CN104944515B - A kind of highly effective continuous frequency mixing ultrasonic breaking-wall cell processing unit - Google Patents
A kind of highly effective continuous frequency mixing ultrasonic breaking-wall cell processing unit Download PDFInfo
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- CN104944515B CN104944515B CN201510399857.XA CN201510399857A CN104944515B CN 104944515 B CN104944515 B CN 104944515B CN 201510399857 A CN201510399857 A CN 201510399857A CN 104944515 B CN104944515 B CN 104944515B
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Abstract
The present invention relates to a kind of highly effective continuous frequency mixing ultrasonic breaking-wall cell processing unit, including breaking-wall cell ultrasonic reaction cavity, the two ends of reaction cavity are respectively charging aperture and discharging opening；The ultrasonic reaction cavity includes the high power altrasonic transducer that at least two different frequencies are provided with the cylinder reaction chamber that several are connected by pipeline, each cylinder reaction chamber.Pending sample is diluted to liquid and is pumped into by flow control valve by reaction vessel charging aperture, is detoured in reaction cavity.High power altrasonic transducer is placed in the insertion of reaction chamber both sides face-to-face, and effect can be achieved ultrasonic wave mixing to two ultrasonic transducers face-to-face in each group, and the ultrasonic wave of synthesis can realize different frequency and the sound intensity, reach the continuous, purpose of high-efficiency wall breaking.Ultrasonic cell means for breaking walls proposed by the present invention is simple to operate, be easily achieved, efficiency high, continuation strong, has higher application value to the break process of the biological cells such as algae.
Description
Technical field
The present invention relates to sonication techniques field, more particularly to a kind of highly effective continuous frequency mixing ultrasonic breaking-wall cell processing
Device.
Background technology
With the development of social economy, environmental problem has put on political affairs at different levels as a unavoidable major issue
The agenda at mansion.Environmental protection, mitigation environmental pollution, containment ecological degeneration trend turn into important of government's social management
Business.In numerous pollution control research, as algae and water plant is spread unchecked, it is necessary to destroy the plant cell wall of algae, make thin
Intracellular organic matter flows out, to reach the purpose for administering algae pollution；It is a large amount of due to existing in sludge in some sludge handling process
Biological cell, its moisture combined in a variety of forms make it that the water content of sludge is very big, it is therefore desirable at biological cell
Reason, separates out its moisture including intracellular water combined, so as to subsequent treatment, reaches the purpose for reducing sludge moisture content.
Therefore, the research of biological cell broken wall causes the great attention of scientific worker, how to continue efficiently to carry out at biological cell
Reason turns into important research topic.
In recent years, ultrasonic technology has obtained effective application in environmental improvement, is referred to as environment-friendly technology.Greatly
Amplitude ultrasonic energy is concentrated, and may be such that medium produces high vibration, in terms of being usually used in being cleaned by ultrasonic, emulsify, being chemically treated.
Ultrasonic wave is made up of a series of compressional wave of density interphases, and is propagated by liquid medium to surrounding.When acoustic energy is made with some strength
It for fluid molecule, can make it that the attraction between liquid phase molecule is broken, form cavitation nucleus.The life-span of cavitation nucleus is about 0.1 micro-
Second, it can produce about 400k localized hyperthermia and 100MPa partial high pressure environment in instant of detonation, while producing speed
About 400km/h has the microjet of strong impacts, and this phenomenon is referred to as ultrasonic cavitation.These conditions are enough to make organic matter exist
Chemical bond fission, pyrolytic, aqueous phase burning or radical reaction occur in cavitation bubble, therefore ultrasonic wave can be applied effectively
Among the application of breaking-wall cell.
Ultrasonic wave, which abolishes biological cell mechanism, destruction cell membrane, destruction gas vacuole, destruction organized enzyme etc..Experiment has proven to height
The ultrasonic wave energy destruction cell membrane of intensity, flows out intracellular matter.For example：For algae, the special tectonic of its cell
It is that cell body contains multigroup gas vacuole, gas vacuole controls the elevating movement of alga cells.Shock wave, jet, radiation caused by ultrasonic wave
Pressure etc. can destroy gas vacuole.Under appropriate frequency, gas vacuole turns into cavitation bubble and ruptured；Meanwhile, the HTHP that cavitation is produced
With a large amount of free radicals, intracellular reactive enzyme and active material can be destroyed, so as to influence the Physiology and biochemistry activity of cell.
