CN105168085B - Mescenchymal stem cell derives liquid and preparation method thereof and the application in cosmetics - Google Patents
Mescenchymal stem cell derives liquid and preparation method thereof and the application in cosmetics Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
- A61K8/988—Honey; Royal jelly, Propolis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
The invention discloses a kind of mescenchymal stem cells to derive liquid and preparation method thereof and the application in cosmetics, and the preparation method which derives liquid includes the following steps：(1) mescenchymal stem cell of placenta adipose tissue-derived is cultivated using mescenchymal stem cell culture medium, collects cell culture fluid；(2) 13 propylene glycol, Sodium Hyaluronate and royal jelly are added into cell culture fluid obtained by step (1) and derive liquid to get mescenchymal stem cell.Gained mescenchymal stem cell derives liquid and is used for cosmetics, and moistening effect is good；Microgroove can be significantly reduced, improve skin smoothness；Improve skin elasticity；Improve heat flush, the situation of decortication and inflammation, reduces the generation of skin allergy.
Description
Technical field
The present invention relates to a kind of skin care item and its preparation method and application, more particularly to a kind of mescenchymal stem cell derives liquid
And preparation method thereof and the application in cosmetics.
Background technology
Since the 21th century, with country continuous development, social is constantly progressive, the improvement of people's living standards and
The pursuit beautiful to itself, people especially women payes attention to daily life cosmetology further, and makeup currently on the market
The good and bad jumbled together for product product, very different, and consumer is difficult to differentiate.
Many fine chemistry industry skin care item contain lead mercury, ethyl alcohol, colorant and mineral oil etc. to the harmful substance of human skin,
Consumer while using these skin care item, unconsciously causes autologous skin certain damage daily, serious to go back
It can cause dermatitis and infection problems.And the cosmetics that existing market is sold, it is by plant component extraction or fining mostly
Work manufacture craft is process, and the macromolecular substances in product are not easy to be absorbed by the skin, and causes the added burden of skin.In addition
Due to technical bottleneck and production capacity, the cosmetic product of current stage does not all have bioactivity substantially, cannot be fundamentally
The living environment and metabolism, the skin lesion for caused by extraneous factor for improving epidermal cell do not have repair, especially
It is not suitable for skin sensitivity or pregnant woman uses.
Invention content
In order to overcome the deficiencies of existing technologies, the present invention provides a kind of mescenchymal stem cells to derive liquid and preparation method thereof
With the application in cosmetics.It is good that the mescenchymal stem cell derives liquid moistening effect；Microgroove can be significantly reduced, improve skin smooth
Degree；Improve skin elasticity；Improve heat flush, the situation of decortication and inflammation, reduces the generation of skin allergy；Preparation method letter
It is single, large-scale promotion can be carried out and form industrialization production processing
A kind of mescenchymal stem cell derives the preparation method of liquid, includes the following steps：
(1) mescenchymal stem cell of placenta adipose tissue-derived is cultivated using mescenchymal stem cell culture medium, is received
Collect cell culture fluid；
(2) 1-3 propylene glycol, Sodium Hyaluronate and royal jelly are added into cell culture fluid obtained by step (1) to get filling
The stem cell-derived liquid of matter.
A kind of mescenchymal stem cell derives the preparation method of liquid, further preferably into cell culture fluid obtained by step (1) plus
Enter growth factor, glutathione, trehalose and chitosan oligosaccharide.
A kind of mescenchymal stem cell derives the preparation method of liquid, in the mescenchymal stem cell derives liquid：1-3 propylene glycol
A concentration of 50-100g/L；A concentration of 5-10mg/L of Sodium Hyaluronate；A concentration of 3~6mg/L of royal jelly.
