CN105085719B - Polymerization tank - Google Patents

Polymerization tank Download PDF

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Publication number
CN105085719B
CN105085719B CN201510570733.3A CN201510570733A CN105085719B CN 105085719 B CN105085719 B CN 105085719B CN 201510570733 A CN201510570733 A CN 201510570733A CN 105085719 B CN105085719 B CN 105085719B
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temperature level
temperature
sample
low
ultraviolet lamp
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CN105085719A (en
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吴彤
邓汝芳
徐信兰
贾永霞
夏快飞
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South China Botanical Garden of CAS
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South China Botanical Garden of CAS
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Abstract

The invention provides a polymerization tank. The polymerization tank comprises an insulating shell body which is provided with a sample chamber and an insulating cover body covering the insulating shell body, wherein a sample rack is arranged in the sample chamber of the insulating shell body; an ultraviolet lamp chamber which is arranged corresponding to the sample rack is embedded inside the insulating cover body; and a refrigeration system is arranged in the insulating shell body, and a refrigeration evaporator in the refrigeration system is arranged on the side wall of the sample chamber. According to the polymerization tank, the temperature can be accurately controlled, the cooling speed is high, strong ultraviolet ray can be provided to facilitate polymerization of biological samples, and biological samples manufactured by utilizing the polymerization tank have stable quality.

Description

Polymerization case
Technical field
The invention mainly relates to biological sample preparing technical field, more particularly to polymerization box technology field.
Background technology
Transmission electron microscope can see the fine structure less than 0.2um that cannot be seen clearly under an optical microscope, it It is with electron beam as " light source ", using electromagnetic lens to carrying the Ultrastructural electron beam in sample and putting through sample Greatly, then the Ultrastructural enlarged drawing of sample is shown on fluorescent screen, it is and certain mechanical device, electronics and Gao Zhen One of comprehensive precise electronic optical instrument of modernization that empty technology combines and constitutes.Conventional transmission electron microscope skill Art is limited only to observe the ultra microstructure of plant cell, it is impossible to which some particular matters are positioned in cell.Closely A little Colloidal Gold Immunoelectron Microscopy Techniques for growing up in year are the milestones in electron microscopy history, and it is under cryogenic progressively with having Machine solvent replaces the water in sample, and the polymerizing curable sample under ultraviolet cryogenic conditions, in conjunction with collaurum after ultra-thin section The antibody labeling antigen of particle, and transmission electron microscope observation is used, so as to obtain positioning of the antigen in subcellular structure.This During the utilization of technology can solve the problems, such as biological sample preparation process, antigen active keeps, and also can effectively reduce organic solvent opposite The injury of thing sample, especially reduces the injury to antigen.
The operation being polymerized to biological sample is mainly carried out in polymerization case, but existing polymerization case does not have temperature control to set It is standby, just need to can use in addition combined with low temperature refrigerator, lower the temperature relatively difficult, refrigerating speed is difficult to ensure that, temperature can not be controlled, and existing Some polymerization casees all do not estimate impact of the ultraviolet light intensity to polymerization effect, and the sample quality made is unstable.
The content of the invention
The invention provides a kind of polymerization case, the temperature controllable precise in the polymerization case, cooling rate are fast, and can be provided with Beneficial to the stable ultraviolet light intensity of biological sample polymerization, the sample quality made using the polymerization case is stable.
The technical solution used in the present invention is:
Polymerization case, the insulation cladding body of sample room and covers the insulation cover above the insulation cladding body including offering Body, is provided with specimen holder in the sample room of the insulation cladding body, is embedded with the setting of counter sample frame in the inside of insulation lid Ultraviolet lamp house, be originally provided with refrigeration system in vivo in insulation cladding, the refrigerating evaporator in the refrigeration system is arranged on sample room On the wall of side.
