CN104181296A - Reference timing analysis method in colloidal gold test paper quantitative analysis - Google Patents
Reference timing analysis method in colloidal gold test paper quantitative analysis Download PDFInfo
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
A reference timing analysis method in colloidal gold test paper quantitative analysis is used for providing an accurate and reliable analysis moment for the colloidal gold test paper quantitative analysis. A sample solution is dripped into a sample adding region of a test strip, and color changes of detection lines are constantly monitored by a colloidal gold test paper quantitative analysis device. With the passage of time, a T line firstly appears, and then a C line appears with a little time. Colors of the two lines are gradually deepened with the passage of time. When the concentration of the C line reaches a set threshold (v1), the gray value (v2) of the T line is began to be detected and is used as a detection result. Compared with a method of detection after a fixed time after the sample solution is dripped, detection at the time provided by the invention can exclude influence of different volumes of the added sample solution, different temperature and humidity conditions and other different conditions, and thus the stability, repeatability and reliability of the detection result are improved, and the detection precision is improved.
Description
Technical field
The present invention relates to colloidal gold strip quantitative test field, be specifically related in a kind of colloid gold test paper quantitative test with reference to timing analysis method.
Background technology
Immuno-gold labeling technology (immunogold labeling technique, ILT) be after the initial stage eighties 19th century, fluorescence immunoassay mark immune labeled continue enzyme, the large labelling technique of radioactive isotope immune labeled three, the novel solid phase labelling immunoassay growing up.The advantage that there is easy, rapid, accurate, high specificity, sensitivity is high, pollution-free, false positive rate is low etc.The ultimate principle of colloidal gold immunity chromatography is: by capillary siphoning effect, make sample and be sprayed on colloid gold label thing on glass fibre and mix on nitrocellulose membrane and move, if contain antigen (or antibody) in sample, antibody (or antigen) combination with colloid gold label, diffuse on film detection zone again with unlabelled antibody (or antigen) combination, form gold mark antigen antibody complex, be trapped on detection line and colour developing, can obtain testing result intuitively.
On colloidal gold strip, generally there are two lines, detection line (being called for short T line) and control line (being called for short C line).General coated on T line is another specific antibody of determinand, and on C line general coated be the antibody of the acceptor that on gold-marking binding pad, colloid gold label is crossed.Whether C line is effective for detection of this test, if do not observe color on line, be quite look redness or the purple of collaurum, represent this test invalidation, the result on line is inadvisable, if observe color on meaningless line, according to the color on T line, carry out result of determination.
Colloidal gold immunochromatographimethod technology is mainly used in needing the occasion of qualitative detection.When the concentration of sample surpasses certain scope in solution to be checked, there will be obvious change color, otherwise do not have.This technology particularly suitable, in the middle of the qualitative monitoring of disease, has in serum analysis, urine and there is no the abnormal material raising, the content of HCG etc. in urine during such as pregnancy.Whether this method is simply to surpass the positive boundary of the normal reference value upper limit, and testing result is for being or not being this two-value relation.
Progress along with production technology, the quality control of the NC film in colloidal gold strip is more and more stricter, the manufacturers of a lot of specialties (as Kinma-tic Automation company etc.) provide accurate liquid feeding, cutting and assembling equipment, but also the production line of a robotization complete set is provided, therefore under the condition of carrying out strict quality control, can produce character basically identical immune colloid gold gold diafiltration kit and colloidal gold immunochromatographimethod test-strips.Basic guarantee the sample that joins during each detect can and gold mark bond mix along the homogeneity advancing on NC film and the consistance of speed, make to come the concentration of interpretation sample to be called possibility according to the shade of T line.
Naked eyes interpretation is mainly to compare with standard color comparison card, has detection speed slow, the distinct disadvantage that automaticity is low.And testing staff's subjectivity is large, cannot guarantee that testing result is always safe and effective, error rate is large.The method of utilizing equipment to carry out quantitative test mainly contains: photoresistance measurement scheme, mirror based fiber optica sensor measurement reflective light intensity scheme, imageing sensor gather image scheme.Utilizing imageing sensor to gather picture signal and carry out analyzing and processing, is popular method in recent years in immunochromatography checkout equipment.CCD and CMOS are two kinds of semiconductor image sensors that generally adopt now, and they have the response time very fast, very high sampling precision, and also the interference being subject to during sampling is less, can well guarantee to gather the quality of image.Utilize image processing method to demarcate T line region, then detect the gray scale in this region, utilizing the anti-concentration that pushes away sample solution of gray-scale value is current main stream approach.
