CN104939843B - Implanted cancer relapse real-time monitoring system - Google Patents
Implanted cancer relapse real-time monitoring system Download PDFInfo
- Publication number
- CN104939843B CN104939843B CN201510406415.3A CN201510406415A CN104939843B CN 104939843 B CN104939843 B CN 104939843B CN 201510406415 A CN201510406415 A CN 201510406415A CN 104939843 B CN104939843 B CN 104939843B
- Authority
- CN
- China
- Prior art keywords
- circulation
- free rna
- monitoring system
- free
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 124
- 201000011510 cancer Diseases 0.000 title claims abstract description 113
- 238000012544 monitoring process Methods 0.000 title claims abstract description 78
- 230000004087 circulation Effects 0.000 claims description 196
- 238000004891 communication Methods 0.000 claims description 50
- 230000005611 electricity Effects 0.000 claims description 28
- 238000006243 chemical reaction Methods 0.000 claims description 26
- 238000009396 hybridization Methods 0.000 claims description 20
- 239000000523 sample Substances 0.000 claims description 20
- 230000001939 inductive effect Effects 0.000 claims description 19
- 238000012545 processing Methods 0.000 claims description 18
- 108091093037 Peptide nucleic acid Proteins 0.000 claims description 16
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 11
- 230000005059 dormancy Effects 0.000 claims description 11
- 239000002070 nanowire Substances 0.000 claims description 11
- 229910052710 silicon Inorganic materials 0.000 claims description 11
- 239000010703 silicon Substances 0.000 claims description 11
- 230000000284 resting effect Effects 0.000 claims description 8
- 230000008859 change Effects 0.000 claims description 6
- 230000009870 specific binding Effects 0.000 claims description 2
- 238000003491 array Methods 0.000 claims 1
- 239000012530 fluid Substances 0.000 abstract description 15
- 150000007523 nucleic acids Chemical class 0.000 abstract description 9
- 108020004707 nucleic acids Proteins 0.000 abstract description 8
- 102000039446 nucleic acids Human genes 0.000 abstract description 8
- 238000000338 in vitro Methods 0.000 abstract description 6
- 238000012546 transfer Methods 0.000 abstract description 5
- 230000005540 biological transmission Effects 0.000 abstract description 4
- 238000002513 implantation Methods 0.000 abstract description 3
- 239000003147 molecular marker Substances 0.000 abstract description 2
- 238000001727 in vivo Methods 0.000 abstract 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 192
- 108020004999 messenger RNA Proteins 0.000 description 15
- 210000001519 tissue Anatomy 0.000 description 14
- 238000001514 detection method Methods 0.000 description 12
- 238000005516 engineering process Methods 0.000 description 9
- 210000002381 plasma Anatomy 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 238000012986 modification Methods 0.000 description 7
- 230000004048 modification Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 108091070501 miRNA Proteins 0.000 description 6
- 230000033228 biological regulation Effects 0.000 description 5
- 239000002679 microRNA Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 238000001338 self-assembly Methods 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 238000006884 silylation reaction Methods 0.000 description 5
- 206010006187 Breast cancer Diseases 0.000 description 4
- 208000026310 Breast neoplasm Diseases 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 102000003910 Cyclin D Human genes 0.000 description 3
- 108090000259 Cyclin D Proteins 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 230000002980 postoperative effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000004065 semiconductor Substances 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 210000000707 wrist Anatomy 0.000 description 3
- 108050006400 Cyclin Proteins 0.000 description 2
- 102000016736 Cyclin Human genes 0.000 description 2
- 206010027476 Metastases Diseases 0.000 description 2
- 108020005196 Mitochondrial DNA Proteins 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000004020 conductor Substances 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 230000009401 metastasis Effects 0.000 description 2
- 238000004393 prognosis Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 208000028782 Hereditary disease Diseases 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 208000024556 Mendelian disease Diseases 0.000 description 1
- 108091033773 MiR-155 Proteins 0.000 description 1
- 108700011259 MicroRNAs Proteins 0.000 description 1
- 108091028049 Mir-221 microRNA Proteins 0.000 description 1
- 208000002454 Nasopharyngeal Carcinoma Diseases 0.000 description 1
- 206010061306 Nasopharyngeal cancer Diseases 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 102000004245 Proteasome Endopeptidase Complex Human genes 0.000 description 1
- 108090000708 Proteasome Endopeptidase Complex Proteins 0.000 description 1
- 102000052575 Proto-Oncogene Human genes 0.000 description 1
- 108700020978 Proto-Oncogene Proteins 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 102000044209 Tumor Suppressor Genes Human genes 0.000 description 1
- 108700025716 Tumor Suppressor Genes Proteins 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000001640 apoptogenic effect Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 201000008275 breast carcinoma Diseases 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 102000015694 estrogen receptors Human genes 0.000 description 1
- 108010038795 estrogen receptors Proteins 0.000 description 1
- 201000005787 hematologic cancer Diseases 0.000 description 1
- 208000024200 hematopoietic and lymphoid system neoplasm Diseases 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 108091064399 miR-10b stem-loop Proteins 0.000 description 1
- 108091091360 miR-125b stem-loop Proteins 0.000 description 1
- 108091058688 miR-141 stem-loop Proteins 0.000 description 1
- 108091045665 miR-202 stem-loop Proteins 0.000 description 1
- 108091062762 miR-21 stem-loop Proteins 0.000 description 1
- 108091041631 miR-21-1 stem-loop Proteins 0.000 description 1
- 108091044442 miR-21-2 stem-loop Proteins 0.000 description 1
- 108091061917 miR-221 stem-loop Proteins 0.000 description 1
- 108091063489 miR-221-1 stem-loop Proteins 0.000 description 1
- 108091055391 miR-221-2 stem-loop Proteins 0.000 description 1
- 108091031076 miR-221-3 stem-loop Proteins 0.000 description 1
- 108091050195 miR-302b stem-loop Proteins 0.000 description 1
- 108091029119 miR-34a stem-loop Proteins 0.000 description 1
- 108091090987 miR-425 stem-loop Proteins 0.000 description 1
- 108091034121 miR-92a stem-loop Proteins 0.000 description 1
- 108091041519 miR-92a-3 stem-loop Proteins 0.000 description 1
- -1 mitDNA) Proteins 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 201000011216 nasopharynx carcinoma Diseases 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 238000010837 poor prognosis Methods 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000012772 sequence design Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
- A61B5/1468—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using chemical or electrochemical methods, e.g. by polarographic means
- A61B5/1473—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using chemical or electrochemical methods, e.g. by polarographic means invasive, e.g. introduced into the body by a catheter
