CN105486875A - Retinol conjugated protein detection kit - Google Patents
Retinol conjugated protein detection kit Download PDFInfo
- Publication number
- CN105486875A CN105486875A CN201610052958.4A CN201610052958A CN105486875A CN 105486875 A CN105486875 A CN 105486875A CN 201610052958 A CN201610052958 A CN 201610052958A CN 105486875 A CN105486875 A CN 105486875A
- Authority
- CN
- China
- Prior art keywords
- damping fluid
- reagent
- detection kit
- protein detection
- retinal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a retinol conjugated protein detection kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a buffer solution, the reagent R2 is the mixture of retinol conjugated protein antibody sensitized polystyrene latex particles and the buffer solution. The retinol conjugated protein detection kit has the advantages that the kit is simple and quick to detect, high in sensitivity, good in accuracy, good in anti-interference capacity and low in production cost; a lipoprotein a (retinol conjugated protein) detection method adopted by the kit is a latex enhanced immuno-turbidimetry and enables the lipoprotein a (retinol conjugated protein) detection to be more economical, convenient and quicker, and the retinol conjugated protein detection kit is applicable to automatic biochemical analyzers of most of hospitals and especially can achieve quick quantitative detection for emergency treatment.
Description
Technical field
The present invention belongs to field of medical immunology, relates to a kind of immunologic function test reagent, furtherly, the present invention relates to Retinal-binding protein detection kit detection kit.
Background technology
Flavol associated proteins (RBP) measures kit, and this product is used for Quantitative in vitro and measures RBP ELISA content in human serum, is used as auxiliary diagnosis.RBP is mainly in liver synthesis, and therefore in serum, the reduction of RBP is relevant with liver diseases with increase, and by the appearance of liver diseases and the impact of the order of severity.In hepatopathy, the serum RBP level of cirrhosis and acute hepatitis, chronic hepatitis all significantly reduces.
RBP can be used as the early diagnosis index of renal damage.During RBP ELISA RBP urinates, stability is strong, not easily decomposes, does not disturb by pH and blood pressure.When kidney proximal tubule damages, its urine discharge capacity obviously increases, therefore in urine, the increase of RBP discharge capacity can be used as the mark of kidney proximal tubule damage.When kidney filtering function reduces, in blood, because storing up, display density raises RBP.RBP concentration in blood or urine can be applied to clinical as a kind of desirable renal function index.
The mensuration of diagnostic method: RBP generally adopts RIA method, ELISA method and CLlA method, latex immunoturbidimetry etc., but adopts latex immunoturbidimetry clinically more
Serum (containing RBP)+reagent (the anti-human RBP antibody of latex) ━ ━ ━ ━ antigen antibody complex produces turbidity change, and detect under certain wavelength, the change of its turbidity becomes positive correlation with its content.
The connection of current antibody and latex uses chemical coupling method usually, and it is as R2 reagent, and very easy generation is coalescent, and reagent quality is declined along with the prolongation of resting period.Make the poor stability of reagent.
Summary of the invention
The present invention is not good according to R2 reagent stability, provides a kind of method to overcome the deficiencies in the prior art, makes it keep steady quality, provides a kind of highly sensitive, the Retinal-binding protein detection kit of good stability.
For achieving the above object, the invention provides following technical scheme:
A kind of Retinal-binding protein detection kit, described kit comprises reagent R1, reagent R2, and described reagent R1 is suitable damping fluid;
Described reagent R2 is placed in damping fluid with RBP ELISA antibody sensitized polystyrene latex particles, and the preparation process of described reagent R2 comprises:
Step (1): add ethyldimethyl amine carbodiimide (EDAC) in the polystyrene latex particles (latex) of carboxyl, obtains the latex particle activated;
Step (2): the latex particle that step (1) is activated and the mixing of RBP ELISA antibody, add glucose after question response terminates again and close unreacted radical on latex microsphere, obtain latex microsphere and the RBP ELISA antibody sensitized polystyrene latex particles of coupled antibody, be placed in damping fluid.
As preferred further: in step (2), the concentration of RBP ELISA antibody sensitized polystyrene latex particles in reagent R2 is 0.08 ~ 0.28 gram/100ml.
As preferred further: the damping fluid in described reagent R1 is at least one in PBS damping fluid, glycine buffer, borate buffer solution, acetate buffer, citrate-phosphate salt buffer, carbonate-bicarbonate damping fluid, MES (MES) damping fluid damping fluid.
