CN105628947B - Whole blood automatic luminous immunoassay instant detector - Google Patents
Whole blood automatic luminous immunoassay instant detector Download PDFInfo
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- CN105628947B CN105628947B CN201610071335.1A CN201610071335A CN105628947B CN 105628947 B CN105628947 B CN 105628947B CN 201610071335 A CN201610071335 A CN 201610071335A CN 105628947 B CN105628947 B CN 105628947B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
Abstract
A kind of whole blood automatic luminous immunoassay instant detector disclosed by the invention, it sequentially includes：Integrated Reagent Tube storage takes device, automatic sampling apparatus, project team's parallel reaction processing unit, washing and addition substrate device and photon detection counter；The present invention is using project team's parallel reaction processing unit, and test event is run, detected parallel in the way of project integrated card needed for making any patient, fast report, and detection efficiency is improved；Using intelligentized automatic sample, enable each index concurrent testing of project team, save loading time；Press filtration, washing improve detection efficiency using operation needed for detecting in place, automatically processing each integrated reagent of project team；Pulsed injection washing lotion during present invention washing, continuous pump drainage waste liquid, washing is quick, improves the complete machine speed of service；Apparatus of the present invention coordinate each other with integrated reagent, can well washed corpuscles and be used for the instant detection of whole blood sample, be adapted to the sample such as whole blood, serum, blood plasma and other body fluid.
Description
Technical field
The present invention relates to a kind of whole blood automatic luminous immunoassay instant detector, it is specifically a kind of can be in some stackings
Automatically multiple integrated Reagent Tubes are detected in tray rack and are detected with the luminescence immunoassay for the whole blood sample project team for constituting multi objective
Equipment, and the equipment can improve detection efficiency to the whole blood sample project team parallel reaction processing of some multi objectives.
Background technology
Chemiluminescence technique is because of its high sensitivity, the wide line analyst coverage, good stability, and can automate behaviour
Make, be the outstanding analysis tool of a class.It is combined, constitutes with high specific, highly sensitive heterogeneous immunoassay technology
One class high specific, highly sensitive analysis platform, are widely used in modern medicine, life science.
At present, luminescence immunoassay technology trends are intended to set up personalized reagent, instrument integrated detection side
Case, by the two height fusion to form unique advantage, plays the convenience in use and realizes optimum performance, reach best inspection
Survey result.
In clinical medical inspection field, the developing trend of detection method can be obtained in when doctor, patient's meeting are medical
Accurate assay, i.e., so-called instant detection（Point of Care Test, POCT）Method.It is energy that such method one, which is required greatly,
Whole blood sample is enough detected, therefore reagent considers it is necessary with instrument integrated, to exclude haemocyte composition to testing result
Interference.
In instant detection process, parallel parsing and report multi objective simultaneously are extremely important.Multi objective can be provided more for doctor
Many, more accurately diagnostic message, correct diagnosis is made for doctor；Multi objective parallel parsing can preferably meet detection need immediately
Will.During actually detected, test event, the quantity of each patient are differed；The project team that each patient is detected as
One unit must to this can synchronization process in groups, complete reaction, this is extremely important for detecting immediately.
In POCT class detecting systems, test event, the quantity of each patient are differed.How to realize that integrated reagent exists
Seat in the plane is stored takes with convenient, realizes Synchronization Analysis, for realizing that instant detection is extremely important.The integrated Reagent Tube of crawl is deposited by hand
The risk misapplied in mispick；When specimen amount is big, because operating personnel are in a rush, the risk gone wrong is more added.Cause
This, integrated Reagent Tube is taken very necessary in machine storage and intelligence, automatic quick.
Existing full-automatic immune detection instrument has two kinds to take pipe mode：The general automatic tube cleaner sub-argument of pipe and array crawl are straight
Vertical response pipe.Because the two reaction tube is open general detection pipe, without other outer packings, take pipe process relatively easy.
Denmark's thunder degree AQT90 FLEX immunity analysis instruments, France Mei Liai BioMerieux Vidas, Mitsubishi
It is an instant detection means of class whole blood sample to learn Mitsubishi PATHFAST.This kind of device is used for specified disease and detects limited
Projects combo is tested., can only be after one group of index test be finished due to machine limited bits, then start project team's survey of other patients
Examination.One group of test is usually 16~20 minutes, and the stand-by period is long, and efficiency is low, full-page proof when being unfavorable for outpatient service etc. compared with multisample
Processing.It is more difficult to meet any combination project team detection needs of a variety of disease many indexes.
It is that a current major class detects product immediately using nitrocellulose filter as the lateral immunochromatography technique product of solid phase.It is easy
The characteristics of being its protrusion.However, because material variation is big, process controllability is poor, detection process can not be washed, and product quality is controllable
Property is poor.So that such product sensitivity is low, precision is low, poor repeatability, and dosing accuracy is not enough, is only used for people at highest risk
Primary dcreening operation, it is impossible to the conventional tool as medical diagnosis on disease.It is this by label lateral chromatography product of collaurum and fluorescent material
The representative of class technology.
