CN105011974A - Method and device for welding biological tissue by mixed laser beam - Google Patents
Method and device for welding biological tissue by mixed laser beam Download PDFInfo
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- CN105011974A CN105011974A CN201510419803.5A CN201510419803A CN105011974A CN 105011974 A CN105011974 A CN 105011974A CN 201510419803 A CN201510419803 A CN 201510419803A CN 105011974 A CN105011974 A CN 105011974A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/00491—Surgical glue applicators
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/00491—Surgical glue applicators
- A61B2017/005—Surgical glue applicators hardenable using external energy source, e.g. laser, ultrasound
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/00491—Surgical glue applicators
- A61B2017/00504—Tissue welding
- A61B2017/00508—Tissue welding using laser
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/00491—Surgical glue applicators
- A61B2017/00513—Tissue soldering
- A61B2017/00517—Tissue soldering using laser
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Abstract
The invention provides a method and device for welding a biological tissue by a mixed laser beam. The method provided by the invention comprises the steps that: two or more lasers with different wave lengths are coupled into one welding beam to irradiate the to-be-welded surface of the biological tissue, and the lasers with different wave lengths are selectively absorbed in tissue thickness direction and generate the photothermal effect; the light spot diameter of the welding beam on the to-be-welded tissue surface is adjusted through a focusing system; the moving route and speed of the welding beam on the to-be-welded tissue surface is controlled through a scanning system; the temperature of the welding area is detected through a photoelectric detector, and the moving speed of the welding beam is adjusted according to the temperature. According to the differences in the optical properties of different tissues or different parts of the same tissue in a organism, the lasers with two or more wave lengths are matched and coupled into one beam to irradiate the surface of the biological tissue, the deep seam welding of the full layer of the incision in the tissue is realized, the mixed laser with different output modes can be selected to irradiate the incision or the wounded tissue, and the size of the irreversible thermal damage tissue is effectively reduced.
Description
Technical field
The invention belongs to laser medicine field, be specifically related to a kind of method and device of mixed light beam laser weld biological tissue.
Background technology
Nearest decades, scientific research personnel research and development efficient suture material and suture instruments in achieve remarkable achievement, in succession develop degradable and containing antibacterial stitching thread, the mechanical type anastomat, tissue adhesive etc. of taking out stitches and shorten suture time can be simplified.Simultaneously suture instruments has started to develop to Wicresoft, automation direction, but this kind of apparatus exists certain shortcoming, as expensive, and easily kish nail etc. in vivo; Tissue adhesive then need further raising in adhesion strength, biocompatibility, degradability etc.
Along with the development of laser technology, it is also constantly expanded in the application of medical field.Laser biology tissue welding has the advantages such as operating time is short, wound healing is fast, inflammatory reaction is little, cicatrix is not obvious as a kind of Minimally Invasive Surgery, and be a kind of tissue apposition substitute technology of having very much potentiality, its some clinical practices have obtained the accreditation of people.Utilize laser action to have a large amount of clinical demand in the biological tissue that biological tissue produces photo-thermal effect principle solder technology of coincideing, but laser biology tissue welding technology mainly concentrate on experimental study and the clinical practice of single wavelength at present.In single wavelength laser biology tissue welding device, output be the hot spot of fixed dimension mostly, the irradiation being difficult to meet different large minimal incision * or wound surface with cure requirement.Simultaneously, the optical characteristics of biological tissue because of classification different with position, and differ greatly, as 980nm laser is mainly absorbed by skin surface, comparatively small part is absorbed by deep tissues, and 1064nm laser can go deep into deep skin tissue, therefore the identical welding that single wavelength laser beam is realizing biological tissue has obvious limitation, easily causes otch discontinuity in the depth direction to be coincide.Current Ye You double light beam laser biological tissue solder technology, but its two bundles laser is separated, and has and focus on complicated shortcoming, and be difficult to Collaborative Control two and restraint the exposure dose of laser on otch or wound surface and mobile route.
