CN104977343A - Efficient biosensor based on graphene/mesoporous carbon nano composite material and preparation method of efficient biosensor - Google Patents
Efficient biosensor based on graphene/mesoporous carbon nano composite material and preparation method of efficient biosensor Download PDFInfo
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- CN104977343A CN104977343A CN201510437055.3A CN201510437055A CN104977343A CN 104977343 A CN104977343 A CN 104977343A CN 201510437055 A CN201510437055 A CN 201510437055A CN 104977343 A CN104977343 A CN 104977343A
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Abstract
The invention provides a biosensor based on a graphene/mesoporous carbon nano composite material and a preparation method of the biosensor. The graphene/mesoporous carbon nano composite material is prepared by adopting a hydrothermal synthesis method and is used as an adsorption enzyme immobilized material; a bio-sensing and electrochemical principle is adopted, and a method combining silk screen printing and ink jet printing is used for manufacturing detection test paper; and the silk screen printing is used for printing a conductive circuit, and a sensitive biological element is jet-printed on an electrode supporting object by adopting a non-contact spraying manner, wherein the spraying amount and the spraying area of a spraying material can be controlled. A nano composite carrier material is mesoporous carbon grown on the two surfaces of a graphene sheet layer, so that the graphene/mesoporous carbon nano composite material is prepared; and the graphene/mesoporous carbon nano composite material is used as a carrier immobilized enzyme to be physically mixed with a biological enzyme solution, and the biological enzyme solution is jet-printed and modified on a glassy carbon electrode by an ink jet printer to be used for rapidly and efficiently detecting blood glucose.
Description
Technical field
The invention belongs to the multi-crossed disciplines fields such as printed electronics, nano material technology, biotechnology, be specifically related to a kind of high performance biosensors based on Graphene/mesoporous carbon nano composite material and preparation method thereof.
Background technology
Diabetes have become Community health's problem, and global onset diabetes rate rapid development, diabetes have become the chronic disease of the third-largest serious threat human health after tumour, cardiovascular pathological changes.Current global diabetic oneself more than 1.2 hundred million people, China patient numbers occupies the second in the world, oneself break through 2,000 ten thousand.Estimate according to the World Health Organization (WHO), by 2025, global maturity-onset diabetes patient numbers will increase to 300,000,000, and diabetes mellitus in China patient numbers will reach 4,000 ten thousand, and in coming few decades, diabetes will be Chinese serious public health problems.
In modern medical service detection field, instant detection is developed rapidly, and the development of this technology makes patient just can carry out self-examination at home.Glucose biological sensor directly can need not carry out pre-service to blood to whole blood test, because of easy to carry, highly sensitive, selectivity is good, detection speed is fast, simple to operate and system has the features such as intelligent, average family can be entered carry out at any time and long-term monitoring blood, there is good prospect.
Summary of the invention
Technical matters to be solved by this invention is to provide a kind of high performance biosensors based on Graphene/mesoporous carbon nano composite material and preparation method thereof.Graphene/mesoporous carbon nano composite material can overcome the drawback of general nano material; the activity of efficient maintenance enzyme and the quick transmission of electronics can be carried out; in conjunction with ink-jet technology; the biology sensor of preparation can obtain test result quickly and easily in conjunction with supporting instrument; and method for making is simple; facilitate post-production and encapsulation, be conducive to large-scale production.
Technical matters solved by the invention can realize by the following technical solutions.
A kind of biology sensor detecting glucose, by flexible substrates, print working electrode on a flexible substrate and electrode is formed, described working electrode is a kind of bilayer conductive film, be made up of the biology enzyme electrically conductive ink on water-soluble conducting ink layer and its, described biology enzyme electrically conductive ink is made up of Graphene/mesoporous carbon nano composite material, glucose oxidase and Nafion filmogen.
Described flexible substrates adopts the polymeric material that a kind of good toughness, insulativity are high, as Polyvinylchloride, polyester or polycarbonate.
Above-mentioned biology sensor, working electrode is coated with biology enzyme electrically conductive ink.Can catalysis glucose response, also can carry out swift electron transmission between enzymic catalytic reaction center and electrode by adsorptive enzyme again, so form a circuit loop, according to the height of the concentration of glucose in blood during reaction, produce the current signal of corresponding size, and detected reading by corresponding tester.