The multi-target analysis set up with lateral chromatography analytical technology, a kind of method is independently to refer to corresponding project team
Reagent pipetting volume is marked, is stood after the reaction regular hour, its related signal value is read respectively, each index concentration value is calculated, belongs to
Scatter operation, standardization level is low.Another multi objective Parallel analytic method, which is that tested project team's index sample introduction end is centripetal, to be concentrated
In after sample introduction zone a, loading, sample spreads to each index sample introduction end respectively, until completing detection.Later approach is due to each
The difference of reagent sample introduction and agent structure, it is difficult to determine the accuracy of its sample introduction.
Immunex systems under U.S.'s Angen house flags are another multi objective parallel parsing system, this analysis system
It is that the disposable sample for adding certain volume is mixed therewith after mixing multi objective solid phase microballoon and label, the timing of reaction one
Between after washing microballoon.Microballoon after having washed enters microchannel, and the item representated by it is recognized by the single microsphere fluorescence information of counting
Mesh, then the fluorescent marker information content of reactant thereon is counted, so as to realize the analysis detection to multi objective.The method is to inspection
Survey instrument requirements high, and testing efficiency is low.There is certain use value for the scientific research field of micro-example, but face large batch of
Bed examines high flux advantage in work to be difficult to show.
The content of the invention
To solve the above problems, the present invention is directed to propose a kind of whole blood automatic luminous immunoassay instant detector, with fast
Speed is combined to a variety of diseases, any project team of multi objective, parallel detection immediately, is improved detection efficiency and is ensured the spirit of assay
Sensitivity and accuracy.
For up to above-mentioned purpose, the present invention provides a kind of whole blood automatic luminous immunoassay instant detector, including photon is visited
Survey counting device, it is characterised in that it also sequentially includes：
Integrated Reagent Tube storage takes device, and it includes being used to be laminated placing a stand of some pallets, for any one layer
Pallet release or draw in stand a building floor choosing device and one be used for release stand pallet in some integrated Reagent Tubes
The three-dimensional localization pick-up unit taken out is positioned, is loaded the integrated Reagent Tube taken out from pallet by three-dimensional localization pick-up unit
In project integrated card；Described integrated Reagent Tube is made up of micro-pipe and the filter cover being socketed in micro-pipe；
Automatic sampling apparatus, its can the sampling probe that is mounted thereon of three-dimensional running fix, the sampling probe can be in sample cell
The sample liquid fast transfer of absorption is loaded into the integrated Reagent Tube in project integrated card；
Project team's parallel reaction processing unit, it include be on a rotating disk driven by sequential stepping motor rotating shaft, rotating disk
Some necks for being available for project integrated card to be laterally caught in or take out are distributed with radially, are rotated clockwise in rotating disk on direction also
The measuring samples press filtration to project integrated card is sequentially arranged at intervals the first pressue device of source of the gas is provided, project integrated card is provided
One liquid-adding device of reaction solution, a pair of reaction solution press filtrations provide the second pressue device of source of the gas, one for integrated Reagent Tube
Filter cover removes the capping device abandoned；
Described capping device includes the swing rod of a L-shaped, and the cross bar end of swing rod installs a pair and presss from both sides motor-driven by embracing
Folder is embraced, the montant portion end connection one of swing rod drives the montant portion of the swing rod above the rotation motor that swing rod is rotated, rotation motor
The one capping motor that swing rod can be driven to lift also is installed；
Wash mill, washing head thereon by micro-pipe in project integrated card below to being washed and waste liquid being extracted out micro-
Pipe；
Luminous substrate filling apparatus, liquid feeding pin thereon by micro-pipe in project integrated card below to being filled into luminous
Substrate solution；
Photon detection counting device, the photomultiplier under it is visited to micro-pipe in the project integrated card of load luminous substrate liquid
Light-metering subnumber, photon detection counting device can be converted into relative luminous intensity number of photons is added up in the unit interval in micro-pipe
RLU numerical value.
It is fixed with from top to bottom in the filter cover of described integrated Reagent Tube and folds what is constituted by sample filter membrane and label film layer
One combines film.Determinand obtains electrochemiluminescent immunoassay detection by being reacted again with reference to the micro, slight pipe that advance solid phase is instilled after film initial reaction
Use object reaction chain.
Described building floor choosing device is located at behind stand, and building floor choosing device includes the first column and lifting is socketed on the first column
First slide, one is installed in first slide with the first column cooperation to drive that first slide is lifted on the first column first
A mechanism case is fixed in motor, first slide side, and mechanism case installs second electricity on mechanism case towards stand horizontal direction
The expansion link of machine driving, the expansion link is used to release or draw in stand by described pallet.Slided by the first motor driving first
Seat lifting, any one tray position for making mechanism case be elevated on expansion link sensing stand, passes through the second motor and drives expansion link
Stretch out and tray both slid is released before stand, three-dimensional localization pick-up unit integrated Reagent Tube can be examined from pallet and pick up integrated item
In mesh card, the detection project of a sufferer is constituted；Reflection the second motor of driving, you can pallet is withdrawn into by expansion link by stand
It is upper to reset.