Summary of the invention
The object of the present invention is to provide a kind of method and device of mixed light beam laser weld biological tissue, can according to organism different tissues or the same optical characteristics difference organizing different parts, mate the laser of two kinds or more of wavelength and make it to synthesize a branch of welding laser irradiating biological tissue, realizing the identical welding object of biological tissue's otch or the wound surface holostrome degree of depth; The present invention also can select the hybrid laser of different output mode to irradiate otch or wound tissue, can effectively reduce irreversible hot injury and organize area.
In order to solve the problems of the technologies described above, the invention provides a kind of method of mixed light beam laser weld biological tissue, the laser coupled two or more with different wave length is expose to tissue surface to be welded after a branch of welding light beam, and the laser of different wave length is produced photo-thermal effect by selective absorbing on tissue thickness direction.
Further, focusing system is used to adjust the spot diameter of described welding light beam irradiation at tissue surface to be welded.
Further, scanning system is used to control welding light beam in the mobile route of tissue surface to be welded and speed.
Further, use photodetector to gather the temperature of welding region, control the translational speed of welding light beam according to described temperature, welding region temperature is remained in reasonable interval.
Further, be a branch ofly weld light beam by pilot light and the laser coupled of described different wave length, described pilot light is used to indicate the irradiation position of described welding light beam.
Further, according to physical characteristic and the optical characteristics of biological tissue to be welded, determine wavelength and the Laser output pattern of each laser; According to size and the degree of depth of tissue cut to be welded or wound surface, set the translational speed etc. of the power of each laser, repetition rate, pulsewidth and welding light beam.The present invention, according to the optical characteristics of biological tissue to be welded, mates the laser instrument of exportable specific wavelength light beam; According to the otch of biological tissue to be welded or the size of wound surface and the degree of depth, under known organism organizes the physical characteristic such as specific heat capacity, density, rough estimation biological tissue reaches the total heat input needed for 60 ~ 70 DEG C, then the translational speed of the peak power of laser instrument, pulsewidth and repetition rate and welding light beam is regulated, to obtain required heat input, make tissue degeneratiaon to be welded and condensation to reach identical object.Pulsewidth length major effect pulsed light beam is to the heat effect effect of biological tissue, and peak power and spot diameter one timing, it is different that distinct pulse widths makes biological tissue be warming up to maximum temperature required time.Repetition rate is the pulse number of output beam in the unit time, affects the interval time of pulse, if the pulse spacing is shorter, the heat effect of adjacent beams pulse to tissue has superposed contributions and dependency; If the interval long enough of pulse, during the pulse spacing, the temperature investigating point will drop to initial temperature, and the variations in temperature that so next light beam pulse causes is almost identical with the effect of a upper light beam pulse.Under other parameter one stable condition, translational speed is faster, and the thermal energy of the absorption of biological tissue is fewer.The output mode of laser instrument have continuous laser and pulse laser output point, the such as mixed model of strobe pulse laser and continuous laser, continuous wave laser ensures that light beam provides Continuous Heat input quantity in biological tissue, biological tissue's temperature is made to improve 10 ~ 20 DEG C, weaken biological tissue's enzymatic activity, fixed cell tissue; Pulse laser can accurately control the heat effect of light beam in biological tissue further by adjusting pulsewidth and repetition rate, biological tissue's temperature is made to be increased to 60 ~ 70 DEG C, protein and collagen protein generation degeneration and condensation, under pulse spacing conservative control, biological tissue can obtain short time cooling, instead of persistently overheatingly cause tissue film to penetrate or decomposition of vaporizing, therefore the mixing bond pattern of pulse laser and continuous laser has the therapeutic effect reducing irreversible hot injury biological tissue area.
Further, form the mobile route of described welding light beam according to the otch of tissue to be welded or wound surface extension direction, according to mobile route, welding light beam is acted on biological tissue position to be welded by scanning system.
