CN105478177A - Droplet generation device and method used for digital PCR - Google Patents
Droplet generation device and method used for digital PCR Download PDFInfo
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- CN105478177A CN105478177A CN201410477281.XA CN201410477281A CN105478177A CN 105478177 A CN105478177 A CN 105478177A CN 201410477281 A CN201410477281 A CN 201410477281A CN 105478177 A CN105478177 A CN 105478177A
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- drop
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- digital pcr
- droplet generation
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Abstract
The invention relates to a droplet generation device and a droplet generation method used for digital PCR. The invention discloses the droplet generation method used for digital PCR. According to the invention, a droplet generation process comprises the two steps: original droplet generation, and droplet break up. In the droplet break up step, one droplet is broken up into two under mechanical force. With the method, a large amount of droplets can be generated fast and stably. The volumes of the droplets are uniform, such that a good result repeatability is ensured, and variance is small. Droplet generation time consumption is reduced, needed experiment time is saved, and timeliness is improved.
Description
Technical field
The present invention relates to a kind of drop formation device and method for digital pcr.
Background technology
It is stable that to generate a large amount of microlayer model fast extremely important for drop digital pcr.The volume of drop is homogeneous, and ensure that result is reproducible, variance is little.The quantity of drop, determines the template DNA quantitative range can surveyed in the range of linearity.Drop formation is quick, shortens drop formation consuming time, can save experiment required time, improves real-time.And this technology does not also occur in the market.
Summary of the invention
For the deficiencies in the prior art, the object of this invention is to provide and a kind ofly can stablize the method generating a large amount of microlayer model fast.
For achieving the above object, present invention employs following technical scheme:
For a drop formation method for digital pcr, drop formation comprises two steps: one is that original drop generates, and two is drop breakup, and described drop breakup makes a drop under mechanical force, and division becomes two.
As the further optimization of such scheme, described drop breakup comprises multilevel block sizes, and namely original drop is by one-level division generation two sub-drops of one-level; The sub-drop of each one-level generates two sub-drops of secondary by second-order splitting; The sub-drop of each secondary becomes two three grades of sub-drops by three fraction schizogenesis, and drop formation number increases and exponentially level increase with division progression, and the volume of drop reduces fast simultaneously.
Present invention also offers the generating apparatus of above-mentioned drop, it comprises original tube, the end of this original tube splits into the diode of two same specifications, and each diode splits into the triode of two same specifications, number exponentially level increase along with the increase of division progression of N level pipe.
As the further optimization of such scheme, the specification of described N level pipe is less than the specification of N-1 level pipe.
Beneficial effect main manifestations of the present invention is: adopt drop formation apparatus and method of the present invention, and stablize and generate a large amount of microlayer model fast, the volume of drop is homogeneous, and ensure that result is reproducible, variance is little; Drop formation is quick, shortens drop formation consuming time, saves experiment required time, improves real-time.
Detailed description of the invention
Below in conjunction with preferred embodiment, the present invention is more specifically described.
Drop formation comprises two steps: original drop generates, drop breakup.Drop breakup is that division becomes the technology of two by changing channels designs, making a drop under mechanical force.Drop breakup can design becomes multilevel hierarchy, thus realizes generating the exponential increase of number of drops object.Namely original drop generates two sub-drops of one-level by primary structure; The sub-drop of each one-level generates two sub-drops of secondary by secondary structure; The sub-drop of each secondary can generate two three grades of sub-drops by tertiary structure.Thus realizing the exponentially level increase with the increase of microfluidic system structure complexity of drop formation number, the volume of drop reduces fast simultaneously.The volume of drop is less, and its stability is better.
In conjunction with preferred embodiment, the present invention is explained in detail above, but the invention is not restricted to above-mentioned embodiment, in the ken that those of ordinary skill in the art possess, can also make a variety of changes under the prerequisite not departing from present inventive concept.
Do not depart from the spirit and scope of the present invention and can make other changes many and remodeling.Should be appreciated that and the invention is not restricted to specific embodiment, scope of the present invention is defined by the following claims.
Claims (4)
1. for a drop formation method for digital pcr, it is characterized in that, drop formation comprises two steps: one is that original drop generates, and two is drop breakup,
Described drop breakup makes a drop under mechanical force, and division becomes two.
2. the drop formation method for digital pcr according to claim 1, it is characterized in that, described drop breakup comprises multilevel block sizes, and namely original drop is by one-level division generation two sub-drops of one-level; The sub-drop of each one-level generates two sub-drops of secondary by second-order splitting; The sub-drop of each secondary becomes two three grades of sub-drops by three fraction schizogenesis, and drop formation number increases and exponentially level increase with division progression, and the volume of drop reduces fast simultaneously.
3. the generating apparatus of the drop described in claim 1 or 2, it is characterized in that, it comprises original tube, and the end of this original tube splits into the diode of two same specifications, each diode splits into the triode of two same specifications, number exponentially level increase along with the increase of division progression of N level pipe.
4. the generating apparatus of drop according to claim 3, is characterized in that, the specification of described N level pipe is less than the specification of N-1 level pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410477281.XA CN105478177A (en) | 2014-09-18 | 2014-09-18 | Droplet generation device and method used for digital PCR |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410477281.XA CN105478177A (en) | 2014-09-18 | 2014-09-18 | Droplet generation device and method used for digital PCR |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105478177A true CN105478177A (en) | 2016-04-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201410477281.XA Pending CN105478177A (en) | 2014-09-18 | 2014-09-18 | Droplet generation device and method used for digital PCR |
Country Status (1)
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| CN (1) | CN105478177A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2030790A1 (en) * | 2007-08-31 | 2009-03-04 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO | Droplet break-up device |
| CN101533005A (en) * | 2009-04-14 | 2009-09-16 | 北京大学 | Microflow distribution device, manufacturing method and application thereof |
| CN102187216A (en) * | 2008-08-15 | 2011-09-14 | 华盛顿大学 | Method and apparatus for the discretization and manipulation of sample volumes |
| WO2012109138A1 (en) * | 2011-02-07 | 2012-08-16 | President And Fellows Of Harvard College | Systems and methods for splitting droplets |
| CN103285947A (en) * | 2013-05-27 | 2013-09-11 | 苏州扬清芯片科技有限公司 | Droplet micro-fluidic chip and operation method thereof |
| CN104004652A (en) * | 2014-06-16 | 2014-08-27 | 东南大学 | Method for manufacturing digital PCR liquid drop array chip based on single-jet ink-jet printing |
-
2014
- 2014-09-18 CN CN201410477281.XA patent/CN105478177A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2030790A1 (en) * | 2007-08-31 | 2009-03-04 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO | Droplet break-up device |
| CN102187216A (en) * | 2008-08-15 | 2011-09-14 | 华盛顿大学 | Method and apparatus for the discretization and manipulation of sample volumes |
| CN101533005A (en) * | 2009-04-14 | 2009-09-16 | 北京大学 | Microflow distribution device, manufacturing method and application thereof |
| WO2012109138A1 (en) * | 2011-02-07 | 2012-08-16 | President And Fellows Of Harvard College | Systems and methods for splitting droplets |
| CN103285947A (en) * | 2013-05-27 | 2013-09-11 | 苏州扬清芯片科技有限公司 | Droplet micro-fluidic chip and operation method thereof |
| CN104004652A (en) * | 2014-06-16 | 2014-08-27 | 东南大学 | Method for manufacturing digital PCR liquid drop array chip based on single-jet ink-jet printing |
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Application publication date: 20160413 |
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| WD01 | Invention patent application deemed withdrawn after publication |