Described three-dimensional localization pick-up unit is located at before stand, and three-dimensional localization pick-up unit includes the second column and lifting
It is socketed in the 3rd electricity that a driving second slide is installed in the second slide on the second column, second slide along the second column up-down
Machine, socket one on the first level slide rail parallel with stand or so, first level slide rail is provided with second slide side can left and right
One is installed on the 3rd slide slided, the 3rd slide to coordinate to drive the 3rd slide in first level slide rail with first level slide rail
4th motor of upper slip, second horizontal slide rail parallel with before and after stand, the second horizontal slide rail are provided with the 3rd slide side
One the 4th slide of upper socket, be provided with the 4th slide coordinate with the second horizontal slide rail with drive that the 4th slide slides back and forth the
Five motors；One is fixed below 4th slide is used for the grappling fixture of the integrated Reagent Tube of pick-and-place.Second slide edge is driven by the 3rd motor
Second column is slided up and down, and the 4th motor drives the 3rd slide to be horizontally slipped along first level slide rail, the 5th motor driving the 4th
Slide slides back and forth along the second horizontal slide rail, realizes the grappling fixture being fixed on below the 4th slide in the three dimensions in front of stand
Running fix, integrated Reagent Tube on the pallet of any one layer of release stand is picked out to make an inspection tour inspection, and is picked up by grappling fixture examining
Some integrated Reagent Tubes gone out are put into constitute a detection project in project integrated card, in this way three-dimensional of the invention
Positioning pick-up unit can continuously be put into integrated Reagent Tube in project integrated card one by one, to constitute the detection project of one group of group.
Described automatic sampling apparatus, it includes first cunning of the horizontal first crossbeam and sliding installation on first crossbeam
The second sliding block of block, the second cross beam of level and sliding installation on second cross beam, second cross beam perpendicular to first crossbeam and with
First sliding block is affixed, and described sampling probe is retractably installed at the second slider bottom, by be fixed on the first sliding block
Six motors drive the first sliding block to be slid back and forth along first crossbeam, are slided by the 7th motor driving second being fixed on the second sliding block
Block horizontally slips along second cross beam；Second sliding block side, which is fixed with the 8th motor, to be used to drive sampling probe in the second slider bottom liter
Drop.6th motor drives the first sliding block to be slid back and forth on first crossbeam, and second cross beam is movable with the first sliding block, Ran Houtong
Crossing the 7th motor drives the second sliding block to be horizontally slipped along second cross beam, makes the sampling probe of the second slider bottom can be in two-dimensional level face
Interior movement, then passes through the 8th motor driving sampling needle lifting, you can realize sampling probe from being moved to sample device pipe dish arbitrarily
Device pipe position, is drawn by sampling probe and risen after sample liquid, is then moved in project integrated card above integrated Reagent Tube, decline takes
Sample pin discharges sample liquid into the filter cover of integrated Reagent Tube；In this way can be continuously to the integrated reagent of multiple project integrated cards
Pipe is loaded into sample liquid.
When needing the filter cover of integrated Reagent Tube to be separated with micro-pipe, the cross bar of swing rod is transferred to by rotation motor and turned
Alignd above neck in disk, swing rod height is then reduced by capping motor, make to embrace integrated examination in folder alignment project integrated card
On the outside of the filter cover of agent pipe, then embrace a folder by embracing the driving of folder motor and clamp filter cover, capping motor driving swing rod rise filter cover with it is micro-
Pipe is separated, and swing rod is then screwed out rotating disk by rotation motor, is made to embrace the filter cover discarding position that folder is moved out to outside rotating disk, is then passed through
Embrace the driving of folder motor and embrace folder release, filter cover is dropped under self gravitation in the discard position of filter cover.
The side of described wash mill is installed to be additionally provided with the top of the 9th motor for driving washing head lifting, wash mill
One enters the liquid inlet joint of washing head and a drawing liquid joint for supplying washing head to aspirate discharge waste liquid for cleaning solution；Liquid inlet joint
Feed liquor mode is the pulsed feed liquor that hydraulic pressure and washing time are interrupted.When the integrated card of project is by below wash mill, the 9th
Motor driving washing head lifting stretches into one by one in the micro-pipe of integrated Reagent Tube, then by liquid inlet joint to cleaning solution with pulsed
Micro-pipe is rinsed, and negative pressure is produced by drawing liquid joint, and waste liquid is extracted out by cleaning solution, in this way to each project
Each micro-pipe in integrated card is cleaned.
Described washing head has circumferential on the outer tube communicated with liquid inlet joint and the inner tube communicated with drawing liquid joint, outer tube
With some spray holes for being used to rinse micro-pipe inwall；Spray hole on the outer tube of washing head is carried out to the micro-pipe of integrated Reagent Tube
Waste liquid after flushing, is then pumped out micro-pipe by surrounding and bottom irrigation by inner tube.
A luminous bottom liquid feed liquor pipe joint communicated with liquid feeding pin is additionally provided with the top of described luminous substrate filling apparatus.When
When each project integrated card passes through luminous substrate filling apparatus, plus substrate pin promotes the integrated reagent card movement of project team in bottom
When, luminous substrate is added to integrated reagent cup one by one.
Described photon detection counting device is arranged on in the enterable darkroom of project integrated card.To avoid extraneous light
Influence to photon detection counting device, photon detection counting device is arranged in darkroom, when the integrated card of project passes through darkroom
When, the photomultiplier of photon detection counting device detects number of photons to micro-pipe in the project integrated card of load luminous substrate liquid,
Photon detection counting device can be converted into relative luminous intensity RLU numerical value number of photons is added up in the unit interval in micro-pipe, with
Indicate to participate in the reactant quantity of immune response, by the calibration object curve ratio with storage compared with drawing measured object concentration in sample.
