CN104304235A - A reagent erythrocyte preserving fluid and a preparing method thereof - Google Patents
A reagent erythrocyte preserving fluid and a preparing method thereof Download PDFInfo
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- CN104304235A CN104304235A CN201410501658.0A CN201410501658A CN104304235A CN 104304235 A CN104304235 A CN 104304235A CN 201410501658 A CN201410501658 A CN 201410501658A CN 104304235 A CN104304235 A CN 104304235A
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- sodium
- chloramphenicol
- adenine
- mannitol
- glucose
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Abstract
The invention relates to a reagent erythrocyte preserving fluid and a preparing method thereof, and belongs to the field of medical examination. The preserving fluid comprises distilled water and sodium chloride. The preserving fluid and the method are characterized in that: the preserving fluid also comprises sodium citrate, ammonium chloride, disodium hydrogen phosphate, glucose, mannitol, adenine, chloramphenicol, sodium azide and amino acids, and the weight ratio of the distilled water, the sodium citrate, the ammonium chloride, the disodium hydrogen phosphate, the glucose, the mannitol, the adenine, the chloramphenicol, the sodium azide, the amino acids and the sodium chloride is 100 : 2-3 : 0.5-1 : 0.1-0.2 : 4-5 : 1-2 : 0.1-0.2 : 0.15-0.2 : 0.001-0.0025 : 1-2 : 2-3. By using the preserving agent for reagent erythrocyte preservation, the storage lifetime of the reagent erythrocytes is largely prolonged and can reach 90 days, thus largely facilitating fully-automatic micro-plate scan detection of reverse ABO typing for a large number of blood donors in a blood station, and carrying out of hemolytic disease of the newborn and difficult blood matching which are caused by incompatibility of blood group between the mother and fetus, and other works, and laying good foundations for serology examination and research on erythrocyte blood types.
Description
Technical field
The present invention relates to a kind of reagent red blood cell conserving liquid and preparation method, belong to field of medical examination.
Background technology
Preserve fresh reagent red blood cell with 5% physiological saline in existing ABO reverse type automatically scanning and other blood group serology work, storage life is 3 days.Be not easy to blood station blood donor's abo blood group in enormous quantities reverse type, and the carrying out of the work such as neonatal hemolytic disease and difficult match that causes of mother and baby's blood group incompatibility.
Summary of the invention
The object of the invention is to find out a kind of new reagent red blood cell conserving liquid and preparation method, be kept at by reagent red blood cell in reagent red blood cell conserving liquid, prolongation reagent red blood cell storage life that can be larger, reaches 90 days.
The present invention is achieved like this: a kind of reagent red blood cell conserving liquid, be made up of distilled water and sodium chloride, it is characterized in that also having sodium citrate, sal-ammoniac, sodium hydrogen phosphate, glucose, mannitol, adenine, chloramphenicol, Sodium azide and amino acid, each composition is by weight ratio:
Distilled water: sodium citrate: sal-ammoniac: sodium hydrogen phosphate: glucose: mannitol: adenine: chloramphenicol: Sodium azide: amino acid: sodium chloride=100: 2-3: 0.5-1: 0.1-0.2: 4-5: 1-2: 0.1-0.2: 0.15-0.2: 0.001-0.0025: 1-2: 2-3.
A kind of preparation method of reagent red blood cell conserving liquid, it is characterized in that, preparation process is as follows: by weight, sodium citrate, sal-ammoniac, sodium hydrogen phosphate, glucose, mannitol, adenine, chloramphenicol, Sodium azide, amino acid and sodium chloride is added successively in 100 parts of distilled water, add a kind of composition to dissolving at every turn, add lower a kind of composition again, until all composition adds, after dissolving completely, obtain reagent red blood cell conserving liquid; The weight ratio adding composition is distilled water: sodium citrate: sal-ammoniac: sodium hydrogen phosphate: glucose: mannitol: adenine: chloramphenicol: Sodium azide: amino acid: sodium chloride=100: 2-3: 0.5-1: 0.1-0.2: 4-5: 1-2: 0.1-0.2: 0.15-0.2: 0.001-0.0025: 1-2: 2-3.
