CN105154517A - Method for detecting disinfection effects of liquid soap - Google Patents
Method for detecting disinfection effects of liquid soap Download PDFInfo
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- CN105154517A CN105154517A CN201510406477.4A CN201510406477A CN105154517A CN 105154517 A CN105154517 A CN 105154517A CN 201510406477 A CN201510406477 A CN 201510406477A CN 105154517 A CN105154517 A CN 105154517A
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- hand
- liquid soap
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- hands
- safety equipment
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
The invention discloses a method for detecting disinfection effects of liquid soap. The method includes steps of S1, confirming that the surfaces of detected hands are sterile; S2, confirming that the surfaces of the detected hands are contaminated by detected bacteria; S3, confirming the quantities of the bacteria contaminated on the surfaces of the detected hands; S4, verifying the disinfection effects of the liquid soap; S5, repeatedly carrying out the steps S1, S2, S3 and S4 by 6 times in bio-safety cabinets in order to guarantee accurate inspection results, respectively recording the inspection results and fetching mean values. The sterilization efficiency meets an equation of sterilization efficiency=(the mean values of the inspection results of the step S3-the mean values of the inspection results of the step S4)/the mean values of the inspection results of the step S3*100%. The method has the advantages that gaps in the field are filled, accordingly, detection bases for proving the effectiveness of products can be provided for manufacturers, and selection bases for the quality of the products can be provided for consumers.
Description
Technical field
The present invention relates to sterilisation effect detection method technical field, be specifically related to a kind of Liquid soap sterilisation effect detection method.
Background technology
The main function of Liquid soap plays the effect of clean skin care, some specific composition can play the effect of sterilization, sterilization, such as intestinal bacteria, streptococcus aureus, streptococcus etc., but do not have at present because the sterilisation effect of Liquid soap series products detects in prior art
countrywith the detection method of industry, human consumer lacks corresponding criterion and according to selecting sterilization equipment, it is effective to prove the product of oneself that manufacturer lacks corresponding examination criteria too.
Therefore prior art haves much room for improvement and develops.
Summary of the invention
The technical problem to be solved in the present invention is to provide one conveniently Liquid soap sterilisation effect detection method.
Technical solution of the present invention is: Liquid soap sterilisation effect detection method, comprises the following steps,
S1: confirm tested hand surface sterile, comprise the following steps:
S11: testing crew is first washed one's hands 3 times 10 minutes with suds in Biohazard Safety Equipment Medicine and Surgery handwashing disinfection mode totally, hand is steeped 5 ~ 10 minutes afterwards with 72% alcohol, dry, smear the whole hand palm of the hand and the back of the hand by stroke-physiological saline solution, S12: detect total number of bacterial colony with baste;
S2: the tested mushroom of tested hand surface contamination, concrete operation step is as follows:
S21: both hands are immersed in 3 minutes rear ventings in bacterium liquid in Biohazard Safety Equipment and dry; Described bacterium liquid is that in the standard models such as mould, yeast, Salmonellas, streptococcus aureus, colon bacillus, any one cultivates preparation;
S3: confirm tested hand surface microbiological contamination quantity, concrete operation step is as follows:
S31: smear the palm of the hand and each 2 square centimeters of the back of the hand, S32 by stroke-physiological saline solution in Biohazard Safety Equipment: baste detects total number of bacterial colony;
S4: checking Liquid soap sterilisation effect, concrete operation step is as follows:
Wash one's hands with the Liquid soap that need detect in Biohazard Safety Equipment, wash one's hands the scheduled time, aseptic water washing is clean, and ventilation is dried, and smears the palm of the hand and each 2 square centimeters of the back of the hand by stroke-physiological saline solution, and baste detects total number of bacterial colony;
S5: result judges and calculates germ-killing efficiency:
Result judges:
If S12 detects that bacterium colony is for there being bacterium, re-starts experiment in order;
If S32 does not detect bacterium colony, re-start experiment in order;
Germ-killing efficiency=(S3 assay value-S4 assay value)/S3 assay value * 100%.
