CN104543802A - Method for extracting soybean oligosaccharides from soybeans - Google Patents
Method for extracting soybean oligosaccharides from soybeans Download PDFInfo
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- CN104543802A CN104543802A CN201410779690.5A CN201410779690A CN104543802A CN 104543802 A CN104543802 A CN 104543802A CN 201410779690 A CN201410779690 A CN 201410779690A CN 104543802 A CN104543802 A CN 104543802A
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Abstract
The invention discloses a method for extracting soybean oligosaccharides from soybeans. The method comprises the following steps: (1) pretreating; (2) deproteinizing; (3) performing ultrafiltration; (4) discoloring; (5) desalting; (6) concentrating; and (7) performing spray drying. The process equipment needed by the method is simple and is low in cost, the efficiency is high, the waste liquid discharge amount is small, the environmental pollution is slight, the problem that the traditional method for producing the soybean oligosaccharides is high in cost and high in energy consumption is solved, and the method disclosed by the invention is suitable for large-scale production.
Description
Technical field
The invention belongs to food processing field, specifically a kind of extracting method of soyabean oligosaccharides.
Background technology
Soyabean oligosaccharides is total address of contained soluble-carbohydrate in soybean, and it is functional oligose, belongs to the one of prebiotics.Because compound sugar has the very high potential of application in food, medicine and feed addictive etc., extraction and the function of research soyabean oligosaccharides have very large meaning.The subject matter of current existence is that the key technology of separation and purification soyabean oligosaccharides fails to solve, and realizes industrialization and also there is many problems.In the separation and purification process of soyabean oligosaccharides, the separation of protein is the most key factor, and its separating effect directly has influence on the technique of postorder, the quality of product and yield.Decolouring, desalination are also the important steps of soyabean oligosaccharides separation and purification, and it directly affects quality and the production cost thereof of product.A lot of Methods For Purification degree is not high at present, and method is complicated.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of purification efficiency high, the simple to operate method extracting soyabean oligosaccharides from soybean.The present invention is by the following technical solutions:
From soybean, extract a method for soyabean oligosaccharides, comprise the following steps:
(1) pretreatment: the distilled water adding 20-30 times of weight in defatted soy flour, after ultrasonic wave process 5-10min, stirs 3-5h under being heated to 90-100 degree, collected by suction supernatant.
(2) removing protein: supernatant step (1) obtained is by adding the calcium chloride solution that concentration is 3%-5% after column chromatography removing sucrose, 20-30min is heated under 80-90 degree, then with hydrochloric acid, pH value being adjusted to 4.3-4.5 makes protein coagulation precipitate, and obtains supernatant after high speed centrifugation.
(3) ultrafiltration: the supernatant that step (2) obtains is carried out ultrafiltration removing residual protein.
(4) decolour: the permeate obtained after ultrafiltration is decoloured with under the macroporous anion exchange resin normal temperature of 1/10 volume.
(5) desalination: the eluent strongly acidic cation-exchange obtained through decolouring and strong basic type anion-exchange resin carry out desalting processing under 50-60 degree, and flow rate of liquid is 30-35m3/m3.h.
(6) concentrated: step (5) to be carried out Vacuum Concentration through the liquid of desalting processing under 70 degree and obtains soy oligosaccharides syrup.
(7) spraying dry: the concentrated syrup obtained is after press atomization, and drying obtains powdered soy bean compound sugar finished product in drying tower.
Ultrasonic wave processing power described in step (1) is 50-100w.
In step (3), ultrafiltrate temperature is 40-50 degree, and ultrafiltration pressure is 3-5psi, and retaining molecular weight is 10000.
Beneficial effect of the present invention: the process equipment needed for this method is simple, cost is low, and efficiency is high, and discharging of waste liquid is few, and environmental pollution is low, solve produced in conventional processes soyabean oligosaccharides cost high, the large problem that consumes energy is applicable to suitability for industrialized production.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment 1
From soybean, extract a method for soyabean oligosaccharides, comprise the following steps:
(1) pretreatment: the distilled water adding 20 times of weight in defatted soy flour is, after processing 5min under the ultrasonic wave of 50w, stir 3h, collected by suction supernatant under being heated to 90 degree at power.
(2) removing protein: supernatant step (1) obtained is by adding the calcium chloride solution that concentration is 3% after column chromatography removing sucrose, 20min is heated under 80 degree, then with hydrochloric acid, pH value being adjusted to 4.3 makes protein coagulation precipitate, and obtains supernatant after high speed centrifugation.
