CN104673814B - A kind of L threonine aldolases for coming from enterobacter cloacae and its application - Google Patents
A kind of L threonine aldolases for coming from enterobacter cloacae and its application Download PDFInfo
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- CN104673814B CN104673814B CN201510089583.4A CN201510089583A CN104673814B CN 104673814 B CN104673814 B CN 104673814B CN 201510089583 A CN201510089583 A CN 201510089583A CN 104673814 B CN104673814 B CN 104673814B
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- nucleotide sequence
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- enterobacter cloacae
- threonine aldolase
- aldolase
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Abstract
The present invention relates to a kind of nucleotide sequence of the encoding L-threonine aldolase separated from enterobacter cloacae, and it is with SEQ ID NO:The fragment of nucleotide sequence or the nucleotide sequence shown in 1, analogs and derivatives.The nucleotide sequence that the present invention includes encoding L-threonine aldolase is connected with exogenous regulatory sequence, carries out the filial generation of the carrier of functional expression and the multicellular organism containing carrier of the present invention and this kind of organism.The method for preparing L β hydroxyls alpha amino acids or D β hydroxyl alpha amino acids with the filial generation of above-mentioned nucleotide sequence or peptide sequence or the multicellular organism containing carrier of the present invention and this kind of organism.
Description
Technical field
The invention belongs to field of chemical engineering and enzyme engineering field, is related to one kind and comes from enterobacter cloacae
The L-threonine aldolase of (Enterobacter cloacae tzyx2), it is especially the L-threonine aldehyde contracting of enterobacter cloacae
The nucleotide sequence of enzyme and its application.
Background technology
Aldolase is a kind of reversible lyase, is recognized first by the mankind in 1934.Sour contracting enzyme can be catalyzed confession
Body (nucleopilic reagent, typically ketone) and acceptor aldehyde (electrophilic reagent) carry out reversible aldol reaction.Most of acid contracting enzymes
Donor for them is very single-minded, and possesses the larger free degree for acceptor aldehyde.It is special to donor according to aldolase
The difference of one property, aldolase can be divided into five major classes：Phosphoric acid dihydroxyacetone (DHA) (DHAP) dependent form aldolase, pyruvic acid and phosphoric acid are dilute
Alcohol of formula pyruvic acid dependent form aldolase, acetaldehyde dependent form aldolase, glycine dependent form aldolase and dihydroxy benzylacetone according to
Rely type aldolase.
Threonine aldolase (Threonine Aldolase, TA；EC4.1.2.5 glycine dependent form aldolase) is belonged to,
The characteristics of the type aldolase maximum is in confactor phosphopyridoxal pyridoxal phosphate (Pyridoxal phosphate；PLP presence)
Under, beta-hydroxy-alpha-amino acid can be generated after reaction using amino acid as donor.Threonine aldolase catalysis threonine is cracked into
Glycine and acetaldehyde, it can also be catalyzed glycine under certain condition and acetaldehyde synthesizes threonine.Research shows that the enzyme is for it
Aldehyde acceptor often shows very extensive substrate tolerance, including various substituted aromatic aldehydes and fatty aldehyde, therefore, in recent years
Threonine aldolase starts to attract attention, and is attempted to expand application of the enzyme in synthesis, promotes chemistry and the knot of enzyme engineering
Close.
Be catalyzed in threonine aldolase in the beta-hydroxy-alpha-amino acid of generation, including vancomycin, cyclosporin A and
The key precursor of many antibiotic such as polyoxin D and immunodepressant.In chemical synthesis process, the type beta-hydroxy-α-
The synthesis of amino acid needs multi-step process to separate isomers, and complex process, cost is high, and environmental pollution is serious.And utilize threonine
Aldolase can specifically catalyze and synthesize the different beta-hydroxy a-amino acids of particular configuration, and it does not need any protection group, not yet
Any unnecessary step is needed, does not also produce accessory substance, undoubtedly synthesis beta-hydroxy a-amino acid is most direct, most attracting side
Formula.
According to the stereocpecificity of alpha-carbon atom on the threonine to being acted on, the enzyme can be divided into L-type and the class of D types two.L-
Threonine aldolase (L-TA), can be as the resolving agent of synthesis D- β hydroxyl alpha amino acids.Can also using glycine and aldehydes as
The step enzyme method of substrate one synthesizes L- beta-hydroxy a-amino acids.
