CN103869077A - Process for improving reaction activity of coating antigen - Google Patents
Process for improving reaction activity of coating antigen Download PDFInfo
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- CN103869077A CN103869077A CN201210544158.6A CN201210544158A CN103869077A CN 103869077 A CN103869077 A CN 103869077A CN 201210544158 A CN201210544158 A CN 201210544158A CN 103869077 A CN103869077 A CN 103869077A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
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Abstract
The invention provides a process for improving the reaction activity of a coating antigen. A corresponding coating buffer solution is prepared according to the characteristics of the antigen. The process is characterized in that the process comprises the following steps: 1, adding a low-concentration protein denaturant, and dissolving the protein denaturant in the prepared coating buffer solution; 2, adding a low-concentration protein reducing agent, and dissolving the protein reducing agent in the prepared coating buffer solution; 3, adding the antigen according to a determined use concentration, and stirring to make the antigen fully dissolved in the coating buffer solution containing the protein denaturant and the protein the reducing agent; 4, allowing the obtained coating buffer solution containing protein denaturant, the protein reducing agent and the antigen to stand at 2-8DEG C for 2h; and 5, carrying out antigen coating on the treated coating buffer solution obtained in step 4 according to a required coating amount. The process can effectively improve the reaction activity of the coating antigen, and improves the detection sensitivity.
Description
Technical field
The present invention relates to the preparation of in vitro diagnostic reagent, relate in particular to a kind of process that promotes envelope antigen reactivity.
Background technology
At present, immunology detection technology and products thereof is widely used in numerous detection fields such as great communicable disease, autoimmune disease, tumor markers, food security and prenatal and postnatal care, in these detection techniques as enzyme linked immunosorbent assay, the test of enzyme linked immunological spotting method, in the test of collaurum fast detecting, the coated of antigen is all requisite important step before detecting.The coated of antigen is all according to the feature of antigen self at present, as electrically charged in isoelectric point, self structure, institute etc. select corresponding coated damping fluid, coated damping fluid used comprises phosphate buffer PB, phosphate buffer PBS, carbonate buffer solution CB, Tris-Hcl damping fluid, borate buffer solution BBS etc., coated result is to make antigen be adsorbed in coated carrier surface by physical action, and follow-up immune response is carried out.The problem that existing coating technique is ignored is that antigen is adsorbed in the space structure variation producing after carrier surface, this variation can cause a part of antigenic determinant that is positioned at antigenic surface to bury in antigenic structure inside, specific antibody generation combination that cannot be corresponding with it, the antibody quantity of the specific binding producing therefrom will reduce, and finally causes detection sensitivity level to decline to some extent.Thereby the antigenic determinant that how to solve antigenic surface reduces this problem, become and promote antigenic response activity, improve detection sensitivity level and improve the key of properties of product.Therefore, require study and promote the method for antigenic response activity.
Summary of the invention
Fundamental purpose of the present invention is to overcome the above-mentioned shortcoming that existing product exists, and provide a kind of process that promotes envelope antigen reactivity, by changing composition in coated damping fluid and the processing mode of antigen, thereby promote the reactivity of envelope antigen, improve detection sensitivity.
The object of the invention is to be realized by following technical scheme.
The present invention promotes the process of envelope antigen reactivity, comprises according to the corresponding coated damping fluid of antigen own characteristic preparation; It is characterized in that, in the described coated damping fluid preparing, add successively following material: (1) adds low concentration protein denaturant, the protein denaturant adding is dissolved in the coated damping fluid preparing; (2) add low concentration protein reductive agent, the protein reductive agent adding is dissolved in the coated damping fluid preparing; (3) add antigen according to fixed working concentration, stir, it is fully dissolved in the coated damping fluid that adds protein denaturant and protein reductive agent; (4) the coated damping fluid that contains protein denaturant, protein reductive agent, antigen is placed under 2-8 ℃ of condition and is placed multiple hours; (5) by above-mentioned coated damping fluid after treatment, carry out antigen coated according to required package amount.
The process of aforesaid lifting envelope antigen reactivity, the preferred urea of protein denaturant, lauryl sodium sulfate or the sarcosyl that in the coated damping fluid wherein preparing, add, the concentration adding is 0.005 to 0.05mol/L.