For ensureing that assay is accurate, in Biohazard Safety Equipment, S1, S2, S3, S4 are repeated 6 times suddenly, records tests result respectively is also averaged; Its germ-killing efficiency=(S3 assay mean value-S4 assay mean value)/S3 assay mean value * 100%.
Beneficial effect of the present invention: the sterilisation effect due to Liquid soap series products detects not to be had at present
countrywith the detection method of industry, human consumer lacks corresponding criterion and foundation selects sterilization equipment, it is effective to prove the product of oneself that manufacturer lacks corresponding examination criteria too, the generation of present method can make up the blank in this field, for the producer provides the validity detected according to proving product, for human consumer provides the selection gist of quality product.
Embodiment
Embodiment:
Liquid soap sterilisation effect detection method, comprises the following steps,
S1: confirm that tested hand is aseptic, comprise the following steps:
S11: testing crew is first washed one's hands 3 times 10 minutes with suds in Biohazard Safety Equipment Medicine and Surgery handwashing disinfection mode totally, hand is steeped 5 ~ 10 minutes afterwards with 72% alcohol, dry, smear whole hand front and the back side by stroke-physiological saline solution, S12: detect total number of bacterial colony with baste;
S2: confirm the tested mushroom of tested hand surface contamination, concrete operation step is as follows:
S21: both hands are immersed in 3 minutes rear ventings in bacterium liquid in Biohazard Safety Equipment and dry; Described bacterium liquid is that in the standard models such as mould, yeast, Salmonellas, streptococcus aureus, colon bacillus, any one cultivates preparation;
S3: confirm tested hand surface microbiological contamination quantity, concrete operation step is as follows:
S31: smear the palm of the hand and each 2 square centimeters of the back of the hand, S32 by stroke-physiological saline solution in Biohazard Safety Equipment: baste detects total number of bacterial colony;
S4: checking Liquid soap sterilisation effect, concrete operation step is as follows:
Wash one's hands with the Liquid soap that need detect in Biohazard Safety Equipment, wash one's hands the scheduled time, aseptic water washing is clean, and ventilation is dried, and smears whole hand front and the back side by stroke-physiological saline solution, and baste detects total number of bacterial colony;
S5: result judges and calculates germ-killing efficiency:
Result judges:
If S12 detects that bacterium colony is for there being bacterium, re-starts experiment in order;
If S32 does not detect bacterium colony, re-start experiment in order;
Germ-killing efficiency=(S3 assay value-S4 assay value)/S3 assay value * 100%.
For ensureing that assay is accurate, in Biohazard Safety Equipment, S1, S2, S3, S4 are repeated 6 times suddenly, records tests result respectively is also averaged; Its germ-killing efficiency=(S3 assay mean value-S4 assay mean value)/S3 assay mean value * 100%.
In the description of this specification sheets, specific features, structure, material or feature that the description of term " embodiment " etc. means to describe in conjunction with this embodiment or example are contained at least one embodiment of the present invention or example.In this manual, identical embodiment or example are not necessarily referred to the schematic representation of above-mentioned term.And the specific features of description, structure, material or feature can combine in an appropriate manner in any one or more embodiment or example.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (2)
1. Liquid soap sterilisation effect detection method, is characterized in that: comprise the following steps,
S1: confirm tested hand surface sterile, comprise the following steps:
S11: testing crew is first washed one's hands 3 times 10 minutes with suds in Biohazard Safety Equipment Medicine and Surgery handwashing disinfection mode totally, hand is steeped 5 ~ 10 minutes afterwards with 72% alcohol, dry, smear the whole hand palm of the hand and the back of the hand by stroke-physiological saline solution, S12: detect total number of bacterial colony with baste;
S2: the tested mushroom of tested hand surface contamination, concrete operation step is as follows:
S21: both hands are immersed in 3 minutes rear ventings in bacterium liquid in Biohazard Safety Equipment and dry; Described bacterium liquid is that in the standard models such as mould, yeast, Salmonellas, streptococcus aureus, colon bacillus, any one cultivates preparation;
S3: confirm tested hand surface microbiological contamination quantity, concrete operation step is as follows:
S31: smear the palm of the hand and each 2 square centimeters of the back of the hand, S32 by stroke-physiological saline solution in Biohazard Safety Equipment: baste detects total number of bacterial colony;
S4: checking Liquid soap sterilisation effect, concrete operation step is as follows:
Wash one's hands with the Liquid soap that need detect in Biohazard Safety Equipment, wash one's hands the scheduled time, aseptic water washing is clean, and ventilation is dried, and smears the palm of the hand and each 2 square centimeters of the back of the hand by stroke-physiological saline solution, and baste detects total number of bacterial colony;
S5: result judges and calculates germ-killing efficiency:
Result judges:
If S12 detects that bacterium colony is for there being bacterium, re-starts experiment in order;
If S32 does not detect bacterium colony, re-start experiment in order;
Germ-killing efficiency=(S3 assay value-S4 assay value)/S3 assay value * 100%.