As preferred further: the damping fluid in described reagent R2 is at least one in PBS damping fluid, glycine buffer, borate buffer solution, acetate buffer, citrate-phosphate salt buffer, carbonate-bicarbonate damping fluid, MES (MES) damping fluid damping fluid.
As preferred further: ethyldimethyl amine carbodiimide in step (1): the weight ratio of polystyrene latex particles is 0.5 ~ 5: 100.
As preferred further: described RBP ELISA antibody comprises polyclonal antibody or monoclonal antibody.
As preferred further: its mean grain size of polystyrene latex particles in described step (1) is between 0.01 ~ 0.2mm.
The Cleaning Principle that kit of the present invention adopts is Latex-enhanced immunoturbidimetric assay, its principle is the latex particle of RBP ELISA antibody, after RBP ELISA generation immune response, form aggregated particle, under certain wavelength, forming by measuring aggregation the turbidity produced, the content of RBP ELISA can be measured.
Advantage of the present invention and beneficial effect:
1. kit of the present invention has the advantage that detection is simple and quick, highly sensitive, accuracy is good, antijamming capability is strong and production cost is low.
2. the Retinal-binding protein detection method that the present invention adopts is latex enhancing immune turbidimetry, the method makes the detection of RBP ELISA more economical, convenient and quick, be applicable to the automatic biochemistry analyzer of most hospital, particularly can realize Quantitative detection to emergency treatment.
Embodiment
Below by embodiment, the present invention is described in further detail, but the present invention is not only confined to following examples.
The main raw material(s) that kit of the present invention relates to reagent is as follows:
1. RBP ELISA antibody: polyclonal antibody (commercially available).
2. latex: the only exemplary employing diameter of the present invention is that 80 ~ 200nm is with the polystyrene latex particles (commercially available) of carboxylic group to test.
Being formulated as follows of the main agents of the present embodiment:
Reagent R1: containing the goods damping fluid of 2.0%PEG6000 (Macrogol 6000), 95mmol/LNaCl, this reagent is colourless transparent solution.
Reagent R2: be the polystyrene latex particles of 120nm with anti-human RBP ELISA antibody sensitized particle diameter.This reagent is milky white solution.Concrete steps are as follows:
1. get 1ml (100mg/ml) latex, with 0.02M (mol/L), the MES solution (MES damping fluid) of pH5.0 washs 3 times, ultrasonic disperse;
2. then add 0.22ml 0.02M, completely, room temperature mixes 15min in the freshly prepared EDAC of MES solution (in the solution of preparation, the concentration of EDAC the is 10mg/ml) mixing of pH5.0;
3. then use 0.05M, the MES solution washing of pH5.0 2 times, the phosphate solution of 0.1M, pH7.5 washs 1 time, and ultrasonic disperse is for subsequent use;
4. get 2ml antibody (2mg/ml) solution, add 0.3ml 0.02M, the phosphate solution preparation of pH7.5, mixed at room temperature 15min,
5. then add 0.32ml10%BSA (bSA) solution, 4 DEG C of mixing 4h;
6. add 0.4ml10% glucose solution again, 4 DEG C of mixing are spent the night;
7. then use 0.015M, the glycine solution of pH7.5 washs 3 times, then adds 0.015M, and the glycine solution of pH7.5 is (containing 1%BSA, 0.2%NaN
3, 15% multitudinous sugar, 0.01%EDTA-Na
2, the glycine buffer of 0.01%trtonX-100) to latex (weight of latex that binding antibody is later) final concentration be 0.18% (mass volume ratio i.e. 0.18 gram/100ml).
The mensuration of embodiment 2 RBP ELISA
Testing tool: Hitachi 7060 type automatic analyzer.
Analytical approach: Two point end assay; Predominant wavelength: 570nm, commplementary wave length :-: sample size: 2ul (microlitre); R1:200ul; R2:50ul; The Direction of Reaction: rise; Measure temperature: 37 DEG C; After sample and R1 mix, read absorbance A 1 in the 10th second; When 3 ~ 5min, add R2, after 5min, read absorbance A 2.The difference being calculated as A2 and A1 of reaction absorbance.
