CN103805524A - Glomus versiforme LCGX-58 and applications thereof - Google Patents
Glomus versiforme LCGX-58 and applications thereof Download PDFInfo
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- CN103805524A CN103805524A CN201410078286.5A CN201410078286A CN103805524A CN 103805524 A CN103805524 A CN 103805524A CN 201410078286 A CN201410078286 A CN 201410078286A CN 103805524 A CN103805524 A CN 103805524A
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Abstract
The invention relates to Glomus versiforme LCGX-58 which is collected in the CGMCC (China General Microbiological Culture Collection Center), with the collection number of CGMCC No:8775. Tested by potting and field experiments, the strain plays a role in promoting the growth of sugarcane, is strong in adaptability and colonization power under natural field conditions, high in infection rate of sugarcane roots, and capable of promoting the absorption of sugarcane roots on nutrition in soil and reducing the application amount of fertilizer, thereby being a microbial agent applicable to production of sugarcanes.
Description
Technical field
The present invention relates to microbiobacterial agent field, specifically strain earth's surface sacculus mould (Glomus versiforme) LCGX-58 and the application in the sugarcane production of land for growing field crops thereof.
Background technology
Arbuscular mycorrhiza (Arbuscular Mycorrhizal, AM) be the reciprocal symbiosis body being formed by mould (Glomeromycota) fungi of sacculus and root system of plant, approximately more than 80% land plant can form arbuscular mycorrhiza, is the most general a kind of mycorrhizal type of occurring in nature.After AM fungi and root system of plant symbiosis, can promote absorption and the utilization of host plant to moisture and Soil Mineral Elements, particularly phosphorus, improve the utilization ratio of nutrient, Promoting plant growth, improves crop yield and quality.
AM fungi plays a significant role improving in crop rhizosphere soils environment.AM fungi, in the huge mycelia network system of plant rhizosphere formation, can increase the root system surface-area of host plant, improves growing environment and the receptivity of root system, improves the resistance of crop.Its nutritive substance and moisture in can fixing soil, the crumb structure of stable soil, improves the materialization property of continuous cropping soil.AM fungus secretion soil enzyme, as Phosphoric acid esterase, urase etc., and improves its activity, indirectly nutritive element, pH and the flora structure of balance continuous cropping soil.Promote to produce because of continuous cropping degraded and the conversion of the organic contaminants in soil that causes enrichment.AM fungi all has restraining effect to the common soil-borne disease insect pest of most of continuous croppings, can significantly suppress the breeding of pathogenic bacteria and infect, and effectively reduces the harm of continuous cropping disease and pest.
Therefore, the inhibition to soil-borne disease in stable soil structure, the conservation of soil and water, enhancing of AM microbial inoculum, improve the aspect such as nutrient composition and the biological community structure performance keying action of Rhizosphere ecosystem position, can reach and make crop yield, improve the object of its economic benefit.Especially the soil remediation function of AM fungi plays a significant role in the ecological agriculture.
Summary of the invention
The object of this invention is to provide strain earth's surface sacculus mould LCGX-58 and the application in the sugarcane production of land for growing field crops thereof.
A strain earth's surface sacculus mould LCGX-58 provided by the invention, is isolated from perennial root Among the Sugarcane root soil for many years, and earth's surface sacculus mould LCGX-58 is a type in bush mycorrhizal fungi Paraglomus.Sugarcane production is had to the earth's surface sacculus mould of obvious promoter action, this bacterial strain was deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 01 16th, 2014, preserving number is CGMCC No:8775, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
The morphological specificity of earth's surface sacculus mould LCGX-58: spore, Dan Sheng in soil, sporocarp the unknown, spore is spherical or subsphaeroidal, yellow to yellowish brown, has a circle dark color, big or small 82-136 μ m under microscope along sporoderm.3 layers of sporoderms, the L1 water white transparency wall that easily dies, approximately 1 μ m, maturation comes off or surplus part detritus only often, and L2 is pale yellow to yellowish brown stratiform wall, thick 2-4 μ m, thickness is more even.The single wall of L3, approximately 1 μ m, is difficult for separating with L2 burnished gold.Connect spore mycelia: water white transparency, wide 4.3-6.8 μ m, normal atrophy or come off in ripe spore.Connect the wide 7.6-12.0 μ m of point, straight or litmus shape, open or formed by an inwall every sealing.Inclusion oil droplet shape.