The present invention also provides a kind of device of mixed light beam laser weld biological tissue, comprises laser generator system, laser bundling device, laser aligner, focusing system and scanning system; Described laser generator system comprises two or more laser instrument, for launching the laser of different wave length; It is a branch of welding light beam that described laser bundling device is used for the laser coupled of described different wave length; Described focusing system is for adjusting the spot diameter of described welding light beam irradiation at tissue surface to be welded; Described scanning system welds light beam in the mobile route of tissue surface to be welded and speed for controlling.
Further, in described laser generator system, one of them laser instrument is microlaser, for exporting pilot light, and the irradiation position of instruction welding light beam.
Further, described device also comprises photodetector and control system, and described photodetector is for gathering the temperature signal of welding region, and described control system controls the translational speed of welding light beam according to described temperature signal.
Laser generator system exports some specific wavelength laser beams, it is a branch of that laser bundling device can make multiple laser be coupled in, then by focusing system and scanning system, certain size hot spot is irradiated to otch or the wound surface of biological tissue, the temperature signal that photodetector gathers otch or wound tissue welding region feeds back in control system, on-line control translational speed, finally reaches that hot injury is little, the high and restorative fast high-quality welding effect of seriality of coincideing.In addition, multiple laser be coupled in a branch of after, compare and spatially control two bundle laser respectively, more easily focus, the mobile route of light beam more easy to operate on otch or wound tissue and speed, shorten weld interval.
Compared with prior art, its remarkable advantage is in the present invention:
(1) light beam coupling of different wave length is become beam of laser by the present invention, when irradiating otch or wound tissue, meet the difference requirements of biological tissue's different optical characteristic, the laser of different wave length and the different depth position of tissue can be made to interact and to produce photo-thermal effect, to realize coincideing continuously of depth direction;
(2) the present invention can select the mixed model of different Laser output pattern, and pulse laser is compared continuous laser and had the therapeutic effect reducing irreversible hot injury biological tissue area;
(3) in the present invention, mixing multiple laser be coupled in a branch of after, compare the laser irradiating biological tissue be separated with multi beam, be easy to keep the light beam of different wave length to concentrate on the same position on otch or wound tissue surface simultaneously, make the control of mixed light beam mobile route simple;
(4) the present invention introduces miniature laser generator transmitting pilot light, pilot light and main laser beam synthesize, be convenient to find the hot spot original position of otch or the wound surface irradiated in biological tissue, shorten weld interval, instruction red light wavelength λ is 650nm, output is less than 6mW, can ignore, and welding process can close this laser power supply on the impact of test or postoperative outcome;
(5) spot diameter of focusing system adjustable mixed light beam, realizes continuously adjustabe in 0.5 ~ 5mm, meets the welding object of different large minimal incision *, compares the laser irradiation tissue that two bundles are separated, decrease a focusing system and regulate convenient;
(6) scanning system can realize the basic figures such as straight line, circle, polygon under control system controls, what substantially meet the otch in difformity and space or wound tissue welds object, and accessible site is in control system, online quick adjustment light beam translational speed;
(7) when biological tissue arrives uniform temperature, the structure such as collagen or albumen starts degeneration and solidifies, two broken ends of fractured bone are made to organize Adherents junction, the present invention uses photodetector to gather the optical signalling of welding region, convert temperature signal to, feed back to control system, adjustment drive circuit changes light beam translational speed, the temperature that biological tissue is welded is in optimum range, welding region high-temperature residence time is reduced, the welding effect that the identical seriality of final realization is high and irreversible hot injury's area is little while ensureing basic welding temperature.
Accompanying drawing explanation
Fig. 1 is the apparatus structure schematic diagram of the present invention's mixed light beam laser weld biological tissue.
Fig. 2 is the scanning system structural representation in device of the present invention.
Fig. 3 is the method flow diagram of the present invention's mixed light beam laser weld biological tissue.
Fig. 4 is the light beam mobile route schematic diagram in the embodiment of the present invention.
Detailed description of the invention
Easy understand, according to technical scheme of the present invention, when not changing connotation of the present invention, one of ordinary skill in the art can be imagined and the method for the present invention's mixed light beam laser weld biological tissue and the numerous embodiments of device.Therefore, following detailed description of the invention and accompanying drawing are only the exemplary illustrations to technical scheme of the present invention, and should not be considered as of the present invention all or the restriction be considered as technical solution of the present invention or restriction.
