CN103604502A - Raman spectrometer used for detecting high scattering medium - Google Patents
Raman spectrometer used for detecting high scattering medium Download PDFInfo
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- CN103604502A CN103604502A CN201310587511.3A CN201310587511A CN103604502A CN 103604502 A CN103604502 A CN 103604502A CN 201310587511 A CN201310587511 A CN 201310587511A CN 103604502 A CN103604502 A CN 103604502A
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Abstract
The invention relates to a Raman spectrometer used for detecting a high scattering medium. The Raman spectrometer comprises a laser device, wherein an output light path along the laser device is sequentially provided with a collimation convex lens, a semi-transparency semi-reflecting light splitter, a conical lens and a convergence convex lens, a reflecting light path of the semi-transparency semi-reflecting light splitter is sequentially provided with a film filter, a reception convex lens and a light splitting spectrometer used for receiving a light beam filtered by the film filter, an output end of the light splitting spectrometer is connected with an array CCD detector which is for imaging of the light beam after light splitting of the light splitting spectrometer, and a focus point of the convergence convex lens corresponds to a detected sample. According to the Raman spectrometer, the conical lens is utilized to generate the approximation zero order Bessel light beam to overcome high scattering of the medium, laser loss is reduced, the laser is focused to be a light spot in the high scattering medium, transmission depth of the light beam in the high scattering medium is improved, spectrum dispersion is reduced, and Raman signals at the focus point are collected for spectrum analysis.
Description
Technical field
The present invention relates to a kind of Raman spectrometer.The Raman spectrometer that particularly relates to the high scattering medium of a kind of effective detection.
Background technology
Raman scattering is the inelastic scattering phenomenon that a kind of molecule is subject to photon excitation, Raman Characterization the molecular structure property of chemical substance, can be used for identifying the functional group existing in molecule, realize the fingerprint of chemical molecular and debate knowledge, thereby be used as the effective ways that unknown chemicals are identified in identification.
Raman spectrum has been widely used in the fields such as material, chemical industry, oil, macromolecule, biology, environmental protection, geology at present.
Due to the scattering process of high scattering medium (as biological tissue), make the penetration depth of laser very limited on the one hand, cause the high scattering loss of laser, thereby cause the application restric-tion of existing Raman spectrometer in surface analysis; Make on the other hand Raman spectrum disperse, reduce the accuracy of spectral detection.For analyzing the material under high scattering medium top layer, must increase the penetration depth of laser in high scattering medium, and reduce spectrum disperse.
Summary of the invention
Technical matters to be solved by this invention is, providing a kind of can increase the transmission depth of light beam in high scattering medium, reduces spectrum disperse, and the Raman signal of collecting focus place carries out the Raman spectrometer of the high scattering medium of detection of spectral analysis.
The technical solution adopted in the present invention is: a kind of Raman spectrometer that detects high scattering medium, comprise laser instrument, along being disposed with collimation convex lens on the output light path of described laser instrument, semi-transparent semi-reflecting spectroscope, Conical Lenses and convergence convex lens, on described semi-transparent semi-reflecting spectroscopical reflected light path, be disposed with filter plate, receive the sub-ray spectrometer of convex lens and the light beam of reception after filter plate filtering convex lens focus, the output terminal of described sub-ray spectrometer is connected with the array CCD detecting device that the light beam after sub-ray spectrometer light splitting is carried out to imaging, the corresponding detected sample of focus point of described convergence convex lens.
Described collimation convex lens and the fixed installation of described laser coaxial, the axes intersect of described semi-transparent semi-reflecting spectroscopical axis and described collimation convex lens, described Conical Lenses and assemble convex lens and described collimation convex lens coaxially fixedly mount, the axes intersect of the axis of described filter plate and described collimation convex lens, described reception convex lens and described filter plate coaxially fixedly mount.
The axis angle of described semi-transparent semi-reflecting spectroscopical axis and described collimation convex lens is 45 degree.
