CN105638425B - A kind of water plant co-culture device of adjustable space - Google Patents
A kind of water plant co-culture device of adjustable space Download PDFInfo
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- CN105638425B CN105638425B CN201610030360.5A CN201610030360A CN105638425B CN 105638425 B CN105638425 B CN 105638425B CN 201610030360 A CN201610030360 A CN 201610030360A CN 105638425 B CN105638425 B CN 105638425B
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- stainless steel
- steel grid
- glass
- grid
- water plant
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Cultivation Of Seaweed (AREA)
Abstract
The invention discloses a kind of water plant co-culture devices of adjustable space, including the first stainless steel grid, the second stainless steel grid, third stainless steel grid, co-culture glass jar and glass bar, it is characterized by: glass bar, which is fixed on rectangle with glass cement, co-cultures the first long side surface of glass jar, on the second long side surface, the first stainless steel grid, the second stainless steel grid, third stainless steel grid be any or the equidistant groove for being inserted into glass bar formation in.Present apparatus structure is simple, easy to maintain, low in cost, significantly reduces experimental error, improves accuracy rate, the accuracy of conventional efficient and experiment.Stainless steel material grid has the advantages that tarnish resistance acid and alkali-resistance, high temperature oxidation resisting and the trace level chemical substance for not adsorbing Plant emission in co-culture system, the high-temperature sterilization before can be used for chemical competitive assay and experiment.Grid after experiment can also reuse.
Description
Technical field
The present invention relates to the research fields of chemistry competition between water plant, and chemistry is competing between being more particularly to a kind of water plant
Strive relationship experiments co-culture device.The present apparatus is suitable for water plant co-culture experiments, such as two, three kind of aquatic plant
The experimental design that object coexists convenient for adjusting the volume size of each compartment, efficient isolation experiment object according to experimental subjects, and improves
Co-culture experiments effect.
Background technique
In aquatic ecosystem, between water plant exist in system the life conditions such as space, nutrition and illumination it is competing
The more complex interaction such as strive.The Chemical lab management Competition studied in these water plant inter-species or kind is rich to lake is administered
Nutrient laden, control algal bloom, disclose algae type lake and plant-type lake to mutually convert mechanism significant.In plant species or
The co-cultivation mode of inter-species be study Chemical lab management competition effective means, the design of co-culture device rationally whether be determine grind
Study carefully the primary and key condition of result.
There are two types of the modes that tradition co-cultures: one is two kinds of water plants to cultivate together in same system completely；
Another kind is that two kinds of water plants are separated by partition in same system and cultivate together, and this tradition co-cultures glass jar and exists
Studying during chemical competitive assay has following disadvantage: (1) portion's connection of only keeping on file or the mode to burrow on partition into
It can not completely separated grass when the capable careless algae co-culture experiments for example with submerged plant-phytoplankton (algae) for experimental subjects
And algae, small frustule still can be moved to one side of submerged plant with water flow, be attached on plant leaf blade and grow to it
It has an impact, some filamentous algaes can also be because of physical entanglement interference experiment as a result, influencing the sample acquisition and experimental result of careless algae
Statistical analysis；(2) recycling rate of waterused of co-culture device is poor, and the partition of traditional co-culture device is directly anchored in cylinder,
This feature also limits the plant co-cultivation research that device is used in different volumes size, using limited；(3) partition material list
One is not able to satisfy the needs of kinds of experiments, such as when being related to the experiment of chemical Competition research, co-cultures the partition in cylinder
The trace level chemical substance that Plant emission can be adsorbed, influences experiment effect；(4) fixed-type co-cultivation glass jar cannot basis
The adjustment of experimental subjects volume size co-cultures the space size of compartment；The tradition of (5) two compartments co-cultures glass jar and is not able to satisfy
The co-culture experiments of two or more experimental subjects.
Summary of the invention
In order to solve the above-mentioned problems of the prior art, the object of the present invention is to provide a kind of adjustable spaces
Water plant co-culture device, structure is simple, easy to maintain, low in cost, significantly reduces experimental error, improves experiment
Efficiency and the accuracy rate of experiment, accuracy.The alternative height of the co-culture device is (including different volume of culture ratios and difference
Plant size), co-culturing glass jar than tradition has more high usage and better research effect.Stainless steel material grid
Have the advantages that good acid and alkali-resistance corrosion performance, high temperature oxidation resisting and the trace level chemical substance for not adsorbing co-culture system Plant emission
High-temperature sterilization before can be used for chemical competitive assay and experiment.Grid after experiment can also reuse.
