CN104173047B - Networked behavior electrophysiological experiment system through collaboration of multiple computers - Google Patents
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Abstract
The invention provides a behavior electrophysiological experiment system. The system comprises one or more behavior electrophysiological experiment units, a calculation control server and a database server, wherein each behavior electrophysiological experiment unit comprises a main-control computer and controlled experiment equipment, and each main-control computer controls the controlled experiment equipment to give stimulation signals to an experimental animal and to collect and record the behavior information and the electrophysiological experiment data of the experimental animal in real-time; the calculation control server and the main-control computers are connected through a main-control local area network, and receives the behavior information and the electrophysiological experiment data transmitted by the main-control computers and conducts analysis and calculation; the database server is connected with the calculation control server through the main-control local area network, and the database server is used for storing the data and information transmitted by the calculation control server. According to the behavior electrophysiological experiment system provided by the invention, the accuracy of the behavior electrophysiological experiment can be improved effectively, high efficiency is achieved, and the immediacy in monitoring and management can be improved effectively.
Description
Technical field
The cubic network that the present invention relates to the use of multiple computers composition carries out system and its correlation of behavior electro physiology experiment
Method, belong to neuroscience field.
Background technology
Modern behavior electro physiology laboratory is generally used for computer, and its automaticity greatly carries than tradition
High.But common behavior electro physiology experiment is to control several experiments to set by a computer by the method (pseudo- real-time) of cpu timesharing
For it is impossible to realize the real function of giving kinds of experiments stimulation and collection kinds of experiments data in real time.
For example, when giving animal light and sonic stimulation record animal behavior video recording (25 frames/second, 640 × 480 resolution,
Color depth 24bit) and the multichannel brain signal of telecommunication (sample frequency is 1khz/ passage, and port number is typically more than 10), such as
Fruit takes into account data acquisition and/or data analysiss (referring to figure with a computer (whether multinuclear cpu) Control release equipment simultaneously
Three), usual Video data all can have the situation of frame (cpu core is less or dominant frequency is lower or internal memory is less, then fall frame phenomenon tighter
Weight), or simultaneously with the EEG signals short time leakage adopt (the leakage amount of adopting depend on analog digital conversion plate (ad plate) card on storage size with
And cpu reads the speed of data on ad plate).
Another problem is, if will study between EEG signals difference passage and synchronization between brain electricity and animal behavior
Problem, only can lead to the delay of the delay data collection of transmission between various signals with a computer controling equipment and sampling,
This delay can lead to the result of data analysiss to produce inestimable Systematic Errors.
Furthermore, traditional behavior electro physiology experimental system is merely able to just may be used after a few hours or a couple of days after the completion of experiment
Know the result of experiment.If if experimental strategy needs to revise research approach in time according to experimental result, such method efficiency
Just than relatively low.
Finally, in traditional behavior electro physiology is tested, if laboratory director it should be understood that laboratory internal each
The situation that experiment group current experiment is carried out, can install monitoring video additional in every experimental considerations unit, but this is merely able to see now
, and do not know that the situation that experiment is specifically carried out, for example, whether experimental result meets the expected, operation of experimenter
Whether wrong, etc..
Therefore, in order to improve accuracy and the conventional efficient of the experiment of behavior electrophysiology, strengthen the supervision to experiment, having must
To adopt new experimental system and method, to make up the defect of aforementioned conventional method.
Content of the invention
It is an object of the invention to: for overcoming the defect of above-mentioned traditional method, it is a kind of accurate, efficient that the present invention provides, with
And it is easy to behavior electro physiology experimental system and the method immediately supervised.The present invention utilizes computer network and calculates control service
Device, and coordinate corresponding software, systematically tissue multiple stage computers constitute mutually collaborative behavior electro physiology experimental system, with
The accuracy of raising behavior electro physiology experiment, high efficiency, and the instantaneity of supervision.
