CN105527279A - Food safety detection apparatus and production method thereof - Google Patents
Food safety detection apparatus and production method thereof Download PDFInfo
- Publication number
- CN105527279A CN105527279A CN201410519427.2A CN201410519427A CN105527279A CN 105527279 A CN105527279 A CN 105527279A CN 201410519427 A CN201410519427 A CN 201410519427A CN 105527279 A CN105527279 A CN 105527279A
- Authority
- CN
- China
- Prior art keywords
- base material
- wood fibre
- reaction zone
- fibre base
- pick
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a food safety detection apparatus and a production method thereof. The detection apparatus comprises a wood fiber base material. A sampling zone and a reaction zone are arranged on the wood fiber base material. At least a chemical detection reagent is provided in the reaction zone. A sample to-be-detected can be absorbed by the sampling zone. The sample to-be-detected moves to the reaction zone on the wood fiber base material, and is subjected to a reaction with the chemical detection reagent. The invention also provides the production method. The apparatus and the method have the advantages of simple operation, safety and rapid analysis.
Description
[technical field]
The present invention relates to a kind of pick-up unit and manufacture method thereof, particularly a kind of pick-up unit for food safety detection and manufacture method thereof.
[background technology]
The importance of food safety detection grows with each passing day, it results from and may remain material food security being existed to doubt in the production run of raw-food material or food, such as many food manufacturers or hotel owner, in order to make food (particularly meat product) fresh-keeping or increase appearance, can with the addition of too much or unsuitable adjuvant (as: color preserving agent, antiseptic etc.) man-hour adding usually; Or after processing, still have the agricultural chemicals remained in food.Above-mentioned additive is for nitrite, when using nitrite as color preserving agent food eaten after in body, the reaction of secondary amine can be produced further and produce there is the N-nitroso compound (N-nitrosocompounds) of highly carcinogenic character in human body.
In simple terms, the certain food additive of excess intake can produce considerable harm to human body.Though relevant decree expressly limits the addition of this type of additive, to make the common people away from too much adjuvant, but, be still difficult to the careless mistake avoided on Food processing, or the illegal activities of illegal dealer.Therefore, how effectively can detect that these food additives were considerable problems before absorption.
The detection method developed for food additives at present comprises spectrophotometric method (spectrophotometry), high-effect liquid chromatography (highperformanceliquidchromatography, HPLC), gas chromatography (gaschromatography, GC), ion chromatography (ionchromatography, IC), capillary electrophoresis (capillaryelectrophoresis, CE), the multiple method such as polarography (polarography) and flow injection analysis (flowinjectionanalysis, FIA).But above-mentioned various analysis must rely on high-end checkout equipment, therefore must expend time in and manpower, can detect under the environment in laboratory.
Health perception along with the common people and the attention for food security improve gradually, and the concept of oneself's detection is gradually prevailing at home.So-called oneself at home detects and refers to that the common people can be in and conveniently to detect at any time, usually can utilize the color transition detecting reagent, easy instrument or do not need the interpretation of instrument naked eyes condition under, the sign with or without disease can be observed immediately.In addition, when aberration becomes large, the further detailed inspection of hospital can be arrived again.Therefore, oneself's detection has in real time convenient and saves the advantage of paying at home.Be applied to the house self-detection method detecting food additives at present and mainly apply colourimetry or photometer ratio juris, and utilize Test paper to add detection reagent to detect.User according to the color of test paper and color table comparison, can get final product the height of contained nitrite concentration in interpretation food.This easy measuring method is bringing great convenience property of the common people and security.Just Test paper still needs to carry especially, and therefore its convenience still has the space of improvement; Moreover existing Test paper is most all through multiple processing, the material added in the process manufactured then likely makes the security of Test paper produce doubt.
Therefore, how a kind of device for detecting safety of foods and manufacture method thereof are provided, it has the ease of Use of test paper, and there is the convenience that mates with articles for daily use and need not additionally carry, and apply special and natural material, to promote security when sampling and the controlling of detection speed and practical application, become one of problem.
[summary of the invention]
Because above-mentioned problem, the object of this invention is to provide a kind of device for detecting safety of foods and manufacture method thereof, it has the ease of Use of test paper, and there is the convenience that mates with articles for daily use and need not additionally carry, and apply special and natural material, to promote security when sampling and the controlling of detection speed and practical application.
For achieving the above object, comprise wood fibre base material according to a kind of device for detecting safety of foods of the present invention.Wood fibre base material is provided with sample region and reaction zone.Reaction zone has at least one chemical detection reagent.Sample to be tested is drawn in sample region.Sample to be tested moves to reaction zone and chemical detection reagent reacting on wood fibre base material.
In one embodiment, wood fibre base material comprises cellulose, lignin and/or hemicellulose.
In one embodiment, pick-up unit is device for detecting safety of foods.
In one embodiment, chemical detection reagent comprises food additives, agricultural chemicals or pathogenic microorganisms detection reagent.
In one embodiment, pick-up unit is stirring rod, toothpick or wooden chopsticks.
In one embodiment, wood fibre base material is provided with further transmission district.Transmit district and connect sample region and reaction zone respectively.Part transmits district through hydrophobic surface treatments.
In one embodiment, reaction zone has accommodation space and block.Accommodation space is formed at a surface of wood fibre base material.Block is placed in accommodation space at least partially, and chemical detection reagent is arranged at block.
In one embodiment, between two parts that the surface that accommodation space is formed at wood fibre base material is separated from each other, and two parts are connected at least in part.
In one embodiment, the component ratio of alpha-cellulose that block comprises is greater than the component ratio of the alpha-cellulose that this reaction zone comprises.
In one embodiment, the density of block is greater than the density of reaction zone.
