CN104181312B - A kind of skin free cholesterol noninvasive detection device for atherosclerosis risk assessment - Google Patents

Abstract

A kind of skin free cholesterol noninvasive detection device for atherosclerosis risk assessment. By applying particular agent to palm part, utilize optical method for measuring reaction reagent reflection spectrum changing conditions, obtain the relative content of subjects skin's free cholesterol, and then the risk that experimenter suffers from atherosclerosis class disease is assessed.

Description

A kind of skin free cholesterol noninvasive detection device for atherosclerosis risk assessment

Technical field

The present invention relates to a kind of skin free cholesterol noninvasive detection device for atherosclerosis risk assessment, it is particularly useful for the early detection of atherosclerosis.

Background technology

Along with the change of socio-economic development and people life style, atherosclerosis and the multiple cardiovascular and cerebrovascular diseases caused by it, become a big killer of human health. All accounting for first of every disease at the sickness rate of this disease of developed country and case fatality rate, and comprising the developing country of China, the morbidity of this disease and sickness rate are also in ascendant trend year by year. The many non-evident symptons of early atherosclerosis patient, even if part patient occurs that symptom but fails to go to a doctor in time, part patient is to die suddenly as First presentation, and therefore prevention seems particularly important. A large amount of epidemiology and the display of ebm evidence, atherosclerosis is a kind of controlled, the disease that can prevent. The key that effective prevention of arterial is atherosis is accurately to lock high-risk colony.

Detection blood cholesterol levels is one of main means of assessment atherosclerosis risk at present. But, in blood, the content of cholesterol is relatively big by liver function influence, and needs by blood test, for there being wound detection, can cause experimenter's health and psychological discomfort; Simultaneously, it is necessary to long-time rear blood sampling on an empty stomach, detected result food effect in examined early stage is very big; In addition, usually need to being furnished with complicated and expensive Analytical equipment, testing process is loaded down with trivial details, and the time obtaining detected result is longer. These are not enough above, hinder people and often or regularly carry out the wish of blood cholesterol levels detection, are unfavorable for that the morning of atherosclerosis class disease finds.

In addition, by the detection of the experiment of treadmill exercise load, pulse wave conduction speed detection, ankle arm exponential sum heart ankle index, can noninvasively estimating atherosclerosis risk, but there is the shortcomings such as complicated operation, detection device are expensive equally, limit it and apply.

Skin is the metabolic important place of cholesterol, and in human body, the cholesterol of about 11% is present in skin. Skin cholesterol mainly come from epidermis Steroidgenesis and from the recycle system by low-density lipoprotein (LDL) and B class 1 type street cleaner (SR-B1) by bulk diffusion. In addition, about having the cholesterol of 10%-15% to be got rid of by skin passage in estimated body, this is that the cholesterol being only second to liver removes passage. 1970, first France pathologist reported, increased with, in atherogenesis process, arterial wall can experience similar metamorphosis to skin at the age. Started from 1999, comprise multiple North America research teams of the experimental therapy system of Wisconsin medical college of university, the clinical foundation in Cleveland, medical college of Johns Hopkins University, the University of Pennsylvania, the relation of skin cholesterol and atherosclerosis, inflammation, myocardial infarction has been studied. Series of studies shows, along with the cholesterol amount in age growth, the accumulation of cholesterol in skin and arterial wall deposits is closely related, skin cholesterol can as the Novel marker of atherosclerosis. Further, in skin, food effect is little by measuring in earlier stage for cholesterol level, without the need on an empty stomach before test. Therefore, detect the atheromatosis risk information that the cholesterol spread and be stacked in skin can provide new, this information is different from traditional atherosclerosis risks and assumptions such as blood cholesterol levels, it is possible to strong as the latter supplements, and has important using value.

Conventional skin cholesterol detection is mainly realized by skin biopsy. The method can cause subject for pain when obtaining skin samples, and biopsy site has infected risk, it is necessary to professional person operates. And, biopsy samples comprises the multilayered structures such as subcutaneous lipids, and some of them are height vascularizations. Therefore, these samples contain different cholesterol sources, can not draw repetition, reliably cholesterol analysis result.

Utilize the cholesterol of solvent extraction keratoderma, as what high performance liquid chromatography and gas-chromatography and mass spectrum were used in conjunction is separated and analytical procedure. This kind of analytical procedure complexity, the more important thing is that human body skin is had corrodibility and pungency by the reagent of use, is unsuitable for experimenter's detection long-term, self-.