The invention provides a kind of behavior electro physiology experimental system, comprising: one or more behavior electro physiology experimental considerations units,
Calculate and control server and database server.Wherein, described behavior electro physiology experimental considerations unit includes a main control computer
With controlled experiment equipment, described main control computer controls described controlled experiment equipment, to give laboratory animal stimulus signal and real
When collection and record behavioural information and the electro physiology experimental data of described laboratory animal.Described calculating controls server and described master
Control computer is connected by master control LAN, receives the described experimental data of described main control computer transmission and is analyzed
And calculating.Described database server controls server to be connected by master control LAN with described calculating, in order to store meter
Calculate data and the information that control server transmits.
Present invention also offers a kind of behavior electro physiology experimental system, described behavior electro physiology experimental considerations unit also includes one
Or multiple stage working computer, described main control computer controls described by way of LAN in unit and/or serial ports are direct-connected
Working computer, controls described experimental facilitiess to give described laboratory animal stimulus signal, to adopt in real time by described working computer
Collect the described experimental data of described laboratory animal and described experimental data is sent back described main control computer.Preferably, described
Behavior electro physiology experimental considerations unit also includes network monitoring video camera, and described network monitoring video camera is by described master control LAN
Server is controlled to be connected with described calculating.
On the other hand, present invention additionally comprises the behavior electro physiology experiment software write of a set of unification, described experiment software is extremely
Few inclusion: test control module in real time, be arranged on described main control computer and described working computer, be responsible for controlling described reality
Equipment of testing is carried out behavioral experiment in real time and is gathered described experimental data, and described in collecting in the gap of real-time experimental duties
Experimental data sends described calculating to and controls server；Data reception module, is arranged on described calculating and controls on server, be responsible for
Respond the request of described main control computer in each described experimental considerations unit, receive the described experimental data that it sends over should
Experimental data is stored in the described memorizer calculating and controlling server, simultaneously according to its computation requests, by these request transmission
To parallelization data computation analysis module, make data timely be calculated analysis, finally will calculate the result transmission of analysis again
Described main control computer to each described experimental considerations unit；Described parallelization data computation analysis module, is arranged on described calculating and controls
In control server, there are multiple computation modules relevant with behavior electro physiology, withered according to the request of described main control computer and use phase
The assembly answered completes to calculate；And data transmitting module, it is arranged on described calculating and controls on server, be responsible for need long Kubo
The video recording of the animal information deposited, described experimental data and (/ or) experiment scene is sent on described database server.
Preferably, the behavior electro physiology experiment software that described unification is write also includes: image receiver module, and this image receives
Module be arranged on described calculate control server on, be responsible for receive experimental considerations unit in network monitoring video camera image data and
The described experiment scene calculating in the corresponding experimental considerations unit of real-time display on the interface controlling server.
In an embodiment of the present invention, described calculating controls server to be multiple-core server or minicomputer.
The invention provides a kind of accurate, efficient, and it is easy to behavior electro physiology experimental system and the method immediately supervised,
Have the advantages that the present invention utilizes computer network and calculates control server, and coordinate corresponding software, system
Ground tissue multiple stage computers constitute mutually collaborative behavior electro physiology experimental system, to improve the accurate of behavior electro physiology experiment
Property, high efficiency, and supervision instantaneity.
Brief description
Fig. 1 is the hardware connection diagram of the present invention.
Fig. 2 is the flow of information data flow graph of the present invention, and software function module figure.
Fig. 3 is the hardware connection diagram of traditional method.
Specific embodiment
With reference to the accompanying drawings and examples, describe the present invention in detail.
It is a kind of accurate, efficient that the present invention provides, and is easy to behavior electro physiology experimental system and the method immediately supervised.This
Invention using computer network and calculates control server, and coordinates corresponding software, systematically organizes multiple stage computers structure
Become mutually collaborative behavior electro physiology experimental system, to improve accuracy, the high efficiency of the experiment of behavior electro physiology, and supervision
Instantaneity.
