CN103734124B - The store method of a kind of orthopedic transplant material - Google Patents
The store method of a kind of orthopedic transplant material Download PDFInfo
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- CN103734124B CN103734124B CN201410000005.4A CN201410000005A CN103734124B CN 103734124 B CN103734124 B CN 103734124B CN 201410000005 A CN201410000005 A CN 201410000005A CN 103734124 B CN103734124 B CN 103734124B
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- transplant material
- orthopedic
- iodine
- conserving liquid
- orthopedic transplant
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Abstract
<b>the present invention discloses the store method of a kind of orthopedic transplant material, carry out according to the following steps: 1) orthopedic transplant material obtained under aseptic condition is directly put into the container containing conserving liquid, after packing, it is put in the refrigeration chamber of common refrigerator and refrigerates, for subsequent use; Wherein, containing the iodine volt that concentration is 1% in conserving liquid; 2) open packaging when using, with physiological saline by the iodine deep-sited part of the chong channel wash clean in orthopedic transplant material, can use. The present invention is without the need to specific installation, and retention period is long, and standing charges are low, immediately available, convenient and swift, can avoid propagating the risk infected simultaneously, be easy to the medical institutions in various rank and promote, can effectively reduce medical expense.</b>
Description
Technical field
The present invention relates to the store method of a kind of orthopedic transplant material, particularly relate to the allogeneic of clinical medicine orthopaedics or the xenograft material sterilizing store method under low temperature environment in vitro.
Background technology
In orthopaedics clinical position, orthopaedics related tissue comprises the tissues such as bone, cartilage, nerve, tendon, ligament, meniscus, can not normal healing after a lot of tissue damage. Allogenic material must be used to transplant carry out repairing or rebuilding. At present, allogenic material store method has freeze-drying, deep-bed drying method or fresh deeply freezes method. Wherein cold dry method be under freezing conditions tissue water content is down to minimum, then tissue can preserve at normal temperatures. But the material of this kind of method process becomes crisp, and mechanical strength obviously lowers. Tendon tissue almost loses toughness completely, still is easy to fracture after rehydration, cannot use. Osseous tissue can use, but after transplanting, new osteanagenesis is very slow.
Deep-bed drying is preserved in the profound hypothermia refrigerator of subzero about 80 by graft materials, and extraordinary refrigerator is expensive, and retention period is short, costly.
Fresh deeply freeze preserve be the tissue without sterilising treatment, after aseptic drawing materials, directly put into profound hypothermia Refrigerator store. This kind of method propagates the risk infected to be increased, and comprises bacterium, fungi, virus (hepatitis, acquired immune deficiency syndrome (AIDS) etc.).
Organizing that above method preserves is expensive, and such as, lyophilize bone reaches every gram about 500 yuan, considerably beyond the price of gold.
Summary of the invention
The shortcoming of complex process, cost costliness is there is for the maintenance method of prior art, it is an object of the invention to provide the store method of a kind of orthopedic transplant material, the method is without the need to specific installation, retention period is long, and standing charges are low, immediately available, convenient and swift, can avoid propagating the risk infected simultaneously, be easy to the medical institutions in various rank and promote, can effectively reduce medical expense.
The object realizing the present invention can by taking following technical scheme to reach:
The store method of a kind of orthopedic transplant material, it is characterised in that carry out according to the following steps:
1) orthopedic transplant material obtained under aseptic condition is directly put into the container containing conserving liquid, after packing, it is put in the refrigeration chamber of common refrigerator and refrigerates, for subsequent use; Wherein, containing the iodine volt that concentration is 1% in conserving liquid;
Described orthopedic transplant material comprises bone, cartilage, meniscus, tendon, ligament or nerve etc.
2) open packaging when using, with physiological saline by the iodine deep-sited part of the chong channel wash clean in orthopedic transplant material, can use.
The object realizing the present invention can also by taking following technical scheme to reach:
Realizing one embodiment of the present invention is: in step 1), and the temperature of refrigeration controls at-4 DEG C-0 DEG C.
Realizing one embodiment of the present invention is: in step 1), in addition it is also necessary to add dimethyl sulfoxide (DMSO) in conserving liquid.
Realizing one embodiment of the present invention is: in step 1), and the add-on of described dimethyl sulfoxide (DMSO) is the 3%-5% of conserving liquid weight.
Realizing one embodiment of the present invention is: step 2) in, before use, by Sulfothiorine, orthopedic transplant material is carried out de-iodine.
The useful effect of the present invention is:
The present invention uses common iodine volt liquid, common refrigerator, without the need to specific installation, retention period is long, it is possible to preserve 5 years, standing charges are low, immediately available, convenient and swift, can avoid propagating the risk infected simultaneously, carry out the donation of orthopaedics related tissue by making national hospital at different levels have ready conditions, greatly reduce associated cost. Store method of the present invention, is applicable to the tissue of excision in performing the operation, such as the femoral head of excision during artificial hip joint replacing, the sclerotin of excision, meniscus and ligamentaum cruciatum during knee prosthesis, the limbs institute band tissue of amputation. Can also be used for preserving the various orthopaedics related tissue of cadaver donor.
Embodiment
Below, in conjunction with embodiment, the present invention is described further:
Embodiment 1:
A store method for orthopedic transplant material, carries out according to the following steps:
1) orthopedic transplant material obtained under aseptic condition is directly put into the container containing conserving liquid, after packing, it is put in the refrigeration chamber of common refrigerator and refrigerates, for subsequent use; Wherein, containing the iodine volt that concentration is 1% in conserving liquid; The temperature of refrigeration controls at 0 DEG C.