For achieving the above object, according to the manufacture method of a kind of device for detecting safety of foods of the present invention, comprise the following steps: wood fibre base material is provided; Formation sample region and reaction zone are in wood fibre base material; And at least one chemical detection reagent is set in reaction zone, to form pick-up unit.
In one embodiment, provide the step of wood fibre base material to comprise the material provided containing wood fibre, and isolate wood fibre base material with Physical Processing process.
In one embodiment, wood fibre base material comprises cellulose, lignin and/or hemicellulose.
In one embodiment, chemical detection reagent comprises food additives, agricultural chemicals or pathogenic microorganisms detection reagent.
In one embodiment, pick-up unit is stirring rod, toothpick or wooden chopsticks.
In one embodiment, before providing wood fibre base material step, manufacture method comprises further: shaping wood fibre base material is stirring rod, toothpick or wooden chopsticks.
In one embodiment, manufacture method comprises further: arrange and transmit district in wood fibre base material, transmits district and connects sample region and reaction zone respectively; And transmit district at least partly with hydrophobic surface treatments.
In one embodiment, manufacture method comprises further: form accommodation space and block in reaction zone, accommodation space is formed at a surface of wood fibre base material, and at least one part of block is placed in accommodation space; And chemical detection reagent is set in block.
In one embodiment, between two parts that the surface that accommodation space is formed at wood fibre base material is separated from each other, and two parts are connected at least in part.
In one embodiment, the component ratio of alpha-cellulose that block comprises is greater than the component ratio of the alpha-cellulose that reaction zone comprises.
In one embodiment, the density of block is greater than the density of reaction zone.
From the above, device for detecting safety of foods of the present invention and manufacture method thereof, application includes the reaction zone of chemical detection reagent, effectively to detect specific examination target, such as, is that the nitrite or nitrate received much concern in food security detects.Because device for detecting safety of foods of the present invention includes the structure formed with wood fibre base material, therefore can by wood fibre base material for hydrone preferably adsorbability, thus strengthen the capillary action of sample to be tested in pick-up unit of liquid, promote the speed detected.Moreover, because traditional Test paper needs through additional processing, and may use in process and forbid in food or chemical reagent human body being had to harm, if so directly contact when detecting will the sample to be tested after detecting be caused to eat with sample to be tested; By contrast, because device for detecting safety of foods of the present invention can use natural wood fibre base material, therefore when detecting, detect even can directly contact in partial insertion sample to be tested, and the sample to be tested after detecting still can continue to eat.In addition, also there is low price, the advantage such as easy to process.More preferably, because wood fibre base material has tough physical construction and relative acid and alkali-resistance, therefore the comparable filter paper used traditionally has more the advantage of structural strength aspect.
[accompanying drawing explanation]
Figure 1A is the schematic appearance of a kind of pick-up unit of present pre-ferred embodiments.
Figure 1B is the schematic cross-section of the A-A line shown in Fig. 1.
Fig. 2 is the schematic appearance of the pick-up unit of another embodiment of the present invention.
Fig. 3 is the schematic cross-section of the pick-up unit of another embodiment of the present invention.
Fig. 4 A is the schematic appearance of the pick-up unit of another embodiment of the present invention.
Fig. 4 B is then the schematic cross-section of the B-B line shown in Fig. 4 A.
Fig. 5 A is the schematic appearance of the pick-up unit of another embodiment of the present invention.
Fig. 5 B is then the schematic cross-section of the C-C line shown in Fig. 4 A.
Fig. 6 A is the schematic appearance of the pick-up unit of another embodiment of the present invention.
Fig. 6 B is then the schematic cross-section of the D-D line shown in Fig. 6 A.
Fig. 7 is the manufacture method flow chart of steps of a kind of pick-up unit according to present pre-ferred embodiments.
Fig. 8 A to Fig. 8 C is the result schematic diagram of the pick-up unit detection nitrite shown in application drawing 1.
Fig. 9 is the result schematic diagram of the pick-up unit detection nitrite shown in application drawing 5A.
Figure 10 is detected the result schematic diagram of hydrogen oxide for the pick-up unit shown in application drawing 1.
Figure 11 A and Figure 11 B is the result schematic diagram of the pick-up unit detection pathogenic microorganisms shown in application drawing 1.
[embodiment]
Hereinafter with reference to relevant drawings, a kind of device for detecting safety of foods (hereinafter referred to as pick-up unit) according to present pre-ferred embodiments and manufacture method thereof are described, wherein identical assembly is illustrated with identical reference marks.
Figure 1A is the schematic appearance of a kind of pick-up unit of present pre-ferred embodiments, and Figure 1B is the schematic cross-section of the A-A line shown in Fig. 1.Please refer to shown in Figure 1A and Figure 1B, in the present embodiment, pick-up unit 1 is for sampling and detecting sample to be tested, and sample to be tested is such as biological fluid or food.Though the sample to be tested of the present embodiment is not limited to specific kind, just for clarity of illustration, be that the sample relevant to food security illustrates for sample to be tested in following content, it is such as chaffy dish soup, or for the body fluid (as: saliva) clinically because of the patient of sitotoxismus, with fast and judge its cause of disease clearly.
For promoting the matching with daily living article, pick-up unit 1 can be stirring rod, wooden chopsticks or toothpick, thus can give the extra function of above-mentioned article one (namely detecting); In other words, because above-mentioned article belong to the necessity can carried with in life more, therefore when the pick-up unit of the present embodiment applies these devices, the advantage without the need to additionally carrying detection article can be had more.In the present embodiment, pick-up unit 1 with stirring rod be configured as example explanation.