2. Liquid soap sterilisation effect detection method according to claim 1, is characterized in that:
For ensureing that assay is accurate, in Biohazard Safety Equipment, S1, S2, S3, S4 are repeated 6 times suddenly, records tests result respectively is also averaged; Its germ-killing efficiency=(S3 assay mean value-S4 assay mean value)/S3 assay mean value * 100%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510406477.4A CN105154517A (en) | 2015-07-12 | 2015-07-12 | Method for detecting disinfection effects of liquid soap |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510406477.4A CN105154517A (en) | 2015-07-12 | 2015-07-12 | Method for detecting disinfection effects of liquid soap |
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| CN105154517A true CN105154517A (en) | 2015-12-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510406477.4A Pending CN105154517A (en) | 2015-07-12 | 2015-07-12 | Method for detecting disinfection effects of liquid soap |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112494161A (en) * | 2020-11-09 | 2021-03-16 | 佛山市第一人民医院（中山大学附属佛山医院） | Surgical hand disinfection effect detection system and detection method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5900067A (en) * | 1995-05-16 | 1999-05-04 | Jones; C. Kerry | Handwashing technique analysis |
| CN1596877A (en) * | 2003-09-16 | 2005-03-23 | 上海拜耳科生物科技有限公司 | Antiseptic hand cleaning liquid with out water cleaning and its preparation method |
| CN101176700A (en) * | 2007-10-29 | 2008-05-14 | 广东庆发药业有限公司 | Antimicrobial disinfection hand cleanser and preparation method thereof |
| CN102321728A (en) * | 2011-06-10 | 2012-01-18 | 洛阳惠中兽药有限公司 | Evaluation method for disinfection effect of disinfectant |
-
2015
- 2015-07-12 CN CN201510406477.4A patent/CN105154517A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5900067A (en) * | 1995-05-16 | 1999-05-04 | Jones; C. Kerry | Handwashing technique analysis |
| CN1596877A (en) * | 2003-09-16 | 2005-03-23 | 上海拜耳科生物科技有限公司 | Antiseptic hand cleaning liquid with out water cleaning and its preparation method |
| CN101176700A (en) * | 2007-10-29 | 2008-05-14 | 广东庆发药业有限公司 | Antimicrobial disinfection hand cleanser and preparation method thereof |
| CN102321728A (en) * | 2011-06-10 | 2012-01-18 | 洛阳惠中兽药有限公司 | Evaluation method for disinfection effect of disinfectant |
Non-Patent Citations (3)
| Title |
|---|
| 吴玲等: "白猫消毒洗手液杀菌效果的实验观察", 《齐齐哈尔医学院学报》 * |
| 戴岚等: "免冲洗洗手液的杀菌效果与毒性观察", 《中国消毒学杂志》 * |
| 沈伟等: "一次性使用卫生用品卫生标准", 《中华人民共和国国家标准》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112494161A (en) * | 2020-11-09 | 2021-03-16 | 佛山市第一人民医院（中山大学附属佛山医院） | Surgical hand disinfection effect detection system and detection method |
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Application publication date: 20151216 |
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