CN103976725A - Awake laboratory mouse bladder pressure measuring catheter suite - Google Patents
Awake laboratory mouse bladder pressure measuring catheter suite Download PDFInfo
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- CN103976725A CN103976725A CN201410245623.5A CN201410245623A CN103976725A CN 103976725 A CN103976725 A CN 103976725A CN 201410245623 A CN201410245623 A CN 201410245623A CN 103976725 A CN103976725 A CN 103976725A
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- cystometry
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Abstract
The invention discloses an awake laboratory mouse bladder pressure measuring catheter suite which comprises a bladder cannula and a lengthening component. A guide wire is arranged inside the bladder cannula. The front end of the guide wire is designed to be a conic sharp end and the rear end of the bladder cannula is provided with a closing clamp. The lengthening component comprises a PE-50 catheter, a double-head connecting tube and a three-way catheter. One end of the PE-50 catheter is connected with the double-head connecting tube and the other end of the PE-50 catheter is connected with one catheter opening of the three-way catheter. The double-head connecting tube is connected with the rear end of the bladder cannula and fixed through the closing clamp. The awake laboratory mouse bladder pressure measuring catheter suite has the advantages that the inserting end of the bladder cannula can be fixed through an automatic curling structure generated after the guide wire of the inserting end of the bladder cannula is extracted, and the detection success rate and detection result accuracy are improved; by the use of the suite, operation difficulty is greatly lowered, experiment efficiency is improved, and damage to the skin and the bladder wall of an experiment mouse is reduced.
Description
Technical field
The present invention relates to a kind of clear-headed experimental mouse cystometry conduit suit, belong to armarium technical field.
Background technology
In Urology Surgery zoopery field, urodynamics test is the important component part of evaluation experimental animal urinary system function.Urodynamics test is according to hydrodynamics and electrophysiological ultimate principle and method, and the pressure, flow rate and the physiology electric that detect urinary tract each several part are movable, thus the function of reflection urinary tract dispatch urine and relevant dominating neural.Wherein, full property bladder pressure measure art be development ground comparatively ripe, use a kind of measuring method comparatively widely.The intrusion pipe road of the method utilization urine dynamometry system (as Powerlab or Urodynamic) to water filling in laboratory animal, utilizes its pressure measxurement pipeline to detect intravesical pressure and changes artificially.Finally, by analyzing the urine storage phase and the function of the mutual relation detection bladder of urinate phase bladder capacity and pressure variation.
Experimental rat, mice are in zoopery, to use maximum laboratory animals, and experiments of measuring Mus intravesical pressure adopts anesthesia or clear-headed experimental mouse bladder intubate manometry more both at home and abroad.The former is by experimental mouse under narcotism, and lower abdomen operates on bladder is exposed to and outside abdominal cavity, carries out intubate measurement.The method shortcoming is that bladder undergos surgery and anaesthetizes the impact of two kinds of factors, and measurement result cannot accurately reflect the bladder function of experimental mouse, even usually owing to anaesthetizing, deeply causes the detrusor of bladder cannot normal contraction.The latter both at home and abroad more adopts operation that PE-50 (polyethylene) conduit is inserted to bladder top, successively under experimental mouse abdominal cavity, rectus abdominis m. center line top, upper abdomen is subcutaneous and chest is subcutaneous, from rear cervical region, derive bladder intubate, after experimental mouse is clear-headed, a few days is carried out bladder pressure mensuration.
The method has been got rid of the impact of Anesthetic factor on bladder function, and after intubate, a few days measures bladder pressure again and can also the impact of intraoperative factors be dropped to minimum.But be faced with many serious problems:
1, PE-50 conduit easily skids off outside bladder.PE-50 conduit material is smooth, if directly insert bladder, lacks fixture, very easily skids off outside bladder.The domestic and foreign literature report fixedly PE-50 conduits of the methods such as PE-50 conduit bladder insert division purse string suture is fixed, the outer suture knotting of bladder is fixing that adopt more.These methods have not only increased fussy degree to experiment, and poor effect.Reason is that PE-50 conduit is very thin smooth difficult fixing on the one hand, and experimental mouse metabolism is rapid, movable active on the other hand, easily involves PE-50 conduit.These factors all make PE-50 conduit easily skid off outside bladder, cause and measure unsuccessfully, even cause experimental mouse dead.
