CN104897641A - Raman spectrum acquisition system with low background noise - Google Patents
Raman spectrum acquisition system with low background noise Download PDFInfo
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- CN104897641A CN104897641A CN201510248904.0A CN201510248904A CN104897641A CN 104897641 A CN104897641 A CN 104897641A CN 201510248904 A CN201510248904 A CN 201510248904A CN 104897641 A CN104897641 A CN 104897641A
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- raman spectrum
- acquisition system
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Abstract
The invention discloses a Raman spectrum acquisition system with low background noise. By collecting side Raman scattering beams, the Raman spectrum acquisition system acquires a Raman spectrum with low background noise. Through non-coaxial design of excitation light path and Raman scattering light collecting light path, the invention overcomes the problem that the traditional Raman spectrum acquisition system is likely to be interfered by excitation beam dominated strong background noise while collecting forward or backward or Raman scattering beams. At the same time, by means of vertically downward incidence of excitation beams from the position over a sample, a linear excited region can be configured, and the linear excited region matches with a slit in height. The Raman spectrum acquisition system provided by the invention makes full use of the effective range of an array detector's detection surface, increases the luminous flux of Raman scattering beams incident to the array detector, and enhances the signal to noise ratio.
Description
Technical field
The present invention relates to spectrometric instrument technical field, particularly relate to a kind of Raman spectrum acquisition system with low ground unrest.
Background technology
Raman spectrum is a kind of important molecular spectrum detection technique, there is outstanding feature, comprising: sample not to be contacted, Non-Destructive Testing, be applicable to the detection of black sample and aqueous specimen, adapt to the testing environment that high temperature, low temperature and high pressure etc. are severe, light spectrum image-forming is quick, easy, real-time.At present, Raman spectrum is widely used in medical science and the field such as pharmacy, chemistry and material science, Food Science, environmental protection, geology archaeology, criminal investigation qualification, becomes a kind of detection method reliably.
Raman spectrum is the spectrum detection technique based on Raman scattering effect, is irradiated to and material occurs for photon and intermolecular energy exchange and the inelastic scattering produced, identify different material molecular structures, distinguish different material molecules according to exciting light.Meanwhile, in conjunction with the chemometrics method of development, realize the Accurate Determining of material component content information, the target reaching qualitative analysis and quantitatively detect.
Raman scattering comprises the stokes scattering of corresponding scattered photon energy reduction and the anti Stokes scattering of energy increase, according to Boltzmann distribution law, the intensity of stokes scattering is much larger than the intensity of anti Stokes scattering, therefore, Raman spectrum acquisition system mainly collects Stokes ratio bundle.But the intensity of Raman diffused light is only 10 of excitating light strength
-6doubly, thus, be subject to the strong background noise interference based on excitation beam when traditional Raman spectrum acquisition system collects forward direction or backward Raman scattering light beam, cause Raman spectral information to be submerged in strong background noise.
Summary of the invention
The invention provides a kind of Raman spectrum acquisition system with low ground unrest, the present invention reduces the interference that excitation beam gathers Raman spectrum, improves signal to noise ratio (S/N ratio), described below:
A kind of Raman spectrum acquisition system with low ground unrest, comprise: continuous wave laser, the first optical filter, the first convergent lens, plane mirror, sample cell, to it is characterized in that, described Raman spectrum acquisition system also comprises: the second convergent lens, the 3rd convergent lens, the second optical filter, slit, light-splitting device and array detecting device
Described continuous wave laser produces excitation beam, and described first optical filter is bandpass filter, for the parasitic light of continuous wave laser described in filtering itself;
Described first convergent lens is assembled excitation beam, and by described plane mirror turnover excitation beam, excitation beam incides downwards sample from the upper vertical of described sample cell, inspires Raman scattering light beam at described sample cell place;
The wire at described sample cell place is stimulated region by described second convergent lens, described 3rd single-convergent lens imaging at described slit place;
Described second optical filter filtering is mixed in the intrafascicular excitation beam of side direction Raman diffused light and Rayleigh scattering light beam;
Described slit is for limiting the width of the side direction Raman scattering light beam that can pass through; Described light-splitting device is used for side direction Raman diffused light Shu Jinhang light splitting, and the side direction Raman scattering light beam of different frequency incides the diverse location of described array detecting device;
Described array detecting device converts described side direction Raman scattering light beam to electric signal, collects Raman spectrum.
