CN105505829A - Fermented traditional Chinese medicine dominant bacterial community selective culture medium and preparation method thereof, and screening method of fermented traditional Chinese medicine dominant bacteria - Google Patents
Fermented traditional Chinese medicine dominant bacterial community selective culture medium and preparation method thereof, and screening method of fermented traditional Chinese medicine dominant bacteria Download PDFInfo
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- CN105505829A CN105505829A CN201610037368.4A CN201610037368A CN105505829A CN 105505829 A CN105505829 A CN 105505829A CN 201610037368 A CN201610037368 A CN 201610037368A CN 105505829 A CN105505829 A CN 105505829A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Abstract
The invention relates to a dominant bacterial community selective culture medium and a preparation method thereof, and a screening method of dominant bacteria, particularly a fermented traditional Chinese medicine dominant bacterial community selective culture medium and a preparation method thereof, and a screening method of fermented traditional Chinese medicine dominant bacteria. The culture medium solves the problems of great quality fluctuations and unstable quality in the traditional Chinese medicine fermented product, the problem of great variety and quantity variance in the fermented traditional Chinese medicines separated at present, and the problems of high time and labor consumption and unideal fermentation effect due to repeated separation and purification. The culture medium comprises a traditional Chinese medicine decoction concentrated solution, glucose and agar. The preparation method comprises the following steps: adding the glucose and agar into the traditional Chinese medicine decoction concentrated solution, shaking up, and sterilizing. The culture medium can be used in the field of fermented traditional Chinese medicines. The screening method comprises the following steps: 1. spreading on a plate; and 2. grinding the fermented traditional Chinese medicines, diluting, coating a culture medium in the step 1, and culturing. The screening method is used in the field of fermented traditional Chinese medicines.
Description
Technical field
The present invention relates to the screening method of a kind of dominant microflora selective medium and preparation method thereof and dominant bacteria.
Background technology
Fermentation class Chinese medicine utilizes traditional science of Chinese drug processing fermentation method to utilize the fermentable Chinese medicine in environment or beans, flour etc. to form in natural condition, for multi-cultur es mixing spontaneous fermentation, in order to reach the object improving drug effect, eliminate toxic side effect, change the property of medicine.
Traditional herb fermenting adopts natural microbial multiple bacteria compound fermentation, and bacterial classification is impure, and metabolism is theoretical, effective component theory is smudgy, production poor controllability; In addition different geographical, different production environment, different material, different to produce the microorganism in season variant, thus causes the quality product fluctuation of fermented tcm large, unstable.
In order to obtain solid-state fermentation flora, to improve and to ensure the quality of production of fermentation class Chinese medicine, select the microorganism species in nutrient agar, potato dextrose agar, wort agar substratum separation and Culture fermented tcm at present, these three kinds of substratum are applicable to the cultivation of bacterium, yeast and mold respectively, the different types of flora of culture of isolated that can only be independent; And a kind of substratum can not be adopted separately for the Chinese medicine of multi-cultur es mixing spontaneous fermentation and obtain whole dominant microflora, need multiple substratum be separated different flora, not only waste time and energy, dominant bacteria separating effect is also undesirable.
Summary of the invention
The present invention is large, unstable in order to solve traditional herb fermenting quality product fluctuation, and be separated at present microbe species in the fermented tcm that obtains, bacterial classification quantity variance larger; And also need separating for several times purifying, waste time and energy, the problem that bacterial screening effect is also undesirable, and the screening method of a kind of ferment class important advantages flora selective medium and preparation method thereof and the fermentation class important advantages bacterium that provide.
The present invention's class important advantages flora selective medium that ferments comprises decocting for Chinese herbal medicine concentrated solution, glucose and agar.
Above-mentioned fermentation class important advantages flora selective medium is prepared according to the following steps: glucose and agar are added in decocting for Chinese herbal medicine concentrated solution and shake up, then sterilizing, namely obtains fermentation class important advantages flora selective medium.