2, PE-50 conduit inserts bladder difficulty.PE-50 conduit is more soft polythene material, is difficult for being directly inserted with the bladder roof construction that peritoneum covers.Domestic and foreign literature report Multi-purpose scissors is accomplished PE-50 conduit insertion end pointed, or directly with shears, on bladder top, cuts off a little otch.The former is invalid for non-full state or the thicker bladder of Musclar layer, and the trial that repeatedly interts very easily damages wall of urinary bladder, thereby affect bladder function, even causes experimental mouse death.The latter is large to wall of urinary bladder damage, faces later stage stitching simultaneously and closes many serious problems such as otch, incision leakage of urine.
3, PE-50 conduit is difficult for imbedding subcutaneous.Simple PE-50 conduit material is soft, in the situation that lacking supporting structure and guid needle, by its via experimental mouse after cervical region, chest, epigastrium and rectus abdominis m. center line top imbed subcutaneous needs and along this path, set up subcutaneous sinus tract in advance, with tweezers, insert subcutaneous gripping traction.This operation is loaded down with trivial details difficulty not only, and very big to experimental mouse wound.
Summary of the invention
Technical problem to be solved by this invention is: provide a kind of insertion end to have guiding puncture tip, side opening and carry fixed structure, integral body has the seal wire of support and indicates insertion depth referential function, extension has adjustable length can reuse the cystometry conduit suit that extends parts, bladder intubate is easily imbedded subcutaneous, easily insert bladder, before measurement, in raising stage and measuring process, position is fixed, during measurement, can get rid of the measurements interference that experimental mouse activity causes, thereby reduce the damage to experimental mouse skin and bladder, improve the success rate of intubate, the accuracy and the representativeness that reduce experimental implementation difficulty and increase testing result.
Object of the present invention is carried out specific implementation by the following technical programs:
A kind of clear-headed experimental mouse cystometry conduit suit, comprises bladder intubate and extends parts, the inside of described bladder intubate is provided with seal wire, the tip that the Front-end Design of described seal wire is taper, and the rear end of described bladder intubate is provided with sealing folder; Described prolongation parts comprise PE-50 conduit, double end connection tube and threeway conduit, and one end of described PE-50 conduit is connected with described double end connection tube, and the other end is connected with a mouth of pipe of described threeway conduit; Described double end connection tube is connected with the rear end of described bladder intubate, and clamping fixed by described sealing.
The front end of described bladder intubate is provided with indicating graduation and side opening.
Described threeway conduit is provided with pressure measurement opening and fill openings, and described fill openings is straightthrough port, and described pressure measurement opening is by-pass port.
Bladder intubate is used medical polyurethane material.
Seal wire is used stainless steel material.
PE-50 conduit uses polythene material.
Double end connection tube and threeway conduit use medical four polyvinyl fluoride materials.
The internal diameter of described bladder intubate is 0.58mm, and external diameter is 0.96mm.
The invention has the beneficial effects as follows:
1, the automatic curled structure by bladder intubate insertion end after extracting seal wire out can be fixed bladder intubate insertion end, has improved the success rate of detection and the accuracy of testing result.
2, by the puncture needle function of bladder intubate insertion end wire tips, reduced widely the difficulty of bladder intubation puncture, avoided repeated intubation or otch for the damage of experimental mouse wall of urinary bladder.
3, by the seal wire supporting structure of bladder intubate and the guid needle function of wire tips, improved the operability of bladder intubate, can be directly via subcutaneous, with tweezers, in skin fit on, along predefined paths, imbed subcutaneously, arrive punctio vesicae place.
4, bladder intubate and prolongation isolation of components, both avoided the conduit at experimental mouse back hyperpexia, can make again to extend parts and reuse, and reduces costs.Adjusting the length of prolongation parts can avoid experimental mouse activity to involve the measurements interference that pressure catheter causes simultaneously.
5, the obstruction of insertion end opening has been avoided in the side opening design of bladder intubate insertion end; Insert the design of scale sign simultaneously and can make experimenter be applicable to insertion depth according to practice summary, make experimental implementation standardization, avoid inserting shallow or excessively dark.
6, use this suit not only greatly to reduce operation easier, improve conventional efficient, and reduced the damage to experimental mouse skin and wall of urinary bladder.
Accompanying drawing explanation
According to drawings and embodiments the present invention is described in further detail below.
