CN104020165A - Reagent for measuring lead content of urine and rapid detection method - Google Patents
Reagent for measuring lead content of urine and rapid detection method Download PDFInfo
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- CN104020165A CN104020165A CN201410267149.6A CN201410267149A CN104020165A CN 104020165 A CN104020165 A CN 104020165A CN 201410267149 A CN201410267149 A CN 201410267149A CN 104020165 A CN104020165 A CN 104020165A
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Abstract
The invention discloses a reagent for measuring the lead content of urine and a rapid detection method, for effectively solving the problems of complexity, high cost and even incapability of testing urinary lead at times of in a conventional urinary lead detection means. According to the technical scheme, the reagent consists of 0.2 milliliter of a sodium thiocyanate solution accounting for 15 to 25 mass percent, 0.2 milliliter of a citric acid solution accounting for 0.01 to 0.05 mass percent, 0.05 milliliter of 0.025 to 0.5M strong acid and 0.05 milliliter of a crystal violet solution accounting for 0.1 to 0.5 mass percent. The reagent is convenient to use, obvious in color development, high in sensitivity, suitable for guiding various groups to detect the lead content of urine in hospitals and care service centers (agencies) of each level and extremely easy to popularize and use, can be simply and rapidly operated, and has prominent clinical application value for the prevention of urine lead poisoning, a result is accurate and intuitive, and special equipment is not required.
Description
Technical field
The present invention relates to a kind of reagent and method for quick thereof of measuring lead tolerance in urine of medical application.
Background technology
Lead is a kind of broad-spectrum heavy metal, is human body non-essential element.Along with the development of industrial and agricultural production, lead and compound thereof are day by day serious to the pollution of environment and food.Lead and compound thereof enter in body by respiratory tract, alimentary canal, skin and mucous membrane, wherein major part is stored in bone, fraction flows and is distributed to whole body each tissue and organ with blood circulation, and the sulfydryl in protein, enzyme and amino acid is combined, thereby have a strong impact on the function of nerve system of human body, hemopoietic system, digestive system and kidney.The crowd of lead contamination harm mainly contains children, pregnant woman, police, plumbous operating worker etc.In non-professional saturnism crowd, children are people at highest risk, because children are in growth and development stage, body is higher to lead poisoning neurological susceptibility; In addition, below the large multiple spurs of high concentration lead dust ground 1m, this is highly just consistent with children's breathing zone, and the lead therefore sucking is far away higher than adult, and less harm of age is larger.The feature of children's lead metabolism is that absorption is many, excretion is few.Lead is mainly to affect infant's growth and intelligent development on children's harm, and damages its cognitive function, neurobehavioral and learning and memory function.
According to detecting sample type, in body leaded quantity measuring method have send out plumbous, tooth is plumbous, bone is plumbous, tissue is plumbous, blood lead and lead in urine etc.It is larger to send out error at measurment plumbous; Tooth is plumbous, bone is plumbous and it is difficult to organize lead to draw materials; Although blood lead is drawn materials easily relatively, belongs to traumatic detection, likely infects blood borne disease; Lead in urine sampling is safe and convenient comparatively speaking.Lead enters after human body, and a part is plumbous to be accumulated; A part is excreted, and wherein 75%～80% lead is discharged through kidney, so human body is to plumbous absorption and accumulate degree, can reflect by the lead content in urine.But lead in urine detection means is loaded down with trivial details at present, cost is high, to such an extent as to sometimes cannot carry out the test of lead in urine.Therefore, how the lead content in urine is measured fast and accurately, it is very necessary being beneficial to the healthy of people.
Summary of the invention
For above-mentioned situation, for solving the defect of prior art, the present invention's object is just to provide a kind of reagent and method for quick thereof of measuring lead tolerance in urine, can effectively solve that lead in urine detection means is loaded down with trivial details, cost is high, to such an extent as to sometimes cannot carry out the problem of the test of lead in urine.
