CN105039155A - Real-time fluorescent quantitative PCR instrument - Google Patents
Real-time fluorescent quantitative PCR instrument Download PDFInfo
- Publication number
- CN105039155A CN105039155A CN201510455650.XA CN201510455650A CN105039155A CN 105039155 A CN105039155 A CN 105039155A CN 201510455650 A CN201510455650 A CN 201510455650A CN 105039155 A CN105039155 A CN 105039155A
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- Prior art keywords
- sample
- real
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- connection bracket
- quantitative pcr
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
- B01L7/5255—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones by moving sample containers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
Abstract
A real-time fluorescent quantitative PCR instrument comprises a case, wherein a control system connected with an upper computer is mounted in the case; a heat cycle system, a sampling part, a scanning part and a fluorescent detecting part, operated by control of the control system, are connected to the control system; the sampling part comprises a sampling motor mounted in the case and synchronous belts arranged on the two sides of the case; the synchronous belts on the two sides are connected through a rotating shaft and driven to rotate synchronously; the synchronous belts are wound on synchronous belt wheels driving the synchronous belts to move; the synchronous belt wheel on the side of the sampling motor is mounted on an output shaft of the sampling motor; push rod brackets are fixed on the synchronous belts; push rods driven by the push rod brackets to move are connected to the push rod brackets; connecting pins are mounted on the push rods; connecting brackets capable of moving up and down along the connecting pins are mounted outside the connecting pins in a sleeving manner; return springs are mounted outside the connecting pins and between the connecting brackets and the push rods in a sleeving manner; a test tube rack for placing samples is mounted between the connecting brackets on the two sides.
Description
Technical field
The invention belongs to biology and medical field, particularly relate to a kind of real-time fluorescence quantitative PCR instrument.
Background technology
Real-Time Fluorescent Quantitative PCR Technique can realize the quantitative analysis to DNA profiling, significant to molecular biology research and medical research etc.There is following defect in existing quantitative real time PCR Instrument: sample is put into detection module or is all manual when detection module takes out, and detection module temperature can be very high after analysis, can scald operator, security is not high, if operation after waiting cooling, the waiting time is oversize.
Summary of the invention
The invention provides the real-time fluorescence quantitative PCR instrument that a kind of safety performance is higher, automatization is high, reliability is high.
The technical solution used in the present invention is:
A kind of real-time fluorescence quantitative PCR instrument, comprise cabinet, the Controlling System be connected with upper computer is installed in described cabinet, described Controlling System is connected with by the heat circulating system of its controlling run, sample introduction parts, sweep unit, fluoroscopic examination parts, it is characterized in that: described sample introduction parts comprise one and are arranged on sample introduction motor in cabinet, be built up in the Timing Belt of cabinet both sides, the Timing Belt of both sides connects drive synchronous axial system by rotating shaft, described Timing Belt all installs around on the synchronous pulley driving its motion, the synchronous pulley of described sample introduction motor side is arranged on the output shaft of sample introduction motor, described Timing Belt is all fixed with pusher bar support, described pusher bar support is connected with the push rod being driven movement by it, described pusher bar support is also fixed on can along on the guide rail of the rail moving of cabinet side, described push rod is provided with joint pin, the connection bracket that can move up and down along it is set with outside described joint pin, the spring of replying is set with between the external connection bracket of described joint pin and push rod, the test-tube stand placing sample is installed between the connection bracket of both sides, after test-tube stand sample presentation puts in place, described connection bracket can be depressed and the sample on test-tube stand be entered in detection module analyze, after analysis terminates, the described connection bracket sample that appliance for releasing single rewinds on dynamic test-tube stand under the elastic force effect of spring leaves detection module.Sample introduction parts of the present invention adopt automatization form sample introduction, and sample introduction put in place after be automatically depressed in detection module, after analysis terminates, the test-tube stand of sample introduction parts automatically can rebound and make sample depart from detection module just can conveniently to take sample under the drive of connection bracket, during setting-out product, again sample is put into after opening heat lid, and do not need manually to take sample from detection module, improve safety performance, improve the reliability detected.When test-tube stand of the present invention is pressed down, pressing spring under connection bracket, after overdraft is cancelled, connection bracket is automatically rebounded under the drive of spring, and drive test-tube stand to depart from detection module, reliability is high.
