CN104840370B - Additive and cosmetics used for cosmetic - Google Patents

Abstract

The invention discloses a kind of additive used for cosmetic and cosmetics, additive used for cosmetic be containing α-D- glucopyranose -1,6- D-sorbite with sugar alcohol obtained by α-D- glucopyranose -1, the equimolar mixture of 1- mannitol.Cosmetics contain additive used for cosmetic.There is the present invention efficient hydroxy radical to eliminate active function, while disclose its efficient anti-inflammatory effect.

Description

Additive and cosmetics used for cosmetic

Technical field

The present invention relates to a kind of additive and cosmetics, more particularly to a kind of additive used for cosmetic and cosmetics.

Background technique

Many glycitols such as glycerol, antierythrite, xylitol, mannitol, D-sorbite, trehalose are in cosmetic field It is widely used for moisturizer.Especially antierythrite, xylitol, mannitol, D-sorbite, certainly because of cancellation hydroxyl with higher It is known by the active function of base, has prevention well because of ultraviolet light using the cosmetics containing such glycitols raw material Etc. reasons, because of the effect for various problems such as hair caused by by hydroxy radical is impaired, cutis laxa and wrinkles.

However, having been used mostly in many cosmetics such as hair washing product, shaving cream, foundation emulsion as emulsifier, foaming Agent, remover a kind of Surfactant SDS (referred to as: SDS), and it is well known that SDS is to easily cause skin The inflammation of skin.In addition to this, along with the activity for eliminating hydroxy radical, many cosmetics producers start to be dedicated to researching and developing Inhibit the higher cosmetic material of the inflammatory effect as caused by SDS.

Meanwhile one kind as sugar alcohol, α-D- glucopyranose -1,6- D-sorbite (referred to as: GPS) and α-D- pyrans Portugal The sugar alcohol that grape -1 and 1- of sugar mannitol (referred to as: GPM) equimolar mixture is formed, becomes trade name " different malt " (three wells The registered trademark of sugaring Co., Ltd.).However, different malt is being intended only as sweetener, anti-blushing agent, incremental agent, excipient in the past Use, and for its utilization and performance (hydroxy radical eliminates activity, anti-inflammatory effect etc.) as cosmetic material in terms of Research be missing from.

Summary of the invention

Technical problem to be solved by the invention is to provide a kind of additive used for cosmetic and cosmetics, primarily to Can be allowed to as cosmetic material come using glycitols raw material there is same hydroxy radical to eliminate except active function, Its efficient anti-inflammatory effect is disclosed simultaneously, using quality-high and inexpensive outstanding sugar alcohol as cosmetic additive agent, and provides and contains The cosmetics of the cosmetic material of the substance.

The present invention is to solve above-mentioned technical problem by following technical proposals: a kind of additive used for cosmetic, special Sign is, for containing α-D- glucopyranose -1,6- D-sorbite with α-D- glucopyranose -1,1- mannitol equimolar Mixture obtained by sugar alcohol.

The present invention also proposes a kind of cosmetics, which is characterized in that the cosmetics contain additive used for cosmetic, the cosmetics With additive be containing α-D- glucopyranose -1,6- D-sorbite with α-D- glucopyranose -1,1- mannitol equimolar Mixture obtained by sugar alcohol.

The positive effect of the present invention is that: sugar alcohol contained in additive used for cosmetic of the invention, with conduct There is other sugar alcohols that cosmetic material is utilized same hydroxy radical to eliminate activity, in addition to this, be demonstrated by it to SDS's High anti-inflammatory effect, also, when the Hair grooming hair dryer crossed by its aqueous solution soaking is dry, it can be very good to prevent high temperature correct Deliver the destruction of cortex construction.In addition, similar to other carbohydrates, different malt is cheap, can be bought with lower cost.Cause This, additive used for cosmetic of the invention, as have eliminate hydroxy radical activity, inhibit SDS cause inflammation anti-inflammatory effect, And the outstanding cosmetic material of hair epidermis structure destruction effect caused by high temperature is prevented, it can be effectively utilized.And And cosmetics of the invention can be very good to prevent UV-induced due to containing suchlike additive used for cosmetic The problems such as hair is impaired, cutis laxa, wrinkle, while the pachylosis as caused by SDS and skin red dot inflammation and by drying The control efficiency of hair epidermis structure destruction caused by machine high temperature is significant, while inexpensive can produce.