In order to achieve the above purpose, the invention adopts the following technical scheme:
Its technical concept is: the features such as stainless steel grid good toughness, corrosion resistant, strong wearability, is suitable for the invention total training
The characteristics of feeding experimental provision, high temperature oxidation resisting, can make it repeat high-temperature sterilization and use, greatly reduce experiment at
This.And the co-culture device can select different meshes according to the size of algal filament cell in the experiment using algae as object
Stainless steel filter independent assortment inserts in many places groove for co-culturing glass jar, changes compartment space size arbitrarily to meet
The needs of experimental setup are reduced the effect of experiment unfavorable factor interference.
A kind of water plant co-culture device of adjustable space, including the first stainless steel grid, the second stainless steel grid,
Third stainless steel grid co-cultures glass jar and glass bar.It is characterized by: the glass bar of the 4mm thickness of 4mm wide is solid with glass cement
It is scheduled on rectangle and co-cultures the first long side surface of glass jar, on the second long side surface, the spacing of glass bar is 2mm, the first stainless steel lattice
Net, the second stainless steel grid, third stainless steel grid it is any or it is equidistant be inserted into glass bar formation groove in, for large size
The first stainless steel grid, the second stainless steel grid, third stainless steel grid are inserted directly into any groove i.e. when the experiment of frustule
Can, if then can be used glass cement by the first stainless steel grid, the second stainless steel grid, third for the lesser planktonic microalgae of cell
Stainless steel grid is attached in any groove.When co-culturing the experiment of object for two or more, first can be chosen not
Rust steel grid, the second stainless steel grid, any one, two or three in third stainless steel grid come be isolated water plant with
This meets experiment purpose.
The stainless steel grid is used for co-culture experiments, can also extract after experiment.The glass bar
Number can be determined according to the size of the co-cultivation glass jar needed for experiment, then the groove formed is generally advisable at 20 ~ 30,
Both it will not influence the volume of experiment cylinder or can efficiently use.
Compared with prior art, the present invention its advantages and advantage are:
1) groove for co-culturing cylinder is easy to make, and simple glass cylinder may be used for making the device, used in groove
Glass strip material is universal, cheap, and manufacturing process does not have technological difficulties.
2) stainless steel material of grid, which is able to satisfy chemical competitive assay and does not adsorb the trace level chemical substance of Plant emission, wants
It asks, and stainless steel material is easy to clean, convenient for recycling, can also be used for high-temperature sterilization needs before experiment.
3) the characteristics of good toughness of stainless steel grid can be fixed well inserts in a groove, can also be by reality after experiment
The demand of testing extracts, and co-culture device can be used as simple glass cylinder so as to his use at this time, reduces experiment to greatest extent
Cost.
4) design of more grooves be conducive to according to different experiments object (such as two experimental subjects submerged plant-sink water plant
Object, submerged plant-filamentous algae, submerged plant-planktonic microalgae, filamentous algae-planktonic microalgae) volume ratio come insert grid come
Build the compartment in different proportion space；Also it is able to satisfy and chooses two or more experimental setup for co-culturing object.
5) for the chemical competitive assay of different experiments object, experimental subjects to be all higher aquatic plants (as heavy water is planted
Object) when, the grid of gamut mesh number is applicable in.The design can select mesh number, that is, aperture of stainless steel grid according to frustule size
Size, it is proposed that the aperture selection range of grid: 1. large-scale Filamentous algae (10 ~ 70 μm), 200 ~ 1340 mesh grid are applicable in；2. swimming
Microalgae (10 μm or less), 1340 ~ 3000 mesh grid are applicable in.And the filter of the different meshes of selection can be freely combined to reach high
Effect completely cuts off careless algae using grid and macromolecular substances can be made to pass freely through, and co-cultures material exchange in environment and does not hinder.
6) the unfavorable disturbing factor of experiment can be effectively reduced, experimental result accuracy is improved, after experimental study shows transformation
Co-culture glass jar than it is traditional co-culture glass jar parallel laboratory test result error reduce by 80%, favorable reproducibility, and test
Cost reduces 50% or more more originally.
Detailed description of the invention
Fig. 1 is a kind of top view of the water plant co-culture device of the adjustable space of more groove co-cultivation glass jars.
Fig. 2 is a kind of 3 and combines the aquatic plant that stainless steel grid is placed in the adjustable space of more grooves co-cultivation glass jars
The structural schematic diagram of object co-culture device.
In figure: 1- co-cultures glass jar, 2- glass bar, the first stainless steel of 3A- grid, the second stainless steel of 3B- grid, 3C-
Third stainless steel grid, the first long side surface of 1A-, the second long side surface of 1B-.
Specific embodiment
Embodiment 1:
The present invention will now be described in detail with reference to the accompanying drawings.
