CN105153478A - Method for preparing composite film by using crustacean - Google Patents
Method for preparing composite film by using crustacean Download PDFInfo
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- CN105153478A CN105153478A CN201510399381.XA CN201510399381A CN105153478A CN 105153478 A CN105153478 A CN 105153478A CN 201510399381 A CN201510399381 A CN 201510399381A CN 105153478 A CN105153478 A CN 105153478A
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Abstract
The present invention discloses a method for preparing a composite film by using crustacean. The technical process comprises that crab shell is adopted as a raw material to prepare chitosan, nanometer cellulose crystal, an antioxidant and a plasticizer (enzymatic fish skin collagen peptide) are assistedly added to prepare a film-forming solution, finally the blended film-forming solution is poured onto a polyvinyl chloride crystal plate, and scraping, drying and film removing are performed to prepare the composite antibacterial and anti-oxidation film. According to the present invention, the crab shell is adopted as the main raw material so as to provide the effective approach for the high-value utilization of the offcuts; and the prepared composite antibacterial and anti-oxidation film has significant biological activity, and has good application potential in the food packaging.
Description
Technical field
The invention belongs to food processing field, relate to the preparation method of packing film, particularly relate to a kind of method adopting Crustacean to prepare composite membrane.
Background technology
Edible film is with polysaccharide, protein, lipid etc. for raw material, the film with porous network structure formed by differing molecular interphase interaction.Film forming material can be divided into by functional performance: host (polyose, protein), hydrophobizing agent (wax, lipid acid etc.) and softening agent (polyvalent alcohol, lipid etc.).Edible film, by the migration of prevention gas, aqueous vapor and solute etc., is avoided or is delayed the change of the aspect such as food generation local flavor and matter structure, extend Food Shelf-life, or reduce packing cost.
At present, along with the raising of development and the people's living standard gradually of science and technology, the surrogate as plastics film has been not content with in requirement for edible film, more wish that it has more functional performance, as in edible film production process, add the materials such as antiseptic-germicide, antioxidant, pigment, flavour substances, nutrition-fortifying agent, spice.Therefore, composite membrane becomes new development trend.
In recent years, along with the development of China's fish processing industry, the output of tankage is increasing, and reasonable development aquatic products processing tankage become marine industry urgent need to solve the problem.
Summary of the invention
There are some researches show, in aquatic products processing tankage, fish-skin is rich in collagen protein, and shrimp shell is rich in chitin, and fish is first-class is rich in DHA and EPA, is the high quality raw material of protective foods, has larger potentiality to be exploited.Crab class is processed and in use procedure, crab shell Chang Zuowei refuse is abandoned, and causes larger pressure to environment.There are some researches show, be rich in chitin in crab shell, reasonable development will produce larger economic benefit.Based on this, according to the present Research of edible film and crab shell, with crab shell for raw material, by series of processes process, prepare a kind of composite antibacterial, oxidation-resistant film.
The object of the present invention is to provide a kind of method adopting Crustacean to prepare composite membrane.Its product prepared is as follows containing, for example lower component proportions by weight: chitosan prepared by crab shell: nano cellulose crystal: antioxidant (tea-polyphenol): fishskin gelatin fluidizer=100:3 ~ 5:2 ~ 3:25 ~ 30.
For achieving the above object, technical scheme of the present invention is:
The present invention is a kind of method adopting Crustacean to prepare composite membrane, it prepares the chitosan in crab shell by certain technique, then in chitosan solution, add nano cellulose crystal, antioxidant and softening agent (enzymolysis fish skin collagen peptide), obtain composite antibacterial, oxidation-resistant film after drying and moulding, concrete steps are as follows:
(1) preparation of chitosan in crab shell:in crab shell powder, 4 ~ 6%HCl solution is added, in soaking at room temperature 5 ~ 6h decalcification, drying according to solid-liquid ratio 1g:15 ~ 25mL; Then in powder after drying, add 4 ~ 6%NaOH by solid-liquid ratio 1g:15 ~ 25mL, soak 3 ~ 4h deproteination matter, drying in 95 DEG C; The hydrogen peroxide finally adding 2 ~ 3% by solid-liquid ratio 1g:15 ~ 25mL in powder soaks 5 ~ 6h decolouring in 45 DEG C, and residuum is 60 ~ 65 DEG C of oven dry in an oven, pulverize, obtain chitin powder; In chitin powder, add 45 ~ 55%NaOH by solid-liquid ratio 1g:10 ~ 20mL, 95 DEG C are soaked 12 ~ 15h, and filter, distilled water wash 3 times, obtains crab shell chitosan.