At present, the ultrasonic processing apparatus of various biological cell broken walls occurs in succession, but the following problem of generally existing：
Ultrasonic transducer selection is single, and ultrasonic power and frequency have been fixed, and carrying out broken wall to different biological cells can not realize simultaneously；
Combined echocardiography is acted on other chemical methods, although is improved broken wall efficiency, but is caused secondary pollution problems；Ultrasonic power
Small, sporoderm-broken rate is relatively low, although can effectively suppress Growth of Biologic Cell, but can not fundamentally destroy cell, cause sample treatment
Efficiency does not reach industrial requirement.Therefore, it is badly in need of considering factors above, designs a kind of highly effective continuous ultrasonic cell broken wall
Processing unit.
When biological cell is by two or more different frequency ul-trasonic irradiations, the ultrasonic wave of different frequency can be to aquatic products
Raw different cavitation effect, and can be produced during the ultrasonic wave mixing of multi-frequency by approach such as frequency multiplication, frequency dividings complicated and changeable
Frequency and produce more cavitation effects；Secondly, the frequency mixing ultrasonic ripple of two kinds of frequencies can reduce the production of single-frequency ultrasonic wave
Raw standing wave, and standing wave can caused cavitation cave reduction；Again, the biological cell of different type and size surpasses to different frequency
Phonoreception sensitivity is different, and frequency mixing ultrasonic frequency complicated and changeable is brighter than the ultrasonic effect for individually applying specific frequency
It is aobvious.It therefore, it can solve the problem of existing Vltrasonic device is present at present by frequency mixing ultrasonic technology.
The content of the invention
It is an object of the invention to provide a kind of frequency mixing ultrasonic breaking-wall cell processing unit, efficient and continuous welding is reached
The effect of cell membrane in pollutant.
Realizing the technical scheme of above-mentioned purpose is：A kind of highly effective continuous frequency mixing ultrasonic breaking-wall cell processing unit, including
Breaking-wall cell ultrasonic reaction cavity, the two ends of ultrasonic reaction cavity are respectively charging aperture and discharging opening；The ultrasonic reaction cavity
Including the cylinder reaction chamber that several are connected by pipeline, the high-power of at least two different frequencies is provided with each cylinder reaction chamber
Ultrasonic transducer.Wherein described reaction cavity is made up of two or more cylinder reaction chamber, is connected, treated by pipeline between reaction chamber
Processing sample is diluted to liquid and is pumped into by flow control valve by reaction vessel charging aperture, is detoured in reaction cavity, and via
Container discharge mouth flows out naturally, into follow-up processing system.In the ultrasonic cylinder reaction chamber both sides insertion of described breaking-wall cell
The high power altrasonic transducer placed face-to-face, ultrasonic transducer is replaceable, here from three kinds of ultrasonic transducer frequency difference
For 20kHz, 28kHz and 35kHz, for different sample transducer power outputs between 500W-4kW.These three frequencies it is super
Sonic transducer is that low frequency is conventional, high power can be achieved, to the experiment proved that and can produce frequency complicated and changeable and generation by mixing
Different cavitation effects acts on different biological cells.Ultrasonic transducer frequency 20kHz and 28kHz be one group, 28kHz and
35kHz is one group, and the achievable ultrasonic wave of effect is mixed face-to-face for two ultrasonic transducers in one group, each group by 20kHz and 35kHz
Frequently, the ultrasonic wave of synthesis can realize different frequency and the sound intensity.
The equipment is in processing sample, and sample is in flow regime, and by ultrasonic continuous action, so that should
Device can carry out effective integration with other equipment, realize the continuous processing of sample；The large power supersonic being fixed in equipment is changed
Energy device can be changed according to the species of processing sample, the ultrasound of flexibly selection different capacity and frequency be realized, so as to same
When to different biological cells carry out broken wall treatment；Selected ultrasonic transducer is high power device, biological cell wall is realized effective
Broken wall, improves efficiency.It is demonstrated experimentally that for different biological cells, using the sporoderm-broken rate of the disposable continuous processing sample of the device
40%-70% is reached, the disposal ability of sample is up to 100-300m3/ hour, handling sample by equipment cascading mode can reach
To higher broken wall efficiency, so as to reach industrial treatment requirement.