Detailed description of the invention

Fig. 1 is the schematic diagram for indicating the result of experimental example 1.

Fluorescence microscope of the Fig. 2 for the hair that was impregnated using the basal liquid of experimental example 2 after UVA and UVB irradiation The schematic diagram of photo.

Fig. 3 be the hair that impregnated of 0.1% isomalt (isomaltulose) solution of experimental example 2 through UVA and The schematic diagram of fluorescent microscopy images after UVB irradiation.

Fig. 4 is fluorescence microscopy of the hair after UVA and UVB irradiation after 0.1% different malt solution of experimental example 2 is impregnated The schematic diagram of mirror photo.

Fig. 5 is schematic diagram of the experimental example 3 without the microscope photo of the UV hair (base-material) irradiated.

Fig. 6 is electron micrograph of the hair crossed of 1% different malt aqueous solution soaking of experimental example 3 after UV irradiates Schematic diagram.

Fig. 7 is the schematic diagram of electron micrograph of the hair that was soaked in water of experimental example 3 after UV irradiates.

Fig. 8 is the schematic diagram for the electron micrograph that the hair that hair dryer high temperature drying is crossed is not used in experimental example 4.

Fig. 9 is the electron micrograph after the heated-air drying for the hair that 1% different malt aqueous solution soaking of experimental example 4 is crossed Schematic diagram.

Figure 10 is the schematic diagram of electron micrograph of the hair that impregnated of water of experimental example 4 after heated-air drying.

Specific embodiment

Hereinafter, specific implementation form is illustrated according to the present invention.But the present invention is not limited to the explanation, not In departing from the scope of the present invention, according to the knowledge of dealer, after making various changes, correct and improveing obtained by result.

The present invention is to reach object above, the work about the glycitols as cosmetic material that do not studied up to now With and effect (hydroxy radical eliminates activity, anti-inflammatory effect etc.), strictly study its it is both effectiveness while, with different malt (palatinit) sugar alcohol known for trade name (registered trademark of Shin Mitsui Sugar Co. Ltd), be not only found to have with The same hydroxy radical of antierythrite, xylitol, mannitol, D-sorbite eliminates activity, and has the high anti-inflammatory to SDS Effect, more has found its aqueous solution, after impregnating hair, can be effectively prevented hot wind enemy in heated-air drying and deliver cortex The destruction of construction.

The present invention is to be accomplished by inventor according to the new function (functionality as cosmetic material) of different malt. That is, additive used for cosmetic of the invention be containing α-D- glucopyranose -1,6- D-sorbite with α-D- glucopyranose - 1, sugar alcohol obtained by the equimolar mixture of 1- mannitol contains the additive used for cosmetic of different malt.That is, this hair Bright cosmetics are the cosmetics containing additive used for cosmetic.

The sugar alcohol contained in additive used for cosmetic of the invention, the different malt (Shin Mitsui Sugar Co. Ltd of trade name Registered trademark) be widely known, be that two class of compound of glucose and fructose is sugared (the entitled isomalt of compound), Restore the raw material of (adding hydrogen) origin.Meanwhile the sugar alcohol is referred to as other than different malt, Palatinitol, also referred to as restores different wheat Bud ketose alcohol, isomalt.Lower formula (I) is respectively the chemical formula of isomalt, and lower formula (II) is the chemical formula of different malt.

Chemical formula (I):

Chemical formula (II):

In conclusion different malt is not used for cosmetic material so far, but as described in following experimental example, as makeup Product raw material, antierythrite, xylitol, mannitol, D-sorbite studied and find and be widely used have equivalent effect Hydroxyl radical free radical eliminate activity.