Due to being originally provided with refrigeration system in vivo in insulation cladding, the refrigerating evaporator of the refrigeration system is arranged on sample room On the wall of side, thus the cryogenic temperature for adjusting refrigeration system can arrange the indoor temperature of sample room so that sample room in polymerization case Temperature controllable precise, cooling rate is fast, it can be ensured that the sample on specimen holder realizes low temperature polymerization, substantially increases biological sample The success rate of preparation.The inside of insulation lid is embedded with ultraviolet lamp house, and the ultraviolet lamp house can be biological sample polymerization in polymerization case Suitable ultraviolet light intensity is provided, the biological sample quality made is stable.
Ultraviolet lamp house includes ultraviolet lamp tube and is folded with the double-deck quartz window of heating wire, and the ultraviolet lamp tube is arranged on guarantor In the space that warm lid is surrounded with the double-deck quartz window, the ultraviolet lamp tube is located at the top of specimen holder, the bilayer quartz glass Glass window is arranged between ultraviolet lamp tube and specimen holder.The ultraviolet light that ultraviolet lamp tube sends may pass through double-deck quartz window and be radiated at On sample on sample room, heating wire is provided between double-deck quartz window, can make heating wire electrified regulation and constant temperature is to purple Temperature corresponding to outer fluorescent tube highest luminous efficiency, can meet ultraviolet lamp tube and all keep most at a temperature of -10 DEG C to -60 DEG C High luminous efficiency and there is stable light intensity, you can provides properly stable ultraviolet light intensity for sample in the case that is polymerized, preferably Guarantee the quality after sample polymerization.
The ultraviolet lamp tube is 8W365nm ultraviolet lamp tubes.Adopt the ultraviolet lamp tube of the model provide suitably for sample Ultraviolet light intensity so that polymerization effect of the sample in polymerization case is more preferable.
The refrigeration system be two grades of cascade refrigeration calculation for pipelines system of compressor, two grades of cascade refrigeration calculation for pipelines system of compressor bags Include low-temperature level pipe-line system and high-temperature level pipe-line system, the high-temperature level pipe-line system include being disposed with high-temperature level compressor, The high-temperature level refrigeration agent circulation of the evaporation tube of high-temperature level condenser, high-temperature level filter, high-temperature level capillary and condenser/evaporator Loop, low-temperature level compressor that the low-temperature level pipe-line system includes setting gradually, oil eliminator, the condensation of the condenser/evaporator The low-temperature level refrigerant circulation loop of pipe, low-temperature level filter, low-temperature level capillary and refrigerating evaporator, the oil return of oil eliminator Pipe is communicated to the working hole of low-temperature level compressor.When the refrigeration system works, high-temperature level compressor does not also have in low-temperature level compressor First work before work, by the high-temperature high-pressure gas refrigerant after high-temperature level compressor compresses from the high-pressure mouth of high-temperature level compressor Flowing out and high-temperature level condenser being reached through pipeline, after high-temperature level condenser, high-temperature high-pressure gas refrigerant is cooled into height The liquid refrigerant of pressure, enters condenser/evaporator after passing sequentially through high-temperature level filter and high-temperature level capillary filtering throttling afterwards Evaporation tube, now liquid refrigerant gasification heat absorption in the evaporation tube in condenser/evaporator, due to condenser/evaporator in it is cold Solidifying pipe and evaporation tube cooperate setting, and evaporation tube is set in outside condenser pipe, and heat transfer can occur between evaporation tube and condenser pipe, Thus now the evaporation tube absorbs heat to condenser pipe, when the condenser pipe in condenser/evaporator is cooled to -20 DEG C, low-temperature level compression Machine is powered, and now high-temperature level compressor works on;After low-temperature level compressor is powered, by the high temperature after low-temperature level compressor compresses High-pressure gas refrigerant reaches oil eliminator, high temperature and high pressure gas system from Jing pipings after the high-pressure mouth outflow of low-temperature level compressor The fluid of the refrigeration lubrication oil of the low-temperature level compressor in cryogen is separated by oil eliminator, the low-temperature level compressor after separation Refrigeration lubrication oil fluid reenter inside low-temperature level compressor by the working hole of pipeline and low-temperature level compressor, separation High-temperature high-pressure gas refrigerant afterwards is cooled into the liquid refrigerant of high pressure in low-temperature level condenser, passes sequentially through afterwards Refrigerating evaporator is entered after low-temperature level filter and low-temperature level capillary filtering throttling, into the liquid refrigeration in refrigerating evaporator Agent gasification heat absorption so that the temperature in sample room is minimized, until the temperature in sample room reaches the temperature prepared needed for sample Angle value.Simple structure, and wider refrigeration temperature levels can be provided for sample room, stable low temperature ring can be provided for sample room Border.