Further, the high power altrasonic transducer is made up of ultrasonic transducer and ultrasonic amplitude transformer, and is each surpassed
Sonic transducer one supersonic generator of correspondence.
Further, the high power altrasonic transducer selects power bracket between 100W-4kW as needed, frequency
20kHz and 28kHz is one group, and 28kHz and 35kHz are one group, and 20kHz and 35kHz are one group, and the power and mixing scope are enough
Various biological cell walls are crushed.
Further, the reaction cavity is made up of three cylinder reaction chambers, is connected between reaction chamber by pipeline, can be with
Install and change high power altrasonic transducer, fixed using flange, the maintenance to ultrasonic transducer can be facilitated, and can root
According to biological cell species and size selection different capacity and the ultrasonic transducer of frequency.
Further, the breaking-wall cell ultrasonic reaction cavity can pass through discharging opening according to processing sample size and species
Multiple cascades are carried out with charging aperture.
Further, the breaking-wall cell ultrasonic reaction cavity charging aperture installs flow control valve, and flow control valve can be with
Flow velocity of the pending sample in reaction cavity is controlled, so as to control the ultrasonic action time to sample.
Further, the breaking-wall cell ultrasonic reaction cavity charging aperture is provided with discharging provided with charging aperture valve, discharging opening
Mouth valve.
Particularly advantage of the invention is：The present invention provides a kind of highly effective continuous frequency mixing ultrasonic breaking-wall cell processing unit,
Existing ultrasonication device can be overcome the shortcomings of, ultrasonic cavitation efficiency can be improved, ultrasonic standing wave generation is reduced, ultrasound is realized
Different biological cell membranes can be carried out broken wall treatment, ultrasonic cell means for breaking walls behaviour proposed by the present invention by continuously, efficiently effect
Make it is simple, be easily achieved, it is strong using safety, efficiency high, continuation, have to administering the ecological problems caused by biological cell such as blue-green algae
Very high engineering application value, is also with a wide range of applications in some other bioengineering and environmental area.
Brief description of the drawings
Fig. 1 is the structural representation of the ultrasonic cell broken wall treatment device of the present invention.Have in figure：Breaking-wall cell ultrasonic reaction
Cavity 1,20kHz high power altrasonic transducers 2,28kHz high power altrasonic transducers 3,35kHz high power altrasonic transducers 4, stream
Control valve 5, charging aperture 6, charging aperture valve 7, discharging opening 8, discharging opening valve 9.
Embodiment
The device includes the part such as breaking-wall cell ultrasonic reaction cavity, high power altrasonic transducer, flow control valve and constituted：
Wherein reaction cavity 1 is made up of three cylinder reaction chambers, is connected between reaction chamber by pipeline, including discharging opening 8, charging aperture 6,
Jack, discharging opening valve 9 and charging aperture valve 7 for inserting high power altrasonic transducer 2,3,4；High power altrasonic transducer
Container is inserted by the jack of reaction cavity and fixed with flange；Flow control valve 5 is installed on charging aperture, controls pending sample
Flow velocity in reaction cavity, so as to control the ultrasonic action time to sample.High power altrasonic transducer is switching on power simultaneously
Apply after signal, ultrasonic wave mixing can be achieved in effect to two ultrasonic transducers face-to-face in each group, and the ultrasonic wave of synthesis can be real
Existing different frequency and the sound intensity, and generation ultrasound cavitation effect acts on biological cell in specific region.Before device work first
High power altrasonic transducer is inserted into container by the jack of reaction cavity and fixed with flange, discharging opening valve is closed, from entering
Material mouth water filling, the full whole container of filling, power-on and transmitting signal makes high power altrasonic transducer work.Now open discharging
Mouthful valve and after pending sample is diluted by flow control valve from charging aperture injects reaction cavity, pass through flow control valve
Flow velocity of the pending sample in reaction cavity can be controlled, so as to control the ultrasonic action time to sample.Pending sample
Continuously across ultrasonication, efficient, continuous broken wall purpose is realized, and from discharging opening outflow, into aftertreatment systems.