Please refer to shown in Figure 1A and Figure 1B, the pick-up unit 1 of the present embodiment has wood fibre base material, and in other words, pick-up unit 1 is the structure be made up of wood fibre base material at least partly.When actual manufacture is shaped, pick-up unit 1 preferably entirety all forms with wood fibre base material, certainly, concept of the present invention also contains pick-up unit and is only made up of with wood fibre base material the region partly flowed through for sample to be tested, use the concept formed as " runner ", as shown in Figure 2, pick-up unit 1a has along its axially extended flow passage structure L, flow passage structure L entirety is all formed with wood fibre base material, the material that region beyond flow passage structure L comprises is then the material beyond wood fibre base material, preferably the material that water wettability is lower than wood fibre base material, the present invention does not limit.
With regard to major function composition, the wood fibre base material of pick-up unit 1 is provided with sample region 11, transmits district 12 and reaction zone 13.Wherein, sample region 11, transmission district 12 and reaction zone 13 are sequentially disposed adjacent.On the implementation, the shape in each district, size principle do not limit, and therefore can design according to sample to be tested or examination target, the shape of practical application includes but not limited to cylinder, rectangular parallelepiped, tabular etc., and this does not have restricted to the present invention.
Wherein, alleged " wood fibre base material " means lignified fiber's tissue of plant.Wherein, the lignified tissue that the present invention applies comprises cellulose, hemicellulose, pectic substance and/or lignin in it, and above-mentioned substance has preferably absorption affinity for hydrone; Therefore, sample to be tested via the duct of wood fibre base material inside, sequentially can transport through transmitting 12 to reaction zone, district 13 with capillary action by the sample to be tested from sample region 11.
The material source of the present embodiment pick-up unit 1 can be timber or bamboo wood, goodly can be selected from the comparatively flourishing xylophyta of lignified tissue, as shrub or arbor.Therefore, in practical application with when manufacturing, with the stirring rod made by lignified tissue, wooden chopsticks or toothpick, all the present embodiment pick-up unit 1 can be obtained via reprocessing.Test paper due to traditional food safety detection can be used to forbid when processing process has the chemical reagent of harm (as: utilizing alkali lye to dissolve away lignin) in food or to human body, so directly contact causing the sample to be tested after detecting to eat with sample to be tested when detecting and have safe doubt.By contrast, when the pick-up unit 1 of the present embodiment is applied to and detects food security, because wood fibre base material can be natural material, higher security is had.When detecting, even can directly pick-up unit 1 be applied in partial insertion sample to be tested, and sample to be tested complete after testing need not through extra process (as removing chemical reagent or harmful material), namely capable of direct processing or directly eat.And wood fibre base material is natural material, have more low price, the advantage such as easy to process.
Moreover because wood fibre base material has tough physical construction and relative acid and alkali-resistance, therefore the comparable filter paper used traditionally has more the advantage of structural strength aspect.
Refer again to shown in Figure 1A and Figure 1B, because sample to be tested is sent to reaction zone 13 via the transmission district 12 of pick-up unit 1.Wherein, for filtering detects the impurity in sample, to improve the degree of accuracy detected, can filtering layer (as shown in the dashed region of Figure 1A) be set in sample region 11, just the shape of filtering layer, size and setting position do not have restricted to the present embodiment, demand when detecting according to reality and designing.Such as when the amount of sample to be tested is larger, then can take larger-size filtering layer to improve filter effect.
In the present embodiment, reaction zone 13 is the aspect of groove.Shape, the size of groove do not limit especially, visual detection demand and arranging.And the shape of the groove shown in Figure 1A and Figure 1B is only signal and is not used to limit the present invention.During practical application, the shape of reaction zone 13 can including but not limited to rectangular parallelepiped, square, cylinder, hemisphere, V-type or above-mentioned outside all the other combination of shapes, for Fig. 3, the shape of the reaction zone 13b of pick-up unit 1b is V-type, this structure contributes to the effect of observing its colour developing at sidewall 131b, promotes the accuracy detected.But the position of the reaction zone also demand that detects of visual difference and do elasticity and arrange, the present invention does not limit at this.
Furthermore, for reaching testing goal, number of chemical detects reagent and is arranged in reaction zone 13.Because the pick-up unit 1 of the present embodiment is applied to food safety detection, therefore, the applicable examination target of pick-up unit 1 is such as included in the material that may remain in the production run of raw-food material or food, as food additives, agricultural chemicals, or the pathogenic microorganisms comprised in raw-food material or food.Wherein, the food additives keeping color and luster are usually used in, such as nitrite, due under the environmental baseline of cooking or enter eater oral cavity, nitrite in meat can react with amino acid degradation, generates the nitrosamine having strong carcinogenicity, therefore becomes common examination target.Therefore, the pick-up unit 1 of the present embodiment applies the chemical detection reagent that can react with nitrite ion as examination target, to carry out follow-up analysis and interpretation.
Wherein, alleged by this " setting " one word mean chemical detection reagent to fix or on-fixed in reaction zone 13.Wherein, means chemical detection reagent being fixed on reaction zone 13 include but not limited to that the mode forming covalent bond with the part particular functional base of chemical detection reagent and reaction zone 13 links; Revocable means then can such as apply coating or other similar mode so that chemical detection reagent is arranged in reaction zone 13.
Show in fig. ib, chemical detection reagent is arranged at the bottom of reaction zone 13.But above-mentioned is nonrestrictive, in other embodiments, also chemical detection reagent can be separately set in the side of reaction zone 13 or be arranged at side and the bottom of reaction zone 13 simultaneously.
In addition, the method for reaction zone 13 is adsorbed in about detection reagent, also by reaction zone 13 is immersed in chemical detection reagent solution to complete preparation.Specifically, its capillary action by wood fibre base material and the vascular tissue of vegetable fibre, make chemical detection reagent be adsorbed in reaction zone 13.