Composition graphs 1, the present embodiment mixed light beam laser weld biological tissue device, mainly comprises laser generator system 1, output optical fibre 2,3 × 1 laser bundling devices 3, laser aligner 4, focusing system 5, scanning system 6, photodetector 10, control system 11.
Laser generator system 1 carries three laser instrument, and wherein the first laser instrument 1-1 and second laser 1-2 exports the laser of different wave length, and the pulse laser of different wave length or continuous wave laser can be adopted to realize; 3rd laser instrument 1-3 be microlaser for exporting HONGGUANG, instruction welding light beam irradiation is in the position of target otch or wound surface.The laser that laser generator system 1 is launched gives laser bundling device 3 by output optical fibre 2.
Laser bundling device 3 becomes beam of laser for the laser coupled of being launched by above-mentioned three laser instrument, and export under the combined effect of collimator 4 and focusing system 5 and have certain diameter and the little light beam of the angle of divergence, then this light beam enters scanning system 6.
The structure of scanning system 6 as shown in Figure 2, form primarily of two light scanning lens, X-scan module, Y-scan module, X-drive circuit and Y-drive circuit, control system 11 drives X-scan module, Y-scan module respectively by X-drive circuit and Y-drive circuit, thus in the deflection of X-Y plane internal control controlling laser beam, complete the mobile route of light beam on two dimensional surface, this scanning system can realize the basic figures such as straight line, circle, polygon under control system 11 controls, and meets difformity and welds object with the otch in space or the identical of wound tissue.
Control system 11 is for the mobile route of predetermined beam and speed, and welding light beam 7 is acted on target otch or the wound surface of biological tissue 8 according to described mobile route and speed by gated sweep system 6.
Photodetector 10 is for gathering the optical signal of biological tissue's welding region in welding process and being converted into temperature signal, control system 11 pairs of temperature signals are analyzed, if welding region temperature does not reach preset value, then slowed down by drive circuit and weld the translational speed of light beam 7, if exceed preset value, then accelerated the translational speed of welding light beam 7 by drive circuit.
Composition graphs 3, in conjunction with said apparatus, the method for the present invention's mixed light beam laser weld biological tissue is:
Step 1, according to the physical characteristic of different biological tissue and optical characteristics, welding light beam launched by the laser generator of coupling specific wavelength, and select the output mode of laser instrument, hybrid laser output mode comprises continuously and pulse mixing output mode, mix output mode, pulse and pulse mixing output mode etc. continuously and continuously.The optical characteristics difference of biological tissue's different parts is larger, such as, for skin histology, 980nm and 1064nm laser is easy to be absorbed by skin epidermis and skin corium (or deep layer) respectively, therefore 980nm continuous wave laser and 1064nm pulse laser can be selected, by a branch of for the Laser synthesizing of these two kinds of wavelength irradiation tissue, mainly act on epidermal area and the skin corium (or deep layer) of skin respectively, produce photo-thermal effect and make tissue degeneratiaon and condensation, then can realize the identical welding object of the skin incision holostrome degree of depth.980nm continuous wave laser parameter mainly power, 1064nm pulse laser parameter mainly peak power, pulsewidth and repetition rate etc.Continuous wave laser ensures that light beam provides Continuous Heat input quantity in tissue, makes tissue temperature improve 10 ~ 20 DEG C, weakens histaminase activity, fixed cell tissue; Pulse laser can accurately control light beam in in-house heat effect further by adjusting pulsewidth and repetition rate, tissue temperature is made to be increased to 60 ~ 70 DEG C, protein and collagen protein generation degeneration and condensation, under pulse spacing conservative control, tissue can obtain short time cooling, instead of persistently overheatingly cause tissue film to penetrate or decomposition of vaporizing, therefore the mixing bond pattern of pulse laser and continuous laser has the therapeutic effect reducing irreversible hot injury biological tissue area.