That mentions in Chinese patent CN1961078 gets skin exocuticle cholesterol sample with adhesive tape is sticky, realize cholesterol detection, but whole testing process needs to carry out multiple experiment, program is complicated, consuming time longer, and, owing to being difficult to ensure the repeatability of experimentation, cause detecting the cholesterol level obtained and there is difference.

US Patent No. 5489510 proposes one group of development for skin cholesterol Noninvasive detection reagent, with a kind of containing specific cholesterol in conjunction with the reagent of component and a kind of reagent containing indicator component, by the change of visible color after two kinds of reagent reacts, qualitatively judge cholesterol levels. Although the method is simple, quick, but needs professional person to be observed by subjective vision and judge, and does not draw the detected result of quantification.

US Patent No. 8236516 disclose a kind of skin cholesterol Noninvasive detection method and equipment, palm applies one group with skin cholesterol specific binding and the reagent occurring color to change, judge cholesterol level relatively by color measuring. The method is simple, quick, but, in testing process, equipment meeting different from the contact pressure of skin causes skin surface to change, and then reduces the repeatability of color measuring, and, skin is the substrate of test agent, for different experimenters, its color distortion is relatively big, and the measurement of reagent color is brought larger interference. In addition, the difference of envrionment temperature, can cause the active difference of enzyme in detection reagent, thus cause measuring error.

Summary of the invention

The technology of the present invention is dealt with problems: overcome the deficiencies in the prior art, it is provided that a kind of skin free cholesterol noninvasive detection device for atherosclerosis risk assessment, has without wound, good advantage quick, accurate, repeated.

The technology of the present invention solution: a kind of skin free cholesterol noninvasive detection device for atherosclerosis risk assessment, comprise and enough detection reagent are added drop-wise to skin selected areas, after the free cholesterol of detection reagent in skin is fully combined, utilize water sucting medium to remove excessive detection reagent, now stay the detection reagent on skin and in epiderm skin, free cholesterol exists positive correlation; Then drip to skin and add and can react with detection reagent, and produce the indicator of changes in optical properties, the degree of changes in optical properties has positive correlation with the content of detection reagent is same on skin, by measuring the diffuse reflection spectrum of the rear reagent of reaction, the degree of reflection changes in optical properties, obtain the content of free cholesterol in tested skin, and combine " skin free cholesterol-atheromatosis risk index " correlation model, finally realize the risk assessment of atherosclerosis.

Wherein, detection reagent contains the cholesterol binding compounds of a species specificity, can be combined with the free cholesterol of skin selected areas, cholesterol specificity junction mixture is connected by linking agent with a kind of enzyme, the cholesterol specificity junction mixture that enzyme labelling is crossed can either be combined by the free cholesterol in skin, can react with specific substrate again, thus indicate the content of free cholesterol in skin. Indicator is the specific substrate that can occur with enzyme in detection agent to react.

The detection device of the present invention is made up of light source part, emission element, reception parts, optical detection parts, function unit, display unit, probe component.

Light source part provided by the invention comprises photophore, luminous power, the 2nd temperature sensor and light-source temperature controller composition. Luminous power is supplied to the stable power supply of photophore, it is preferable that photophore be white light emitting diode (LED). LED is a kind of novel, energy-conservation subminiature cold light source, easily safeguards, system stability is good, and the life-span is long, covers wide waveband, is specially adapted to the needs of miniaturization instrument. Photophore can also be one or more the combination in incandescent light, halogen lamp, neon lamp. The temperature sensor output terminal being connected with photophore is connected with the input terminus of light-source temperature controller; Light-source temperature controller can keep light-source temperature constant, and then the light that light source is sent keeps stable.

The light sent by LED, irradiates reaction reagent, utilizes the reflected light receiving parts and collecting reagent and be reflected back. Preferred optical detection parts are spectrographs, spectrograph can receive the spectrum information of reflectivity with wavelength change, but not the change of the interior total light intensity of single a certain wave band, by the diffuse reflection spectrum after measurement reagent react, obtain the free cholesterol relative content of tested skin, and then the risk that experimenter suffers from atherosclerosis is assessed. Optical detection parts can also be other equipment, such as photorectifier, photomultiplier etc.