2) open packaging when using, with physiological saline by the iodine deep-sited part of the chong channel wash clean in orthopedic transplant material, can use.
Embodiment 2:
A store method for orthopedic transplant material, carries out according to the following steps:
1) orthopedic transplant material obtained under aseptic condition is directly put into the container containing conserving liquid, after packing, it is put in the refrigeration chamber of common refrigerator and refrigerates, for subsequent use; Wherein, containing the iodine volt that concentration is 1% in conserving liquid; The temperature of refrigeration controls at-2 DEG C.
2) open packaging when using, with physiological saline by the iodine deep-sited part of the chong channel wash clean in orthopedic transplant material, can use.
Embodiment 3:
A store method for orthopedic transplant material, carries out according to the following steps:
1) orthopedic transplant material obtained under aseptic condition is directly put into the container containing conserving liquid, after packing, it is put in the refrigeration chamber of common refrigerator and refrigerates, for subsequent use; Wherein, containing the iodine volt that concentration is 1% in conserving liquid; The temperature of refrigeration controls at-4 DEG C.
2) open packaging when using, with physiological saline by the iodine deep-sited part of the chong channel wash clean in orthopedic transplant material, can use.
Embodiment 4:
The feature of the present embodiment is: in step 1), in addition it is also necessary to add dimethyl sulfoxide (DMSO) in conserving liquid. The add-on of described dimethyl sulfoxide (DMSO) is the 3% of conserving liquid weight. Other are identical with specific embodiment 1.
Embodiment 5:
The feature of the present embodiment is: in step 1), in addition it is also necessary to add dimethyl sulfoxide (DMSO) in conserving liquid. The add-on of described dimethyl sulfoxide (DMSO) is the 5% of conserving liquid weight. Other are identical with specific embodiment 1.
Embodiment 6:
The feature of the present embodiment is: step 2) in, before use, by Sulfothiorine, orthopedic transplant material is carried out de-iodine. Other are identical with specific embodiment 1.
For a person skilled in the art, according to technical scheme described above and design, other various corresponding change and distortion can be made, and all these change and are out of shape within the protection domain that all should belong to the claims in the present invention.
Claims (1)
1.The store method of a kind of orthopedic transplant material, it is characterised in that carry out according to the following steps:
1) orthopedic transplant material obtained under aseptic condition is directly put into the container containing conserving liquid, after packing, it is put in the refrigeration chamber of common refrigerator and refrigerates, for subsequent use; Wherein, containing the iodine volt that concentration is 1% in conserving liquid;
2) open packaging when using, with physiological saline by the iodine deep-sited part of the chong channel wash clean in orthopedic transplant material, can use;
In step 1), the temperature of refrigeration controls at-4 DEG C-0 DEG C;
In step 1), in addition it is also necessary to add dimethyl sulfoxide (DMSO) in conserving liquid;
In step 1), the add-on of described dimethyl sulfoxide (DMSO) is the 3%-5% of conserving liquid weight;
Step 2) in, before use, by Sulfothiorine, orthopedic transplant material is carried out de-iodine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410000005.4A CN103734124B (en) | 2014-01-01 | 2014-01-01 | The store method of a kind of orthopedic transplant material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410000005.4A CN103734124B (en) | 2014-01-01 | 2014-01-01 | The store method of a kind of orthopedic transplant material |
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| Publication Number | Publication Date |
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| CN103734124A CN103734124A (en) | 2014-04-23 |
| CN103734124B true CN103734124B (en) | 2016-06-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410000005.4A Expired - Fee Related CN103734124B (en) | 2014-01-01 | 2014-01-01 | The store method of a kind of orthopedic transplant material |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112042636B (en) * | 2020-09-02 | 2022-05-13 | 江西省元化低温医学科技有限公司 | Preservation method of autologous skull flap containing rhBMP-2 and periosteum |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1528469A (en) * | 2003-10-09 | 2004-09-15 | 中国人民解放军南京军区南京总医院 | Method for preparing and preserving homogeneous bone tissue with biological activity |
| WO2010021714A3 (en) * | 2008-08-20 | 2010-04-15 | Anthrogenesis Corporation | Improved cell composition and methods of making the same |
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2014
- 2014-01-01 CN CN201410000005.4A patent/CN103734124B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1528469A (en) * | 2003-10-09 | 2004-09-15 | 中国人民解放军南京军区南京总医院 | Method for preparing and preserving homogeneous bone tissue with biological activity |
| WO2010021714A3 (en) * | 2008-08-20 | 2010-04-15 | Anthrogenesis Corporation | Improved cell composition and methods of making the same |
Non-Patent Citations (3)
| Title |
|---|
| 人骨软骨组织体外冷冻保存的效果;亓建洪 等;《中国组织工程研究与临床康复》;20111112;第15卷(第46期);参见对比文件2第8580页左栏第3段 * |
| 李媛媛.用硫代硫酸钠清除碘痕迹.《中国误诊学杂志》.2011,第11卷(第21期), * |
| 碘伏浸泡冷冻保存自体颅骨行颅骨修补术临床研究;衡雪源 等;《济宁医学院学报》;20030930;第26卷(第3期);参见对比文件1第64页左栏第3段 * |
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| CN103734124A (en) | 2014-04-23 |
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Granted publication date: 20160601 Termination date: 20180101 |