CN104845879A - Automatic sampling system and control method of fermentation tank - Google Patents
Automatic sampling system and control method of fermentation tank Download PDFInfo
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- CN104845879A CN104845879A CN201510290912.1A CN201510290912A CN104845879A CN 104845879 A CN104845879 A CN 104845879A CN 201510290912 A CN201510290912 A CN 201510290912A CN 104845879 A CN104845879 A CN 104845879A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
Abstract
The invention discloses an automatic sampling system and a control method of a fermentation tank. In a sampling process, a sampling valve is controlled to be opened, a pipeline valve is controlled to be opened and a bidirectional sampling pump is controlled to be opened by a controller. When a fermentation liquor sample is detected by a liquid sensor, the controller starts timing and delays a certain time to control the sampling valve to be closed, and simultaneously controls a blowing pump and a blowing valve to be opened, and the sample in a delivery pipe is sent to a sampling pool by the sterile air through a sterile air pipe. When the fermentation liquor sample is detected by an electro-conductive liquid sensor in the sampling pool, the controller controls the bidirectional sampling pump to be closed and controls a waste solution valve to be opened, and the redundant fermentation liquor sample in the delivery pipe is discharged to a waste solution bottle through a waste solution pipeline. After the fermentation liquor sample is processed, the controller controls the waste solution valve to be opened and controls the bidirectional sampling pump to reversely rotate, and the fermentation liquor sample in the sampling pool is discharged to the waste solution bottle through the waste solution pipeline. The automatic sampling system is suitable for various fermentation sampling devices, various fermentation tanks can be automatically sampled, and thus an on-line analyzing system can be applied to various fermentation types.
Description
Technical field
The present invention relates to a kind of automatic sampling system and control method of fermentor tank.
Background technology
Want timing from fermentor tank sampling analysis in fermenting process, obtain the various critical datas of fermenting process.In fermentor tank, gather the fermented sample most important point is to ensure safety of fermenting, and prevents the living contaminants fermented liquid in environment.Different according to the target of fermentation, tunning state difference is very big, and some final products are very rare, and what have is then very thick, the even agglomerating one-tenth block had.Some fermenting processs were less than one day, and some needs tens of sky.Fermentor tank is also of a great variety, different sizes.Large tankage can reach hundreds of cubic meter, and little only has several liter.For different fermented types and fermentor tank, different analyses measures requirement, just has different requirements to the automatic sampling of fermenting process.Current safest sampling side is micropore ceramic tube sterile sampling a bit, because thalline is not by micropore, so can not fermented liquid be polluted, but its shortcoming is also obvious, not only sample rate is slow, and easily blocks, and is secondly the indirect sampling system that simulation manually samples, because all use steam sterilizing thief hole after every sub-sampling, security is also very high.But this method sampling amount is more, each sampling amount at least wants tens of milliliter, is not suitable for the small fermentor only having tens of liter.The method of direct sampling in tank, sample rate is fast, and sampling amount is few, concerning fermentation period reaches the fermentation of tens of days, although there is uncontaminated air in stopple coupon to do to isolate, still has certain Pollution risk, needs to carry out sampling line sterilising treatment in the middle of fermentation.
Summary of the invention
The present invention, in order to overcome the deficiency of above technology, provides and a kind ofly guarantees to sample safely, prevents from sampling automatic sampling system and the control method polluting and be applicable to the fermentor tank of various fermented type.
The present invention overcomes the technical scheme that its technical problem adopts:
An automatic sampling system for fermentor tank, comprises
Sampling unit, is connected to fermentor tank through sampling valve, for being taken out from fermentor tank by fermented liquid;
Sampling pond, its inside is provided with the conductance type liquid sensor for tracer liquid;
Transfer lime, its one end by the road valve is connected to sampling pond by two-way sampling pump, and the other end is connected to sampling unit through liquid sensor;
Waste-solution line, its one end is connected to sampling pond through waist valve by two-way sampling pump, and the other end is connected to waste liquid bottle;
And sterile air pipeline, it is connected on the transfer lime between sampling unit and two-way sampling pump, and described sterile air pipeline is provided with purge valve and blower pump;
Described sampling valve, blower pump, purge valve, liquid sensor, conductance type liquid sensor, two-way sampling pump and pipeline valve are all connected to controlling machine.