Below by taking blue-green algae as an example, the present invention is elaborated with reference to concrete condition.
Embodiment 1
Tetra- groups of concentration identical each 200ml of microcystic aeruginosa liquid of A, B, C, D are taken, ultrasonic power output is 500W, A, B, C group
Be respectively that the ultrasonic of 20KHz, 28KHz and 35KHz is acted on frequency, exposure time be 15 seconds, D groups respectively with 20kHz with
28kHz is mixed, and 28kHz and 35kHz mixing, 20kHz and 35kHz are mixed each effect 5 seconds, and the algae solution through ultrasonication is statically placed in
In incubator, take supernatant to measure absorbance (OD values), contrasted with the control group without ultrasonication, so as to quantitatively obtain
The biological content of blue-green algae in solution, as shown in table 1.
Table 1
| Control group | A groups | B groups | C groups | D groups | |
| Supersonic frequency (KHz) | 20 | 28 | 35 | Mixing | |
| Ultrasonic power (kW) | 0.5 | 0.5 | 0.5 | 0.5 | |
| Action time (Sec) | 15 | 15 | 15 | 15 | |
| OD values | 0.450 | 0.279 | 0.266 | 0.288 | 0.212 |
| Biological content | 62% | 59% | 64% | 47% |
As it can be seen from table 1 being handled using frequency mixing ultrasonic blue-green algae, the biology that can effectively reduce blue-green algae in solution contains
Amount, so as to improve clearance, the removal effect of blue-green algae can improve 25% or so.
Embodiment 2
Tetra- groups of concentration identical each 200ml of microcystic aeruginosa liquid of A, B, C, D are taken, ultrasonic power output is adjusted to 500W, four groups
Frequency mixing ultrasonic is used, exposure time is respectively 15 seconds, 20 seconds, 25 seconds and 30 seconds, and the algae solution through ultrasonication is statically placed in into training
Support in case, take supernatant to measure absorbance (OD values), contrasted with the control group without ultrasonication, so as to quantitatively obtain molten
The biological content of blue-green algae in liquid, as shown in table 2.
Table 2
| Control group | A groups | B groups | C groups | D groups | |
| Supersonic frequency (KHz) | Mixing | Mixing | Mixing | Mixing | |
| Ultrasonic power (kW) | 0.5 | 0.5 | 0.5 | 0.5 | |
| Action time (Sec) | 15 | 20 | 25 | 30 | |
| OD values | 0.450 | 0.212 | 0.189 | 0.176 | 0.167 |
| Biological content | 47% | 42% | 39% | 37% |
From table 2 it can be seen that blue-green algae is handled using frequency mixing ultrasonic, with the increase of frequency mixing ultrasonic action time,
The biological content reduction of blue-green algae in solution, corresponding clearance rise, because this method is designed as continuous treatment process, Bu Huiwu
The limit rise ultrasonication time, therefore reasonable time scope can be selected as needed.
Embodiment 3
Take tetra- groups of concentration identical each 200ml of microcystic aeruginosa liquid of A, B, C, D, ultrasonic power output be respectively 0.5kW,
1kW, 2kW, 3kW, are acted on 15 seconds using frequency mixing ultrasonic, the algae solution through ultrasonication are statically placed in incubator, takes supernatant to survey
Absorbance (OD values) is measured, is contrasted with the control group without ultrasonication, so that the biology for quantitatively obtaining blue-green algae in solution contains
Amount, as shown in table 3.
Table 3
| Control group | A groups | B groups | C groups | D groups | |
| Supersonic frequency (KHz) | Mixing | Mixing | Mixing | Mixing | |
| Ultrasonic power (kW) | 0.5 | 1 | 2 | 3 | |
| Action time (Sec) | 15 | 15 | 15 | 15 | |
| OD values | 0.450 | 0.212 | 0.171 | 0.140 | 0.117 |
| Biological content | 47% | 38% | 31% | 26% |
From table 3 it can be seen that being handled using frequency mixing ultrasonic blue-green algae, with the increase of frequency mixing ultrasonic power, solution
The biological content of middle blue-green algae is substantially reduced, corresponding clearance rise.
