CN103773679A - Automatic bacterial colony counter - Google Patents
Automatic bacterial colony counter Download PDFInfo
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- CN103773679A CN103773679A CN201210399091.1A CN201210399091A CN103773679A CN 103773679 A CN103773679 A CN 103773679A CN 201210399091 A CN201210399091 A CN 201210399091A CN 103773679 A CN103773679 A CN 103773679A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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Abstract
The invention relates to an automatic bacterial colony counter. The automatic bacterial colony counter comprises a shell portion, a circuit portion, an image acquisition portion and a detection bin automatic door portion, the image acquisition portion comprises an image acquisition high definition CCD camera part having tens of millions of pixels, and a light source system part, and the circuit portion and the detection bin automatic door portion are respectively installed on the baseboard of the shell portion. The automatic bacterial colony counter has the characteristics of good image acquisition quality, high automation degree and polychromatic light background.
Description
Technical field
What the present invention relates to is a kind of detecting instrument, is specifically related to a kind of instrument that bacterium colony is counted.
Background technology
The mensuration of total number of bacterial colony is the degree for judging that atmosphere, water quality and food are contaminated by bacterial, and it reflects whether atmosphere, water quality and food meet the mankind's service requirements, to detected sample is made to suitable hygienical evaluation.The number of total number of bacterial colony indicates the quality of the situations such as atmosphere, water quality and food to a certain extent.Bacterial colony counting instrument is counted the bacterium colony sample of cultivating out, makes a judgement accurately for the degree that atmosphere, water quality and food are contaminated by bacterial.
Traditional enumeration instrument adopts detection storehouse not seal or detect the mode of the artificial folding in storehouse mostly in the market, imaging source uses monochromatic ray, these sampling instruments in use there will be some problems so at present: do not seal if detect storehouse, in the process gathering at image, outdoor optical, according to the acquisition quality that has a strong impact on image, is unfavorable for the counting of bacterium colony; Manually, to detecting storehouse folding, complex operation, greatly reduces working efficiency; Monochromatic source can affect the bacterium colony background collection effect of different colours.
Summary of the invention
The invention provides a kind of Automatic Colony Counter, can solve the weak point that current technology exists, the Automatic Colony Counter that a kind of IMAQ quality is good, level of automation is high, polychromatic light shines background is provided.
For achieving the above object, technical scheme of the present invention is:
A kind of Automatic Colony Counter, comprises housing parts, circuit part, IMAQ part and detects storehouse automatically-controlled door part, circuit part comprises touch display screen and master control board.IMAQ part comprises IMAQ millions pixel high definition CCD(image sensor) camera part and light-source system part, described circuit part, detection storehouse automatically-controlled door part are installed on the base plate of described housing parts; Wherein IMAQ millions pixel high definition CCD camera section is divided and is comprised CCD camera, Camera fixing seat, camera mounting flange.CCD camera is arranged on Camera fixing seat, and Camera fixing seat connects with camera mounting flange, and camera mounting flange is connected on shell upper boss.CCD camera by connection to master control board, master control board by connection to touch display screen.Can regulate the flexible of CCD camera lens by the operation of touch display screen, thereby complete the adjusting to CCD camera lens magnification.
Described light-source system part comprises all-colour LED (photodiode) lamp, LED lamp circuit board, light guiding plate, diffuser plate, clamping sleeve and frosted plate.Full-color LED lamp is uniform to be arranged on LED lamp circuit board, and light guiding plate and diffuser plate are arranged on LED lamp circuit board, and frosted plate is arranged on rotating base, and clamping sleeve is arranged on rotating base and compresses frosted plate.Full-color LED lamp is distributed on light guiding plate surrounding, LED lamp circuit board is connected with master control board by circuit, form a circular luminous array, by touch display screen, backlight color data are set, to be transferred to master control board by circuit, by the proportion of the three kinds of primary colors of all-colour LED gray scale setting module regulating and controlling red, green, blue on master control board, thereby blend different backlight colors, bias light is spread in the detection storehouse of sealing uniformly by light guiding plate and diffuser plate.Red, green, blue three-color light is called the three primary colors of light, if by red, green, blue three kinds of coloured light are pressed different ratios and are mixed, the technical program adopts a circular luminous array of several all-colour LED composition, then by PWM(pulse-width modulation) light regulating technology is red to all all-colour LEDs, green, the intensity of illumination of Lan Sanse is carried out linear regulation, by regulating the proportion of three kinds of colors, thereby blend different backlight colors, then by IMAQ millions pixel high definition CCD camera part, bacterium colony sample is carried out to pattern collection under backlight and can produce except red this, green, any background color beyond blue three-color.