As everyone knows, detecting reagent is a kind of detection articles for use conventional in medical science, by measuring the degree of depth of coloring matter solution colour in reagent, determines component concentration to be measured.A kind of reagent and method for quick thereof of measuring lead tolerance in urine that the present invention provides according to this principle just.The crystal violet solution 0.05ml that the strong acid 0.05ml of the sodium thiocyanate solution 0.2ml that reagent is 15%～25% by massfraction, citric acid solution 0.2ml, the 0.025M～0.5M that massfraction is 0.01%～0.05% and massfraction are 0.1%～0.5% forms.Described strong acid is hydrochloric acid or sulfuric acid.
Its method for rapidly testing is in test tube, to add the tested urine of 0.5ml, then to add massfraction be the citric acid solution 0.2ml that 15%～25% sodium thiocyanate solution 0.2ml and massfraction are 0.01%～0.05%, mixes; The strong acid 0.05ml that adds again 0.025M～0.5M, after fully mixing, adding massfraction is that 0.1%～0.5% crystal violet solution 0.05ml mixes again, observations in 2 minutes.Reagent of the present invention is easy to use, and result accurately, intuitively, simple to operate, quick, and colour developing obviously, highly sensitive, without any need for special equipment.Be suitable for situation of all-level hospitals, health care institute (institute), instruct various crowds to detect urine lead tolerance, very easily penetration and promotion application, poisoning to preventing lead in urine, there is outstanding clinical use value.
Embodiment
Below in conjunction with concrete condition and embodiment, the specific embodiment of the present invention is elaborated.
By technique scheme, provided, the strong acid 0.05ml of the sodium thiocyanate solution 0.2ml that reagent of the present invention is 15%～25% by massfraction, citric acid solution 0.2ml, the 0.025M～0.5M that massfraction is 0.01%～0.05% and the crystal violet solution 0.05ml that massfraction is 0.1%～0.5% form.Strong acid can adopt hydrochloric acid or sulfuric acid; Crystal violet is a kind of commercially available dyestuff chemically.
Embodiment 1
The crystal violet solution 0.05ml that the strong acid 0.05ml of the sodium thiocyanate solution 0.2ml that reagent of the present invention is 15% by massfraction, citric acid solution 0.2ml, the 0.5M that massfraction is 0.04% and massfraction are 0.1% forms.。
Embodiment 2
The crystal violet solution 0.05ml that the strong acid 0.05ml of the sodium thiocyanate solution 0.2ml that reagent of the present invention is 20% by massfraction, citric acid solution 0.2ml, the 0.25M that massfraction is 0.03% and massfraction are 0.3% forms.
Embodiment 3
The crystal violet solution 0.05ml that the strong acid 0.05ml of the sodium thiocyanate solution 0.2ml that reagent of the present invention is 25% by massfraction, citric acid solution 0.2ml, the 0.025M that massfraction is 0.01% and massfraction are 0.5% forms.
Embodiment 4
The crystal violet solution 0.05ml that the strong acid 0.05ml of the sodium thiocyanate solution 0.2ml that reagent of the present invention is 15% by massfraction, citric acid solution 0.2ml, the 0.025M that massfraction is 0.05% and massfraction are 0.1% forms.
Embodiment 5
The crystal violet solution 0.05ml that the strong acid 0.05ml of the sodium thiocyanate solution 0.2ml that reagent of the present invention is 25% by massfraction, citric acid solution 0.2ml, the 0.5M that massfraction is 0.02% and massfraction are 0.5% forms.
For convenient, the sodium thiocyanate solution that in detection, massfraction is 15%～25% can be described as liquid No. 1, massfraction is that 0.01%～0.05% citric acid solution can be described as liquid No. 2, the strong acid of 0.025M～0.5M can be described as liquid No. 3, and the crystal violet solution that massfraction is 0.1%～0.5% can be described as liquid No. 4.
Use reagent of the present invention to detect lead tolerance method for quick in urine:
In test tube, add the tested urine of 0.5ml, then to add massfraction be the citric acid solution 0.2ml that 15%～25% sodium thiocyanate solution 0.2ml and massfraction are 0.01%～0.05%, mix; Strong acid (hydrochloric acid or the sulfuric acid all can) 0.05ml that adds again 0.025M～0.5M, after fully mixing, adding massfraction is that 0.1%～0.5% crystal violet solution 0.05ml mixes again, observations in 2 minutes was urinated lead tolerance≤9.1ug/L in 2 minutes, and reagent is purple; Urine lead tolerance is when 9.1ug/L～70ug/L, and reagent gradually becomes blue by purple; Urine lead tolerance is when 70ug/L～120ug/L, and reagent gradually becomes blue-green by blueness; During urine lead tolerance >=120ug/L, reagent is green.