Carry out according to the following steps by the method for above-mentioned fermentation class important advantages flora selective medium screening fermentation class important advantages bacterium:
One, fermentation class important advantages flora selective medium is paved plate, then naturally cooling solidifies;
Two, fermentation class Chinese medicine is aseptically ground by the stroke-physiological saline solution that concentration is 0.9%, and then dilution is applied to step one and ferments on class important advantages flora selective medium, be placed in 20 DEG C ~ 40 DEG C to cultivate 2 ~ 7 days, namely obtain this fermentation class important advantages flora.
The present invention's class important advantages flora selective medium that ferments is by force pointed, can meet the growth and breeding needs of all dominant microfloras in fermented tcm completely, can not cause the disappearance of fermentation dominant microflora; And the selectivity of substratum of the present invention is good, dominant microflora can grow vigorous during the fermentation, and decocting for Chinese herbal medicine concentrated solution effectively can suppress the growth of miscellaneous bacteria, thus avoids interference and the impact of miscellaneous bacteria.
Adopt the present invention's dominant microflora of class important advantages flora selective medium to fermentation class Chinese medicine that ferment to carry out screening and have advantage fast and accurately, the dominant bacteria being particularly suitable for the fermented tcm containing multiple unknown microorganism screens.
The bacterial classification of the dominant microflora of the fermentation class Chinese medicine chosen in the inventive method is rational in infrastructure, ratio suitable, can be directly used in industrialization and produce, have the advantages such as fermenting speed is fast, the cycle is short, steady quality.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises the arbitrary combination between each embodiment.
Embodiment one: present embodiment fermentation class important advantages flora selective medium comprises decocting for Chinese herbal medicine concentrated solution, glucose and agar.
Medicated Leaven, Semen Sojae Preparatum are typical fermentation class Chinese medicine in traditional Chinese medicine.Present embodiment fermentation class important advantages flora selective medium is applicable to different fermentations class Chinese medicine.
Embodiment two: the difference of present embodiment and embodiment one is: containing glucose 10 ~ 30g, agar 15 ~ 25g in the often liter of class important advantages flora selective medium that ferments.Other step and parameter identical with embodiment one.
Embodiment three: the difference of present embodiment and embodiment one is: Semen Sojae Preparatum dominant microflora selective medium is made up of soak by water concentrated solution 1000ml, the soyflour 30 ~ 50g of mulberry leaf and sweet wormwood, glucose 10 ~ 30g and agar 15 ~ 25g; The soak by water concentrated solution of mulberry leaf and sweet wormwood is decocted by the mulberry leaf of 70 ~ 100g and the sweet wormwood of 70 ~ 100g and forms.Other step and parameter identical with embodiment one.
Embodiment four: the difference of present embodiment and embodiment one is: Medicated Leaven dominant microflora selective medium is made up of soak by water concentrated solution 1000ml, the Semen Armeniacae Amarum powder 15 ~ 25g of polygonum flaccidum, sweet wormwood and Herba Xanthii grass, Semen Phaseoli powder 15 ~ 25g, glucose 10 ~ 30g and agar 15 ~ 25g; The soak by water concentrated solution of polygonum flaccidum, sweet wormwood and Herba Xanthii grass is decocted by the Herba Xanthii grass of the polygonum flaccidum of 50 ~ 100g, 50 ~ 100g sweet wormwood and 50 ~ 100g and forms.Other step and parameter identical with embodiment one.
Embodiment five: present embodiment fermentation class important advantages flora selective medium is prepared according to the following steps:
Glucose and agar are added in decocting for Chinese herbal medicine concentrated solution and shakes up, then sterilizing, namely obtain fermentation class important advantages flora selective medium.
Embodiment six: the difference of present embodiment and embodiment five is: Medicated Leaven dominant microflora selective medium preparation method:
Semen Armeniacae Amarum powder 15 ~ 25g, Semen Phaseoli powder 15 ~ 25g, glucose 10 ~ 30g and agar 15 ~ 25g are added in the soak by water concentrated solution 1000ml by polygonum flaccidum, sweet wormwood and Herba Xanthii grass and shake up, 121 DEG C again, high pressure steam sterilization 15 ~ 20min, namely obtain fermentation class important advantages flora selective medium; The soak by water concentrated solution of polygonum flaccidum, sweet wormwood and Herba Xanthii grass is decocted by the Herba Xanthii grass of the polygonum flaccidum of 50 ~ 100g, 50 ~ 100g sweet wormwood and 50 ~ 100g and forms.Other step and parameter identical with embodiment five.