The beneficial effect of the technical program is as follows: adopt polychromatic light lighting system can provide multiple different bacterium colony to gather background, improve the acquisition quality of bacterium colony image, the isolate pairing of different colours with it different light source background can improve the acquisition quality of bacterium colony image, the recognition speed of image software.If cultured bacterium colony primary colours are white, if adopt traditional white background illuminating source, the bacterium colony picture quality of collection and the identification degree of bacterium colony are not high, and it is white bacterium colony that other background colors that adopt polychromatic source to provide can well set off out primary colours.Full-color LED lamp is distributed on light guiding plate surrounding.LED lamp circuit board is connected with master control board by circuit.
Described detection storehouse automatically-controlled door part is made up of glass door, glass clamp, rotating base, grooved light idol, stepper-motor, motor mount, roller, shaft coupling, lower flange, inductive needle and back up pad.Glass door is fixed on glass clamp, and glass clamp is arranged on rotating base.Stepper-motor is arranged on motor mount, and mount pad is arranged on the bottom base of housing parts, and motor is connected with lower flange by shaft coupling, and lower flange is connected on rotating base.Inductive needle becomes 180 ° of two of installations, and in rotating base bottom, roller is arranged on rotating base sidepiece, and rotating base is supported in back up pad, and grooved light is even to be arranged in back up pad, and back up pad is arranged on housing parts stage casing projection.
Stepper-motor action passes to rotating base by shaft coupling, lower flange, makes rotating base drive glass door to be rotated, and the roller of installing on rotating base makes the spin friction power of glass door little, and rotation is smooth.The inductive needle of installing on revolving door is even by grooved light, and the suddenly feel position of required inductive needle of grooved light, positions revolving door.When starting position, glass door closure, 180 ° of stepper-motor forwards, when inductive needle is positioned at the grooved light idol of glass door middle-end by vertical position, bacterial colony counting instrument opens a position; 180 ° of stepper-motor reversions, when inductive needle is positioned at the grooved light idol of glass door starting point end by vertical position, bacterial colony counting instrument closes storehouse.
Described circuit part also comprises supply socket, motor drive plate, switch power supply and power switch.Touch display screen is arranged on shell front end convenient operation and observation, and supply socket and power switch are arranged on shell trailing flank, and master control board, motor drive plate and switch power supply are arranged on housing parts base plate.
Described housing parts comprises upper cover, cabinet, base, signaling interface, bonnet and lower margin.Upper cover can be opened the convenient IMAQ millions pixel high definition CCD camera part of installing, and bonnet can be opened and be convenient to breakdown maintenance, and signaling interface is convenient to image transfer.
Advantage of the present invention is to detect storehouse automatically-controlled door machineryization, automatization, the folding of step motor control door, and slot optical coupling secondary location, safe and reliable to operation, alleviate operator's labour intensity, improve working efficiency.
Accompanying drawing explanation
Fig. 1 is the stereoscopic figure of Automatic Colony Counter of the present invention.
Fig. 2 is the stereoscopic figure of Automatic Colony Counter of the present invention, is convenient to observe remove upper cover and bonnet.
Fig. 3 is the front view of Automatic Colony Counter of the present invention.
Fig. 4 is detection of the present invention storehouse automatically-controlled door component diagram, is convenient to observe remove glass door and buffer bar.
Fig. 5 is light-source system component diagram of the present invention.
Fig. 6 is the workflow diagram of Automatic Colony Counter of the present invention.