A kind of mescenchymal stem cell derives the preparation method of liquid, wherein the growth factor includes：Stem cell factor,
Antagonistic melanin growth factor and vascular endothelial growth factor, in mescenchymal stem cell derives liquid：Stem cell factor
A concentration of the 1 of a concentration of 0.5~2mg/L, a concentration of 3~5mg/L of antagonistic melanin growth factor, vascular endothelial growth factor
~3mg/L；A concentration of 2~8mg/L of glutathione；A concentration of 4~6mg/L of trehalose；A concentration of 2~8mg/ of chitosan oligosaccharide
L。
A kind of mescenchymal stem cell derives the preparation method of liquid, in mescenchymal stem cell derives liquid：Preferably make 1-3 third
A concentration of 5mg/L, the stem cell factor of a concentration of 100g/L of glycol, a concentration of 8mg/L of Sodium Hyaluronate, royal jelly
A concentration of 1.5mg/L, a concentration of 4mg/L of antagonistic melanin growth factor, vascular endothelial growth factor a concentration of 2mg/
L, a concentration of 4mg/L of a concentration of 5mg/L of glutathione, a concentration of 5mg/L of trehalose, chitosan oligosaccharide.Chitosan oligosaccharide is called low
Chitosan is by chitosan through obtained a kind of degree of polymerization oligosaccharide product between 2~20 of degrading, molecular weight≤3200Da,
It is water-soluble low molecular weight product preferable, function is big, bioactivity is high.
A kind of mescenchymal stem cell derives the preparation method of liquid, wherein the mescenchymal stem cell culture medium passes through business
It is suitble to the culture medium of adipose tissue-derived mescenchymal stem cell culture obtained by channel, such as：German promocell companies
, name of product be growth of mesenchymal stem cells culture medium.The culture medium is dry thin for the human mesenchymal of adipose tissue-derived
Born of the same parents' design, it can support the amplification of human mesenchymal stem cells, but not the cell quilt observed in standard medium occur
The phenomenon that leading to aging ahead of time and differentiation, which does not have any animal derived materials, in addition to mankind's blood of people source
Pure albumen.
A kind of mescenchymal stem cell derives the preparation method of liquid, wherein the mesenchyma of the placenta adipose tissue-derived is dry thin
Born of the same parents obtain in the following manner：(1) placenta tissue is rinsed, it is preferable to use the sulfate buffer containing erythromycin rinses tire
Disk tissue；(2) after placenta tissue being processed into fritter, preferably 1~2mm3Fritter, then dermal fat level is laid in downward
In culture dish, epidermis is removed after neutral proteinase II, 18-24h is added, is obtained between the placenta adipose tissue-derived pre-processed
Mesenchymal stem cells.Placenta tissue, which is cut into small pieces, can obtain best survival rate.
A kind of mescenchymal stem cell derives the preparation method of liquid, wherein the mesenchyma to placenta adipose tissue-derived is dry
It includes original cuiture that cell, which carries out culture, further includes preferably secondary culture to obtain more cell culture fluids, described primary
Conventional method in the prior art may be used in culture and secondary culture, such as：Mould is added in mescenchymal stem cell culture medium
Element and streptomysin, it is 100U/ml to make its concentration, in 37 DEG C, 3-5%CO2Under conditions of cultivated, per 2-5 days replacement one
Secondary Mesenchymal stem cell nutrient solution；It passes on when cell growth to 80% Fusion Strain, is digested with 0.25% pancreatin when passage
3-5 minutes, make cell detachment bottle wall, supernatant is abandoned into cell suspending liquid centrifugation, cell dilutes 2-3 times and carries out secondary culture, 37
DEG C, 3-5%CO2Under conditions of when carrying out secondary culture, preferred culture 14 days per a generation.
A kind of mescenchymal stem cell derives the preparation method of liquid, and it is preferred that cell culture fluid is wherein collected described in step (1)
Collect the cell culture fluid for being passaged to 3-7 generations.When cell growth to 3~7 generation, generate to the advantageous activated protein of skin
Molecule reaches peak-peak, but when passage number was more than 7 generation will produce the metabolite unfavorable to skin histology, therefore obtains
It is most suitable to take and be passaged to the cell culture fluid in 3~7 generations.
A kind of mescenchymal stem cell derives the preparation method of liquid, wherein the placenta is preferably derived from people.
A kind of mescenchymal stem cell derivative liquid obtained by above-mentioned preparation method.
A kind of above-mentioned mescenchymal stem cell derives application of the liquid in cosmetics.