The axis angle of described semi-transparent semi-reflecting spectroscopical axis and described filter plate is 45 degree.
The intersect vertical axis of the axis of described collimation convex lens and described filter plate.
A kind of Raman spectrometer that detects high scattering medium of the present invention, utilize Conical Lenses to produce approximate zeroth order bessel beam to overcome the high scattering of medium, reduce laser loss, make it in high scattering medium, be focused into a hot spot, increase the transmission depth of light beam in high scattering medium, reduce spectrum disperse, and the Raman signal of collecting focus place carries out spectral analysis.There is following beneficial effect:
1, reduce spectrum disperse, obtain tested high scatterer Raman spectrum accurately;
2, increase the transmission depth of laser in high scatterer, obtain high scatterer internal excitation spectrum, improve the accuracy that Raman spectrometer detects high scatterer.
Accompanying drawing explanation
Fig. 1 is one-piece construction schematic diagram of the present invention.
In figure
1: laser instrument 2: collimation convex lens
3: semi-transparent semi-reflecting spectroscope 4: Conical Lenses
5: assemble convex lens 6: filter plate
7: receive convex lens 8: sub-ray spectrometer
9: array CCD detecting device 10: detected sample
11: laser beam
Embodiment
Below in conjunction with embodiment and accompanying drawing, a kind of Raman spectrometer that detects high scattering medium of the present invention is described in detail.
As shown in Figure 1, a kind of Raman spectrometer that detects high scattering medium of the present invention, comprise laser instrument 1, along being disposed with collimation convex lens 2 on the output light path of described laser instrument 1, semi-transparent semi-reflecting spectroscope 3, Conical Lenses 4 and convergence convex lens 5, on the reflected light path of described semi-transparent semi-reflecting spectroscope 3, be provided with filter plate 6, receive the sub-ray spectrometer 8 of convex lens 7 and the reception light beam after filter plate 6 filtering convex lens 7 focus on, the output terminal of described sub-ray spectrometer 8 is connected with the light beam after sub-ray spectrometer 8 light splitting is carried out to the array CCD detecting device 9 that imaging obtains Raman spectrum, wherein, the corresponding detected sample 10 of focus point of described convergence convex lens 5.
Described collimation convex lens 2 coaxially fixedly mount with described laser instrument 1, the axes intersect of the axis of described semi-transparent semi-reflecting spectroscope 3 and described collimation convex lens 2, and the axis angle of the axis of described semi-transparent semi-reflecting spectroscope 3 and described collimation convex lens 2 is 45 degree.Described Conical Lenses 4 and convergence convex lens 5 coaxially fixedly mount with described collimation convex lens 2, the axes intersect of the axis of described filter plate 6 and described collimation convex lens 2, axes intersect with described semi-transparent semi-reflecting spectroscope 3, described reception convex lens 7 coaxially fixedly mount with described filter plate 6, and the axis angle of the axis of described semi-transparent semi-reflecting spectroscope 3 and described filter plate 6 is 45 degree.
The position of described filter plate 6 can arrange flexibly, as long as its light beam that measured light can be sent is divided into different measurement wave bands.The axis of the axis of the filter plate 6 described in the present embodiment and described collimation convex lens 2 is for intersecting vertically.
The principle of work of a kind of Raman spectrometer that detects high scattering medium of the present invention is as follows.
Laser instrument 1 produces laser beam 11, and laser beam 11 becomes collimated light beam through collimation convex lens 2, and collimated light beam is incident to Conical Lenses 4 after semi-transparent semi-reflecting spectroscope 3 light splitting.Collimated light beam becomes approximate zeroth order non-diffraction Bessel beam through Conical Lenses 4 from Gaussian beam.It shows as light intensity and the size of beam center hot spot without diffraction characteristic, in a certain limited propagation distance, substantially remain unchanged.When the horizontal dimension of barrier is less than beamwidth, can return to original cross direction profiles, the self-reparability of Here it is Beams.Above characteristic based on bessel beam, bessel beam is applied in the testing process of high scatterer.Bessel beam focuses on detected sample 10 through assembling convex lens 5.Detected sample 10 is high scattering mediums, light beam runs into barrier and is scattered, self-reparability due to bessel beam, make light beam enter material inside, at ideal position, form the focal beam spot with certain depth, and then increase the transmission depth of light beam in high scatterer, the while is due to the self-reparability of bessel beam, the Raman spectrum disperse producing is reduced, improve the accuracy detecting.