Beneficial effects of the present invention are as follows：
1. the present invention is using project team's parallel reaction processing unit, and test event is with project integrated card needed for making any patient
Mode run, detect that detection efficiency is greatly improved in fast report parallel；
2. intelligentized automatic sample is used, enables each index concurrent testing of project team, saves loading time, simultaneously
It ensure that the accuracy of sample-adding；
3. press filtration, washing in rotating disk uses detection, intelligent control in place, automatically processes each integrated reagent place of project team
Operation is needed, it is efficient, controllable, reliable, improve detection speed；
4. continuous pump drainage waste liquid during present invention washing, pulsed injection washing lotion, are washed quickly, thoroughly, when shortening washing
Between, improve the complete machine speed of service；
5. apparatus of the present invention coordinate each other with integrated reagent, can well washed corpuscles and be used for the instant of whole blood sample
Detection, is adapted to the samples such as whole blood, serum, blood plasma and other body fluid.
Brief description of the drawings
Fig. 1 is the block diagram of complete machine of the present invention（It is distributed by automatic detection flow）.
Fig. 2 takes the dimensional structure diagram of device for the integrated Reagent Tube storage of the present invention.
Fig. 3 is the structure sectional view of the integrated Reagent Tube of the present invention.
Fig. 4 is the dimensional structure diagram of the automatic sampling apparatus of the present invention.
Fig. 5 is the dimensional structure diagram of project team's parallel reaction processing unit of the present invention.
Fig. 6 is the dimensional structure diagram of the capping device of the present invention.
Fig. 7 is the washing of the micro-pipe of the integrated Reagent Tube of the present invention and the structural representation of addition luminous substrate liquid.
Fig. 8 carries out photon detection for the photon detection counting device in the darkroom of the present invention to the micro-pipe in project integrated card
Structural representation.
Accompanying drawing is designated in figure：10. integrated Reagent Tube storage takes device；11. stand；111. pallet；1110. pallet is released
Position；12. building floor choosing device；121. the first column；122. first slide；123. the first motor；124. mechanism case；125. expansion link；
126. the second motor；13. three-dimensional localization pick-up unit；131. the second column；132. second slide；133. the 3rd motor；134.
First level slide rail；135. the 3rd slide；136. the 4th motor；137. the second horizontal slide rail；138. the 4th slide；1381. grab
Head；139. the 5th motor；14. project integrated card；15. integrated Reagent Tube；151. micro-pipe；152. filter cover；153. combine film；154.
Sample filter membrane；155. label films；20. automatic sampling apparatus；21. first crossbeam；22. the first sliding block；23. the 6th motor；24.
Second cross beam；25. the second sliding block；26. the 7th motor；27. sampling probe；28. the 8th motor；29. sample cell；291. sample cell
Disk；30. project team's parallel reaction processing unit；31. rotating shaft；32. rotating disk；33. neck；34. the first pressue device；35. liquid feeding
Device；36. the second pressue device；37. capping device；371. rotation motors；372. capping motors；373. swing rod；374. armfuls of folders；
375. armfuls of folder motors；40. washing and addition substrate device；41. wash mill；411. washing head；4111. outer tube；4112. it is interior
Pipe；4113. spray hole；412. liquid inlet joint；413. drawing liquid joints；414. the 9th motors；42. luminous substrate filling apparatus；
421. liquid feeding pin；422. luminous bottom liquid feed liquor pipe joints；50. photon detection counting device；501. darkroom；51. photomultiplier.
Embodiment
The present invention is further described with reference to the accompanying drawings and examples.
As shown in figure 1, the whole blood automatic luminous immunoassay instant detector of the present invention, it sequentially mainly includes integrated examination
The storage of agent pipe takes device 10, automatic sampling apparatus 20, project team's parallel reaction processing unit 30, washing and the and of addition substrate device 40
Photon detection counter 50；As shown in figure 3, integrated Reagent Tube 15 used in the present invention is by micro-pipe 151 and is socketed in micro-pipe 151
On filter cover 152 constitute, be fixed with from top to bottom in filter cover 152 and be laminated constitute one by sample filter membrane 154 and label film 155
With reference to film 153；The integrated Reagent Tube storage of the present invention take device 10 can rapid batch set up the Item Sets of integrated multiple detection projects
Into card 14, sample liquid is added by the quick each integrated Reagent Tube 15 in each project integrated card 14 of automatic sampling apparatus 20,
Then some groups of parallel reactions of project integrated card 14 are handled by project team's parallel reaction processing unit 30, then passes through washing
And addition substrate device 40 after the micro-pipe 151 of integrated Reagent Tube 15 washing in projects integrated card 14 to adding luminous substrate liquid,
Then photon detection is carried out to the micro-pipe 151 in projects integrated card 14 by photon counting detector 50 to carry out electrochemiluminescent immunoassay point
Analysis.
