CN103759978A - Multi-combined device for sterile collection of plant root exudates and application thereof - Google Patents
Multi-combined device for sterile collection of plant root exudates and application thereof Download PDFInfo
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- CN103759978A CN103759978A CN201410047225.2A CN201410047225A CN103759978A CN 103759978 A CN103759978 A CN 103759978A CN 201410047225 A CN201410047225 A CN 201410047225A CN 103759978 A CN103759978 A CN 103759978A
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Abstract
The invention belongs to the technical field of agriculture production devices and particularly relates to a multi-combined device for the sterile collection of plant root exudates and application thereof. The device for the sterile collection of the plant root exudates consists of a sterile air preparing part, a plant culture part and a plant root exudate collecting part, wherein the sterile air preparing part comprises an inflator pump, an air purifying wash bottle, a connecting pipe, an air quantity regulating valve, a check valve, a circular air stone and the like; the plant culture part consists of a cultivating barrel, a porous cork, a glass pipe and a cotton layer; in the plant root exudate collecting part, a nutrient solution is prepared by distilled water and sterilized under high pressure before use, and the plant root exudates are collected by adopting a resin collecting column after a plant stops growing. The device has the characteristics of simple structure, small occupied area, low cost and high sterile level. The device not only is suitable for the sterile collection of the hydroponic plant root exudates, but also is suitable for the preparation of sterile air. A novel modern device is provided for agriculture production.
Description
Technical field
The invention belongs to agricultural production device technique field, be specifically related to the aseptic gathering-device of a kind of multi-joint secretions from plant roots and application thereof.
Background technology
Soil is the medium of plant growth, is the material base that the mankind depend on for existence, is the main source of the required moisture of plant, heat and nutriment.The quality of soil quality is directly connected to the quality safety of agricultural product, the mankind's healthy and socioeconomic sustainable development.In recent years, along with the increase of the soil continuous cropping cultivation time limit, occurred that soil quality deterioration, sick worm are serious, a series of phenomenons of degradation under crop yield reduction, quality of agricultural product.What Chinese scholars was found root system of plant through years of researches may be a main cause that causes soil quality degeneration and continuous cropping obstacle from poison.The reciprocation of Plants and Soils and soil microorganism is also an important directions in following soil quality research, for this reason, the gathering-device of many moneys root exudates has been manufactured in domestic many laboratories invention, as the original position gathering-device of the secretions from plant roots of Agricultural University Of South China's invention, the gathering-device of Lanzhou University potted plant, the in-situ root system secretion gathering-device of University Of Shenyang, the apparatus for gathering combined plant root system secretion of Northeast Agricultural University, the secretions from plant roots harvester of Shanghai University etc., the common feature of these root exudates is excretion collecting device for root system of plant complicated operations, can not once collect in a large number the secretions from plant roots complicated component problem of secretions from plant roots and collection.Simultaneously, once test required device many, the consistance of the experimental data between device there are differences, and secretions from plant roots also easily adsorbs by soil colloid in flow process, and the metabolic process of soil microorganism also can impact the composition of root exudates.
Summary of the invention
The technical issues that need to address of the present invention are for the deficiencies in the prior art, and a kind of easy to use, practical, multi-joint root exudates gathering-device and application thereof is cheaply provided.
Technical scheme of the present invention is that multi-joint root exudates gathering-device is cultivated partly by filtrated air preparation part, plant and secretions from plant roots collection unit is grouped into.Filtrated air preparation part is comprised of time controller, inflator pump, air purification bottle, connecting pipe, air volume regulating valve, the gentle huge rock of retaining valve; Plant is cultivated part and is comprised of cultivation bucket, porous cork, glass tube, cotton layer, by gas huge rock, for root system of plant provides required aseptic oxygen, utilizes the isolated outside contamination of cotton layer source to enter aseptic culture system; Secretions from plant roots is collected part, and nutrient solution is prepared with distilled water, autoclaving before using, and plant growth finishes rear employing resin and collects post collection root exudates.The innovative point of this device is:
1, the collection of secretions from plant roots is carried out under germ-free condition.By the asepticize of plant root growth environment is processed, root system of plant is grown all the time under germ-free condition; Nutrient solution carries out asepticize processing by autoclaved mode; The needed oxygen of root growth provides by aseptic gas generation system; Utilize cotton to prevent that in culture apparatus, undressed air enters in plant root growth environment; Utilize retaining valve to control the flow direction of gas, and prevent liquid backflow.
2, to breathe required oxygen be to provide by filtrated air preparation system to root system of plant.Utilize the adjustable inflator pump air feed of tolerance, first pass through the particulate matter in cotton layer filtering gas, recycling sulfuric acid is removed airborne microorganism, after utilizing activated charcoal to sulfuric acid treatment after allowing, airborne acid and other impurity adsorb, gained filtrated air produces bubble by the gentle huge rock of wireway, for growing plants root system in nutrient solution provides oxygen.
3, this device is a kind of multi-joint root exudates collection system.What is called is multi-joint is to utilize the air that part flow arrangement is prepared filtrated air system all to assign in a plurality of aseptic culture devices, has overcome the error existing between different filtrated air preparation facilities, and has greatly saved test space and equipment investment.
4, root system of plant asepsis growth environment is by nutrient solution is carried out to autoclaving, utilize the isolated outside contamination of the sealing source of cotton layer and culture apparatus to enter root growth system, utilize filtrated air preparation system to provide root system respiration required oxygen, guarantee that plant root growth environment is in germ-free condition.
5, be to control air demand by controlling the air volume regulating valve of inflator pump dividing equally of aseptic gas, utilizes the Air-regulating valves on part flow arrangement to regulate the air demand of each aseptic culture system, and then reach the consistance that obtains amount of oxygen in each aseptic culture system.