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/68—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
- A61B5/6801—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be attached to or worn on the body surface
- A61B5/6813—Specially adapted to be attached to a specific body part
- A61B5/6824—Arm or wrist
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/74—Details of notification to user or communication with user or patient ; user input means
- A61B5/742—Details of notification to user or communication with user or patient ; user input means using visual displays
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/74—Details of notification to user or communication with user or patient ; user input means
- A61B5/7455—Details of notification to user or communication with user or patient ; user input means characterised by tactile indication, e.g. vibration or electrical stimulation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/74—Details of notification to user or communication with user or patient ; user input means
- A61B5/746—Alarms related to a physiological condition, e.g. details of setting alarm thresholds or avoiding false alarms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Medical Informatics (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Molecular Biology (AREA)
- Surgery (AREA)
- Optics & Photonics (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physiology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses an implanted cancer relapse real-time monitoring system. The monitoring system comprises an in-vivo implantation assembly and an in-vitro assembly, wherein the in-vivo implantation assembly can convert concentration information of circulating cell-free nucleic acids in a tissue fluid into transmittal digital information and transmit the digital information to the in-vitro assembly in a wireless transmission manner; the in-vitro assembly analyzes the received circulating cell-free nucleic acid information and informs a user of the implanted cancer relapse real-time monitoring system of paying attention to cancer relapse danger in an information display or warning manner. The implanted cancer relapse real-time monitoring system can monitor a molecular marker, circulating cell-free nucleic acids, of cancer relapse/transfer in real time and can detect the cancer relapse danger in an early stage of cancer relapse, so that a patient suffering from cancer relapse can be treated in time.
Description
Technical field
The invention belongs to medical instruments field, it is related to a kind of cancer return monitoring system, and in particular to a kind of implanted cancer
Disease recurs real-time monitoring system.
Background technology
The curative competence of current China cancer is also not fully up to expectations, and about 1/3 can be controlled in whole cancer patients
More, the chance of long term survival is obtained, but there are relapse and metastasis in the course of disease in the cancer patient for also having half or so.Cancer return is
The cancer that finger is brought under control occurs on primary organ again, or cancer invades lymph vessels or body cavity from original site, moves
Elsewhere continued growth is moved on to, is formed and the same type of tumour of primary tumor.Some cancers can be in some months after treating first
Or recurred after several years.Therefore for cancer patient, the monitoring for carrying out postoperative recurrence transfer is very important.Cancer return
It is more early that the sign of transfer finds, it becomes possible to takes counter-measure in time, the effective possibility for suppressing cancer return transfer, prolongs
Cancer patient long postoperative life cycle.
The free RNA (circulating cell-free nucleic acids) of circulation refers to be present in human circulation system
In system, extracellular micro endogenous or Exogenous Nucleic Acid fragment, including core DNA, mitochondrial DNA are free on
(mitochondrial DNA, mitDNA), viral DNA, mRNA (messenger RNA, mRNA) and Microrna
(microRNA, the free RNA of circulation) etc..MRNA stability in itself is relatively low, while the RNase level liter in tumor patient blood
Height, therefore previously think that circulation free mRNA degradation speed is very fast, clinical value is limited.There is research to be disclosed in tumor patient
Serum in, RNA exists with fat egg from the form of complex.It is another studies have found that, free mRNA multi-source is deposited in Apoptosis
In being apoptotic body, therefore free mRNA stability in tumor patient blood is higher.These features all examine free mRNA
Survey is applied to clinic becomes possibility.Research discovery, can detect cyclin D 1mRNA in the blood plasma of patient with breast cancer
Patient the overall survival patient negative significantly lower than blood plasma cyclin D1mRNA；Prognosis is pointed out in clinical pathologic characteristic
In preferable patient, patient's prognosis that cyclin D 1mRNA are detected in blood plasma is poor；In the patient of his silent former times fragrant treatment, blood
The patient of the overall survival significantly lower than cyclin D1mRNA feminine genders of slurry cyclin D 1mRNA positive patient.Additionally, grinding
Study carefully discovery plasma of patients with nasopharyngeal carcinoma mRNA integrality reductions, plasma free mRNA integralities substantially rise after radiotherapy, the trip of rising
Can be used as the index of disease amelioration from mRNA level in-site.The studies above achievement shows that the mRNA dissociated in blood can turn into cancer morning
Phase diagnosis, the molecular marker of relapse and metastasis monitoring.
MiRNA plays an important roll in gene expression, post-transcriptional control, and may play proto-oncogene or tumor suppressor gene
Effect.They have high stability in plasma or serum, and the generation, development with tumour are closely related, and these speciality make
Free RNA must be circulated as a kind of potential tumor markers.It is found that circulation trip in kinds of tumors blood samples of patients at present
From the abnormal change of rna level.MiR-155, miR-202, miR-425, miR-302b and miR-125b etc. are to report in recent years
The expression in healthy population and plasma of breast cancer patients or serum have the free RNA of the circulation of notable difference, in the morning of breast cancer
There is potential value in phase examination；Additionally, levels of the miR-115 in hormone-sensitive and the insensitive blood serum of patients with human breast carcinoma of hormone
Variant, miR-10b levels in the patients serum of estrogen receptor negative are raised.For colorectal cancer patients, miR-92a is in morning
Sensitiveness higher has been embodied in phase detection；The rising of miR-21, the reduction of miR34a also have diagnosis effect higher；
MiR-141 and miR-221 are then considered as related to poor prognosis.
The mode of current patient monitoring cancer return is mainly periodically to be checked to hospital, this mode for patient,
One is inconvenient, expends the time, and two is to check not in time, it is possible to miss the possibility of early detection cancer return.For side
Just cancer patient can timely check oneself the recurrence of cancer, study a kind of equipment that can be recurred with real-time monitoring cancer patient
It is necessary.