As depicted in figs. 1 and 2, integrated Reagent Tube of the invention storage takes device 10, including places some pallets for being laminated
111 stand 11, it is used for for any one layer of pallet 111 being released or being drawn in a building floor choosing device 12 and one of stand 11
The three-dimensional localization pick-up unit 13 that some integrated positioning of Reagent Tube 15 in pallet 111 to releasing stand 11 are taken out.The present invention
Building floor choosing device 12 be located at behind stand 11, building floor choosing device 12 includes the first column 121 and lifting and is socketed on the first column 121
First slide 122, install in first slide 122 one and first column 121 coordinate to drive first slide 122 in the first column
A mechanism case 124 is fixed in the first motor 123 lifted on 121, the side of first slide 122, and mechanism case 124 is towards the level of stand 11
The expansion link 125 that second motor 126 on mechanism case 124 drives is installed in direction, and the expansion link 125 is used for described support
Stand 11 is released or drawn in disk 111；Drive first slide 122 to lift by the first motor 123, be elevated to mechanism case 124 and stretch
Contracting bar 125 points to the position of any one pallet 111 on stand 11, drives expansion link 125 to stretch out pallet by the second motor 126
111 slide before release stand 11, pallet 111 is in pallet and release position 1110, the pallet 111 of position 1110 is released positioned at pallet
On integrated Reagent Tube 15 be available for three-dimensional localization pick-up unit 13 inspection pick up.The three-dimensional localization pick-up unit 13 of the present invention is located at stand
Before 11, three-dimensional localization pick-up unit 13 includes the second column 131 and lifts the second slide being socketed on the second column 131
132, the 3rd motor 133 for driving second slide 132 lift along the second column 131 is installed, in the second cunning in second slide 132
132 sides of seat are provided with socket one on the first level slide rail 134 parallel with stand 11 or so, first level slide rail 134 can left and right
One is installed on the 3rd slide 135 slided, the 3rd slide 135 to coordinate to drive the 3rd slide 135 to exist with first level slide rail 134
The 4th motor 136 slided on first level slide rail 134, in the side of the 3rd slide 135 provided with parallel with before and after stand 11 the
It is socketed on one the 4th slide 138, the 4th slide 138 and is provided with and the second water on two horizontal slide rails 137, the second horizontal slide rail 137
Smooth rail 137 coordinates to drive the 5th motor 139 that the 4th slide 138 slides back and forth；The lower section of 4th slide 138, which fixes one, to be used for
The grappling fixture 1381 of the integrated Reagent Tube 15 of pick-and-place；3rd motor 133 driving second slide 132 is slided up and down along the second column 131, the
Four motors 136 drive the 3rd slide 135 to be horizontally slipped along first level slide rail 134, and the 5th motor 139 drives the 4th slide 138
Slid back and forth along the second horizontal slide rail 137, realize the grappling fixture 1381 for being fixed on the lower section of the 4th slide 138 the three of the front of stand 11
Running fix in dimension space, is picked out on the pallet 111 of any one layer of front tray of release stand 11 release position 1110 with making an inspection tour inspection
Integrated Reagent Tube 15, and by grappling fixture 1381 examine some integrated Reagent Tubes 15 picked out be put into project integrated card 14 with
A detection project is constituted, three-dimensional localization pick-up unit 13 of the invention continuously can be put into integrated Reagent Tube 15 in this way
One by one in project integrated card 14, to constitute the detection project of one group of group.Compared with traditional Reagent Tube storage takes device, the present invention
The storage of integrated Reagent Tube take the volume of device 10 smaller, the pallet 111 for storing integrated Reagent Tube 15 is more, three-dimensional localization pick-up unit
Picking and placeing for 13 pairs of integrated Reagent Tubes 15 is more flexible.
As shown in Figure 1 and Figure 4, automatic sampling apparatus 20 of the invention exists including horizontal first crossbeam 21 and sliding installation
The second sliding block of the first sliding block 22, the second cross beam 24 of level and sliding installation on second cross beam 24 on first crossbeam 21
25, second cross beam 24 perpendicular to first crossbeam 21 and with the first sliding block 22 affixed 22, sampling probe 7 is retractably installed at the second cunning
The bottom of block 25, drives the first sliding block 23 to be slided along before and after first crossbeam 21 by the 6th motor 23 being fixed on the first sliding block 22
It is dynamic, drive the second sliding block 25 to be horizontally slipped along second cross beam 24 by the 7th motor 26 being fixed on the second sliding block 25；Second
The side of sliding block 25, which is fixed with the 8th motor 28, to be used to drive sampling probe 27 in the bottom elevation of the second sliding block 25；Can three by the present invention
The dimension running fix sampling probe 21 that is mounted thereon, the sampling probe 27 can be drawing in the sample cell 29 in sample pipe dish 291
On sample liquid fast transfer is loaded into the filter cover 152 of the integrated Reagent Tube 15 in project integrated card 14；In this way may be used
Sample liquid is loaded into the filter cover 152 of the integrated Reagent Tube 15 of continuous multiple project integrated cards 14.