The sample room temperature sensor for leading to sample room is provided with insulation cladding body, in uviol lamp room is provided with lamp house temperature Degree sensor and ultraviolet light intensity sensor, the polymerization case also include controller, the controller respectively with high temperature compressor, low temperature pressure Contracting machine, heating wire, sample room temperature sensor, lamp house temperature sensor, ultraviolet light intensity sensor, ultraviolet lamp tube electrical connection. The temperature value that the controller can be collected according to sample room temperature sensor is controlling high temperature compressor, cryogenic compressor work Make, so that the temperature in sample room reaches the temperature value prepared needed for sample, the controller can also be according to lamp house TEMP Temperature value that device, ultraviolet light intensity sensor are collected and uroxameter value controlling ultraviolet lamp tube, heating wire work so that Obtain ultraviolet light intensity and reach the optimal value prepared needed for sample, be capable of achieving Automated condtrol, using more convenient.
Specimen holder side in sample room is provided with cooling circulating fan.So that sample indoor gas are able to recycle stream Move, and the temperature in sample room is quickly cooled down, and ensure that the temperature in sample room is impartial stable.
Speculum is provided with the inwall of the sample room, the ultraviolet lamp house except double-deck quartz glass outside window Speculum is provided with inwall.Ultraviolet light of the speculum to projection thereon has reflex, it is ensured that the sample on specimen holder The irradiation of ultraviolet light can fully be received.
The bottom of sample room is provided with the sample room heater strip with controller electrical connection.Sample preparation is completed Afterwards, can control sample room heater strip to heat sample room, until sample room is heated to 28 DEG C, such purpose is so that Smooth sample room will not because frosting, condensation and affect the preparation work of sample next time, if because sample room temperature is very low, After uncapping will frosting or condensation, once frosting or condensation, reflection of the speculum to ultraviolet light just greatly decline, thus can not Ensure that experiment has identical ultraviolet light intensity every time, arranges the appearance that sample room heater strip can then avoid this problem.With control Device be connected be in order to realize Automated condtrol, it is easy to operate.
Radiator fan is provided near high-temperature level condenser, the radiator fan is electrically connected with controller.Promote high temperature Level condenser radiating, it is ensured that refrigeration system is normally run.
What the present invention was brought has the beneficial effect that:
The 1st, the indoor temperature of sample room can be set so that the temperature controllable precise of sample room in polymerization case, cooling rate are fast, Can ensure that the sample on specimen holder realizes low temperature polymerization, substantially increase the success rate of biological sample preparation;
2nd, suitable ultraviolet light intensity can be provided for biological sample polymerization in the case that is polymerized, the biological sample quality made is steady It is fixed;
3rd, the Automatic Control of low temperature polymerization process is capable of achieving, is provided for Colloidal Gold Immunoelectron Microscopy Technique secure Hardware device, greatly improves the success rate that the technology prepares sample;
4th, the polymerization case is provided and is conducive to agglomeration, stable ultraviolet light intensity;
5th, the polymerization case provides wider refrigeration temperature levels, it is adaptable to more kinds of low temperature polymerization resins, there is provided Stable low temperature environment.