Furthermore different malt is confirmed as shown in following experimental example with the high anti-inflammatory effect for SDS.Meanwhile by SDS Caused by inflammation, especially because seborrhea caused by its surface-active effect, horn cell substance (lipid, NFM etc.) Outflow, between horn cell the reasons such as destruction of film and caused by skin sparing hypofunction, generated because of its repair process Cell factor (IL-2, TFN- α etc.) causes.Although not learnt also at present about approach of the different malt to SDS anti-inflammatory effect, Do not heard that other few carbohydrates had the report of similar anti-inflammatory effect originally yet.Research through the invention learns, xylitol And antierythrite is currently without the anti-inflammatory effect being found as different malt.

In addition, after the aqueous solution soaking hair of different malt, it is dry that hair dryer can be effectively prevented as shown in following experimental example High temperature enemy delivers the destruction of cortex construction when dry hair.

Also, different malt can be purchased as other carbohydrates with lower cost.

It to the discovery of different malt effect and is applied flexibly by the inventor, additive used for cosmetic of the invention can be used as The cosmetic material for manufacturing outstanding cosmetics gives full play to and eliminates active function using its active acid element, to as caused by SDS The effects of destruction that enemy delivers cortex construction when inflammation anti-inflammatory effect, hair dryer high temperature drying hair prevents effect.

Meanwhile cosmetics of the invention are generated because containing additive used for cosmetic of the invention, therefore for ultraviolet light etc. Free radical caused by the damage of hair, the relaxation of skin, wrinkle etc. preventive effect highly significant, and skin caused by SDS Hair epidermis structure destruction caused by skin is coarse and the inhibition of inflammation of skin red dot etc., hair dryer high temperature prevents effect Equal highly significants, while inexpensive can produce.

It specifically include perfumed soap, face cleaning product, face cream, lotion, toner, Cologne as cosmetics according to the present invention Water picks palpus frost, picks palpus water, make-up base Essence, sun cream, sunscreen, solar oil, sunblock lotion, whitening powder, foundation cream, perfume, powder Cake, nail polish, unloads first water, eyebrow pencil, blush, eye cream, eye shadow, mascara, eyeliner, lipstick, lipstick at manicure cream, including Bath apply some make up equal skin nursing products, shampoo, hair conditioner, hair dye and hair user using product hair shield Reason material, the simultaneously products such as including toothpaste.

The additive used for cosmetic of the invention can according to need other than different malt containing other compositions.In addition, Cosmetics of the invention, according to its specific type and purposes, can also containing other than invention additive used for cosmetic at Point.

Ingredient other than the different malt that can contain in additive used for cosmetic in the invention and it can be used for the hair Can be used in cosmetics in bright it is noted earlier other than ingredient and nonspecific, for example, being made in general cosmetics Water composition, powder, surfactant, finish, moisturizer, alcohols, PH regulator, preservative, pigment, antioxidant, Tackifier, fragrance etc. can be allowed to independent or plural combination and contain.

In addition, cosmetics in the amount and the present invention of the different malt that can contain in additive used for cosmetic in the present invention The amount of the middle preceding note additive used for cosmetic that can contain is distinguished and is provided according to the type of cosmetics, purposes etc..

The following are the detailed descriptions that the present invention is carried out according to performance, but the present invention is not limited solely to following case Example.

Experimental example 1: active evaluation is eliminated about hydroxy radical

Different malt, 6- fructose-α-glucoside, antierythrite, wood are obtained according to deoxyribose method (Halliwell, 1981) The hydroxy radical elimination factor of sugar alcohol, D-sorbite.

Specifically, 33.6mM- deoxyribose (ultimate density 4.2mM), 200 μ L- oxidation is added first in Purified Water Iron (III)/1.04mM-EDTA solution (Fe ultimate density is 100 μM), 120mM- phosphate buffer (pH7.4, ultimate density 15mM), 1mM- ascorbic acid (150 μM of ultimate density), 0.02% hydrogen peroxide, the thio barbital of 50mM-NaOH, 1%TBA( Acid)/50mM-NaOH solution and 2.8%TCA(trichloroacetic acid), it is modulated into reagent.In the reagent, various glycitols (different malt, Isomalt, antierythrite, xylitol, D-sorbite) debugged according to various concentration shown in table 1 and table 2 made of Solution.Meanwhile glycitols solution is substituted in the reagent using Purified Water, it is used as basic liquid.