Furthermore, when pick-up unit 1 is applied to detection nitrite anions, its chemical detection reagent is selected from P-aminobenzene-sulfonamide (sulfanilamide), citric acid and the combination of N-(1-naphthyl) ethylenediamine (N-(1-naphthyl) ethylenediamine) or the combination of sulfanilic acid (Sulfanilicacid), naphthalidine (1-naphthylamine) and acetic acid.If containing nitrite ion (nitrite) in sample to be tested, then chromogenic reaction can be produced further, to reach qualitative test.And about mode chemical detection reagent the being arranged at reaction zone 13 energy understanding person that is general technical staff of the technical field of the invention, therefore other similar method does not repeat them here.
But above-mentioned application is nonrestrictive, other food additives, comprise bleaching water, water-loss reducer or antiseptic etc., the pick-up unit 1 also can applying the present embodiment detects.Specifically, the bleaching water being used in food is such as hydrogen peroxide (Hydrogenperoxide, H
2o
2) and sodium sulphite (SodiumSulfite).For hydrogen peroxide, though it legally can be used in food (except wheat flour and its products) using the purposes as sterilization, bleaching, must not remain in the final product.Main because the boils up till 152 DEG C of hydrogen peroxide, boil even if therefore boiled by food, hydrogen peroxide still can remain to be existed in food, and long-term eating may cause health hazards.And water-loss reducer, be such as phosphate, though can be used in processed food legally under specified volume restriction equally, but it is not is not checked and approved and is used in fresh meat.Excess ingestion phosphate may cause the imbalance causing human calcium and phosphorus, and causes hyperphosphatemia or hypocalcemia, and then causes non-bone tissue calcification to affect renal function as kidney calcification.In addition, being used in the antiseptic of food, such as, is borax, benzoic acid, though can be used for the food poisoning preventing microorganism from causing, if but excessive or use improperly, the risk that toxicity may be caused even carcinogenic to human body.Therefore, about the food-safety problem that aforesaid food additives may cause, the pick-up unit 1 of the present embodiment can be applicable to play the purposes detecting and check on.The position that its embodiment may exist according to each examination target, such as raw-food material, prescription for a medical decoction, it can be used as sample to be tested, and corresponding each examination target spendable detection reagent is arranged at reaction zone 13 to carry out detection mode as the aforementioned.Similarly, the setting position and the consumption that detect reagent do not have restricted to the present invention, design according to demand during practical application.
In addition, the pick-up unit 1 of the present embodiment also can be applied to detection relevant to residues of pesticides in food security further, such as, synthesize pyrethrosin insecticides (pyrethroidinsecticide) or herbicide.Wherein, synthesis pyrethrosin insecticides comprises go out rather (Cypermethrin), match of match and holds up that peaceful (Cyfluthrin), celo peaceful (lambda-Cyhalothrin), the go out peaceful (Deltamethrin), match phenol peaceful (Cyphenothrin), sweet smellization sharp (Fenvalerate), good fortune profit (Fluvalinate), and herbicide then comprises Paraquat (Paraquat).For above-mentioned agricultural chemicals, the pick-up unit 1 of the present embodiment can provide quick and clearly assay does relevant disposal to allow healthcare givers ahead of time to sufferer.Specifically, when being applied to detection and taking voluntarily or eat the patient of agricultural chemicals by mistake, healthcare givers can apply the process that pick-up unit 1 carries out judging or following the trail of in the process for the treatment of treatment before the treatment.
In addition, the pick-up unit 1 of the present embodiment also can be applied to detection relevant to microorganism in food security further.For milk, only take milk until the process of processing, packaging from ox, or even the manufactured goods after sterilizing, all there is the possibility of bacterial contamination.And the bacterium in milk not only can cause the putrid and deteriorated of milk, more likely can spread disease, harm eater's is healthy.The pick-up unit 1 of the present embodiment can provide food inspection, comprises the residue detected in food, additive or microorganism, concrete as the microbe number detecting milk modification acidizing degree and/or contain.Due to identical in fact with the detection method of previous embodiment about residue, additive or the detection mode of microorganism, and experimental example below will be described in detail, and first not repeat at this.
After completing the arranging of pick-up unit 1, then further pick-up unit 1 can be applied to food safety detection.Its embodiment is as follows, user can carry with pick-up unit 1 and carry out food inspection, and by pick or after sample to be tested is adsorbed in sample region 11 by the mode of fine-still, sample to be tested sequentially along sample region 11, transmit district 12 and be sent to reaction zone 13 with capillary action.If containing nitrite in sample to be tested, aubergine azo-compound can be formed with the chemical reaction reagent in reaction zone 13.That is, whether the pick-up unit 1 of the present embodiment can detect containing nitrite in sample to be tested, to promote the safe diet of individual.Certainly, if need the content of assessment sample to be tested nitrite further to determine whether it exceedes safety value, the pick-up unit 1 of the present embodiment can be reached semiquantitative object in the mode of colourimetry.Preferably, pick-up unit 1 of the present invention can design with the kenel of daily living article, promotes and the matching of daily necessities and the convenience of user.
The above-mentioned kenel about pick-up unit 1 is nonrestrictive.Fig. 4 A is the schematic appearance of the pick-up unit of another embodiment of the present invention, and Fig. 4 B is then the schematic cross-section of the B-B line shown in Fig. 4 A.Please also refer to shown in Fig. 4 A and Fig. 4 B, the pick-up unit 2 of the present embodiment has the structure identical in fact with the pick-up unit 1b of previous embodiment, just the reaction zone 23 of pick-up unit 2 has accommodation space O and block 24, and accommodation space O is formed at a surperficial S of wood fibre base material.In the present embodiment, block 24 is placed in accommodation space O at least partially, and can arrange perpendicular to the long axis direction of pick-up unit 2.Therefore, block 24 can provide sample to be tested to extend the runner of flowing.In actual applications, chemical detection reagent can be arranged at an end face 241 of block 24, and sample to be tested sequentially along sample region 21, transmit district 22 be sent to reaction zone 23 and block 24 with capillary action.