Embodiment seven: the difference of present embodiment and embodiment five is: Semen Sojae Preparatum dominant microflora selective medium preparation method:
Soyflour 30 ~ 50g, glucose 10 ~ 30g and agar 15 ~ 25g are added in the soak by water concentrated solution 1000ml by mulberry leaf and sweet wormwood and shake up, then 121 DEG C, high pressure steam sterilization 15 ~ 20min, namely obtain fermentation class important advantages flora selective medium; The soak by water concentrated solution of mulberry leaf and sweet wormwood is decocted by the mulberry leaf of 70 ~ 100g and the sweet wormwood of 70 ~ 100g and forms.Other step and parameter identical with embodiment five.
Embodiment eight: present embodiment chooses fermentation class important advantages flora according to the following steps:
One, fermentation class important advantages flora selective medium is paved plate, then naturally cooling solidifies;
Two, fermentation class Chinese medicine is aseptically ground by the stroke-physiological saline solution that concentration is 0.9%, and then dilution is applied to step one and ferments on class important advantages flora selective medium, be placed in 20 DEG C ~ 40 DEG C to cultivate 2 ~ 7 days, namely obtain this fermentation class important advantages flora.
Fermentation class important advantages flora selective medium 15 ~ 20ml is poured in step one in each culture dish in present embodiment.Coating fermentation class Chinese medicine grinding diluent 50 ~ 200 μ l on step 2 fermentation class important advantages flora selective medium.
Embodiment 1
Semen Sojae Preparatum dominant microflora selective medium preparation method: soyflour 30g, glucose 20g and agar 20g are added in the soak by water concentrated solution 1000ml by mulberry leaf and sweet wormwood and shake up, 121 DEG C again, high pressure steam sterilization 15 ~ 20min, namely obtain fermentation class important advantages flora selective medium; The soak by water concentrated solution of mulberry leaf and sweet wormwood is decocted by the mulberry leaf of 70g and the sweet wormwood of 100g and forms.
Screening fermentation class important advantages flora:
One, Semen Sojae Preparatum dominant microflora selective medium is paved plate, then naturally cooling solidifies;
Two, Semen Sojae Preparatum is aseptically ground by the stroke-physiological saline solution that concentration is 0.9%, and then dilution is applied on step one Semen Sojae Preparatum dominant microflora selective medium, is placed in 25 DEG C ~ 30 DEG C and cultivates 5 days, namely obtain Semen Sojae Preparatum dominant microflora;
Semen Sojae Preparatum dominant microflora selective medium 15 ~ 20ml is poured in each culture dish in step one.
Step 2 Semen Sojae Preparatum dominant microflora selective medium applies Semen Sojae Preparatum grinding diluent 100 μ l.
Embodiment 2
Medicated Leaven dominant microflora selective medium preparation method: Semen Armeniacae Amarum powder 15g, Semen Phaseoli powder 15g, glucose 30g and agar 25g are added in the soak by water concentrated solution 1000ml by polygonum flaccidum, sweet wormwood and Herba Xanthii grass and shake up, 121 DEG C again, high pressure steam sterilization 15 ~ 20min, namely obtain fermentation class important advantages flora selective medium; The soak by water concentrated solution of polygonum flaccidum, sweet wormwood and Herba Xanthii grass is decocted by the Herba Xanthii grass of the polygonum flaccidum of 60g, 60g sweet wormwood and 60g and forms.
Screening fermentation class important advantages flora:
One, Medicated Leaven dominant microflora selective medium is down flat plate, then naturally cooling solidifies;
Two, Medicated Leaven is aseptically ground by the stroke-physiological saline solution that concentration is 0.9%, and then dilution is applied on step one Medicated Leaven dominant microflora selective medium, is placed in 25 DEG C ~ 32 DEG C and cultivates 6 days, namely obtain Medicated Leaven dominant microflora;
Medicated Leaven dominant microflora selective medium 15 ~ 20ml is poured in each culture dish in step one.