Description of the drawings
Overall structure diagrams of the Fig. 1 for the embodiment of the present invention;
Reference:
1st, it is incubated lid;101st, styrofoam outer layer;102nd, stainless steel speculum;2nd, insulation cladding body; 201st, styrofoam outer layer;3rd, sample room;4th, specimen holder;5th, sample room heater strip;6th, stainless steel speculum;7th, it is cold But circulating fan;8th, sample room temperature sensor;9th, ultraviolet lamp tube;10th, double-deck quartz window;11st, heating wire;12nd, lamp house Temperature sensor;13rd, ultraviolet light intensity sensor;14th, high-temperature level compressor;15th, high-temperature level condenser;16th, high-temperature level filter; 17th, high-temperature level capillary;18th, the evaporation tube of condenser/evaporator;19th, low-temperature level compressor;20th, oil eliminator;21st, condensation evaporation The condenser pipe of device;22nd, low-temperature level filter;23rd, low-temperature level capillary;24th, refrigerating evaporator;25th, radiator fan;26th, control Device;27th, silica gel sealing bar;28th, door lock;29th, lid handle;30th, hinge.
Specific embodiment
As shown in figure 1, polymerization case, including the insulation cladding body 2 for offering sample room 3 and cover in the insulation cladding body 2 The insulation lid 1 of top, in the sample room 3 of the insulation cladding body 2 is provided with specimen holder 4, is embedded in the inside of insulation lid 1 The ultraviolet lamp house for having counter sample frame 4 to arrange, is provided with refrigeration system in insulation cladding body 2, and the refrigeration in the refrigeration system is steamed Send out device 24 to be arranged on the side wall of sample room 3.
Due to being provided with refrigeration system in insulation cladding body 2, the refrigerating evaporator 24 of the refrigeration system is arranged on sample On the side wall of room 3, thus the cryogenic temperature for adjusting refrigeration system can arrange the indoor temperature of sample room 3 so that in polymerization case The temperature controllable precise of sample room 3, cooling rate are fast, it can be ensured that the sample on specimen holder 4 realizes low temperature polymerization, substantially increases Success rate prepared by biological sample.The inside of insulation lid 1 is embedded with ultraviolet lamp house, and the ultraviolet lamp house can be biological in polymerization case Sample polymerization provides suitable ultraviolet light intensity, and the biological sample quality made is stable.
Ultraviolet lamp house includes ultraviolet lamp tube 9 and is folded with the double-deck quartz window 10 of heating wire 11, and the ultraviolet lamp tube 9 sets Put in the space surrounded with the double-deck quartz window 10 in insulation lid 1, the ultraviolet lamp tube 9 is located at the top of specimen holder 4, should Double-deck quartz window 10 is arranged between ultraviolet lamp tube 9 and specimen holder 4.The ultraviolet light that ultraviolet lamp tube 9 sends may pass through bilayer Quartz window 10 is radiated on the sample on sample room 3, is provided with heating wire 11 between double-deck quartz window 10, can make to send out 11 electrified regulation of heated filament temperature corresponding to constant temperature to 9 highest luminous efficiency of ultraviolet lamp tube, can meet ultraviolet lamp tube 9- Highest luminous efficiency is all kept at a temperature of 10 DEG C to -60 DEG C and has stable light intensity, you can carry for sample in the case that is polymerized For suitable stable ultraviolet light intensity, the quality after sample polymerization is better assured that.
The ultraviolet lamp tube 9 is 8W365nm ultraviolet lamp tubes 9.Adopt the ultraviolet lamp tube 9 of the model provide properly for sample Ultraviolet light intensity so that sample polymerization case in polymerization effect it is more preferable.