Behavior electrophysiology field is very high for the requirement of real-time of experiment, mainly in the following aspects: first,
The concurrency of brain nervous cell work requires research worker must have foot for the collection of brain electric information and animal behavior information
Enough fast speed is that is to say, that the synchronicity of signals collecting to be ensured, real-time.For example, for ensureing synchronicity, need brain electricity
The error control of each passage of signal within 1ms, the time interval controls of limb motion signal within 10ms, video signal
The Separation control of every interframe is within 20ms etc.；Secondly, for researcher, interpretation speed is faster, more favourable
The experimental result of Yu research worker is made adjustment in time, so that the efficiency of experiment improves, that is, experiment management and control is real-time
Property.Although different behavior electro physiology experiments may be using different strategies, as a rule, the result of data analysiss is preferably
Enough obtain within a few minutes and show；Finally, the real-time of laboratory room managing.If laboratory director is it should be understood that reality
Test the situation that each experiment group current experiment of chamber interior is carried out, traditional method is to install monitoring additional in every experimental considerations unit to take the photograph
Camera, but this is merely able to see field condition and does not know the situation that experiment is specifically carried out, for example, whether experimental result meets
Whether expected, experimenter operation is wrong, etc..Therefore, if laboratory director can not enter each experimental considerations unit
It is known that the concrete condition that experiment is carried out, can be helpful for the management of experiment.
Behavior electro physiology experimental system involved in the present invention is made up of related hardware components and software section.According to reality
Test the difference of purpose, the hardware components of this system can comprise one or more behavior electro physiology experimental considerations units, wherein each row
Hardware configuration for electro physiology experimental considerations unit can be incomplete same, as shown in figure.
In general, each unit in such system all includes: a main control computer (host), one or many
Platform controlled device (computer display, eeg amplifier, loudspeaker etc.), and connect the master control LAN of main control computer
(s-net)；Alternatively, these units can also include: unit local area network (i-net), and one or multiple stage
Working computer (worker).
In the present invention, either calculate the master control LAN controlling server to connect each unit main control computer, or
Unit local area network, is all the LAN known to those skilled in the art.But the feature of master control LAN is speed
Hurry up, data traffic big (network speed at least more than gigabit)；And the feature of each unit local area network is speed, data
Flow less (even can be 100,000,000 be 10,000,000 network speeds).The bandwidth of master control LAN allows for meeting experimental considerations unit data
Transmission and analysis result return the needs of speed it is also necessary to ensure that experiment monitoring video recording does not produce the delay more than 2 seconds；And unit
The bandwidth of local area network must assure that equipment controls data transmission speed all within the range of error that experiment allows.For
Meet these requirements, the main control computer within unit must be respectively connected to master control LAN and list by least two pieces network interface cards
First local area network.
If an experimental considerations unit does not have working computer, by all experimental facilitiess of main control computer master control and collection institute
There is experimental signal.If there are working computer, then main control computer is by the direct-connected side of unit local area network and/or serial ports
Formula controls working computer, and wherein only one working computer passes through unit local area network (i-net) and serial ports simultaneously
The direct-connected control accepting main control computer, remaining working computer then accepts control by unit local area network.By working
Computer gives the motion letter of laboratory animal stimulus signal, the behavior electric physiological data of Real-time Collection animal, recording laboratory animal
Breath and behavior are recorded a video and according to animal behavior, animal are imposed with rewards and punishments etc..In one embodiment of the invention, make
For auxiliary, main control computer can also control device and gathered data.Generally, in the experiment of behavior electro physiology, one complete
Experiment is made up of test many times, in the short time interval (typically several seconds) after each test terminates, main control computer
Automatically by obtaining from working computer or from the experimental data itself directly obtaining pass through at a high speed, high-throughout s-net to
Calculate and control the data results that server (c-server) transmission data the reception server transmit.
In the experiment of behavior electro physiology, above-mentioned hardware configuration needs the feature according to the experiment of behavior electro physiology, via correlation
Software is unified.This is needed write unified behavior electro physiology experimental framework software installation in calculating control server and master control meter
On calculation machine, and it is directed to the different modules of different experiment contents interpolations, personnel selection for deliberation on this basis.Above-mentioned experiment
The writing of Framework, can utilize familiar computer language by those skilled in the art completely, for example, c, c++, pascal,
BASIC etc., experimental concrete needs are completing.
