CN104792782A - Acetylcholine esterase activity detection test paper strip based on silver nanoparticles - Google Patents
Acetylcholine esterase activity detection test paper strip based on silver nanoparticles Download PDFInfo
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- CN104792782A CN104792782A CN201510219755.5A CN201510219755A CN104792782A CN 104792782 A CN104792782 A CN 104792782A CN 201510219755 A CN201510219755 A CN 201510219755A CN 104792782 A CN104792782 A CN 104792782A
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Abstract
The invention relates to a cholinesterase activity detection test paper strip based on silver nanoparticles. The test paper strip comprises a paper board, wherein water absorbent paper, a detection pad, a reduction pad and a sample pad are sequentially adhered on one side of the paper board from top to bottom, and the adjacent pads are overlapped and connected at the joints; the detection pad is a cellulose nitrate film, three detection lines are arranged on the film from top to bottom, and the detection lines are respectively coated with silver nitrate water solutions with concentrations from high to low; and the reduction pad is glass fiber and is sprayed with a certain concentration of HEPES solution of vitamin C. The test paper strip can be used for rapid detection of activity of cholinesterase and has the advantages of simplicity and convenience in use, high sensitivity, capability of obtaining detection results without relying on analysis of instruments and the like. The detection sensitivity of the established detection test paper strip against active cholinesterase can achieve 0.01nM, so that a new method can be provided for detection of the activity of cholinesterase.
Description
Technical field
The present invention relates to a kind of cholinesterase activity detection side based on silver nano-grain to chromatograph test strip.
Background technology
At present, a large amount of uses of chemical pesticide, particularly organophosphorus pesticide have larger threat to the healthy of people and Environmental security, therefore have larger social effect to the highly sensitive detection of organophosphorus pesticide.
Organophosphorus insecticide is of a great variety, current detection method, particularly method for quick be difficult to realize organophosphorus pesticide polycomponent how residual while detect.Research finds, organophosphorus pesticide is the compound that a class can reduce cholinesterase activity, and therefore people utilize the change of cholinesterase activity can detect the residual quantity of organophosphorus pesticide in agricultural product.As used many Ellman colourimetrys at present.But the method sensitivity is lower, and there is certain dependence to instrument, therefore set up a kind of easy and simple to handle, quick, highly sensitive, to organophosphorus pesticide polycomponent, many residual quick detections, there is larger meaning without the cholinesterase activity detection method relied on to instrument.
The invention provides a kind of cholinesterase activity detection side based on silver nano-grain to chromatograph test strip.This test strips comprises cardboard, and the one side of cardboard pastes thieving paper, detecting pad, reduction pad and sample pad from top to bottom successively, the overlapping connection in junction of adjacent each pad.Wherein detecting pad is nitrocellulose filter, and this film is provided with three detection lines from top to bottom, and bag is by concentration silver nitrate aqueous solution from high to low respectively; Reduction pad is glass fibre, is coated with certain density ascorbic HEPES solution.This test strips can be used for the quick detection of cholinesterase activity, has easy to use, highly sensitive, and testing result is without the need to relying on the advantages such as instrumental analysis.The test strip set up, can reach 0.01nM to active choline esterase detection sensitivity, can be cholinesterase activity detection and provides new method.
Summary of the invention
The object of this invention is to provide a kind of lateral chromatography test strips that can be used for rapid semi-quantitative detection cholinesterase activity.
Object of the present invention realizes by following technical scheme:
A kind of cholinesterase activity test strip based on silver nano-grain.This test strips comprises cardboard, and the one side of cardboard pastes thieving paper, detecting pad, reduction pad and sample pad from top to bottom successively, the overlapping connection in junction of adjacent each pad.Wherein detecting pad is nitrocellulose filter, and this film is provided with three detection lines from top to bottom, and bag is by concentration silver nitrate aqueous solution from high to low respectively; Reduction pad is glass fibre, is coated with the ascorbic HEPES solution that concentration is 0.08M.
Liner plate in described test strips is the toughness material do not absorbed water, for hard plastic bar or the cardboard bar that do not absorb water are made.
Described reduction pad and sample pad are glass fibre element film, are made up of glass fibre silk barathea.
Described thieving paper is the absorbent filter or filter paper for oil that water-intake capacity is stronger.
Described cellulose membrane is nitrocellulose filter.
Beneficial effect
The present invention is in conjunction with the advantage of lateral chromatography test strips and nano material, and that sets up is highly sensitive, easy to carry for the lateral chromatography test strips detecting cholinesterase activity fast, and testing result can not rely on instrument.This ELISA test strip sensitivity can reach 0.01nM.
Embodiment
1. for detecting the assembling of the lateral chromatography test strips of cholinesterase activity
The invention provides a kind of cholinesterase activity test strip based on silver nano-grain.This test strips comprises cardboard, and the one side of cardboard pastes thieving paper, detecting pad, reduction pad and sample pad from top to bottom successively, the overlapping connection in junction of adjacent each pad.Wherein detecting pad is nitrocellulose filter, and this film is provided with three detection lines from top to bottom, and bag is the silver nitrate aqueous solution of 0.5,0.3,0.3nM by concentration respectively, Bao Beihou, under detecting pad lucifuge condition, and 37 DEG C of vacuum drying 1-2 hour; Reduction pad is glass fibre, is coated with the ascorbic HEPES solution that concentration is 0.1M, after spraying, and reduction pad 37 DEG C of vacuum drying 1 hour.Thieving paper is the absorbent filter or filter paper for oil that water-intake capacity is stronger, and sample pad is glass fibre plastic film, and thieving paper and sample pad are assembled front without the need to special processing.