Computing method: multiple spot is calibrated, and the value according to absorbance and reference serum makes dosage/response curve, and sample size can calculate on dosage/response curve according to its absorbance.
Embodiment 3 Retinal-binding protein detection kit performance evaluation
1. sensitivity for analysis assessment
Adopt 5% bovine serum albumin solution as dummy, dummy should not contain measured object.Continuous detecting 20 times on Biochemical Analyzer, calculates average and the standard deviation SD of 20 results.The detectability of twice standard deviation (+2SD) as method for reporting is added using blank average.As seen from Table 1, sensitivity is 2.68mg/L.
Table 1
2. high level is linearly assessed
By 1 part of low value serum (10mg/L) and 1 part of high level serum (100mg/L) by 9: 1,4: 1,2: 1,1: 2,1: 4,1: 9 (sample order is numbered 1 ~ No. 6), prepare 6 variable concentrations samples, each sample replication 2 times, as seen from Table 2
The highest detection scope of detection kit of the present invention can reach 91mg/L, judgment basis r2 >=0.990.
Table 2
3. precision assessment
Use the human serum sample of 2 different RBP ELISA content, measure the withinrun precision of detection kit of the present invention.Use 3 lot number kits to measure 1 human serum sample 20 times respectively, calculate detection kit of the present invention batch between relative extreme difference.Result shows, the withinrun precision of detection kit of the present invention is 5.3% and 4.19% (see table 3), and between criticizing, relative extreme difference is 2.98% (see table 4).
Table 3
Table 4
4. accuracy (recovery experiment) assessment
Select the conventional sense sample of suitable concn, 3 parts that are divided into volume identical.Add the determinand standard of different amount wherein in 2 increments bases, make the recovery sample that 2 differences add concentration, calculate the concentration of the determinand added.In another increment basis, add the solvent without measured object of same amount, make basic sample.3 replication analyses are carried out to recovery sample and basic sample, gets its average and calculate.(see table 5)
Table 5
5. interference test
Measure after adding the interfering material of different content respectively in a human serum sample.Add the measured value after interfering material and be the recovery divided by adding the measured value before interfering material, when test findings shows below the variable concentrations of cholerythrin, haemoglobin, triglyceride and Rheumatoid factors, to testing result without significantly interference (being as the criterion between 90% ~ 110% with the recovery of RBP ELISA).(see table 6).
Table 6
1. kit of the present invention has the advantage that detection is simple and quick, highly sensitive, accuracy is good, antijamming capability is strong and production cost is low.
2. the Retinal-binding protein detection method that the present invention adopts is latex enhancing immune turbidimetry, the method makes the detection of RBP ELISA more economical, convenient and quick, be applicable to the automatic biochemistry analyzer of most hospital, particularly can realize Quantitative detection to emergency treatment.
The above is only the preferred embodiment of the present invention, protection scope of the present invention be not only confined to above-described embodiment, and all technical schemes belonged under thinking of the present invention all belong to protection scope of the present invention.It should be pointed out that for those skilled in the art, some improvements and modifications without departing from the principles of the present invention, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. a Retinal-binding protein detection kit, described kit comprises reagent R1, reagent R2, and described reagent R1 is suitable damping fluid;
Described reagent R2 is placed in damping fluid with RBP ELISA antibody sensitized polystyrene latex particles, and the preparation process of described reagent R2 comprises:
Step (1): add ethyldimethyl amine carbodiimide (EDAC) in the polystyrene latex particles (latex) of carboxyl, obtains the latex particle activated;
Step (2): the latex particle that step (1) is activated and the mixing of RBP ELISA antibody, add glucose after question response terminates again and close unreacted radical on latex microsphere, obtain latex microsphere and the RBP ELISA antibody sensitized polystyrene latex particles of coupled antibody, be placed in damping fluid.
2. Retinal-binding protein detection kit according to claim 1, is characterized in that: in step (2), the concentration of RBP ELISA antibody sensitized polystyrene latex particles in reagent R2 is 0.08 ~ 0.28 gram/100ml.
3. Retinal-binding protein detection kit according to claim 1, is characterized in that: the damping fluid in described reagent R1 is at least one in PBS damping fluid, glycine buffer, borate buffer solution, acetate buffer, citrate-phosphate salt buffer, carbonate-bicarbonate damping fluid, MES (MES) damping fluid damping fluid.