Compared with prior art, the present invention has the advantages that：
1, mescenchymal stem cell derivative liquid energy of the present invention, which enough plays, promotes skin epithelial cell division growth, radioresistance antioxygen
The skin effect for the effects that changing and removing free radical；Long-acting moistening effect is good；Microgroove can be significantly reduced, improve skin smoothness, made
16% is reduced with the average most deep wrinkle depth of test group after 8 weeks, average wrinkle depth reduces 9%, and average wrinkle total quantity subtracts
Few 10%；Improve skin elasticity, 21% is increased using the mean skin elasticity number of test group after 28 days；Improve heat flush shape
Condition；And mescenchymal stem cell of the present invention derives liquid and does not occur adverse reaction, has good development in the market in cosmetics and skincare product
Space；
2, mescenchymal stem cell derivative liquid of the present invention has broken away from traditional plant component extraction and fine chemistry industry production technology,
It is obtained containing close human skin using the method for cell engineering, by cultivating the mescenchymal stem cell of Human plactnta adipose tissue-derived
Beneficial activity protein molecular Mesenchymal stem cell nutrient solution, using gained cell culture fluid as entire product mesenchymal stem cells
The basic components of derivative liquid, and chemical industry additive is not added in mescenchymal stem cell derivative liquid, such as：Ethyl alcohol, lead mercury, coloring
The sensitization toxin such as agent, mineral oil utmostly ensure that the safety that product persistently uses；
3,1-3 propylene glycol and royal jelly is added in mescenchymal stem cell derives liquid in the present invention, can improve skin histology
Oxidation resistance, repair skin ageing phenomenon that illumination causes, improve dermal layer of the skin histocyte and fibroblastic
Division growth power；The present invention adds dry growth factor, antagonistic melanin growth factor, blood in mescenchymal stem cell derives liquid
Endothelial tube growth factor, these types of growth factor, which has, to be promoted skin heavy metal ion toxin to exclude, strengthens skin barrier defence
Function, to the oxidation resistant effect of Green Tea Extract；Active factors therein include stem cell factor, antagonistic melanin growth because
Son and vascular endothelial growth factor have better effect for the whitening and moisturizing reparation of skin.The present invention is also especially added with sea
Algae sugar can promote to generate, reinforce the defence of the external boundary's environment of skin keratin confluent monolayer cells in collagen and elastomer in skin
Function；The various formula components that mescenchymal stem cell of the present invention derives in liquid cooperate with mutually, are achieved its best skin and protect
Support effect；
4, the preparation method of mescenchymal stem cell derivative liquid of the present invention is simple, can carry out large-scale promotion and form industrialization
Production and processing.
Description of the drawings
Fig. 1 is to test front and back test group and control group volunteer when carrying out improving skin elasticity measure of merit in embodiment 4
The datagram of skin elasticity value；
Fig. 2 is when carrying out improving the test of heat flush situation in embodiment 4, and volunteer uses the skin before and after product of the present invention
Skin picture.
Specific implementation mode
Below in conjunction with the accompanying drawings, the specific implementation mode of the present invention is described in detail, it is to be understood that the guarantor of the present invention
Shield range is not restricted by specific implementation.
The mescenchymal stem cell culture medium that following embodiment uses is German promocell companies, entitled mesenchyma
The product of stem cell growth culture medium；The mescenchymal stem cell of Human plactnta adipose tissue-derived in following embodiment be by with
What under type obtained：It is cut into 1mm after rinsing human placenta3Fritter tiles the dermal fat level of the tissue downward
In culture dish, after DispaseII (neutral proteinase) is added for 24 hours, epidermis is removed, the placenta adipose tissue pre-processed is obtained
The mescenchymal stem cell in source；Original cuiture in following embodiment is in 37 DEG C, 3%CO2Under conditions of cultivated, every 2
It replaces a Mesenchymal stem cell nutrient solution, is passed on when cell growth to 80% Fusion Strain；Biography in following embodiment
It is commissioned to train to support and is：Digestion 3 minutes is carried out to primary cultured cell with 0.25% trypsase when passage, makes cell detachment bottle wall,
Bovine serum albumin is added and terminates reaction, after cell is suspended and centrifuges and abandons supernatant, cell dilutes 2 times and carries out secondary cultures, 37
DEG C, 3%CO2Under conditions of carry out secondary culture, it is each be commissioned to train foster 14 days after descend a generation again.