The content of the invention
In order to solve the deficiencies in the prior art, it is an object of the invention to provide a kind of implanted cancer return real-time monitoring
System, the monitoring system is by monitoring monitoring of the level completion of the free RNA of circulation in hypodermis liquid to cancer return.Institute
State monitoring system and one threshold value of the free RNA of circulation is set, when the level of the free RNA of the circulation for detecting is up to or over this
During threshold value, the monitoring system can then notify that monitoring system wearer cancer return may have been sent out by way of sending alarm
Raw, cancer patient can enable the state of an illness to be controlled early to be made a definite diagnosis to hospital in time.
To achieve these goals, this invention takes following technical scheme：
The invention provides a kind of implanted cancer return real-time monitoring system, the cancer return real-time monitoring system bag
Include et al. Ke component 1 and external component 2；The et al. Ke component 1 includes the free RNA sensors 11, data processing of circulation
Module 12, the first wireless communication module 13；The data processing module 12 includes input interface 121, microprocessor 122, output
Interface 123, reactor 124；The input interface 121 receives the electrochemical signals of the free output of RNA sensors 11 of the circulation
And it is converted into the microprocessor 122 is input into after data signal, the microprocessor 122 carries out agreement to data signal and turns
The external component 2 is uploaded to via first wireless communication module 13 by the output interface 123 after changing；It is described external
Component 2 include data receiver 21, the data receiver 21 include controller 211, display 212, siren 213,
Second wireless communication module 214；The controller 211 receives and analyzes the free RNA of circulation that the et al. Ke component 1 is uploaded
Signal, will show, the analysis program of the controller 211 sets afterwards in the free RNA information transmissions of circulation to the display 212
A threshold value is put, when the level of the free RNA of circulation exceedes the threshold value, the controller 211 issues instructions to the siren
213, the siren 213 sends alarm, notifies that wearer notes the danger of cancer return, second wireless communication module
On 214 reactors 124 for passing in the et al. Ke component 1 control command of the controller 211, the reaction
Device 124 controls unlatching or the resting state of the free RNA sensors 11 of the circulation, when the free RNA sensors 11 of the circulation are stopped
During dormancy, the free RNA sensors 11 of circulation carry negative electricity, and RNA free with the circulation with negative electricity does not occur association reaction, when described
When the free RNA sensors 11 of circulation are opened, the free RNA sensors 11 of the circulation are not charged, free with the circulation with negative electricity
There is association reaction in RNA, the free RNA of electronegative circulation causes the electrical conductivity of the free RNA sensors 11 of the circulation to become
Change, so as to the concentration information that will circulate free RNA is converted into electrochemical signals.
Further, the external component 2 also includes intelligent mobile terminal equipment 22, and the data receiver 21 also includes
3rd wireless communication module 215, the controller 211 will circulate free RNA information and pass through the 3rd wireless communication module 215
It is sent in the intelligent mobile terminal equipment 22.
The intelligent mobile terminal equipment 22 can be smart mobile phone, flat board or computer.
Further, the siren 213 is vibrator, notifies that wearer notes the danger of cancer return by way of vibration
Danger.
Further, the siren 213 is photophore, notifies that wearer notes the danger of cancer return by way of luminous
Danger.
Further, the free RNA sensors 11 of the circulation include the free RNA trapping layer 111, hybridization signal inductive layer of circulation
112.The free RNA trapping layer 111 of circulation is sticked on the hybridization signal inductive layer 112.The free RNA captures of circulation
Layer 111 is made up of the capture probe specifically bound with the free RNA of circulation.
The capture probe that the RNA free with circulation that the present invention is used specifically binds can be DNA, RNA, peptide nucleic acid or
Lock nucleic acid, as long as the probe being capable of RNA specific bindings free with circulation.RNA free for the circulation of known array comes
Say, those skilled in the art are routine techniques according to the capture probe that the free RNA sequence design of circulation hybridizes therewith.Preferably,
The capture probe is peptide nucleic acid (PNA).
PNA is the nucleic acid analog that a class replaces ribose (or deoxyribose) phosphate backbone with many phthalein amine chains, with structure
Stabilization, not by nuclease and proteasome degradation, can with base complementrity mode and single nucleic acid strands, cytotoxicity low, electroneutral
The features such as.PNA has stronger adhesion with DNA, RNA, there is good specific and higher sensitivity, and due to it
The property of electroneutral causes PNA-DNA, and the double-strand of PNA-RNA is more stable, and with corresponding DNA-DNA, DNA-RNA double-strands are compared,
With melting temperature higher.Due to the structural stability of PNA so that it can in various chemical reagent stable existence, can
Neutralized within the scope of temperature wider with being stably present in sour environment.Therefore, PNA can be widely applied to pathogen, hereditary disease
In the molecule hybridization of detection, in situ hybridization, mutation analysis, anticancer, antiviral antisense nucleic acids research and application.
Further, the hybridization signal inductive layer 112 is included by silicon nanowire array.The hybridization signal inductive layer 112
It is on using the silicon nanowires of conventional semiconductors technology processing, by silylation modification, surface unimolecule film layer self assembly work
Sequence is prepared from, and being fixed by capture probe can form the free RNA sensors of described circulation.The free RNA sensors of circulation
Preparation process be well known to those skilled in the art.
Further, first wireless communication module 13, second wireless communication module 214, the 3rd channel radio
Letter module 215 is using any one in Bluetooth protocol or Zigbee protocol.
Further, the data receiver 12 can be worn at any part on cancer patient's body, preferably wear
In wrist.In addition, the data receiver 12 can not also be worn on a certain position of the body of cancer patient, but make
For in vitro accessory is used.
Further, the control of unlatching or the dormancy to the free RNA sensors of circulation is realized in the timing of the controller 211.Control
The interval time that system order is assigned continuously adjustabe between 1s to 1800s, regulation stepping is 2s.
The species that can be used for the free RNA of circulation of the invention is that those have significant correlation between cancer to be detected
The free RNA of circulation, i.e., by detecting that the concentration of the free RNA of circulation is that can determine whether whether cancer recurs.The free RNA bags of circulation
Include circulation free mRNA, the free miRNA of circulation.
Preferably, it is the free miRNA of circulation to circulate free RNA.