Step 2 Medicated Leaven dominant microflora selective medium applies Medicated Leaven grinding diluent 150 μ l.
Embodiment 3
With the fermentative in nutrient agar, potato dextrose agar, wort agar substratum respectively separation and Culture Semen Sojae Preparatum; Then through Semen Sojae Preparatum dominant microflora that separating for several times purifying obtains.
Embodiment 4
With the fermentative in nutrient agar, potato dextrose agar, wort agar substratum respectively separation and Culture Medicated Leaven; Then through Medicated Leaven dominant microflora that separating for several times purifying obtains.
Simultaneous test:
One, the screening of fermented bacterium is carried out with the medicine materical crude slice meeting Pharmacopoeia of the People's Republic of China version in a 2010 Semen Sojae Preparatum quality standard of method to the place of production, Anhui, the place of production, Heilungkiang, the place of production, Kunming of embodiment 1.The fermentation dominant bacteria that Anhui, Heilungkiang, ground, Kunming three adopt embodiment 1 method to filter out, (decocting liquid is admixed in clean soybean 1000g for the mulberry leaf of 70 ~ 100g and the sweet wormwood boiling of 70 ~ 100g, filtration to be inoculated into Semen Sojae Preparatum fermented substrate more respectively, wait after exhausting, steam thoroughly, take out, slightly dry in the air, put again in container, with the mulberry leaf decocted, sweet wormwood slag cover) in, then vexed, make it fermentation to yellow clothes all over time, take out, remove the dregs of a decoction, clean, then when putting vexed extremely fully fermentation, fragrance spilling in container, take out, slightly steam, dry, obtain Semen Sojae Preparatum; Fermenting process all carries out in gnotobasis, each experiment repetitive operation 5 times, and requirement that fermentation Semen Sojae Preparatum is differentiated by physics and chemistry and TLC distinguish all reaches quality standards, daidzein content all reaches 150 μ g/g, each batch products steady quality.
And the Semen Sojae Preparatum 6 batches that Anhui, Heilungkiang, ground, Kunming three adopt natural fermentating method respectively to prepare respectively, requirement that although physics and chemistry is differentiated and TLC distinguish can reach quality standards, but what daidzein content wherein only had altogether the Semen Sojae Preparatum of 3 batches reaches 150 μ g/g, and less stable, the difficult quality of product are guaranteed.
The screening of fermented bacterium is carried out with the medicine materical crude slice meeting Chinese Pharmacopoeia version in a 2010 Semen Sojae Preparatum quality standard of method to the place of production, Anhui, the place of production, Heilungkiang, the place of production, Kunming of embodiment 3.4 ~ 6 kinds, bacterium, 5 ~ 7 kinds, mould, 2 ~ 3 kinds, yeast is turned out all respectively in nutrient agar, potato dextrose agar, wort agar substratum.Then carry out respectively being separated, purifying, then combination carries out fermenting experiment in order to find out Semen Sojae Preparatum fermentation dominant bacteria mutually.Many, the consuming time length of the method experiment group, wastage of material amount is large; And the ferment effect of the best strain combination found be in Semen Sojae Preparatum daidzein content at 135 μ about g/g, still lower than by embodiment 1.
Prove that the inventive method is time saving and energy saving, fermented tcm constant product quality, be more suitable for industrialization and produce.