Preferably, also sodium citrate, sal-ammoniac, sodium hydrogen phosphate, glucose, mannitol, adenine, chloramphenicol, Sodium azide, amino acid, sodium chloride can once be added in 100 parts of distilled water, can add by any order, after various composition dissolves completely, obtain reagent red blood cell conserving liquid.
Preferably, the reagent red blood cell conserving liquid prepared will keep in Dark Place at the temperature of 2-10 DEG C.
Preserve reagent red blood cell with a kind of reagent red blood cell conserving liquid of the present invention, prolongation reagent red blood cell storage life that can be larger, reaches 90 days.Greatly facilitate the full-automatic micro plate Scanning Detction of blood station blood donor in enormous quantities abo blood group reverse type, the carrying out of the work such as neonatal hemolytic disease that mother and baby's blood group incompatibility causes and solidifying difficult match, also established good basis for erythrocyte blood type serologic test and research work.
Embodiment
Embodiment one:
A kind of reagent red blood cell conserving liquid, be made up of distilled water, sodium chloride, it is characterized in that also having sodium citrate, sal-ammoniac, sodium hydrogen phosphate, glucose, mannitol, adenine, chloramphenicol, Sodium azide, amino acid, its weight ratio is: distilled water: sodium citrate: sal-ammoniac: sodium hydrogen phosphate: glucose: mannitol: adenine: chloramphenicol: Sodium azide: amino acid: sodium chloride=100: 2-3: 0.5-1: 0.1-0.2: 4-5: 1-2: 0.1-0.2: 0.15-0.2: 0.001-0.00125: 1-2: 2-3.
A kind of preparation method of reagent red blood cell conserving liquid, it is characterized in that adding sodium citrate, sal-ammoniac, sodium hydrogen phosphate, glucose, mannitol, adenine, chloramphenicol, Sodium azide, amino acid, sodium chloride successively in 100 parts of distilled water, can add by any order, add a kind of composition to dissolving at every turn, add lower a kind of composition again, until all composition adds; The weight ratio adding composition is distilled water: sodium citrate: sal-ammoniac: sodium hydrogen phosphate: glucose: mannitol: adenine: chloramphenicol: Sodium azide: amino acid: sodium chloride=100: 2-3: 0.5-1: 0.1-0.2: 4-5: 1-2: 0.1-0.2: 0.15-0.2: 0.001-0.0025: 1-2: 2-3.The reagent red blood cell conserving liquid prepared will keep in Dark Place at the temperature of 2-10 DEG C.
Embodiment two:
A kind of reagent red blood cell conserving liquid, be made up of distilled water, sodium chloride, it is characterized in that also having sodium citrate, sal-ammoniac, sodium hydrogen phosphate, glucose, mannitol, adenine, chloramphenicol, Sodium azide, amino acid, its weight ratio is: distilled water: sodium citrate: sal-ammoniac: sodium hydrogen phosphate: glucose: mannitol: adenine: chloramphenicol: Sodium azide: amino acid: sodium chloride=100: 3-4: 1-1.5: 0.2-0.4: 5-6: 2-4: 0.2-0.4: 0.3-0.4: 0.002-0.0025: 2-4: 2-3.
A kind of preparation method of reagent red blood cell conserving liquid, it is characterized in that adding sodium citrate, sal-ammoniac, sodium hydrogen phosphate, glucose, mannitol, adenine, chloramphenicol, Sodium azide, amino acid, sodium chloride successively in 100 parts of distilled water, can add by any order, add a kind of composition to dissolving at every turn, add lower a kind of composition again, until all composition adds; The weight ratio adding composition is distilled water: sodium citrate: sal-ammoniac: sodium hydrogen phosphate: glucose: mannitol: adenine: chloramphenicol: Sodium azide: amino acid: sodium chloride=100: 3-4: 1-1.5: 0.2-0.4: 5-6: 2-4: 0.2-0.4: 0.3-0.4: 0.002-0.0025: 2-4: 2-3.The reagent red blood cell conserving liquid prepared will keep in Dark Place at the temperature of 2-10 DEG C.