The content of the invention
It is an object of the present invention to provide the nucleotides of the encoding L-threonine aldolase separated from enterobacter cloacae
The sequence or fragment of its nucleotide sequence, analog or derivative.From a kind of shaft-like gramnegative bacterium-enterobacter cloacae
The L-threonine aldolase gene of clone in (Enterobacter cloacae tzyx2), by the gene and different expression vectors
Connection, is transferred in bacterium, yeast, plant or animal, and L- beta-hydroxies are produced or split using its encoding L-threonine aldolase
The methods and applications of a-amino acid.
It is a further object to provide the L-threonine aldolase polypeptide coded by the nucleotide sequence or its piece
Section, analog or derivative.
It is a further object to provide being connected containing the gene nucleotide series with heterologous regulatory sequence, work(is carried out
The recombinant vector of energy property expression.
It is a further object to provide one kind containing the gene nucleotide series or the gene nucleotide series with
The recombinant vector conversion of heterologous regulatory sequence connection or host cell and its offspring of transduction.
Used it is a further object to provide one kind and contain the gene nucleotide series or the gene nucleotide series
The recombinant vector conversion being connected with heterologous regulatory sequence or the host cell and its progeny cell of transduction, or the nucleotide sequence institute
The method that the L-threonine aldolase polypeptide of coding produces or splits L- beta-hydroxy a-amino acids.
The first aspect of the present invention, there is provided be with SEQ ID NO:Nucleotide sequence or the nucleotides sequence shown in 1
The fragment of row.The nucleotide sequence of the L-threonine aldolase gene of separation, it includes a nucleotide sequence, the nucleotide sequence
It is identical with a kind of nucleotide sequence being selected from the group：(1) there is coding SEQ ID NO:The core of the active peptides of 2 amino acid sequences
Nucleotide sequence；(2) nucleotide sequence complementary with nucleotide sequence (1), or the foregoing nucleotides sequence for having at least 65% phase same sex
Row.
In the second aspect of the present invention, there is provided the polypeptide coded by this nucleotide sequence of separation, the polypeptide include：Tool
There are SEQ ID NO:The polypeptide of 2 amino acid sequences or its fragment.
The third aspect of the present invention, there is provided the plasmid expression vector containing above-mentioned nucleotide sequence, and by above-mentioned core
Nucleotide sequence or the host cell and its progeny cell of plasmid expression vector conversion.
The fourth aspect of the present invention, there is provided one kind with containing the gene nucleotide series or the gene nucleotide series with
The plasmid expression vector conversion of heterologous regulatory sequence connection or transduction host cell and its progeny cell or with the nucleotide sequence
The method that coded L-threonine aldolase polypeptide produces or splits L- beta-hydroxy a-amino acids.
Other aspects of the present invention are apparent to those skilled in the art due to the disclosure of this paper technologies
's.
As used in the present invention, " separation " refer to material separated from its primal environment (if crude,
Primal environment is natural surroundings).For example, the nucleotide sequence and polypeptide under native state in active somatic cell are not separate
Purifying, but same nucleotide sequence or polypeptide such as from native state with being separated with existing in other materials, then to divide
From purifying.
As used herein, " nucleotide sequence of separation " refers to substantially free of natural relative other albumen, fat
Class, carbohydrate or other materials.Those skilled in the art can use the DNA purification techniques of standard.
The invention provides a kind of nucleotide sequence of new separation --- coding enterobacter cloacae Enterobacter
The nucleotide sequence of cloacae tzyx2 L-threonine aldolase, it is substantially by SEQ ID NO:Nucleosides shown in 1
Acid sequence composition, it is characterized in that：The a length of 1002bp of the sequence (base), it is encoding L-threonine aldolase mature polypeptide SEQ
ID NO:2 ORFs.
The invention provides the nucleotide sequence of separation, the nucleotide sequence has SEQ ID NO by coding:2 amino acid
The nucleotide sequence composition of the active peptides of sequence.Specifically, nucleotide sequence of the invention has SEQ ID NO:1 nucleosides
Acid sequence.Encode SEQ ID NO:The nucleotide sequence of 2 active peptides includes：The only coded sequence of mature polypeptide；It is ripe more
The coded sequence of peptide and various additional coding sequences；The coded sequence (and optional additional coding sequence) of mature polypeptide and non-
Coded sequence.