Laser beam at focus place is stimulated and produces Raman signal and Rayleigh signal, after being collected, along phase reflective road, through Conical Lenses 4, by semi-transparent semi-reflecting spectroscope 3, is reflected by convergent lens 5, and after filter plate 6 filtering, in Raman signal, Rayleigh scattering light is by filtering.Filtered Raman signal focuses on sub-ray spectrometer 8, imaging on array CCD detecting device 9 after sub-ray spectrometer 8 light splitting through receiving convex lens 7.The structure that the Raman spectrum receiving has accurately disclosed the high scatterer in certain depth of focus place forms information.The position of filter plate 6 can arrange flexibly, as long as its light beam that measured light can be sent is divided into different measurement wave bands.
In a word, so long as structural principle is identical with the present invention, the simple substitute of notification technique, within all dropping on protection scope of the present invention.
Claims (5)
1. a Raman spectrometer that detects high scattering medium, comprise laser instrument (1), it is characterized in that, along being disposed with collimation convex lens (2) on the output light path of described laser instrument (1), semi-transparent semi-reflecting spectroscope (3), Conical Lenses (4) and convergence convex lens (5), on the reflected light path of described semi-transparent semi-reflecting spectroscope (3), be disposed with filter plate (6), receive the sub-ray spectrometer (8) of convex lens (7) and the light beam of reception after filter plate (6) filtering convex lens (7) focus on, the output terminal of described sub-ray spectrometer (8) is connected with the array CCD detecting device (9) that the light beam after sub-ray spectrometer (8) light splitting is carried out to imaging, the corresponding detected sample (10) of focus point of described convergence convex lens (5).
2. a kind of Raman spectrometer that detects high scattering medium according to claim 1, it is characterized in that, described collimation convex lens (2) coaxially fixedly mount with described laser instrument (1), the axes intersect of the axis of described semi-transparent semi-reflecting spectroscope (3) and described collimation convex lens (2), described Conical Lenses (4) and convergence convex lens (5) coaxially fixedly mount with described collimation convex lens (2), the axes intersect of the axis of described filter plate (6) and described collimation convex lens (2), described reception convex lens (7) coaxially fixedly mount with described filter plate (6).
3. a kind of Raman spectrometer that detects high scattering medium according to claim 2, is characterized in that, the axis angle of the axis of described semi-transparent semi-reflecting spectroscope (3) and described collimation convex lens (2) is 45 degree.
4. a kind of Raman spectrometer that detects high scattering medium according to claim 2, is characterized in that, the axis angle of the axis of described semi-transparent semi-reflecting spectroscope (3) and described filter plate (6) is 45 degree.
5. a kind of Raman spectrometer that detects high scattering medium according to claim 5, is characterized in that, the intersect vertical axis of the axis of described collimation convex lens (2) and described filter plate (6).
