CN103476256B - SDHI fungicide is used for the stem canker resistance of ASR tolerance and/or the purposes of frogeye leaf spot resistant soybean kind of conventional breeding - Google Patents

Abstract

The present invention relates to the method for the stem canker resistance of the ASR tolerance for preventing and treating conventional breeding and/or the Asian Soybean rest fungus (ASR) of frogeye leaf spot resistant soybean kind, comprising and succinate dehydrogenase inhibitors class (SDHI) fungicide is applied to plant, plant propagation material or its growth place.

Description

SDHI fungicide is used for the stem canker resistance of ASR tolerance and/or the purposes of frogeye leaf spot resistant soybean kind of conventional breeding

The present invention relates to the control stem canker resistance of Asian Soybean rest fungus (ASR) tolerance of conventional breeding and/or the method for the ASR of frogeye leaf spot resistant soybean kind, comprise and succinate dehydrogenase inhibitors class (SDHI) fungicide is applied to described plant, plant propagation material or its growth place.

Soybean (soybean (Glycine) genus) is considered to important crop and is subject to the great attention of world agriculture.Therefore, one of soybean breeder person main object is to develop more stable, fecund and disease-resistant kind.Basic motive grain yield is maximized consume for human and animal.For realizing described object, breeder selects the kind with outstanding speciality usually.

Asian Soybean rest fungus (ASR) is caused by fungi soybean rest fungus (Phakopsorapachyrhizi), is considered to beans leaf disease evil (Miles, the M.R. of most destructive power; FrederickR.D.; Hartman, G. (2003) Soybeanrust:IstheU.S.soybeancropatrisk Online.APSnetFeature, AmericanPhytopathologicalSociety).This disease is spread by the uredospore of wind, is transmitted to the new region without rust thus over long distances.Therefore, in the world, many soybeans they grow regions cause damage ASR.The impact of pathogene on output is significant: some regional observation to arrive most 80% production loss (YorinoriJ.T. (2004) Ferrugem " asi á tica " dasojanoBrasil: econtrole.In:JuniorJ.N.LazzarottoJ.J. (eds.) Documentos247.Embrapa, Londrina, Brazil, 36).

For preventing and treating this disease, applications of fungicides or use resistance or tolerant varieties.

Applications of fungicides causes hostile environment problem or the high dose rate required for effective controlling disease and the toxicological effect caused usually.

Know and known antifungal activity natural existence (Burdon, J.J. in the genotype of Glycine; Marshall, D.R. (1981) EvaluationofAustraliannativespeciesofGlycinecanescens, awildrelativeofsoybean.TheoreticalAppliedGenetics, 65:44-45; Burdon, J.J. (1988) MajorgeneresistancetoPhakopsorapachyrhiziinGlycinecanesc ens, awildrelativeofsoybean.TheoreticalAppliedGenetics, 75:923-928).Five main Qualitative resistant gene (MeyerJ.D.F. are identified, etal., (2009) Identificationandanalysesofcandidategenesforrpp4-mediate dresistancetoASRinsoybean, PlantPhysiology, 150:295-307; OlokaH.K.; TukamuhabwaP. (2008) ReactionofexoticsoybeangermplasmtoPhakopsorapachyrhiziin Uganda, PlantDisease, 92:1493-1496): rpp1 (McLean, R.J. in PI200492; Byth, D.E. (1980) Inheritanceofresistancetorust (Phakopsorapachyrhizi) insoybean.AustralianJournalAgriculturalresearch, 31:951-956); Rpp2 (Bromfield, K.R. in PI230970; HartwigE.E. (1980) Resistancetosoybeanrustandmodeofinheritance.CropScience, 20:254-255); Rpp3 (BromfieldK.R. in PI462312; Melching, J.S. (1982) Sourcesofspecificresistancetosoybeanrust.Phytopathology, 72:706); Rpp4 (HartwigR.R. (1986) Identificationofafourthmajorgeneconferringtoresistanceto soybeanrust.CropScience, 26:1135-1136) in PI459025 and rpp5 (MeyerJ.D.F. (2009) IdentificationandanalysesofcandidategenesforRpp4-mediate dresistancetoAsianSoybeanRustinsoybean.PlantPhysiol150:2 95-307).Resistance expressed by each gene is limited to concrete pathogene kind and can overcomes because of host resistance and pathogene toxicity common evolutionary at short notice.Although there is the soybean varieties with outstanding speciality such as ASR tolerance, they are not have resistance completely but only tolerate described disease.