According to one embodiment of the invention, as shown in Figure 2, each behavior electro physiology experimental considerations unit controls with calculating
Server (c-server, multiple-core server or minicomputer) passes through (the above speed of gigabit), high-throughout master control local at a high speed
Network (s-net) is connected；Additionally, calculate controlling server also by s-net and special storage experimental data and monitoring
The database server (d-server) of information is connected, for a large amount of or long-term data access.According to the present invention one
Specific embodiments, also comprise network monitoring video camera in each behavior electro physiology experimental considerations unit, described network monitoring video camera
Server can be controlled by master control LAN to be connected with calculating, in order to laboratory director, each experimental considerations unit be regarded
Frequency monitors.
Calculate and control server to include many functional modules, such as data reception module, parallelization data calculate analysis
Module, image receiver module and data transmitting module etc..Its function is illustrated below:
Data reception module: be responsible for responding the request of main control computer in each experimental considerations unit, receive what it sended over
This experimental data is simultaneously stored in the memorizer calculating control server by experimental data；Calculating according to main control computer simultaneously
Request, these requests is sent to parallelization data computation analysis module, makes data timely be calculated analysis, finally will again
The result calculating analysis sends the main control computer of each experimental considerations unit to.In order to allow laboratory director understand entering in real time of experiment
Exhibition, the result of data analysiss can calculated on the display interface controlling server with simultaneous display.
Parallelization data computation analysis module: be responsible for the experimental data receiving and computation requests are carried out at classified counting
Reason, and gone back by data reception module transmission calculating the result after analysis.
Image receives and display module: it is responsible for receiving the image data of network monitoring video camera in experimental considerations unit, and
Calculate and the experiment scene showing in corresponding experimental considerations unit in real time is controlled on server interface, be beneficial to laboratory director monitoring each
The progress of experiment in individual experimental considerations unit.These image datas can decide whether that being stored in calculating controls service as needed
On device.
Data transmitting module: the task of this module is needing the permanent animal information preserving, experimental data and (/ or)
The video recording of experiment scene is sent to independent of calculating the place of safety controlling server, including being sent to above-mentioned database server
On, prevent data from taking big quantity space on calculating control server, be also prevented from calculating control server because some meanings
Lead to outward data damaged.
In addition also have some other little modules, for example, receive module of experimental considerations unit interior temperature humidity information etc., so that
These presentation of information are being calculated on the interface controlling server for laboratory director's reference.
Specific behavior electro physiology experiment is carried out in experimental considerations unit.Tasks carrying program through writing (for example, controls
Experimental facilitiess, give audiovisual stimulate, collection behavior electricity physiological signal etc.) be arranged on main control computer or working computer.Main
Experimental facilitiess real-time control module and non real-time data transmission (to c-server) module are typically contained on control computer；Work
Experimental facilitiess real-time control module and non real-time data back (to host) module are equally had on computer.Described real-time experiment
Control module is used for real-time development behavioral experiment and gathers experimental data, and described non-real-time data delivery module is used for real-time
Experimental duties gap sends the information collecting and experimental data to calculating and controls server.Main control computer and work meter
After calculation machine starts, the work such as time synchronized, equipment detection and equipment initialization should be carried out on the basis of main control computer, also will lead to
Cross s-net and calculating controls server to set up communication relations.
Above-mentioned behavior electro physiology experimental system, make use of the organizational form of solid, by multiple connected modes, including serial ports
Direct-connected and LAN is so that the accuracy of behavior electro physiology experiment, efficiency and can supervisory be greatly enhanced, especially
Be this experiment for the time exigent in the case of when Millisecond other Signal's behavior difference (such as need distinguish), this
Plant improvement to be especially apparent.