Further, described heat circulating system comprises modular unit and hot cover, and described hot cover is arranged on the top of modular unit, and it comprises the heat lid that can press down test-tube stand, and described heat covers the heating film being provided with and controlling hot lid temperature.Heat circulating system adopts heat lid to keep the temperature on sample hose top.
Further, described modular unit comprises detection module, is all fixed with cooling piece, described left side scatterer, right side scatterer are all provided with fan between described detection module and left side scatterer, right side scatterer.Heat circulating system uses semiconductor chilling plate as temperature control element simultaneously, and when temperature control, if temperature is too high, dress fan on a heat sink just carries out, to scatterer blowing, achieving higher temperature-controlled precision and temperature homogeneity.
Further, described sweep unit, fluoroscopic examination parts are separately positioned on the both sides of modular unit.When sample is pressed onto in the module hole of modular unit by hot cover, sweep unit and fluoroscopic examination parts just start image data.
Beneficial effect of the present invention: safety performance is higher, automatization is high, Measurement reliability is high.
Accompanying drawing explanation
Fig. 1 is internal structure schematic diagram of the present invention.
Fig. 2 is internal structure front view of the present invention.
Fig. 3 is the structural representation of sample introduction parts of the present invention.
Fig. 4 is the structural representation of modular unit of the present invention.
Fig. 5 is the structural representation of hot cover of the present invention.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described, but does not limit the invention to these embodiments.One skilled in the art would recognize that all alternativess, improvement project and the equivalents that present invention encompasses and may comprise in Claims scope.
With reference to Fig. 1-5, a kind of real-time fluorescence quantitative PCR instrument, comprise cabinet 1, the Controlling System 2 be connected with upper computer is installed in described cabinet 1, described Controlling System 2 is connected with by the heat circulating system of its controlling run, sample introduction parts 3, sweep unit 4, fluoroscopic examination parts 5, described sample introduction parts 3 comprise one and are arranged on sample introduction motor 21 in cabinet 1, be built up in the Timing Belt 32 of cabinet 1 both sides, the Timing Belt 32 of both sides connects drive synchronous axial system by rotating shaft 34, described Timing Belt 32 all installs around on the synchronous pulley 35 driving its motion, the synchronous pulley 35 of described sample introduction motor 31 side is arranged on the output shaft of sample introduction motor 31, described Timing Belt 32 is all fixed with pusher bar support 36, described pusher bar support 36 is connected with the push rod 37 being driven movement by it, described pusher bar support 37 is also fixed on can along on the guide rail 33 of the rail moving of cabinet 1 side, described push rod 37 is provided with connection bracket 38, the test-tube stand 39 placing sample is installed between both sides connection bracket 38, after test-tube stand 39 sample presentation puts in place, described connection bracket 38 can be depressed and the sample on test-tube stand 39 be entered in detection module analyze, after analysis terminates, the sample that described connection bracket 38 energy appliance for releasing single rewinds on dynamic test-tube stand 39 leaves detection module.Sample introduction parts 3 of the present invention adopt automatization form sample introduction, and sample introduction put in place after be automatically depressed in detection module, after analysis terminates, the test-tube stand 39 of sample introduction parts 3 automatically can rebound and make sample depart from detection module just can conveniently to take sample under the drive of connection bracket 38, during setting-out product, again sample is put into after opening heat lid, and do not need manually to take sample from detection module, improve safety performance, improve the reliability detected.