Preferred light emission and reception parts are optical fiber. The light that photophore sends by lens focus in launching fiber 2, it is mapped to reaction reagent from launching fiber illumination out, the light returned from reaction reagent reflection is passed to optical detection parts again after receiving optical fiber reception, owing to the dispersion angle of launching fiber transmitted beam is certain, only partial reaction reagent and skin can be subject to the irradiation of light beam, equally, receive optical fiber and also can only receive the light beam within the scope of certain angle; Only launching fiber range of exposures just can be detected with the light receiving the special angle scope that optical fiber range of receiving overlapping region is reflected back, determine launching fiber and receive core footpath, the numerical aperture of optical fiber, and after the shape size of reaction reagent, select launching fiber by optical simulation and receive relative angle and the relative position of optical fiber, the light beam that reception optical fiber receives only reaction reagent and is reflected back can be made, the interference that the light beam avoiding subjects skin to be reflected back causes.

Emission element can also be made up of passing through a collimating system, spectral filter, receive parts by focusing on lens, spectral filter forms. The light beam that photophore is sent by colimated light system changes into nearly parallel light such that it is able to effectively utilize light source, and avoids the interference of stray light. If stray light is more in system, the signal of stray light can affect the extraction of flashlight, even floods flashlight, reduces system looks sensitivity and accuracy. Light beam through collimation irradiates reaction reagent after mating plate first after filtration again, and this spectral filter can make the light intensity of each wavelength within the scope of measure spectrum more unified. Meanwhile, in order to make skin selected areas light beam irradiation evenly, it is possible to adopt the radiation modality that two photophores symmetry is placed. The light beam that reaction reagent is reflected back, by directly being received by optical detection parts after spectral filter, focusing lens. Spectral filter only allows the light beam changing the sensitiveest one section SPECTRAL REGION in diffuse reflection spectrum to pass through, and stops the passing through of light beam of its all band, reduces the interference of stray light. The effect focusing on lens collects more diffuse-reflectance light to reception parts, it is to increase the detection sensitivity of system. By optimization design emission element with receive the focal length of parts, relative position, relative angle, it is possible to make the light beam that reception parts receive only reaction reagent and are reflected back, avoid light beam that subjects skin is reflected back on the impact of measuring result.

Function unit is used for analyzing and processing, the record of the control of each parts and data. First the spectroscopic data that optical detection parts receive is gathered, calculation result is screened, revises and is analyzed by the temperature, the pressure information that detect in conjunction with the information such as age of experimenter of user's input, sex, height, body weight and detection device, in conjunction with " skin free cholesterol-atherosclerosis risk " correlation model, obtain subjects skin's free cholesterol contents level and suffer from the risk index of atherosclerosis, test result is carried out record, and is transferred to display unit and makes it export to user. Each measuring result can also pass through USB serial ports, realize the derivation of data, conveniently realize the statistical study of big batch data, and the long-term take off data of single user can be carried out statistical study, provide rational diet suggestion and suffer from atherosclerosis risk and assess.

" skin free cholesterol-atheromatosis risk index " correlation model, it is possible to by the statistical study of the number of subjects certificate of large sample amount is obtained. Gather the clinical indices such as experimenter's blood pressure, total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein, the temperature that detects in conjunction with age of experimenter of user's input, sex, height, body weight information and detection device, pressure information, and by free cholesterol contents level that diffuse reflection spectrum calculates, carry out regression analysis, correlation analysis, and then set up skin free cholesterol and the correlation model of atheromatosis risk index, it is determined that based on the classification thresholds of the atherosclerosis risk that skin free cholesterol is measured. After correlation model is set up, only need to gather the spectroscopic data that optical detection component receives, the temperature that detects in conjunction with the subject age of user's input, sex, height, body weight and detection device, pressure information, can obtain the risk that experimenter suffers from atherosclerosis.

The diffuse reflection spectrum of the reaction reagent that skin free cholesterol content value can be dripped on the skin samples being added in different cholesterol level by measuring obtains. The reflective light intensity of certain wave strong point depends on free cholesterol level in skin samples, therefore by analyzing diffuse reflection spectrum information, can directly obtain the relative value of testing sample free cholesterol content. The absolute value of the content of free cholesterol in sample can also be obtained in conjunction with other quantitative assay technology, utilize the free cholesterol of solvent extraction keratoderma to be measured, what employing high performance liquid chromatography was used in conjunction with gas-chromatography and mass spectrum is separated the absolute value obtaining skin free cholesterol content to be measured with analytical procedure, by repeatedly testing, set up diffuse reflection spectrum and the correlation model of skin free cholesterol content, model can directly obtain skin cholesterol levels absolute value according to diffuse reflection spectrum after setting up.