Above-mentioned sampling unit is U-tube, and described transfer lime is connected bottom with u bent tube position.
Above-mentioned sampling unit is insert vitrified pipe sampler in fermentor tank or straight tube sampler.
Conveniently connect, above-mentioned transfer lime is sebific duct.
In order to the pipeline other to analyser carries out disinfection, also comprise disinfection cleaning solution pipeline II 19, described disinfection cleaning solution pipeline II 19 is connected to two-way sampling pump 16 through sterilization cleaning valve II 20, and described sterilization cleaning valve II 20 is connected to controlling machine.
In order to prevent in U-tube, fermentation broth sample is by the fermented liquid in living contaminants and then pollution fermentor tank, and also comprise the vapour pipe 4 be connected in U-tube 3, described vapour pipe 4 is provided with steam valve 5, described steam valve 5 is connected to controlling machine.
In order to guarantee to sample safety further, also comprise disinfection cleaning solution pipeline I 12, described disinfection cleaning solution pipeline I 12 is connected to transfer lime 9 through sterilization cleaning valve I 13.
A control method for the automatic sampling system of fermentor tank, comprises the steps:
A) controlling machine controls opening with the tank connected sampling valve of fermentation of required sampling, and the pipeline valve controlling this sampling line is opened, and controls two-way sampling pump and opens, and the fermentation broth sample in fermentor tank flows to sampling pond by sampling unit through transfer lime;
B) when the liquid sensor in transfer lime detects fermentation broth sample, controlling machine starts timing, after controlling machine time delay T1 second, controls sampling valve and closes, control blower pump and purge valve unlatching, the sample in transfer lime is sent into sampling pond by sterile air pipeline by sterile air simultaneously;
C) when sampling the conductance type liquid sensor in pond and fermentation broth sample being detected, controlling machine controls two-way sampling pump and closes and control waist valve unlatching, the unnecessary fermentation broth sample of transfer lime enters in waste liquid bottle through waste-solution line under the pressure of sterile air, after controlling machine time delay T2 second, control blower pump, purge valve, waist valve and pipeline valve to close, complete primary sample;
D) after needing repeated sampling or fermentation broth sample process to complete, controlling machine controls waist valve and opens, and controls two-way sampling pump and rotates backward, and is entered in waste liquid bottle by the fermentation broth sample in sampling pond by waste-solution line.
In order to prevent the remaining influence of previous sample fermented liquid in pipeline: every sub-sampling can repeating step a) to steps d), 2-3 time, to get rid of the impact of debris of sampling last time.
In order to control the sampling amount of fermented liquid, when fermentor tank be miniature fermenter or small-sized fermentation tank time, execution step a) before and sampling terminate rear controlling machine control sampling valve, purge valve and blower pump open, by fermentor tank side stopple coupon in fermentation broth sample be oppositely recycled in fermentor tank.
When sampling unit is U-tube, before execution step a), controlling machine controls sampling valve, blower pump and purge valve and opens, and time delay T3 performs step a) after second again, and step a) middle sampling valve is no longer opened; When having repeated steps d) time, in order to preventing pollution, the steam valve that controlling machine controls vapour pipe is opened, and controls blower pump and purge valve simultaneously and open, and after time delay T4 second, controlling machine controls steam valve, blower pump and purge valve and closes.
Influencing each other of Shi Ge road sample is sampled in order to prevent multichannel, at execution of step d) after, controlling machine controls sterilization cleaning valve II and opens and the startup of two-way sampling pump, and thimerosal is entered by disinfection cleaning solution pipeline II and is transported in sampling pond, for cleaning sampling pond.