The present invention also provides the microbiobacterial agent of being prepared by earth's surface sacculus mould LCGX-58.Take corn as host, by spore, the mycelia of earth's surface sacculus mould LCGX-58 with comprise this bacterium host plant root system and culture medium is inoculated in maize seedling roots, plant in carrying out in the culture medium of 121 ℃ of high pressure steam sterilization 1h with river sand: zeolite=3:1 potted plant, illumination cultivation 12-16 week.The about 100mL of every 7-10d cast 25-50%Hoagland nutritive medium.Using the mycelia, spore and the culture medium that are obtained as LCGX-58 microbial inoculum.
The present invention also provides the application in the sugarcane production of land for growing field crops by the prepared microbial inoculum of earth's surface sacculus mould LCGX-58.
Land for growing field crops sugarcane inoculation test: start ditch according to sugarcane general planting, earth's surface sacculus mould LCGX-58 microbial inoculum is imposed on to row ditch bottom, each simple bud or two bud joint sugarcane kind stem are executed this microbial inoculum 10-20g, its spore amount be 60-100 spore/gram.
The outstanding beneficial effect of the present invention is:
Isolated earth's surface sacculus mould LCGX-58 from perennial root Among the Sugarcane root soil for many years, has good promoter action by potted plant and field experiment checking bacterial strain LCGX-58 to sugarcane production.Under the natural condition of field, adaptive faculty and colonazition are strong, can promote the absorption of sugarcane to nutrient in soil, particularly phosphorus, reduce the amount of application of chemical fertilizer, improve ecological environment of soil, are the desirable microbiobacterial agents that is suitable for land for growing field crops sugarcane production.
Accompanying drawing explanation
Fig. 1 is the form collection of illustrative plates of earth's surface sacculus mould LCGX-58.
Fig. 2 is the pot experiment of earth's surface sacculus mould LCGX-58 microbial inoculum.
Embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.If do not specialize, the conventional means that in embodiment, technique means used is well known to those skilled in the art, the raw materials used commercial goods that is.
Embodiment 1
The preparation of earth's surface sacculus mould LCGX-58 microbial inoculum
Take corn as host, by the spore inoculating of earth's surface sacculus mould LCGX-58 in maize seedling roots, plant in carrying out in the culture medium of 121 ℃ of high pressure steam sterilization 1h with river sand: zeolite=3:1 potted plant, illumination cultivation 12-16 week.The about 100mL of every 7-10d cast 25-50%Hoagland nutritive medium.Using the mycelia, spore and the matrix that are obtained as LCGX-58 microbial inoculum.
Embodiment 2
The sugarcane pot experiment of earth's surface sacculus mould LCGX-58 microbial inoculum
River sand at basin alms bowl intermediate-frequency strip through 121 ℃ of high pressure steam sterilization 1h: the culture medium of zeolite=3:1, add earth's surface sacculus mould LCGX-58 microbial inoculum 10-20 gram, cover aseptic culture medium 2-3cm, put simple bud sugarcane kind stem 2-3, cover aseptic substrate.Contrast is not to add earth's surface sacculus mould LCGX-58 microbial inoculum, and other plantation is identical.The about 100mL of every 7-10d cast 25-50%Hoagland nutritive medium.6 months " Invest, Then Investigate " plant strain growth situations.
