CN104351145B - A kind of device collecting virgin fruit bat - Google Patents

A kind of device collecting virgin fruit bat Download PDF

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Publication number
CN104351145B
CN104351145B CN201410623829.7A CN201410623829A CN104351145B CN 104351145 B CN104351145 B CN 104351145B CN 201410623829 A CN201410623829 A CN 201410623829A CN 104351145 B CN104351145 B CN 104351145B
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China
Prior art keywords
fruit bat
virgin
pipe
collector
insect
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Expired - Fee Related
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CN201410623829.7A
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Chinese (zh)
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CN104351145A (en
Inventor
张徐波
董玮
李涛
张建珍
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Shanxi University
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Shanxi University
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Priority to CN201410623829.7A priority Critical patent/CN104351145B/en
Publication of CN104351145A publication Critical patent/CN104351145A/en
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Publication of CN104351145B publication Critical patent/CN104351145B/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

Abstract

A kind of device collecting virgin fruit bat, relates to Genetics experiment device.Solving existing virgin's fruit bat collector structure complicated, cost is high, the problem collecting cumbersome.The present invention includes: insect raises pipe, it is placed on the feed raised bottom pipe, is entrenched in the ventilative pipe close raising pipe port, it is characterised in that: described insect is raised and arranges virgin's fruit bat collector in the middle part of pipe, virgin's fruit bat collector is set in together by multiple pipes, and is connected by cross is fixing;Spacing between adjacent pipe is slightly less than the height of an adult fruit bat;The centre bore of cross inserts piece central shaft of pipe being positioned at collector center, and enters in feed.The present invention is used for collecting fruit bat virgin fly, has rational in infrastructure, simple to operate, collects the advantage that virgin's fruit bat degree of accuracy is high.

