CN104904710A - Aschersonia microcapsule suspending agent and preparation method therefor - Google Patents
Aschersonia microcapsule suspending agent and preparation method therefor Download PDFInfo
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- CN104904710A CN104904710A CN201510247135.2A CN201510247135A CN104904710A CN 104904710 A CN104904710 A CN 104904710A CN 201510247135 A CN201510247135 A CN 201510247135A CN 104904710 A CN104904710 A CN 104904710A
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- suspending agent
- aschersonia
- sodium alginate
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Abstract
The invention discloses an aschersonia microcapsule suspending agent. First, conidia or hyphae of aschersonia are absorbed to form capsule cores by utilization of adsorbents, dispersion in a sodium alginate solution is carried out, plant oil and emulsifiers are added, emulsification is carried out, and therefore a W/O emulsion is formed. Then microcapsules are prepared through an interface polymerization method, sodium alginate on the emulsion interface and calcium chloride are subjected to a crosslinking polymerization reaction, sodium alginate is solidified and precipitates, finally the precipitate is dispersed in water and a suspending agent is formed. In the method, conidia or hyphae of aschersonia are in a relatively moist environment, the fact that most of aschersonia can germinate is ensured, and the invasion rate is raised. In addition, activity decline of conidia of aschersonia caused by ultraviolet ray or the like is prevented, storage time and a shelf life of aschersonia preparation can be prolonged substantially, and the aschersonia microcapsule suspending agent is a promising biocontrol agent preventing and controlling insect pests of aleyrodid and scale insects.
Description
Technical field
The present invention relates to a kind of microbial pesticide, be specifically related to a kind of seat shell spore bacteria microcapsule suspending agent and preparation method thereof.
Technical background
Seat shell spore bacterium is subordinate to Deuteromycotina (Deuteromycotina) Coelomycetes (Coelomycetes) Sphaerials (SpHareopsidales) Nectrioidaceae (Nectriadaceae), aleyrodid class, scale insect class pest important pathogen fungi, to insect, there is High pathogenicity, and to people, animal and plant nonhazardous, not killed natural enemies, free from environmental pollution, be the biological control of insect pests disease fungus that research application is both at home and abroad more at present.
In early days, carry out pest control mainly through spraying a mode for shell spore bacterium spore suspension, CN102657236A discloses a kind of seat shell spore bacterium finish, and it is made up of the pure conidial powder of seat shell spore bacterium, solvent naphtha, emulsifier, adsorbent, ultraviolet absorber and antioxidant.This finish can extend the storage period of spore, strengthens the adsorption capacity of spore.
In the process of exploitation seat shell spore bacterium biopesticide, the difficult problem faced is, conidia germination needs higher humidity, general below 80% under damp condition, conidium is difficult to sprout seat shell spore bacterium, humidity not only determines spore germination and growth, also affects a spread and epidemic for shell spore bacterium.Meanwhile, the many factors such as ultraviolet can make a shell spore bacterium conidiocidal activity decline rapidly, thus cause shelf time short, and serious meeting is inactivation in 1 to 4 month.
Summary of the invention
The object of the invention is for above-mentioned defect, provide a kind of active high, the seat shell spore bacteria microcapsule suspending agent of good stability.
A kind of seat shell spore bacteria microcapsule suspending agent, it is grouped into by the one-tenth of following percentage by weight:
Described adsorbent be selected from kaolin, diatomite, white carbon, active carbon, starch one or more;
The HLB value of described emulsifier is greater than 10.
Preferably, the percentage by weight of described composition is:
Further preferably, described suspending agent is selected from one or more in carboxymethyl cellulose, xanthans, sodium carboxymethylcellulose, gelatin, gum Arabic.
A method for described seat shell spore bacteria microcapsule suspending agent, comprises the following steps:
1) sodium alginate is soluble in water, obtain the sodium alginate aqueous solution that weight concentration is 0.5-2%, be scattered in sodium alginate aqueous solution after the conidium of seat shell spore bacterium or mycelia are mixed with adsorbent, obtain aqueous phase;
2) vegetable oil is mixed with emulsifier, obtain oil phase;
3) oil phase is joined in aqueous phase, stir at normal temperatures and form w/o type emulsion;
4) calcium chloride is soluble in water, obtain the calcium chloride solution that weight concentration is 0.1-10%, then join in emulsion, stir rapidly, make sodium alginate and calcium chloride occur to be cross-linked and be formed and precipitate;
5) by after sedimentation and filtration, washing, to be scattered in water and to add suspending agent.