In 1.5 milliliters of PP pipe, 100 each strength solutions or comparison liquid are injected, adds 50 on this basis Phosphate buffer, deoxyribose 50, hydrogen peroxide 50, iron chloride (III)/EDTA solution 100, ascorbic acid 50 , finally obtain 400 mixed solutions.When PP tube wall has liquid to be stained with, PP pipe is shaked gently, liquid is allowed to drop into mixed solution. Then, in the sink by PP manage in solution be heated to 37 DEG C, and make its react 1 hour after, from sink take out PP pipe, injection TBA350, TCA350, then by PP pipe be put into sink heating make PP manage in after solution reaches 100 DEG C, placement makes its reaction After twenty minutes, PP pipe is taken out from sink, is put and is cooled down in water.After each solution is cooling in PP pipe, using pure water as titer, survey Fixed absorbance of each solution in wavelength 532nm.Each concentration samples or comparison liquid are subjected to absorbance measurement, take 3 times flat Mean value (n=3), and (1) calculates the elimination factor of hydroxy radical according to the following formula, result looks at lower Fig. 1 and table 1, table 2.

Hydroxy radical elimination factor (%)=[1-(sample absorbance ÷ comparison liquid absorbance)] × 100 ... (1)

Table 1

Table 2

As shown in table 1, table 2 and Fig. 1, being demonstrated by different malt and antierythrite, xylitol, D-sorbite has equal height Hydroxy radical cancellation activity.Separately as shown in Figure 1, being demonstrated by various glycitols concentration and hydroxy radical elimination factor has logarithm Close number curve correlation.Meanwhile although isomalt hydroxy radical elimination factor compared with other glycitols is lower, through testing Speculate this is because only isomalt is reduced sugar in sugar alcohol used in sample.Hydroxy radical about reduced sugar The lower saying of activity is eliminated, is pointed out in the case of No. 2001-26536 patent document of Japanese Unexamined Patent Publication, the red moss of 0.5% concentration Sugar alcohol solution is 64.3% to the inhibition production rate of MDA.

Experimental example 2: about the verifying for generating obstruction effect for hair active oxygen

After hair is irradiated with ultraviolet radiation, the active oxygen that hydrogen peroxide, hydroxy radical etc. easily react is generated, therefore and generate Chain reaction is aoxidized, promotes cystine to turn to the oxidation of cysteic acid, so that the cystine linkage of hair be promoted to be formed, and causes Damage to hair epidermis.The experiment utilizes the active oxygen fluorescence reaction of fluorescein, demonstrates different malt and isomaltoketose The prevention effect that alcohol generates hair active oxygen.Hydroxy phenyl fluorescein (abbreviation HPF) does not have fluorescence in neutral aqueous solution, but It is to generate fluorescent both compounds fluorescein (excitation wavelength after reacting with the oxide that same active oxygen has same high response 490nm, wavelength of fluorescence 515nm), and can observe the increase of fluorescence intensity.HPF can be observed the increasing of fluorescence intensity It greatly, is the result reacted with hydroxyl.

About specific verification method, 100 are put by PBS(Phosphate Buffered Salts) 1, tablet first In ion exchange water, then magnetic stirring apparatus is inserted into the ion exchange water and stirs and modulates into comparison liquid.Then, with compare In the PBS solution 1ml that liquid equally forms, isomalt (sugaring of three wells produces) 1mg is added, is modulated into containing 0.1% different malt The solution of ketose alcohol.Again by 1ml comparison liquid in composition PBS solution, the addition different malt of 1mg (sugaring of three wells produces) is modulated into 0.1% different malt solution.Later in 5mM-HPF (Sekisui Fine Chemical Co., Ltd. makes) solution 1, addition comparison liquid is same to form PBS Solution 1 is modulated into the HPF solution of 100 μm of ol/L.