Preferably, described excitation beam incides vertically downward sample directly over described sample cell.
Preferably, described sample cell and described slit are Nonimage Conjugate Relations.
Wherein, the focal length of described 3rd convergent lens is not more than the focal length of described second convergent lens.
Preferably, the be stimulated height in region and the height of described slit of described wire matches.Described sample cell is positioned at the object space focal plane place of described second convergent lens, and described slit is positioned at the image space focal plane place of described 3rd convergent lens.
The beneficial effect of technical scheme provided by the invention is: relative to traditional Raman spectrum acquisition system of collecting forward direction or backward Raman scattering light beam, collection side direction Raman scattering light beam can reduce the interference that excitation beam gathers Raman spectrum effectively, obtains the Raman spectrum with low ground unrest; Simultaneously, expand the region that is stimulated producing Raman scattering light beam, the be stimulated height in region and slit height of wire matches, and can make full use of the effective range of array detecting device detection faces, increase the luminous flux inciding the Raman scattering light beam of detecting device, improve signal to noise ratio (S/N ratio).
Accompanying drawing explanation
Fig. 1 is a kind of structural representation with the Raman spectrum acquisition system of low ground unrest;
Fig. 2 is that sample cell place is stimulated the schematic diagram that region and slit match.
In accompanying drawing, the list of parts representated by each label is as follows:
1: continuous wave laser; 2: the first optical filters;
3: the first convergent lenses; 4: plane mirror;
5: sample cell; 6: the second convergent lenses;
7: the three convergent lenses; 8: the second optical filters;
9: slit; 10: light-splitting device;
11: array detecting device.
Embodiment
For making the object, technical solutions and advantages of the present invention clearly, below embodiment of the present invention is described further in detail.
In order to solve the deficiency existed in background technology, therefore, need the new approaches proposing the Raman spectrum acquisition system of collecting side direction Raman scattering light beam, the non co axial being collected light path by exciting light light path and Raman diffused light is designed, effectively reduce the interference that excitation beam gathers Raman spectrum, obtain the Raman spectrum with low ground unrest.On this basis, structure wire is stimulated region, matches, make full use of the effective range of array detecting device detection faces with slit height, increases the luminous flux inciding the Raman scattering light beam of detecting device, improves signal to noise ratio (S/N ratio).
Embodiment 1
A kind of Raman spectrum acquisition system with low ground unrest, see Fig. 1, comprising: continuous wave laser 1, first optical filter 2, first convergent lens 3, plane mirror 4, sample cell 5, second convergent lens 6, the 3rd convergent lens 7, second optical filter 8, slit 9, light-splitting device 10 and array detecting device 11.
Continuous wave laser 1 produces the excitation beam of high power, narrow linewidth, and the first optical filter 2 is bandpass filter, for the parasitic light of filtering continuous wave laser 1 itself.First convergent lens 3 pairs excitation beam is assembled, to be transferred excitation beam by plane mirror 4, excitation beam is incided sample vertically downward from the top (preferably) of sample cell 5, and at sample cell 5, place inspires Raman scattering light beam.
The wire at the sample cell 5 place region that is stimulated is imaged on slit 9 place by the second convergent lens 6, the 3rd convergent lens 7, the be stimulated height in region and the height of slit 9 of wire matches, and sample cell 5 and slit 9 are in Nonimage Conjugate Relations (adopting * to represent in Fig. 1).Sample cell 5 is positioned at the object space focal plane place of the second convergent lens 6, and slit 9 is positioned at the image space focal plane place of the 3rd convergent lens 7.Second optical filter 8 filtering is mixed in the intrafascicular excitation beam of side direction Raman diffused light and Rayleigh scattering light beam.
Slit 9 limits the width of the side direction Raman scattering light beam that can pass through, and affects spectral resolution, narrower width, corresponding higher resolution.Light-splitting device 10 couples of side direction Raman diffused light Shu Jinhang light splitting, makes the light beam of different frequency incide the diverse location of array detecting device 11.Array detecting device 11 can use charge coupled cell (CCD detecting device), converts side direction Raman scattering light beam to electric signal, collects Raman spectrum.