Should be noted that especially, because pick-up unit 2 compares the extra block 24 arranged perpendicular to reaction zone 23 setting direction of pick-up unit 1 of previous embodiment, when watching with sample to be tested overall flow direction, pick-up unit 2 is essentially three-dimensional detection device.
Bamboo wood selected by the wood fibre base material of the present embodiment, and the material selected by block 24 is timber, such as, can select the timber such as birch, poplar or pine, and the density of this timber is preferably the density of the bamboo wood being greater than reaction zone." density " alleged by this refers to the compactness extent of the composition (as: fiber) in timber or bamboo wood.When sample to be tested by reaction zone 23 by capillary flow to block 24 time, its flow velocity can be subject to the impact of density of material and slack-off, the flow conditions making application the present embodiment carry out detecting can be controlled, and the reaction time that sample to be tested can be provided enough, to enable sample to be tested and chemical detection reagent react completely, and then promote the Detection results of pick-up unit 2.Certainly, do not have restricted about the density relation between block 24 and reaction zone 23 to the present embodiment, adjust according to practical application.
And when practical application, the material of block 24 also can select purified alpha-cellulose (α-cellulose).When the component ratio of the alpha-cellulose that block 24 comprises is greater than the component ratio of the alpha-cellulose that wood fibre base material (being preferably reaction zone 23) comprises, because the water wettability of block 24 is good compared with reaction zone 23, therefore, when sample to be tested by reaction zone 23 by capillary flow to block 24 time, its flow velocity can be subject to the impact of water wettability difference and accelerate.In other words, by above-mentioned design, not only extend the conversion zone of sample to be tested by block 24, more can accelerate overall reaction, and then reduce detection time, and there is the advantage reducing sample size.
In addition, about the shape of block 24, the present embodiment is for column, but this is nonrestrictive.When practical application, the shape of block 24 can be cylindrical or tabular, and the structure according to reaction zone 23 is coordinated.
In other embodiments, the set-up mode in extension district also can be as fig. 5 a and fig. 5b.In the present embodiment, pick-up unit 3 has the structure identical in fact with the pick-up unit 1 of previous embodiment, and just its reaction zone 33 is the accommodation space O by being formed in the mode of such as cutting by the part-structure of pick-up unit 3.Wherein, accommodation space O be formed at that the surperficial S-phase of wood fibre base material is separated mutually between two parts S1, S2, and two parts S1, S2 are connected at least in part.By above-mentioned molding mode, the accommodation space O of the present embodiment has the structure of V-type.By being arranged in accommodation space O by block 34, sample to be tested can be provided equally to extend the runner of flowing.And about the material of block 34 and implementation detail identical with previous embodiment haply, therefore not repeat them here.
Should be noted that, the mode of reaction zone 23,33 is arranged at and unrestricted the present invention about block 24,34, it can contact with each other as the principle arranged with block 24,34 and reaction zone 23,33, mode can such as but not limited to being inlayed by block 24,34 or being sticked in reaction zone 23,33, or block 24,34 is attached to reaction zone 24,34 also can.
Furthermore, when block 24,34 is the structure that can be separated with reaction zone 23,33, after completion of reaction, further block 24,34 autoreaction district 23,33 is removed, and carry out other analysis according to its detection demand.
Fig. 6 A is the schematic appearance of the pick-up unit of another embodiment of the present invention, and Fig. 6 B is then the schematic cross-section of the D-D line shown in Fig. 6 A.Please also refer to shown in Fig. 6 A and Fig. 6 B, the pick-up unit 4 of the present embodiment has the structure identical in fact with the pick-up unit 1 of previous embodiment, and surface treatment (as darker regions 45 in Fig. 6 A and Fig. 6 B) is additionally carried out to increase the stability of wood fibre in the transmission district 42 and the reaction zone 43 that are pick-up unit 4.Wherein, surface treatment is such as but not limited to hydrophobic treatments, and hydrophobic agents coats at least part of transmission district 42 and the surface of reaction zone 43 including but not limited to PDMS (dimethyl silicone polymer, Polydimethylsiloxane).Can define and reduce the hydrophilic region transmitting district 42 and reaction zone 43 and comprise by above-mentioned process further, to make sample to be tested can be sent to reaction zone 43 via hydrophilic region exactly, and react with chemical detection reagent.
Mode about hydrophobic treatments is also restrictive.When practical application, also by having painting photoresist layer on hydrophilic pick-up unit 4; Specifically, such as when using SU-8 epoxy radicals negative photoresist (SU-8epoxy-basednegativephotoresist), development of photoresist liquid can not be dissolved in the light-struck region of UV, hydrophobic region can be formed, then do not maintain its hydrophilic characteristic with the light-struck region of UV.Due to the similar generation type energy understanding person that is general technical staff of the technical field of the invention, do not repeat them here.
Fig. 7 is the manufacture method flow chart of steps of a kind of pick-up unit according to present pre-ferred embodiments.Please refer to shown in Fig. 7, in the present embodiment, the manufacture method of pick-up unit comprises following steps: provide wood fibre base material (S71); Formation sample region and reaction zone are in wood fibre base material (S73); At least one chemical detection reagent is set in reaction zone, to form pick-up unit (S75).Wherein, provide the step of wood fibre base material to comprise the material provided containing wood fibre, and isolate this wood fibre base material with Physical Processing process, the mode of Physical Processing does not have restricted to the present invention.In addition, the mode forming sample region and reaction zone such as but not limited to for physically to the destruction that wood fibre base material carries out slightly, to define the position of each subregion.Further, pick-up unit can provide different manufacture methods according to its different enforcement aspect, such as, when pick-up unit toothpick, is tapered off to a point its side by the mode of cutting, to provide the purposes of picking one's teeth simultaneously.Should be noted that especially, the manufacture method that the pick-up unit of the various embodiments described above all can apply the present embodiment is that principle manufactures, and adjusts according to the modular construction of its reality and detection demand.Because each assembly of above-mentioned detection device and each step of manufacture method describe in detail in the aforementioned embodiment, do not repeat them here.