Therefore, crop to the tolerance of fungi and active many situations and in can not meet the needs of agricultural practice.Two aspects are concentrated on: more effective fungicide is used and given the qualification of gene and/or the modification of ASR tolerance/resistance to using the research in new resistance source.

WO2010/049405 teaches a kind of method improving plant health, and it is by using the alanine compounds that replaces containing heteroaromatic and agricultural active compound carries out as the bond of fungicide fluorine azoles bacterium acid amides (fluxapyroxad), biphenyl pyrrole bacterium amine (bixafen), Boscalid (boscalid), isopyrazam (isopyrazam) or pyrrole metsulfovax (penthiopyrad).Wherein, the improvement of plant health can not provide the instruction to control ASR.

In addition, US2010/0093715 has reported and benzamide type fungicide has been applied to the collaborative raising that genetically modified plants create plant health.Although it is referred to the method for control ASR, instruction is limited to the plant with transgenosis modification.Do not record the genetically modified plants of ASR tolerance.

WO2010/046380 describes the method by pest control and/or raising plant health with Carbox amide process cultivated plant.In this file directory or embodiment, the soybean varieties of indication is genetically modified plants.The soybean varieties used in the biological Examples of WO2010/046380 contains the transgenosis of conferring herbicide tolerance.Do not enlighten soybean varieties succinate dehydrogenase inhibitors class (SDHI) fungicide being applied to the stem canker resistance of ASR tolerance and/or the conventional breeding of frogeye leaf spot resistance in WO2010/046380 and produce synergistic effect by between the speciality and the fungicide used of described plant.Therefore, the enough instructions about preventing and treating ASR in the stem canker resistance and/or frogeye leaf spot resistant soybean kind of conventional breeding or genetically modified ASR tolerance are not provided.

Conventional breeding comprises following aspect than the general advantage of genetically modified plants:

In many cases, the speciality introduced by conventional breeding is more stable compared with the speciality introduced by transgenic method.For conventional breeding plant, there is the risk of less environmental pollution (such as being caused by Horizontal Gene Transfer).Especially, for conventional breeding plant, do not have possibility by genetically modified plants for selecting object and risk that the conventional antibiotics resistance gene introduced is given.And, also can produce powerful economical advantage, because do not need expensive and longer deregulated program at every country for conventional breeding plant.In addition, the time span needed for conventional breeding kind and cost much shorter compared with transformed variety is developed.In addition, usually higher in the acceptance of some national consumers.In addition, for conventional breeding plant, there is not useful plant performance due to the transgenosis occurred at random and be introduced into genome and multilated or the risk even eliminated.

Therefore, one object of the present invention is to provide a kind of method, and it can use fungicide to prevent and treat ASR in the stem canker resistance and/or frogeye leaf spot resistant soybean kind of the ASR tolerance of conventional breeding.

In addition, another object of the present invention is to provide a kind of method, it can use fungicide to prevent and treat ASR in the stem canker resistance and/or frogeye leaf spot resistant soybean kind of the ASR tolerance of conventional breeding, wherein there is the synergistic effect of plant speciality and fungicide.

Another object of the present invention is to provide the method can preventing and treating ASR, wherein when applications of fungicides, the stem canker resistance of the ASR tolerance of conventional breeding and/or the outstanding speciality of frogeye leaf spot resistance plant cause the synergistic effect of opposing ASR.

Another object of the present invention is to provide a kind of method, its ASR that fungicide can be used to prevent and treat in the stem canker resistance and/or frogeye leaf spot resistant soybean kind of the ASR tolerance of conventional breeding, wherein there is the synergistic effect of plant speciality and fungicide, and wherein use conventional breeding to there is extra advantage compared with genetically modified plants, described advantage is selected from higher new speciality stability, less risk of environmental pollution, without the danger of being polluted by antibiotics resistance gene, economical advantage, higher Consumer acceptance, and without useful plants because the transgenosis that occurs at random is introduced and multilated or the risk even eliminated.