Embodiment 2
A kind of Raman spectrum acquisition system with low ground unrest, see Fig. 1 and Fig. 2, comprising: continuous wave laser 1, first optical filter 2, first convergent lens 3, plane mirror 4, sample cell 5, second convergent lens 6, the 3rd convergent lens 7, second optical filter 8, slit 9, light-splitting device 10 and array detecting device 11.
Continuous wave laser 1 produces that centre wavelength is 532nm, power is not less than 50mW and live width is not more than the excitation beam of 0.6nm.First optical filter 2 is bandpass filter, for the parasitic light of filtering continuous wave laser 1 itself.First convergent lens 3 pairs excitation beam is assembled, to be transferred excitation beam by plane mirror 4, excitation beam is incided sample vertically downward from the top (preferably) of sample cell 5, and at sample cell 5, place inspires Raman scattering light beam.Subsequent optical path mainly collects side direction Raman scattering light beam, and the non co axial that formation exciting light light path and Raman diffused light collect light path designs.Optical axis L and optical axis H, not in same level, have different vertical heights, and optical axis H is higher than optical axis L.
Wherein, the focus of the first convergent lens 3 is positioned at the middle part of sample cell 5, and, first convergent lens 3 has longer focal length, at focus place, there is less convergent angle, (concrete length sets according to the needs in practical application, and the embodiment of the present invention does not limit this) can realize excitation beam in the certain limit of focus both sides to be had enough energy excitations and goes out Raman scattering light beam.Now, the side direction Raman scattering light beam of sample is not only excited by the excitation beam of the first convergent lens 3 focus, but by focus and both sides thereof among a small circle in excitation beam excite, can think that side direction Raman scattering light beam to be collected derives from vertically, the region that is stimulated of linearity.
The wire at the sample cell 5 place region that is stimulated is imaged on slit 9 place by the second convergent lens 6, the 3rd convergent lens 7, the be stimulated height in region and the height of slit 9 of wire matches, (when matched, the light beam collected is maximum, and signal to noise ratio (S/N ratio) is the highest) sample cell 5 and slit 9 be in Nonimage Conjugate Relations (adopting * to represent in Fig. 1).The plane A at sample cell 5 place and the plane B at slit 9 place is all vertical planes, cuts and is parallel to each other, and perpendicular to optical axis L; Sample cell 5 is positioned at the object space focal plane place of the second convergent lens 6, and slit 9 is positioned at the image space focal plane place of the 3rd convergent lens 7.Second optical filter 8 filtering is mixed in the intrafascicular excitation beam of side direction Raman diffused light and Rayleigh scattering light beam.
Wherein, in order to collect side direction Raman scattering light beam as much as possible, improve signal to noise ratio (S/N ratio), the focal length of the second convergent lens 6 is less, spacing between second convergent lens 6, the 3rd convergent lens 7 is as far as possible little, and the focal length of the 3rd convergent lens 7 is not more than the focal length of the second convergent lens 6.
Slit 9 limits the width of the side direction Raman scattering light beam that can pass through, and affects spectral resolution, narrower width, corresponding higher resolution.Light-splitting device 10 couples of side direction Raman diffused light Shu Jinhang light splitting, makes the light beam of different frequency incide the diverse location of array detecting device 11.Array detecting device 11 can use charge coupled cell (CCD detecting device), converts side direction Raman scattering light beam to electric signal, collects Raman spectrum.
The embodiment of the present invention is to the model of each device except doing specified otherwise, and the model of other devices does not limit, as long as can complete the device of above-mentioned functions.
It will be appreciated by those skilled in the art that accompanying drawing is the schematic diagram of a preferred embodiment, the invention described above embodiment sequence number, just to describing, does not represent the quality of embodiment.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. one kind has the Raman spectrum acquisition system of low ground unrest, comprise: continuous wave laser, the first optical filter, the first convergent lens, plane mirror, sample cell, to it is characterized in that, described Raman spectrum acquisition system also comprises: the second convergent lens, the 3rd convergent lens, the second optical filter, slit, light-splitting device and array detecting device
Described continuous wave laser produces excitation beam, and described first optical filter is bandpass filter, for the parasitic light of continuous wave laser described in filtering itself;
Described first convergent lens is assembled excitation beam, and by described plane mirror turnover excitation beam, excitation beam incides downwards sample from the upper vertical of described sample cell, inspires Raman scattering light beam at described sample cell place;
The wire at described sample cell place is stimulated region by described second convergent lens, described 3rd single-convergent lens imaging at described slit place;
Described second optical filter filtering is mixed in the intrafascicular excitation beam of side direction Raman diffused light and Rayleigh scattering light beam;
Described slit is for limiting the width of the side direction Raman scattering light beam that can pass through; Described light-splitting device is used for side direction Raman diffused light Shu Jinhang light splitting, and the side direction Raman scattering light beam of different frequency incides the diverse location of described array detecting device;
Described array detecting device converts described side direction Raman scattering light beam to electric signal, collects Raman spectrum.
