CN104145808A - Hybrid cotton selection, production and cultivation method - Google Patents
Hybrid cotton selection, production and cultivation method Download PDFInfo
- Publication number
- CN104145808A CN104145808A CN201410371524.1A CN201410371524A CN104145808A CN 104145808 A CN104145808 A CN 104145808A CN 201410371524 A CN201410371524 A CN 201410371524A CN 104145808 A CN104145808 A CN 104145808A
- Authority
- CN
- China
- Prior art keywords
- hybrid
- male parent
- cotton
- cottons
- transformation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to the technical field of crop variety cultivation and in particular relates to a hybrid cotton selection, production and cultivation method. The hybrid cotton selection, production and cultivation method comprises the following steps: (1) selecting a new hybrid variety or an existing hybrid variety with a hybridized combination of male parent A * female parent B; (2) introducing a herbicide-resistant gene Bar to the male parent A to obtain a male parent material D; (3) producing the hybrids by taking B as the female parent and D as the male parent without castration of the female parent during the period, and adopting natural supplementary pollination by virtue of bees; and (4) planting the hybrids, killing pseudohybrids with 100 ppm of Basta in a cotton cotyledon stretching period, and adopting the rest of cultivation measures as general. Compared with sterile line hybrid cottons, the hybrid cottons obtained by the method has the advantages that the problems that selection of a restoring line is difficult, the hybrid restoring degree is instable, the combining ability between combinations is low, cottons are thinned out during the seedling period, weeding needs to be performed during the growth period and the like are solved. Compared with artificially produced hybrid cottons, the hybrid cottons obtained by the method has the advantages that the problems that the artificial production cost is high, the seed quality is difficult to guarantee, the cottons are thinned out during the seedling period, weeding needs to be performed during the growth period and the like are solved.
Description
Technical field
The present invention relates to variety of crops and cultivate technical field, is the cotton seed selection production of hybrid seeds of a kind of cotton hybrid cultivation method specifically.
Background technology
Cotton is important economic crops, have important effect, and hybrid cotton is more and more important by playing a part in following Cotton Production in the national economic development.At present, the approach that utilizes of hybrid cotton mainly contains two kinds, and the one, artificial hybridization seed production way, the 2nd, utilize the production of hybrid seeds of male sterile line mode.There is following drawback in the artificial hybridization production of hybrid seeds, and the one, amount of labour demanded is large, and during the production of hybrid seeds, mu needs 5～8 of labours; The 2nd, labour intensity is large, during the production of hybrid seeds just as heat; The 3rd, breeding cost is high, and hybrid seed per jin reaches four or five ten yuan; The 4th, production of hybrid seeds link is many, and seed quality is difficult for guaranteeing; The 5th, some Hybrids utilize the second generation of hybrid to reduce hybrid vigour.Sterile lines seed making has following drawback, and the one, the seed selection difficulty of restorer is large, and restoring degree is subject to the effect of natural conditions such as temperature; The 2nd, the coordinate force of restorer and male sterile line is low, and yield level is difficult for improving, and breeding difficulty strengthens.Cotton is dicotyledon in addition, a little less than pushing up native power, must strengthen cotton seeds consumption guarantee cotton one and broadcast full stand in cotton planting process, but strengthen the thinning problem that seed consumption must bring cotton in seedling stage, has increased labour cost.In order to accelerate the development of cotton crossbreed and the needs that cotton is simplified cultivation, we have proposed the cotton seed selection production of hybrid seeds cultivation of cotton hybrid new system.
Summary of the invention
It is difficult to the object of the invention is for three-line cotton seed selection, and the problem of the cotton production of hybrid seeds difficulty of artificial hybridization provides the cotton seed selection production of hybrid seeds of a kind of cotton hybrid cultivation method.
For achieving the above object, the technical solution used in the present invention is: the cotton seed selection production of hybrid seeds of a kind of cotton hybrid cultivation method, comprises the steps:
(1) seed selection new hybrid or existing crossbreed kind, its hybrid combination is that A male parent * B is maternal;
(2) herbicide-resistant gene Bar gene (acetyl-CoA transferase gene) is imported to male parent A, obtain male parent D material;
(3) take B carries out cross breeding seed as maternal, D as male parent, during do not carry out maternal emasculation, take the natural supplementary pollination of honeybee;
(4) plantation crossbreed, kills pseudostationary with 100ppm Basta at cotton cotyledon extensin period, and all the other cultivation steps are with conventional.
Further: in step (2), the preparation method of male parent D material is: for the male parent A material that can directly utilize agrobacterium-mediated transformation to carry out genetic transformation, utilize agrobacterium-mediated transformation that Bar gene (acetyl-CoA transferase gene) is transformed into male parent A material and obtain male parent D material.
Further: in step (2), the preparation method of male parent D material is: for can not directly utilizing agrobacterium-mediated transformation to carry out the male parent A material of genetic transformation, first Bar gene (acetyl-CoA transferase gene) is transformed in the intermediate materials C that can carry out genetic transformation, then adopts the method for backcross transformation that Bar gene (acetyl-CoA transferase gene) transformation is obtained to male parent D material in male parent A.