Based on a biology sensor preparation method for Graphene/mesoporous carbon nano composite material, it comprises the following steps:
1) conductive black, graphite, water-borne acrylic resin, deionized water, 25 ~ 28wt% ammoniacal liquor are fully mixed with the mass ratio of 10:5:15:15:3 prepare water-soluble conducting ink, for subsequent use;
2) metal of 200-400 order number or nylon polyesters material web are fixed on square light-weight metal framework, in darkroom, coat photoresists, drying for standby;
3) by the working electrode of computer drawing be placed on photoresists to electrode negative film, under 20-40 watt of ultraviolet, expose 15-30 minute, rinse the unhardened photoresists going to be covered by electrode negative film with giant, after drying, form screen template;
4) by with working electrode be installed on screen process press to the screen template of electrode pattern, water-soluble conducting ink is adopted to print working electrode, to electrode;
By Rate activity be 5) >=glucose oxidase of 150u/mg is dissolved in the phosphate buffer of pH7.0 and is mixed with the enzyme solutions that concentration is 10mg/ml, shelves 0 DEG C of refrigerator for subsequent use after fully dissolving;
6) using Graphene/mesoporous carbon nano composite material as carrier material, join in enzyme solutions, mix and blend 10 ~ 36h, for adsorbing glucose oxidase, for subsequent use;
7) the Nafion filmogen solution that massfraction is 0.5% is prepared, add in the obtained mixed solution of step 6), mix and blend 30min, obtained biology enzyme electrically conductive ink, then the mode of spraying is adopted to be printed onto working electrode surface, under room temperature, dry 24h can complete the modification of the immobilized of enzyme and electrode, makes biology sensor.
The preparation concrete steps of Graphene in such scheme step 6)/mesoporous carbon nano composite material are:
The molecular guide agent of getting 1.5 ~ 2g is dissolved in the deionized water mix and blend 10 ~ 30min of 10 ~ 30ml, and the concentration adding 1 ~ 2ml is the HCl continuation stirring of 1M; Add the Graphene of 30 ~ 50mg and the beta-schardinger dextrin-of 1.5 ~ 2g successively; stir 0.5 ~ 1h; subsequently mixed liquor to be transferred in reactor thermal treatment reaction 24 ~ 36h at 120 ~ 150 DEG C; under the protection of last nitrogen in tubular furnace; the programming rate of 10 DEG C/min to reaction 3 ~ 5h at 700 ~ 800 DEG C, nano composite material.
The preparation of described Graphene, utilizes cheap electrically conductive graphite starting material, adopts electrophoresis, by adjusting the composition, pH value, impressed voltage value etc. of electrophoresis liquid, few layer (<10 layer) Graphene of separating high-purity high conductivity.It is characterized in that, electrophoretic voltage is set to 60-100V; Described Graphene refers to any one in single-layer graphene, few layer graphene (< 10) or their potpourri.
The present invention adopts serigraphy to print conducting wire, does conducting effect, thus reduces the requirement of technique making, and wire printing material and screen printing device need not specially be processed, and can be obtained by commercially available channel, and cost is very low; Adopt electrophoresis from graphite, extract few layer of graphite of preparation good conductivity, simple, starting material are easy to get, and cost is lower; Specific surface area is large, the Graphene/mesoporous carbon nano composite material of good biocompatibility, good conductivity to adopt hydrothermal synthesis method to prepare, not only can immobilized glucose oxidase, modify on electrode simultaneously and accelerate the transmission of electronics between enzyme active center and electrode, increase the sensitivity of sensor; Adopt spraying printing technology to control quantity for spray and the area of biological sensitive materials, the precision of final products and repeatability are fully ensured.
Beneficial effect of the present invention is:
1) serigraphy and spraying is adopted to print the method combined, silk-screen printing technique process can not only be simplified, reduce costs, composite filament India side formula can be eliminated again to the adverse effect of biological sensitive materials-enzyme, the mode of printing of spraying effectively can control quantity for spray and the spray area of biological sensitive materials-enzyme, whole technological operation is simple, with low cost, can carry out large-scale production;
2) from graphite cheap and easy to get, electrophoresis goes out few layer graphene, and simple with the existing method ratio method preparing Graphene, cost is low, and obtained graphene conductive performance is good;
3) adopt Graphene/mesoporous carbon nano composite material of preparing of hydro-thermal method, there is good biocompatibility, poriness and specific surface area is large etc. that advantage is conducive to the immobilized of enzyme, and the interface resistance of compound substance is little, good conductivity, is conducive to the transfer promoting electronics;
4) utilize the graphene composite material modified electrode of good conductivity, be conducive to the sensitivity of increase biology sensor, response time and sensing range.This invention greatly can promote the detection of Portable household blood sugar concentration.