Step 2, according to appearance such as the otch of tissue or the size of wound tissue, the degree of depth and areas, under the physical characteristic such as specific heat capacity, density of known tissue, the total heat input reached needed for 60 ~ 70 DEG C is organized in rough estimation, the parameter of the first laser instrument 1-1 and second laser 1-2 is preset, export the welding light beam that tissue degeneratiaon is condensed, and estimate the translational speed of welding light beam.Continuous wave laser parameter is mainly power, and pulse laser parameter mainly comprises peak power, pulsewidth and repetition rate etc.Power plays most important impact to heat input, and power is high, organizes temperature rise fast; Power is low, otherwise then.This pulsed light beam of pulsewidth length major effect is to the heat effect effect of tissue, and peak power and spot diameter one timing, it is different that distinct pulse widths makes tissue be warming up to maximum temperature required time.Repetition rate is the pulse number of output beam in the unit time, affects the interval time of pulse, if the pulse spacing is shorter, the heat effect of adjacent beams pulse to tissue has superposed contributions and dependency; If the interval long enough of pulse, during the pulse spacing, the temperature investigating point will drop to initial temperature, and the variations in temperature that so next light beam pulse causes is almost identical with the effect of a upper light beam pulse.Under other parameter one stable condition, translational speed is faster, and the thermal energy of the absorption of biological tissue is fewer; Translational speed is slower, then the tissue hyperthermia time of staying is long, and irreversible hot injury's area of generation may be larger.
Step 3, according to tissue otch or wound surface size, modulation focusing system 5, setting welding light beam 7 spot diameter;
Step 4, placement biological organization sample, in work platforms 9, utilize miniature laser generator 1-3 to export instruction HONGGUANG, utilize control system 11 to find the original position of the hot spot irradiating destination organization;
Step 5, according to tissue otch or wound surface extension direction, under the guiding of HONGGUANG, control system 11 use programming software preset welding light beam mobile route;
After step 6, above-mentioned all optimum configurations, close miniature laser generator 1-3, open the power supply of the first laser instrument 1-1 and second laser 1-2, operation control system 11, welding light beam 7 starts to carry out laser weld to destination organization otch or wound surface 8;
In step 7, laser beam welding, photodetector 10 gathers welding region optical signal, converts temperature signal to 11 times, and judge whether to reach preset value in control system, if do not reach, control drive circuit slows down and welds the translational speed of light beam; Exceed preset value certain limit, then accelerate translational speed;
Step 8, welding process are complete, and shutdown system power supply, removes destination organization.
Laser aligner 4 in above-mentioned steps 3 and focusing system 5, can carry out shaping to the light beam after bundling device 3 coupling, export diameter at the continuously adjustable light beam of 0.5 ~ 5mm scope, and the angle of divergence of light beam be little as far as possible.It is 650nm that miniature laser generator 1-3 in above-mentioned steps 4 exports red light wavelength λ, and output is less than 6mW, and welding process can be ignored to Influence on test result.In above-mentioned steps 5, scanning system 6 can realize " spot scan " and " line sweep " two kinds of patterns of light beam, for the wound tissue that area is less, can select " spot scan " pattern; For longer tissue cut, " line sweep " pattern can be selected.
Below in conjunction with experiment, the present invention will be further described.
Experiment 1
To be with the fresh in vitro Carnis Sus domestica of full thickness skin, sample-size 100 × 100 × 10mm, prepare the long otch of 50mm of 3 interval 20mm with scalpel, the degree of depth crosses whole bark layer (comprise epidermis and corium, about 1.5mm is thick).On every bar otch solder side, the equidistant suture of 3 pin is avoided opening excessively affect laser weld to be drawn otch.
The basic parameter of design welding light beam, in laser generator system, 1064nm pulse laser 1-1 peak power arranges 5W, pulsewidth 10ms, repetition rate 20HZ, and output is then that 1W, 980nm continuous wave laser 1-2 output is set to 1W.Regulate focusing system 5, make output facula diameter reach 2mm.