The application method of implanted cancer return real-time monitoring system of the invention：
First, the implanted cancer return real-time monitoring system is controlled in a dormant state by the controller 211,
Free RNA sensors 11 are now circulated with negative electricity (institute is electrically charged atomic weak, will not be damaged to human body), by et al. Ke
Component implantation is subcutaneous；
Secondly, the implanted cancer return real-time monitoring system is opened by the controller 211, circulation is dissociated
RNA sensors 11 are not charged, and the free RNA of the now circulation in tissue fluid can be combined with the free RNA sensors 11 of circulation；
Finally, after reaction certain hour, closing implanted cancer return real-time monitoring system by the controller 211 makes
Be again at resting state, now circulate free RNA sensors 11 and carry negative electricity, RNA free with circulation is sensed in tissue fluid
The free RNA of circulation that device 11 is combined is separated with the free RNA sensors 11 of circulation, the implanted cancer return real-time monitoring system
For detection next time is ready.
Cancer patient is known by observing the reaction for circulating free RNA information and siren 213 on display 212
Whether cancer recurs.
The prior art indicate that the free RNA of the circulation dissociated in blood can be used as the potential source biomolecule for detecting all kinds of cancers and disease
Label, for the detection of the early detection and cancer return of patient's peripheral blood cancer.Tissue fluid is blood plasma from capillary wall
Filter and formed, in addition to without macro-molecular protein, other compositions are substantially identical with blood plasma.Therefore dissociate present in blood
The free RNA of circulation is equally existed in tissue fluid, therefore by detecting that the level of the free free RNA of circulation in tissue fluid can use
Early detection and the detection of cancer return in cancer, act on the same with blood.
The advantages of the present invention：
1st, implanted cancer return real-time monitoring system of the invention is dissociated rna level by detecting the circulation in tissue fluid
Whether recurred to judge that cancer patient is postoperative, the diagnosis of molecular level be able to can find in recurrence early stage, be it is effective and
When take corresponding treatment means provide diagnostic base.
2nd, implanted cancer return real-time monitoring system of the invention can be realized monitoring anywhere or anytime, reach dynamic in real time
The purpose of state monitoring, can make cancer patient easily timely grasp the current intelligence of cancer return transfer.
3rd, implanted cancer return real-time monitoring system of the invention is a monitoring system that can be reused, the prison
Examining system realizes the unlatching of monitoring system or cutting for resting state by controlling the charging property of the free RNA sensors of circulation
Change, it is to avoid often detect once needs the operation for being implanted into monitoring system again afterwards.
Brief description of the drawings
Fig. 1 shows a kind of structural representation of implanted cancer return real-time monitoring system of the invention；
A kind of structural representation of Fig. 2 display display implanted cancer return real-time monitoring systems of the invention；
Fig. 3 shows the structural representation of the free RNA sensors of circulation of the invention；
Wherein, 1：Et al. Ke component；11：The free RNA sensors of circulation；111：The free RNA trapping layer of circulation；112：It is miscellaneous
Hand over induction of signal layer；12：Data processing module；121：Input interface；122：Microprocessor；123：Output interface；124：Reaction
Device；13：First wireless communication module；2：External component；21：Data receiver；211：Controller；212：Display；213：
Siren；214：Second wireless communication module；215：3rd wireless communication module；22：Intelligent mobile terminal equipment.
Specific embodiment
Below in conjunction with specific embodiment, the present invention is furture elucidated.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limitation the scope of the present invention.
A kind of implanted cancer return real-time monitoring system of embodiment 1
A kind of implanted cancer return real-time monitoring system, cancer return real-time monitoring system includes et al. Ke component 1
With external component 2；Et al. Ke component 1 includes the free RNA sensors 11, data processing module 12 of circulation, the first radio communication
Module 13；Data processing module 12 includes input interface 121, microprocessor 122, output interface 123；Reactor 124；Input connects
Mouthfuls 121 electrochemical signals for receiving the free output of RNA sensors 11 of circulation and by it be converted into data signal after be input into microprocessor
Device 122, microprocessor 122 passes through output interface 123 via the first wireless communication module after protocol conversion is carried out to data signal
13 are uploaded to external component 2；External component 2 includes data receiver 21, and data receiver 21 includes controller 211, shows
Show device 212, siren 213, the second wireless communication module 214；Controller 211 is received and analyzes the upload of et al. Ke component 1
The free RNA signals of circulation, will show, the analysis journey of controller 211 in the free RNA information transmissions of circulation to display 212 afterwards
Sequence sets a threshold value, and when the level of the free RNA of circulation exceedes the threshold value, controller 211 issues instructions to the siren
213, siren 213 sends alarm, notifies that wearer notes the danger of cancer return, and the second wireless communication module 214 will control
On the reactor 124 that the control command of device 211 is passed in et al. Ke component 1, the free RNA sensings of the control circulation of reactor 124
The unlatching of device 11 or resting state, when free RNA 11 dormancy of sensor of circulation, the free RNA sensors of circulation carry negative electricity, with
There is no association reaction in the free RNA of the circulation with negative electricity, when the free RNA sensors 11 of circulation are opened, the free RNA of circulation is passed
Sensor 11 is not charged, and RNA free with the circulation with negative electricity occurs association reaction, and the free RNA of electronegative circulation causes circulation
The electrical conductivity of free RNA sensors 11 changes, so as to the concentration information that will circulate free RNA is converted into electrochemical signals.
Siren 213 is vibrator, notifies that wearer notes the danger of cancer return by way of vibration.
The free RNA sensors 11 of circulation include the free RNA trapping layer 111, hybridization signal inductive layer 112 of circulation.Institute
Stating the free RNA trapping layer 111 of circulation is made up of the capture probe specifically bound with the free RNA of circulation.Capture probe is peptide
Nucleic acid (PNA).
Hybridization signal inductive layer 112 includes silicon nanowire array.Hybridization signal inductive layer 112 is to utilize conventional semiconductors
On the silicon nanowires of technology processing, it is prepared from by silylation modification, surface unimolecule film layer self assembly operation, by capture
Probe is fixed can form the free RNA sensors 11 of circulation.The preparation process of the free RNA sensors 11 of circulation is art technology
Known to personnel.