In traditional behavior electro physiology experimental system, as shown in Figure 3, only entered using a computer (whether multinuclear)
Row each task, including controlling various experimental facilitiess, gathers each class behavior electricity physiological signal and carries out signal analysis and processing.Cause
This, carry out in the experiment that electrophysiological recording carries out behavior video recording simultaneously while some give sound, color stimuluss, and some are real
Condition of testing is somewhat harsh, such as video recording size slightly larger (for example, 640 × 480), number of pictures per second slightly many (for example, more than 30 frames/second),
The real-time leading to test cannot be ensured.In addition, when computer data acquisition, if also needing to carry out data analysiss
Calculate, then for slightly more complex computing, cpu also can sink beneath one's burden.But, the behavior electro physiology being provided using the present invention
Experimental system, can control server can be counted in time after each test using calculating while data acquisition
Result after calculation, the time being spent, be exactly mainly data spread out of, result incoming and calculate control server data analysis when
Between.In a specific embodiment of the present invention, after calculating control server cpu check figure is more than 48 cores, for 20 passages
Eeg data record, above-mentioned multi-channel synchronous and correlation calculations can complete in 10 seconds, and this speed is general
More than 24 times of talking computer (double-core) processing speed.
The present invention relates to providing a kind of method carrying out behavior electro physiology experiment, the method relates to the use of multiple dimensioned nesting
, the computer network of three-dimensional, by way of LAN or serial ports are direct-connected, by experimental facilitiess, main control computer and
Calculate and couple together with controlling server system, under corresponding software system controls, constitute the behavior electro physiology experiment of cooperation
System intactly to carry out behavior electro physiology experiment, including the giving on time of stimulus signal, the Real-time Collection of experimental data, experiment
The safety storage of the on-line analyses of data and feedback and experimental data and the accurate monitoring of experiment progress.If necessary, the method
Being additionally included in behavior electro physiology experimental considerations unit assists main control computer to give to stimulate, gather using working computer further
Data and process signal, and obtained experimental data etc. is stored using database server.Using behavior electro physiology
Experimental technique, accuracy, the efficiency and can be supervisory of the experiment of behavior electro physiology can be significantly improved.
Embodiment
One macaque motor cortex electro physiology experiment, needs to record the track of macaque limb motion and corresponding cerebral cortex
EEG signals, to understand Cerebral Cortex Neuronal Cells how to control limb motion.Experimentation is as follows: animal see aobvious
Show after figure occurring on device, by the figure in regulation order Touch screen, computer is after the touch behavior judging animal is correct
Digital-to-analogue conversion plate (da plate) is controlled to open electromagnetic valve switch, to the award of a droplet water of animal.Complete whole behavior task in animal
During, cortex electric pulse data is stored in a computer after analog digital conversion plate (ad plate) reads.
According to the present invention, the experimental system of design employs three computers and is respectively completed different work: working computer a
For showing the figure of complexity, controlling feed-water valve switch, Grid Track of record animal limb motion etc.；Working computer b
For gathering the myoelectricity data of multichannel brain electric and motion limbs；Main control computer c then collect experimental data (brain electricity,
Myoelectricity and behavioral data etc.) it is sent to and calculate the result of calculation controlling server the reception server after a few seconds.Experiment sets
Standby interface and computer between is different because of concrete equipment, but main control computer needs using different network interface cards and network segment difference
Connect working computer and calculate control server.Using this experimental facilitiess connected mode, eeg signal acquisition can be made
Port number expands a lot, and experimenter can also (1～5 minute, required time be mainly to wait for animal and completes necessarily as early as possible
The behavior task of quantity, the real time calculating is within 30 seconds) know the limbs whether with animal for the EEG signals collecting
Motion is related, if related, experiment then continues, if unrelated, experimenter will brain electrode move brain its
He keeps a record position again.So can greatly accelerate the progress tested, improve conventional efficient.
In addition, experimental data is temporarily stored in calculating controlling on server, for safety, calculating and controlling server to pass through
Other network interface card is connected with database server with the network segment.As needed, animal information, experimental data will pass with testing image etc.
Deliver to and preserve for a long time on database server.