According to Fig. 1, Fig. 2 it is found that a kind of water plant co-culture device of adjustable space, including the first stainless steel grid
3A, the second stainless steel grid 3B, third stainless steel grid 3C, glass jar 1 and glass bar 2 are co-cultured.It is characterized by: glass bar
2 are fixed on the first of rectangle co-cultivation glass jar 1 with glass cement (the good neutral density glass glue of waterproof seal, market are on sale)
On long side surface 1A, the second long side surface 1B, the first stainless steel grid 3A, the second stainless steel grid 3B, third stainless steel grid 3C appoint
In meaning or the equidistant groove for being inserted into the formation of glass bar 2, the first stainless steel grid 3A when experiment for large-scale frustule, the
Two stainless steel grid 3B, third stainless steel grid 3C are inserted directly into any groove, if for cell it is lesser swim it is micro-
Algae then can be used glass cement to be attached on the first stainless steel grid 3A, the second stainless steel grid 3B, third stainless steel grid 3C arbitrarily
In groove.
It is simple glass that more grooves, which co-culture glass jar 1 and the making material of groove, and more grooves, which co-culture glass jar 1, is
Custom-sized glass jar is needed according to experiment, the glass bar for making groove is also bar shaped glass according to the big little makings of glass jar
Glass material, the size of the cylinder body 1 in this embodiment are 182mm × 140mm × 300mm, the size of glass bar 2 be 4mm × 4mm ×
The spacing of 300mm, glass bar are 2mm, and glass bar 2 has 30, fixes the first stainless steel grid 3A, the second stainless steel with glass cement
Grid 3B, third stainless steel grid 3C, the first stainless steel grid 3A, the second stainless steel grid 3B, third stainless steel grid 3C are cut
At glass jar side (wide × high) size is co-cultured, the first stainless steel grid 3A, the second stainless steel grid 3B, third are stainless
Steel grid 3C is the stainless steel material for not adsorbing the trace level chemical substance of Plant emission.First stainless steel grid in the present embodiment
3A, the second stainless steel grid 3B, third stainless steel grid 3C have been directly inserted in more grooves and have co-cultured position at the three of glass jar 1
It sets.When as chemical competitive assay co-culture device glass jar, the stainless steel grid cut for needing mesh number is inserted into recessed
In slot, the stainless steel grid of different meshes, which can be freely combined, to be inserted in co-culturing the multiple grooves of glass jar, side easy to operate
Just.
The first stainless steel grid 3A, the second stainless steel grid 3B, the third stainless steel grid 3C of different meshes can be combined
It inserts in the groove of many places for co-culturing glass jar 1.
The aperture of grid is selected according to experimental subjects monomer minimum volume (such as frustule) in co-culture experiments to realize
The purpose of co-culture experiments, such as: 1. large-scale Filamentous algae (10 ~ 70 μm), 200 ~ 1340 mesh grid are applicable in；2. planktonic microalgae
(10 μm or less), 1340 ~ 3000 mesh grid are applicable in.
First stainless steel grid 3A, the second stainless steel grid 3B, third stainless steel grid 3C, which can be fixed, is co-culturing glass
It in glass cylinder 1, can also be extracted after experiment, co-culturing glass jar 1 at this time can be used as simple glass cylinder, stainless steel grid 3
High-temperature sterilization before can be used for testing, and it is reusable.
Completely cut off tested experimental subjects and water body material exchange nothing serious purpose to reach to co-culture, then needing to meet difference
Different filamentous algae cell sizes in co-culture experiments, the stainless steel grid of commercial type have that different meshes are available, side
Easy.
The application method of the water plant co-culture device of spatial scalability provided by the invention following (submerged plant and silk
For shape algae, planktonic microalgae co-culture):
Before experiment, glass jar is co-cultured to more grooves and stainless (steel) wire carries out high-temperature sterilization disinfection, the laggard luggage that sterilizes is set
Assembling, configured culture solution is put into glass jar.(1) two kind of co-culture experiments object: and then by the heavy water of pre-treatment
Plant and filamentous algae (or planktonic microalgae) are respectively placed in two compartments (1:1 or 2:1) that glass jar is separated out with grid；(2) three
Kind co-culture experiments object: two grid build the compartment of 3 1:1:1, and plant treatment is same as above.Experimental provision is put into greenhouse again
It is interior, carry out Preparatory work of experiment and the measurement of next step.The more grooves for not doing co-culture experiments group, which co-culture glass jar, also can be used for planting
The control experiment that object is individually cultivated.
It, can after the end of the experiment, by cleaned stainless steel grid nothing to guarantee that the stainless steel grid can reuse
Water-ethanol impregnates or so half an hour, after wiped clean, air-dries and saves, prepare for subsequent experimental.
