CN104286030A - Biological enzyme biological slime inhibitor for circulating cooling water - Google Patents
Biological enzyme biological slime inhibitor for circulating cooling water Download PDFInfo
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- CN104286030A CN104286030A CN201410522780.6A CN201410522780A CN104286030A CN 104286030 A CN104286030 A CN 104286030A CN 201410522780 A CN201410522780 A CN 201410522780A CN 104286030 A CN104286030 A CN 104286030A
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Abstract
The invention discloses a biological enzyme biological slime inhibitor for circulating cooling water. The biological enzyme biological slime inhibitor comprises the following biological enzyme components in parts by mass: 30-300 parts of glucose dehydrogenase, 5-40 parts of subtilisin, 5-20 parts of lysozyme, 2-20 parts of glucoamylase, 1-10 parts of lipolytic enzyme, 1-20 parts of trypsin, 1-20 parts of cellulase and 50-700 parts of water. The whole biological enzyme biological slime inhibitor disclosed by the invention can effectively inhibit the growth of biological slime deposited on the inner walls of equipment and pipelines of a circulating cooling water system and the like and further has a bactericidal function; and furthermore, the whole biological enzyme biological slime inhibitor is a pure biological preparation, has the characteristics of no toxicity, no corrosion and no pathogenicity and can prevent the problem of secondary pollution when traditional chemical medicaments are used.
Description
Technical field
The present invention relates to circulating water cooling treatment medicament, be specifically related to a kind ofly effectively to suppress foundry loam in recirculating cooling water system and the biological slime inhibitor of environmental protection.
Background technology
Biological clay be extensively be present in cooling circulating water system the inwall such as pipeline, cooling tower on and hull, underwater operation equipment surface on.Main harm is the fouling and the heavy corrosion that cause equipment, causes that heat transfer efficiency reduces, service life shortens, and operation cost increase etc., have had a strong impact on the normal operation of device, brought economic loss.Therefore, improve the technical merit controlling biological clay, reduce energy consumption, the good utilization realizing resource and equipment is the important channel that enterprise overall economic benefit improves.
In recent years the suppression of mud is attracted great attention.At present, although sticky mud inhibitor kind is a lot, its foundry loam rejection and environmental benefit still can not meet the requirement of current water treatment.Abroad to the research in sticky mud inhibitor direction mainly lay particular emphasis on development environment friendly, can biological degradation type, low cost water treatment agent.
Along with the development of bioscience, the field involved by biotechnology is also constantly widened, and adopts biological method to control the growth of biological clay, the new focus day by day become.
Existing sticky mud inhibitor mostly is the bactericide of chemosynthesis and the compound product of surfactant, as CN185373A proposes a kind of Biocidal algae-killing agent that can carry out effectively suppressing for microorganism viscosity mud, it contains isothiazolinone, dodecyl dimethyl benzyl ammonium chloride, polyacrylic acid.CN101849574A discloses a kind of efficient germicide, it is the product containing isothiazolinone, trishydroxymethylnitromethane and dibromoethyl alcohol three kinds of key components, there is during low dosage good microorganism killing and foundry loam inhibition, can apply in industrial circulating water field.The component of above-mentioned compound product is chemosynthesis medicament, has certain toxicity, all can bring the problem of secondary pollution to environment.
The method of biology enzyme is adopted to control biological clay, because it is green, the feature such as efficient more and more receives the concern of numerous researchers especially, as (Saeki D. such as Daisuke Saeki, Nagao S., Sawada I., et al.Development of antibacterial polyamide reverse osmosis membrane modified with a covalently immobilized enzyme [J] .Journal of Membrane Science, 2013, 428:403-409) by adding immobilized enzyme in polyamide reverse osmose membrane, achieve the effective control to biological clay.China has the report with ferment treatment for the control method of biological clay in circulation, but be all that single enzyme is directly soluble in water, the biological clay generated is degraded, not there is the killing action to bacterium algae, fundamentally can not prevent the generation of biological clay.
Summary of the invention
The object of the invention is to overcome the above-mentioned problems in the prior art, a kind of sticky mud inhibitor can effectively peeling off and suppress the biological clay that the e-quipment and pipe inwall of recirculating cooling water system deposits is provided.
In order to achieve the above object, the invention provides a kind of biology enzyme biological slime inhibitor for recirculated cooling water, be made up of the biology enzyme component of following mass fraction:
GDH 30-300 part,
Subtilopeptidase A 5-40 part,
Lysozyme 5-20 part,
Glucoamylase 2-20 part,
Grease catabolic enzyme 1-10 part,
Trypsase 1-20 part,
Cellulase 1-20 part,
Water 50-700 part.
Biology enzyme biological slime inhibitor of the present invention, preferably be made up of the biology enzyme component of following mass fraction: GDH 250 parts, subtilopeptidase A 20 parts, lysozyme 10 parts, glucoamylase 10 parts, grease catabolic enzyme 5 parts, 20 parts, trypsase, cellulase 10 parts, 500 parts, water.