Two, the screening of fermented bacterium is carried out with the Medicated Leaven meeting WS3-B-35 in " Drug Standard of Ministry of Public Health of the Peoples Republic of China " of method to the place of production, Hebei, the place of production, Heilungkiang, the place of production, Kunming of embodiment 2.The fermentation dominant bacteria that the place of production, Hebei, the place of production, Heilungkiang, ground, the place of production, Kunming three adopt embodiment 2 method to filter out, then be inoculated into Medicated Leaven fermented substrate (polygonum flaccidum 500g, sweet wormwood 500g respectively, Herba Xanthii grass 500g, Semen Phaseoli 100g, Semen Armeniacae Amarum 100g, wheat bran 5000g, flour 2500g.Above seven tastes, Semen Armeniacae Amarum, Semen Phaseoli are ground into meal, mix with flour, wheat bran, separately get polygonum flaccidum, sweet wormwood, Herba Xanthii grass boiling 1 hour, filter, filtrate is condensed into cream clearly, mixes thoroughly while hot with above-mentioned medicinal powder) in, keep proper temperature and humidity, fermentation, is taken out to surface all over raw yellow-white or the mould clothing of canescence, pulverizes, drying, obtains Medicated Leaven; Fermenting process all carries out in gnotobasis, each experiment repetitive operation 5 times.Clear and definite qualitative and quantitative expedition index is not had in proper mass standard, By consulting literatures, choose proteolytic enzyme and amylase as quantitative target, the proteinase activity of each batch of Medicated Leaven product is all greater than 200U/g after testing, amylase activity is all greater than 80U/g, steady quality.
And the Medicated Leaven 6 batches that Hebei, Heilungkiang, ground, Kunming three adopt natural fermentating method respectively to prepare respectively, wherein only have altogether the proteinase activity of the Medicated Leaven of 2 batches to reach 200U/g and amylase activity is greater than 80U/g, less stable, the difficult quality of product are guaranteed.
The screening of fermented bacterium is carried out with the Medicated Leaven meeting WS3-B-35 in " Drug Standard of Ministry of Public Health of the Peoples Republic of China " of method to the place of production, Hebei, the place of production, Heilungkiang, the place of production, Kunming of embodiment 3.4 ~ 6 kinds, bacterium, 5 ~ 8 kinds, mould, 1 ~ 3 kind, yeast is turned out all respectively in nutrient agar, potato dextrose agar, wort agar substratum.Then carry out respectively being separated, purifying, then combination carries out fermenting experiment in order to find out Medicated Leaven fermentation dominant bacteria mutually.The method experiment group many, consuming time length, wastage of material amount is large, and the ferment effect of the best strain combination found is that in Medicated Leaven, proteinase activity is 150 ~ 180U/g and amylase activity is 60 ~ 65U/g, all lower than by embodiment 2.
Prove that the inventive method is time saving and energy saving, fermented tcm constant product quality, be more suitable for industrialization and produce.
Claims (6)
1. ferment class important advantages flora selective medium, it is characterized in that fermentation class important advantages flora selective medium comprises decocting for Chinese herbal medicine concentrated solution, glucose and agar.
2. fermentation class important advantages flora selective medium according to claim 1, is characterized in that in often liter of fermentation class important advantages flora selective medium containing glucose 10 ~ 30g, agar 15 ~ 25g.
3. fermentation class important advantages flora selective medium according to claim 1, is characterized in that Semen Sojae Preparatum dominant microflora selective medium is made up of soak by water concentrated solution 1000ml, the soyflour 30 ~ 50g of mulberry leaf and sweet wormwood, glucose 10 ~ 30g and agar 15 ~ 25g; The soak by water concentrated solution of mulberry leaf and sweet wormwood is decocted by the mulberry leaf of 70 ~ 100g and the sweet wormwood of 70 ~ 100g and forms.
4. fermentation class important advantages flora selective medium according to claim 1, is characterized in that Medicated Leaven dominant microflora selective medium is made up of soak by water concentrated solution 1000ml, the Semen Armeniacae Amarum powder 15 ~ 25g of polygonum flaccidum, sweet wormwood and Herba Xanthii grass, Semen Phaseoli powder 15 ~ 25g, glucose 10 ~ 30g and agar 15 ~ 25g; The soak by water concentrated solution of polygonum flaccidum, sweet wormwood and Herba Xanthii grass is decocted by the Herba Xanthii grass of the polygonum flaccidum of 50 ~ 100g, 50 ~ 100g sweet wormwood and 50 ~ 100g and forms.