Embodiment three:
A kind of reagent red blood cell conserving liquid, be made up of distilled water, sodium chloride, it is characterized in that also having sodium citrate, sal-ammoniac, sodium hydrogen phosphate, glucose, mannitol, adenine, chloramphenicol, Sodium azide, amino acid, its weight ratio is: distilled water: sodium citrate: sal-ammoniac: sodium hydrogen phosphate: glucose: mannitol: adenine: chloramphenicol: Sodium azide: amino acid: sodium chloride=100: 1-2: 0.25-0.5: 0.05-0.1: 2-3: 0.5-1: 0.05-0.1: 0.1-0.15: 0.001-0.0015: 0.1-0.2: 1-2.
A kind of preparation method of reagent red blood cell conserving liquid, it is characterized in that adding sodium citrate, sal-ammoniac, sodium hydrogen phosphate, glucose, mannitol, adenine, chloramphenicol, Sodium azide, amino acid, sodium chloride successively in 100 parts of distilled water, can add by any order, add a kind of composition to dissolving at every turn, add lower a kind of composition again, until all composition adds; The weight ratio adding composition is distilled water: sodium citrate: sal-ammoniac: sodium hydrogen phosphate: glucose: mannitol: adenine: chloramphenicol: Sodium azide: amino acid: sodium chloride=100: 1-2: 0.25-0.5: 0.05-0.1: 2-3: 0.5-1: 0.05-0.1: 0.1-0.15: 0.001-0.0015: 0.1-0.2: 1-2.The reagent red blood cell conserving liquid prepared will keep in Dark Place at the temperature of 2-10 DEG C.
Embodiments of the present invention are explained in detail above in conjunction with the embodiments; but protection scope of the present invention is not limited to above-described embodiment; in the ken that those of ordinary skill in the art possess, can also make a variety of changes under the prerequisite not departing from present inventive concept.
Claims (3)
1. a reagent red blood cell conserving liquid, is made up of distilled water and sodium chloride, and it is characterized in that also having sodium citrate, sal-ammoniac, sodium hydrogen phosphate, glucose, mannitol, adenine, chloramphenicol, Sodium azide and amino acid, each composition is by weight ratio:
Distilled water: sodium citrate: sal-ammoniac: sodium hydrogen phosphate: glucose: mannitol: adenine: chloramphenicol: Sodium azide: amino acid: sodium chloride=100: 2-3: 0.5-1: 0.1-0.2: 4-5: 1-2: 0.1-0.2: 0.15-0.2: 0.001-0.0025: 1-2: 2-3.
2. the preparation method of a reagent red blood cell conserving liquid, it is characterized in that, preparation process is as follows: by weight, sodium citrate, sal-ammoniac, sodium hydrogen phosphate, glucose, mannitol, adenine, chloramphenicol, Sodium azide, amino acid and sodium chloride is added successively in 100 parts of distilled water, add a kind of composition to dissolving at every turn, add lower a kind of composition again, until all composition adds, after dissolving completely, obtain reagent red blood cell conserving liquid; The weight ratio adding composition is distilled water: sodium citrate: sal-ammoniac: sodium hydrogen phosphate: glucose: mannitol: adenine: chloramphenicol: Sodium azide: amino acid: sodium chloride=100: 2-3: 0.5-1: 0.1-0.2: 4-5: 1-2: 0.1-0.2: 0.15-0.2: 0.001-0.0025: 1-2: 2-3.