Useful technique effect of the present invention is: the kind that the inventive method is cultivated is compared with male sterile line hybrid cotton, has solved restorer seed selection difficult, and crossbreed restoring degree is unstable, and between combination, coordinate force is low, cotton in seedling stage thinning, the difficult problem such as weeding during cotton growth.Compare with artificial seed hybrid cotton, solved artificial seed's cost high, seed quality Customers ' Legal Right, the difficult problem such as weeding during thinning in seedling stage and cotton growth.
Embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Embodiment based in the present invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
The cotton seed selection production of hybrid seeds of a cotton hybrid cultivation method, comprises the steps:
(1) seed selection new hybrid or existing crossbreed kind, its hybrid combination is that A male parent * B is maternal;
(2) transformation crossbreed male parent A, imports male parent A herbicide-resistant gene Bar gene (acetyl-CoA transferase gene), obtains male parent D material;
The acquisition of male parent D material is to utilize agrobacterium-mediated transformation that Bar gene (acetyl-CoA transferase gene) is transformed into male parent A material to obtain male parent D material, and concrete grammar is as follows;
1. build the binary expression vector pB2GW7 that contains Bar gene, and import agrobacterium strains GV3101;
2. under aseptic condition, planting material A is on MS medium;
3. get the growth Cotton Hypocotyl of 5 days, be cut into 5～7 millimeters of segments, the Agrobacterium bacterium liquid that is 0.3～0.5 by OD value infects 10 minutes;
4. put hypocotyl segment and on inducing culture, cultivate 30 days, during add 0.05um Basta screening;
5. get 10mm left and right callus subculture and be incubated at proliferated culture medium, subculture is cultivated and to be occurred granular embryo callus 2～3 times, during add 0.05um Basta screening;
6. embryo callus is transformed on differential medium, during add 0.05um Basta screening;
7. subculture is 1～2 time, occurs regeneration seedling, during add 0.05um Basta screening;
When 8. the seedling in triangular flask grows to 8cm left and right, grafting is to solarium, during with labeled primer, screen positive strain;
9. gather in the crops T
0for seed;
10. field planting T
0for seed, selfing is isozygotied and is obtained male parent material D.
(3) take every year B carries out cross breeding seed as maternal, D as male parent, during do not carry out maternal emasculation, take the natural supplementary pollination of honeybee;
(4) plantation crossbreed, kills pseudostationary with 100ppm Basta at cotton cotyledon extensin period, and all the other cultivation steps are with conventional.
Embodiment 2
The cotton seed selection production of hybrid seeds of a cotton hybrid cultivation method, comprises the steps:
(1) seed selection new hybrid or existing crossbreed kind, its hybrid combination is that A male parent * B is maternal;
(2) transformation crossbreed male parent A, imports male parent A herbicide-resistant gene Bar gene (acetyl-CoA transferase gene), obtains male parent D material;
The preparation method of male parent D material is first Bar gene (acetyl-CoA transferase gene) to be transformed in the intermediate materials C that can carry out genetic transformation, adopt the method for backcross transformation that Bar gene (acetyl-CoA transferase gene) transformation is obtained to male parent D material in male parent A, concrete grammar is as follows again:
1. with identical transgenic method, obtain intermediate materials C;
2. by materials A, do recurrent parent, intermediate materials C does non-recurrent parent, and the transformation of Bar gene is obtained to male parent material D in materials A.
(3) take every year B carries out cross breeding seed as maternal, D as male parent, during do not carry out maternal emasculation, take the natural supplementary pollination of honeybee;
(4) plantation crossbreed, kills pseudostationary with 100ppm Basta at cotton cotyledon extensin period, and all the other cultivation steps are with conventional.
The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (3)
1. the cotton seed selection production of hybrid seeds of cotton hybrid cultivation method, is characterized in that, comprises the steps:
(1) seed selection new hybrid or existing crossbreed kind, its hybrid combination is that A male parent * B is maternal;
(2) herbicide-resistant gene Bar gene is imported to male parent A, obtain male parent D material;
(3) take every year B carries out cross breeding seed as maternal, D as male parent, during do not carry out maternal emasculation, take the natural supplementary pollination of honeybee;
(4) plantation crossbreed, kills pseudostationary with 100ppm Basta at cotton cotyledon extensin period, and all the other cultivation steps are with conventional.
2. the cotton seed selection production of hybrid seeds of cotton hybrid according to claim 1 cultivation method, it is characterized in that: in step (2), the preparation method of male parent D material is: for the male parent A material that can directly utilize agrobacterium-mediated transformation to carry out genetic transformation, utilize agrobacterium-mediated transformation that Bar genetic transformation is obtained to male parent D material to male parent A material.