Design light beam mobile route and speed, be positioned over sample tissue on workbench 9 also fixing, open miniature laser generator 1-3 power supply, export HONGGUANG, find the original position of light beam at otch.The computer control software of operation control system 11, according to otch extension direction, the mobile route of editor's welding light beam and speed, as Fig. 4, bold portion is the mobile route of light beam in cut surface, speed 2mm/s; Dotted portion be light beam at the mobile route organizing normal portions surface, speed 30mm/s, Given this light beam translational speed in region is very fast, therefore can ignore actual result impact.After mobile route and Speed Setting, close the power supply of miniature laser generator 1-3, start the first laser instrument 1-1 and second laser 1-2, operation control system 11, welding light beam 7 starts to carry out laser weld to destination organization otch 8.
In welding process, photodetector 10 gathers welding region optical signal, under control system, convert temperature signal to, and judges whether to reach 60 ~ 70 DEG C, if do not reach, compensates calculating, controls drive circuit and to slow down light beam translational speed; If exceed 60 ~ 70 DEG C, then accelerate light beam translational speed, whole process keeps target area temperature within the scope of 60 ~ 70 DEG C.
After welding, all power supplys of shutdown system, remove sample tissue.
Experiment 2
For the sciatic nerve of live body rat.
The basic parameter of design welding light beam, in laser generator system, the He-Ne laser instrument 1-2 of coupling wavelength to be the argon ion laser 1-1 of 514.5nm and wavelength be 633nm, two laser instrument all export continuous laser.Argon ion laser 1-1 power setting 500mW, He-Ne laser instrument 1-2 power setting 200mW.Regulate focusing system 5, make output facula diameter reach 1mm.
After the anesthesia of live body rat, depilation, routine disinfection, cuts skin and Musclar layer, free sciatic nerve, and section neatly cuts off nerve trunk wherein, under operating microscope, then makes nerve segment end involutory, and by a small amount of droplet of blood obtained from Mus tail in identical end.White mouse in special cage internal fixtion, and is placed on workbench 9, opens miniature laser generator 1-3 power supply, exports HONGGUANG, and adjustment facula position makes it the impact point be accurately radiated in nerve trunk.The computer control software of operation control system 11, the speed of editor's welding light beam is 0 (i.e. spot scan).Close miniature laser generator 1-3 power supply, start the first laser instrument 1-1 and second laser 1-2, operation control system 11, welding light beam 7 starts to carry out laser weld to tissue cut 8.
In welding process, photodetector 10 gathers welding region optical signal, temperature signal is converted under control system, and be presented at the display screen of control system 11, manual observation display screen, if reach 60 ~ 70 DEG C, then modulate the position of cage or sciatic nerve-trunk, make welding light beam 7 be radiated at the more lower of nerve trunk otch, final irradiate 4 points, 90 °, each some interval.
After welding, all power supplys of shutdown system, remove experiment sample.
Experiment 3
For the liver of common domesticated dog.
Cut open the belly to after the anesthesia of common live body domesticated dog, expose liver, linearly cut 1 long 30mm, the otch of dark 5mm with scalpel on its surface, on otch solder side, sewing up a pin affects laser weld in centre to draw otch and avoid opening excessive.
The basic parameter of design welding light beam, in laser generator system, the continuous wave laser 1-2 of coupling wavelength to be the Nd:YAG pulse laser 1-1 of 1064nm and wavelength be 1350nm.Laser instrument 1-1 peak power arranges 10W, pulsewidth 20ms, repetition rate 20HZ, and output is then that 4W, laser instrument 1-2 output is set to 2W.Regulate focusing system 5, make output facula diameter reach 3mm.
Design light beam mobile route and speed, be positioned on workbench 9 also fixing by domesticated dog together with liver organization, open miniature laser generator 1-3 power supply, export HONGGUANG, find the original position of light beam at otch.The computer control software of operation control system 11, according to otch extension direction, the mobile route of editor's welding light beam and speed, welding light beam implements " spot scan " to otch, overlapping 1mm between 2, the irradiation time of often is 10s, and the speed that light beam moves to next point from previous point is 10mm/s.After mobile route and Speed Setting, close the power supply of miniature laser generator 1-3, start the first laser instrument 1-1 and second laser 1-2, operation control system 11, welding light beam 7 starts to carry out laser weld to destination organization otch 8.