By above-mentioned modulated comparison liquid and 0.1% isomaltoketose alcoholic solution, 0.1% different malt solution takes respectively 1ml is put into 35mm plate (35mmTissue Culture Dish:IWAKI Co., Ltd. makes).Then, the hair of 1cm will be cut into 10 are soaked respectively and are placed in the comparison liquid/0.1% isomaltoketose alcoholic solution/0.1% different malt solution plate divided just now.And Paying attention to separating in hair not be overlapped.Then UVB and UVB illumination in 10 minutes is carried out from the top of each plate.Later, Cong Gepan Son takes out hair, is placed on glass slide, covers slide after the good HPF solution of 10 aforementioned modulations is dripped on hair, aobvious with fluorescence Micro mirror (NIBA: Olympus Co., Ltd makes (excitation filtering 470-490nm)) is observed and takes photo.Fig. 2 is comparison liquid leaching The schematic diagram of fluorescent microscopy images of the hair under UVA and UVB irradiation after bubble, Fig. 3 is 0.1% isomaltoketose alcoholic solution The schematic diagram of fluorescent microscopy images of the hair under UVA and UVB irradiation after immersion, Fig. 4 are that 0.1% different malt solution is impregnated The schematic diagram of fluorescent microscopy images of the hair afterwards under UVA and UVB irradiation.

As shown in Fig. 2, can in the fluorescent microscopy images that the hair that comparison liquid was impregnated carries out after UVA and UVB irradiation To see fluorescence.This is because hair generates hydroxy radical after ultraviolet light irradiates, the cystine quilt of cystine linkage is formed to hair Cysteic acid aoxidizes and causes the damage of hair epidermis, the hydroxyl generating portion of exposing reacted with HPF after result.Fig. 3 and Fig. 2 It compares, the difference of fluorescence degree is little, it can be seen that, isomalt, which generates the active oxygen of hair, hinders effect weaker. However, compared with Fig. 2, the fluorescent weakening of Fig. 4, therefore visible different malt generates the active oxygen of hair and hinders effect more bright It is aobvious.This is because different malt in conjunction with hydroxy radical, prevents the chain reaction of oxidation.

Experimental example 3: the verifying about the protective effect for preventing hair damages under UV irradiation

By the aqueous solution of water and 1% different malt cooperation, 1ml is taken to pour into the glass tray of 35mm respectively, then will be ready Each 10 of the hair of healthy women between -30 years old 20 years old of 1cm length immerse water and 1% different malt aqueous solution just now respectively In.Hair root root is not overlapped separately.Then, 20 minutes 280-400nm of UV(wavelength are carried out above glass tray) irradiation Afterwards, with the cuticle state of micro- sem observation hair and electron micrograph is shot.Meanwhile object as a comparative reference, do not have The hair for carrying out UV irradiation similarly observes its sink conditions under the microscope and shoots microscope photo.Fig. 5 is without UV The hair microscope photo of irradiation, Fig. 6 are after 1% different malt aqueous solution soaking and to carry out the electron micrograph after UV irradiation, Fig. 7 is that the hair being soaked in water carries out the electron micrograph after UV irradiation.

The hair being soaked in water carries out the state (Fig. 7) of UV irradiation, with the comparison hair condition (figure for not carrying out UV irradiation 5) it is compared observation, learns that UV irradiation has the destruction of wider area construction to hair epidermis, and keep hair more serious impaired. However, state (Fig. 6) of the hair crossed of 1% different malt aqueous solution soaking after UV irradiates, after being compared observation with Fig. 5, discovery It has almost no change, hair epigenetic structure integrally remains good.Therefore, it is learnt from the above Germicidal efficacy, different malt has hair There is anti-UV irradiation to cause impaired protective effect.