The present invention compared with prior art its beneficial effect is:
Ingenious, the science of this apparatus structure design, filtrated air preparation system, plant cultivation system and secretions from plant roots are collected to concentration systems combination cleverly, for the reciprocation research of the research of plant soil reciprocation, secretions from plant roots and soil microorganism and the research of other association area provide necessary condition.
This apparatus structure is simple, floor area is little, cost is low, aseptic level is high, has both been suitable for the aseptic collection of water culture root exudates, is also suitable for the preparation of filtrated air.This device can be used for the research of Plants and Soils reciprocation or plant and the research of rhizosphere microorganism reciprocation or secretions from plant roots poison research or soil quality detection certainly.Meanwhile, also can be applicable to carry out other research field of filtrated air.For agricultural production provides a kind of new modernization device.
Accompanying drawing explanation
The aseptic gathering-device of the multi-joint secretions from plant roots of Fig. 1
(1) the unidirectional variable valve of inflator pump (2) air volume regulating valve (3) (4) cotton layer (5) sulfuric acid Drexel bottle (6) activated charcoal Drexel bottle (7) wireway (8) cultivation bucket (9) air volume regulating valve (10) culture hole (11) liquid filling hole (12) gas huge rock (13) porous cork (14) plant nutrient (15) part flow arrangement (16) air admission hole (17) cotton layer
Embodiment
1, the plant nutrition liquid after the interior Sheng of cultivation bucket (8) autoclaving is (14) 1.2 liters, on porous cork (13), there are two apertures (11,16) be 6mm, insert ventilation glass tube, aperture 11 cotton jam-pack, aperture 16 connects the inside and outside wireway (7) of cultivation bucket, and an aperture (10) is 15mm, as culture hole, embed glass tube.
2, after vegetable seeds sterilization, vernalization under germ-free condition; Before sprigging, whole plant all grows under germ-free condition; When transplanting, plant first uses sterile water wash root system 3-5 time.
3, on superclean bench by the quick definite value of aseptic seedlings plant in culture hole (10), use cotton jam-pack, cork (13) external application cotton (17) is tightened, in the controlled environment chamber, cultivate, daytime 14h/28 ℃, night 10h/25 ℃, with glass tube, connect the wireway (7) on gas huge rock (12) and part flow arrangement (15), the air passing into need to pass through sulfuric acid Drexel bottle (5) and activated charcoal Drexel bottle (6), and cotton layer (4) filtration, to reduce pollution.
4, by the air volume regulating valve (2) of inflator pump and the air volume regulating valve (9) of part flow arrangement, control aeration quantity, guarantee that each cultivation bucket throughput is consistent, by unidirectional variable valve (3), control the flow direction of gas, utilize the time relay to control inflator pump (1), inflation 40min, stop 20min, cycle control.
5, during plant growth, according to the consumption of nutrient solution in plant strain growth and cultivation bucket, from liquid filling hole (11), supplement without bacterial nutrient solution, after transplanting, growth is 20 days, take out plant, the solution staying is used without bacterial nutrient solution and is supplemented to original volume, adopts resin to collect post and collect root exudates after bacteria filter filters.
6, in cultivation bucket, establish blank bucket, except underrange plant, other condition is consistent with cultivation plant bucket.In root exudates constituent analysis in contrast.
Claims (3)
1. the aseptic gathering-device of multi-joint secretions from plant roots, is characterized in that being grouped into by filtrated air preparation part, plant cultivation part and secretions from plant roots collection unit.
2. the aseptic gathering-device of a kind of multi-joint secretions from plant roots according to claim 1, is characterized in that filtrated air preparation part is comprised of time controller, inflator pump, air purification bottle, connecting pipe, air volume regulating valve, the gentle huge rock of retaining valve; Plant is cultivated part and is comprised of cultivation bucket, porous cork, glass tube, cotton layer, by gas huge rock, for root system of plant provides required aseptic oxygen, utilizes the isolated outside contamination of cotton layer source to enter aseptic culture system; Secretions from plant roots is collected part, and nutrient solution is prepared with distilled water, autoclaving before using, and plant growth finishes rear employing resin and collects post collection root exudates.
3. the application of the aseptic gathering-device of a kind of multi-joint secretions from plant roots in the research of Plants and Soils reciprocation or plant and the research of rhizosphere microorganism reciprocation or the certainly malicious research of secretions from plant roots or soil quality detection described in claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410047225.2A CN103759978A (en) | 2014-02-10 | 2014-02-10 | Multi-combined device for sterile collection of plant root exudates and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410047225.2A CN103759978A (en) | 2014-02-10 | 2014-02-10 | Multi-combined device for sterile collection of plant root exudates and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108641922A (en) * | 2018-04-28 | 2018-10-12 | 大连普瑞康生物技术有限公司 | A kind of culture collection device |
| CN109496824A (en) * | 2018-12-29 | 2019-03-22 | 广西壮族自治区农业科学院微生物研究所 | Collect the hydroponic device and ciltivating process of cultivated plant root rhizosphere secreta |
| CN109932229A (en) * | 2019-03-22 | 2019-06-25 | 济南大学 | A kind of collection method of Rhizosphere of Crops secretion |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109496824A (en) * | 2018-12-29 | 2019-03-22 | 广西壮族自治区农业科学院微生物研究所 | Collect the hydroponic device and ciltivating process of cultivated plant root rhizosphere secreta |
| CN109932229A (en) * | 2019-03-22 | 2019-06-25 | 济南大学 | A kind of collection method of Rhizosphere of Crops secretion |
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Application publication date: 20140430 |