CN104045728A - Method for refining inonotus obliquus (Fr.)Pilat crude polysaccharides by adopting resin D101 - Google Patents
Method for refining inonotus obliquus (Fr.)Pilat crude polysaccharides by adopting resin D101 Download PDFInfo
- Publication number
- CN104045728A CN104045728A CN201410288076.9A CN201410288076A CN104045728A CN 104045728 A CN104045728 A CN 104045728A CN 201410288076 A CN201410288076 A CN 201410288076A CN 104045728 A CN104045728 A CN 104045728A
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- CN
- China
- Prior art keywords
- crude polysaccharides
- polysaccharides
- aqueous solution
- resin
- enzymolysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 137
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 137
- 150000004676 glycans Chemical class 0.000 title claims abstract description 135
- 238000000034 method Methods 0.000 title claims abstract description 55
- 239000011347 resin Substances 0.000 title claims abstract description 40
- 229920005989 resin Polymers 0.000 title claims abstract description 40
- 238000007670 refining Methods 0.000 title claims abstract description 15
- 241000414067 Inonotus obliquus Species 0.000 title claims abstract description 13
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 25
- 150000003384 small molecules Chemical class 0.000 claims abstract description 18
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 238000003809 water extraction Methods 0.000 claims abstract description 12
- 238000005238 degreasing Methods 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 35
- 239000000243 solution Substances 0.000 claims description 28
- 241000747105 Fuscoporia Species 0.000 claims description 24
- 239000007864 aqueous solution Substances 0.000 claims description 24
- 238000002203 pretreatment Methods 0.000 claims description 20
- 239000000049 pigment Substances 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 15
- 239000003463 adsorbent Substances 0.000 claims description 15
- 238000009835 boiling Methods 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 239000012153 distilled water Substances 0.000 claims description 11
- 238000004108 freeze drying Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 8
- 241001558929 Sclerotium <basidiomycota> Species 0.000 claims description 7
- 229920002678 cellulose Polymers 0.000 claims description 7
- 239000001913 cellulose Substances 0.000 claims description 7
- 238000004587 chromatography analysis Methods 0.000 claims description 7
- 239000000706 filtrate Substances 0.000 claims description 7
- 238000007710 freezing Methods 0.000 claims description 7
- 230000008014 freezing Effects 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 7
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 5
- 235000019441 ethanol Nutrition 0.000 claims description 5
- 230000014759 maintenance of location Effects 0.000 abstract description 4
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 238000004042 decolorization Methods 0.000 abstract description 2
- 239000012535 impurity Substances 0.000 abstract description 2
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 238000009777 vacuum freeze-drying Methods 0.000 abstract 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 11
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 241000694440 Colpidium aqueous Species 0.000 description 5
- 230000003247 decreasing effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 230000003544 deproteinization Effects 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 239000008395 clarifying agent Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000009010 Bradford assay Methods 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- -1 polysaccharide polysaccharide Chemical class 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410288076.9A CN104045728B (en) | 2014-06-25 | 2014-06-25 | Adopt the method for D101 resin for the thick polysaccharide of Inonotus obliquus |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410288076.9A CN104045728B (en) | 2014-06-25 | 2014-06-25 | Adopt the method for D101 resin for the thick polysaccharide of Inonotus obliquus |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104045728A true CN104045728A (en) | 2014-09-17 |
CN104045728B CN104045728B (en) | 2016-05-25 |
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Family Applications (1)
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CN201410288076.9A Expired - Fee Related CN104045728B (en) | 2014-06-25 | 2014-06-25 | Adopt the method for D101 resin for the thick polysaccharide of Inonotus obliquus |
Country Status (1)
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CN (1) | CN104045728B (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104293857A (en) * | 2014-09-22 | 2015-01-21 | 山东效峰生物科技股份有限公司 | Method for preparing pleurotus eryngii low molecular weight polysaccharide |
CN109134693A (en) * | 2018-11-19 | 2019-01-04 | 大兴安岭至臻尚品寒带生物技术有限公司 | A kind of preparation method of Fuscoporia obliqua polysaccharide |
CN110150668A (en) * | 2019-05-30 | 2019-08-23 | 青岛农业大学 | A kind of Inonotus obliquus enzymatic hydrolysis polyoses oral liquid |
CN114106214A (en) * | 2021-12-06 | 2022-03-01 | 山西大同大学 | Hemerocallis citrina polysaccharide and preparation method and application thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102038720A (en) * | 2010-12-17 | 2011-05-04 | 中国农业大学 | Fuscoporia obliqua active ingredients capable of lowering blood sugar and preparation method and application of fuscoporia obliqua active ingredients |
CN102579511A (en) * | 2012-03-13 | 2012-07-18 | 天津大学 | Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus |
CN102603906A (en) * | 2011-12-30 | 2012-07-25 | 安徽丰原发酵技术工程研究有限公司 | Preparation method of inonotus obliquus polysaccharide aqueous solution |
-
2014
- 2014-06-25 CN CN201410288076.9A patent/CN104045728B/en not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102038720A (en) * | 2010-12-17 | 2011-05-04 | 中国农业大学 | Fuscoporia obliqua active ingredients capable of lowering blood sugar and preparation method and application of fuscoporia obliqua active ingredients |
CN102603906A (en) * | 2011-12-30 | 2012-07-25 | 安徽丰原发酵技术工程研究有限公司 | Preparation method of inonotus obliquus polysaccharide aqueous solution |
CN102579511A (en) * | 2012-03-13 | 2012-07-18 | 天津大学 | Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus |
Non-Patent Citations (1)
Title |
---|
武永德: "两种不同来源桦褐孔菌多糖的抗氧化与免疫活性的研究", 《中国优秀硕士学位论文全文数据库(医药卫生科技辑)》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104293857A (en) * | 2014-09-22 | 2015-01-21 | 山东效峰生物科技股份有限公司 | Method for preparing pleurotus eryngii low molecular weight polysaccharide |
CN109134693A (en) * | 2018-11-19 | 2019-01-04 | 大兴安岭至臻尚品寒带生物技术有限公司 | A kind of preparation method of Fuscoporia obliqua polysaccharide |
CN110150668A (en) * | 2019-05-30 | 2019-08-23 | 青岛农业大学 | A kind of Inonotus obliquus enzymatic hydrolysis polyoses oral liquid |
CN114106214A (en) * | 2021-12-06 | 2022-03-01 | 山西大同大学 | Hemerocallis citrina polysaccharide and preparation method and application thereof |
Also Published As
Publication number | Publication date |
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CN104045728B (en) | 2016-05-25 |
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C41 | Transfer of patent application or patent right or utility model | ||
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Inventor after: Shi Lu Inventor before: Chen Wenling |
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Effective date of registration: 20160406 Address after: 100000, Beijing Haidian District information road 19, Haidian District talent exchange center on the division of collective Applicant after: Shi Lu Address before: 250014 Ji'nan Province, Lixia District, Shandong Road, No. 26, No. 1 home, home, building, culture and culture, No. 1201 Applicant before: Chen Wenling |
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Effective date of registration: 20160425 Address after: 519000 Guangdong city of Zhuhai province Hengqin Baohua Road No. 6, room 105 -12583 Applicant after: Hengqin ThinkTank Translational Medicine Co., Ltd. Address before: 100000, Beijing Haidian District information road 19, Haidian District talent exchange center on the division of collective Applicant before: Shi Lu |
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