Fig. 1 is the structural representation of a kind of clear-headed experimental mouse cystometry conduit suit described in the embodiment of the present invention;
Fig. 2 is the local enlarged diagram in Fig. 1.
Fig. 3 is that bladder intubate is extracted the using state schematic diagram after seal wire out.
The specific embodiment
As Figure 1-3, a kind of clear-headed experimental mouse cystometry conduit suit described in the embodiment of the present invention, comprise bladder intubate 1 and extend parts, the inside of described bladder intubate 1 is provided with seal wire 2, the Front-end Design of described seal wire 2 is the tip 5 of taper, and the rear end of described bladder intubate 1 is provided with sealing folder 6; Described prolongation parts comprise PE-50 conduit 7, double end connection tube 8 and threeway conduit 9, and one end of described PE-50 conduit 7 is connected with described double end connection tube 8, and the other end is connected with a mouth of pipe of described threeway conduit 9; Described double end connection tube 8 is connected with the rear end of described bladder intubate 1, and fixing by described sealing folder 6.
The front end of described bladder intubate 1 is provided with indicating graduation 3 and side opening 4.
Described threeway conduit 9 is provided with pressure measurement opening 10 and fill openings 11, and described fill openings 11 is straightthrough port, and described pressure measurement opening 10 is by-pass port.
Bladder intubate 1 is used medical polyurethane material.
Seal wire 2 is used stainless steel material.
PE-50 conduit 7 uses polythene material.
Double end connection tube 8 and threeway conduit 9 use medical four polyvinyl fluoride materials.
The internal diameter of described bladder intubate 1 is 0.58mm, and external diameter is 0.96mm.
Experimental mouse bladder intubate step: after experimental mouse anesthesia, cervical region after sterilization, cuts off the through fascia of a little otch of 0.5cm at this place with eye scissors.At subcutaneous tip 5 of thrusting seal wire, take advantage of a situation and advance bladder intubate 1.Outside skin under the cooperation of tweezers, successively through after cervical region, chest and epigastrium that bladder intubate 1 is imbedded to experimental mouse is subcutaneous.After disinfection experiment Mus hypogastric region, get and dissect position, cut successively hypogastric region skin, fascia, rectus abdominis m., peritoneum, with tweezers gripping bladder, be exposed to outside abdominal cavity.With syringe, extract appropriate normal saline (about 5ml), by injection needle, inject bladder intubate 1 to drain wherein air.Subsequently wire tips 5 is inserted to bladder top, take advantage of a situation and advance bladder intubate 1, insertion depth is determined according to experimental mouse bladder size.Extract subsequently seal wire out, bladder intubate 1 insertion end automatic curled, is fixed on intravesical by its end.Sew up experimental mouse hypogastric region otch Musclar layer, bladder intubate 1 is derived from experimental mouse rectus abdominis m. center line top.Sew up experimental mouse hypogastric region otch skin layer, close otch.Finally after experimental mouse, cervical region bladder intubate 1 exposed junction clamps with sealing folder 6.
Experimental mouse intravesical pressure measuring process: during measurement, extract appropriate normal saline (about 20ml) with syringe, inject and extend parts to drain wherein air by injection needle.The experimental mouse of clear-headed intubate is put into metabolic cage, open sealing folder 6, connect successively double end adapter 8 and PE-50 conduit 7, PE-50 conduit 7 is pulled out outside cage and is connected with threeway conduit 9, adjust PE-50 conduit 7 length, make the activity of experimental mouse can not pull threeway conduit 9.Respectively threeway conduit pressure measurement opening 10 is connected to measure intravesical pressure with the pressure transducer of laboratory animal urine power detection system, threeway catheter perfusion opening 11 is connected with the slow water filling of constant speed with the charge pump of this system.Reaffirming bladder intubate exposed parts and extending in parts does not have bubble, starts urine dynamometry system and carries out the filling of bladder phase and the pressure detecting of the phase of urinating.After detection, pull out prolongation parts, at bladder intubate 1 exposed junction, set up sealing folder 6, finish to measure.
The invention has the beneficial effects as follows:
1, the automatic curled structure by bladder intubate insertion end after extracting seal wire out can be fixed bladder intubate insertion end, has improved the success rate of detection and the accuracy of testing result.
2, by the puncture needle function of bladder intubate insertion end wire tips, reduced widely the difficulty of bladder intubation puncture, avoided repeated intubation or otch for the damage of experimental mouse wall of urinary bladder.