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103854305A (en) * | 2014-03-19 | 2014-06-11 | 天津大学 | Module transfer method based on multiscale modeling |
| CN104749162A (en) * | 2015-04-17 | 2015-07-01 | 江苏天瑞仪器股份有限公司 | Confocal Raman spectrometer and light path device thereof |
| CN106023082A (en) * | 2016-05-24 | 2016-10-12 | 哈尔滨工业大学 | Microlens array and pulse laser based dispersion medium optical parameter field reconstruction device and method |
| CN106338501A (en) * | 2016-03-23 | 2017-01-18 | 北京杏林睿光科技有限公司 | Novel Raman probe with light source |
| CN107356581A (en) * | 2017-08-02 | 2017-11-17 | 中国科学院苏州生物医学工程技术研究所 | The Raman spectrometer of full depth far end scan |
| CN107478332A (en) * | 2017-08-03 | 2017-12-15 | 北京理工大学 | A kind of burnt longitudinal high-resolution imaging device of annular beam copolymerization |
| CN109297949A (en) * | 2018-09-19 | 2019-02-01 | 珠海彩晶光谱科技有限公司 | The tumour cell detection method and device of micro-imaging combination transmission Raman spectrum |
| CN111213040A (en) * | 2018-08-10 | 2020-05-29 | 合刃科技（深圳）有限公司 | Vibration monitoring method and system |
| CN111413315A (en) * | 2020-03-31 | 2020-07-14 | 西安电子科技大学 | Probe type Raman spectrometer based on Bessel light |
| CN111413314A (en) * | 2020-03-31 | 2020-07-14 | 西安电子科技大学 | Portable Raman spectrometer based on Bessel light |
| CN113433065A (en) * | 2021-06-16 | 2021-09-24 | 北京大学 | Turbulent flow spectrum measurement system based on Bessel CARS and measurement method thereof |
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| JP2013152192A (en) * | 2012-01-26 | 2013-08-08 | Tokyo Univ Of Science | Organic compound analyzer and organic compound analysis method |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103854305A (en) * | 2014-03-19 | 2014-06-11 | 天津大学 | Module transfer method based on multiscale modeling |
| CN104749162A (en) * | 2015-04-17 | 2015-07-01 | 江苏天瑞仪器股份有限公司 | Confocal Raman spectrometer and light path device thereof |
| CN104749162B (en) * | 2015-04-17 | 2018-03-27 | 江苏天瑞仪器股份有限公司 | Confocal laser-scanning microscopy instrument and its light path device |
| CN106338501A (en) * | 2016-03-23 | 2017-01-18 | 北京杏林睿光科技有限公司 | Novel Raman probe with light source |
| CN106023082A (en) * | 2016-05-24 | 2016-10-12 | 哈尔滨工业大学 | Microlens array and pulse laser based dispersion medium optical parameter field reconstruction device and method |
| CN106023082B (en) * | 2016-05-24 | 2019-01-08 | 哈尔滨工业大学 | Dispersive medium optical parameter field reconstructing device and its method for reconstructing based on microlens array and pulse laser |
| CN107356581A (en) * | 2017-08-02 | 2017-11-17 | 中国科学院苏州生物医学工程技术研究所 | The Raman spectrometer of full depth far end scan |
| CN107478332A (en) * | 2017-08-03 | 2017-12-15 | 北京理工大学 | A kind of burnt longitudinal high-resolution imaging device of annular beam copolymerization |
| CN111213040A (en) * | 2018-08-10 | 2020-05-29 | 合刃科技（深圳）有限公司 | Vibration monitoring method and system |
| CN109297949A (en) * | 2018-09-19 | 2019-02-01 | 珠海彩晶光谱科技有限公司 | The tumour cell detection method and device of micro-imaging combination transmission Raman spectrum |
| CN109297949B (en) * | 2018-09-19 | 2024-04-05 | 上海镭立激光科技有限公司 | Tumor cell detection method and device by combining microscopic image with transmission Raman spectrum |
| CN111413315A (en) * | 2020-03-31 | 2020-07-14 | 西安电子科技大学 | Probe type Raman spectrometer based on Bessel light |
| CN111413314A (en) * | 2020-03-31 | 2020-07-14 | 西安电子科技大学 | Portable Raman spectrometer based on Bessel light |
| CN113433065A (en) * | 2021-06-16 | 2021-09-24 | 北京大学 | Turbulent flow spectrum measurement system based on Bessel CARS and measurement method thereof |
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| CN103604502B (en) | 2016-02-03 |
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