Accompanying drawing explanation
Fig. 1 is biology sensor Test paper structural drawing prepared by the present invention;
Fig. 2 is biological sensor electrode schematic diagram prepared by the present invention;
Fig. 3 is the preparation flow figure of biological sensor electrode prepared by the present invention;
Fig. 4 is Graphene/mesoporous carbon composite material fundamental diagram.
Embodiment
Further illustrate below in conjunction with embodiment.
Embodiment 1
1) take the graphite of 10g, the conductive black of 5g, the water-borne acrylic resin of 15g, the deionized water of 15g, the ammoniacal liquor mixed grinding stirring 1h of 3g, prepare water-soluble conducting ink, for subsequent use.
2) 200 object metals or nylon polyesters material are fixed on square light-weight metal framework, in darkroom, coat Kodak's photoresists, drying for standby;
3) by the working electrode of computer drawing be placed on photoresists to electrode negative film, expose 15 minutes under 25 watts of uviol lamps, rinse the unhardened photoresists going to be covered by electrode negative film with giant, after dry, form silk-screen template;
4) by with working electrode be installed on (SYP) type screen printer to the screen template of electrode pattern, water-soluble conducting ink is adopted to print working electrode, to electrode;
By Rate activity be 5) >=glucose oxidase of 150u/mg is dissolved in the phosphate buffer of pH7.0 and is mixed with the enzyme solutions that concentration is 10mg/ml, shelves 0 DEG C of refrigerator after fully dissolving, for subsequent use;
6) the few layer graphene of preparation: surfactant, ammonia conduction liquid and water are made into electrolytic solution by a certain percentage, two panels copper sheet is wired on the positive and negative electrode of electrophoresis apparatus, distance about 10 ~ 15cm between upper and lower two electrodes, electrophoretic voltage is set to 60-100V, ionizes;
7) prepare Graphene/mesoporous carbon composite material: the molecular guide agent of getting 1.5g--triblock copolymer F127 is dissolved in the deionized water for stirring 10min of 20ml, the concentration adding 1ml is the HCl continuation stirring of 1M; Add right 2 step 2 of 30mg successively) beta-schardinger dextrin-of the Graphene prepared and 1.5g, stirs 0.5h.Subsequently mixed liquor to be transferred in reactor thermal treatment reaction 24h at 130 DEG C, finally in tubular furnace nitrogen protection under, the programming rate of 10 DEG C/min reacts 3h at 750 DEG C, and obtained nano composite material is for subsequent use.
8) Graphene step 7) prepared/mesoporous carbon nano composite material joins in step 5) enzyme solutions as carrier material, mix and blend absorption 10h; The Nafion filmogen solution being 0.5% by preparation massfraction joins in obtained mixed solution, mix and blend 30min, adopt spraying method by gained mixed solution, be printed onto on working electrode, under room temperature, dry 24h can complete the modification of the immobilized of enzyme and electrode, the obtained high performance biosensors based on Graphene/mesoporous carbon.
Embodiment 2
The present embodiment is roughly the same with embodiment 1, and difference is, the impressed DC voltage in step 6) during the few layer graphene of electrophoretic preparation increases to 100V, and other condition is constant.
Embodiment 3
The present embodiment is roughly the same with embodiment 1, and difference is, in step 7), in tubular furnace, calcining heat is raised to 800 DEG C, and other condition is constant.
Embodiment 4
The present embodiment is roughly the same with embodiment 1, and difference is, the adsorption time of the nano composite material in step 8) to enzyme rises to 24h, and other condition is constant.
The present invention adopts the method for serigraphy to prepare circuit and naked carbon electrode, and cost of manufacture is low, convenient and easy; Ink jet printing mode is adopted to be printed by biology enzyme when the size of the amount of sprayed on material and covering effectively can be controlled; Two kinds of carbon nanomaterials with high biology enzyme adsorptive power and high conductivity are combined with each other simultaneously, are conducive to the fast transfer of electronics, realize the highly sensitive detection of biology sensor.The mode of surperficial direct growth is utilized to modify Graphene, not only remain the schistose texture of Graphene two dimension and high electric conductivity, overcome the difficult point of nano composite material height interface resistance, and solve the problem of easily reuniting between graphene sheet layer, the good biological microenvironment that the poriness of the mesoporous carbon simultaneously grown provides for enzyme, is conducive to the immobilized of enzyme.In addition, the fixing matrix being suitable for different scale biomolecule can be prepared by the aperture etc. regulating and controlling mesoporous carbon, promote that heterogeneous electron shifts and improves the biological electro catalysis performance of immobilized protein, this makes mesoporous carbon valuable in redox protein matter Direct Electrochemistry, has widened the evolutionary path of carbon back biology sensor simultaneously.