Push rod 37 described in the present embodiment is provided with joint pin 30, described connection bracket 38 can be sleeved on joint pin 30 along joint pin 30 up or down, is set with the spring 11 of replying between the external connection bracket 38 of described joint pin 30 and push rod 37.When test-tube stand 39 of the present invention is pressed down, connection bracket 38 times pressing springs 11, after overdraft is cancelled, connection bracket 38 is automatically rebounded under the drive of spring 11, and drive test-tube stand to depart from detection module, reliability is high.
Heat circulating system described in the present embodiment comprises modular unit 6 and hot cover 7, and described hot cover 7 is arranged on the top of modular unit 6, and it comprises the heat lid 71 that can press down test-tube stand 38, and described heat lid 71 is provided with the heating film 72 controlling heat lid 71 temperature.Heat circulating system adopts heat lid 71 to keep the temperature on sample hose top.Heating film 72 is arranged on above heat lid 71 by heating film pressing plate 73.Heat lid 71 is provided with hot lid support 74, described hot lid support 74 is fixed on transmission screw 75, described transmission screw 75 is set with and drives its driving nut 76 moved up and down, described driving nut 76 is fixed on synchronous pulley 77, and described synchronous pulley 77 is connected with driving motor 79 by Timing Belt 78.Driving motor 79 rotates and drives Timing Belt 78 to move, Timing Belt 78 moves and drives the driving nut 76 be fixed on synchronous pulley 77 to move, driving nut 76 moves and drives transmission screw 75 to move up and down, drive the hot lid support 74 be fixed in the above to move up and down after transmission screw 75 moves, thus drive heat lid 71 to move up and down.
Described in the present embodiment, modular unit 6 comprises detection module 61, is all fixed with cooling piece 64, described left side scatterer 62, right side scatterer 63 are all provided with fan 65 between described detection module 61 and left side scatterer 62, right side scatterer 63.Heat circulating system uses semiconductor chilling plate 64 as temperature control element simultaneously, and when temperature control, if temperature is too high, dress fan 65 on a heat sink just carries out, to scatterer blowing, achieving higher temperature-controlled precision and temperature homogeneity.
Sweep unit 4 described in the present embodiment, fluoroscopic examination parts 5 are separately positioned on the both sides of modular unit 6.When sample is pressed onto in the module hole of modular unit 6 by hot cover 7, sweep unit 4 and fluoroscopic examination parts 5 just start image data.
When the present invention works, upper computer sends control command to Controlling System 2, Controlling System 2 takes orders, after the sample introduction motor 31 controlling sample introduction parts 3 rotates and is put in place by sample sample presentation, heat lid 71 is dropped to correct position by the driving motor 79 controlling hot cover 7, sample to be pressed onto in the module hole in modular unit 6 by heat lid 71 in decline process, control the work of semiconductor chilling plate 64, sample module temperature is made to meet the temperature of host computer PC R programming, Controlling System 2 gated sweep parts 4 and fluoroscopic examination parts 5 image data simultaneously, data after collection send into upper computer through Controlling System 2, upper computer receives the image data sent here, and it is analyzed, upper computer receiving control system 2 feeds back the work state information of coming simultaneously, and the working order of real-time display instrument.After detection terminates, hot cover 7 starts to control heat lid 71 and rises, and covers in 71 uphill process in heat, and because sample pulls out in module hole by the restorer of spring 11, sample is sent instrument outside by control sample introduction motor 31 by sample introduction parts 3 afterwards.
Claims (4)
1. a real-time fluorescence quantitative PCR instrument, comprise cabinet, the Controlling System be connected with upper computer is installed in described cabinet, described Controlling System is connected with by the heat circulating system of its controlling run, sample introduction parts, sweep unit, fluoroscopic examination parts, it is characterized in that: described sample introduction parts comprise one and are arranged on sample introduction motor in cabinet, be built up in the Timing Belt of cabinet both sides, the Timing Belt of both sides connects drive synchronous axial system by rotating shaft, described Timing Belt all installs around on the synchronous pulley driving its motion, the synchronous pulley of described sample introduction motor side is arranged on the output shaft of sample introduction motor, described Timing Belt is all fixed with pusher bar support, described pusher bar support is connected with the push rod being driven movement by it, described pusher bar support is also fixed on can along on the guide rail of the rail moving of cabinet side, described push rod is provided with joint pin, the connection bracket that can move up and down along it is set with outside described joint pin, the spring of replying is set with between the external connection bracket of described joint pin and push rod, the test-tube stand placing sample is installed between the connection bracket of both sides, after test-tube stand sample presentation puts in place, described connection bracket can be depressed and the sample on test-tube stand be entered in detection module analyze, after analysis terminates, the described connection bracket sample that appliance for releasing single rewinds on dynamic test-tube stand under the elastic force effect of spring leaves detection module.