(3) ultrafiltration: the supernatant that step (2) obtains is carried out ultrafiltration removing residual protein, ultrafiltrate temperature is 40 degree, and ultrafiltration pressure is 3, and retaining molecular weight is 10000..
(4) decolour: the permeate obtained after ultrafiltration is decoloured with under the macroporous anion exchange resin normal temperature of 1/10 volume.
(5) desalination: the eluent strongly acidic cation-exchange obtained through decolouring and strong basic type anion-exchange resin carry out desalting processing under 50 degree, and flow rate of liquid is 30m3/m3.h.
(6) concentrated: by step 5) under 70 degree, carry out Vacuum Concentration through the liquid of desalting processing and obtain soy oligosaccharides syrup.
(7) spraying dry: the concentrated syrup obtained is after press atomization, and drying obtains powdered soy bean compound sugar finished product in drying tower.
Embodiment 2
From soybean, extract a method for soyabean oligosaccharides, comprise the following steps:
(1) pretreatment: the distilled water adding 30 times of weight in defatted soy flour is, after processing 10min under the ultrasonic wave of 100w, stir 5h, collected by suction supernatant under being heated to 100 degree at power.
(2) removing protein: supernatant step (1) obtained is by adding the calcium chloride solution that concentration is 5% after column chromatography removing sucrose, 30min is heated under 90 degree, then with hydrochloric acid, pH value being adjusted to 4.5 makes protein coagulation precipitate, and obtains supernatant after high speed centrifugation.
(3) ultrafiltration: the supernatant that step (2) obtains is carried out ultrafiltration removing residual protein, ultrafiltrate temperature is 50 degree, and ultrafiltration pressure is 5psi, and retaining molecular weight is 10000..
(4) decolour: the permeate obtained after ultrafiltration is decoloured with under the macroporous anion exchange resin normal temperature of 1/10 volume.
(5) desalination: the eluent strongly acidic cation-exchange obtained through decolouring and strong basic type anion-exchange resin carry out desalting processing under 60 degree, and flow rate of liquid is 35m3/m3.h.
(6) concentrated: step (5) to be carried out Vacuum Concentration through the liquid of desalting processing under 70 degree and obtains soy oligosaccharides syrup.
(7) spraying dry: the concentrated syrup obtained is after press atomization, and drying obtains powdered soy bean compound sugar finished product in drying tower.
Embodiment 3
From soybean, extract a method for soyabean oligosaccharides, comprise the following steps:
(1) pretreatment: the distilled water adding 25 times of weight in defatted soy flour is, after processing 8min under the ultrasonic wave of 80w, stir 4h, collected by suction supernatant under being heated to 95 degree at power.
(2) removing protein: supernatant step (1) obtained is by adding the calcium chloride solution that concentration is 4% after column chromatography removing sucrose, 25min is heated under 85 degree, then with hydrochloric acid, pH value being adjusted to 4.4 makes protein coagulation precipitate, and obtains supernatant after high speed centrifugation.
(3) ultrafiltration: the supernatant that step (2) obtains is carried out ultrafiltration removing residual protein, ultrafiltrate temperature is 45 degree, and ultrafiltration pressure is 4psi, and retaining molecular weight is 10000.
(4) decolour: the permeate obtained after ultrafiltration is decoloured with under the macroporous anion exchange resin normal temperature of 1/10 volume.
(5) desalination: the eluent strongly acidic cation-exchange obtained through decolouring and strong basic type anion-exchange resin carry out desalting processing under 55 degree, and flow rate of liquid is 33m3/m3.h.
(6) concentrated: by step 5) under 70 degree, carry out Vacuum Concentration through the liquid of desalting processing and obtain soy oligosaccharides syrup.
(7) spraying dry: the concentrated syrup obtained is after press atomization, and drying obtains powdered soy bean compound sugar finished product in drying tower.
Above embodiment is only in order to illustrate that technical scheme of the present invention is not intended to limit, and to not departing from any amendment of spirit and scope of the invention or equivalent replacement, it all should be encompassed in the middle of right of the present invention.