Claims (5)
1. one kind is detected the biology sensor of glucose, it is characterized in that, by flexible substrates, print working electrode on a flexible substrate and electrode is formed, described working electrode is a kind of bilayer conductive film, be made up of the biology enzyme electrically conductive ink on water-soluble conducting ink layer and its, described biology enzyme electrically conductive ink is made up of Graphene/mesoporous carbon nano composite material, glucose oxidase and Nafion filmogen.
2. biology sensor according to claim 1, is characterized in that, described flexible substrates adopts Polyvinylchloride, polyester or polycarbonate.
3. the preparation method of biology sensor according to claim 1, is characterized in that comprising the following steps:
1) conductive black, graphite, water-borne acrylic resin, deionized water, 25 ~ 28wt% ammoniacal liquor are fully mixed with the mass ratio of 10:5:15:15:3 prepare water-soluble conducting ink, for subsequent use;
2) metal of 200-400 order number or nylon polyesters material web are fixed on square light-weight metal framework, in darkroom, coat photoresists, drying for standby;
3) by the working electrode of computer drawing be placed on photoresists to electrode negative film, under 20-40 watt of ultraviolet, expose 15-30 minute, rinse the unhardened photoresists going to be covered by electrode negative film with giant, after drying, form screen template;
4) by with working electrode be installed on screen process press to the screen template of electrode pattern, water-soluble conducting ink is adopted to print working electrode, to electrode;
By Rate activity be 5) >=glucose oxidase of 150u/mg is dissolved in the phosphate buffer of pH7.0 and is mixed with the enzyme solutions that concentration is 10mg/ml, shelves 0 DEG C of refrigerator for subsequent use after fully dissolving;
6) using Graphene/mesoporous carbon nano composite material as carrier material, join in enzyme solutions, mix and blend 10 ~ 36h, for adsorbing glucose oxidase, for subsequent use;
7) the Nafion filmogen solution that massfraction is 0.5% is prepared, add in the obtained mixed solution of step 6), mix and blend 30min, obtained biology enzyme electrically conductive ink, then the mode of spraying is adopted to be printed onto working electrode surface, under room temperature, dry 24h can complete the modification of the immobilized of enzyme and electrode, makes biology sensor.
4. preparation method according to claim 3, is characterized in that, in step 6), the preparation process of Graphene/mesoporous carbon nano composite material is:
The molecular guide agent of getting 1.5 ~ 2g is dissolved in the deionized water mix and blend 10 ~ 30min of 10 ~ 30ml, and the concentration adding 1 ~ 2ml is the HCl continuation stirring of 1M; Add the Graphene of 30 ~ 50mg and the beta-schardinger dextrin-of 1.5 ~ 2g successively; stir 0.5 ~ 1h; subsequently mixed liquor to be transferred in reactor thermal treatment reaction 24 ~ 36h at 120 ~ 150 DEG C; under the protection of last nitrogen in tubular furnace; the programming rate of 10 DEG C/min to reaction 3 ~ 5h at 700 ~ 800 DEG C, nano composite material.