2. a kind of real-time fluorescence quantitative PCR instrument as claimed in claim 1, it is characterized in that: described heat circulating system comprises modular unit and hot cover, described hot cover is arranged on the top of modular unit, it comprises the heat lid that can press down test-tube stand, and described heat covers the heating film being provided with and controlling hot lid temperature.
3. a kind of real-time fluorescence quantitative PCR instrument as claimed in claim 2, it is characterized in that: described modular unit comprises detection module, all be fixed with cooling piece between described detection module and left side scatterer, right side scatterer, described left side scatterer, right side scatterer are all provided with fan.
4. a kind of real-time fluorescence quantitative PCR instrument as claimed in claim 2 or claim 3, is characterized in that: described sweep unit, fluoroscopic examination parts are separately positioned on the both sides of modular unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510455650.XA CN105039155B (en) | 2015-07-29 | 2015-07-29 | Real-time fluorescent quantitative PCR instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510455650.XA CN105039155B (en) | 2015-07-29 | 2015-07-29 | Real-time fluorescent quantitative PCR instrument |
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| Publication Number | Publication Date |
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| CN105039155A true CN105039155A (en) | 2015-11-11 |
| CN105039155B CN105039155B (en) | 2017-01-25 |
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| CN201510455650.XA Active CN105039155B (en) | 2015-07-29 | 2015-07-29 | Real-time fluorescent quantitative PCR instrument |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400680A (en) * | 2015-12-11 | 2016-03-16 | 舟山医院 | A novel fluorescent quantitative PCR instrument |
| CN106872432A (en) * | 2017-03-30 | 2017-06-20 | 杭州晶格科学仪器有限公司 | A kind of fluorescence detection device and temperature control method with temperature control mechanism |
| CN106967585A (en) * | 2017-05-23 | 2017-07-21 | 湖州高亿诺生物科技有限公司 | For nucleic acid extraction and the integration apparatus of detection |
| CN107389961A (en) * | 2017-08-16 | 2017-11-24 | 华东医药（杭州）基因科技有限公司 | A kind of heater applied to biosystem |
| CN107460108A (en) * | 2017-07-23 | 2017-12-12 | 新疆昆泰锐生物技术有限公司 | A kind of PCR reaction systems of continuous sample introduction are prepared and sampling device and PCR instrument |
| CN108048315A (en) * | 2018-01-04 | 2018-05-18 | 深圳大学 | It is a kind of based on the fluorescence quantitative PCR instrument being automatically loaded |
| CN108587897A (en) * | 2018-05-03 | 2018-09-28 | 莫纳（苏州）生物科技有限公司 | A kind of PCR amplification device |
| CN109897781A (en) * | 2019-04-09 | 2019-06-18 | 广东省微生物研究所 | Fluorescent quantitative detection device and method |
| CN110387325A (en) * | 2019-08-13 | 2019-10-29 | 广州市华南医学研究中心 | A kind of very fast PCR reaction checking device and detection method |
| CN111902212A (en) * | 2018-02-07 | 2020-11-06 | 奥斯瓦道·克鲁兹基金会 | LAMP detection device |
| CN112924394A (en) * | 2021-01-24 | 2021-06-08 | 张苗苗 | Novel adjustable food safety inspection device |
| WO2022001990A1 (en) | 2020-06-30 | 2022-01-06 | 利多(香港)有限公司 | Internal fixing device for apparatus in transport mode, apparatus comprising internal fixing device, and method for reducing damage to apparatus during transport |