Embodiment 1
A kind of mescenchymal stem cell derives the preparation method of liquid, includes the following steps：
(1) mescenchymal stem cell culture medium is used to carry out primary training to the mescenchymal stem cell of Human plactnta adipose tissue-derived
It was passaged to for the 7th generation after supporting, collects the 7th generation cell culture fluid；
(2) 1-3 propylene glycol, Sodium Hyaluronate, royal jelly, stem cell life are added into cell culture fluid obtained by step (1)
The long factor, antagonistic melanin growth factor, vascular endothelial growth factor, glutathione, trehalose and chitosan oligosaccharide make 1-3 the third two
A concentration of 100g/L of alcohol, a concentration of 8mg/L of Sodium Hyaluronate, a concentration of 5mg/L of royal jelly, stem cell factor
A concentration of 1.5mg/L, a concentration of 4mg/L of antagonistic melanin growth factor, vascular endothelial growth factor a concentration of 2mg/L,
A concentration of 5mg/L of glutathione, a concentration of 5mg/L of trehalose, a concentration of 4mg/L of chitosan oligosaccharide are dry thin to get mesenchyma
Born of the same parents derive liquid.
Embodiment 2
(1) mescenchymal stem cell culture medium is used to carry out primary training to the mescenchymal stem cell of Human plactnta adipose tissue-derived
It was passaged to for the 3rd generation after supporting, collects the 3rd generation cell culture fluid；
(2) be added into cell culture fluid obtained by step (1) 1-3 propylene glycol, Sodium Hyaluronate, royal jelly, growth factor,
Glutathione, trehalose and chitosan oligosaccharide make a concentration of 50g/L, a concentration of 10mg/L of Sodium Hyaluronate, bee of 1-3 propylene glycol
A concentration of 3mg/ of a concentration of 3mg/L of royal jelly, a concentration of 2mg/L of stem cell factor, antagonistic melanin growth factor
L, a concentration of 6mg/L, the shell of a concentration of 2mg/L of a concentration of 3mg/L, glutathione of vascular endothelial growth factor, trehalose
A concentration of 2mg/L of oligosaccharides derives liquid to get mescenchymal stem cell.
Embodiment 3
(1) mescenchymal stem cell culture medium is used to carry out primary training to the mescenchymal stem cell of Human plactnta adipose tissue-derived
It was passaged to for the 7th generation after supporting, collects the 7th generation cell culture fluid；
(2) 1-3 propylene glycol, Sodium Hyaluronate, royal jelly are added into cell culture fluid obtained by step (1), makes 1-3 the third two
A concentration of 80g/L of alcohol, a concentration of 5mg/L of Sodium Hyaluronate, royal jelly a concentration of 6mg/L to get mescenchymal stem cell
Derivative liquid.
4 contrast test of embodiment
1, moistening effect is tested
100 18-50 Sui healths, the male of dry skin, women mix volunteer, are classified as test group at random
And control group, every group of each 50 people make the volunteer of test group and control group in 24 DEG C of room temperature, in the environment of humidity 50% before experiment
Keep 12h after, using corneometer CM825 test probes measure skin moisture content (instrument can detect epidermis with
Moisture near lower 100 μm), the average moisture content for measuring test group is 38.9%, and the average moisture content of control group is
37.6%, the two is not significantly different；After starting experiment, test group uses the mescenchymal stem cell for obtaining embodiment 1
Derivative liquid 10% mescenchymal stem cell for being diluted with water and being obtained after 10 times that applies some make up derives liquid, and control group uses 10% transparent
Matter acid sodium solution；Application method is once in the morning and once at night to be applied to volunteer's inner forearm the same area (25mm × 25mm),
Continuous use 28 days makes test group and control group after last time is using test specimen and reference substance on the day of testing the 28th day
Volunteer keep 12h in 24 DEG C of room temperature, the environment of humidity 50%, use corneometer CM825 test probes measure
The average moisture content of skin moisture content, test group is 70.3%, and the average moisture content of control group is 51.4%, it can be seen that real
The mescenchymal stem cell derivative long-acting moistening effect of liquid applied in example 1 is notable.After 24 hours, the test group still to 90% or more
Volunteer has moistening effect.
2, anti-wrinkle effect is tested
The male, women of 40 50-60 Sui healths mix volunteer, are classified as test group and control group at random,
Every group of each 20 people；Test group, which uses to apply some make up the mescenchymal stem cell derivative liquid that embodiment 1 obtains, is diluted with water 10
10% mescenchymal stem cell obtained after times derives liquid, and control group uses Blank gel；Application method is：Twice daily,
It takes and is applied to circumference of eyes in right amount, gently loop to fully absorbing, be used continuously 8 weeks, distinguish using before and after sample and reference substance
Silica gel reverse mould digital image analysis is done to eye periphery wrinkle, using rear experimental group number according to as follows：Average most deep wrinkle depth minus
Few 16%, average wrinkle depth reduces 9%, and average wrinkle total quantity reduces 10%；It is as follows using rear control group data：Averagely most
Deep wrinkle depth increases 2%, and average wrinkle depth reduces 3%, and average wrinkle total quantity reduces 2%.