Probe apparatus provided by the invention can be fitted with the reagent trough on palm, thus launches when ensureing to measure every time and to receive parts constant relative to the position of reaction reagent. Meanwhile, in measuring process, the pressure applied by detection device due to operator is different, can cause detection device from skin apart from different, cause the change of light path, the final measurement affecting reflection spectrum. For avoiding this problem, the present invention is equipped with pressure transmitter on probe, for the contact pressure of measuring sonde parts and reagent trough, and contact pressure value is fed back to function unit, by function unit judge contact pressure whether satisfy condition and point out operator the need of adjustment contact pressure, thus contact pressure is consistent when ensureing to measure every time, avoid the measuring error caused owing to operator applies pressure difference.

Simultaneously, owing to temperature can affect the activity of enzyme in detection reagent, therefore, probe is equipped with temperature sensor simultaneously, for measures ambient temperature value, and temperature value is fed back to function unit, judge temperature whether in useful range by function unit, and according to measuring the temperature value obtained, measuring result is calibrated, reduce the measuring error that the impact of enzymic activity in reaction reagent is caused by temperature.

The present invention's useful effect compared with prior art is: the present invention proposes a kind of skin free cholesterol noninvasive detection device for atherosclerosis risk assessment, by reagent diffuse reflection spectrum on human body palm skin, realize the measurement of skin free cholesterol content, have without the need on an empty stomach, without wound, painless, be easy to operation, go out the advantages such as result fast. Device exploring block is furnished with pressure transmitter and can ensure every time measuring apparatus applying pressure is basically identical, reduce the measuring error owing to operator operates reason and causes, by designing the light emission of ad hoc structure and receive parts, avoid the interference of color distortion to measuring result of substrate skin. Skin free cholesterol detected result, utilize data processor to after subject age, sex correction, for assessment of the atherosis risk of subject artery, have without wound, quick, simple operation and other advantages, contribute to find to have good application prospect the morning of atherosclerosis high risk population.

Accompanying drawing explanation

Fig. 1 is apparatus of the present invention one-piece construction figure;

Fig. 2 is the light source part structure iron of apparatus of the present invention;

Fig. 3 is that apparatus of the present invention are launched and receive parts fiber mode structure iron;

Fig. 4 is that apparatus of the present invention are launched and receive the direct receiving mode structure iron of parts;

Fig. 5 is the structure iron of apparatus of the present invention probe component;

Fig. 6 is that detection device is for decision flowchart during atherosclerosis risk assessment.

Embodiment

As shown in Figure 1, the present invention is made up of light source part 1, emission element 2, reception parts 3, optical detection parts 4, function unit 5, display unit 6 and probe component 7; Described function unit 5 is electrically connected with optical detection parts 4; Described display unit 6 is electrically connected with function unit 5; Described probe component 7 is electrically connected with function unit 5;

As shown in Figure 2, the light source part of the present invention comprises photophore 11, luminous power 12, the 2nd temperature sensor 13 and light-source temperature controller 14; Luminous power 12 is electrically connected with photophore 11,2nd temperature sensor 13 output terminal is electrically connected with light-source temperature controller 14 input terminus, described light-source temperature controller 14 output terminal is connected with photophore 11, described light-source temperature controller 14 can keep photophore 11 homo(io)thermism, and then the light that photophore 11 is sent keeps stable; Luminous power 12 is electrically connected with function unit 5;

As shown in Figure 3, emission element 2 and reception parts 3 are made up of optical fiber, the light that photophore 11 sends by lens focus in launching fiber 21, it is mapped to reaction reagent 9 from launching fiber 21 illumination out, reflect the light returned from reaction reagent 9 and it is passed to optical detection parts 4 again after receiving optical fiber 31 reception, only launching fiber 21 range of exposures just can be detected with the light receiving the special angle scope that optical fiber 31 range of receiving overlapping region is reflected back, by optimization design launching fiber 21 and the relative angle and the relative position that receive optical fiber 31, the light beam that reception optical fiber 31 receives only reaction reagent 9 and is reflected back can be made, the interference that the light beam avoiding subjects skin to be reflected back causes.