When colloidal gold reaction occurs in test strips to occur that after colour developing phenomenon, the color depth of p-wire changes over time, is generally to deepen gradually.Very crucial in order to guarantee the measurement that the accuracy of test result carries out gray-scale value at a consistent time point.After but sample solution drips in sample pad, under the effect of adsorptive pads, along NC film, move, adding under the sample liquid of different volumes, different temperature and humidity conditions and other different conditions, the speed that liquid moves is different.If so after selecting to splash into the special time after sample liquid, analyze, can not accurate description analysis result.
Summary of the invention
The object of the present invention is to provide in a kind of colloid gold test paper quantitative test with reference to timing analysis method, for colloid gold test paper quantitative test, select a time point accurately and reliably, guarantee repeatability and the accuracy of each analysis to measure.
The invention provides in a kind of colloid gold test paper quantitative test with reference to timing analysis method, its content is:
In sample liquid, splash into after test strips sample application zone, with the monitor and detection line color variation constantly of colloid gold test paper quantitative analytical device.First there is T line in passing in time, then occurs after a time C line.Article two, the color of line all passing in time deepen gradually.When the concentration of C line arrives a setting threshold (v1), start to detect the gray-scale value (v2) of T line, and as testing result.Situation about losing efficacy for test strips, sets a larger stand-by period t0, and when surpassing stand-by period t0, C line concentration does not still reach threshold value v1, thinks that C line does not occur, this time testing result is invalid.
The selected of setting threshold (v1) should be determined according to many experiments interpretation of result, selectes a suitable threshold value very important to measurement result.
Method provided by the invention is that colloid gold test paper quantitative test has been selected one with reference to constantly, at this, constantly detect to get rid of and add that sample liquid volume is different, temperature and humidity conditions is different and the impact of other different conditions, improve stability, repeatability and the reliability of testing result, improve accuracy of detection.
Accompanying drawing explanation
Fig. 1 is colloidal gold strip structural drawing.
Fig. 2 is ELISA test strip line gray scale temporal evolution curve.
Embodiment
Below in conjunction with accompanying drawing, say concrete implementation.
Fig. 1 is colloidal gold strip structural drawing, and in figure, 1 is sample holes, the 2nd, T line, the 3rd, C line.T line claims again detection line, and C line is called again nature controlling line.When C line does not develop the color, represent that testing result is invalid, being generally that test strips is expired or storage condition is improper causes.When C line has colour developing, represent that this detects effectively, the gray scale of T line colour developing color is just relevant to the concentration of sample liquid, represents the degree that colloidal gold reaction carries out.The concentration that can instead push away sample liquid according to the gray-scale value of T line color, colloid gold test paper quantitative analysis instrument is according to this principle design.
Fig. 2 is ELISA test strip line gray scale temporal evolution curve, and transverse axis is the time, and the longitudinal axis is the color gray scale of C line and T line.When sample liquid splashes into the sample holes of test strips, start timing, think to be the zero point of time shaft this moment.Along with sample liquid flows on the NC of test strips film, at t1 constantly, first T line occurs.Then spent a period of time, at t2 constantly, C line starts to occur.Along with passage of time, the gray-scale value of C line and T line is all increasing.When the gray-scale value of C line reaches setting threshold v1, the time this moment of recording is t3.The gray-scale value v2 that T line records when time t3 is exactly the testing result needing.After surpassing a larger moment t0, C line concentration does not still reach threshold value v1, thinks that C line does not occur, this time testing result is invalid, can get rid of the impact that inefficacy test strips causes this method.
Claims (1)
- In colloid gold test paper quantitative test with reference to a timing analysis method, after sample liquid splashes into the sample holes of test strips, start to monitor the grey scale change of C line and T line; It is characterized in that, at the gray-scale value of C line, reach the moment of setting threshold (v1), start to detect the gray-scale value (v2) of T line, and as testing result.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113358637A (en) * | 2021-05-11 | 2021-09-07 | 棒糖科技(杭州)股份有限公司 | Method for interpreting semi-quantitative test paper result through stabilization time |
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Application publication date: 20141203 |