3, by the seal wire supporting structure of bladder intubate and the guid needle function of wire tips, improved the operability of bladder intubate, can be directly via subcutaneous, with tweezers, in skin outside, coordinate, along predefined paths, imbed subcutaneously, arrive punctio vesicae place.
4, bladder intubate and prolongation isolation of components, both avoided the conduit at experimental mouse back hyperpexia, can make again to extend parts and reuse, and reduces costs.Adjusting the length of prolongation parts can avoid experimental mouse activity to involve the measurements interference that pressure catheter causes simultaneously.
5, the obstruction of insertion end opening has been avoided in the side opening design of bladder intubate insertion end; Insert the design of scale sign simultaneously and can make experimenter be applicable to insertion depth according to practice summary, make experimental implementation standardization, avoid inserting shallow or excessively dark.
6, use this suit not only greatly to reduce operation easier, improve conventional efficient, and reduced the damage to experimental mouse skin and wall of urinary bladder.
Claims (8)
1. a clear-headed experimental mouse cystometry conduit suit, comprises bladder intubate and extends parts, and it is characterized in that, the inside of described bladder intubate is provided with seal wire, the tip that the Front-end Design of described seal wire is taper, and the rear end of described bladder intubate is provided with sealing folder; Described prolongation parts comprise PE-50 conduit, double end connection tube and threeway conduit, and one end of described PE-50 conduit is connected with described double end connection tube, and the other end is connected with a mouth of pipe of described threeway conduit; Described double end connection tube is connected with the rear end of described bladder intubate, and clamping fixed by described sealing.
2. a kind of clear-headed experimental mouse cystometry conduit suit as claimed in claim 1, is characterized in that, the front end of described bladder intubate is provided with indicating graduation and side opening.
3. a kind of clear-headed experimental mouse cystometry conduit suit as claimed in claim 1 or 2, is characterized in that, described threeway conduit is provided with pressure measurement opening and fill openings, and described fill openings is straightthrough port, and described pressure measurement opening is by-pass port.
4. a kind of clear-headed experimental mouse cystometry conduit suit as claimed in claim 3, is characterized in that, bladder intubate is used medical polyurethane material.
5. a kind of clear-headed experimental mouse cystometry conduit suit as claimed in claim 3, is characterized in that, seal wire is used stainless steel material.
6. a kind of clear-headed experimental mouse cystometry conduit suit as claimed in claim 3, is characterized in that, PE-50 conduit uses polythene material.
7. a kind of clear-headed experimental mouse cystometry conduit suit as claimed in claim 3, is characterized in that, double end connection tube and threeway conduit use medical four polyvinyl fluoride materials.
8. a kind of clear-headed experimental mouse cystometry conduit suit as claimed in claim 3, is characterized in that, the internal diameter of described bladder intubate is 0.58mm, and external diameter is 0.96mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410245623.5A CN103976725B (en) | 2014-05-30 | 2014-05-30 | A kind of clear-headed experimental mouse cystometry conduit suit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410245623.5A CN103976725B (en) | 2014-05-30 | 2014-05-30 | A kind of clear-headed experimental mouse cystometry conduit suit |
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| Publication Number | Publication Date |
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| CN103976725A true CN103976725A (en) | 2014-08-13 |
| CN103976725B CN103976725B (en) | 2016-01-20 |
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| CN201410245623.5A Expired - Fee Related CN103976725B (en) | 2014-05-30 | 2014-05-30 | A kind of clear-headed experimental mouse cystometry conduit suit |
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Cited By (2)
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| CN104207768A (en) * | 2014-08-25 | 2014-12-17 | 翟艳萍 | Nerve disease monitor |
| CN105147305A (en) * | 2015-10-10 | 2015-12-16 | 江苏耀华医疗器械科技有限公司 | Elastic curled type urine power pipe |
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| US5427114A (en) * | 1993-08-19 | 1995-06-27 | Fiberoptic Sensor Technologies, Inc. | Dual pressure sensing catheter |
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Cited By (3)
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| CN105147305A (en) * | 2015-10-10 | 2015-12-16 | 江苏耀华医疗器械科技有限公司 | Elastic curled type urine power pipe |
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| Publication number | Publication date |
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| CN103976725B (en) | 2016-01-20 |
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