Present invention also offers the application of above-mentioned biology enzyme biological slime inhibitor in recirculating cooling water system, the input amount of wherein said full biology enzyme biological slime inhibitor does not specially require, preferably, with the recirculated cooling water of often liter for benchmark, the input amount of described full biology enzyme biological slime inhibitor is 20-100mg, more preferably 30-50mg.
Biology enzyme biological slime inhibitor of the present invention produces H by adopting the synergy of glucoamylase, grease catabolic enzyme, trypsase cellulase, subtilopeptidase A, lysozyme, GDH
2o
2, not only decompose the generation suppressing foundry loam, and and the H that will produce
2o
2control the formation of biological clay for killing microorganisms in follow-up application.
Biology enzyme biological slime inhibitor of the present invention is compared with existing biological slime inhibitor, there is following advantage: full biology enzyme biological slime inhibitor of the present invention is pure biologic product, there is the feature of avirulence, non-corrosiveness, no pathogenicity, can not secondary pollution be caused.
Accompanying drawing explanation
Fig. 1 is the biological clay stripping off device structural representation in the embodiment of the present invention 1.
1 cooling tower in figure, 2 tanks, 3 culture tanks, 4 thermostatic control systems, 5 bubblers, 6 circulating pumps, 7 Buffer Pools, 8 testing tubes, 9 thermometers, 10 independent electric heating temperature control devices, 11 flowmeters, 12 valves.
Embodiment
Below in conjunction with accompanying drawing, biology enzyme biological slime inhibitor of the present invention and technique effect are further described.
The preparation of embodiment 1 biology enzyme biological slime inhibitor
Under room temperature condition, glucoamylase 10 parts is added successively in 500 parts of water, grease catabolic enzyme 5 parts, 20 parts, trypsase, cellulase 10 parts, subtilopeptidase A 20 parts, lysozyme 10 parts, GDH 250 parts stir entirely molten after, obtain the biology enzyme biological slime inhibitor A of translucent yellowish-brown liquid, there is enzyme distinctive smell.
Bactericidal property is evaluated
The method of evaluating performance of bactericide adopts, and [the test bottle method of plate count and dilution-to-extinction method, reference standard be the mensuration plate count of mucus formation bacterium " in the industrial circulating cooling water " (GB/T14643.1-2009), the mensuration MPN method of sulfate reducing bacteria " in the industrial circulating cooling water " (GB/T14643.5-2009), the mensuration MPN method of iron bacteria " in the industrial circulating cooling water " (GB/T14643.6-2009) and " Performance Valuation of Bactericides method " (SY/T 5890-1993).
Sulfate reducing bacteria (SRB) bacterial classification takes from northeast oil field extracted water, and bacteria suspension adopts this oil field extracted water to prepare, bacterium medicine 2h time of contact, T=45 DEG C; Heterotroph (TGB) and iron bacteria (FB) bacterial classification from chemical plant, Tianjin cycle water, the on-the-spot water preparation of this factory of bacteria suspension, time of contact 4h, T=37 DEG C.
Biology enzyme biological slime inhibitor obtained for the embodiment of the present invention 1 is carried out bactericidal property with other medicaments compare, choose the adding consistency of various medicament according to the basically identical principle of cost.Table 2 is sterilization test result.
The bactericidal property evaluation comparison of table 2 biology enzyme biological slime inhibitor of the present invention and other medicaments
Note: in (1) table 2, bactericide used: A is indication biology enzyme biological slime inhibitor of the present invention product in embodiment 1; Isothiazolinone, 14% industrial goods; DBNPA, DBNE, BNPD, DBMAL are the industrial goods of purity >=99%;
(2) the blank bacterium number of TGB, SRB, FB is followed successively by: 7.0 × 10
-7individual/mL, 2.5 × 10
-6individual/mL, 2.0 × 10
-5individual/mL.
From table 2 bacterium test result, A has very excellent bactericidal property.
Biological clay rejection is tested
The stripping performance of biology enzyme biological clay remover of the present invention adopts homemade foundry loam evaluating apparatus to test, and device schematic diagram is shown in shown in accompanying drawing 1.
Method of operating is as follows: (1) adds the cultivation that bacterial classification and nutrients carry out bacterium algae in culture tank 3; (2) after having cultivated, access stainless steel testing tube 8 (pipe range 25cm), dynamic circulation, after making stainless steel testing tube grow foundry loam to regulation requirement, takes out testing tube 8,40 DEG C of dry 2h, weighs; (3) testing tube after weighing is accessed again, in Buffer Pool 7, add medicament, run 24h, then take out test 8,40 DEG C of dry 2h, weigh.Whole Dynamic controlling parameter is: pH value=8.0 ~ 8.5, temperature 32 ± 1 DEG C, flow velocity 1.0m/h.Test result is shown in Table 3:
Table 3 biological slime inhibitor rejection of the present invention test and comparison
Prepared biological slime inhibitor product in note: A---the embodiment of the present invention 1;
GA---commercially available polyhexamethylene guanidine hydrochloride (25% effective mass content);
1227---dodecyl benzyl dimethyl ammonium chloride, 45% effective mass content;
DBNPA---solid, 99%, industrial goods;
Isothiazolinone-import industrial goods, 14% effective mass content.