Hydrophilic seat shell spore bacterium conidium or mycelia adsorbent are first formed capsule-core by the present invention, be scattered in sodium alginate soln, and add vegetable oil and emulsifier carries out emulsification, thus form w/o type emulsion, seat shell spore bacterium conidium or mycelia are wrapped in emulsion, then microcapsules are made with interfacial polymerization, sodium alginate on emulsion interface and calcium chloride generation cross-linking polymerization, sodium alginate is cured and precipitates, form microcapsules, be in relatively moistening environment from making a shell spore bacterium conidium or mycelia, ensure that seat shell spore bacterium can major part sprout, improve infection rate, also be beneficial to the seat shell spore bacterium conidiocidal activity preventing ultraviolet etc. from causing to decline simultaneously.Material used in preparation process of the present invention, can the storage time of significant prolongation seat shell spore bacteria preparation and shelf life to seat shell spore bacterium safety, nonhazardous effect.So seat shell spore bacteria microcapsule suspending agent prepared by the present invention is the biological prevention and control agent of a kind of promising control aleyrodid class, scale insect class pest.
Accompanying drawing explanation
Fig. 1 is a shell spore bacteria microcapsule suspending agent and the conidial powder germination rate change curve at lay up period.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1
A kind of seat shell spore bacteria microcapsule suspending agent, is grouped into by the one-tenth of following percentage by weight:
Described adsorbent is kaolin;
The HLB value of described emulsifier is greater than 10, is Arlacel-80;
Described suspending agent is carboxymethyl cellulose.
Preparation method is:
1) by the water-soluble solution of 0.13g sodium alginate 26g, obtain the sodium alginate aqueous solution of 0.5%, be scattered in sodium alginate aqueous solution after the conidium of 0.07g seat shell spore bacterium is mixed with 0.06g kaolin, obtain aqueous phase;
2) 21.33g soybean oil is mixed with 0.3g Arlacel-80, obtain oil phase;
3) oil phase is joined in aqueous phase, stir at normal temperatures and form w/o type emulsion;
4) 4g calcium chloride is dissolved in 100g water, obtains the calcium chloride solution of 4%, then join in emulsion, stir rapidly, make sodium alginate and calcium chloride occur to be cross-linked and be formed and precipitate;
5) by after sedimentation and filtration, washing, be scattered in water, add 0.03g carboxymethyl cellulose, and be settled to 100g with water.
Embodiment 2
A kind of seat shell spore bacteria microcapsule suspending agent, is grouped into by the one-tenth of following percentage by weight:
Described adsorbent is diatomite;
The HLB value of described emulsifier is greater than 10, is fatty alcohol-polyoxyethylene ether;
Described suspending agent is xanthans.
Preparation method is:
1) by the water-soluble solution of 0.06g sodium alginate 6g, obtain the sodium alginate aqueous solution of 1%, be scattered in sodium alginate aqueous solution after the mycelia of 0.01g seat shell spore bacterium is mixed with 0.01g diatomite, obtain aqueous phase;
2) fatty alcohol-polyoxyethylene ether that 40g olive oil and 2g HLB value are greater than 10 is mixed, obtain oil phase;
3) oil phase is joined in aqueous phase, stir at normal temperatures and form w/o type emulsion;
4) 1g calcium chloride is dissolved in 100g water, obtains the calcium chloride solution of 1%, then join in emulsion, stir rapidly, make sodium alginate and calcium chloride occur to be cross-linked and be formed and precipitate;
5) by after sedimentation and filtration, washing, be scattered in water, add 0.01g xanthans, and be settled to 100g with water.
Embodiment 3
A kind of seat shell spore bacteria microcapsule suspending agent, is grouped into by the one-tenth of following percentage by weight:
Described adsorbent is active carbon and starch, and weight ratio is 1: 1;
The HLB value of described emulsifier is greater than 10, is alkylphenol polyoxyethylene;
Described suspending agent is gelatin and gum Arabic, and weight ratio is 5: 1.