In welding process, photodetector 10 gathers welding region optical signal, under control system, convert temperature signal to, and judges whether to reach 60 ~ 70 DEG C, if do not reach, compensates calculating, controls drive circuit and to slow down light beam translational speed; If exceed 60 ~ 70 DEG C, then accelerate light beam translational speed, whole process keeps target area temperature within the scope of 60 ~ 70 DEG C.
After welding, all power supplys of shutdown system, remove experiment sample.
Claims (10)
1. the method with mixed light beam laser weld biological tissue, it is characterized in that, the laser coupled two or more with different wave length is expose to tissue surface to be welded after a branch of welding light beam, and the laser of different wave length is produced photo-thermal effect by selective absorbing on tissue thickness direction.
2. use the method for mixed light beam laser weld biological tissue as claimed in claim 1, it is characterized in that, use focusing system to adjust the spot diameter of described welding light beam irradiation at tissue surface to be welded.
3. use the method for mixed light beam laser weld biological tissue as claimed in claim 1, it is characterized in that, use scanning system to control described welding light beam in the mobile route of tissue surface to be welded and speed.
4. use the method for mixed light beam laser weld biological tissue as claimed in claim 1, it is characterized in that, use photodetector to gather the temperature of welding region, according to the translational speed of described temperature adjustment welding light beam, welding region temperature is remained in reasonable interval.
5. as described in claim 1,2,3 or 4, use the method for mixed light beam laser weld biological tissue, it is characterized in that, be a branch ofly weld light beam by pilot light and the laser coupled of described different wave length, described pilot light is used to indicate the irradiation position of described welding light beam.
6. use the method for mixed light beam laser weld biological tissue as claimed in claim 5, it is characterized in that, according to physical characteristic and the optical characteristics of biological tissue to be welded, determine wavelength and the Laser output pattern of each laser; According to size and the degree of depth of tissue cut to be welded or wound surface, set the translational speed etc. of the power of each laser, repetition rate, pulsewidth and welding light beam.
7. use the method for mixed light beam laser weld biological tissue as claimed in claim 5, it is characterized in that, form the mobile route of described welding light beam according to the otch of tissue to be welded or wound surface extension direction, according to mobile route, welding light beam is acted on biological tissue position to be welded by scanning system.
8. with a device for mixed light beam laser weld biological tissue, it is characterized in that, comprise laser generator system, laser bundling device, laser aligner, focusing system and scanning system;
Described laser generator system comprises two or more laser instrument, for launching the laser of different wave length;
It is a branch of welding light beam that described laser bundling device is used for the laser coupled of described different wave length;
Described focusing system is for adjusting the spot diameter of described welding light beam irradiation at tissue surface to be welded;
Described scanning system welds light beam in the mobile route of tissue surface to be welded and speed for controlling.
9. device as claimed in claim 1, it is characterized in that, in described laser generator system, one of them laser instrument is microlaser, for exporting pilot light, the irradiation position of instruction welding light beam.
10. device as claimed in claim 1, is characterized in that, also comprise photodetector and control system, and described photodetector is for gathering the temperature signal of welding region, and described control system controls the translational speed of welding light beam according to described temperature signal.
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| CN107308553A (en) * | 2017-06-13 | 2017-11-03 | 华中科技大学 | A kind of Intelligent Laser therapeutic system for Wound treating |
| CN112535531A (en) * | 2020-11-27 | 2021-03-23 | 广东省医疗器械研究所 | Method and device for detecting welding effect of biological tissue |
| CN112842520A (en) * | 2021-01-30 | 2021-05-28 | 南京理工大学 | Laser biological tissue welding seam self-adaptive path planning device and method thereof |
| CN112957124A (en) * | 2021-01-30 | 2021-06-15 | 南京理工大学 | Laser biological tissue welding and suturing method with energy targeted regulation and control |
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