2. a kind of Raman spectrum acquisition system with low ground unrest according to claim 1, it is characterized in that, described excitation beam incides vertically downward sample directly over described sample cell.
3. a kind of Raman spectrum acquisition system with low ground unrest according to claim 1, it is characterized in that, described sample cell and described slit are Nonimage Conjugate Relations.
4. a kind of Raman spectrum acquisition system with low ground unrest according to claim 1, is characterized in that, the focal length of described 3rd convergent lens is not more than the focal length of described second convergent lens.
5. a kind of Raman spectrum acquisition system with low ground unrest according to claim 1, is characterized in that, the be stimulated height in region and the height of described slit of described wire matches.
6. a kind of Raman spectrum acquisition system with low ground unrest according to claim 1, is characterized in that, described sample cell is positioned at the object space focal plane place of described second convergent lens, and described slit is positioned at the image space focal plane place of described 3rd convergent lens.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106706597A (en) * | 2017-01-10 | 2017-05-24 | 上海理工大学 | Device and method for detecting platelet-derived growth factor based on raman spectrum |
| CN109374528A (en) * | 2018-11-25 | 2019-02-22 | 金华职业技术学院 | A kind of spectral measurement method under condition of high voltage |
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| CN1159578A (en) * | 1995-09-20 | 1997-09-17 | 株式会社京都第一科学 | Testing method utilizing scattered light and testing device thereof |
| CN102410998A (en) * | 2011-12-16 | 2012-04-11 | 湖南科技大学 | Laser light scattering (LLS)-Raman spectrum joint device |
| CN102495041A (en) * | 2011-12-08 | 2012-06-13 | 吉林大学 | Optical diagnostic system on basis of laser spontaneous Raman scattered ray imaging |
| CN103822910A (en) * | 2012-11-16 | 2014-05-28 | 福州高意通讯有限公司 | Raman probes for miniature Raman spectrometer |
| US20140252234A1 (en) * | 2013-03-05 | 2014-09-11 | Smiths Detection Inc. | Transmission raman sample analysis |
| CN104458696A (en) * | 2014-12-02 | 2015-03-25 | 天津大学 | Digital micro-mirror element based micro curing raman spectrometer |
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2015
- 2015-05-15 CN CN201510248904.0A patent/CN104897641A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1159578A (en) * | 1995-09-20 | 1997-09-17 | 株式会社京都第一科学 | Testing method utilizing scattered light and testing device thereof |
| CN102495041A (en) * | 2011-12-08 | 2012-06-13 | 吉林大学 | Optical diagnostic system on basis of laser spontaneous Raman scattered ray imaging |
| CN102410998A (en) * | 2011-12-16 | 2012-04-11 | 湖南科技大学 | Laser light scattering (LLS)-Raman spectrum joint device |
| CN103822910A (en) * | 2012-11-16 | 2014-05-28 | 福州高意通讯有限公司 | Raman probes for miniature Raman spectrometer |
| US20140252234A1 (en) * | 2013-03-05 | 2014-09-11 | Smiths Detection Inc. | Transmission raman sample analysis |
| CN104458696A (en) * | 2014-12-02 | 2015-03-25 | 天津大学 | Digital micro-mirror element based micro curing raman spectrometer |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106706597A (en) * | 2017-01-10 | 2017-05-24 | 上海理工大学 | Device and method for detecting platelet-derived growth factor based on raman spectrum |
| CN109374528A (en) * | 2018-11-25 | 2019-02-22 | 金华职业技术学院 | A kind of spectral measurement method under condition of high voltage |
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