When sampling the gap detected, controlling machine allows to manually boot the clear pond to sampling line and sterilization, controlling machine pilot piping valve is opened and two-way sampling pump starts sampling, disinfection cleaning solution is by disinfection cleaning solution pipeline I, the Chang Kaiguan road of sampling valve and purge valve, transfer lime arrives in sampling pond, after sampling pond conductance type liquid sensor detects cleaning disinfection liquid, controller is blinded off a line valve, close two-way sampling pump, after time delay T4 second, waist valve opened by controller, control the reversion of two-way sampling pump, open pipeline valve, open blower pump and purge valve, during to setting-up time, cleaning disinfection liquid in sampling pond and sampling line is entered waste liquid bottle through sewer pipe
The invention has the beneficial effects as follows: when needs are sampled, controlling machine controls opening with the tank connected sampling valve of fermentation of required sampling, the pipeline valve controlling this sampling line is opened, and controls two-way sampling pump and opens, and the fermentation broth sample in fermentor tank is flowed in sampling pond through transfer lime by sampling unit.When the liquid sensor in transfer lime detects fermentation broth sample, controlling machine starts timing and controls sampling valve after time delay certain hour to close, control blower pump and purge valve unlatching, the sample in transfer lime is sent into sampling pond by sterile air pipeline by sterile air simultaneously.Therefore achieve and control sampling quantity by timing method.When sampling the conductance type liquid sensor in pond and fermentation broth sample being detected, controlling machine controls two-way sampling pump and closes and control waist valve unlatching, the unnecessary fermentation broth sample of transfer lime enters in waste liquid bottle through waste-solution line under the pressure of sterile air, after controlling machine time delay certain hour, control blower pump, purge valve, waist valve and pipeline valve to close, complete primary sample.After fermentation broth sample process completes, controlling machine controls waist valve and opens, and controls two-way sampling pump and rotates backward, and is entered in waste liquid bottle by the fermentation broth sample in sampling pond by waste-solution line.Achieve and automatically control to sample the fermentation broth sample in multiple fermentor tank, and after sampling terminates automatic drain, realize the emptying rate of liquid in transfer lime, sampling can repeat 2-3 time simultaneously, ensure that the fermentation broth sample gathered in sampling pond is exemplary fermentation sample.The fermentor tank that the present invention is applicable to multiple sampling unit carries out automatic sampling, and makes on-line analysis system can be applied to various fermented type.
Accompanying drawing explanation
Fig. 1 is U-tube sampling structure schematic diagram of the present invention;
Fig. 2 is vitrified pipe of the present invention or straight tube sampling structure schematic diagram;
Fig. 3 is analyser lateral line connection diagram of the present invention;
In figure, 1. fermentor tank 2. sampling valve 3.U type pipe 4. vapour pipe 5. steam valve 6. sterile air pipeline 7. blower pump 8. purge valve 9. transfer lime 10. liquid sensor 11. vitrified pipe sampler 12. disinfection cleaning solution pipeline I 13. sterilization cleaning valve I 14. samples pond 15. conductance type liquid sensor 16. two-way sampling pump 17. waste-solution line 18. waist valve 19. disinfection cleaning solution pipeline II 20. sterilization cleaning valve II 21. pipeline valve.
Embodiment
Below in conjunction with accompanying drawing 1, accompanying drawing 2, the present invention will be further described for accompanying drawing 3.