4. Retinal-binding protein detection kit according to claim 1, is characterized in that: the damping fluid in described reagent R2 is at least one in PBS damping fluid, glycine buffer, borate buffer solution, acetate buffer, citrate-phosphate salt buffer, carbonate-bicarbonate damping fluid, MES (MES) damping fluid damping fluid.
5. Retinal-binding protein detection kit according to claim 1, is characterized in that: ethyldimethyl amine carbodiimide in step (1): the weight ratio of polystyrene latex particles is 0.5 ~ 5: 100.
6. Retinal-binding protein detection kit according to claim 1, is characterized in that: described RBP ELISA antibody comprises polyclonal antibody or monoclonal antibody.
7. Retinal-binding protein detection kit according to claim 1, is characterized in that: its mean grain size of polystyrene latex particles in described step (1) is between 0.01 ~ 0.2mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610052958.4A CN105486875A (en) | 2016-01-26 | 2016-01-26 | Retinol conjugated protein detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610052958.4A CN105486875A (en) | 2016-01-26 | 2016-01-26 | Retinol conjugated protein detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105486875A true CN105486875A (en) | 2016-04-13 |
Family
ID=55673992
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610052958.4A Pending CN105486875A (en) | 2016-01-26 | 2016-01-26 | Retinol conjugated protein detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105486875A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106093423A (en) * | 2016-05-31 | 2016-11-09 | 安徽伊普诺康生物技术股份有限公司 | A kind of test kit measuring retinol binding protein and preparation method thereof |
| CN106383234A (en) * | 2016-08-31 | 2017-02-08 | 上海科华生物工程股份有限公司 | Coating method for retinol-binding protein detection reagent |
| CN107478854A (en) * | 2017-08-10 | 2017-12-15 | 迈克生物股份有限公司 | A kind of LP(a) detection kit and detection method |
| CN108627652A (en) * | 2018-05-31 | 2018-10-09 | 宁波海壹生物科技有限公司 | It is a kind of to detect based on simple grain diameter and simultaneously the kit of RBP ELISA in serum and urine specimen |
| CN111190003A (en) * | 2020-03-30 | 2020-05-22 | 吉林省富生医疗器械有限公司 | Retinol binding protein detection kit and preparation method thereof |
| CN112129949A (en) * | 2020-08-18 | 2020-12-25 | 兰州百源基因技术有限公司 | Retinol binding protein detection kit, preparation method and use method thereof |
| CN116466092A (en) * | 2023-03-21 | 2023-07-21 | 浙江夸克生物科技有限公司 | Kit for quantitatively determining uroretinol binding protein |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5694266A (en) * | 1979-12-28 | 1981-07-30 | Seikagaku Kogyo Co Ltd | Anti-human retinol binding protein antibody sensitized latex for measurement of human retinol binding protein and said latex particle composition |
| CN102565419A (en) * | 2011-12-26 | 2012-07-11 | 宁波美康生物科技股份有限公司 | Myoglobin assay kit |
| CN102621332A (en) * | 2012-04-06 | 2012-08-01 | 上海蓝怡科技有限公司 | Retinol binding protein assay kit based on latex particle coating |
| CN103134934A (en) * | 2013-02-27 | 2013-06-05 | 宁波美康生物科技股份有限公司 | Kit for simultaneously detecting retinol-binding protein (RBP) in urine sample and serum sample |
| CN103384832A (en) * | 2011-02-24 | 2013-11-06 | 希尔氏宠物营养品公司 | Compositions and methods for diagnosing and treating kidney disorders in a feline |
| CN103852584A (en) * | 2014-03-28 | 2014-06-11 | 重庆中元生物技术有限公司 | Latex immune enhancement turbidimetric kit for detecting peptide C quantitatively |
-
2016
- 2016-01-26 CN CN201610052958.4A patent/CN105486875A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5694266A (en) * | 1979-12-28 | 1981-07-30 | Seikagaku Kogyo Co Ltd | Anti-human retinol binding protein antibody sensitized latex for measurement of human retinol binding protein and said latex particle composition |
| CN103384832A (en) * | 2011-02-24 | 2013-11-06 | 希尔氏宠物营养品公司 | Compositions and methods for diagnosing and treating kidney disorders in a feline |
