributed in the device of embodiment, respective change can also be carried out and be located in one or more devices different from the present embodiment.On The module for stating embodiment can be merged into a module, can also be further split into multiple submodule.
Finally it should be noted that:The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations;Although Present invention has been described in detail with reference to the aforementioned embodiments, it will be understood by those of ordinary skill in the art that:It still may be used To modify to the technical solution recorded in previous embodiment or equivalent replacement of some of the technical features;And These modifications or replacements, the spirit and model of technical solution of the embodiment of the present invention that it does not separate the essence of the corresponding technical solution It encloses.

Claims (7)

1. a kind of full-automatic bioaerosol sampling and detection robot device, which is characterized in that including traveling unit (1), adopt Sample and verification unit (2) and video acquisition unit (3), wherein:
The sampling is mounted on by damper (4) on the traveling unit (1) with verification unit (2), the traveling unit (1) It is provided with rubber belt track;
The sampling and verification unit include particle cutter (5), wind turbine (6), sampling bottle (7), aspiration pump (8), syringe pump (9), liquid storage bottle (10), peristaltic pump (11), mechanism for filling liquid (12), photoelectric detection system (13), transmission mechanism (14) and check card Device (15);
The sampling includes main gas circuit and secondary gas circuit with verification unit, wherein the main airflow exit of the particle cutter (5) (5.1) it is connected to form main gas circuit through the sampling bottle (7) and the aspiration pump (8), the secondary streams of the particle cutter (5) go out Mouth (5.2) is connected to form secondary gas circuit with the wind turbine (6);
The sampling includes also a flow path of the liquid with verification unit, wherein liquid passes through the note in the liquid storage bottle (10) It penetrates pump (9) to enter in the sampling bottle (7), liquid extraction in the sampling bottle (7) is transported to institute by the peristaltic pump (11) State the distribution that mechanism for filling liquid (12) realizes sample liquid;
The sampling bottle (7) includes inlet (7.1), liquid outlet (7.4), air inlet (7.2) and gas outlet (7.3), wherein institute It states inlet (7.1) with the syringe pump (9) to be connected, the liquid outlet (7.4) is connected with the peristaltic pump (11), stretches into described The air inlet (7.2) of sampling bottle (7) bottom end is connected with the main airflow exit (5.1), the gas outlet (7.3) and the pumping It pumps (8) inlet end to be connected, sample liquid first passes through the syringe pump (9) and the buffer solution in the liquid storage bottle (10) is injected institute when acquiring State in sampling bottle (7), then by the aspiration pump (8) the sampling bottle (7) liquid level upper end formed negative pressure, at this time with it is described The connected inlet port of particle cutter (5) main airflow exit (5.1) is atmospheric pressure, is noted by the collected sample gas of pressure difference general who has surrendered Enter and forms sample liquid in buffer solution;
The mechanism for filling liquid (12) is vacantly set to the transmission mechanism (14) upper end with the photoelectric detection system (13), described Card device (15) is examined to be fixedly installed on the transmission mechanism (14), the transmission mechanism (14) drives the inspection card device (15) side-to-side movement, the mechanism for filling liquid (12) realize and sample liquid are injected separately into test point that photoelectric detection system (13) is realized Sample liquid to injecting test point is detected;
The transmission mechanism (14) includes two sliding rails (14.1), a guide rail (14.2), a motor (14.3) and two limits Bit switch (14.4,14.5), detection card device (15) are mounted on two sliding rails (14.1), which exists Guide rail (14.2) can horizontally slip along the guide rail (14.2), and the detection card device (15) is driven by the motor (14.3) Along guide rail side-to-side movement, when the limit switch (14.4) that the shielding plate on the detection card device (15) shelters from left side then stops It moves downward, when the limit switch (14.5) that the shielding plate on the detection card device (15) shelters from right side then stops transporting to the right It is dynamic.
2. full-automatic bioaerosol sampling according to claim 1 and detection robot device, it is characterised in that:It is described Sampling is used to carry out concentration acquisition to air bioaerosol particle with verification unit (2) and category is examined.
3. full-automatic bioaerosol sampling according to claim 1 and detection robot device, it is characterised in that:It is described Traveling unit (1) is for realizing automatic motor function.
4. full-automatic bioaerosol sampling according to claim 1 and detection robot device, it is characterised in that:It is described Video acquisition unit (3) for being monitored in real time to the traveling road conditions of the traveling unit (1).
5. full-automatic bioaerosol sampling according to claim 1 and detection robot device, which is characterized in that described Damper (4) is damping spring.
6. full-automatic bioaerosol sampling according to claim 1 and detection robot device, which is characterized in that described The flow-rate ratio ranging from 1 of main gas circuit and the secondary gas circuit:20-7:200.
7. full-automatic bioaerosol sampling according to claim 1 and detection robot device, which is characterized in that described Buffer solution injection rate is 5ml-25ml.
CN201410757006.3A 2014-12-10 2014-12-10 A kind of full-automatic bioaerosol sampling and detection robot device Active CN105738165B (en)

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