The refrigeration system be two grades of cascade refrigeration calculation for pipelines system of compressor, two grades of cascade refrigeration calculation for pipelines system of compressor bags Low-temperature level pipe-line system and high-temperature level pipe-line system is included, the high-temperature level pipe-line system includes being disposed with high-temperature level compressor 14th, the high-temperature level refrigeration agent circulation of high-temperature level condenser 15, high-temperature level filter 16, high-temperature level capillary 17 and condenser/evaporator Loop, low-temperature level compressor 19 that the low-temperature level pipe-line system includes setting gradually, oil eliminator 20, condenser/evaporator, low temperature Level filter 22, the low-temperature level refrigerant circulation loop of low-temperature level capillary 23 and refrigerating evaporator 24, oil eliminator 20 are returned Oil pipe is communicated to the working hole of low-temperature level compressor 19, wherein, in high-temperature level refrigeration agent closed circuit and the condenser/evaporator Condenser pipe 21 is connected, and low-temperature level refrigerant circulation loop is connected with the evaporation tube 18 in the condenser/evaporator.The refrigeration system work When making, high-temperature level compressor 14 first worked before low-temperature level compressor 19 does not also work, after being compressed by high-temperature level compressor 14 High-temperature high-pressure gas refrigerant flow out from the high-pressure mouth of high-temperature level compressor 14 and reach high-temperature level condenser 15, Jing through pipeline After crossing high-temperature level condenser 15, high-temperature high-pressure gas refrigerant is cooled into the liquid refrigerant of high pressure, passes sequentially through height afterwards Warm level filter 16 and high-temperature level capillary 17 enter the evaporation tube 18 of condenser/evaporator after filtering throttling, now liquid refrigerant Gasification heat absorption in evaporation tube 18 in condenser/evaporator, due to the condenser pipe 21 in condenser/evaporator and 18 phase interworking of evaporation tube Close and arrange, evaporation tube 18 is set in outside condenser pipe 21, heat transfer can occur between evaporation tube 18 and condenser pipe 21, thus now should Evaporation tube 18 absorbs heat to condenser pipe 21, when the condenser pipe 21 in condenser/evaporator is cooled to -20 DEG C, low-temperature level compressor 19 It is powered, now high-temperature level compressor 14 works on;After low-temperature level compressor 19 is powered, after being compressed by low-temperature level compressor 19 High-temperature high-pressure gas refrigerant reaches oil eliminator 20 from Jing pipings after the high-pressure mouth outflow of low-temperature level compressor 19, and high temperature is high Calm the anger the low-temperature level compressor 19 in cryogen refrigeration lubrication oil fluid separated by oil eliminator 20, after separation The fluid of the refrigeration lubrication oil of low-temperature level compressor 19 reenters low temperature by the working hole of pipeline and low-temperature level compressor 19 Inside level compressor 19, the high-temperature high-pressure gas refrigerant after separation is cooled into the liquid of high pressure in low-temperature level condenser Cold-producing medium, passes sequentially through low-temperature level filter 22 afterwards and low-temperature level capillary 23 is filtered after throttling and enters refrigerating evaporator 24, Into the liquid refrigerant gasification heat absorption in refrigerating evaporator 24 so that the temperature in sample room 3 is minimized, until sample room Temperature in 3 reaches the temperature value prepared needed for sample.Simple structure, and wider cryogenic temperature can be provided for sample room 3 Scope, can provide stable low temperature environment for sample room 3.
The sample room temperature sensor 8 for leading to sample room 3 is provided with insulation cladding body 2, in uviol lamp room is provided with lamp Room temperature sensor 12 and ultraviolet light intensity sensor 13, the polymerization case also include controller 26, the controller 26 respectively with high temperature Compressor, cryogenic compressor, heating wire 11, sample room temperature sensor 8, lamp house temperature sensor 12, ultraviolet light intensity sensor 13rd, ultraviolet lamp tube 9 is electrically connected.The controller 26 can be controlled according to the temperature value collected by sample room temperature sensor 8 High temperature compressor, cryogenic compressor work, so that the temperature in sample room 3 reaches the temperature value prepared needed for sample, the control Temperature value and uroxameter value that device processed 26 can be also collected according to lamp house temperature sensor 12, ultraviolet light intensity sensor 13 Work to control ultraviolet lamp tube 9, heating wire 11, so that ultraviolet light intensity reaches the optimal value prepared needed for sample, be capable of achieving certainly Dynamicization is controlled, using more convenient.
4 side of specimen holder in sample room 3 is provided with cooling circulating fan 7.So that gas is circulated in sample room 3 Flow, and the temperature in sample room 3 is quickly cooled down, and ensure that the temperature in sample room 3 is impartial stable.
Speculum is provided with the inwall of sample room 3, in the ultraviolet lamp house in addition to double-deck quartz window 10 Speculum is provided with inwall.Ultraviolet light of the speculum to projection thereon has reflex, it is ensured that the sample on specimen holder 4 The irradiation of ultraviolet light can fully be received.