Polypeptide and nucleotide sequence in the present invention preferably provide in a separate form, are more preferably purified to homogeneous.
Specific nucleotide sequence can be obtained with a variety of methods in the present invention.For example, divided with hybridization technique well known in the art
Freestone nucleotide sequence.These technologies include but is not limited to：(1) it is homologous to detect with probe and genome or cDNA library hybridization
Nucleotide sequence；(2) antibody screening of expression library is to detect the nucleotide sequence of the clone with structural features
Fragment.
The sequence dna fragment of the present invention can also be obtained with following method：(1) double chain DNA sequence is separated from genomic DNA；
(2) chemical synthesising DNA sequence is to obtain the double-stranded DNA of the polypeptide.
Present invention also offers a kind of new peptide sequence, the amino acid sequence of enterobacter cloacae L-threonine aldolase,
It is by SEQ ID NO:Amino acid sequence composition shown in 2.The polypeptide of the present invention can be recombinant polypeptide, natural polypeptides, synthesis
Polypeptide, preferably recombinant polypeptide.The polypeptide of the present invention can be native purified product, or the product of chemical synthesis, or use
Recombinant technique is from protokaryon or eucaryon host (for example, being produced in bacterium, yeast, higher plant, insect and mammalian cell.
The enterobacter cloacae L-threonine aldolase containing coding can be built with method well-known to those having ordinary skill in the art
The expression vector of nucleotide sequence and suitable transcription/translational control element.These methods include recombinant DNA technology in vi, DNA
[Sambroook, et al., Molecular Cloning, a Laboratory such as synthetic technology, In vivo recombination technology
Manual,Cold Spring Harbor Laboratory,New York,1989].Described coding enterobacter cloacae L- Soviet Unions
The nucleotide sequence of propylhomoserin aldolase can be effectively connected in the appropriate promoter of expression vector, to instruct mRNA to synthesize.
The invention further relates to the weight with the nucleotide sequence that enterobacter cloacae L-threonine aldolase is encoded containing the present invention
Group carrier is directly thin through host caused by genetic engineering with the nucleotide sequence for encoding enterobacter cloacae L-threonine aldolase
Born of the same parents.In the present invention, the nucleotide sequence of enterobacter cloacae L-threonine aldolase or the restructuring containing the nucleotide sequence are encoded
Carrier can be transformed or transduced into host cell, thin to form the genetically engineered host containing the nucleotide sequence or recombinant vector
Born of the same parents.
With nucleotide sequence of the present invention or the conversion of the recombinant vector containing nucleotide sequence host cell can use this
Method known to the technical staff in field is carried out.When host is prokaryotes such as Escherichia coli, the competence that can absorb DNA is thin
Born of the same parents can collect thalline in exponential phase of growth, use CaCl2Method processing, step used are well known in the art.Also can use
MgCl2, carry out the methods of electroporation.When host is eucaryote, DNA infection protocols, microinjection, electroporation, lipid can be selected
The methods of body is packed.
The invention further relates to being produced with above-mentioned genetically modified host cell, according to host cell, with those skilled in the art institute
Known method growth or culture.For example microbial cell is typically preferably 10-60 DEG C at 0-100 DEG C, while also want oxygen.
Contain carbon source, such as glucose in culture medium, the form of nitrogen source, typically organic nitrogen, such as yeast extract, amino acid, or salt, such as
Ammonium sulfate, trace element, such as iron, magnesium salts, also vitamin if desired.The pH of culture medium can keep solid during this period
Fixed value, that is, be controlled or do not control in the training period.Culture can be with batch culture, half discontinuous culture or continuous
Culture form is carried out.After culturing, cell is collected, smashs to pieces or directly uses, pass through method well known to those skilled in the art
L-threonine aldolase is extracted from cell.
The invention further relates to the method that L- beta-hydroxy a-amino acids are produced using L-threonine aldolase polypeptide, this method is
It is achieved in that, by glycine and aldehydes and SEQ ID NO:2 cultivate together.Under certain environmental conditions, preferably temperature
10-50 DEG C, pH6-10, corresponding L- beta-hydroxies a-amino acid (as shown in Figure 1) can be catalyzed and synthesized.