As shown in Figure 4, emission element 2 by the first passing through a collimating system 22,23, spectral filter 24, the 2nd passing through a collimating system 25,26 and spectral filter 27 form; Receive parts 3 to be made up of focusing lens 32, spectral filter 33; The light beam that photophore 11 sends is changed into nearly parallel light by the first passing through a collimating system 22,23, and nearly parallel light irradiates reaction reagent 9 after mating plate 24 after filtration again; The light that reaction reagent 9 is reflected back, by being measured by optical detection parts 4 after spectral filter 33, focusing lens 32. By optimization design emission element 2 with receive the focal length of parts 3, relative position, relative angle, it is possible to make the light beam that reception parts 3 receive only reaction reagent 9 and are reflected back, avoid light beam that subjects skin is reflected back on the impact of measuring result.

As shown in Figure 5, the probe component 7 of the present invention is made up of support 71, first temperature sensor 72, pressure transmitter 73 and detection hole 74, detection hole 74 is positioned at support 71 central position, run through whole support 71, first temperature sensor 72, pressure transmitter 73 are embedded on support 71, lay respectively at detection both sides, hole 74, and it is electrically connected with function unit 5.

As shown in Figure 6, when skin cholesterol is measured by the present invention, judging whether measuring tempeature satisfies condition by function unit 5, if condition does not meet, waiting temperature condition is measured after meeting again; If satisfying condition, judging whether pressure satisfies condition, if not meeting measuring condition, prompting tester changes pressure, to satisfy condition; Temperature and pressure start to measure when all satisfying condition, and after acquisition spectroscopic data is processed by data processor, result of determination, whether higher than threshold value, if higher than threshold value, is judged to high risk population, if lower than threshold value, is then judged to normal population.

Embodiment 1

Cholesterol specificity junction mixture in detection reagent, enzyme, linking agent are respectively the polymkeric substance of purple foxglove soap glycosides, horseradish peroxidase and maleic anhydride-NVP, and in indicator, main component is 3,3', 5,5'-tetramethyl benzidine. First, cleaning experimenter's palm skin, after xerosis cutis, drips and adds detection reagent to palm detected part. Through about 1 minute hatch, blot test zone, to remove the detection reagent not being combined with free cholesterol. Then, dripping at test zone and add 3,3', 5,5'-tetramethyl benzidine, react with the horseradish peroxidase in detection reagent, in the purple foxglove soap glycosides of level of response and combination and skin, cholesterol level is directly proportional. Again through 2 minutes, utilize the diffuse reflection spectrum of detection measurement device reaction reagent.

Detection device is made up of light source part, emission element, reception parts, optical detection parts, function unit, display unit and probe component. Probe apparatus structure as shown in Figure 1, is made up of support 71, temperature sensor 72, pressure transmitter 73 and detection hole 74. Support is used for the fixing of device, and temperature sensor 72 is for measuring tempeature. The temperature sensor 72 used in this probe component is DS18B20. The data gathered show, reagent is just to reach good reaction effect in 25-37 DEG C of environs in room temperature, therefore, limit and only provide detected result to being in the experimenter within this scope, cannot ensure when exceeding this scope that data are effective, and according to measuring tempeature value, result is revised, reduce the measuring error that effect of temperature causes.

Contact pressure reflects probe component 7 and the degree of human skin's laminating. Pressure can cause the distance between light source and skin, detector and skin to change, thus changes light path, and then changes measuring result. The pressure transmitter 73 used in this detection device is capacitive pressure sensor, makes operator change hand by software interface prompting and applies pressure, ensures that probe pressure is 0.9-1.1N, cannot ensure that data are effective when exceeding this scope.

When the present invention uses, the light sent by white light LEDs is through lens focus in launching fiber 21, and the light that launching fiber sends is irradiated in reaction reagent by detection hole 74, and the light beam that reaction reagent is reflected back, by receiving after optical fiber 31 receives, is transferred in spectrograph. By optimization design launching fiber with receive the relative angle of optical fiber and position, it is possible to make the spectrum information that reception optical fiber is only collected reaction reagent and is reflected back, thus reduce the metrical error owing to subjects skin's color distortion causes. In the present embodiment, the core footpath of transmitting and reception optical fiber is 500 ��m, and numerical aperture is 0.22. The incident angle of launching fiber is 45 ��, and the distance to skin surface is 15mm; The receiving angle receiving optical fiber is 30 ��, and the distance to skin surface is 15mm, ensure that the light beam that reception optical fiber receives mainly comes from the transmitting of reaction reagent.