3. the preparation method of a kind of reagent red blood cell conserving liquid as claimed in claim 2, it is characterized in that, sodium citrate, sal-ammoniac, sodium hydrogen phosphate, glucose, mannitol, adenine, chloramphenicol, Sodium azide, amino acid, sodium chloride is once added in 100 parts of distilled water, add by any order, obtain reagent red blood cell conserving liquid after various composition dissolves completely, the reagent red blood cell conserving liquid prepared will keep in Dark Place at the temperature of 2-10 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410501658.0A CN104304235A (en) | 2014-09-27 | 2014-09-27 | A reagent erythrocyte preserving fluid and a preparing method thereof |
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| CN201410501658.0A CN104304235A (en) | 2014-09-27 | 2014-09-27 | A reagent erythrocyte preserving fluid and a preparing method thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109566602A (en) * | 2019-01-04 | 2019-04-05 | 宁波艾捷康宁生物科技有限公司 | A kind of alserver's solution and save set |
| WO2020106623A1 (en) * | 2018-11-19 | 2020-05-28 | New Health Sciences, Inc. | Methods for the preservation of reagent red blood cells using carbon monoxide |
| CN111513059A (en) * | 2020-05-07 | 2020-08-11 | 天津德祥生物技术有限公司 | Erythrocyte preservation solution and application thereof |
| CN111713487A (en) * | 2020-06-30 | 2020-09-29 | 上海市血液中心 | Reagent erythrocyte preservation system and preparation method thereof |
| CN112684192A (en) * | 2020-12-25 | 2021-04-20 | 天津市宝坻区人民医院 | New application of ammonium bradykinin in ABO blood group reverse typing kit |
| CN112684191A (en) * | 2020-12-04 | 2021-04-20 | 上海润普生物技术有限公司 | ABO blood type positive and negative shaping and Rh blood type detection card and preparation method thereof |
| CN113812395A (en) * | 2021-09-24 | 2021-12-21 | 合肥天一生物技术研究所有限责任公司 | Erythrocyte preservation solution |
| CN114732008A (en) * | 2022-06-13 | 2022-07-12 | 深圳瑞亚力集团有限公司 | Erythrocyte preservation solution, preparation method thereof and erythrocyte suspension |
| CN115777695A (en) * | 2023-02-07 | 2023-03-14 | 成都海默云因医学检验实验室有限公司 | Compound preservation solution suitable for morphological examination of cerebrospinal fluid cells and preparation method thereof |
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| CN1206537C (en) * | 2002-11-14 | 2005-06-15 | 北京市红十字血液中心 | Reagent red blood cell |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020106623A1 (en) * | 2018-11-19 | 2020-05-28 | New Health Sciences, Inc. | Methods for the preservation of reagent red blood cells using carbon monoxide |
| CN109566602A (en) * | 2019-01-04 | 2019-04-05 | 宁波艾捷康宁生物科技有限公司 | A kind of alserver's solution and save set |
| CN111513059A (en) * | 2020-05-07 | 2020-08-11 | 天津德祥生物技术有限公司 | Erythrocyte preservation solution and application thereof |
| CN111713487A (en) * | 2020-06-30 | 2020-09-29 | 上海市血液中心 | Reagent erythrocyte preservation system and preparation method thereof |
| CN112684191A (en) * | 2020-12-04 | 2021-04-20 | 上海润普生物技术有限公司 | ABO blood type positive and negative shaping and Rh blood type detection card and preparation method thereof |
| CN112684192A (en) * | 2020-12-25 | 2021-04-20 | 天津市宝坻区人民医院 | New application of ammonium bradykinin in ABO blood group reverse typing kit |
| CN113812395A (en) * | 2021-09-24 | 2021-12-21 | 合肥天一生物技术研究所有限责任公司 | Erythrocyte preservation solution |
| CN114732008A (en) * | 2022-06-13 | 2022-07-12 | 深圳瑞亚力集团有限公司 | Erythrocyte preservation solution, preparation method thereof and erythrocyte suspension |
| CN115777695A (en) * | 2023-02-07 | 2023-03-14 | 成都海默云因医学检验实验室有限公司 | Compound preservation solution suitable for morphological examination of cerebrospinal fluid cells and preparation method thereof |
| CN115777695B (en) * | 2023-02-07 | 2023-05-12 | 成都海默云因医学检验实验室有限公司 | Composite preservation solution suitable for cerebrospinal fluid cell morphology examination and preparation method thereof |
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Application publication date: 20150128 |