Preparation method is:
1) by the water-soluble solution of 2g sodium alginate 100g, obtain the sodium alginate aqueous solution of 2%, be scattered in sodium alginate aqueous solution after the conidium of 1g seat shell spore bacterium is mixed with 2g adsorbent, obtain aqueous phase;
2) alkylphenol polyoxyethylene that 20g corn oil and 0.1g HLB value are greater than 10 is mixed, obtain oil phase;
3) oil phase is joined in aqueous phase, stir at normal temperatures and form w/o type emulsion;
4) 8g calcium chloride is dissolved in 80g water, obtains the calcium chloride solution of 10%, then join in emulsion, stir rapidly, make sodium alginate and calcium chloride occur to be cross-linked and be formed and precipitate;
5) by after sedimentation and filtration, washing, be scattered in water, add 1g suspending agent, and be settled to 100g with water.
Test example
1. a shell spore bacteria microcapsule suspending agent is to oranges and tangerines aleyrodid pathogenicity
As shown in table 1, under 25 DEG C of conditions, during relative moisture 55%, seat shell spore bacterium spore suspension is more weak to oranges and tangerines aleyrodid infection ability, and the lethal concentration of 50 is 1.64 × 10
7individual spore/mL, and seat shell spore bacteria microcapsule suspending agent is under the same conditions, is significantly higher than spore suspension to oranges and tangerines aleyrodid infecting potential, the lethal concentration of 50 is 7.65 × 10
5individual spore/mL.After illustrating that tube base shell spore bacterium conidial powder makes micro-capsule suspension, the activity of seat shell spore bacterium spore can be improved.
A table 1 shell spore bacterium is to the lethal concentration of 50 of oranges and tangerines aleyrodid nymph
2. a shell spore bacteria microcapsule suspending agent is to ultraviolet resistance
As shown in table 2, after UV-irradiation, conidial powder survival rate reduces rapidly, when irradiation time is to 1h, and conidial powder complete deactivation, and under the same terms, micro-capsule suspension survival rate is higher, ultraviolet irradiation is after 3 hours, and survival rate still reaches 67.50%.The seat shell spore bacteria microcapsule suspending agent obtained of the present invention is under long-time ultraviolet irradiation, and survival rate is also significantly higher than conidial suspension and mycelium suspended liquid.
Survival rate assay method: preparation spore germination liquid: 2% glucose+1% tryptone, the taper bottled 9ml spore germination liquid of each 50ml, sealed membrane seals, and 122 DEG C of moist heat sterilization 30min, cool for subsequent use.In superclean bench, the conidium be emitted through by ultraviolet lighting and micro-capsule suspension are placed in germination fluid respectively.Now system concentration is 1 × 10
5individual/ml.Be placed in 100r/min, after cultivating 24h under 25 DEG C of conditions, detect germination rate, i.e. survival rate.
Table 1 ultraviolet is on the impact of conidial powder and microcapsules
3. a shell spore bacteria microcapsule bin stability
Store seat shell spore bacterium conidial powder and micro-capsule suspension at normal temperature 25 DEG C and low temperature 4 DEG C, observe germination rate.Result shows, the conidial powder germination rate of 25 DEG C of storages reduces in time gradually, after 90d, germination rate reduces 70.49%, remarkable reduction (t=8.054 compared with 0d germination rate, p<0.01), after the conidial powder 90d of 4 DEG C of storages, germination rate reduces by 9.94%, reduces not significantly (t=1.325, p>0.05).Microcapsule formulation stability is comparatively strong, and after 90d, microcapsule formulation 25 DEG C and 4 DEG C of storages reduce 4.52% and 7.90% respectively, reduces not significantly (t=1.291, p>0.05; T=1.496, p>0.05).
Claims (4)
1. a seat shell spore bacteria microcapsule suspending agent, is characterized in that: it is grouped into by the one-tenth of following percentage by weight:
Described adsorbent be selected from kaolin, diatomite, white carbon, active carbon, starch one or more;
The HLB value of described emulsifier is greater than 10.
2. as claimed in claim 1 shell spore bacteria microcapsule suspending agent, is characterized in that: the percentage by weight of described composition is:
3. as claimed in claim 1 shell spore bacteria microcapsule suspending agent, is characterized in that: described suspending agent be selected from carboxymethyl cellulose, xanthans, sodium carboxymethylcellulose, gelatin, gum Arabic one or more.