The process of aforesaid lifting envelope antigen reactivity, the preferred halfcystine of protein reductive agent, tricresyl phosphate (β-chloroethyl) ester or the beta-mercaptoethanol that in the coated damping fluid wherein preparing, add, the concentration adding is 0.005 to 0.05mol/L.
The process of aforesaid lifting envelope antigen reactivity, the time that wherein the coated damping fluid that contains protein denaturant, protein reductive agent, antigen is placed in to 2-8 ℃ of condition placement is 12 to 16 hours.
The process of aforesaid lifting envelope antigen reactivity, the wherein preferred U1-nRNP of antigen, SS-B or Jo-1.
The process of aforesaid lifting envelope antigen reactivity, wherein coated damping fluid preferably phosphoric acid damping fluid (PB), phosphate buffer (PBS), carbonate buffer solution (CB), Tris-Hcl damping fluid or borate buffer solution (BBS).
The application of the process that the present invention promotes envelope antigen reactivity in enzyme linked immunosorbent assay, the test of enzyme linked immunological spotting method or the test of collaurum fast detecting.
The present invention promotes the beneficial effect of the process of envelope antigen reactivity, the present invention is coated with composition in damping fluid and the processing mode of antigen by change, the part higher structure of antigen is opened, expose more antigenic determinant, can be in conjunction with more specific antibody, thus the reactivity of antigen effectively promoted.
The present invention, by adding denaturant and the reductive agent of low concentration, reaches the reactivity that promotes envelope antigen by the processing of low temperature long period, improve the object of detection sensitivity.The denaturant of low concentration and reductive agent can partly be opened the higher structure of antigen, make up the problem that the caused antigenic surface antigenic determinant of the conformation change producing after absorption carrier reduces, avoided again simultaneously for antigen higher structure globality destroy, can not disturb the benign environment under the required low ion concns condition of immune response; The processing time that the processing mode of low temperature can make antigen keep activity stabilized, longer in processing procedure can make treatment effect reach final balance, makes differentiation drop to minimum degree.It should be noted that the impact that different denaturants and reductive agent produce antigenic structure is not quite similar, the antigenic determinant that brings changes can not perfect foresight, therefore should adopt distinctive denaturant and reductive agent for different concrete antigen.
Accompanying drawing explanation:
Fig. 1 is testing result figure after using the inventive method and using prior art processing envelope antigen.
Major label description in figure:
1-5 row are 5 parts of U1-nRNP positive sample testing results.
6-10 row are 5 parts of U1-nRNP negative sample testing results.
A is capable is to use method of the present invention to process the testing result after envelope antigen.
B is capable is the testing result using after prior art envelope antigen.
Fig. 2 is testing result figure after using the inventive method and using prior art processing envelope antigen.
Major label description in figure:
11-15 row are 5 parts of SS-B positive sample testing results.
16-20 row are 5 parts of SS-B negative sample testing results.
C is capable is to use method of the present invention to process the testing result after envelope antigen.
D is capable is the testing result using after prior art envelope antigen.
Fig. 3 is testing result figure after using the inventive method and using prior art processing envelope antigen.
Major label description in figure:
21-25 row are 5 parts of Jo-1 positive sample testing results.
26-30 row are 5 parts of Jo-1 negative sample testing results.
E is capable is to use method of the present invention to process the testing result after envelope antigen.
F is capable is the testing result using after prior art envelope antigen.
Embodiment
The present invention promotes the process of envelope antigen reactivity, comprises according to the corresponding coated damping fluid of antigen own characteristic preparation; Its improvements are to add successively following material in the described coated damping fluid preparing: (1) adds low concentration protein denaturant, and the protein denaturant adding is dissolved in the coated damping fluid preparing; (2) add low concentration protein reductive agent, the protein reductive agent adding is dissolved in the coated damping fluid preparing; (3) add antigen according to fixed working concentration, stir, it is fully dissolved in the coated damping fluid that adds protein denaturant and protein reductive agent; (4) the coated damping fluid that contains protein denaturant, protein reductive agent, antigen is placed under 2-8 ℃ of condition and is placed multiple hours; (5) by above-mentioned coated damping fluid after treatment, carry out antigen coated according to required package amount.