3, improve skin elasticity measure of merit
The male, women of 30 18-50 Sui healths mix volunteer, are classified as test group and control group at random,
Every group of 15 people, test group, which uses to apply some make up the mescenchymal stem cell derivative liquid that embodiment 1 obtains, is diluted with water 10 times
10% mescenchymal stem cell obtained afterwards derives liquid, and control group uses Blank gel；Application method is：Morning and evening each one daily
It is secondary to be applied to volunteer's inner forearm the same area (25mm × 25mm), it is used continuously 28 days, using skinanalysis apparatus (Derma-
Expert MC760) skin elasticity measurement is carried out to test group before and after experiment and control group volunteer, as shown in Figure 1, test group
Data are as follows：5%, 9%, 21% has been increased separately using mean skin elasticity number after 1 week, 2 weeks and 4 weeks；Control group data are such as
Under：2%, 4%, 4.5% has been increased separately using mean skin elasticity number after 1 week, 2 weeks and 4 weeks.
4, improve the test of heat flush situation
22 18-50 Sui healths, heat flush, the male of easy allergy, women mix volunteer, and being used in face will
The mescenchymal stem cell that embodiment 1 obtains derives liquid 20% mescenchymal stem cell for being diluted with water and being obtained after 5 times that applies some make up and spreads out
The case where raw liquid, service life are 30 days, 85% or more user's heat flush is improved, and comparison diagram is see Fig. 2.
Disclosed above is only specific embodiments of the present invention, and still, the present invention is not limited to this, any this field
What technical staff can think variation should all fall into protection scope of the present invention.
Claims (10)
1. a kind of mescenchymal stem cell derives the preparation method of liquid, which is characterized in that include the following steps：
(1) mescenchymal stem cell of placenta adipose tissue-derived is cultivated using mescenchymal stem cell culture medium, is collected thin
Born of the same parents' culture solution；
(2) it is dry to get mesenchyma that 1-3 propylene glycol, Sodium Hyaluronate and royal jelly are added into cell culture fluid obtained by step (1)
Cell-derived liquid；
Wherein, in the mescenchymal stem cell of gained derives liquid：A concentration of 50-100g/L of 1-3 propylene glycol；Sodium Hyaluronate
A concentration of 5-10mg/L；A concentration of 3~6mg/L of royal jelly.
2. mescenchymal stem cell according to claim 1 derives the preparation method of liquid, which is characterized in that also to step (1)
Growth factor, glutathione, trehalose and chitosan oligosaccharide are added in gained cell culture fluid.
3. mescenchymal stem cell according to claim 2 derives the preparation method of liquid, which is characterized in that the growth factor
Including：Stem cell factor, antagonistic melanin growth factor and vascular endothelial growth factor derive liquid in mescenchymal stem cell
In：It is a concentration of 0.5~2mg/L of stem cell factor, a concentration of 3~5mg/L of antagonistic melanin growth factor, intravascular
A concentration of 1~3mg/L of skin growth factor；A concentration of 2~8mg/L of glutathione；A concentration of 4~6mg/L of trehalose；Shell
A concentration of 2~8mg/L of oligosaccharides.
4. mescenchymal stem cell according to claim 3 derives the preparation method of liquid, which is characterized in that dry thin in mesenchyma
Born of the same parents derive in liquid：A concentration of 100g/L of 1-3 propylene glycol；A concentration of 8mg/L of Sodium Hyaluronate, a concentration of 5mg/ of royal jelly
L, a concentration of 1.5mg/L of stem cell factor, antagonistic melanin growth factor a concentration of 4mg/L, vascular endothelial growth
A concentration of 2mg/L of the factor, a concentration of 5mg/L of glutathione, a concentration of 5mg/L of trehalose, chitosan oligosaccharide it is a concentration of
4mg/L。
5. the mescenchymal stem cell according to one of claim 1-4 derives the preparation method of liquid, which is characterized in that the tire
The mescenchymal stem cell of disk adipose tissue-derived obtains in the following manner：(1) placenta tissue is rinsed, it is preferable to use containing
The sulfate buffer of erythromycin rinses placenta tissue；(2) after placenta tissue being processed into fritter, preferably 1~2mm3Fritter,
Dermal fat level is laid in culture dish downward again, epidermis is removed after neutral proteinase II, 18-24h is added, obtains pre- place
The mescenchymal stem cell for the placenta adipose tissue-derived managed.