The present invention is through repeatedly repeatedly on probation, all obtained satisfied effect, and compare with the dithizone colorimetric method (Pbu-SP method) of lead in urine, accuracy rate is up to 99.17%, and details are as follows to when testing the urine examination situation of 120 examples for existing and dithizone colorimetric method and the inventive method:
Method: get 16 detector tubes, in test tube, add respectively the leaded plumbous standard solution 0.5ml for 0ug/L, 5ug/L, 7ug/L, 9.1ug/L, 15ug/L, 20ug/L, 25ug/L, 30ug/L, 50ug/L, 60ug/L, 70ug/L, 80ug/L, 100ug/L, 120ug/L, 130ug/L and 150ug/L, after fully mixing, adding massfraction is the citric acid solution 0.2ml that 21% sodium thiocyanate solution 0.2ml and massfraction are 0.03% again, mixes; The strong acid 0.05ml that adds again 0.3M; After fully mixing, adding massfraction is that 0.35% crystal violet solution 0.05ml mixes again, observations in 2 minutes (result after 2 minutes is invalid).In 2 minutes, urinate lead tolerance≤9.1ug/L, reagent is purple; Urine lead tolerance is when 9.1ug/L～70ug/L, and reagent gradually becomes blue by purple; Urine lead tolerance is when 70ug/L～120ug/L, and reagent gradually becomes blue-green by blueness; During urine lead tolerance >=120ug/L, reagent is green, and the testing result of these standard solution is as the color reference of the inventive method.
The urine sample 0.5ml that gets respectively 120 examples packs in test tube, then to add massfraction be the citric acid solution 0.2ml that 21% sodium thiocyanate solution 0.2ml and massfraction are 0.03%, mixes; The strong acid 0.05ml that adds again 0.3M; After fully mixing, adding massfraction is that 0.35% crystal violet solution 0.05ml mixes again, observations in 2 minutes (result after 2 minutes is invalid).In 2 minutes, urinate lead tolerance≤9.1ug/L, reagent is purple; Urine lead tolerance, when 9.1ug/L～70ug/L, is normal value, and reagent gradually becomes blue by purple; Urine lead tolerance, when 70ug/L～120ug/L, is slight saturnism, and reagent gradually becomes blue-green by blueness; During urine lead tolerance >=120ug/L, be serious saturnism, reagent is green,
The test comparison situation of 120 examples is as following table:
By above-mentioned, can be found out, the present invention is except lead tolerance in No. 11 urine samples has abnormal (unknown origin) when 22.5ug/L in the detection of 120 examples, and other are testing requirement according to the invention all, and accuracy rate is 99.17%.Compare with additive method, as above the dithizone colorimetric method (Pbu-SP method) in table, needs spectrophotometer, complex operation step.The inventive method is easy, safe and reliable, without any need for specific apparatus.
In a word, the invention provides reagent and the rapid assay methods thereof of lead tolerance in a kind of urine that can accurately reflect plumbous metaboilic level in human body, be applicable to the external test of urine lead tolerance, with prevent with detection bodies in lead content whether exceed standard.Its result is accurate and visual, simple to operate, quick, and colour developing obviously, highly sensitive, without any need for instrument and equipment, is suitable for situation of all-level hospitals, health care institute's (institute) routine inspection and healthy primary dcreening operation etc.Its novelty of the present invention is apparent, if penetration and promotion application must be brought benefit to the mankind.
Claims (9)
1. a reagent that detects lead tolerance in urine, it is characterized in that, the strong acid 0.05ml of the sodium thiocyanate solution 0.2ml that this reagent is 15%～25% by massfraction, citric acid solution 0.2ml, the 0.025M～0.5M that massfraction is 0.01%～0.05% and the crystal violet solution 0.05ml that massfraction is 0.1%～0.5% form.