Experimental example 4: the verifying about hair dryer high temperature to the protective effect of hair damage

By the aqueous solution of water and 1% different malt cooperation, 1ml is taken to pour into the glass tray of 35mm respectively, then will be ready Each 1 beam of the hair of healthy women (every beam 20) between -30 years old 20 years old of 1cm length, immerses water just now and 1% different wheat respectively In bud aqueous solution.After immersion, the topknot in each tray is taken out, 10 minutes lasting heated-air dryings is carried out to it respectively, blows Blower is away from topknot 3cm.Then with the cuticle state of hair in each topknot of micro- sem observation and electron micrograph is shot.Together When, object, the hair for not carrying out heated-air drying are similarly observed its sink conditions under the microscope and are shot as a comparative reference Microscope photo.Fig. 8 is the hair microscope photo crossed without hair dryer heated-air drying, is spelled as control；Fig. 9 is 1% different wheat The schematic diagram of electron micrograph after carrying out heated-air drying after bud aqueous solution soaking and with hair dryer, Figure 10 are to be soaked in water The schematic diagram of the electron micrograph after machine heated-air drying is blowed in the hair crossed.

The hair being soaked in water carry out heated-air drying with hair dryer after state (Figure 10), and do not carry out heated-air drying Hair (reference substance) state (Fig. 8) is compared observation, learns that hair dryer heated-air drying has wider model to hair epigenetic structure The destruction enclosed, and since the high temperature of hair dryer makes hair have more serious damaged phenomenon.However, 1% different malt aqueous solution soaking State (Fig. 9) of the hair crossed after hair dryer heated-air drying, after being compared observation with Fig. 8 without hot blasting, hair It now has almost no change, hair epigenetic structure integrally remains good.Therefore, it is learnt from the above Germicidal efficacy, hair dryer hot air drying It is dry to phenomenon is damaged caused by hair, different malt has good protective effect.

Experimental example 5: it is directed to the verifying of lauryl sodium sulfate (SDS) anti-inflammatory effect

This experiment utilizes human body patch test, is examined to the SDS anti-inflammatory effect of different malt and isomalt It tests.Patch test is carried out using conventional method (24 hours human body patch).Firstly, using purification pure water as solvent, adjust Produce following several solns: 5%SDS(Wako Pure Chemical Industries Co., Ltd. makes) solution and 5% different malt solution, the different wheat of 5%SDS and 1% Bud solution, 5%SDS and 5% isomaltoketose alcoholic solution and 5%SDSD solution.

Next, having each of the patch device (registered trademark of Smartpractice company) in 5 holes diameter 8mm in design On a hole, the patch scraps of paper that installation diameter is 8mm are smeared aforementioned four kinds of modulated solution on each scraps of paper and are refined pure Water.Then, four men and women that this patch device sets the age between 30~40 years old are tested into volunteer's forearm and bends side, sticked 24 hours. Later, patch device is removed from volunteer's forearm, after placing 30 minutes, determines each solution to volunteer's skin by visually observing Caused by the inflammatory conditions such as coarse, red phenomenon.And go out three results according to following standard determination.

Criterion: (++) ... with reference material it was found that, degree of inflammation is substantially reduced；

(+) ... with reference material it was found that, degree of inflammation slightly mitigates；

(-) ... has the inflammatory phenomena of equal extent with reference material.

Table 3

Result is learnt as shown in Table 3, and different malt and isomalt all have inhibiting effect to SDS inflammatory phenomena, still In the identical situation of solution concentration, different malt solution obviously has higher anti-inflammatory effect.

The present invention is used as the raw material of the various products such as various cosmetics and skin nursing products, hair-washing hair-care, can be very It is used for additive used for cosmetic well.

Claims (1)

1. a kind of additive used for cosmetic is to contain α-D- pyrans Portugal preparing the purposes in cosmetics, the cosmetic additive agent Grape sugar -1,6- D-sorbite with sugar alcohol obtained by α-D- glucopyranose -1, the equimolar mixture of 1- mannitol, feature It is,

The sugar alcohol is applied in preparing cosmetics, and the cosmetics prepared at least have the spy of (3) in following (1)-(3) Sign,

(1) with other sugar alcohols being utilized as cosmetic material there is same hydroxy radical to eliminate activity；

(2) there is the high anti-inflammatory effect to SDS；

(3) have the function of preventing hair epidermis structure destruction caused by high temperature.