Test-results shows, the sugarcane plant root of inoculation earth's surface sacculus mould LCGX-58 microbial inoculum is more flourishing, and plant height is higher than contrast, and greenery number and stem footpath all have superiority than contrast, see shown in accompanying drawing 2.
Embodiment 3
The land for growing field crops sugarcane test of earth's surface sacculus mould LCGX-58 microbial inoculum
(1) test design
Test is divided into 4 processing:
(A) LCGX-58 microbial inoculum+composite fertilizer;
(B) composite fertilizer;
(C) LCGX-58 microbial inoculum;
(D) blank.
3 communities of each processing, 5.5 meters of 7 meters of X of each community area.Wherein 1.1 meters of line-spacings, totally 5 row.7 meters of every row, 50 two bud joints of the amount of sowing, totally 100 buds.The N:P:K=15:5:15 of composite fertilizer used, is the La Duomei composite fertilizer that Guangdong Laduomei Chemical Fertilizer Co., Ltd. produces, and amount of application is 50kg/ mu.
Start ditch according to sugarcane general planting, earth's surface sacculus mould LCGX-58 microbial inoculum is imposed on to row ditch bottom, each simple bud or two bud joint sugarcane kind stem are executed this microbial inoculum 10-20g, its spore amount be 60-100 spore/gram.
(2) test-results
Land for growing field crops sugarcane test-results shows, executes earth's surface sacculus mould LCGX-58 microbial inoculum and can improve the content of available N and P and potassium in soil; Improve the pH value of soil, promote the absorption of root system to rhizosphere soil trace element, be shown in Table 1.Increase plant height, number of productive tiller and stem footpath, effectively promote sugarcane strain growth.Use earth's surface sacculus mould LCGX-58 microbial inoculum, compared with blank, can improve number of productive tiller 16.9%, output improves 9.97%; LCGX-58 microbial inoculum+composite fertilizer improves 12.88% than the output of composite fertilizer, is shown in Table 2.
Table 1 is used sacculus mould LCGX-58 microbial inoculum front and back, earth's surface soil physico-chemical property changing conditions
Remarks: A-LCGX-58 microbial inoculum+composite fertilizer; B-composite fertilizer; C-LCGX-58 microbial inoculum; D-blank.
Inoculation earth's surface, table 2 sugarcane land for growing field crops sacculus mould LCGX-58 microbial inoculum effect analysis
Remarks: A-LCGX-58 microbial inoculum+composite fertilizer; B-composite fertilizer; C-LCGX-58 microbial inoculum; D-blank.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (5)
1. strain earth's surface sacculus mould (Glomus versiforme) LCGX-58, is characterized in that, this bacterial strain was deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 01 16th, 2014, and preserving number is CGMCC No:8775.
2. the cultural method of earth's surface claimed in claim 1 sacculus mould (Glomus versiforme) LCGX-58, it is characterized in that, take corn as host, by spore, the mycelia of earth's surface sacculus mould (Glomus versiforme) LCGX-58 with comprise this bacterial strain host plant root system and culture medium is inoculated in maize seedling roots, plant in carrying out in the culture medium of 121 ℃ of high pressure steam sterilization 1h with river sand: zeolite=3:1 potted plant, illumination cultivation 12-16 week.
3. the microbiobacterial agent that prepared by earth's surface claimed in claim 1 sacculus mould (Glomus versiforme) LCGX-58.
4. earth's surface claimed in claim 1 sacculus mould (Glomus versiforme) LCGX-58 or the application of microbiobacterial agent claimed in claim 3 in sugarcane production.