An automatic sampling system for fermentor tank, comprising: sampling unit, is connected to fermentor tank 1 through sampling valve 2, for being taken out from fermentor tank 1 by fermented liquid; Sampling pond 14, its inside is provided with the conductance type liquid sensor 15 for tracer liquid; Transfer lime 9, its one end by the road valve 21 is connected to sampling pond 14 by two-way sampling pump 16, and the other end is connected to sampling unit through liquid sensor 10; Waste-solution line 17, its one end is connected to sampling pond 14 through waist valve 18 by two-way sampling pump 16, and the other end is connected to waste liquid bottle; And sterile air pipeline 6, it is connected on the transfer lime 9 between sampling unit and two-way sampling pump 16, sterile air pipeline 6 is provided with purge valve 8 and blower pump 7; Sampling valve 2, blower pump 7, purge valve 8, liquid sensor 10, conductance type liquid sensor 15, two-way sampling pump 16 and pipeline valve 21 are all connected to controlling machine.When needs are sampled, the sampling valve 2 be connected with fermentor tank 1 that controlling machine controls required sampling is opened, the pipeline valve 21 controlling this sampling line is opened, and controls two-way sampling pump 16 and opens, and the fermentation broth sample in fermentor tank 1 flows to sampling pond 14 by sampling unit through transfer lime 9.When the liquid sensor 10 in transfer lime 9 detects fermentation broth sample, controlling machine starts timing and controls sampling valve 2 after time delay certain hour to close, control blower pump 7 and purge valve 8 are opened simultaneously, and the sample in transfer lime 9 is sent into sampling pond 14 by sterile air pipeline 6 by sterile air.Therefore achieve and control sampling quantity by timing method.When sampling the conductance type liquid sensor 15 in pond 14 and fermentation broth sample being detected, controlling machine controls two-way sampling pump 16 and closes and control waist valve 18 and open, the unnecessary fermentation broth sample of transfer lime 9 enters in waste liquid bottle through waste-solution line 17 under the pressure of sterile air, after controlling machine time delay certain hour, control blower pump 7, purge valve 8, waist valve 18 and pipeline valve 21 to close, complete primary sample.Above-mentioned sampling process can repeat 2-3 time, also can not repeat.After fermentation broth sample process completes or when needing to repeat employing, controlling machine controls waist valve 18 and opens, and controls two-way sampling pump 16 and rotates backward, and is entered in waste liquid bottle by the fermentation broth sample in sampling pond 14 by waste-solution line.Achieve and automatically control to sample the fermentation broth sample in multiple fermentor tank 1, and after sampling terminates automatic drain, realize the emptying rate of liquid in transfer lime 9, sampling the fermentation broth sample gathered in pond 14 is exemplary fermentation sample to allow 2-3 repeated sampling to ensure simultaneously.The fermentor tank that the present invention is applicable to multiple sampling unit carries out automatic sampling, and makes on-line analysis system can be applied to various fermented type.
Sampling unit can be U-tube 3, and transfer lime 9 is connected bottom with U-tube 3 crooked position.Transfer lime 9 extracts fermentation broth sample bottom U-tube 3, indirect sampling is sampled as in U-tube 3, sampling valve 2 certain hour is first opened before sampling, ensure to retain exemplary fermentation liquid sample in U-tube 3, sampling valve 2 is no longer opened during subsequent samples, directly sample bottom U-tube 3, after sampling process completes, open steam valve 5 certain hour, raffinate in U-tube 3 is thoroughly got rid of, and sterilising treatment is carried out to U-tube 3, bottom U-tube 3, sampling can also get rid of the impact of the bubble in fermented liquid on sampling amount, and the sampling amount of every sub-sampling is more accurate.Sampling unit can be also the vitrified pipe sampler 11 in insertion fermentor tank 1 or straight tube sampler, and to be applicable to small-sized fermentation tank, vitrified pipe sampler can also filter, and realizes acellular sampling.Further, transfer lime 9 is sebific duct.Because sebific duct has good flexural deformation performance, therefore convenient by sampling unit with sample pond and carry out system and be connected and use pressure-pipe type control valve switch pipeline.