The bottom of sample room 3 is provided with the sample room heater strip 5 with the electrical connection of controller 26.Sample preparation is complete Cheng Hou, controllable sample room heater strip 5 are heated to sample room 3, until sample room 3 is heated to 28 DEG C, such purpose Be so that smooth sample room 3 will not because frosting, condensation and affect the preparation work of sample next time, if because sample room 3 Temperature is very low, after uncapping will frosting or condensation, once frosting or condensation, reflection of the speculum to ultraviolet light just greatly decline, Thus it cannot be guaranteed that experiment has identical ultraviolet light intensity every time, sample room heater strip 5 is set and can then avoid this problem Occur.Be connected with controller 26 be in order to realize Automated condtrol, it is easy to operate.
It is anti-with the stainless steel for serving as 2 inwall of insulation cladding body that insulation cladding body 2 includes styrofoam outer layer 201 Penetrate mirror 6.Insulation lid 1 includes styrofoam outer layer 101 and serves as the stainless steel speculum of insulation 1 inwall of lid 102, while the heat insulation effect for ensureing insulation cladding body 2 and insulation lid 1,6,102 pairs of throwings of stainless steel speculum of minute surface The ultraviolet light penetrated thereon has reflex so that the sample on specimen holder 4 can fully receive the irradiation of ultraviolet light, and structure Simply, it is difficult to be corroded, service life is longer.
Radiator fan 25 is provided near high-temperature level condenser 15, the radiator fan 25 is electrically connected with controller 26. High-temperature level condenser 15 is promoted to radiate, it is ensured that refrigeration system is normally run.
Silica gel sealing bar 27 is additionally provided between insulation lid 1 and insulation cladding body 2, to ensure the sealing of sample room 3, and Switchable door lock 28 is additionally provided with side of the insulation lid 1 with insulation cladding body 2, lid is additionally provided with insulation lid 1 and is drawn Hand 29, is connected by hinge 30 between insulation lid 1 and insulation cladding body 2.
The polymerization case overcomes both at home and abroad that used low temperature polymerization case can not be temperature automatically controlled, and capacity is little, poor sealing performance, Complex operation, uneasy congruence factor, is capable of achieving the Automatic Control of low temperature polymerization process, is that Colloidal Gold Immunoelectron Microscopy Technique is carried Secure hardware device has been supplied, the success rate that the technology prepares sample has been greatly improved.
The invention provides integrated, temperature and light intensity joint control, temperature can accuracy controlling freezing ultraviolet polymerization equipment;
The invention provides being conducive to agglomeration, stable ultraviolet light intensity (610 μ W/cm2);
The invention provides wider refrigeration temperature levels, it is adaptable to more kinds of low temperature polymerization resin (LR Gold zero Lower 25 DEG C, Lowicryl K4M it is subzero 35 DEG C, Lowicryl HM20 are subzero 50 DEG C), there is provided stable low temperature environment (temperature ± 1.5 DEG C of fluctuation).
Above-listed detailed description in detail is illustrating for one of present invention possible embodiments, and the embodiment is simultaneously not used to limit The scope of the claims of the present invention, all equivalence enforcements or change without departing from carried out by the present invention are intended to be limited solely by the scope of the claims of this case In.

Claims (9)

1. be polymerized case, the insulation cladding body of sample room and covers the insulation lid above the insulation cladding body including offering, Specimen holder is provided with the sample room of the insulation cladding body, it is characterised in that:It is right to be embedded with the inside of the insulation lid The ultraviolet lamp house for answering the specimen holder to arrange, is originally provided with refrigeration system, the refrigeration in the refrigeration system in vivo in the insulation cladding Evaporimeter is arranged on the side wall of the sample room.
2. polymerization case according to claim 1, it is characterised in that:The ultraviolet lamp house includes ultraviolet lamp tube and is folded with sending out The double-deck quartz window of heated filament, the ultraviolet lamp tube are arranged on the space that the insulation lid is surrounded with the double-deck quartz window Interior, the ultraviolet lamp tube is located at the top of the specimen holder, and the double-deck quartz window is arranged between ultraviolet lamp tube and specimen holder.