(2) preparation of fishskin gelatin fluidizer:in the fish-skin shredded, add the NaOH solution of 1mol/L according to solid-liquid ratio 1g:20 ~ 25mL, stir 4 ~ 6h, be washed to neutrality; Then join in 0.3 ~ 0.5%HCl according to solid-liquid ratio 1g:15 ~ 20mL, soaking at room temperature 2 ~ 3 days, every 12h changes water once; The fish-skin homogenate finally will processed, joins in distilled water according to solid-liquid ratio 1g:10 ~ 12mL, in 65 ~ 70 DEG C of dynamic extraction 10 ~ 12h, then in the centrifugal 15 ~ 20min of 9000g, gets supernatant liquor, and concentrated, freeze-drying, obtains fishskin gelatin.Getting fishskin gelatin joins in Glycine-NaOH damping fluid (0.05mol/L, pH9.5) by solid-liquid ratio 1g:20 ~ 25mL, adds Sumizyme MP (enzyme activity>=2 × 10 according to 1 ~ 2% of fishskin gelatin weight
5), after 40 DEG C of hydrolysis 2 ~ 3h, being cooled to room temperature, is then that 3kDa and 10kDa ultra-filtration membrane carries out ultrafiltration with retaining component, collects molecular weight and is greater than 3kDa and is less than 10kDa component, obtain gelatin fluidizer.
(3) preparation of composite antibacterial, oxidation-resistant film film-forming soln: according to solid-liquid ratio 1.5g:80 ~ 100mL, chitosan prepared by step (1) is joined in distilled water, stir 80 ~ 90min in 40 ~ 50 DEG C to dissolve completely to chitosan, then join in chitosan solution according to 25 ~ 30% fishskin gelatin fluidizers prepared by step (2) of chitosan weight, 2 ~ 3% antioxidants of chitosan weight, 3 ~ 5% nano cellulose crystals of chitosan weight, ultrasonic 10 ~ 15min under stirring at normal temperature, continue stirring 60 ~ 90min de-bubbled in room temperature after ultrasonic, make film-forming soln.
(4) preparation of composite antibacterial, oxidation-resistant film:be poured on equably by the film-forming soln modulated on polyvinyl chloride crystal slab and carry out striking film forming, in 40 ~ 50% times dryings of 40 DEG C of relative humidity after 1 ~ 1.5 day, namely demoulding obtains crab shell composite antibacterial, oxidation-resistant film.
As improvement, crab shell described in step (1), its source for Portunus trituberculatus Miers (
portunustrituberculatus).
As improvement, fish-skin described in step (2), its source for green fin black scraper Puffer (
thamnaconusseptentrionalis).
As improvement, described in step (3), antioxidant is tea-polyphenol.
Compared with prior art, the invention has the advantages that:
1, the present invention is with crab shell for raw material, can improve the added value of crab class processing, turn waste into wealth, and reduces aquatic products processing tankage to the pollution of environment.
2, the present invention prepare composite antibacterial, oxidation-resistant film fluidizer be black scraper Puffer fish-skin enzymolysis collagen peptide, safe and reliable.
3, composite antibacterial, the oxidation-resistant film that prepared by the present invention contain tea-polyphenol, can suppress by UV-induced food peroxidatic reaction of lipid, extend the shelf-lives of food.Meanwhile, the gelatin fluidizer in composite membrane has significant bacteriostatic activity.Therefore, this composite membrane, can the growth of anti-lipid peroxidation and pathogenic microorganism as packaging material for food, extends the shelf-lives of food, improves food quality.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Adopt Crustacean to prepare a method for composite membrane, preparation technology's flow process is as follows: crab shell-decalcification, removing protein-chitosan-film-forming soln preparation-composite antibacterial, oxidation-resistant film.