Claims (3)
1. from soybean, extract a method for soyabean oligosaccharides, comprise the following steps:
(1) pretreatment: the distilled water adding 20-30 times of weight in defatted soy flour, after ultrasonic wave process 5-10min, stirs 3-5h under being heated to 90-100 degree, collected by suction supernatant;
(2) removing protein: supernatant step (1) obtained is by adding the calcium chloride solution that concentration is 3%-5% after column chromatography removing sucrose, 20-30min is heated under 80-90 degree, then with hydrochloric acid, pH value being adjusted to 4.3-4.5 makes protein coagulation precipitate, and obtains supernatant after high speed centrifugation;
(3) ultrafiltration: the supernatant that step (2) obtains is carried out ultrafiltration removing residual protein;
(4) decolour: the permeate obtained after ultrafiltration is decoloured with under the macroporous anion exchange resin normal temperature of 1/10 volume;
(5) desalination: the eluent strongly acidic cation-exchange obtained through decolouring and strong basic type anion-exchange resin carry out desalting processing under 50-60 degree, and flow rate of liquid is 30-35m
3/ m
3.h;
(6) concentrated: step (5) to be carried out Vacuum Concentration through the liquid of desalting processing under 70 degree and obtains soy oligosaccharides syrup;
(7) spraying dry: the concentrated syrup obtained is after press atomization, and drying obtains powdered soy bean compound sugar finished product in drying tower.
2. a kind of method extracting soyabean oligosaccharides from soybean according to claim 1, is characterized in that: the ultrasonic wave processing power described in step (1) is 50-100w.
3. a kind of method extracting soyabean oligosaccharides from soybean according to claim 1, is characterized in that: in step (3), ultrafiltrate temperature is 40-50 degree, and ultrafiltration pressure is 3-5psi, and retaining molecular weight is 10000.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410779690.5A CN104543802A (en) | 2014-12-17 | 2014-12-17 | Method for extracting soybean oligosaccharides from soybeans |
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| CN201410779690.5A CN104543802A (en) | 2014-12-17 | 2014-12-17 | Method for extracting soybean oligosaccharides from soybeans |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108640953A (en) * | 2018-04-27 | 2018-10-12 | 中国农业大学 | Using macroreticular resin to the process for purification of pumpkin oligosaccharide |
| CN109734753A (en) * | 2018-11-27 | 2019-05-10 | 安徽博悦生物科技有限公司 | A kind of extracting method of soyabean oligosaccharides |
| CN111139318A (en) * | 2019-12-11 | 2020-05-12 | 黑龙江省农垦龙王食品有限公司 | Method for extracting soybean oligosaccharide |
| CN114163486A (en) * | 2021-12-22 | 2022-03-11 | 云南三七科技有限公司 | Method for preparing oligosaccharide from pseudo-ginseng |
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| CN1284504A (en) * | 2000-08-24 | 2001-02-21 | 李振铎 | Methof of extracting isoflavone, saponin, oligosaccharide and protein simultaneously from defatted soybean dregs |
| CN102827687A (en) * | 2012-09-17 | 2012-12-19 | 东北农业大学 | Extraction method of soybean oligosaccharides |
| CN103589766A (en) * | 2013-11-22 | 2014-02-19 | 东北农业大学 | Method for simultaneously extracting soybean peptide and soybean oligosaccharide from aqueous phase produced through aqueous enzymatic method |
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2014
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1284504A (en) * | 2000-08-24 | 2001-02-21 | 李振铎 | Methof of extracting isoflavone, saponin, oligosaccharide and protein simultaneously from defatted soybean dregs |
| CN102827687A (en) * | 2012-09-17 | 2012-12-19 | 东北农业大学 | Extraction method of soybean oligosaccharides |
| CN103589766A (en) * | 2013-11-22 | 2014-02-19 | 东北农业大学 | Method for simultaneously extracting soybean peptide and soybean oligosaccharide from aqueous phase produced through aqueous enzymatic method |
Non-Patent Citations (1)
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108640953A (en) * | 2018-04-27 | 2018-10-12 | 中国农业大学 | Using macroreticular resin to the process for purification of pumpkin oligosaccharide |
| CN108640953B (en) * | 2018-04-27 | 2020-12-15 | 中国农业大学 | Refining method of pumpkin oligosaccharide by adopting macroporous resin |
| CN109734753A (en) * | 2018-11-27 | 2019-05-10 | 安徽博悦生物科技有限公司 | A kind of extracting method of soyabean oligosaccharides |
| CN111139318A (en) * | 2019-12-11 | 2020-05-12 | 黑龙江省农垦龙王食品有限公司 | Method for extracting soybean oligosaccharide |
| CN114163486A (en) * | 2021-12-22 | 2022-03-11 | 云南三七科技有限公司 | Method for preparing oligosaccharide from pseudo-ginseng |
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Application publication date: 20150429 |