Can also comprise disinfection cleaning solution pipeline II 19, disinfection cleaning solution pipeline II 19 is connected to two-way sampling pump 16 through sterilization cleaning valve II 20, and sterilization cleaning valve II 20 is connected to controlling machine.When analysis mensuration completes, after sampling pond is emptying, controlling machine can control sterilization cleaning valve II 20 and open, under the effect of two-way sampling pump 16, thimerosal enters disinfection in sampling pond 14 by the transfer lime 9 on disinfection cleaning solution pipeline II 19 and analyser side, the impact of sample carryover when preventing multiple tank from sampling, clean rear controlling machine to control waist valve 18 and to open and sampling pump 16 reverses certain hour, cleaning disinfection liquid is entered waste liquid bottle, cleaning disinfection liquid remaining in a bit of sampling line is thoroughly isolated the sterile air of sampling line and outside, further ensure that sampling security.Can also comprise the vapour pipe 4 be connected in U-tube 3, vapour pipe 4 be provided with steam valve 5, steam valve 5 is connected to controlling machine.After sampling terminates, the steam valve 5 that controlling machine controls on vapour pipe 4 is opened, and control blower pump 7 and purge valve 8 are opened simultaneously, and after time delay certain hour, controlling machine controls steam valve 5, blower pump 7 and purge valve 8 and closes.Play the object utilizing high-temperature steam to wash away U-tube 3, for U-tube 3 carries out sterilizing, the fermented liquid in prevention U-tube 3 is by living contaminants thus the fermented liquid polluted in fermentor tank 1.Further, can also comprise disinfection cleaning solution pipeline I 12, disinfection cleaning solution pipeline I 12 is connected to transfer lime 9 through sterilization cleaning valve I 13.When fermentation period is very long, in the gap phase of sampling analysis, allow manually to carry out sampling line cleaning and sterilizing.When performing Manual-cleaning sterilization, controlling machine pilot piping valve 21 is opened and two-way sampling pump 16 starts sampling, and disinfection cleaning solution is arrived in sampling pond 14 by the Chang Kaiguan road of disinfection cleaning solution pipeline I 12, sampling valve 2 and purge valve 8, transfer lime 9.After sampling pond 14 conductance type liquid sensor 15 detects cleaning disinfection liquid, controller is blinded off a line valve 21 and two-way sampling pump 16, time delay certain hour kills the thalline in pipeline by cleaning disinfection liquid, after time delay, waist valve 18 opened by controller, two-way sampling pump 16 is reversed, open pipeline valve 21, blower pump 7 and purge valve 8, during to setting-up time, discharge sampling pond 14 and sampling line in cleaning disinfection liquid to waste liquid bottle.During manual pipeline sterilizing, sampling operation forbidden by controller.
The invention still further relates to a kind of control method of automatic sampling system of fermentor tank, comprise the steps:
A) sampling valve 2 be connected with fermentor tank 1 of the required sampling of controlling machine control is opened, and the pipeline valve 21 controlling this sampling line is opened, and controls two-way sampling pump 16 and opens, and the fermentation broth sample in fermentor tank 1 flows to sampling pond 14 by sampling unit through transfer lime 9.B) when the liquid sensor 10 in transfer lime 9 detects fermentation broth sample, controlling machine starts timing, after controlling machine time delay T1 second, control sampling valve 2 to close, control blower pump 7 and purge valve 8 are opened simultaneously, and the sample in transfer lime 9 is sent into sampling pond 14 by sterile air pipeline 6 by sterile air.Therefore achieve and control sampling quantity by timing method.C) when sampling the conductance type liquid sensor 15 in pond 14 and fermentation broth sample being detected, controlling machine controls two-way sampling pump 16 and closes and control waist valve 18 and open, the unnecessary fermentation broth sample of transfer lime 9 enters in waste liquid bottle through waste-solution line 17 under the pressure of sterile air, after controlling machine time delay T2 second, control blower pump 7, purge valve 8, waist valve 18 and pipeline valve 21 to close, complete primary sample.For ensureing to get exemplary fermentation sample, sampling process allows to repeat 2-3 time, also can not repeat.D) after needing repeated sampling or fermentation broth sample process to complete, controlling machine controls waist valve 18 and opens, and controls two-way sampling pump 16 and rotates backward, and is entered in waste liquid bottle by the fermentation broth sample in sampling pond 14 by waste-solution line.Achieve and automatically control to sample the fermentation broth sample in a fermentor tank 1, and after sampling terminates automatic drain, realize the emptying rate of liquid in transfer lime 9 simultaneously, allow repeated sampling 2-3 time, can ensure that sampling the fermentation broth sample gathered in pond 14 is exemplary fermentation sample.Sampling process, by opening different pipeline valves 21, and switch sampling valve 2, blower pump 7, purge valve 8, steam valve 5, liquid sensor 10 control output to different fermentor tanks, point time-sampling to different fermentations tank can be realized.