1, touch display screen; 2, signaling interface; 3, supply socket; 4, bonnet; 5, switch power supply; 6, upper cover; 7, upper flange back up pad; 8, Camera fixing seat; 9, CCD camera; 10, cabinet; 11, glass door; 12, base; 13, rotating base; 14, lower flange; 15, stepper-motor; 16, motor mount; 17, master control board; 18, lower margin; 19, back up pad; 20, roller; 21, camera mounting flange; 22, glass clamp; 23, inductive needle; 24, grooved light is even; 25, motor drive plate; 26, frosted plate; 27, clamping sleeve; 28, LED lamp circuit board; 29, light guiding plate; 30, full-color LED lamp; 31, diffuser plate
Embodiment
Below in conjunction with the drawings and specific embodiments, structure composition of the present invention and principle of work are further described in detail.
Automatic Colony Counter provided by the invention comprises housing parts, circuit part, IMAQ part and detects storehouse automatically-controlled door part, wherein circuit part comprises touch display screen 1 and master control board 17, and IMAQ part comprises IMAQ millions pixel high definition CCD camera part and light-source system part.Consult accompanying drawing, IMAQ millions pixel high definition CCD camera section is divided and is comprised CCD camera 9, Camera fixing seat 8, camera mounting flange 21.CCD camera 9 is arranged on Camera fixing seat 8, and Camera fixing seat 8 connects with camera mounting flange 21, and camera mounting flange 21 is connected in shell cabinet 10 upper boss.CCD camera 9 is by connection to master control board 17, and master control board 17, can be regulated the flexible of CCD camera 9 camera lenses by the operation of touch display screen 1, thereby complete the adjusting to CCD camera 9 camera lens magnifications by connection to touch display screen 1.
Described light-source system part comprises full-color LED lamp 30, LED lamp circuit board 28, light guiding plate 29, diffuser plate 31, clamping sleeve 27 and frosted plate 26.Full-color LED lamp 30 is uniform to be arranged on LED lamp circuit board 28, and light guiding plate 29 and diffuser plate 31 are arranged on LED lamp circuit board 28, and frosted plate 26 is arranged on rotating base 13, and clamping sleeve 27 is arranged on rotating base 13 and compresses frosted plate 26.Full-color LED lamp 30 is distributed on light guiding plate 29 surroundings, and after full-color LED lamp 30 energisings, light is spread in the detection storehouse of sealing uniformly by light guiding plate 29 and diffuser plate 31.LED lamp circuit board 28 is connected with master control board 17 by circuit, by regulating full-color LED lamp 30 RGB(red, green, blues) ratio, all-colour LED provides multiple illumination background to instrument.
Detect storehouse automatically-controlled door part and comprise that glass door 11, glass clamp 22, rotating base 13, grooved light idol 24, stepper-motor 15, motor mount 16, roller 20, lower flange 14, inductive needle 23 and back up pad 19 form.Glass door 11 is fixed on glass clamp 22, and glass clamp 22 is arranged on rotating base 13.Stepper-motor 15 is arranged on motor mount 16, and mount pad is arranged on the bottom base 12 of housing parts, and stepper-motor 15 is connected with lower flange 14, and lower flange 14 is connected on rotating base 13.In rotating base 13 bottoms, roller 20 is arranged on rotating base 13 sidepieces to 23 one-tenth 180 ° two of installations of inductive needle, and rotating base 13 is supported in back up pad 19, and grooved light even 24 is arranged in back up pad 19, and back up pad 19 is arranged on housing parts cabinet 10 stage casing projections.
Stepper-motor 15 moves and passes to rotating base 13 by lower flange 14, makes rotating base 13 drive glass door 11 to be rotated, and the roller 20 of installing on rotating base 13 makes the spin friction power of glass door 11 little, and rotation is smooth.The inductive needle 23 of installing on revolving door is by grooved light idol 24, and grooved light even 24 senses the position of inductive needle 23, and revolving door is positioned.When starting position, glass door 11 closures, 180 ° of stepper-motor 15 forwards, inductive needle 23 is positioned at even 24 o'clock of the grooved light of glass door middle-end by vertical position, and bacterial colony counting instrument opens a position; Stepper-motor 15 reverses 180 °, and inductive needle 23 is positioned at even 24 o'clock of the grooved light of glass door starting point end by vertical position, and bacterial colony counting instrument closes storehouse.