3. polymerization case according to claim 2, it is characterised in that:The ultraviolet lamp tube is 8W365nm ultraviolet lamp tubes.
4. the polymerization case according to claim 1 or 2 or 3, it is characterised in that:The refrigeration system is two grades of cascade refrigeration pressures Contracting machine pipe-line system, two grades of cascade refrigeration calculation for pipelines system of compressor include low-temperature level pipe-line system and high-temperature level pipe-line system, The high-temperature level pipe-line system includes being disposed with high-temperature level compressor, high-temperature level condenser, high-temperature level filter, high-temperature level hair The high-temperature level refrigeration agent closed circuit of the evaporation tube of tubule and condenser/evaporator, the low-temperature level pipe-line system include what is set gradually Low-temperature level compressor, oil eliminator, the condenser pipe of the condenser/evaporator, low-temperature level filter, low-temperature level capillary and refrigeration The low-temperature level refrigerant circulation loop of evaporimeter, the oil return pipe of oil eliminator are communicated to the working hole of low-temperature level compressor.
5. polymerization case according to claim 4, it is characterised in that:It is provided with the insulation cladding body and leads to the sample The sample room temperature sensor of room, is provided with lamp house temperature sensor and ultraviolet light intensity sensor in the uviol lamp room, and this gathers Mould assembling also include controller, the controller respectively with the high-temperature level compressor, the low-temperature level compressor, the heating wire, The sample room temperature sensor, the lamp house temperature sensor, the ultraviolet light intensity sensor, the ultraviolet lamp tube electrically connect Connect.
6. polymerization case according to claim 5, it is characterised in that:Specimen holder side in the sample room is provided with cold But circulating fan.
7. polymerization case according to claim 6, it is characterised in that:It is provided with and the control in the bottom of the sample room The sample room heater strip of device electrical connection.
8. polymerization case according to claim 7, it is characterised in that:Reflection is provided with the inwall of the sample room Mirror, removes in the ultraviolet lamp house and be provided with speculum on double-deck quartz glass inwall outside window.
9. polymerization case according to claim 8, it is characterised in that:Radiation air is provided near the high-temperature level condenser Fan, the radiator fan are electrically connected with the controller.
CN201510570733.3A 2015-09-09 2015-09-09 Polymerization tank Active CN105085719B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110186839A (en) * 2019-06-18 2019-08-30 郑州航空工业管理学院 A kind of ultraviolet accelerated photo-ageing case of pitch

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007006012A2 (en) * 2005-07-06 2007-01-11 Navigant Biotechnologies, Llc Methods for reducing pathogens in biological samples
CN201672329U (en) * 2010-05-27 2010-12-15 亚亚科技股份有限公司 Improved structure of ultraviolet light source device
CN102994385A (en) * 2012-12-12 2013-03-27 中国科学院华南植物园 Airlift optical, acoustic and magnetic control bioreactor
EP2813579A1 (en) * 2012-02-10 2014-12-17 Bioneer Corporation Apparatus and method for automatically analyzing biological samples
CN204958777U (en) * 2015-09-09 2016-01-13 中国科学院华南植物园 Polymerization case

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007006012A2 (en) * 2005-07-06 2007-01-11 Navigant Biotechnologies, Llc Methods for reducing pathogens in biological samples
CN201672329U (en) * 2010-05-27 2010-12-15 亚亚科技股份有限公司 Improved structure of ultraviolet light source device
EP2813579A1 (en) * 2012-02-10 2014-12-17 Bioneer Corporation Apparatus and method for automatically analyzing biological samples
CN102994385A (en) * 2012-12-12 2013-03-27 中国科学院华南植物园 Airlift optical, acoustic and magnetic control bioreactor
CN204958777U (en) * 2015-09-09 2016-01-13 中国科学院华南植物园 Polymerization case

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110186839A (en) * 2019-06-18 2019-08-30 郑州航空工业管理学院 A kind of ultraviolet accelerated photo-ageing case of pitch

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