The present invention promotes the process of envelope antigen reactivity, wherein, the preferred urea of protein denaturant, lauryl sodium sulfate or the sarcosyl that in this coated damping fluid preparing, add, the concentration adding is 0.005 to 0.05mol/L.The preferred halfcystine of protein reductive agent, tricresyl phosphate (β-chloroethyl) ester or the beta-mercaptoethanol that in this coated damping fluid preparing, add, the concentration adding is 0.005 to 0.05mol/L.The time that the coated damping fluid that contains protein denaturant, protein reductive agent, antigen is placed in to 2-8 ℃ of condition placement is 12 to 16 hours.The preferred U1-nRNP of this antigen, SS-B or Jo-1.This coated damping fluid preferably phosphoric acid damping fluid (PB), phosphate buffer (PBS), carbonate buffer solution (CB), Tris-Hcl damping fluid or borate buffer solution (BBS).
The present invention promotes the process of envelope antigen reactivity and can in enzyme linked immunosorbent assay, the test of enzyme linked immunological spotting method or the test of collaurum fast detecting, apply.
Embodiment 1:
In 1000ml distilled water, add successively following material:
A prepares 0.02 mol/L phosphate buffer PBS, pH7.2;
B adds urea in above-mentioned damping fluid, and concentration is 0.005mol/L, stirs, and it is fully dissolved;
C adds analytically pure halfcystine, and concentration is 0.005mol/L, stirs, and it is fully dissolved;
D adds U1-nRNP antigen, and concentration 1% stirs, and it is fully dissolved;
E is placed in 2-8 ℃ of condition by the above-mentioned buffer solution that contains urea, halfcystine, U1-nRNP antigen and places processing 12 hours;
F uses above-mentioned through 2-8 ℃ of condition solution after treatment, is coated with according to enzyme linked immunological spotting method according to the package amount of 1 μ l/cm.
As shown in Figure 1,1-5 row are 5 parts of U1-nRNP positive sample testing results, and 6-10 row are 5 parts of U1-nRNP negative sample testing results.A is capable is to use method of the present invention to process the testing result after envelope antigen, and B is capable is the testing result using after prior art envelope antigen.Without colour developing band, in the middle of positive sample patch, colour developing band is darker, illustrate that the positive degree of sample is stronger in the middle of negative sample patch, and for a positive sample, the band that develops the color illustrates that envelope antigen reactivity is stronger more deeply, and detection sensitivity level is higher.Be can clearly be seen that by accompanying drawing, in 5 parts of U1-nRNP positive sample, processed the testing result colour developing band of envelope antigen through the inventive method and obviously deepen than the testing result colour developing band of prior art, and two kinds of methods of 5 parts of U1-nRNP negative sample testing results are consistent.Presentation of results, disposal route of the present invention can effectively promote the reactivity of envelope antigen, improves detection sensitivity level.
Embodiment 2:
In 1000ml distilled water, add successively following material:
A prepares 0.02 mol/L carbonate buffer solution (CB), pH9.6;
B adds lauryl sodium sulfate in above-mentioned damping fluid, and concentration is 0.02mol/L, stirs, and it is fully dissolved;
C adds analytically pure tricresyl phosphate (β-chloroethyl) ester, and concentration is 0.02mol/L, stirs, and it is fully dissolved;
D adds SS-B antigen, and concentration 2% stirs, and it is fully dissolved;
E is placed in 2-8 ℃ of condition by the above-mentioned buffer solution that contains lauryl sodium sulfate, tricresyl phosphate (β-chloroethyl) ester, SS-B antigen and places processing 14 hours;
F uses above-mentioned through 2-8 ℃ of condition solution after treatment, is coated with according to enzyme linked immunological spotting method according to the package amount of 1 μ l/cm.
As shown in Figure 2,11-15 row are 5 parts of SS-B positive sample testing results, and 16-20 row are 5 parts of SS-B negative sample testing results.C is capable is to use method of the present invention to process the testing result after envelope antigen, and D is capable is the testing result using after prior art envelope antigen.Without colour developing band, in the middle of positive sample patch, colour developing band is darker, illustrate that the positive degree of sample is stronger in the middle of negative sample patch, and for a positive sample, the band that develops the color illustrates that envelope antigen reactivity is stronger more deeply, and detection sensitivity level is higher.Be can clearly be seen that by accompanying drawing, in 5 parts of SS-B positive sample, processed the testing result colour developing band of envelope antigen through the inventive method and obviously deepen than the testing result colour developing band of prior art, and two kinds of methods of 5 parts of SS-B negative sample testing results are consistent.Presentation of results, disposal route of the present invention can effectively promote the reactivity of envelope antigen, improves detection sensitivity level.