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| CN104630057A (en) * | 2015-03-05 | 2015-05-20 | 东南大学 | Full-automatic thermal cycler hot cover |
| CN204874541U (en) * | 2015-07-29 | 2015-12-16 | 杭州安杰思生物科技有限公司 | Real -time fluorescence quantitative PCR appearance |
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| CN102220225A (en) * | 2011-05-23 | 2011-10-19 | 北京工业大学 | Polymerase chain reactor and real-time electromechanical scanning and detecting device |
| CN103091298A (en) * | 2013-02-01 | 2013-05-08 | 厦门大学 | Real-time fluorescent quantitative PCR detection system |
| CN104568875A (en) * | 2014-12-22 | 2015-04-29 | 北京工业大学 | Rotary scanning real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection system |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400680A (en) * | 2015-12-11 | 2016-03-16 | 舟山医院 | A novel fluorescent quantitative PCR instrument |
| CN106872432A (en) * | 2017-03-30 | 2017-06-20 | 杭州晶格科学仪器有限公司 | A kind of fluorescence detection device and temperature control method with temperature control mechanism |
| CN106967585A (en) * | 2017-05-23 | 2017-07-21 | 湖州高亿诺生物科技有限公司 | For nucleic acid extraction and the integration apparatus of detection |
| CN106967585B (en) * | 2017-05-23 | 2024-03-12 | 湖州师范学院 | Integrated device for nucleic acid extraction and detection |
| CN107460108A (en) * | 2017-07-23 | 2017-12-12 | 新疆昆泰锐生物技术有限公司 | A kind of PCR reaction systems of continuous sample introduction are prepared and sampling device and PCR instrument |
| CN107389961B (en) * | 2017-08-16 | 2019-11-08 | 领航基因科技（杭州）有限公司 | A kind of heating device applied to biosystem |
| CN107389961A (en) * | 2017-08-16 | 2017-11-24 | 华东医药（杭州）基因科技有限公司 | A kind of heater applied to biosystem |
| CN108048315A (en) * | 2018-01-04 | 2018-05-18 | 深圳大学 | It is a kind of based on the fluorescence quantitative PCR instrument being automatically loaded |
| CN111902212A (en) * | 2018-02-07 | 2020-11-06 | 奥斯瓦道·克鲁兹基金会 | LAMP detection device |
| CN108587897A (en) * | 2018-05-03 | 2018-09-28 | 莫纳（苏州）生物科技有限公司 | A kind of PCR amplification device |
| CN108587897B (en) * | 2018-05-03 | 2021-08-10 | 莫纳（苏州）生物科技有限公司 | PCR amplification device |
| CN109897781A (en) * | 2019-04-09 | 2019-06-18 | 广东省微生物研究所 | Fluorescent quantitative detection device and method |
| CN110387325A (en) * | 2019-08-13 | 2019-10-29 | 广州市华南医学研究中心 | A kind of very fast PCR reaction checking device and detection method |
| WO2022001990A1 (en) | 2020-06-30 | 2022-01-06 | 利多(香港)有限公司 | Internal fixing device for apparatus in transport mode, apparatus comprising internal fixing device, and method for reducing damage to apparatus during transport |
| CN112924394A (en) * | 2021-01-24 | 2021-06-08 | 张苗苗 | Novel adjustable food safety inspection device |
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| CN105039155B (en) | 2017-01-25 |
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Address after: Jianggan District Danong Port Road Hangzhou city Zhejiang province 310044 1216 2 Building No. 6 room 607 Patentee after: Hangzhou an reputation Technology Co., Ltd. Address before: Hangzhou City, Zhejiang Province, 310000 Xihu District three Town Xiyuan eight road No. 11 Building 2, 3 floor 310 Patentee before: HANGZHOU ANJIESI BIOTECHNOLOGY CO., LTD. |
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