For fermentor tank 11 be miniature fermenter or small-sized fermentation tank time, because its tankage is little, need to control fermented liquid sampling amount, therefore before execution step a) and sampling terminate rear controlling machine and control sampling valve 2, purge valve 8 and blower pump 7 and open, by the reverse blowback of fermentation broth sample in stopple coupon by fermentor tank 1 in fermentor tank 1.Controlling by increasing blowback before sampling and after sampling, effectively can reduce the sampling amount of fermented liquid.
Further, when sampling unit is U-tube, before execution step a), controlling machine controls sampling valve 2, blower pump 7 and purge valve 8 and opens, time delay T3 second, perform step a) again after making to be full of exemplary fermentation sample in U-tube 3, the effect of opening purge valve 8 and blower pump 7 is that the raffinate in order to retain in U-tube 3 pipeline when preventing from starting to sample enters and is transported to 9, ensure gather fermentation broth sample be exemplary fermentation sample, step a) in no longer open sampling valve 2.When having repeated steps d) time (sampling complete after), the steam valve 5 that controlling machine controls on vapour pipe 4 is opened, and controls blower pump 7 and purge valve 8 is opened simultaneously, and after time delay T4 second, controlling machine controls steam valve 5, blower pump 7 and purge valve 8 and closes.Utilize high-temperature steam to wash away U-tube 3, for U-tube 3 carries out sterilizing, the fermented liquid in prevention U-tube 3 is by living contaminants thus the fermented liquid polluted in fermentor tank 1.
For the direct sampling of straight tube sampler, it is the key successfully sampled that anti-sampling is polluted, because stopple coupon is directly communicated with fermented liquid, sampling line needs together with fermentation raw material sterilising treatment completely before fermentation starts, for disinfection fermentation tank of offing normal, pipeline before many connectors after tubing clamp pipe valve can be unloaded from pinch off valve, together with fermentor tank 1 sterilizing.For disinfection fermentation tank in place, need pipeline to take off from pinch valve and two-way sampling pump 16, pass into steam when sterile air pipeline from uncontaminated air ingress, be insurance, first pipeline can be unloaded before this and carry out a sterilising treatment.Be full of sterile air in sampling line after final sampling completes, not with the fermentation broth sample of nutrition, can prevent sampling from polluting.Simultaneously when sampling unit is straight tube sampler, at execution of step d) after, controlling machine can control the unlatching of sterilization cleaning valve II 20 and two-way sampling pump 16 opens the several seconds, and thimerosal is entered by disinfection cleaning solution pipeline II 19 and is transported to 9 near two-way sampling pump 16 position.Like this in the gap phase of sampling, seal sampling line by thimerosal, guarantee to sample safety.Can sample pond with thimerosal cleaning after mensuration terminates, when preventing multiple tank from sampling, each tank sample influences each other.During cleaning, cleaning disinfection liquid enters in sampling pond 14 by disinfection cleaning solution pipeline II 19, after sampling pond conductance type liquid sensor 15 detects cleaning disinfection liquid, controller cuts out sterilization cleaning valve II 20, close sampling pump 16, start sampling pond to stir, after time delay T5, controller control waist valve 18 is opened, and sampling pump 16 reverses, and the disinfection cleaning solution in sampling pond 14 is entered in waste liquid bottle by sewer pipe 17.