Next practical manner and the effect of pick-up unit 1 of the present invention will be illustrated with experimental example.But it is noted that illustrate that being used to that the present invention is described in detail in detail realizes according to this to enable this have the knack of this operator below, and the pick-up unit can applying other embodiment of the present invention equally carries out, but and be not used to limit scope of the present invention.
Experimental example 1: the test carrying out nitrite with pick-up unit 1
By chemical detection reagent with micropipet (Gilson, Inc.) reaction zone 13 of pick-up unit 1 is dropped to, chemical detection pack is containing P-aminobenzene-sulfonamide (sulfanilamide) (≤99% of 50mmol/L, Sigma-Aldrich), citric acid (≤99.5% of 330mmol/L, Sigma-Aldrich), with 10mmol/LN-(1-naphthyl) ethylenediamine (N-(1-naphthyl) ethylenediamine) (≤98%, Sigma-Aldrich).After completing the interpolation of chemical detection reagent, by pick-up unit 1 at 25 DEG C air-dry 15 minutes.Then sample to be tested is taked with the sample region 11 of pick-up unit 1, the source of sample to be tested is comprise the nitrite standard items (damping fluid group) with deionized water preparation, and add in chaffy dish soup nitrite standard items (spikingtest) (food group), after 7 minutes, the colored intensity carrying out reaction zone 13 with ImageJ image analysis software judges.
Result please refer to Fig. 8 A to Fig. 8 C.After the effect of sample to be tested and chemical detection reagent, detect the mean intensity of its chromogenic reaction, can find out the increase along with nitrite concentration, the mean intensity of its chromogenic reaction increases thereupon.And by shown in Fig. 8 C, the nitrite of more each concentration (damping fluid group) or the test of (food group) in chaffy dish soup in deionized water, the color change of reaction zone equally all deepens along with the increase of nitrite concentration.
Experimental example 2: the test carrying out nitrite with pick-up unit 2
By chemical detection reagent with micropipet (Gilson, Inc.) the extension district 24 of pick-up unit 2 is dropped to, chemical detection pack is containing P-aminobenzene-sulfonamide (sulfanilamide) (≤99% of 50mmol/L, Sigma-Aldrich), citric acid (≤99.5% of 330mmol/L, Sigma-Aldrich), with 10mmol/LN-(1-naphthyl) ethylenediamine (N-(1-naphthyl) ethylenediamine) (≤98%, Sigma-Aldrich).After completing the interpolation of chemical detection reagent, by pick-up unit 1 at 25 DEG C air-dry 15 minutes.Then sample to be tested is taked with the sample region 21 of pick-up unit 2, the source of sample to be tested is comprise each concentration nitrite standard items (0mM, 0.156mM, 0.625mM, 1.25mM) with deionized water preparation, after 7 minutes, the intensity of carrying out reaction zone colour developing with ImageJ image analysis software judges.
Result please refer to shown in Fig. 9.After the effect of sample to be tested and chemical detection reagent, detect the mean intensity of its chromogenic reaction, relatively nitrite concentration is the testing result of 0mM, 0.156mM, 0.625mM, 1.25mM, the increase along with nitrite concentration can be found out, the mean intensity of its chromogenic reaction increases thereupon, and the color change of reaction zone also deepens along with the increase of nitrite concentration.
Experimental example 3: the test carrying out hydrogen peroxide with pick-up unit 1
The chemical detection reagent of 4 μ L potassium iodides (0.6M) is dropped to the reaction zone 13 of pick-up unit 1 with micropipet (Gilson, Inc.).After completing the interpolation of chemical detection reagent, by pick-up unit 1 at 25 DEG C air-dry 10 minutes.Then the sample region 11 of 5 set detecting devices 1 to be immersed respectively in the superoxol of 0%, 0.3%, 0.5%, 1%, 15% 50 seconds, after completing, detect the mean intensity of each group respectively.
Result please refer to shown in Figure 10.After the effect of sample to be tested and chemical detection reagent, detect the mean intensity of its chromogenic reaction, compare the testing result of the hydrogen peroxide of 0%, 0.3%, 0.5%, 1%, 15%, the increase along with concentration of hydrogen peroxide can be found out, the mean intensity of its chromogenic reaction increases thereupon, and the color change of reaction zone also deepens along with the increase of concentration of hydrogen peroxide.
Experimental example 4: the test carrying out pathogenic microorganisms with pick-up unit 1
The sodium hydroxide solution (62.5mM) of 4 μ L is dropped to the reaction zone 13 of pick-up unit 1 with micropipet (Gilson, Inc.).Pick-up unit 1 is transferred to dry 10 minutes at 25 DEG C.Then, 2 μ L resazurin (resazurin) solution (0.4%w/v) are dropped on sodium hydroxide solution, and by pick-up unit 1 at 25 DEG C air-dry 10 minutes.Then being immersed respectively between resting period the sample region 11 of 4 set detecting devices 1 is in the milk of 0,4,8,12 hour 5 minutes, detects the degree of spoilage of each group of milk after completing respectively.
Result please refer to shown in Figure 11 A and Figure 11 B.After the effect of sample to be tested and chemical detection reagent, detect the mean intensity of its chromogenic reaction, compare between resting period the testing result of the milk being 0,4,8,12 hour.Because along with the prolongation between milk resting period, wherein grown microorganism should increase, and even produces corrupt acidifying, and this two factor all can cause the chemical detection reagent containing resazurin solution to produce chromogenic reaction.Really can detect that milk is along with increasing standing time by the pick-up unit 1 of the susceptible of proof embodiment of the present invention in figure, contained micro organism quantity increases, and produces the phenomenon of corrupt acidifying even further.