Embodiment 3:
In 1000ml distilled water, add successively following material:
A prepares 0.05 mol/L Tris-Hcl damping fluid, pH7.2;
B adds sarcosyl in above-mentioned damping fluid, and concentration is 0.05mol/L, stirs, and it is fully dissolved;
C adds beta-mercaptoethanol, and concentration is 0.05mol/L, stirs, and it is fully dissolved;
D adds Jo-1 antigen, and concentration 1% stirs, and it is fully dissolved;
E is placed in 2-8 ℃ of condition by the above-mentioned buffer solution that contains sarcosyl, beta-mercaptoethanol, Jo-1 antigen and places processing 16 hours;
F uses above-mentioned through 2-8 ℃ of condition solution after treatment, is coated with according to enzyme linked immunological spotting method according to the package amount of 1 μ l/cm.
As shown in Figure 3,21-25 row are 5 parts of Jo-1 positive sample testing results, and 26-30 row are 5 parts of Jo-1 negative sample testing results.E is capable is to use method of the present invention to process the testing result after envelope antigen, and F is capable is the testing result using after prior art envelope antigen.Without colour developing band, in the middle of positive sample patch, colour developing band is darker, illustrate that the positive degree of sample is stronger in the middle of negative sample patch, and for a positive sample, the band that develops the color illustrates that envelope antigen reactivity is stronger more deeply, and detection sensitivity level is higher.Be can clearly be seen that by accompanying drawing, in 5 parts of Jo-1 positive sample, processed the testing result colour developing band of envelope antigen through the inventive method and obviously deepen than the testing result colour developing band of prior art, and two kinds of methods of 5 parts of Jo-1 negative sample testing results are consistent.Presentation of results, disposal route of the present invention can effectively promote the reactivity of envelope antigen, improves detection sensitivity level.
Use through 2-8 ℃ of condition buffer solution after treatment, can also be coated with according to collaurum fast detecting test method according to the package amount of 1 μ l/cm, can also be coated with according to enzyme-linked immunologic adsorption test method according to the package amount in 100 μ l/ holes.
In the present embodiment, be not prior art by the content describing, therefore, no longer repeat.
The above, it is only preferred embodiment of the present invention, not the present invention is done to any pro forma restriction, any simple modification, equivalent variations and modification that every foundation technical spirit of the present invention is done above embodiment, all still belong in the scope of technical solution of the present invention.
Claims (7)
1. promote a process for envelope antigen reactivity, comprise according to the corresponding coated damping fluid of antigen own characteristic preparation; It is characterized in that, in the described coated damping fluid preparing, add successively following material: (1) adds low concentration protein denaturant, the protein denaturant adding is dissolved in the coated damping fluid preparing; (2) add low concentration protein reductive agent, the protein reductive agent adding is dissolved in the coated damping fluid preparing; (3) add antigen according to fixed working concentration, stir, it is fully dissolved in the coated damping fluid that adds protein denaturant and protein reductive agent; (4) the coated damping fluid that contains protein denaturant, protein reductive agent, antigen is placed under 2-8 ℃ of condition and is placed multiple hours; (5) by above-mentioned coated damping fluid after treatment, carry out antigen coated according to required package amount.
2. the process of lifting envelope antigen reactivity according to claim 1, it is characterized in that, the preferred urea of protein denaturant, lauryl sodium sulfate or the sarcosyl that in the described coated damping fluid preparing, add, the concentration adding is 0.005 to 0.05mol/L.
3. the process of lifting envelope antigen reactivity according to claim 1, it is characterized in that, the preferred halfcystine of protein reductive agent, tricresyl phosphate (β-chloroethyl) ester or the beta-mercaptoethanol that in the described coated damping fluid preparing, add, the concentration adding is 0.005 to 0.05mol/L.