When fermentation period is very long, when sampling the gap detected, controlling machine allows to manually boot the cleaning to sampling line and sterilization.When performing Manual-cleaning sterilization, controlling machine pilot piping valve 21 is opened and two-way sampling pump 16 starts sampling, disinfection cleaning solution is by disinfection cleaning solution pipeline I 12, the Chang Kaiguan road of sampling valve 2 and purge valve 8, transfer lime 9 arrives in sampling pond 14, after sampling pond conductance type liquid sensor 15 detects cleaning disinfection liquid, controller is blinded off a line valve 21, close two-way sampling pump 16, after time delay T4 second, waist valve 18 opened by controller, control two-way sampling pump 16 to reverse, open pipeline valve 21, open blower pump 7 and purge valve 8, during to setting-up time, cleaning disinfection liquid in sampling pond 14 and sampling line 9 is entered waste liquid bottle through sewer pipe 17, during manual pipeline sterilizing, sampling operation forbidden by controller.
Claims (10)
1. an automatic sampling system for fermentor tank, is characterized in that: comprise
Sampling unit, is connected to fermentor tank (1) through sampling valve (2), for being taken out from fermentor tank (1) by fermented liquid;
Sampling pond (14), its inside is provided with the conductance type liquid sensor (15) for tracer liquid;
Transfer lime (9), its one end by the road valve (21) is connected to sampling pond (14) by two-way sampling pump (16), and the other end is connected to sampling unit through liquid sensor (10);
Waste-solution line (17), its one end is connected to sampling pond (14) through waist valve (18) by two-way sampling pump (16), and the other end is connected to waste liquid bottle;
And sterile air pipeline (6), it is connected on the transfer lime (9) between sampling unit and two-way sampling pump (16), described sterile air pipeline (6) is provided with purge valve (8) and blower pump (7);
Described sampling valve (2), blower pump (7), purge valve (8), liquid sensor (10), conductance type liquid sensor (15), two-way sampling pump (16) and pipeline valve (21) are all connected to controlling machine.
2. the automatic sampling system of fermentor tank according to claim 1, it is characterized in that: described sampling unit is U-tube (3), described transfer lime (9) is connected bottom with U-tube (3) crooked position, also comprise the vapour pipe (4) be connected in U-tube (3), described vapour pipe (4) is provided with steam valve (5), described steam valve (5) is connected to controlling machine.
3. the automatic sampling system of fermentor tank according to claim 1, it is characterized in that: described sampling unit is insert vitrified pipe sampler (11) in fermentor tank (1) or straight tube sampler, also comprise disinfection cleaning solution pipeline I (12), described disinfection cleaning solution pipeline I (12) is connected to transfer lime (9) through sterilization cleaning valve I (13).
4. the automatic sampling system of the fermentor tank according to claim 1 or 2 or 3, is characterized in that: described transfer lime (9) is sebific duct.
5. the automatic sampling system of fermentor tank according to claim 4, it is characterized in that: also comprise disinfection cleaning solution pipeline II (19), described disinfection cleaning solution pipeline II (19) is connected to two-way sampling pump (16) through sterilization cleaning valve II (20), and described sterilization cleaning valve II (20) is connected to controlling machine.