6. the mescenchymal stem cell according to one of claim 1-4 derives the preparation method of liquid, which is characterized in that described right
It includes original cuiture that the mescenchymal stem cell of placenta adipose tissue-derived, which carries out culture,.
7. the mescenchymal stem cell according to one of claim 1-4 derives the preparation method of liquid, which is characterized in that described right
It further includes secondary culture that the mescenchymal stem cell of placenta adipose tissue-derived, which carries out culture,.
8. the mescenchymal stem cell according to one of claim 1-4 derives the preparation method of liquid, which is characterized in that step
(1) cell culture fluid is collected described in collects the cell culture fluid for being passaged to 3-7 generations.
9. the mescenchymal stem cell that a kind of preparation method by described in one of claim 1-8 obtains derives liquid.
10. the mescenchymal stem cell described in a kind of claim 9 derives application of the liquid in cosmetics.
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| CN106074356A (en) * | 2016-08-04 | 2016-11-09 | 深圳市合康生物科技股份有限公司 | A kind of preparation method and applications of living cells essence |
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| CN107397710B (en) * | 2017-07-31 | 2021-02-23 | 广州润虹医药科技股份有限公司 | Preparation method and application of skin stem cell active element |
| CN110151673A (en) * | 2019-05-09 | 2019-08-23 | 嘉文丽（福建）化妆品有限公司 | A kind of pure liquid composition for improving skin containing stem cell medium |
| CN110917111A (en) * | 2019-12-30 | 2020-03-27 | 上海掘谜生物科技有限公司 | Preparation method of mesenchymal stem cell derived liquid and application of mesenchymal stem cell derived liquid in cosmetics |
| CN112725270B (en) * | 2021-03-29 | 2021-07-30 | 北京益华生物科技有限公司 | Human-derived bone marrow mesenchymal stem cell induction culture medium and induction method |
| CN113384517B (en) * | 2021-07-16 | 2021-12-31 | 上海汉氏方舟生物科技有限公司 | Preparation method of stem cell cytokine and application of stem cell cytokine in preparation of medicines or cosmetics |
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| CN103550117A (en) * | 2013-10-18 | 2014-02-05 | 上海润生生物技术有限公司 | Beauty and skin care product capable of promoting skin repairation and regeneration as well as preparation method and application thereof |
| WO2015028900A1 (en) * | 2013-08-29 | 2015-03-05 | Stempeutics Research Pvt. Ltd. | Stromal cells derived conditioned medium, method of obtaining said conditioned medium compositions, formulations and applications thereof |
| CN104762260A (en) * | 2015-04-23 | 2015-07-08 | 广州赛莱拉干细胞科技股份有限公司 | Preparation method and application of adipose-derived mesenchymal stem cells and preparation thereof |
| CN104840371A (en) * | 2014-02-17 | 2015-08-19 | 国玺干细胞应用技术股份有限公司 | mesenchymal stem cell extract and use thereof |
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2015
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102395353A (en) * | 2009-03-20 | 2012-03-28 | 生命医学股份公司 | Composition for improving skin conditions using fetal mesenchymal stem cells from amniotic fluid |
| WO2015028900A1 (en) * | 2013-08-29 | 2015-03-05 | Stempeutics Research Pvt. Ltd. | Stromal cells derived conditioned medium, method of obtaining said conditioned medium compositions, formulations and applications thereof |
| CN103550117A (en) * | 2013-10-18 | 2014-02-05 | 上海润生生物技术有限公司 | Beauty and skin care product capable of promoting skin repairation and regeneration as well as preparation method and application thereof |
| CN104840371A (en) * | 2014-02-17 | 2015-08-19 | 国玺干细胞应用技术股份有限公司 | mesenchymal stem cell extract and use thereof |
| CN104762260A (en) * | 2015-04-23 | 2015-07-08 | 广州赛莱拉干细胞科技股份有限公司 | Preparation method and application of adipose-derived mesenchymal stem cells and preparation thereof |
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