In sum, device for detecting safety of foods of the present invention and manufacture method thereof, application includes the reaction zone of chemical detection reagent, effectively to detect specific examination target, such as, is that the nitrite or nitrate received much concern in food security detects.Because device for detecting safety of foods of the present invention includes the structure formed with wood fibre base material, therefore can by wood fibre base material for hydrone preferably adsorbability, thus strengthen the capillary action of sample to be tested in pick-up unit of liquid, promote the speed detected.Moreover, because traditional Test paper needs through additional processing, and may use in process and forbid in food or chemical reagent human body being had to harm, if therefore directly contact when detecting will the sample to be tested after detecting be caused to eat with sample to be tested; By contrast, because device for detecting safety of foods of the present invention can use natural wood fibre base material, therefore when detecting, detect even can directly contact in partial insertion sample to be tested, and the sample to be tested after detecting still can continue to eat.In addition, low price, the advantage such as easy to process is had more.More preferably, because wood fibre base material has tough physical construction and relative acid and alkali-resistance, therefore the comparable filter paper used traditionally has more the advantage of structural strength aspect.
The foregoing is only illustrative, instead of restrictive.Anyly do not depart from spirit of the present invention and category, and to its equivalent modifications of carrying out or change, all should comprise in the appended claims.
Claims (20)
1. a device for detecting safety of foods, comprising:
Wood fibre base material, described wood fibre base material is provided with:
Sample region; With
Reaction zone, has at least one chemical detection reagent,
Sample to be tested is drawn in wherein said sample region, and described sample to be tested moves to described reaction zone and described chemical detection reagent reacting on described wood fibre base material.
2. device for detecting safety of foods as claimed in claim 1, wherein said wood fibre base material comprises cellulose, lignin and/or hemicellulose.
3. device for detecting safety of foods as claimed in claim 1, wherein said chemical detection reagent comprises food additives, agricultural chemicals or pathogenic microorganisms and detects reagent.
4. device for detecting safety of foods as claimed in claim 1, it is stirring rod, toothpick or wooden chopsticks.
5. device for detecting safety of foods as claimed in claim 1, described wood fibre base material is provided with further:
Transmit district, connect described sample region and described reaction zone respectively, and the described transmission district of part is through hydrophobic surface treatments.
6. device for detecting safety of foods as claimed in claim 5, wherein said reaction zone has accommodation space and block, described accommodation space is formed at a surface of described wood fibre base material, described block is placed in described accommodation space at least partially, and described chemical detection reagent is arranged at described block.
7. device for detecting safety of foods as claimed in claim 6, between two parts that the described surface that wherein said accommodation space is formed at described wood fibre base material is separated from each other, and described two parts are connected at least in part.
8. device for detecting safety of foods as claimed in claim 6, the component ratio of the alpha-cellulose that wherein said block comprises is greater than the component ratio of the alpha-cellulose that described reaction zone comprises.
9. device for detecting safety of foods as claimed in claim 6, the density of wherein said block is greater than the density of described reaction zone.
10. a manufacture method for device for detecting safety of foods, comprises the following steps:
Wood fibre base material is provided;
Formation sample region and reaction zone are in described wood fibre base material; And
At least one chemical detection reagent is set in described reaction zone, to form described pick-up unit.
11. manufacture methods as claimed in claim 10, wherein provide the step of described wood fibre base material to comprise the material provided containing wood fibre, and isolate described wood fibre base material with Physical Processing process.
12. manufacture methods as claimed in claim 10, wherein said wood fibre base material comprises cellulose, lignin and/or hemicellulose.
13. manufacture methods as claimed in claim 10, wherein said chemical detection reagent comprises food additives, agricultural chemicals or pathogenic microorganisms and detects reagent.
14. manufacture methods as claimed in claim 10, wherein said pick-up unit is stirring rod, toothpick or wooden chopsticks.
15. manufacture methods as claimed in claim 14, before providing described wood fibre base material step, comprise further:
Shaping described wood fibre base material is described stirring rod, described toothpick or described wooden chopsticks.
16. manufacture methods as claimed in claim 10, comprise further:
Arrange and transmit district in described wood fibre base material, described transmission district connects described sample region and described reaction zone respectively; And
With at least part of described transmission district of hydrophobic surface treatments.
17. manufacture methods as claimed in claim 16, comprise further:
Form accommodation space and block in described reaction zone, described accommodation space is formed at a surface of described wood fibre base material, and at least one part of described block is placed in described accommodation space; And
Described chemical detection reagent is set in described block.
18. manufacture methods as claimed in claim 17, between two parts that the described surface that wherein said accommodation space is formed at described wood fibre base material is separated from each other, and described two parts are connected at least in part.
19. manufacture methods as claimed in claim 17, the component ratio of the alpha-cellulose that wherein said block comprises is greater than the component ratio of the alpha-cellulose that described reaction zone comprises.