First wireless communication module 13, the second wireless communication module 214 use Bluetooth protocol.
Data receiver 12 can be worn at any part on cancer patient's body, for example, be worn in wrist.
The control of unlatching or the dormancy to the free RNA sensors of circulation is realized in the timing of controller 211.What control command was assigned
Interval time continuously adjustabe between 1s to 1800s, regulation stepping is 2s.
The free RNA of circulation in the present embodiment refers to miRNA.
The application method of above-mentioned implanted cancer return real-time monitoring system：
1st, implanted cancer return real-time monitoring system is controlled in a dormant state by controller 211, now circulation trip
From RNA sensors 11 with negative electricity (institute is electrically charged atomic weak, will not be damaged to human body), et al. Ke component is implanted into skin
Under；
2nd, implanted cancer return real-time monitoring system is opened by controller 211, makes the free RNA sensors 11 of circulation not
Powered, the free RNA of circulation now in tissue fluid can be combined with the free RNA sensors 11 of circulation；
3rd, after reaction certain hour, implanted cancer return real-time monitoring system is closed by controller 211 and is allowed to again
In a dormant state, free RNA sensors 11 are now circulated and carries negative electricity, with the free knot of RNA sensors 11 of circulation in tissue fluid
The free RNA of the circulation of conjunction is separated with the free RNA sensors 11 of circulation, and implanted cancer return real-time monitoring system is next time
Detection is ready.
4th, by observing whether the free RNA information of the circulation shown on display 212 and siren are vibrated, cancer patient
Know whether cancer recurs.
A kind of implanted cancer return real-time monitoring system of embodiment 2
A kind of implanted cancer return real-time monitoring system, cancer return real-time monitoring system includes et al. Ke component 1
With external component 2；Et al. Ke component 1 includes the free RNA sensors 11, data processing module 12 of circulation, the first radio communication
Module 13；Data processing module 12 includes input interface 121, microprocessor 122, output interface 123；Reactor 124；Input connects
Mouthfuls 121 electrochemical signals for receiving the free output of RNA sensors 11 of circulation and by it be converted into data signal after be input into microprocessor
Device 122, microprocessor 122 passes through output interface 123 via the first wireless communication module after protocol conversion is carried out to data signal
13 are uploaded to external component 2；External component 2 includes data receiver 21, and data receiver 21 includes controller 211, shows
Show device 212, siren 213, the second wireless communication module 214；Controller 211 is received and analyzes the upload of et al. Ke component 1
The free RNA signals of circulation, will show, the analysis journey of controller 211 in the free RNA information transmissions of circulation to display 212 afterwards
Sequence sets a threshold value, and when the level of the free RNA of circulation exceedes the threshold value, controller 211 issues instructions to the siren
213, siren 213 sends alarm, notifies that wearer notes the danger of cancer return, and the second wireless communication module 214 will control
On the reactor 124 that the control command of device 211 is passed in et al. Ke component 1, the free RNA sensings of the control circulation of reactor 124
The unlatching of device 11 or resting state, when free RNA 11 dormancy of sensor of circulation, the free RNA sensors 11 of circulation carry negative electricity,
There is no association reaction in RNA free with the circulation with negative electricity, when the free RNA sensors 11 of circulation are opened, the free RNA of circulation
Sensor 11 is not charged, and RNA free with the circulation with negative electricity occurs association reaction, and the free RNA of electronegative circulation causes to follow
The electrical conductivity toured around from RNA sensors 11 changes, so as to the concentration information that will circulate free RNA is converted into electrochemistry letter
Number.
Siren 213 is photophore, notifies that wearer notes the danger of cancer return by way of luminous.
The free RNA sensors 11 of circulation include the free RNA trapping layer 111, hybridization signal inductive layer 112 of circulation.Institute
Stating the free RNA trapping layer 111 of circulation is made up of the capture probe specifically bound with the free RNA of circulation.Capture probe is peptide
Nucleic acid (PNA).
Hybridization signal inductive layer 112 is constituted including silicon nanowire array.Hybridization signal inductive layer 112 is using tradition half
On the silicon nanowires of conductor technology processing, it is prepared from by silylation modification, surface unimolecule film layer self assembly operation, is passed through
Capture probe is fixed can form the free RNA sensors 11 of circulation.The preparation process of the free RNA sensors 11 of circulation is this area
Known to technical staff.
First wireless communication module 13, the second wireless communication module 214 use Zigbee protocol.
Data receiver 12 is used as in vitro accessory.
The control of unlatching or the dormancy to the free RNA sensors 11 of circulation is realized in the timing of controller 211.Control command is assigned
Interval time between 1s to 1800s continuously adjustabe, regulation stepping be 2s.
The free RNA of circulation in the present embodiment refers to circulation free mRNA.
The application method of above-mentioned implanted cancer return real-time monitoring system：
1st, implanted cancer return real-time monitoring system is controlled in a dormant state by controller 211, now circulation trip
From RNA sensors 11 with negative electricity (institute is electrically charged atomic weak, will not be damaged to human body), et al. Ke component is implanted into skin
Under；
2nd, implanted cancer return real-time monitoring system is opened by controller 211, makes the free RNA sensors 11 of circulation not
Powered, the free RNA of circulation now in tissue fluid can be combined with the free RNA sensors 11 of circulation；
3rd, after reaction certain hour, implanted cancer return real-time monitoring system is closed by controller 211 and is allowed to again
In a dormant state, free RNA sensors 11 are now circulated and carries negative electricity, with the free knot of RNA sensors 11 of circulation in tissue fluid
The free RNA of the circulation of conjunction is separated with the free RNA sensors 11 of circulation, and implanted cancer return real-time monitoring system is next time
Detection is ready.
4th, by observing whether the free RNA information of the circulation shown on display 212 and siren light, cancer patient
Know whether cancer recurs.