Embodiment:
(1) preparation of crab shell chitosan:according to solid-liquid ratio 1g:25mL to Portunus trituberculatus Miers (
portunustrituberculatus) add 5%HCl solution, in soaking at room temperature 6h decalcification, drying in crab shell powder; Then in powder after drying, add 4%NaOH by solid-liquid ratio 1g:25mL, soak 4h deproteination matter, drying in 95 DEG C; The hydrogen peroxide finally adding 2 ~ 3% by solid-liquid ratio 1g:15mL in powder soaks 5h decolouring in 45 DEG C, and residuum is 65 DEG C of oven dry in an oven, pulverize, obtain chitin powder; In chitin powder, add 45%NaOH by solid-liquid ratio 1g:20mL, 95 DEG C are soaked 12h, and filter, distilled water wash 3 times, obtains crab shell chitosan.
(2) preparation of fishskin gelatin fluidizer:according to solid-liquid ratio 1g:25mL to rub green fin black scraper Puffer (
thamnaconusseptentrionalis) add the NaOH solution of 1mol/L in fish-skin, stir 6h, be washed to neutrality; Then join in 0.5%HCl according to solid-liquid ratio 1g:15mL, soaking at room temperature 3 days, every 12h changes water once; The fish-skin homogenate finally will processed, joins in distilled water according to solid-liquid ratio 1g:12mL, in 70 DEG C of dynamic extraction 12h, then in the centrifugal 15min of 9000g, gets supernatant liquor, and concentrated, freeze-drying, obtains fishskin gelatin.Getting fishskin gelatin joins in Glycine-NaOH damping fluid (0.05mol/L, pH9.5) by solid-liquid ratio 1g:25mL, adds Sumizyme MP (enzyme activity>=2 × 10 according to 1.5% of fishskin gelatin weight
5), after 40 DEG C of hydrolysis 2h, being cooled to room temperature, is then that 3kDa and 10kDa ultra-filtration membrane carries out ultrafiltration with retaining component, collects molecular weight and is greater than 3kDa and is less than 10kDa component, obtain gelatin fluidizer.
(3) preparation of composite antibacterial, oxidation-resistant film film-forming soln:according to solid-liquid ratio 1.5g:80mL, chitosan prepared by step (1) is joined in distilled water, stir 80min in 45 DEG C to dissolve completely to chitosan, then join in chitosan solution according to 25% fishskin gelatin fluidizer prepared by step (2) of chitosan weight, 2% antioxidant (tea-polyphenol) of chitosan weight, 3% nano cellulose crystal of chitosan weight, ultrasonic 15min under stirring at normal temperature, continue to stir 70min de-bubbled in room temperature after ultrasonic, make film-forming soln.
(4) preparation of composite antibacterial, oxidation-resistant film:be poured on equably by the film-forming soln modulated on polyvinyl chloride crystal slab and carry out striking film forming, in the 45% time drying of 40 DEG C of relative humidity after 1.5 days, namely demoulding obtains crab shell composite antibacterial film.The method of the composite antibacterial film prepared by the present invention has antibacterial and anti-oxidant activity, and achieving unexpected food preservation effect can promote the use of at food packaging applications.
Finally, still need it is noted that what enumerate above is only a specific embodiment of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be had.All distortion that those of ordinary skill in the art can directly derive from content disclosed by the invention or associate, all should think protection scope of the present invention.