5. earth's surface according to claim 4 sacculus mould (Glomus versiforme) LCGX-58 or the application of microbiobacterial agent in sugarcane production, it is characterized in that, before cane planting, earth's surface sacculus mould (Glomus versiforme) LCGX-58 microbial inoculum is imposed on to row ditch bottom, each simple bud or two bud joint sugarcane kind stem is executed this microbial inoculum 10-20g, its spore amount be 60-100 spore/gram.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861316A (en) * | 2016-04-05 | 2016-08-17 | 中国科学院生态环境研究中心 | Abuscular mycorrhizal fungus and application thereof |
| CN107018883A (en) * | 2017-01-06 | 2017-08-08 | 广西壮族自治区农业科学院微生物研究所 | A kind of method for producing banana mycorhiza tissue culture of sprout |
| CN109874808A (en) * | 2019-01-25 | 2019-06-14 | 青岛农业大学 | The application of arbuscular mycorrhizal fungi and/or plant symbiosis actinomyces in the biological prevention and control agent that preparation is used for capsicum and eggplant |
| CN111034741A (en) * | 2019-12-17 | 2020-04-21 | 华南农业大学 | Application of arbuscular mycorrhizal fungi in promoting pesticide absorption of crops |
| CN113388528A (en) * | 2021-06-15 | 2021-09-14 | 中国林业科学研究院林业研究所 | Ground surface sacculus mould, composition for saline-alkali soil remediation and saline-alkali soil remediation method |
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| DE102009001728A1 (en) * | 2009-03-23 | 2010-09-30 | Bayer Cropscience Ag | Improving the production potential of transgenic plant, by combating e.g. animal pests and/or microorganism, and/or increasing plant health, comprises treating the plants with active agent composition comprising fluoxastrobin |
| CN102986447A (en) * | 2013-01-07 | 2013-03-27 | 广西壮族自治区农业科学院微生物研究所 | Method for promoting growth of Datian sugarcanes by using arbuscular mycorrhizal fungal inoculant |
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2014
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Patent Citations (2)
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| DE102009001728A1 (en) * | 2009-03-23 | 2010-09-30 | Bayer Cropscience Ag | Improving the production potential of transgenic plant, by combating e.g. animal pests and/or microorganism, and/or increasing plant health, comprises treating the plants with active agent composition comprising fluoxastrobin |
| CN102986447A (en) * | 2013-01-07 | 2013-03-27 | 广西壮族自治区农业科学院微生物研究所 | Method for promoting growth of Datian sugarcanes by using arbuscular mycorrhizal fungal inoculant |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861316A (en) * | 2016-04-05 | 2016-08-17 | 中国科学院生态环境研究中心 | Abuscular mycorrhizal fungus and application thereof |
| CN105861316B (en) * | 2016-04-05 | 2019-05-07 | 中国科学院生态环境研究中心 | One plant of arbuscular mycorrhizal fungi and its application |
| CN107018883A (en) * | 2017-01-06 | 2017-08-08 | 广西壮族自治区农业科学院微生物研究所 | A kind of method for producing banana mycorhiza tissue culture of sprout |
| CN109874808A (en) * | 2019-01-25 | 2019-06-14 | 青岛农业大学 | The application of arbuscular mycorrhizal fungi and/or plant symbiosis actinomyces in the biological prevention and control agent that preparation is used for capsicum and eggplant |
| CN109874808B (en) * | 2019-01-25 | 2021-02-02 | 青岛农业大学 | Application of arbuscular mycorrhizal fungi and/or plant symbiotic actinomycetes in preparation of biocontrol preparation for hot pepper and solanaceae vegetables |
| CN111034741A (en) * | 2019-12-17 | 2020-04-21 | 华南农业大学 | Application of arbuscular mycorrhizal fungi in promoting pesticide absorption of crops |
| CN113388528A (en) * | 2021-06-15 | 2021-09-14 | 中国林业科学研究院林业研究所 | Ground surface sacculus mould, composition for saline-alkali soil remediation and saline-alkali soil remediation method |
| CN113388528B (en) * | 2021-06-15 | 2022-07-08 | 中国林业科学研究院林业研究所 | Ground surface sacculus mould, composition for saline-alkali soil remediation and saline-alkali soil remediation method |
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Application publication date: 20140521 |