A kind of implanted cancer return real-time monitoring system of embodiment 3
A kind of implanted cancer return real-time monitoring system, cancer return real-time monitoring system includes et al. Ke component 1
With external component 2；Et al. Ke component 1 includes the free RNA sensors 11, data processing module 12 of circulation, the first radio communication
Module 13；Data processing module 12 includes input interface 121, microprocessor 122, output interface 123；Reactor 124；Input connects
Mouthfuls 121 electrochemical signals for receiving the free output of RNA sensors 11 of circulation and by it be converted into data signal after be input into microprocessor
Device 122, microprocessor 122 passes through output interface 123 via the first wireless communication module after protocol conversion is carried out to data signal
13 are uploaded to external component 2；External component 2 includes data receiver 21, intelligent mobile terminal equipment 22；Data receiver
21 include controller 211, display 212, siren 213, the second wireless communication module 214, the 3rd wireless communication module 215；
Controller 211 is received and analyzes the free RNA signals of circulation of the upload of et al. Ke component 1, afterwards passes the free RNA information of circulation
Display on display 212 is sent to, the analysis program of controller 211 sets a threshold value, is somebody's turn to do when the level of the free RNA of circulation exceedes
During threshold value, controller 211 issues instructions to the siren 213, and siren 213 sends alarm, notifies that wearer notes cancer
The danger of recurrence, the reaction that the second wireless communication module 214 passes in et al. Ke component 1 control command of controller 211
On device 124, the control of reactor 124 circulates unlatching or the resting state of free RNA sensors 11, when the free RNA sensors of circulation
During 11 dormancy, the free RNA sensors of circulation carry negative electricity, and RNA free with the circulation with negative electricity does not occur association reaction, when following
Tour around from RNA sensors 11 open when, the free RNA sensors of circulation are not charged, and RNA free with the circulation with negative electricity is tied
Reaction is closed, the electronegative free RNA of circulation causes the electrical conductivity for circulating free RNA sensors to change, so as to circulation be swum
Concentration information from RNA is converted into electrochemical signals.
Controller 211 will circulate free RNA information and be sent to intelligent mobile terminal by the 3rd wireless communication module 215 and set
On standby 22.The free RNA information of 22 pairs of circulations of intelligent mobile terminal equipment carries out remote storage and analysis.
Intelligent terminal 22 can be smart mobile phone, flat board or computer.
Data receiver 12 can be worn on any part of cancer patient's body, for example wrist.
First wireless communication module 13, the second wireless communication module 214, the 3rd wireless communication module 215 are assisted using bluetooth
View.
Siren 213 is vibrator, notifies that wearer notes the danger of cancer return by way of vibration.
The free RNA sensors 11 of circulation include the free RNA trapping layer 111, hybridization signal inductive layer 112 of circulation.Circulation trip
From RNA trapping layer 111 it is made up of the capture probe specifically bound with the free RNA of circulation.Capture probe is peptide nucleic acid
(PNA)。
Hybridization signal inductive layer 112 is constituted including silicon nanowire array.Hybridization signal inductive layer 112 is using tradition half
On the silicon nanowires of conductor technology processing, it is prepared from by silylation modification, surface unimolecule film layer self assembly operation, is passed through
Capture probe is fixed can form the free RNA sensors of circulation.The preparation process of the free RNA sensors of circulation is art technology
Known to personnel.
The control of unlatching or the dormancy to the free RNA sensors of circulation is realized in the timing of controller 211.What control command was assigned
Interval time continuously adjustabe between 1s to 1800s, regulation stepping is 2s.
The free RNA of circulation in the present embodiment refers to circulating tumor miRNA.
The application method of above-mentioned implanted cancer return real-time monitoring system：
1st, implanted cancer return real-time monitoring system is controlled in a dormant state by controller 211, now circulation trip
From RNA sensors 11 with negative electricity (institute is electrically charged atomic weak, will not be damaged to human body), et al. Ke component is implanted into skin
Under；
2nd, implanted cancer return real-time monitoring system is opened by controller 211, makes the free RNA sensors 11 of circulation not
Powered, the free RNA of circulation now in tissue fluid can be combined with the free RNA sensors 11 of circulation；
3rd, after reaction certain hour, implanted cancer return real-time monitoring system is closed by controller 211 and is allowed to again
In a dormant state, free RNA sensors 11 are now circulated and carries negative electricity, with the free knot of RNA sensors 11 of circulation in tissue fluid
The free RNA of the circulation of conjunction is separated with the free RNA sensors 11 of circulation, and implanted cancer return real-time monitoring system is next time
Detection is ready.
4th, by observe the free RNA information of circulation or intelligent mobile terminal equipment 22 that are shown on display 212 and
Whether siren 213 vibrates, and cancer patient knows whether cancer recurs.
A kind of implanted cancer return real-time monitoring system of embodiment 4
A kind of implanted cancer return real-time monitoring system, cancer return real-time monitoring system includes et al. Ke component 1
With external component 2；Et al. Ke component 1 includes the free RNA sensors 11, data processing module 12 of circulation, the first radio communication
Module 13；Data processing module 12 includes input interface 121, microprocessor 122, output interface 123；Reactor 124；Input connects
Mouthfuls 121 electrochemical signals for receiving the free output of RNA sensors 11 of circulation and by it be converted into data signal after be input into microprocessor
Device 122, microprocessor 122 passes through output interface 123 via the first wireless communication module after protocol conversion is carried out to data signal
13 are uploaded to external component 2；External component 2 includes data receiver 21, intelligent mobile terminal equipment 22；Data receiver
21 include controller 211, display 212, siren 213, the second wireless communication module 214, the 3rd wireless communication module 215；
Controller 211 is received and analyzes the free RNA signals of circulation of the upload of et al. Ke component 1, afterwards passes the free RNA information of circulation
It is sent on display and shows, the analysis program of controller 211 sets a threshold value, when the level of the free RNA of circulation exceedes the threshold
During value, controller 211 issues instructions to the siren 213, and siren 213 sends alarm, notifies that wearer notices that cancer is answered
The danger of hair, the reactor that the second wireless communication module 214 passes in et al. Ke component 1 control command of controller 211
On 124, the control of reactor 124 circulates unlatching or the resting state of free RNA sensors 11, when the free RNA sensors 11 of circulation
During dormancy, the free RNA sensors of circulation carry negative electricity, and RNA free with the circulation with negative electricity does not occur association reaction, works as circulation
When free RNA sensors 11 are opened, the free RNA sensors of circulation are not charged, and RNA free with the circulation with negative electricity is combined
Reaction, the electronegative free RNA of circulation causes the electrical conductivity for circulating free RNA sensors to change, so as to circulation is free
The concentration information of RNA is converted into electrochemical signals.