6. realize a control method for the automatic sampling system of fermentor tank in claim 1, it is characterized in that, comprise the steps:
A) sampling valve (2) be connected with fermentor tank (1) of the required sampling of controlling machine control is opened, the pipeline valve (21) controlling this sampling line is opened, control two-way sampling pump (16) to open, the fermentation broth sample in fermentor tank (1) flows to sampling pond (14) by sampling unit through transfer lime (9);
B) when the liquid sensor (10) in transfer lime (9) detects fermentation broth sample, controlling machine starts timing, after controlling machine time delay T1 second, control sampling valve (2) to close, control blower pump (7) and purge valve (8) unlatching, the sample in transfer lime (9) is sent into sampling pond (14) by sterile air pipeline (6) by sterile air simultaneously;
C) when sampling the conductance type liquid sensor (15) in pond (14) and fermentation broth sample being detected, controlling machine controls two-way sampling pump (16) and closes and control waist valve (18) unlatching, the unnecessary fermentation broth sample of transfer lime (9) enters in waste liquid bottle through waste-solution line (17) under the pressure of sterile air, after controlling machine time delay T2 second, control blower pump (7), purge valve (8), waist valve (18) and pipeline valve (21) to close, complete primary sample;
D), after needing repeated sampling or fermentation broth sample process to complete, controlling machine controls waist valve (18) and opens, and controls two-way sampling pump (16) and rotates backward, entered in waste liquid bottle by the fermentation broth sample sampled in pond (14) by waste-solution line.
7. the automatic sampling system control method of fermentor tank according to claim 6, it is characterized in that: every sub-sampling can repeating step a) to steps d), 2-3 time, to get rid of the impact of debris of sampling last time, at execution of step d) after, controlling machine controls sterilization cleaning valve II (20) and opens and two-way sampling pump (16) startup, and thimerosal is entered by disinfection cleaning solution pipeline II (19) and is transported in sampling pond (14), for cleaning sampling pond (14).
8. the automatic sampling system control method of fermentor tank according to claim 6, it is characterized in that: when fermentor tank (11) is for miniature fermenter or small-sized fermentation tank, execution step a) before and sampling terminate rear controlling machine control sampling valve (2), purge valve (8) and blower pump (7) unlatching, by fermentor tank (1) side stopple coupon in fermentation broth sample be oppositely recycled in fermentor tank (1).
9. the automatic sampling system control method of the fermentor tank according to claim 6 or 7 or 8, it is characterized in that: when sampling unit is U-tube, before execution step a), controlling machine controls sampling valve (2), blower pump (7) and purge valve (8) and opens, time delay T3 performs step a) after second again, and step a) middle sampling valve (2) is no longer opened; When having repeated steps d) time, the steam valve (5) that controlling machine controls on vapour pipe (4) is opened, and control blower pump (7) and purge valve (8) simultaneously and open, after time delay T4 second, controlling machine controls steam valve (5), blower pump (7) and purge valve (8) and closes.
10. the automatic sampling system control method of the fermentor tank according to claim 6 or 7 or 8, it is characterized in that: when sampling the gap detected, controlling machine allows to manually boot the clear pond to sampling line and sterilization, controlling machine pilot piping valve (21) is opened and two-way sampling pump (16) starts sampling, disinfection cleaning solution is by disinfection cleaning solution pipeline I (12), the Chang Kaiguan road of sampling valve (2) and purge valve (8), transfer lime (9) arrives in sampling pond (14), after sampling pond conductance type liquid sensor (15) detects cleaning disinfection liquid, controller is blinded off a line valve (21), close two-way sampling pump (16), after time delay T4 second, waist valve (18) opened by controller, control two-way sampling pump (16) reversion, open pipeline valve (21), open blower pump (7) and purge valve (8), during to setting-up time, cleaning disinfection liquid in sampling pond (14) and sampling line (9) is entered waste liquid bottle through sewer pipe (17).
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| CN112695067A (en) * | 2021-01-27 | 2021-04-23 | 泗阳华盛生物科技有限公司 | Tapping sampling and detection method for liquid fermentation tank strain |
| CN113267383A (en) * | 2021-06-18 | 2021-08-17 | 江苏丰泽生物工程设备制造有限公司 | Fully-closed automatic sampling device capable of intelligently distinguishing waste liquid and using method thereof |
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