4. prepare a method for seat shell spore bacteria microcapsule suspending agent described in claim 1 or 2 or 3, it is characterized in that comprising the following steps:
1) sodium alginate is soluble in water, obtain the sodium alginate aqueous solution that weight concentration is 0.5-2%, be scattered in sodium alginate aqueous solution after the conidium of seat shell spore bacterium or mycelia are mixed with adsorbent, obtain aqueous phase;
2) vegetable oil is mixed with emulsifier, obtain oil phase;
3) oil phase is joined in aqueous phase, stir at normal temperatures and form w/o type emulsion;
4) calcium chloride is soluble in water, obtain the calcium chloride solution that weight concentration is 0.1-10%, then join in emulsion, stir rapidly, make sodium alginate and calcium chloride occur to be cross-linked and be formed and precipitate;
5) by after sedimentation and filtration, washing, to be scattered in water and to add suspending agent.
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| CN105638653A (en) * | 2015-12-29 | 2016-06-08 | 安徽美兰农业发展股份有限公司 | 45% 2-methyl-4-chloro isooctyl microcapsule suspension |
| CN105638652A (en) * | 2015-12-29 | 2016-06-08 | 安徽美兰农业发展股份有限公司 | Preparation technology of 45% 2-methyl-4-chloro isooctyl microcapsule suspension |
| CN105919864A (en) * | 2016-05-31 | 2016-09-07 | 浙江大学 | Bamboo leaf flavonoid composite nano-particles with skin whitening effect and preparation method thereof |
| CN107136123A (en) * | 2017-04-19 | 2017-09-08 | 明光市农源农作物专业合作社 | A kind of sodium alginate for preventing and treating clubroot is coated bacteria agent and preparation method thereof |
| CN107156114A (en) * | 2017-07-04 | 2017-09-15 | 广东省农业科学院植物保护研究所 | A kind of method for preparing Java Isaria micro-capsule suspension |
| CN108812706A (en) * | 2018-08-10 | 2018-11-16 | 武汉埃格瑞生物工程技术有限公司 | A kind of agricultural builder of olive oil and its preparation method and application for killing pest |
| CN111050547A (en) * | 2017-07-04 | 2020-04-21 | 盘古农用化工有限公司 | Capsule pesticide |
| CN114522670A (en) * | 2022-01-21 | 2022-05-24 | 内蒙古大学 | Microsphere and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105638653A (en) * | 2015-12-29 | 2016-06-08 | 安徽美兰农业发展股份有限公司 | 45% 2-methyl-4-chloro isooctyl microcapsule suspension |
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| CN105919864A (en) * | 2016-05-31 | 2016-09-07 | 浙江大学 | Bamboo leaf flavonoid composite nano-particles with skin whitening effect and preparation method thereof |
| CN105919864B (en) * | 2016-05-31 | 2019-01-08 | 浙江大学 | Bamboo-leaves flavones composite nanoparticle with white-skinned face function and preparation method thereof |
| CN107136123A (en) * | 2017-04-19 | 2017-09-08 | 明光市农源农作物专业合作社 | A kind of sodium alginate for preventing and treating clubroot is coated bacteria agent and preparation method thereof |
| CN107156114A (en) * | 2017-07-04 | 2017-09-15 | 广东省农业科学院植物保护研究所 | A kind of method for preparing Java Isaria micro-capsule suspension |
| CN111050547A (en) * | 2017-07-04 | 2020-04-21 | 盘古农用化工有限公司 | Capsule pesticide |
| CN107156114B (en) * | 2017-07-04 | 2021-05-04 | 广东省农业科学院植物保护研究所 | Method for preparing Isaria javanicus microcapsule suspending agent |
| CN108812706A (en) * | 2018-08-10 | 2018-11-16 | 武汉埃格瑞生物工程技术有限公司 | A kind of agricultural builder of olive oil and its preparation method and application for killing pest |
| CN114522670A (en) * | 2022-01-21 | 2022-05-24 | 内蒙古大学 | Microsphere and preparation method and application thereof |
| CN114522670B (en) * | 2022-01-21 | 2023-11-17 | 内蒙古大学 | Microsphere, preparation method and application thereof |
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