Claims (4)
1. a kind of highly effective continuous frequency mixing ultrasonic breaking-wall cell processing unit, including breaking-wall cell ultrasonic reaction cavity (1), ultrasound
The two ends of reaction cavity (1) are respectively charging aperture (6) and discharging opening (8)；Characterized in that, ultrasonic reaction cavity (1) bag
Include the large power supersonic transducing provided with two different frequencies in three cylinder reaction chambers connected by pipeline, each cylinder reaction chamber
Device；The high power altrasonic transducer is made up of ultrasonic transducer and ultrasonic amplitude transformer, and each ultrasonic transducer correspondence one
Individual supersonic generator；The high power altrasonic transducer selects power bracket between 100W-4kW as needed；
When high power altrasonic transducer provided with two different frequencies in each cylinder reaction chamber, three cylinder reaction chambers difference
The combination of frequency of the ultrasonic transducer used for：Frequency 20kHz and 28kHz are one group, and 28kHz and 35kHz are one group, 20kHz
It it is one group with 35kHz；
Three cylinder reaction chambers for constituting ultrasonic reaction cavity (1) are the mode being connected in series, i.e.,：First cylinder reaction chamber
One end is provided with the charging aperture (6) of ultrasonic reaction cavity (1), and the discharging opening of the first cylinder reaction chamber connects the reaction of the second cylinder
The charging aperture of chamber, the discharging opening of the second cylinder reaction chamber connects the charging aperture of the 3rd cylinder reaction chamber, the reaction of the 3rd cylinder
The discharging opening of chamber is the discharging opening (8) of ultrasonic reaction cavity (1)；
One end fixed frequency for closing on charging aperture of first cylinder reaction chamber is 28kHz high power altrasonic transducer, and this first
Discharging opening one end of closing on of cylinder reaction chamber is provided with the high power altrasonic transducer that frequency is 20kHz, the second cylinder reaction chamber
One end fixed frequency for closing on charging aperture be 28kHz high power altrasonic transducer, the second cylinder reaction chamber closes on
Material mouth one end is provided with the high power altrasonic transducer that frequency is 35kHz, one end for closing on charging aperture of the 3rd cylinder reaction chamber
Fixed frequency is 20kHz high power altrasonic transducer, and discharging opening one end of closing on of the second cylinder reaction chamber is provided with frequency
For 35kHz high power altrasonic transducer.
2. a kind of highly effective continuous frequency mixing ultrasonic breaking-wall cell processing unit according to claim 1, it is characterised in that institute
State breaking-wall cell ultrasonic reaction cavity and multiple cascades are carried out by discharging opening and charging aperture according to processing sample size and species.
3. a kind of highly effective continuous frequency mixing ultrasonic breaking-wall cell processing unit according to claim 1, it is characterised in that institute
State breaking-wall cell ultrasonic reaction cavity charging aperture and flow control valve is installed.
4. a kind of highly effective continuous frequency mixing ultrasonic breaking-wall cell processing unit according to claim 1, it is characterised in that enter
Material mouth is provided with discharging opening valve provided with charging aperture valve, discharging opening.
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| CN107303534A (en) * | 2016-04-19 | 2017-10-31 | 林荣良 | Combine refrigeration-type ultrasonic wave and disperse disintegrating apparatus |
| CN107557352A (en) * | 2017-09-14 | 2018-01-09 | 广东体必康生物科技有限公司 | The method of ultrasonic solid phase smudge cells and application |
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Effective date of registration: 20190924 Address after: Room 2518, Building No. 2, No. 38, Xiangfang District and Xinglu Road, Harbin, Heilongjiang Province, 150000 Patentee after: Harbin Youmai Leisheng Technology Co., Ltd. Address before: 710082 No. 81 labour Road, Lianhu District, Shaanxi, Xi'an Patentee before: Yang Hao |