Claims (5)
1. adopt Crustacean to prepare a method for composite membrane, it is characterized in that: it comprises the following steps:
(1) preparation of chitosan in crab shell:in crab shell powder, 4 ~ 6%HCl solution is added, in soaking at room temperature 5 ~ 6h decalcification, drying according to solid-liquid ratio 1g:15 ~ 25mL; Then in powder after drying, add 4 ~ 6%NaOH by solid-liquid ratio 1g:15 ~ 25mL, soak 3 ~ 4h deproteination matter, drying in 95 DEG C; The hydrogen peroxide finally adding 2 ~ 3% by solid-liquid ratio 1g:15 ~ 25mL in powder soaks 5 ~ 6h decolouring in 45 DEG C, and residuum is 60 ~ 65 DEG C of oven dry in an oven, pulverize, obtain chitin powder; In chitin powder, add 45 ~ 55%NaOH by solid-liquid ratio 1g:10 ~ 20mL, 95 DEG C are soaked 12 ~ 15h, and filter, distilled water wash 3 times, obtains crab shell chitosan;
(2) preparation of fishskin gelatin fluidizer:in the fish-skin shredded, add the NaOH solution of 1mol/L according to solid-liquid ratio 1g:20 ~ 25mL, stir 4 ~ 6h, be washed to neutrality; Then join in 0.3 ~ 0.5%HCl according to solid-liquid ratio 1g:15 ~ 20mL, soaking at room temperature 2 ~ 3 days, every 12h changes water once; The fish-skin homogenate finally will processed, joins in distilled water according to solid-liquid ratio 1g:10 ~ 12mL, in 65 ~ 70 DEG C of dynamic extraction 10 ~ 12h, then in the centrifugal 15 ~ 20min of 9000g, gets supernatant liquor, and concentrated, freeze-drying, obtains fishskin gelatin.
2. getting fishskin gelatin joins in Glycine-NaOH damping fluid (0.05mol/L, pH9.5) by solid-liquid ratio 1g:20 ~ 25mL, adds Sumizyme MP (enzyme activity>=2 × 10 according to 1 ~ 2% of fishskin gelatin weight
5), after 40 DEG C of hydrolysis 2 ~ 3h, being cooled to room temperature, is then that 3kDa and 10kDa ultra-filtration membrane carries out ultrafiltration with retaining component, collects molecular weight and is greater than 3kDa and is less than 10kDa component, obtain gelatin fluidizer;
(3) preparation of composite antibacterial, oxidation-resistant film film-forming soln: according to solid-liquid ratio 1.5g:80 ~ 100mL, chitosan prepared by step (1) is joined in distilled water, stir 80 ~ 90min in 40 ~ 50 DEG C to dissolve completely to chitosan, then join in chitosan solution according to 25 ~ 30% fishskin gelatin fluidizers prepared by step (2) of chitosan weight, 2 ~ 3% antioxidants of chitosan weight, 3 ~ 5% nano cellulose crystals of chitosan weight, ultrasonic 10 ~ 15min under stirring at normal temperature, continue stirring 60 ~ 90min de-bubbled in room temperature after ultrasonic, make film-forming soln;
(4) preparation of composite antibacterial, oxidation-resistant film:be poured on equably by the film-forming soln modulated on polyvinyl chloride crystal slab and carry out striking film forming, in 40 ~ 50% times dryings of 40 DEG C of relative humidity after 1 ~ 1.5 day, namely demoulding obtains crab shell composite antibacterial, oxidation-resistant film.
3. a kind of method adopting Crustacean to prepare composite membrane according to claim 1, is characterized in that: crab shell described in step (1), and its source is Portunus trituberculatus Miers.
4. a kind of method adopting Crustacean to prepare composite membrane according to claim 1, is characterized in that: fish-skin described in step (2), and its source is green fin black scraper Puffer.
5. a kind of method adopting Crustacean to prepare composite membrane according to claim 1, is characterized in that: described in step (3), antioxidant is tea-polyphenol.