From table 3 test data, under experimental concentration condition, biology enzyme biological slime inhibitor product A of the present invention is all excellent to the inhibition of biological clay than corresponding single-dose product APHG, DBNPA, isothiazolinone, also be greatly better than conventional mono-quaternaries 1227, GA and then substantially there is no inhibition.As can be seen here, biostatic agent of the present invention has more significant inhibition.
Claims (4)
1. for a biology enzyme biological slime inhibitor for recirculated cooling water, it is characterized in that, be made up of the biology enzyme component of following mass fraction:
GDH 30-300 part,
Subtilopeptidase A 5-40 part,
Lysozyme 5-20 part,
Glucoamylase 2-20 part,
Grease catabolic enzyme 1-10 part,
Trypsase 1-20 part,
Cellulase 1-20 part,
Water 50-700 part.
2. biology enzyme biological slime inhibitor according to claim 1, it is characterized in that, be made up of the biology enzyme component of following mass fraction: GDH 250 parts, subtilopeptidase A 20 parts, lysozyme 10 parts, glucoamylase 10 parts, grease catabolic enzyme 5 parts, 20 parts, trypsase, cellulase 10 parts, 500 parts, water.
3. the application of biology enzyme biological slime inhibitor in recirculating cooling water system as claimed in claim 1, it is characterized in that, with the recirculated cooling water of often liter for benchmark, the input amount of described full biology enzyme biological slime inhibitor is 20-100mg.
4. the application of full biology enzyme biological slime inhibitor according to claim 3 in recirculating cooling water system, is characterized in that, the input amount of described biology enzyme biological slime inhibitor is 30-50mg.
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| CN201410522780.6A CN104286030A (en) | 2014-09-30 | 2014-09-30 | Biological enzyme biological slime inhibitor for circulating cooling water |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105567667A (en) * | 2015-11-24 | 2016-05-11 | 威海翔宇环保科技股份有限公司 | Bio-enzyme medicament capable of controlling biological slime in circulating water |
| CN114634847A (en) * | 2020-12-16 | 2022-06-17 | 北京世城双清科技有限公司 | Bacteriostatic cleaning agent |
Citations (4)
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| CN101040052A (en) * | 2004-09-10 | 2007-09-19 | 诺维信北美公司 | Methods for preventing, removing, reducing, or disrupting biofilm |
| CN101490232A (en) * | 2006-07-24 | 2009-07-22 | 金克克国际有限公司 | Enzymatic prevention and control of biofilm |
| JP2013184073A (en) * | 2012-03-05 | 2013-09-19 | Seinen:Kk | Enzyme treatment agent |
| CN103598196A (en) * | 2013-10-25 | 2014-02-26 | 中国海洋石油总公司 | Biological slime stripping agent for water treatment, and preparation method thereof |
-
2014
- 2014-09-30 CN CN201410522780.6A patent/CN104286030A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101040052A (en) * | 2004-09-10 | 2007-09-19 | 诺维信北美公司 | Methods for preventing, removing, reducing, or disrupting biofilm |
| CN101490232A (en) * | 2006-07-24 | 2009-07-22 | 金克克国际有限公司 | Enzymatic prevention and control of biofilm |
| JP2013184073A (en) * | 2012-03-05 | 2013-09-19 | Seinen:Kk | Enzyme treatment agent |
| CN103598196A (en) * | 2013-10-25 | 2014-02-26 | 中国海洋石油总公司 | Biological slime stripping agent for water treatment, and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| DAISUKE SAEKI等: "Development of antibacterial polyamide reverse osmosis membrane modified with a covalently immobilized enzyme", 《JOURNAL OF MEMBRANE SCIENCE》, vol. 428, 30 October 2012 (2012-10-30), pages 403 - 409 * |
| 张磊: "生物酶法处理工业循环水系统中生物粘泥的研究", 《安徽化工》, vol. 40, no. 3, 30 June 2014 (2014-06-30), pages 68 - 69 * |
| 韩笑: "酶处理技术在循环冷却水系统生物粘泥控制中的应用", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》, no. 06, 15 June 2009 (2009-06-15), pages 015 - 24 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105567667A (en) * | 2015-11-24 | 2016-05-11 | 威海翔宇环保科技股份有限公司 | Bio-enzyme medicament capable of controlling biological slime in circulating water |
| CN114634847A (en) * | 2020-12-16 | 2022-06-17 | 北京世城双清科技有限公司 | Bacteriostatic cleaning agent |
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Address after: 100010 Beijing, Chaoyangmen, North Street, No. 25, No. Applicant after: China National Offshore Oil Corporation Applicant after: CNOOC TIANJIN CHEMICAL RESEARCH & DESIGN INSTITUTE CO., LTD. Applicant after: CNOOC Energy Development Co., Ltd. Address before: 100010 Beijing, Chaoyangmen, North Street, No. 25, No. Applicant before: China National Offshore Oil Corporation Applicant before: CNOOC Tianjin Chemical Research & Design Institute Applicant before: CNOOC Energy Development Co., Ltd. |
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