Contrast experiment: if using traditional method, on the premise of sample frequency is constant, for enabling data synchronously to adopt
Collection, the sampling channel of setting can not be too many, and the figure needing display also can not be too complicated, but also computer can not be required to count temporarily
Calculate and produce figure, can only just call in a small amount of picture previously generating before experimental duties start and wait in calculator memory makes
With.After each experimental duties terminate, carry out data analysiss calculating, because of the complicated journey of computational methods if necessary to same computer
Degree is different, needs the time (required time is mainly used in the analytical calculation of data) of 5～30 minutes under normal circumstances.If carrying out more
Complicated calculating, about needs 1 hour about sometimes.Further, since experimental data stores on the local computer, once this calculating
Machine breaks down or is attacked by computer virus, and the integrity of data will greatly be threatened.
The announcement of book and teaching according to the above description, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula is changed and is changed.Therefore, the invention is not limited in specific embodiment disclosed and described above, to the present invention's
In the scope of the claims that some modifications and changes should also be as fall into the present invention.Although additionally, using in this specification
Some specific terms and some nouns of locality, but these terms and the noun of locality are merely for convenience of description, not to the present invention
Constitute any restriction.
Claims (5)
1. a kind of behavior electro physiology experimental system, comprising:
One or more behavior electro physiology experimental considerations units, calculating control server and database server, wherein,
Described behavior electro physiology experimental considerations unit includes a main control computer and controlled experiment equipment, and described main control computer controls
Described controlled experiment equipment, to give laboratory animal stimulus signal, and Real-time Collection and the experiment number recording described laboratory animal
According to, this experimental data includes behavioural information and electro physiology experimental data,
Described behavior electro physiology experimental considerations unit also includes one or more working computer, and described main control computer passes through in unit
LAN and/or the direct-connected mode of serial ports control described working computer, control described experiment to set by described working computer
Standby to give described laboratory animal stimulus signal, the described experimental data of laboratory animal described in Real-time Collection by described experiment number
According to sending back described main control computer,
Described calculating controls server to be connected by master control LAN with described main control computer, receives described master control and calculates
The described experimental data of machine transmission is simultaneously analyzed and calculates, and
Described database server controls server to be connected by described master control LAN with described calculating, in order to store meter
Calculate data and the information that control server transmits.
2. behavior electro physiology experimental system as claimed in claim 1, wherein, described behavior electro physiology experimental considerations unit also includes net
Network CCTV camera, described network monitoring video camera passes through master control LAN and controls server to be connected with described calculating.
3. behavior electro physiology experimental system as claimed in claim 1, also includes:
Experiment control module, is arranged on described main control computer and described working computer in real time, is responsible for controlling described experiment
Equipment is carried out behavioral experiment in real time and is gathered described experimental data, and in the gap of real-time experimental duties by the described reality collecting
Test data and send described calculating control server to；
Data reception module, is arranged on described calculating and controls on server, be responsible for responding the described master control in each experimental considerations unit
The request of computer, receives the described experimental data of its transmission and this experimental data is stored in the storage calculating control server
In device, simultaneously according to its computation requests, these requests are sent to parallelization data computation analysis module, so that data is obtained in time
Calculating analysis, finally again the result calculating analysis is sent to the described main control computer of described each experimental considerations unit；
Described parallelization data computation analysis module, is arranged on described calculating and controls on server, has multiple raw with behavior electricity
Manage relevant computation module, can complete to calculate according to the corresponding assembly of the request call of described main control computer；And
Data transmitting module, is arranged on described calculating and controls on server, is responsible for needing the permanent animal information preserving, described
The video recording of experimental data and/or experiment scene is sent on described database server.
4. behavior electro physiology experimental system as claimed in claim 3, also includes:
Image receiver module, this image receiver module is arranged on described calculating and controls on server, is responsible for the described experiment of reception single
The image data of network monitoring video camera collection in unit simultaneously shows corresponding institute in real time in described calculating on control server interface
State the experiment scene in experimental considerations unit.
5. behavior electro physiology experimental system as claimed in claim 1, wherein, described calculating controls server to be multiple-core server
Or minicomputer.
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