4. the process of lifting envelope antigen reactivity according to claim 1, is characterized in that, the time that the described coated damping fluid that contains protein denaturant, protein reductive agent, antigen is placed in to 2-8 ℃ of condition placement is 12 to 16 hours.
5. the process of lifting envelope antigen reactivity according to claim 1, is characterized in that: the preferred U1-nRNP of described antigen, SS-B or Jo-1.
6. the process of lifting envelope antigen reactivity according to claim 1, is characterized in that: described coated damping fluid preferably phosphoric acid damping fluid (PB), phosphate buffer (PBS), carbonate buffer solution (CB), Tris-Hcl damping fluid or borate buffer solution (BBS).
7. the application of the process of a lifting envelope antigen reactivity as claimed in claim 1 in enzyme linked immunosorbent assay, the test of enzyme linked immunological spotting method or the test of collaurum fast detecting.
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| CN201210544158.6A CN103869077A (en) | 2012-12-14 | 2012-12-14 | Process for improving reaction activity of coating antigen |
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| CN201210544158.6A CN103869077A (en) | 2012-12-14 | 2012-12-14 | Process for improving reaction activity of coating antigen |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106153905A (en) * | 2016-06-21 | 2016-11-23 | 广西中医药大学附属瑞康医院 | A kind of test kit detecting HIV (human immunodeficiency virus) |
| CN107271657A (en) * | 2016-04-08 | 2017-10-20 | 北京爱普拜生物技术有限公司 | A kind of protein immunoblotting signal enhancing agent |
| CN107727856A (en) * | 2017-09-30 | 2018-02-23 | 广州万孚生物技术股份有限公司 | Detect the test strips of HIV antibody, detection line coating buffer and preparation method thereof in urine |
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| CN1294301A (en) * | 1999-11-01 | 2001-05-09 | 扬州大学 | Enzymoimmune reagent kit |
| US20040152070A1 (en) * | 2003-02-04 | 2004-08-05 | Shah Dinesh O. | Method of detection of HCV antibodies in combination assay or sole antibody assay |
| WO2006081718A1 (en) * | 2005-02-02 | 2006-08-10 | Peng Cui | Kit and method for the detection of anti-hepatitis c virus (hcv) antibodies |
| CN101968490A (en) * | 2010-08-26 | 2011-02-09 | 广东省农业科学院兽医研究所 | Indirect ELISA (Enzyme-Linked Immunosorbent Assay) method and kit for detecting haemophilus parasuis antibodies |
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2012
- 2012-12-14 CN CN201210544158.6A patent/CN103869077A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1294301A (en) * | 1999-11-01 | 2001-05-09 | 扬州大学 | Enzymoimmune reagent kit |
| US20040152070A1 (en) * | 2003-02-04 | 2004-08-05 | Shah Dinesh O. | Method of detection of HCV antibodies in combination assay or sole antibody assay |
| WO2006081718A1 (en) * | 2005-02-02 | 2006-08-10 | Peng Cui | Kit and method for the detection of anti-hepatitis c virus (hcv) antibodies |
| CN101968490A (en) * | 2010-08-26 | 2011-02-09 | 广东省农业科学院兽医研究所 | Indirect ELISA (Enzyme-Linked Immunosorbent Assay) method and kit for detecting haemophilus parasuis antibodies |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107271657A (en) * | 2016-04-08 | 2017-10-20 | 北京爱普拜生物技术有限公司 | A kind of protein immunoblotting signal enhancing agent |
| CN107271657B (en) * | 2016-04-08 | 2018-06-05 | 北京爱普拜生物技术有限公司 | A kind of protein immunoblotting signal enhancing agent |
| CN106153905A (en) * | 2016-06-21 | 2016-11-23 | 广西中医药大学附属瑞康医院 | A kind of test kit detecting HIV (human immunodeficiency virus) |
| CN107727856A (en) * | 2017-09-30 | 2018-02-23 | 广州万孚生物技术股份有限公司 | Detect the test strips of HIV antibody, detection line coating buffer and preparation method thereof in urine |
| CN107727856B (en) * | 2017-09-30 | 2020-11-06 | 广州万孚生物技术股份有限公司 | Test strip for detecting HIV antibody in urine, detection line coating solution and preparation method thereof |
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Application publication date: 20140618 |