Before and after the assembling of this test strips, answer lucifuge, kept dry.
2. the detection of acetylcholine esterase active
Acetylcholinesterase is made into the HEPES solution of gradient concentration, concentration is respectively: 0.01,0.02,0.05,0.1,0.2,0.5,1.0,2.0,5.0,10.0nM, mixes successively with BuCh (BTcH) equal-volume of 0.5mg/mL.Mixed liquor reacts 15 minutes at 37 DEG C, gets each concentration-response liquid 120uL respectively and carries out lateral chromatography test.Wherein, the HEPES solution of the BTcH of comparable sodium is set as blank.
Color on detection line, after 7 minutes, is compared: in blank by chromatography, because do not have the catalysis of cholinesterase, in test strips, three detection lines all demonstrate obvious yellow (color of silver nano-grain); Along with the raising of cholinesterase concentration, improve in solution to the catalytic capability of BuCh, in chromatographic solution, thiol concentration raises, and then reduces intensity yellow on detection line.According to three detection lines color disappears completely, develop the color three intensity that are more weak and that develop the color completely, the sensing range of cholinesterase is divided into: < 0.01nM, (0.01,0.02], (0.02,0.05], (0.05,0.1], (0.1,1.0], and 6 concentration ranges such as > 1.0nM, and then realize detecting the rapid semi-quantitative of cholinesterase activity.
Claims (3)
1. based on a cholinesterase activity test strip for silver nano-grain, it is characterized in that: it comprises cardboard, the one side of cardboard pastes thieving paper, detecting pad, reduction pad, sample pad from top to bottom successively, the overlapping connection in junction of adjacent each pad; Described detecting pad is nitrocellulose filter, and this film arranges three detection lines from top to bottom, wraps by concentration silver nitrate aqueous solution from high to low respectively; Described reduction pad is glass fibre, is coated with ascorbic HEPES solution.
2. a kind of cholinesterase activity test strip based on silver nano-grain described in claim 1, is characterized by: it is the silver nitrate aqueous solution of 0.5,0.3,0.3nM that described three detection lines wrap by concentration respectively.
3. a kind of cholinesterase activity test strip based on silver nano-grain described in claim 1, is characterized by: described reduction pad upper spraying concentration is the ascorbic HEPES solution of 0.08M.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510219755.5A CN104792782A (en) | 2015-04-28 | 2015-04-28 | Acetylcholine esterase activity detection test paper strip based on silver nanoparticles |
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| CN201510219755.5A CN104792782A (en) | 2015-04-28 | 2015-04-28 | Acetylcholine esterase activity detection test paper strip based on silver nanoparticles |
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| CN104792782A true CN104792782A (en) | 2015-07-22 |
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| CN201510219755.5A Pending CN104792782A (en) | 2015-04-28 | 2015-04-28 | Acetylcholine esterase activity detection test paper strip based on silver nanoparticles |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107727861A (en) * | 2017-08-22 | 2018-02-23 | 厦门依柯利斯医疗科技有限公司 | A kind of pepsin measure kit and assay method |
| CN109060790A (en) * | 2018-09-06 | 2018-12-21 | 吉林大学 | Acetylcholine esterase active test strip and preparation method thereof based on hydroxy cobalt oxide nanometer sheet |
| WO2019024148A1 (en) * | 2017-07-31 | 2019-02-07 | 南京医科大学第一附属医院 | Colloidal gold chromatography rapid diagnostic test strip for detecting acetylcholinesterase and preparation method therefor |
| CN109975374A (en) * | 2019-03-18 | 2019-07-05 | 浙江大学 | Composite conducting slurry and electrode preparation method based on conducting polymer and silver nano-grain and the application in detection of organic phosphorus pesticide |
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2015
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| US20090111171A1 (en) * | 2003-02-13 | 2009-04-30 | Inverness Medical Switzerland Gmbh | Lateral flow immunoassay controls |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019024148A1 (en) * | 2017-07-31 | 2019-02-07 | 南京医科大学第一附属医院 | Colloidal gold chromatography rapid diagnostic test strip for detecting acetylcholinesterase and preparation method therefor |
| CN107727861A (en) * | 2017-08-22 | 2018-02-23 | 厦门依柯利斯医疗科技有限公司 | A kind of pepsin measure kit and assay method |
| CN107727861B (en) * | 2017-08-22 | 2019-10-22 | 厦门依柯利斯医疗科技有限公司 | A kind of pepsin assay kit and measuring method |
| CN109060790A (en) * | 2018-09-06 | 2018-12-21 | 吉林大学 | Acetylcholine esterase active test strip and preparation method thereof based on hydroxy cobalt oxide nanometer sheet |
| CN109060790B (en) * | 2018-09-06 | 2020-11-10 | 吉林大学 | Acetylcholinesterase activity detection test paper strip based on cobalt oxyhydroxide nanosheet and preparation method thereof |
| CN109975374A (en) * | 2019-03-18 | 2019-07-05 | 浙江大学 | Composite conducting slurry and electrode preparation method based on conducting polymer and silver nano-grain and the application in detection of organic phosphorus pesticide |
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Application publication date: 20150722 |