As shown in Figure 1 and Figure 5, project team's parallel reaction processing unit 30 of the invention, it includes one by sequential stepping electricity
The rotating disk 32 that machine rotating shaft 31 drives, radially is distributed with rotating disk 32 and some is available for project integrated card 14 to be laterally caught in or take
The neck 33 gone out, the measuring samples press filtration being also sequentially arranged at intervals on direction to project integrated card 14 is rotated clockwise in rotating disk 32
The first pressue device 34, the liquid-adding device 35 that 14 reaction solutions are provided project integrated card, a pair of the reaction hydraulic pressure that source of the gas is provided
The second pressue device 36, one that filter provides source of the gas is used to the filter cover 152 of integrated Reagent Tube 15 remove the capping device 37 abandoned；
Project to be slided is by the way that the project integrated card 14 of added sample liquid is caught in rotating disk 32 by pushing meanss one by one a group by a group
Neck 33 in, projects integrated card 14 is rotated clockwise with rotating disk 32, rotating disk 32 with the intermittent rotary of sequential machine shaft 31,
When project integrated card 14 moves to the first pressue device 34, the first pressue device 34 is to integrated Reagent Tube in project integrated card 14
Filter cover 152 on 15 is pressurizeed, and testing sample is instilled the bottom of micro-pipe 151 by combining by pushing after the press filtration of film 153, works as project
When integrated card 14 continues to turn to liquid-adding device 35 with rotating disk 32, liquid-adding device 35 is to the note of filter cover 152 on integrated Reagent Tube 15
Enter reaction solution, when the project integrated card 14 for injecting reaction solution moves to the second pressue device 36, the second pressue device 36 is anti-
Answer the initial reactant on hydraulic pressure filter washing combination film 153 and instill the bottom of micro-pipe 151；With consolidating in advance solid phase micro-pipe 151
Phase thing is combined, and sets up reaction chain.It is pulled to after the integrated reagent card standing reaction of project team to the defined time of load sample de-
The lower section of lid arrangement 37.As shown in Figure 5 and Figure 6, capping device 37 of the invention includes the swing rod 373 of a L-shaped, the horizontal stroke of swing rod 373
Bar end installs a pair by embracing armful folder 374 that folder motor 375 drives, and the montant portion end connection one of swing rod 373 drives swing rod 373
The rotation motor 371 of rotation, the montant portion of the swing rod 373 of the top of rotation motor 371, which also installs one, can drive what swing rod 373 was lifted
Capping motor 372；The top of neck 33 that the cross bar of swing rod 373 is transferred in rotating disk 32 is alignd by rotation motor 371, then
The height of swing rod 373 is reduced by capping motor 372, makes to embrace the filter cover that folder 374 is directed at integrated Reagent Tube 15 in project integrated card 14
152 outsides, then filter cover 152 is clamped by embracing the driving armful of folder motor 375 folder 374, the driving swing rod 373 of capping motor 372 rises handle
Filter cover 152 is separated with micro-pipe 151, and swing rod 373 is then screwed out rotating disk 32 by rotation motor 371, makes to embrace folder 374 and be moved out to turn
Filter cover outside disk 32 abandons position, is then unclamped by embracing the driving armful of folder motor 375 folder 374, filter cover 152 is fallen in self gravitation
Enter into the discard position of filter cover, so that the filter cover 152 in project integrated card 14 one by one on integrated Reagent Tube 15 is taken off.Then
Project integrated card 14 with micro-pipe 151 is released from neck 33, into washing and addition luminous substrate link.
As shown in Figure 1 and Figure 7, washing of the invention and addition substrate device 40 are filled by wash mill 41 and luminous substrate
Device 42 is constituted；The side of the wash mill 41 of the present invention is installed by one the 9th motor 414 for driving washing head 411 to lift, washing
The top of device 41 is additionally provided with one and enters the liquid inlet joint 412 and one of washing head 411 for the suction discharge of washing head 411 for cleaning solution
The drawing liquid joint 413 of waste liquid；Washing head 411 has the outer tube 4111 communicated with liquid inlet joint 412 and communicated with drawing liquid joint 413
Inner tube 4112, circumferentially there are on outer tube 4111 some spray holes 4113 for being used to rinse the inwall of micro-pipe 151, liquid inlet joint 412
The pulsed feed liquor that is interrupted for hydraulic pressure and washing time of feed liquor mode；By the movement of washing head 411 to project integrated card 14
In each micro-pipe 151 washed, micro-pipe of the spray hole 4113 on the outer tube 4111 of washing head 411 to integrated Reagent Tube 15
151 carry out surrounding and bottom irrigation, and the waste liquid after flushing is then pumped out micro-pipe 151 by inner tube 4112；Micro-pipe 151 is by washing
The project integrated card 14 washed is sent to the position of luminous substrate filling apparatus 42.The top of luminous substrate filling apparatus 42 of the present invention
A luminous substrate liquid feed liquor pipe joint 422 communicated with liquid feeding pin 421 is additionally provided with, the bottom liquid feed liquor pipe joint 422 that lights is by soft
Pipe is connected to luminous substrate liquid holding bottle, and item below is passed through by 421 pairs of liquid feeding pin on luminous substrate filling apparatus 42
Each micro-pipe 151 is filled into luminous substrate liquid in mesh integrated card 14, produces luminescence-producing reaction；Then by the project of load luminous substrate liquid
Integrated card 14 is sent in the darkroom residing for photon detection counter 50.