Receive optical fiber directly to transport light in spectrograph, then process by analysis process system. In treating processes, system is to temperature, pressure, it is determined that the reasonableness of image data, then by Data Analysis Services, judges the height of subjects skin's free cholesterol content, analyzes the risk level that experimenter suffers from atherosclerosis.

Embodiment 2

In the present embodiment, when building emission element and receive parts, set of lenses is adopted to substitute optical fiber. Light source is white light LEDs, the light that LED sends is irradiated to the skin selected areas scribbling reagent after the emission element being made up of collimation lens set and spectral filter, the reflected light in this region is after the reception parts being made up of spectral filter, lens, being received by optical detection parts, optical detection parts are micro spectrometer herein. By optimization design emission element with receive the focal length of parts, relative position, relative angle, it is possible to make the light beam that reception parts receive only reaction reagent and are reflected back, avoid light beam that subjects skin is reflected back on the impact of measuring result. After the spectrum information that spectrograph is received by analysis process system carries out analyzing and processing, export free cholesterol level value in skin, obtain the information of the atherosis risk of subject artery.

Non-elaborated part of the present invention belongs to techniques well known.

The above; being only part embodiment of the present invention, but protection scope of the present invention is not limited thereto, any those skilled in the art are in the technical scope that the present invention discloses; the change that can expect easily or replacement, all should be encompassed within protection scope of the present invention.

Claims (5)

1. the skin free cholesterol noninvasive detection device for atherosclerosis risk assessment, it is characterised in that comprising: light source part (1), emission element (2), reception parts (3), optical detection parts (4), function unit (5), display unit (6) and probe component (7), described light source part (1) comprises photophore (11), luminous power (12), the 2nd temperature sensor (13) and light-source temperature controller (14), luminous power (12) is electrically connected with photophore (11), 2nd temperature sensor (13) output terminal is electrically connected with light-source temperature controller (14) input terminus, described light-source temperature controller (14) output terminal is connected with photophore (11), described light-source temperature controller (14) can keep photophore (11) homo(io)thermism, and then the light that photophore (11) is sent keeps stable, luminous power (12) is electrically connected with function unit (5), described optical detection parts (4) are electrically connected with reception parts (3), described function unit (5) is electrically connected with optical detection parts (4), described display unit (6) is electrically connected with function unit (5), described probe component (7) is by support (71), first temperature sensor (72), pressure transmitter (73) and detection hole (74) composition, during use, probe component (7) to be fitted with the reagent trough (8) on palm, when ensureing to measure, emission element (2) and reception parts (3) are constant relative to the position of reaction reagent (9) every time, detection hole (74) is positioned at support (71) central position, run through whole support (71), first temperature sensor (72), pressure transmitter (73) is embedded on support (71), lay respectively at detection hole (74) both sides, and be electrically connected with function unit (5),

In use procedure, first, by the age of display unit (6) typing experimenter, sex, height and body weight information, and it is sent to function unit (5), reagent trough (8) is pasted on experimenter's palm skin, and the reagent adding and can occurring with skin cholesterol to react is dripped to reagent trough, again support (71) is placed on reagent trough (8), makes the first temperature sensor (72), pressure transmitter (73) and reagent trough surface contact, first temperature sensor (72) and pressure transmitter (73) feedback environment temperature, contact pressure is to function unit (5), judge whether contact pressure and envrionment temperature satisfy condition by function unit (5), prompting operator is the need of adjustment contact pressure, thus contact pressure is consistent when ensureing to measure every time, avoid the measuring error caused owing to operator applies pressure difference, and according to measuring the ambient temperature value obtained, free cholesterol detected result is corrected, reduce the measuring error because the impact of enzymic activity in reaction reagent is caused by envrionment temperature, afterwards, function unit (5) control luminous power (12) makes photophore (11) luminous, the light that emission element (2) transmission photophore (11) sends, it is radiated on reaction reagent (9), after the received parts (3) of the diffuse-reflectance light of reaction reagent (9) are collected, it is converted into electrical signal through optical detection parts (4), and transfers to function unit (5), function unit (5) is according to the diffuse-reflectance light data of the reaction reagent (9) obtained, obtain skin free cholesterol content value, and subject physiologic's information and skin free cholesterol content value are input to " skin free cholesterol-atheromatosis risk index " correlation model, obtain the risk index that experimenter suffers from atherosclerosis, atheromatosis risk index is transferred to display unit (6) by function unit (5), and shows detected result by display unit (6), wherein " skin free cholesterol-atheromatosis risk index " correlation model, is obtained by the data gathering of large sample amount, analyzing and processing in clinical trial,