20. manufacture methods as claimed in claim 17, the density of wherein said block is greater than the density of described reaction zone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410519427.2A CN105527279A (en) | 2014-09-30 | 2014-09-30 | Food safety detection apparatus and production method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410519427.2A CN105527279A (en) | 2014-09-30 | 2014-09-30 | Food safety detection apparatus and production method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105527279A true CN105527279A (en) | 2016-04-27 |
Family
ID=55769637
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410519427.2A Pending CN105527279A (en) | 2014-09-30 | 2014-09-30 | Food safety detection apparatus and production method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105527279A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108931518A (en) * | 2018-05-25 | 2018-12-04 | 洛阳理工学院 | A kind of nitrite ionic selectivity Test paper and the preparation method and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3732147A (en) * | 1970-09-17 | 1973-05-08 | Miles Lab | Colorimetric determination of dehydrogenases |
| CN87108365A (en) * | 1986-12-19 | 1988-08-03 | 曼海姆泊灵格股份公司 | Body fluid components is carried out the test carrier of assay determination |
| WO2002004465A1 (en) * | 2000-07-06 | 2002-01-17 | Fal Diagnostics | Methods and kits for the detection of arginine compounds |
| CN201611336U (en) * | 2010-03-02 | 2010-10-20 | 苏州市玮琪生物科技有限公司 | Hydrogen peroxide detection device |
| TWM420693U (en) * | 2011-07-04 | 2012-01-11 | Sand County Biotechnology Inc | Sensing chip structure with fast guiding reaction effect |
| CN202533438U (en) * | 2011-12-22 | 2012-11-14 | 黄立伟 | Narcotic drug detecting device |
| CN104251857A (en) * | 2013-06-25 | 2014-12-31 | 郑兆珉 | Three-dimensional wood fiber detection tool |
-
2014
- 2014-09-30 CN CN201410519427.2A patent/CN105527279A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3732147A (en) * | 1970-09-17 | 1973-05-08 | Miles Lab | Colorimetric determination of dehydrogenases |
| CN87108365A (en) * | 1986-12-19 | 1988-08-03 | 曼海姆泊灵格股份公司 | Body fluid components is carried out the test carrier of assay determination |
| WO2002004465A1 (en) * | 2000-07-06 | 2002-01-17 | Fal Diagnostics | Methods and kits for the detection of arginine compounds |
| CN201611336U (en) * | 2010-03-02 | 2010-10-20 | 苏州市玮琪生物科技有限公司 | Hydrogen peroxide detection device |
| TWM420693U (en) * | 2011-07-04 | 2012-01-11 | Sand County Biotechnology Inc | Sensing chip structure with fast guiding reaction effect |
| CN202533438U (en) * | 2011-12-22 | 2012-11-14 | 黄立伟 | Narcotic drug detecting device |
| CN104251857A (en) * | 2013-06-25 | 2014-12-31 | 郑兆珉 | Three-dimensional wood fiber detection tool |
Non-Patent Citations (3)
| Title |
|---|
| CHEN-MENG KUAN 等: "Lignocellulose-based analytical devices: bamboo as an analytical platform for chemical detection", 《SCIENTIFIC REPORTS》 * |
| 安提亚·赛安 等: "《101个植物的实验》", 28 February 2011 * |
| 郑兆珉: "Cellulose-based Diagnostic Devices for Public Health and Food Safety", 《台北医学大学生医材料暨组织工程研究所拇上产学论坛讲座 HTTP：//GIBMT.TMU.EDU.TW/APP/NEWS/PHP?SN=36》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108931518A (en) * | 2018-05-25 | 2018-12-04 | 洛阳理工学院 | A kind of nitrite ionic selectivity Test paper and the preparation method and application thereof |
| CN108931518B (en) * | 2018-05-25 | 2021-04-13 | 洛阳理工学院 | Nitrite ion selective detection test paper and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hartzfeld et al. | Determination of hydrolyzable tannins (gallotannins and ellagitannins) after reaction with potassium iodate | |
| Manchali et al. | Nutritional composition and health benefits of various botanical types of melon (Cucumis melo L.) | |
| Tangpao et al. | Evaluation of markers associated with physiological and biochemical traits during storage of ‘Nam Dok Mai Si Thong’mango fruits | |
| CN101315360A (en) | Bioassay method for pest | |
| CN105527279A (en) | Food safety detection apparatus and production method thereof | |
| CN104749175A (en) | Method for detecting organophosphorus pesticide residues in fruits and vegetables | |
| Wang et al. | pH-Responsive Polymer Films Based on Click Polymerization for Food Freshness Monitoring: Non-Toxic, Non-Leaking, and Antibacterial | |
| Choi et al. | Development of a microbial decontamination system combining washing with highly activated calcium oxide solution and antimicrobial coating for improvement of mandarin storability | |
| US9689854B2 (en) | Food safety detection device and manufacturing method for the same | |
| CN105918429A (en) | Method for comprehensively preserving and storing prunus persica at normal temperature | |
| US20140315285A1 (en) | Lignocellulosic detection device | |
| Cattaneo et al. | The aquaphotomics approach as a tool for studying the influence of food coating materials on cheese and winter melon samples | |
| Mukhtar et al. | Some nutritional and microbiological aspects of apples of common varieties available for household consumption. | |
| CN104509762A (en) | Sucking cactus jelly and preparation method thereof | |
| TWI550270B (en) | Food safety detection device and manufacturing method for the same | |
| CN104251857A (en) | Three-dimensional wood fiber detection tool | |
| CN103399012A (en) | Colorimetric sensing paste film and preparation method thereof | |
| CN106283923A (en) | A kind of appendix has mildew-proof wallpaper of medicine carrying chitosan and preparation method thereof | |
| Schirra et al. | Complexation of imazalil with β-cyclodextrin, residue uptake, persistence, and activity against Penicillium decay in citrus fruit following postharvest dip treatments | |
| US9612203B2 (en) | Detection device and manufacturing method for the same | |
| Da Costa et al. | Mineral and natural films change the physical–chemical properties of grapes and modulate oviposition behaviour of Ceratitis capitata Wiedemann (Diptera: Tephritidae) | |
| CN105717101A (en) | Detection device and manufacturing method thereof | |
| Bebek Markovinović et al. | A chemometric investigation on the functional potential in high power ultrasound (HPU) processed strawberry juice made from fruits harvested at two stages of ripeness | |
| Shabangu | The occurrence of fungi and their mycotoxins in maize and bambara nuts and their effect on the health of the rural community in areas of Limpopo province | |
| US20170216486A1 (en) | System and Method for Treating a Wound |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160427 |