Controller 211 will circulate free RNA information and be sent to intelligent mobile terminal by the 3rd wireless communication module 215 and set
On standby 22.The free RNA information of 22 pairs of circulations of intelligent mobile terminal equipment carries out remote storage and analysis.
Intelligent terminal 22 can be smart mobile phone, flat board or computer.
Data receiver 12 is used as in vitro accessory.
First wireless communication module 13, the second wireless communication module 214, the 3rd wireless communication module 215 use ZigBee
Agreement.
Siren 213 is photophore, notifies that wearer notes the danger of cancer return by way of luminous.
The free RNA sensors 11 of circulation include the free RNA trapping layer 111, hybridization signal inductive layer 112 of circulation.Circulation trip
From RNA trapping layer 111 it is made up of the capture probe specifically bound with the free RNA of circulation.Capture probe is peptide nucleic acid
(PNA)。
Hybridization signal inductive layer 112 includes silicon nanowire array.Hybridization signal inductive layer 112 is to utilize conventional semiconductors
On the silicon nanowires of technology processing, it is prepared from by silylation modification, surface unimolecule film layer self assembly operation, by capture
Probe is fixed can form the free RNA sensors 11 of circulation.The preparation process of the free RNA sensors 11 of circulation is art technology
Known to personnel.
The control of unlatching or the dormancy to the free RNA sensors 11 of circulation is realized in the timing of controller 211.Control command is assigned
Interval time between 1s to 1800s continuously adjustabe, regulation stepping be 2s.
The free RNA of circulation in the present embodiment refers to circulation free mRNA.
The application method of above-mentioned implanted cancer return real-time monitoring system：
1st, implanted cancer return real-time monitoring system is controlled in a dormant state by controller 211, now circulation trip
From RNA sensors 11 with negative electricity (institute is electrically charged atomic weak, will not be damaged to human body), et al. Ke component is implanted into skin
Under；
2nd, implanted cancer return real-time monitoring system is opened by controller 211, makes the free RNA sensors 11 of circulation not
Powered, the free RNA of circulation now in tissue fluid can be combined with the free RNA sensors 11 of circulation；
3rd, after reaction certain hour, implanted cancer return real-time monitoring system is closed by controller 211 and is allowed to again
In a dormant state, free RNA sensors 11 are now circulated and carries negative electricity, with the free knot of RNA sensors 11 of circulation in tissue fluid
The free RNA of the circulation of conjunction is separated with the free RNA sensors 11 of circulation, and implanted cancer return real-time monitoring system is next time
Detection is ready.
4th, by observe the free RNA information of circulation or intelligent mobile terminal equipment 22 that are shown on display 212 and
Whether siren 213 lights, and cancer patient knows whether cancer recurs.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that：Not
Can these embodiments be carried out with various changes, modification, replacement and modification in the case of departing from principle of the invention and objective, this
The scope of invention is limited by claim and its equivalent.
Claims (9)
1. a kind of implanted cancer return real-time monitoring system, it is characterised in that the monitoring system includes et al. Ke component
And external component (2) (1)；The et al. Ke component (1) includes circulation free RNA sensors (11), data processing module
(12), the first wireless communication module (13)；The data processing module (12) includes input interface (121), microprocessor
(122), output interface (123), reactor (124)；The input interface (121) receives the free RNA sensors of the circulation
(11) electrochemical signals of output and it is converted into after data signal it is input into the microprocessor (122), the microprocessor
(122) output interface (123) is passed through via first wireless communication module after protocol conversion is carried out to data signal
(13) it is uploaded to the external component (2)；The external component (2) includes data receiver (21), and the data receiver sets
Standby (21) include controller (211), display (212), siren (213), the second wireless communication module (214)；The control
Device (211) receives and analyzes the free RNA signals of circulation that the et al. Ke component (1) uploads, afterwards by the free RNA letters of circulation
Breath is sent on the display (212) and shows, the analysis program of the controller (211) sets a threshold value, when circulation is swum
When exceeding the threshold value from the level of RNA, the controller (211) issues instructions to the siren (213), the siren
(213) alarm is sent, notifies that wearer notes the danger of cancer return, second wireless communication module (214) is by the control
On the reactor (124) that the control command of device (211) processed is passed in the et al. Ke component (1), the circulation is controlled
The unlatching of free RNA sensors (11) or resting state, when free RNA sensor (11) dormancy of the circulation, circulation is free
There is no association reaction in RNA sensors (11), with negative electricity, RNA free with the circulation with negative electricity as the free RNA of the circulation
When sensor (11) is opened, free RNA sensors (11) is not charged for the circulation, and RNA free with the circulation with negative electricity occurs
Association reaction, the free RNA of electronegative circulation causes the electrical conductivity of free RNA sensors (11) of the circulation to change, from
And the concentration information that will circulate free RNA is converted into electrochemical signals.
2. monitoring system according to claim 1, it is characterised in that the external component (2) also includes intelligent mobile end
End equipment (22), the data receiver (21) also includes the 3rd wireless communication module (215), and the controller (211) will
The free RNA information of circulation is sent in the intelligent mobile terminal equipment (22) by the 3rd wireless communication module (215).
3. monitoring system according to claim 1 and 2, it is characterised in that the siren (213) is vibrator, is passed through
The mode of vibration notifies that wearer notes the danger of cancer return.
4. monitoring system according to claim 1 and 2, it is characterised in that the siren (213) is photophore, is passed through
Luminous mode notifies that wearer notes the danger of cancer return.
5. monitoring system according to claim 1 and 2, it is characterised in that free RNA sensors (11) of the circulation includes
Circulation free RNA trapping layer (111), hybridization signal inductive layer (112)；Free RNA trapping layer (111) of circulation sticks to institute
State on hybridization signal inductive layer (112).
6. monitoring system according to claim 5, it is characterised in that free RNA trapping layer (111) of the circulation be by with
The capture probe of the free RNA specific bindings of circulation is constituted.
7. monitoring system according to claim 6, it is characterised in that the capture probe is peptide nucleic acid.