5. preparation method according to claim 4, is characterized in that, described Graphene adopts electrically conductive graphite starting material, adopts electrophoresis preparation.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105597803A (en) * | 2015-12-14 | 2016-05-25 | 江南大学 | Mesoporous carbonitride photocatalyst and preparation method thereof |
| CN105699453A (en) * | 2016-01-24 | 2016-06-22 | 西南大学 | Preparation method and application of ink jet printing plane three-electrode system |
| CN105973962A (en) * | 2016-04-25 | 2016-09-28 | 陈前伟 | Preparation method of glucose sensor based on graphene nano wall |
| CN107167503A (en) * | 2017-05-27 | 2017-09-15 | 山东农业大学 | A kind of chloramphenicol electrochemical sensor detection method |
| CN107966478A (en) * | 2016-10-19 | 2018-04-27 | 华邦电子股份有限公司 | Sensor array, its manufacture method and method for sensing |
| CN111803087A (en) * | 2020-06-12 | 2020-10-23 | 同济大学 | Organism nondestructive blood sugar detection device and preparation method thereof |
| CN111855774A (en) * | 2020-05-19 | 2020-10-30 | 大连理工大学 | Prussian blue-based intelligent wearable enzyme-based biosensor and preparation method and application thereof |
| CN114609204A (en) * | 2022-03-15 | 2022-06-10 | 肇庆学院 | CMK-8 and GNs combined modified electrode, electrochemical sensor and application |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1922478A (en) * | 2004-02-23 | 2007-02-28 | 香港澳维有限公司 | Microfluidic test device |
| CN101091114A (en) * | 2004-08-31 | 2007-12-19 | 生命扫描苏格兰有限公司 | Method of manufacturing an auto-calibrating sensor |
| CN102472719A (en) * | 2009-06-30 | 2012-05-23 | 雅培糖尿病护理公司 | Analyte monitoring device and methods of use |
| US20130034664A1 (en) * | 2008-04-14 | 2013-02-07 | Nigel John Forrow | Biosensor Coating Composition and Methods Thereof |
| CN102998348A (en) * | 2012-11-27 | 2013-03-27 | 重庆医科大学 | Preparation method of dehydrogenase-electrochemical biosensor |
| CN104020206A (en) * | 2014-06-10 | 2014-09-03 | 上海大学 | Graphene/ordered-mesoporous-carbon-modified carbon paste electrode for electrochemical detection of estrogen and application thereof |
-
2015
- 2015-07-23 CN CN201510437055.3A patent/CN104977343B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1922478A (en) * | 2004-02-23 | 2007-02-28 | 香港澳维有限公司 | Microfluidic test device |
| CN101091114A (en) * | 2004-08-31 | 2007-12-19 | 生命扫描苏格兰有限公司 | Method of manufacturing an auto-calibrating sensor |
| US20130034664A1 (en) * | 2008-04-14 | 2013-02-07 | Nigel John Forrow | Biosensor Coating Composition and Methods Thereof |
| CN102472719A (en) * | 2009-06-30 | 2012-05-23 | 雅培糖尿病护理公司 | Analyte monitoring device and methods of use |
| CN102998348A (en) * | 2012-11-27 | 2013-03-27 | 重庆医科大学 | Preparation method of dehydrogenase-electrochemical biosensor |
| CN104020206A (en) * | 2014-06-10 | 2014-09-03 | 上海大学 | Graphene/ordered-mesoporous-carbon-modified carbon paste electrode for electrochemical detection of estrogen and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王琨琦: "介孔碳修饰玻碳电极上葡萄糖氧化酶的直接电化学", 《电化学》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105597803A (en) * | 2015-12-14 | 2016-05-25 | 江南大学 | Mesoporous carbonitride photocatalyst and preparation method thereof |
| CN105597803B (en) * | 2015-12-14 | 2017-11-03 | 江南大学 | A kind of mesoporous carbon nitride photocatalyst and preparation method thereof |
| CN105699453A (en) * | 2016-01-24 | 2016-06-22 | 西南大学 | Preparation method and application of ink jet printing plane three-electrode system |
| CN105973962A (en) * | 2016-04-25 | 2016-09-28 | 陈前伟 | Preparation method of glucose sensor based on graphene nano wall |
| CN107966478A (en) * | 2016-10-19 | 2018-04-27 | 华邦电子股份有限公司 | Sensor array, its manufacture method and method for sensing |
| CN107167503A (en) * | 2017-05-27 | 2017-09-15 | 山东农业大学 | A kind of chloramphenicol electrochemical sensor detection method |
| CN111855774A (en) * | 2020-05-19 | 2020-10-30 | 大连理工大学 | Prussian blue-based intelligent wearable enzyme-based biosensor and preparation method and application thereof |
| CN111803087A (en) * | 2020-06-12 | 2020-10-23 | 同济大学 | Organism nondestructive blood sugar detection device and preparation method thereof |
| CN111803087B (en) * | 2020-06-12 | 2021-11-09 | 同济大学 | Organism nondestructive blood sugar detection device and preparation method thereof |
| CN114609204A (en) * | 2022-03-15 | 2022-06-10 | 肇庆学院 | CMK-8 and GNs combined modified electrode, electrochemical sensor and application |
| CN114609204B (en) * | 2022-03-15 | 2023-09-15 | 肇庆学院 | CMK-8 and GNs combined modified electrode, electrochemical sensor and application |
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