3. the cotton seed selection production of hybrid seeds of cotton hybrid according to claim 1 cultivation method, it is characterized in that: in step (2), the preparation method of male parent D material is: for can not directly utilizing agrobacterium-mediated transformation to carry out the male parent A material of genetic transformation, first Bar genetic transformation to carrying out in the intermediate materials C of genetic transformation, then adopt the method for backcross transformation that the transformation of Bar gene is obtained to male parent D in male parent A.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410371524.1A CN104145808A (en) | 2014-07-30 | 2014-07-30 | Hybrid cotton selection, production and cultivation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410371524.1A CN104145808A (en) | 2014-07-30 | 2014-07-30 | Hybrid cotton selection, production and cultivation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104145808A true CN104145808A (en) | 2014-11-19 |
Family
ID=51871616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410371524.1A Pending CN104145808A (en) | 2014-07-30 | 2014-07-30 | Hybrid cotton selection, production and cultivation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104145808A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1276964A (en) * | 2000-08-09 | 2000-12-20 | 四川省农业科学院经济作物育种栽培研究所 | Production process of Za-13 as high-quality disease and pest resisting hybrid cotton |
| WO2002006500A2 (en) * | 2000-07-18 | 2002-01-24 | Pioneer Hi-Bred International, Inc. | Methods of transforming plants and identifying parental origin of a chromosome in those plants |
| CN101466837A (en) * | 2005-10-13 | 2009-06-24 | 孟山都技术有限公司 | Methods for producing hybrid seed |
| CN102119654A (en) * | 2010-11-29 | 2011-07-13 | 山东冠丰种业科技有限公司 | Method for breeding new variety of insect-resistant cotton |
-
2014
- 2014-07-30 CN CN201410371524.1A patent/CN104145808A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002006500A2 (en) * | 2000-07-18 | 2002-01-24 | Pioneer Hi-Bred International, Inc. | Methods of transforming plants and identifying parental origin of a chromosome in those plants |
| CN1276964A (en) * | 2000-08-09 | 2000-12-20 | 四川省农业科学院经济作物育种栽培研究所 | Production process of Za-13 as high-quality disease and pest resisting hybrid cotton |
| CN101466837A (en) * | 2005-10-13 | 2009-06-24 | 孟山都技术有限公司 | Methods for producing hybrid seed |
| CN102119654A (en) * | 2010-11-29 | 2011-07-13 | 山东冠丰种业科技有限公司 | Method for breeding new variety of insect-resistant cotton |
Non-Patent Citations (1)
| Title |
|---|
| 蒋玉蓉: ""棉花遗传转化方法与耐盐、抗除草剂基因的转化利用研究"", 《浙江大学学位论文》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107326042A (en) | The fixed point of paddy rice TMS10 genes knocks out system and its application | |
| CN105821073A (en) | Method of site-directed modification for intact plant by means of gene transient expression | |
| CN104521756B (en) | A kind of method producing Fructus Trichosanthis tissue cultured seedling | |
| CN102257953B (en) | Method for cultivating cucumis sativus L introgression line material for resisting meloidogyne incognita | |
| CN104221861B (en) | A kind of method utilizing embryo rescue to realize red bean and rice bean distant hybridization | |
| CN102144560B (en) | Method and application method for obtaining novel germ plasm of brassica A genome vegetable | |
| CN103583357B (en) | Method for sterile seeding of lithops and establishing regeneration system | |
| CN104823852A (en) | Dendrobium officinale rapid breeding method through root tip tissue culture | |
| CN104106470A (en) | Preparation method of porphyra yezoensis pure line germplasm protonema | |
| CN104115754B (en) | A kind of selection with the radish maintainer of Ogura male sterile cytoplasm | |
| CN107190019B (en) | A kind of sinocalamus latiflorus method for transformation of mediated by agriculture bacillus | |
| CN101810136A (en) | Cauliflower cytoplasmic male sterile line breeding method and application of male sterile line | |
| CN102498997A (en) | Method of screening nucleic sterile plants of photo-thermo-sensitive nucleic male sterile rice and method of propagating nucleic seeds | |
| CN103975855A (en) | Haploid breeding method of dendrobium candidum | |
| CN103120127A (en) | Method for quickly inducing and breeding cotton embryogenic callus | |
| CN105794509A (en) | SNP chemical regulation method for increasing thermo-sensitive genic male sterile wheat BNS maturing rate | |
| CN105145354A (en) | Method for aseptically seeding ebony and establishing leaf-cutting rapid propagation system | |
| CN105409773A (en) | Lophophora williamsii sterile seeding and regeneration system establishing method | |
| CN105104181A (en) | Haploid genome doubling method for maize | |
| CN102652477A (en) | Method for cultivating pink tomato | |
| CN104145808A (en) | Hybrid cotton selection, production and cultivation method | |
| CN104839015B (en) | Breeding method of transgenic receptor of nucleoplasmic-interactive male-sterile line of corns and application of receptor in genetic transformation and descendant propagation | |
| CN104160951B (en) | The selection of the interim maintainer completely of a kind of rapeseed plant recessive Genetic Sterility homotype | |
| CN107787827A (en) | A kind of spicy green pepper new variety selective breeding technology of precocious lower temperature resistance | |
| CN102246693B (en) | Method for creating new peanut specie in space breeding |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20141119 |