Description

A kind of device collecting virgin fruit bat
Technical field
The present invention relates to the genetics experiments of fruit bat, particularly to a kind of device collecting fruit bat virgin fly.
Background technology
Fruit bat is classical model organism, has the history of life short, and filial generation is many, easily raises, and chromosome number is few, The advantages such as genetic tool is comprehensive, are widely used in the scientific research of science of heredity and Developmental Biology.The most great Scientific discovery be all made by what fruit bat was accomplished, fruit bat is a kind of good experiment material thus.
The genetics experiments of fruit bat, needs to hybridize different mutant strains or transgenic strain.Hybridize A crucial step is to select the virgin fly of non-mating, and then the male worm with other strains hybridizes.Select virgin Fly is item very hard work, the serious efficiency restricting genetics experiments.
At present, the method collecting fruit bat virgin fly, mainly examine under a microscope the biological property of fruit bat, Carry out hand picking.Virgin fly to be mainly characterized by health body colour shallow, wing is soft and tender, and belly turns white and has The unfixed dark color spots in position, has the female fly of this feature substantially to can be determined that as virgin fly.Hand picking Virgin fly is wasted time and energy, and selects result and affected by experience and subjective judgement, varies with each individual, it is difficult to ensure The accuracy selected.
Fruit bat is after emergence, and female fly is sought a spouse by male fly.Male fly shows fixing sequencing behavior pattern, Including: towards female fly, pat female fly, follow female fly, vibration side wing female fly is sung " song of seeking a spouse ", Lick female fly afterbody, attempt mating and mating.During mating, male fly is stacked in female fly, accordingly, it would be desirable to higher Spatial altitude.Within after emergence about 2~6 hours, mating (25 DEG C) can be completed.
Once virgin fruit bat post-coitum, can leave in internal the sperm of Male Drosophila, self-fertilization by several times. If virgin fly could not accurately be selected, the correctness of progeny genotypes can be directly affected, ultimately result in experiment Failure.
2011, Taiwan Tsing-Hua University successfully researched and developed whole world stem virgin's fruit bat collector.The fruit that will sprout wings Fly pupa is put in test tube, when fruit bat sprouts wings, lures it to queue up through narrow from the other end fragrance of test tube Passage, lower section phtographic lens by special lithographic pictures such as the belly spot of fruit bat, sexual organs, passes to computer quick again Interpretation also starts Filtering system, is then automatically collected by virgin fly.This virgin's fruit bat collector, computer and Other attached devices are many, and structure is complicated, and cost is high.During operation, need target drosophila strains is placed in dress In putting, waiting the longer time, collection process lasts till that abundant fruit bat sprouts wings, and not only needs to differentiate female Hero, virgin fly to be distinguished and non-virgin fly.
Summary of the invention
The purpose of the present invention is intended to overcome the shortcoming of prior art, it is provided that a kind of device collecting virgin fruit bat, Solving existing virgin's fruit bat collector structure complicated, cost is high, and the operating time is long, collects the skill of cumbersome Art problem.
The present invention is achieved by the following technical solutions:
A kind of device collecting virgin fruit bat, including: insect raises pipe, is placed on insect and raises bottom pipe Feed, is entrenched in insect and raises the ventilative pipe close of pipe port, it is characterised in that: described insect is raised in the middle part of pipe Placing virgin's fruit bat collector, described virgin's fruit bat collector is set in together by multiple pipes, and by cross Frame is fixing to be connected;Spacing between adjacent described pipe, is slightly less than the height of an adult fruit bat;Described ten The centre bore of cabinet frame inserts piece central shaft of described pipe being positioned at collector center, described central shaft and institute Stating cross to fix, lower end is stretched in described feed.
Further, the upper end of described central shaft is provided with flexible little draw ring.
The present invention compared with prior art, is sleeved on by multiple pipes owing to raising to be provided with in the middle part of pipe insect Spacing between virgin fruit bat collector, and adjacent pipe is slightly less than the height of an adult fruit bat together, because of This, enter the fruit bat between adjacent pipe, and narrow space limits the behavior that they are sought a spouse, and prevents plumage The fruit bat changed carries out mating.After taking out fruit bat from collector, it is only necessary to give up Male Drosophila, remaining female Property fruit bat is virgin fly, so drastically increases operating efficiency.Multiple tube wall adds larvae pupation Space, thus add the quantity of adult eclosion.
The present invention is used for collecting fruit bat virgin fly, has rational in infrastructure, simple to operate, collects virgin fruit bat accurate The advantage that exactness is high.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention;
The A-A profile of Fig. 1 that Fig. 2 is.
Detailed description of the invention
Embody the exemplary embodiments of feature of present invention and advantage, will describe the most in detail.Ying Li Solve is that the present invention can have various changes in different embodiments, and it is all without departing from the guarantor of the present invention Protect scope, and explanation therein is inherently illustrated as being used, and be not used to limit the present invention.
As shown in Figure 1, 2, a kind of device collecting virgin fruit bat, including: insect raises pipe 1, is placed on Insect raises the feed 2 bottom pipe 1, is entrenched in insect and raises the ventilative pipe close 3 of pipe 1 port, described insect Raising and place virgin's fruit bat collector 4 in the middle part of pipe 1, described virgin's fruit bat collector 4 is overlapped by multiple pipes 41 It is fitted together, and is connected by cross 42 is fixing;Spacing H between adjacent described pipe 41, is slightly less than one The height of individual adult fruit bat;The centre bore of described cross 42 inserts the described pipe 41 being positioned at collector center An interior central shaft 43, described central shaft 43 is fixing with described cross 42, and described feed 2 is stretched in lower end In.
The upper end of described central shaft 43 is provided with flexible little draw ring 44, it is simple to take out virgin's fruit bat collector 4.
The job step of the present invention:
Fruit bat just can hatch larva after laying eggs in feed 2 about 24 hours.Treat that insect raising pipe 1 has Abundant larva, the adult raised by insect in pipe 1 all transfers.Virgin's fruit bat collector 4 is placed Raising in pipe 1 to insect, central shaft 43 inserts in feed 2, the multiple pipe of virgin's fruit bat collector 4 Lower edge submerges in feed 2.Breathe freely beyond the Great Wall pipe close 3, the lower edge of ventilative pipe close 3 and virgin's fruit bat collector 4 Upper limb is in close contact, and does not stay gap.When fruit bat is to three latter stages in age, enter the space H between adjacent pipe 41 After pupating, sprouting wings, adult is limited in narrow space.During due to fruit bat mating, male worm needs to seek connections with Completing fertilization with female worm, the height limitation in this space fruit bat completes mating, therefore ensure that sprout wings female Worm is the virgin fly of non-mating, drastically increases the efficiency that virgin fly is collected.
Adult carbon dioxide anesthesia is taken out, gives up male fly, just can get virgin fly.
Larval density in pipe 41 can not be too big, and feed is in good condition, does not affect the normal size of larva. Fruit bat after sprouting wings in virgin's fruit bat collector 4 should be taken out in 72 hours.
The present invention can be embodied as the spirit without deviating from invention or essence in a variety of forms, so should manage Solving, above-described embodiment is not limited to aforesaid details, and should solve widely in claim limited range Release, therefore fall into the change in claim or its equivalent scope and remodeling, all should be claim and contained.