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Cited By (11)
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| CN105951441A (en) * | 2016-06-16 | 2016-09-21 | 东莞市联洲知识产权运营管理有限公司 | Chitosan fiber non-woven fabric with periostracum cicadae as raw material and preparation method of chitosan fiber non-woven fabric |
| CN107683951A (en) * | 2017-07-19 | 2018-02-13 | 安徽王家坝生态农业有限公司 | A kind of processing method of flavor fish jelly |
| CN108126231A (en) * | 2017-12-01 | 2018-06-08 | 高产明 | A kind of preparation method of the antibacterial hospital gauze of high intensity |
| CN108690251A (en) * | 2018-06-12 | 2018-10-23 | 芜湖蓝天工程塑胶有限公司 | Food fresh-keeping film and preparation method thereof |
| CN109369961A (en) * | 2018-10-16 | 2019-02-22 | 河南工程学院 | A kind of nano-cellulose based film material and preparation method thereof of polypeptide enhancing |
| CN110746520A (en) * | 2019-12-03 | 2020-02-04 | 扬州日兴生物科技股份有限公司 | Novel efficient preparation method for chitosan production |
| CN113412831A (en) * | 2021-06-18 | 2021-09-21 | 浙江理工大学 | Storage method of insect specimen |
| CN113797388A (en) * | 2021-09-24 | 2021-12-17 | 长春工业大学 | Preparation method of chitin artificial skin membrane |
| CN115259936A (en) * | 2022-06-27 | 2022-11-01 | 大连益丰农生物科技有限公司 | Gelatin selenium peptide-chitin composite plant protection spraying film agent and preparation method thereof |
| CN116035163A (en) * | 2023-01-09 | 2023-05-02 | 厦门绿进食品有限公司 | Preparation method for keeping color of meat product prefabricated dish |
| CN117582557A (en) * | 2024-01-19 | 2024-02-23 | 四川恒普科技有限公司 | Demineralized bone fiber and preparation method thereof |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105951441A (en) * | 2016-06-16 | 2016-09-21 | 东莞市联洲知识产权运营管理有限公司 | Chitosan fiber non-woven fabric with periostracum cicadae as raw material and preparation method of chitosan fiber non-woven fabric |
| CN107683951A (en) * | 2017-07-19 | 2018-02-13 | 安徽王家坝生态农业有限公司 | A kind of processing method of flavor fish jelly |
| CN108126231A (en) * | 2017-12-01 | 2018-06-08 | 高产明 | A kind of preparation method of the antibacterial hospital gauze of high intensity |
| CN108690251A (en) * | 2018-06-12 | 2018-10-23 | 芜湖蓝天工程塑胶有限公司 | Food fresh-keeping film and preparation method thereof |
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| CN109369961A (en) * | 2018-10-16 | 2019-02-22 | 河南工程学院 | A kind of nano-cellulose based film material and preparation method thereof of polypeptide enhancing |
| CN110746520A (en) * | 2019-12-03 | 2020-02-04 | 扬州日兴生物科技股份有限公司 | Novel efficient preparation method for chitosan production |
| CN113412831A (en) * | 2021-06-18 | 2021-09-21 | 浙江理工大学 | Storage method of insect specimen |
| CN113412831B (en) * | 2021-06-18 | 2022-04-08 | 浙江理工大学 | Storage method of insect specimen |
| CN113797388A (en) * | 2021-09-24 | 2021-12-17 | 长春工业大学 | Preparation method of chitin artificial skin membrane |
| CN115259936A (en) * | 2022-06-27 | 2022-11-01 | 大连益丰农生物科技有限公司 | Gelatin selenium peptide-chitin composite plant protection spraying film agent and preparation method thereof |
| CN115259936B (en) * | 2022-06-27 | 2024-05-17 | 大连益丰农生物科技有限公司 | Gelatin selenopeptide-chitin composite plant protection spray film agent and preparation method thereof |
| CN116035163A (en) * | 2023-01-09 | 2023-05-02 | 厦门绿进食品有限公司 | Preparation method for keeping color of meat product prefabricated dish |
| CN117582557A (en) * | 2024-01-19 | 2024-02-23 | 四川恒普科技有限公司 | Demineralized bone fiber and preparation method thereof |
| CN117582557B (en) * | 2024-01-19 | 2024-03-19 | 四川恒普科技有限公司 | Demineralized bone fiber and preparation method thereof |
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