Claims (2)
1. a kind of water plant co-culture device of adjustable space, including the first stainless steel grid (3A), the second stainless steel lattice
Net (3B), co-cultures glass jar (1) and glass bar (2) at third stainless steel grid (3C), it is characterised in that: glass bar (2) uses glass
Glass glue is fixed on rectangle and co-cultures first long side surface (1A) of glass jar (1), on the second long side surface (1B), the first stainless steel grid
(3A), the second stainless steel grid (3B), third stainless steel grid (3C) are any or equidistant are inserted into the recessed of glass bar (2) formation
In slot, the glass jar is separated into multiple water plant matrix accommodating cavities；The co-cultivation glass jar (1) and glass bar
(2) making material is glass, fixes the first stainless steel grid (3A), the second stainless steel grid (3B), third not with glass cement
It becomes rusty steel grid (3C)；The first stainless steel grid (3A), the second stainless steel grid (3B), third stainless steel grid (3C) are cut out
It is cut into and co-cultures glass jar lateral dimension size, the first stainless steel grid (3A), the second stainless steel grid (3B), third stainless steel
Grid (3C) is stainless steel material；According to the stainless steel grid, adjacent water plant matrix partial size selects lattice in the following manner
Screen distance size and mounting means: the water plant matrix partial size of the stainless steel grid either side is all larger than large-scale filamentous algae
When, select 200~1340 mesh stainless steel grid；It swims when the water plant matrix partial size of the stainless steel grid either side is less than
When algae, 1340~3000 mesh stainless steel grid are selected.
2. a kind of application of the water plant co-culture device of adjustable space in research water plant Chemical lab management competition.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610030360.5A CN105638425B (en) | 2016-01-18 | 2016-01-18 | A kind of water plant co-culture device of adjustable space |
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| CN201610030360.5A CN105638425B (en) | 2016-01-18 | 2016-01-18 | A kind of water plant co-culture device of adjustable space |
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| CN105638425A CN105638425A (en) | 2016-06-08 |
| CN105638425B true CN105638425B (en) | 2019-02-26 |
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| CN107354098A (en) * | 2017-08-08 | 2017-11-17 | 江苏大学 | A kind of common harvesting method of microalgae cultivated using filamentous algae when similar |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2631210A1 (en) * | 1988-05-10 | 1989-11-17 | Cosserat Francois | Growing dish intended to be hung particularly from a bucket chain and bucket chains provided with such dishes |
| CN201199836Y (en) * | 2008-05-20 | 2009-03-04 | 庞淑敏 | Field planting groove for fog culture of white potato |
| CN201726724U (en) * | 2010-04-19 | 2011-02-02 | 寿光沃润德农业科技有限公司 | Soilless culture planting box |
| US20110258920A1 (en) * | 2011-05-31 | 2011-10-27 | Licamele Jason D | V-Trough Photobioreactor System and Method of Use |
| CN202218537U (en) * | 2011-06-02 | 2012-05-16 | 张国英 | Hook blanket type plant basal disc |
| CN102577922A (en) * | 2011-12-30 | 2012-07-18 | 北京工业大学 | Submerged plant and algae coculture equipment |
| CN203346379U (en) * | 2013-06-27 | 2013-12-18 | 广东省微生物研究所 | Simple bacterial biofilm generation device |
| CN205337121U (en) * | 2016-01-18 | 2016-06-29 | 中国科学院水生生物研究所 | Aquatic plant culture apparatus with adjustable space |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203814326U (en) * | 2013-12-27 | 2014-09-10 | 中国水稻研究所 | Rice planting box |
-
2016
- 2016-01-18 CN CN201610030360.5A patent/CN105638425B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2631210A1 (en) * | 1988-05-10 | 1989-11-17 | Cosserat Francois | Growing dish intended to be hung particularly from a bucket chain and bucket chains provided with such dishes |
| CN201199836Y (en) * | 2008-05-20 | 2009-03-04 | 庞淑敏 | Field planting groove for fog culture of white potato |
| CN201726724U (en) * | 2010-04-19 | 2011-02-02 | 寿光沃润德农业科技有限公司 | Soilless culture planting box |
| US20110258920A1 (en) * | 2011-05-31 | 2011-10-27 | Licamele Jason D | V-Trough Photobioreactor System and Method of Use |
| CN202218537U (en) * | 2011-06-02 | 2012-05-16 | 张国英 | Hook blanket type plant basal disc |
| CN102577922A (en) * | 2011-12-30 | 2012-07-18 | 北京工业大学 | Submerged plant and algae coculture equipment |
| CN203346379U (en) * | 2013-06-27 | 2013-12-18 | 广东省微生物研究所 | Simple bacterial biofilm generation device |
| CN205337121U (en) * | 2016-01-18 | 2016-06-29 | 中国科学院水生生物研究所 | Aquatic plant culture apparatus with adjustable space |
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| CN105638425A (en) | 2016-06-08 |
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