The invention further relates to the method that L- beta-hydroxy a-amino acids are split using L-threonine aldolase polypeptide, this method is
It is achieved in that, DL- beta-hydroxies a-amino acid and SEQ ID NO:2 cultivate together.Under certain environmental conditions, it is preferably temperature
10-50 DEG C, pH6-10 of degree, L- beta-hydroxy a-amino acids can be catalyzed and split into correspondingly glycine and aldehydes, and then acquisition pair
Reflect pure D- beta-hydroxies a-amino acid (as shown in Figure 1).
The invention further relates to preparing L- beta-hydroxies a-amino acid or D- beta-hydroxy a-amino acids in aforementioned manners, and by its
Applied to production human food, animal feed, cosmetics or pharmaceutical product use.
Classification And Nomenclature：Enterobacter cloacae tzyx2
Latin literary fame：Enterobacter cloacae tzyx2
Depositary institution：China typical culture collection center
Address：Wuhan, China Wuhan University
Preservation date：On June 24th, 2012
Deposit number：CCTCC NO:M 2012240.
Brief description of the drawings
Fig. 1, enterobacter cloacae (Enterobacter cloacae tzyx2) thick Enzyme catalyzed synthesis of L-threonine aldolase
L- beta-hydroxies a-amino acid reacts.
Fig. 2, structure Escherichia coli sequencing vector pGEMT-LTA.
Fig. 3, structure coli expression carrier pET11-LTA.
Fig. 4, enterobacter cloacae (Enterobacter cloacae tzyx2) thick Enzyme catalyzed synthesis of L-threonine aldolase
L- Phenserines react.
Embodiment
The present invention is expanded on further with reference to specific embodiment.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limitation the scope of the present invention.
Embodiment 1 separates the nucleotide sequence of L-threonine aldolase from enterobacter cloacae
According to《Molecular Cloning:A Laboratory guide》The method of offer is extracted total from the enterobacter cloacae thalline of culture 36 hours
DNA, take 7 μ g for template carry out PCR.According to the L-threonine aldolase sequences Design delivered, the above
The DNA for stating extraction expands for template in the enterprising performing PCR of T-Gradient PCR instruments (Biometra companies), reaction the primer,
Component and amplification condition are as follows：
Primer 1:5’-ATGATTGATTTACGCAGTGATACCG-3’
Primer 2：5’-TTAACGCTGTAAAAACGCCTGCCAG-3’
Amplification condition：94 DEG C of denaturation 3min, then 30 circulations are carried out with 94 DEG C of 1min, 58 DEG C of 1min, 72 DEG C of 1min,
Last 72 DEG C of 10min.Agarose gel electrophoresis testing result shows that amplification obtains size about 1000bp fragment, uses UNIQ-
10 pillar PCR primer purification kits (Shanghai Sangon Biological Engineering Technology And Service Co., Ltd's product) reclaim, recovery fragment
It is subcloned into sequencing vector pGEM-T (Promega Products)；Plasmid construction result is shown in accompanying drawing 2, and constructed contains L-
The plasmid of threonine aldolase gene is named as pGEMT-LTA.Connection product, which is transformed into, uses CaCl2The Escherichia coli of method processing
DH5 α, the overnight incubation on the LB solid mediums containing ampicillin (final concentration of 100 μ g/ml)；Grown on picking flat board
White colony, access containing ampicillin (final concentration of 60 μ g/ml) LB fluid nutrient mediums in overnight incubation, be collected by centrifugation
Thalline by alkaline lysis [Sambroook, et al., 1989, Molecular Cloning, a Laboratory Manual,
Cold Spring Harbor Laboratory, New York, p19-21] extraction plasmid, through NcoI and SacI double digestions and
PCR amplification identifications are correct, are sequenced (Shanghai Sangon Biological Engineering Technology And Service Co., Ltd).Sequencing result display expands to obtain
Clip size be 1002bp, by its coding amino acid sequence Blast programs (Basic is used in Genbank databases
Local Alignment seatch tool) [Altschul SF et al, 1997, Nucleic Acids Res.25:
3389-3402] Homology search is carried out, retrieval result shows that with the most like homologous fragment of the fragment be L-threonine aldolase,
It is but not fully identical, it was demonstrated that institute's amplified fragments are new L-threonine aldolase.