Circuit part also comprises touch supply socket 3, motor drive plate 25, switch power supply 5 and power switch.Touch display screen 1 is arranged on shell front end convenient operation and observation, and supply socket 3 and power switch are arranged on shell trailing flank, and master control board 17, motor drive plate 25 and switch power supply 5 are arranged on housing parts base 12 base plates.
Housing parts comprises upper cover 6, cabinet 10, base 12, signaling interface 2, bonnet 4 and lower margin 18.Upper cover 6 can be opened convenient IMAQ millions pixel high definition CCD camera 9 parts of installing, and bonnet 4 can be opened and be convenient to breakdown maintenance, and signaling interface 2 is convenient to image transfer.
As mentioned above, given scheme content, can derive similar technical scheme in conjunction with the accompanying drawings and embodiments.In every case be the content that does not depart from technical solution of the present invention, any simple modification, equivalent variations and the modification above embodiment done according to technical spirit of the present invention, all still belong in the scope of technical solution of the present invention.
Claims (6)
1. an Automatic Colony Counter, comprise housing parts, circuit part, IMAQ part and detect storehouse automatically-controlled door part, described circuit part comprises touch display screen and master control board, it is characterized in that: described IMAQ part comprises IMAQ millions pixel high definition CCD camera part and light-source system part, described circuit part, detection storehouse automatically-controlled door part are installed on the base plate of described housing parts.
2. a kind of Automatic Colony Counter as claimed in claim 1, it is characterized in that described IMAQ millions pixel high definition CCD camera section is divided comprises CCD camera, Camera fixing seat, camera mounting flange, CCD camera is arranged on Camera fixing seat, Camera fixing seat connects with camera mounting flange, camera mounting flange is connected on shell upper boss, CCD camera by connection to master control board, master control board by connection to touch display screen.
3. a kind of Automatic Colony Counter as claimed in claim 1 or 2, it is characterized in that described light-source system part comprises full-color LED lamp, LED lamp circuit board, light guiding plate, diffuser plate, clamping sleeve and frosted plate, full-color LED lamp is uniform to be arranged on LED lamp circuit board, light guiding plate and diffuser plate are arranged on LED lamp circuit board, frosted plate is arranged on rotating base, clamping sleeve is arranged on rotating base and compresses frosted plate, full-color LED lamp is distributed on light guiding plate surrounding, and LED lamp circuit board is connected with master control board by circuit.
4. a kind of Automatic Colony Counter as claimed in claim 1 or 2, it is characterized in that described detection storehouse automatically-controlled door part is by glass door, glass clamp, rotating base, grooved light is even, stepper-motor, motor mount, roller, shaft coupling, lower flange, inductive needle and back up pad composition, glass door is fixed on glass clamp, glass clamp is arranged on rotating base, stepper-motor is arranged on motor mount, mount pad is arranged on the bottom base of housing parts, motor is connected with lower flange by shaft coupling, lower flange is connected on rotating base, inductive needle becomes 180 ° to install two in rotating base bottom, roller is arranged on rotating base sidepiece, rotating base is supported in back up pad, grooved light is even to be arranged in back up pad, back up pad is arranged on housing parts stage casing projection.
5. a kind of Automatic Colony Counter as claimed in claim 1 or 2, it is characterized in that described circuit part also comprises supply socket, motor drive plate, switch power supply and power switch, touch display screen is arranged on shell front end, supply socket and power switch are arranged on shell trailing flank, and master control board, motor drive plate and switch power supply are arranged on housing parts base plate.