Described optical detection parts are spectrographs, spectrograph can receive the spectrum information of reflectivity with wavelength change, but not the change of the interior total light intensity of single a certain wave band, by the diffuse reflection spectrum after measurement reagent react, obtain the free cholesterol relative content of tested skin, and then the risk that experimenter suffers from atherosclerosis is assessed;

Described pressure transmitter (73) is capacitive pressure sensor, makes operator change hand by software interface prompting and applies pressure, ensures that probe pressure is 0.9-1.1N, cannot ensure that data are effective when exceeding this scope;

Described emission element (2), reception parts (3) adopt launching fiber and receive optical fiber, the core footpath of launching fiber and reception optical fiber is 500 ��m, numerical aperture is 0.22, and the incident angle of launching fiber is 45 ��, and the distance to skin surface is 15mm; The receiving angle receiving optical fiber is 30 ��, and the distance to skin surface is 15mm, ensure that the light beam that reception optical fiber receives mainly comes from the reflection of reaction reagent.

2. the skin free cholesterol noninvasive detection device for atherosclerosis risk assessment according to claim 1, it is characterised in that: described photophore (11) is white light emitting diode (LED); Or it is one or more the combination in incandescent light, halogen lamp, neon lamp.

3. the skin free cholesterol noninvasive detection device for atherosclerosis risk assessment according to claim 1, it is characterized in that: the light that described photophore (11) sends by lens focus in launching fiber (21), it is mapped to reaction reagent (9) from launching fiber (21) illumination out, the light returned from reaction reagent (9) reflection is passed to optical detection parts (4) again after receiving optical fiber (31) reception, the dispersion angle of the light beam launched due to launching fiber (21) is certain, only partial reaction reagent and skin can receive the irradiation of light beam, equally, receive optical fiber (31) and also can only receive the light beam within the scope of certain angle, only launching fiber (21) range of exposures just can be detected with the light receiving the special angle scope that optical fiber (31) range of receiving overlapping region is reflected back, by optimization design launching fiber (21) and the relative angle and the relative position that receive optical fiber (31), can making the light beam that reception optical fiber (31) receives only reaction reagent (9) and is reflected back, the light beam avoiding subjects skin to be reflected back affects measuring result.

4. the skin free cholesterol noninvasive detection device for atherosclerosis risk assessment according to claim 1, it is characterized in that: in described function unit (5), " skin free cholesterol-atheromatosis risk index " correlation model is by obtaining the statistical study of the number of subjects certificate of large sample amount, it is implemented as: in conjunction with the skin free cholesterol level of experimenter, measure the contact pressure obtained, ambient temperature information, the age of the experimenter of user's input, sex, height, body weight information, and the experimenter's blood pressure gathered, total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein clinical indices, carry out regression analysis, correlation analysis, and then set up skin free cholesterol and the correlation model of atheromatosis risk index, determine the classification thresholds of the atherosclerosis risk measured based on skin free cholesterol, after correlation model is set up, only need to gather the spectroscopic data that optical detection parts receive, in conjunction with subject age, sex, height, body weight and temperature, pressure information that user inputs, the risk that experimenter suffers from atherosclerosis can be obtained.

5. the skin free cholesterol noninvasive detection device for atherosclerosis risk assessment according to claim 4, it is characterised in that: described skin free cholesterol, horizontally through the diffuse reflection spectrum measuring the upper reaction reagent of subjects skin, directly obtains; Or the absolute value of free cholesterol level in sample is obtained in conjunction with being separated of being used in conjunction of high performance liquid chromatography and gas-chromatography and mass spectrum and analysis technology, thus set up diffuse reflection spectrum and the correlation model of skin free cholesterol content, model can obtain skin cholesterol levels absolute value according to diffuse reflection spectrum after setting up.