Embodiment 2：The structure of Recombinant protein expression carrier
According to SEQ ID NO:Coding region sequence shown in 1, designing one pair of genes specificity amplification primer, to separate its potential
Open reading frame sequence：
Primer 3：5’-GGCTCTAGA ATGATTGATTTACGCAGTGATACCG-3’；
Primer 4：5’-GCCGGATCC TTAACGCTGTAAAAACGCCTGCCAG-3’；
The 5` ends black matrix of this two primers contains Xba I and the restriction enzyme sites of BamH I respectively.Amplification condition used and reaction
Component is same as above, and the sequencing result of amplified production is shown and SEQ ID NO:Sequence shown in 1 is consistent.Then 50 μ l PCR primers are taken
Double digestion is carried out respectively with 1 μ l pET11a (Invitrogen companies), reclaims digestion large fragment, and with T4 ligases in 4 degree of ice
Connected overnight in case.Connection product converts bacillus coli DH 5 alpha, by plasmid extraction and PCR screening positive clones, and is surveyed
Sequence is identified.Plasmid construction result is shown in accompanying drawing 3, the constructed colibacillus expression plasmid containing L-threonine aldolase gene
It is named as pET-LTA.Connection product converts bacillus coli DH 5 alpha, and Screening and Identification goes out recombinant plasmid.
Embodiment 3：The preparation of the thick enzyme of L-threonine aldolase
The positive transformant single bacterium colony of identification is inoculated in the SOC culture mediums containing Amp (100 μ g/ml), 37 DEG C of shaken cultivations
Overnight, bacteria concentration reaches OD600When=1, it is inoculated in 1% in the LB culture mediums containing Amp (100 μ g/ml), 37 DEG C are continued culture extremely
OD600In 1.2~1.5 when, cell is harvested by centrifugation.Cell is resuspended with 0.1M phosphate buffer solutions, 20000 after ultrasonication
Leave the heart 1 hour, harvest supernatant is the thick enzyme of L-threonine aldolase, is dispensed, -20 DEG C of preservations.
Embodiment 4：The thick Enzyme catalyzed synthesis L- Phenserines (accompanying drawing 4) of L-threonine aldolase
Enzyme-activity unit defines:At room temperature, catalysis L-threonine per minute decomposes generation 1mmol acetaldehyde, is defined as 1 enzyme activity
Unit.
Catalytic condition：Buffer solution, KH2PO4, 50mM；PH8.0.1ml reaction systems：L-TA, 77U；PLP, 13ng；Benzene first
Aldehyde, 10mg；Glycine, 75mg.Reaction temperature, 25 DEG C, the reaction time, 45min.
HPLC testing conditions：Instrument, Agilent1260；Mobile phase, 0.1% sodium heptanesulfonate：Methanol=80：20；Chromatogram
Post, C18,5 μm, 4.6 × 150mm；Column temperature, 20 DEG C；Detector, it is ultraviolet；Wavelength, 225nm；Solvent, methanol.
Testing result：Yield 80%, ee>99%.
Claims (1)
1. application of a kind of enterobacter cloacae in L- Phenserines are prepared, it is characterised in that the enterobacter cloacae is preservation
Numbering is CCTCC NO：M 2012240 enterobacter cloacae.
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| US11512303B2 (en) | 2017-05-27 | 2022-11-29 | Enzymaster (Ningbo) Bio-Engineering Co., Ltd. | Engineered polypeptides and their applications in the synthesis of beta-hydroxy-alpha-amino acids |
| CN110272856B (en) * | 2019-05-08 | 2022-05-03 | 江南大学 | Recombinant bacterium for expressing D-threonine aldolase and construction method and application thereof |
| CN113322248B (en) * | 2021-05-12 | 2022-10-28 | 浙江工业大学 | High-temperature-resistant L-threonine aldolase and application thereof in synthesis of p-methylsulfonylphenylserine |
| CN116376989B (en) * | 2022-04-11 | 2023-10-24 | 元素驱动(杭州)生物科技有限公司 | Method for preparing keto acid and application of method in preparation of amino acid or amino acid derivative |
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Non-Patent Citations (2)
| Title |
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| CP001918.1;Ren,Y.,et al;《GENBANK》;20140131;全文 * |
| Gene cloning, biochemical characterization and physiological role of a thermostable low-specificity L-threonine aldolase from Escherichia coli;Ji-Quan LIU，et al;《Eur. J. Biochem.》;19981231;第255卷;220-226 * |
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