As illustrated in figures 1 and 8, the photomultiplier 51 under photon detection counting device 50 of the invention is to the luminous bottom of load
Micro-pipe 151 carries out number of photons detection in the project integrated card 14 of thing liquid, when photon detection counter can be the unit in micro-pipe 151
Interior accumulative number of photons is converted into relative luminous intensity RLU numerical value；Instrument controlling center is set up according to the calibration object curve of storage
RLU- concentration functions, calculate on each project integrated card 14 concentration of each detection project and provide examining report.
The motor activation manners of the present invention also can be using modes such as V belt translation, screw rod transmission, minitype cylinder transmissions.
Above example is used for illustrative purposes only, rather than to present invention limitation, the ordinary skill people of the art
Member, without departing from the spirit and scope of the present invention, can also make various conversion or change, therefore, all equivalent
Technical scheme should also belong to scope of the invention, limited by each claim.
Claims (9)
1. whole blood automatic luminous immunoassay instant detector, including photon detection counter（50）, it is characterised in that its also according to
Sequence includes：
Integrated Reagent Tube storage takes device（10）, it includes being used to be laminated placing some pallets（111）A stand（11）, for handle
Any one layer of pallet（111）Release or draw in stand（11）A building floor choosing device（12）And one be used for release stand（11）
Pallet（111）In some integrated Reagent Tubes（15）Position the three-dimensional localization pick-up unit taken out（13）, pass through three-dimensional localization
Pick-up unit（13）Will be from pallet（111）The integrated Reagent Tube of middle taking-up（15）Loading project integrated card（14）In；Described collection
Into Reagent Tube（15）By micro-pipe（151）Be socketed in micro-pipe（151）On filter cover（152）Constitute；
Automatic sampling apparatus（20）, it can the sampling probe that is mounted thereon of three-dimensional running fix（21）, the sampling probe（27）Can be
Sample cell（29）The sample liquid fast transfer of middle absorption is loaded into project integrated card（14）Interior integrated Reagent Tube（15）In；
Project team's parallel reaction processing unit（30）, it includes one by sequential stepping motor rotating shaft（31）The rotating disk of driving（32）,
Rotating disk（32）On radially be distributed with and some be available for project integrated card（14）The lateral neck for being caught in or taking out（33）, turning
Disk（32）Rotate clockwise and be also sequentially arranged at intervals to project integrated card on direction（14）Measuring samples press filtrations source of the gas is provided
First pressue device（34）, to project integrated card provide（14）One liquid-adding device of reaction solution（35）, a pair of reaction solution press filtrations carry
Second pressue device of air supply source（36）, one be used for integrated Reagent Tube（15）Filter cover（152）Remove the capping device abandoned
（37）；
Described capping device（37）Include the swing rod of a L-shaped（373）, swing rod（373）Cross bar end install a pair by embrace folder
Motor（375）Embracing for driving is pressed from both sides（374）, swing rod（373）Montant portion end connection one drive swing rod（373）The rotation electricity of rotation
Machine（371）, rotation motor（371）The swing rod of top（373）Montant portion also install one and can drive swing rod（373）The capping of lifting
Motor（372）；
Wash mill（41）, washing head thereon（411）To passing through project integrated card below（14）Middle micro-pipe（151）Washing
And waste liquid is extracted out micro-pipe（151）；
Luminous substrate filling apparatus（42）, liquid feeding pin thereon（421）To passing through project integrated card below（14）Middle micro-pipe
（151）Fill into luminous substrate liquid；
Photon detection counting device（50）, the photomultiplier under it（51）To the project integrated card of load luminous substrate liquid（14）
Middle micro-pipe（151）Number of photons is detected, photon detection counter can be micro-pipe（151）Add up number of photons conversion in the interior unit interval
Into relative luminous intensity RLU numerical value.
2. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterised in that：Described integrated examination
Agent pipe（15）Filter cover（152）In be fixed with from top to bottom by sample filter membrane（154）With label film（155）The one of stacking composition
With reference to film（153）.
3. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterised in that：Described choosing layer dress
Put（12）Positioned at stand（11）Below, building floor choosing device（12）Including the first column（121）The first column is socketed in lifting（121）
On first slide（122）, first slide（122）The upper column of installation one and first（121）Coordinate to drive first slide（122）
In the first column（121）First motor of upper lifting（123）, first slide（122）A mechanism case is fixed in side（124）, mechanism
Case（124）Towards stand（11）Horizontal direction installs one by mechanism case（124）On the second motor（126）The expansion link of driving
（125）, the expansion link（125）For by described pallet（111）Release or draw in stand（11）.
4. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterised in that：Described three-dimensional is determined
Position pick-up unit（13）Positioned at stand（11）Above, three-dimensional localization pick-up unit（13）Including the second column（131）With lifting set
It is connected on the second column（131）On second slide（132）, second slide（132）It is upper that a driving second slide is installed（132）Along
Two columns（131）3rd motor of lifting（133）, in second slide（132）Side is provided with and stand（11）Left and right it is parallel
One horizontal slide rail（134）, first level slide rail（134）The 3rd slide that upper socket one can horizontally slip（135）, the 3rd slide
（135）It is upper that one and first level slide rail are installed（134）Coordinate to drive the 3rd slide（135）In first level slide rail（134）On
The 4th motor slided（136）, in the 3rd slide（135）Side is provided with and stand（11）Front and rear the second parallel horizontal slide rail
（137）, the second horizontal slide rail（137）One the 4th slide of upper socket（138）, the 4th slide（138）On be provided with and the second level
Slide rail（137）Coordinate to drive the 4th slide（138）The 5th motor slid back and forth（139）；4th slide（138）Lower section is fixed
One is used for the integrated Reagent Tube of pick-and-place（15）Grappling fixture（1381）.
5. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterised in that：Described enters automatically
Sampling device（20）, it includes horizontal first crossbeam（21）With sliding installation in first crossbeam（21）On the first sliding block（22）、
The second cross beam of level（24）With sliding installation on second cross beam（24）On the second sliding block（25）, second cross beam（24）Vertically
In first crossbeam（21）And with the first sliding block（22）Affixed, described sampling probe（27）It is retractably installed at the second sliding block（25）
Bottom, by being fixed in the first sliding block（22）On the 6th motor（23）Drive the first sliding block（23）Along first crossbeam（21）It is front and rear
Slide, by being fixed in the second sliding block（25）On the 7th motor（26）Drive the second sliding block（25）Along second cross beam（24）Left and right
Slide；Second sliding block（25）Side is fixed with the 8th motor（28）For driving sampling probe（27）In the second sliding block（25）Bottom liter
Drop.
6. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterised in that：Described washing dress
Put（41）Side install one drive washing head（411）9th motor of lifting（414）, wash mill（41）Top is additionally provided with one
Enter washing head for cleaning solution（411）Liquid inlet joint（412）And one supply washing head（411）The drawing liquid of suction discharge waste liquid connects
Head（413）；Liquid inlet joint（412）The pulsed feed liquor that is interrupted for hydraulic pressure and washing time of feed liquor mode.
7. whole blood automatic luminous immunoassay instant detector as claimed in claim 6, it is characterised in that：Described washing head
（411）Have and liquid inlet joint（412）The outer tube communicated（4111）With with drawing liquid joint（413）The inner tube communicated（4112）, outside
Pipe（4111）Upper circumference has some for rinsing micro-pipe（151）The spray hole of inwall（4113）.
8. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterised in that：Described luminous bottom
Thing filling apparatus（42）Top is additionally provided with one and liquid feeding pin（421）The luminous bottom liquid feed liquor pipe joint communicated（422）.
9. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterised in that：Described photon is visited
Survey counting device（50）Installed in for project integrated card（14）Enterable darkroom（501）In.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610071335.1A CN105628947B (en) | 2016-02-02 | 2016-02-02 | Whole blood automatic luminous immunoassay instant detector |
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| CN201610071335.1A CN105628947B (en) | 2016-02-02 | 2016-02-02 | Whole blood automatic luminous immunoassay instant detector |
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| CN105628947B true CN105628947B (en) | 2017-08-08 |
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| CN106124557A (en) * | 2016-06-30 | 2016-11-16 | 芜湖鸣人热能设备有限公司 | The resistance to heat detecting device of hot-water boiler handle |
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| CN106338607B (en) * | 2016-08-30 | 2017-12-29 | 广州蓝勃生物科技有限公司 | A kind of entry detector feeds mechanism for card and feeds chucking method |
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| CN106680479B (en) * | 2017-03-09 | 2024-04-26 | 厦门先明生物技术有限公司 | Multi-index integrated reagent parallel detection arbitrary combination integrator |
| CN108296213A (en) * | 2017-12-26 | 2018-07-20 | 深圳德夏科技发展有限公司 | Board-washing machine |
| CN110736736B (en) * | 2018-07-18 | 2022-10-14 | 上海索昕生物科技有限公司 | Homogeneous phase chemiluminescence POCT detection method and device using same |
| CN110736740A (en) * | 2018-07-18 | 2020-01-31 | 博阳生物科技（上海）有限公司 | homogeneous phase chemiluminescence POCT detection method and device using same |
| CN111257556A (en) * | 2018-11-30 | 2020-06-09 | 深圳市帝迈生物技术有限公司 | Immunoassay analyzer and sample analysis method |
| CN111983249A (en) * | 2020-08-28 | 2020-11-24 | 沈阳瑞好生物科技有限公司 | Chemiluminescence immunodetection analyzer for in-situ luminescence determination and detection method |
| CN112557648B (en) * | 2020-12-25 | 2024-04-16 | 重庆康巨全弘生物科技有限公司 | POCT fluorescence immunoassay appearance |
| CN114113511A (en) * | 2021-12-07 | 2022-03-01 | 南京吉生辉健医疗科技有限公司 | Blood concentration analyzer detecting system |
| CN114112540B (en) * | 2021-12-24 | 2023-08-29 | 蚌埠学院 | On-line rapid water quality residual chlorine analyzer |
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Effective date of registration: 20191118 Address after: Haicang District of Xiamen City, Fujian province 361000 Xinyang Street Weng Kok Road No. 289 building third unit 10 branch Patentee after: XIAMEN XIANMING BIOTECHNOLOGY CO., LTD. Address before: Siming District Yuqing road Xiamen City, Fujian province 361003 No. 33 building B room 1302 Patentee before: Xu Enliang |
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