2. the reagent of lead tolerance in detection urine according to claim 1, is characterized in that, described strong acid is hydrochloric acid or sulfuric acid.
3. the reagent of lead tolerance in detection urine according to claim 1, it is characterized in that, the strong acid 0.05ml of the sodium thiocyanate solution 0.2ml that described reagent is 15% by massfraction, citric acid solution 0.2ml, the 0.5M that massfraction is 0.04% and the crystal violet solution 0.05ml that massfraction is 0.1% form.
4. the reagent of lead tolerance in detection urine according to claim 1, it is characterized in that, the strong acid 0.05ml of the sodium thiocyanate solution 0.2ml that described reagent is 20% by massfraction, citric acid solution 0.2ml, the 0.25M that massfraction is 0.03% and the crystal violet solution 0.05ml that massfraction is 0.3% form.
5. the reagent of lead tolerance in detection urine according to claim 1, it is characterized in that, the strong acid 0.05ml of the sodium thiocyanate solution 0.2ml that described reagent is 25% by massfraction, citric acid solution 0.2ml, the 0.025M that massfraction is 0.01% and the crystal violet solution 0.05ml that massfraction is 0.5% form.
6. the reagent of lead tolerance in detection urine according to claim 1, it is characterized in that, the strong acid 0.05ml of the sodium thiocyanate solution 0.2ml that described reagent is 15% by massfraction, citric acid solution 0.2ml, the 0.025M that massfraction is 0.05% and the crystal violet solution 0.05ml that massfraction is 0.1% form.
7. the reagent of lead tolerance in detection urine according to claim 1, it is characterized in that, the strong acid 0.05ml of the sodium thiocyanate solution 0.2ml that described reagent is 25% by massfraction, citric acid solution 0.2ml, the 0.5M that massfraction is 0.02% and the crystal violet solution 0.05ml that massfraction is 0.5% form.
8. adopt the method for quick of reagent described in claim 1, it is characterized in that, in test tube, add the tested urine of 0.5ml, then to add massfraction be the citric acid solution 0.2ml that 15%～25% sodium thiocyanate solution 0.2ml and massfraction are 0.01%～0.05%, mix; The strong acid 0.05ml that adds again 0.025M～0.5M, after fully mixing, adding massfraction is that 0.1%～0.5% crystal violet solution 0.05ml mixes again, and observations in 2 minutes was urinated lead tolerance≤9.1ug/L in 2 minutes, and reagent is purple; Urine lead tolerance is when 9.1ug/L～70ug/L, and reagent gradually becomes blue by purple; Urine lead tolerance is when 70ug/L～120ug/L, and reagent gradually becomes blue-green by blueness; During urine lead tolerance >=120ug/L, reagent is green.
9. the method for quick that adopts reagent described in claim 8, is characterized in that, adds the tested urine of 0.5ml in test tube, then to add massfraction be the citric acid solution 0.2ml that 21% sodium thiocyanate solution 0.2ml and massfraction are 0.03%, mixes; The strong acid 0.05ml that adds again 0.3M, after fully mixing, adding massfraction is that 0.35% crystal violet solution 0.05ml mixes again, and observations in 2 minutes was urinated lead tolerance≤9.1ug/L in 2 minutes, and reagent is purple; Urine lead tolerance is when 9.1ug/L～70ug/L, and reagent gradually becomes blue by purple; Urine lead tolerance is when 70ug/L～120ug/L, and reagent gradually becomes blue-green by blueness; During urine lead tolerance >=120ug/L, reagent is green.
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| CN201410267149.6A CN104020165B (en) | 2014-06-16 | 2014-06-16 | Reagent for measuring lead content of urine and rapid detection method |
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Cited By (1)
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| CN107328723A (en) * | 2017-05-27 | 2017-11-07 | 深圳市通量检测科技有限公司 | A kind of quick detection reagent of heavy metal lead and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107328723A (en) * | 2017-05-27 | 2017-11-07 | 深圳市通量检测科技有限公司 | A kind of quick detection reagent of heavy metal lead and preparation method thereof |
| CN107328723B (en) * | 2017-05-27 | 2019-12-06 | 深圳市通量检测科技有限公司 | rapid detection reagent for heavy metal lead and preparation method thereof |
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