6. a kind of Automatic Colony Counter as claimed in claim 1 or 2, is characterized in that described housing parts comprises upper cover, cabinet, base, signaling interface, bonnet and lower margin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210399091.1A CN103773679A (en) | 2012-10-19 | 2012-10-19 | Automatic bacterial colony counter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210399091.1A CN103773679A (en) | 2012-10-19 | 2012-10-19 | Automatic bacterial colony counter |
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| Publication Number | Publication Date |
|---|---|
| CN103773679A true CN103773679A (en) | 2014-05-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210399091.1A Pending CN103773679A (en) | 2012-10-19 | 2012-10-19 | Automatic bacterial colony counter |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103983645A (en) * | 2014-05-11 | 2014-08-13 | 王新华 | Novel medical equipment bacteria tester |
| CN104293660A (en) * | 2014-09-10 | 2015-01-21 | 杜晨林 | Detection system for flora detection in food substance |
| CN105491279A (en) * | 2015-11-19 | 2016-04-13 | 北京工业大学 | Image acquisition, recognizing and counting method for colonies |
| CN107629954A (en) * | 2017-11-10 | 2018-01-26 | 广东恒畅环保节能检测科技有限公司 | A kind of colonometer |
| CN111269815A (en) * | 2020-02-12 | 2020-06-12 | 贵州理工学院 | Bacteria calculation image acquisition device |
| CN111286523A (en) * | 2020-02-20 | 2020-06-16 | 中国水产科学研究院黄海水产研究所 | Three-primary-color identification and counting method for bacteria |
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| EP1074610A1 (en) * | 1997-12-02 | 2001-02-07 | Francisco Soria Melguizo, S.A. | System for analyzing images produced by bacterial reactions |
| CN1826521A (en) * | 2003-07-19 | 2006-08-30 | 数字生物技术公司 | Device for counting micro particles |
| CN101622522A (en) * | 2007-01-26 | 2010-01-06 | 贝克顿·迪金森公司 | The method, system and the composition that are used for cell count and analysis |
| CN201804346U (en) * | 2010-06-03 | 2011-04-20 | 合肥瑞韬电子科技有限公司 | Full automatic colony counter |
| CN102089418A (en) * | 2008-04-09 | 2011-06-08 | 耐克思乐生物科学 | Systems and methods for counting cells and biomolecules |
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2012
- 2012-10-19 CN CN201210399091.1A patent/CN103773679A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| EP1074610A1 (en) * | 1997-12-02 | 2001-02-07 | Francisco Soria Melguizo, S.A. | System for analyzing images produced by bacterial reactions |
| US6642953B1 (en) * | 1997-12-02 | 2003-11-04 | Francisco Soria Melguizo, S.A. | System for analyzing images produced by bacterial reactions |
| CN1826521A (en) * | 2003-07-19 | 2006-08-30 | 数字生物技术公司 | Device for counting micro particles |
| CN101622522A (en) * | 2007-01-26 | 2010-01-06 | 贝克顿·迪金森公司 | The method, system and the composition that are used for cell count and analysis |
| CN102089418A (en) * | 2008-04-09 | 2011-06-08 | 耐克思乐生物科学 | Systems and methods for counting cells and biomolecules |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103983645A (en) * | 2014-05-11 | 2014-08-13 | 王新华 | Novel medical equipment bacteria tester |
| CN103983645B (en) * | 2014-05-11 | 2017-05-17 | 赵晓莉 | Novel medical equipment bacteria tester |
| CN104293660A (en) * | 2014-09-10 | 2015-01-21 | 杜晨林 | Detection system for flora detection in food substance |
| CN105491279A (en) * | 2015-11-19 | 2016-04-13 | 北京工业大学 | Image acquisition, recognizing and counting method for colonies |
| CN107629954A (en) * | 2017-11-10 | 2018-01-26 | 广东恒畅环保节能检测科技有限公司 | A kind of colonometer |
| CN111269815A (en) * | 2020-02-12 | 2020-06-12 | 贵州理工学院 | Bacteria calculation image acquisition device |
| CN111286523A (en) * | 2020-02-20 | 2020-06-16 | 中国水产科学研究院黄海水产研究所 | Three-primary-color identification and counting method for bacteria |
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Application publication date: 20140507 |