Claims (2)

1. collect a device for virgin fruit bat, including: insect raises pipe (1), is placed on insect and raises The feed (2) of pipe (1) bottom, is entrenched in insect and raises the ventilative pipe close (3) of pipe (1) port, its It is characterised by: described insect is raised pipe (1) middle part and places virgin's fruit bat collector (4), described virgin fruit Fly collector (4) is set in together by multiple pipes (41), and is connected by cross (42) is fixing; Spacing H between adjacent described pipe (41), is slightly less than the height of an adult fruit bat;Described cross The centre bore of frame (42) inserts in the described pipe (41) being positioned at virgin's fruit bat collector center Mandrel (43), described central shaft (43) is fixing with described cross (42), and described feed is stretched in lower end (2) in.
The device collecting virgin fruit bat the most according to claim 1, it is characterised in that: described center The upper end of axle (43) is provided with flexible little draw ring (44).
CN201410623829.7A 2014-11-07 2014-11-07 A kind of device collecting virgin fruit bat Expired - Fee Related CN104351145B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410623829.7A CN104351145B (en) 2014-11-07 2014-11-07 A kind of device collecting virgin fruit bat

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Application Number Priority Date Filing Date Title
CN201410623829.7A CN104351145B (en) 2014-11-07 2014-11-07 A kind of device collecting virgin fruit bat

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CN104351145A CN104351145A (en) 2015-02-18
CN104351145B true CN104351145B (en) 2016-08-24

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Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105404920B (en) * 2015-10-30 2017-09-19 信阳农林学院 A kind of statistical method of drosophila reproductive capacity and special statistics utensil
CN110301404B (en) * 2019-07-29 2021-07-30 湖南文理学院 Device for collecting virgo fruit flies
CN112753676A (en) * 2019-11-04 2021-05-07 何凤侠 By CO2Adult device for anaesthetizing and selecting mosquitoes and flies

Citations (4)

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Publication number Priority date Publication date Assignee Title
US4106438A (en) * 1976-09-03 1978-08-15 Nelson Roger K Method of handling Drosophila
US4212267A (en) * 1978-12-08 1980-07-15 Patterson Irvin G Insect study station
US4224898A (en) * 1979-05-24 1980-09-30 Carolina Biological Supply Company Method and means for anesthetizing insects
CN203027935U (en) * 2013-01-25 2013-07-03 浙江师范大学 Novel detachable flat bottom test tube

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US8408164B2 (en) * 2009-09-04 2013-04-02 Richard David Robinson, Jr. System and method for release and dispersion of flies or other biological control

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4106438A (en) * 1976-09-03 1978-08-15 Nelson Roger K Method of handling Drosophila
US4212267A (en) * 1978-12-08 1980-07-15 Patterson Irvin G Insect study station
US4224898A (en) * 1979-05-24 1980-09-30 Carolina Biological Supply Company Method and means for anesthetizing insects
CN203027935U (en) * 2013-01-25 2013-07-03 浙江师范大学 Novel detachable flat bottom test tube

Non-Patent Citations (3)

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"果蝇实验中海绵塞及移蝇漏斗的制作";周天宇 等;《生物学通报》;20110620;第46卷(第6期);第49-50页 *
"果蝇实验中的2个小制作";汤明 等;《生物学通报》;20101120;第45卷(第11期);第49-50页 *
"野生果蝇的收集和培养";刘金香;《生物学通报》;20001130;第35卷(第11期);第35-36页 *

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