| CN102565419A (en) * | 2011-12-26 | 2012-07-11 | 宁波美康生物科技股份有限公司 | Myoglobin assay kit |
| CN102621332A (en) * | 2012-04-06 | 2012-08-01 | 上海蓝怡科技有限公司 | Retinol binding protein assay kit based on latex particle coating |
| CN103134934A (en) * | 2013-02-27 | 2013-06-05 | 宁波美康生物科技股份有限公司 | Kit for simultaneously detecting retinol-binding protein (RBP) in urine sample and serum sample |
| CN103852584A (en) * | 2014-03-28 | 2014-06-11 | 重庆中元生物技术有限公司 | Latex immune enhancement turbidimetric kit for detecting peptide C quantitatively |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106093423A (en) * | 2016-05-31 | 2016-11-09 | 安徽伊普诺康生物技术股份有限公司 | A kind of test kit measuring retinol binding protein and preparation method thereof |
| CN106383234A (en) * | 2016-08-31 | 2017-02-08 | 上海科华生物工程股份有限公司 | Coating method for retinol-binding protein detection reagent |
| CN106383234B (en) * | 2016-08-31 | 2017-11-24 | 上海科华生物工程股份有限公司 | The method for coating of Retinal-binding protein detection reagent |
| CN107478854A (en) * | 2017-08-10 | 2017-12-15 | 迈克生物股份有限公司 | A kind of LP(a) detection kit and detection method |
| CN108627652A (en) * | 2018-05-31 | 2018-10-09 | 宁波海壹生物科技有限公司 | It is a kind of to detect based on simple grain diameter and simultaneously the kit of RBP ELISA in serum and urine specimen |
| CN111190003A (en) * | 2020-03-30 | 2020-05-22 | 吉林省富生医疗器械有限公司 | Retinol binding protein detection kit and preparation method thereof |
| CN112129949A (en) * | 2020-08-18 | 2020-12-25 | 兰州百源基因技术有限公司 | Retinol binding protein detection kit, preparation method and use method thereof |
| CN116466092A (en) * | 2023-03-21 | 2023-07-21 | 浙江夸克生物科技有限公司 | Kit for quantitatively determining uroretinol binding protein |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105486875A (en) | Retinol conjugated protein detection kit | |
| CN102353770B (en) | Detection kit for cystine protease inhibitor C | |
| CN101769932B (en) | Full-range C-reactive protein detection kit | |
| CN103777023B (en) | Kit for measuring adiponectin by nano latex enhanced immune turbidimetry | |
| CN102590497B (en) | Cysteine protease inhibitor C test kit | |
| CN101819207B (en) | Kit for detecting alpha 1-microglobulin by nano microsphere immunonephelometry | |
| CN102507918B (en) | Kit for determining anti-cyclic citrullinated peptide (Anti-CCP) antibody | |
| CN102628865B (en) | Latex enhanced immunoturbidimetry kit for detection of myoglobin content | |
| CN102621332B (en) | Retinol binding protein assay kit based on latex particle coating | |
| CN105675891A (en) | Kit for testing lipoprotein a(Lp(a)) | |
| CN105372434A (en) | Detection kit for human serum amyloid A protein | |
| CN102175871A (en) | Liquid double-reagent kit for measuring free light chains in serum or urine by double-latex method | |
| CN103645323B (en) | A kind of cystatin C detection kit and preparation method thereof | |
| CN102565419B (en) | Myoglobin assay kit | |
| CN101377492A (en) | Bladder chalone C determining reagent kit | |
| CN102944679A (en) | Kit for performing retinol binding protein detection by using latex turbidimetry | |
| CN106353507A (en) | Kit for detecting serum amyloid protein and application thereof | |
| CN104215770A (en) | Two-particle-based retinol binding protein detection kit | |
| CN102539784B (en) | Method for detecting cardiac troponin I and application thereof | |
| CN102636653A (en) | Compounded latex particle-enveloped cystatin C detection kit | |
| CN101915849A (en) | Method for measuring glycosylated hemoglobin content | |
| WO2010064435A1 (en) | Method for measuring cystatin c in human body fluid | |
| CN106932589A (en) | Determine kit of human serum RBP ELISA content and preparation method thereof | |
| CN105606822A (en) | Beta 2-microglobulin detection kit | |
| CN106370860A (en) | Kit and test paper strip for serum immunoglobulin E colloidal gold chromatography quantitative detection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160413 |
|
| RJ01 | Rejection of invention patent application after publication |