5. to ferment described in claim 1 preparation method of class important advantages flora selective medium, it is characterized in that fermentation class important advantages flora selective medium is prepared according to the following steps:
Glucose and agar are added in decocting for Chinese herbal medicine concentrated solution and shakes up, then sterilizing, namely obtain fermentation class important advantages flora selective medium.
6., by the method for class important advantages flora selective medium screening fermentation class important advantages bacterium of fermenting described in claim 1, it is characterized in that carrying out according to the following steps:
One, pave plate by after the sterilizing of fermentation class important advantages flora selective medium, then naturally cooling solidifies;
Two, fermentation class Chinese medicine is aseptically ground by the stroke-physiological saline solution that concentration is 0.9%, and then dilution is applied to step one and ferments on class important advantages flora selective medium, be placed in 20 DEG C ~ 40 DEG C to cultivate 2 ~ 7 days, namely obtain this fermentation class important advantages flora.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112375698A (en) * | 2020-11-04 | 2021-02-19 | 苏州路易兴环保科技有限公司 | Chinese herbal medicine bacterial flora culture solution |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998037179A3 (en) * | 1997-02-20 | 1999-01-14 | Gist Brocades Bv | Fermentative production of valuable compounds on an industrial scale using chemically defined media |
| CN101401826A (en) * | 2008-11-11 | 2009-04-08 | 中国科学院过程工程研究所 | Method for solid-state fermentation processing process for traditional Chinese medicine |
| CN102382771A (en) * | 2011-08-25 | 2012-03-21 | 天津科技大学 | Strain produced by beta-glucosidase and method for preparing Genipin therefrom |
| CN103320474A (en) * | 2013-05-22 | 2013-09-25 | 甘肃农业大学 | Traditional Chinese medicinal bidirectional fermentation technology |
| CN104073444A (en) * | 2014-04-22 | 2014-10-01 | 杭州桐君堂生物科技有限公司 | Aspergillus oryzae TJTSW001 and application of Aspergillus oryzae in fermentation of traditional Chinese medicine Semen Sojae Praeparatum |
| CN104177168A (en) * | 2014-07-24 | 2014-12-03 | 四川光大制药有限公司 | Organic fertilizer prepared from antiviral granule medicine dregs and preparation method thereof |
| CN104922586A (en) * | 2015-01-19 | 2015-09-23 | 山东宝来利来生物工程股份有限公司 | Preparing and application of probiotics-fermented traditional Chinese medicinal preparation for preventing and treating coccidiosis in chicken |
-
2016
- 2016-01-20 CN CN201610037368.4A patent/CN105505829A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998037179A3 (en) * | 1997-02-20 | 1999-01-14 | Gist Brocades Bv | Fermentative production of valuable compounds on an industrial scale using chemically defined media |
| CN101401826A (en) * | 2008-11-11 | 2009-04-08 | 中国科学院过程工程研究所 | Method for solid-state fermentation processing process for traditional Chinese medicine |
| CN102382771A (en) * | 2011-08-25 | 2012-03-21 | 天津科技大学 | Strain produced by beta-glucosidase and method for preparing Genipin therefrom |
| CN103320474A (en) * | 2013-05-22 | 2013-09-25 | 甘肃农业大学 | Traditional Chinese medicinal bidirectional fermentation technology |
| CN104073444A (en) * | 2014-04-22 | 2014-10-01 | 杭州桐君堂生物科技有限公司 | Aspergillus oryzae TJTSW001 and application of Aspergillus oryzae in fermentation of traditional Chinese medicine Semen Sojae Praeparatum |
| CN104177168A (en) * | 2014-07-24 | 2014-12-03 | 四川光大制药有限公司 | Organic fertilizer prepared from antiviral granule medicine dregs and preparation method thereof |
| CN104922586A (en) * | 2015-01-19 | 2015-09-23 | 山东宝来利来生物工程股份有限公司 | Preparing and application of probiotics-fermented traditional Chinese medicinal preparation for preventing and treating coccidiosis in chicken |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112375698A (en) * | 2020-11-04 | 2021-02-19 | 苏州路易兴环保科技有限公司 | Chinese herbal medicine bacterial flora culture solution |
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Application publication date: 20160420 |