8. monitoring system according to claim 5, it is characterised in that the hybridization signal inductive layer (112) is received including silicon
Nanowire arrays.
9. monitoring system according to claim 2, it is characterised in that first wireless communication module (13), described
Two wireless communication modules (214), the 3rd wireless communication module (215) are using any in Bluetooth protocol or Zigbee protocol
It is a kind of.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510406415.3A CN104939843B (en) | 2015-07-12 | 2015-07-12 | Implanted cancer relapse real-time monitoring system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510406415.3A CN104939843B (en) | 2015-07-12 | 2015-07-12 | Implanted cancer relapse real-time monitoring system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104939843A CN104939843A (en) | 2015-09-30 |
| CN104939843B true CN104939843B (en) | 2017-05-17 |
Family
ID=54155323
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510406415.3A Active CN104939843B (en) | 2015-07-12 | 2015-07-12 | Implanted cancer relapse real-time monitoring system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104939843B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2018331306A1 (en) * | 2017-09-13 | 2020-03-05 | Hologic, Inc. | Wireless active monitoring implant system |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869468A (en) * | 2010-06-21 | 2010-10-27 | 黄爱强 | Monitoring system for human tumor recurrence and metastasis |
| CN103932680A (en) * | 2014-04-15 | 2014-07-23 | 广州玖玖伍捌信息科技有限公司 | Human body tumor recurrence monitoring and thermal therapy integrated system |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8246565B2 (en) * | 2009-02-25 | 2012-08-21 | The Invention Science Fund I, Llc | Device for passively removing a target component from blood or lymph of a vertebrate subject |
| WO2013010106A2 (en) * | 2011-07-13 | 2013-01-17 | Lockheed Martin Corporation | Three dimensional microfluidic multiplexed diagnostic system |
| US20130158378A1 (en) * | 2011-09-22 | 2013-06-20 | The Ohio State University | Ionic barrier for floating gate in vivo biosensors |
-
2015
- 2015-07-12 CN CN201510406415.3A patent/CN104939843B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869468A (en) * | 2010-06-21 | 2010-10-27 | 黄爱强 | Monitoring system for human tumor recurrence and metastasis |
| CN103932680A (en) * | 2014-04-15 | 2014-07-23 | 广州玖玖伍捌信息科技有限公司 | Human body tumor recurrence monitoring and thermal therapy integrated system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104939843A (en) | 2015-09-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hao et al. | Graphene-based fully integrated portable nanosensing system for on-line detection of cytokine biomarkers in saliva | |
| Mortellaro et al. | Performance characterization of an abiotic and fluorescent-based continuous glucose monitoring system in patients with type 1 diabetes | |
| Wang et al. | Real-time, label-free monitoring of the cell cycle with a cellular impedance sensing chip | |
| CN109655512A (en) | A kind of excretion body detecting method based on aptamers and rolling circle amplification | |
| TW201038942A (en) | Wireless monitoring bio-diagnosis system | |
| Catacuzzeno et al. | Energy harvesting from a bio cell | |
| CN103409427A (en) | Aptamer Ep CAM C of epithelial cell adhesion molecule, and preparation method of same | |
| CN103743802A (en) | DNA biosensor based on three-dimensional ordered gold doped nano titanium dioxide electrode as well as preparation method and application of DNA biosensor | |
| CN203662765U (en) | Passive minimally-invasive subcutaneous biochemical parameter detection chip | |
| CN104939843B (en) | Implanted cancer relapse real-time monitoring system | |
| CN101711668A (en) | Biorhythm information acquisition method | |
| Naghdi et al. | Moving toward smart biomedical sensing | |
| Sun et al. | Nanopore biphasic-pulse biosensor | |
| Cunha et al. | Machine learning for stem cell differentiation and proliferation classification on electrical impedance spectroscopy | |
| CN107714095A (en) | A kind of multi-parameter quantitatively detects the device and detection method of ovulation | |
| De Fazio et al. | An overview of technologies and devices against COVID-19 pandemic diffusion: virus detection and monitoring solutions | |
| CN204813902U (en) | Implanted cancer recurring real -time monitoring system | |
| CN110907513A (en) | Electrochemical detection method of stem cells | |
| Ghoreishizadeh et al. | An implantable bio-micro-system for drug monitoring | |
| CN105462914B (en) | The method that directional induction down producing goat hair follicle stem cells break up to hair follicle cell | |
| CN207832798U (en) | A kind of wearable biosensor | |
| CN108796037A (en) | A kind of Telomerase activity method, detection nucleic acid probe molecules and detection kit | |
| CN110487877A (en) | A method of screening anti-trioxypurine small molecule compound | |
| Olivo et al. | Electronic implants: Power delivery and management | |
| CN105087794B (en) | One kind detects kit lungy |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Implanted cancer relapse real-time monitoring system Effective date of registration: 20171212 Granted publication date: 20170517 Pledgee: Huaxia Bank Beijing branch Wanliu Limited by Share Ltd Pledgor: Beijing Yang Shen biology information technology company limited Registration number: 2017990001155 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20181206 Granted publication date: 20170517 Pledgee: Huaxia Bank Beijing branch Wanliu Limited by Share Ltd Pledgor: Beijing Yang Shen biology information technology company limited Registration number: 2017990001155 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Implanted cancer relapse real-time monitoring system Effective date of registration: 20181225 Granted publication date: 20170517 Pledgee: Huaxia Bank Beijing branch Wanliu Limited by Share Ltd Pledgor: Beijing Yang Shen biology information technology company limited Registration number: 2018990001252 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20191206 Granted publication date: 20170517 Pledgee: Huaxia Bank Beijing branch Wanliu Limited by Share Ltd Pledgor: Beijing Yang Shen biology information technology company limited Registration number: 2018990001252 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200228 Address after: Room 2503, Qianshan building, building D2, Qingdao International Innovation Park Phase II, No. 1, Keyuan Weiyi Road, Laoshan District, Qingdao, Shandong Province Patentee after: Qingdao Yangshen biomedical Co., Ltd Address before: The court building 1 cubic Beijing No. 100080 Haidian District Shanyuan street 3103 Patentee before: Beijing Yang Shen biology information technology company limited |
|
| TR01 | Transfer of patent right |