Unexpectedly, have now found that, use SDHI fungicide in the stem canker resistance and/or frogeye leaf spot resistant soybean kind of the ASR tolerance of conventional breeding, treat ASR and show synergistic effect between described plant speciality and the fungicide used.

In the context of the present invention, collaborative referring to uses SDHI fungicide to exceed adding and effect the expection of soybean rest fungus in conjunction with the stem canker resistance of the ASR tolerance of conventional breeding and/or frogeye leaf spot resistant soybean kind, therefore extends the stem canker resistance of the sphere of action of SDHI fungicide and the ASR tolerance of conventional breeding and/or the action principle scope expressed by frogeye leaf spot resistant soybean kind.

This finds to include relative to the creative step of prior art unexpectedly.Such as above-outlined; not only be compared with the kind of conventional breeding with the difference of WO2010/046380; in WO2010/046380 (139-140 page), genetically engineered soybean kind is belonged to the biological Examples of soybean; and be the transgenosis conferring herbicide tolerance (glyphosate (glyphosate)) of the soybean varieties used in WO2010/046380, and the performance of the change of the conventional breeding kind of application claims protection belongs to ASR tolerance, stem canker resistance and/or frogeye leaf spot resistance.The not synergistic effect that will produce between the speciality and the fungicide used of described plant about preventing and treating ASR of expectability fungicide of the present invention process.

Especially, have now found that, at least one SDHI fungicide is applied to stem canker resistance and/or the frogeye leaf spot resistant soybean kind of the ASR tolerance of conventional breeding, when be applied to the stem canker resistance of ASR tolerance of conventional breeding and/or frogeye leaf spot resistant soybean kind, the position of this plant, plant propagation material or its growth place time, with with identical SDHI fungicide to compared with the producible control rate of wild-type plant, create the synergistic function of opposing soybean rest fungus.

Therefore, the present invention relates to a kind of method of preventing and treating the stem canker resistance of the ASR tolerance of conventional breeding and/or the ASR of frogeye leaf spot resistant soybean kind, comprise and SDHI fungicide is applied to described plant, plant propagation material or its growth place.

According to the present invention's preferred embodiment, SDHI fungicide is at least applied to the stem canker resistance of the ASR tolerance of conventional breeding and/or the position of frogeye leaf spot resistant soybean kind, the leaf of preferred conventional breeding plant, root, flower and/or stem, and seed.

SDHI fungicide

In the present invention's preferred embodiment, have now found that, at least one is selected from stem canker resistance and/or frogeye leaf spot resistant soybean kind that following SDHI fungicide is applied to the ASR tolerance of conventional breeding, when being applied to the stem canker of ASR tolerance and/or the frogeye leaf spot resistant soybean kind of conventional breeding, the position of this plant, when plant propagation material or its growth place, with with the identical SDHI fungicide being preferably selected from compound as defined above to compared with the producible control rate of wild-type plant, produce the synergistic function of opposing soybean rest fungus: penta benzene pyrrole bacterium amine (penflufen), isopyrazam, biphenyl pyrrole bacterium amine, ring benzene pyrrole bacterium amine (sedaxan), fluorine azoles bacterium acid amides, fluorine pyrrole bacterium acid amides (fluopyram), pyrrole metsulfovax, Boscalid, N-[1-(2, 4-dichlorophenyl)-1-methoxy propyl-2-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, N-[9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, N-[(1S, 4R)-9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide and N-[(1R, 4S)-9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide.

According to another preferred embodiment of the present invention, described at least one SDHI fungicide is selected from penta benzene pyrrole bacterium amine, isopyrazam, biphenyl pyrrole bacterium amine, ring benzene pyrrole bacterium amine and fluorine azoles bacterium acid amides.

Penta benzene pyrrole bacterium amine (fluoro-1, the 3-dimethyl-1H-pyrazole-4-carboxamide of chemical name: N-[2-(1,3-dimethylbutyl) phenyl]-5-) and can WO03/010149 be seen based on the preparation method of commercial compound.

Isopyrazam comprises 3-(difluoromethyl)-1-methyl-N-[(1RS, 4SR, 9RS)-1,2,3,4-tetrahydrochysene-9-isopropyl-Isosorbide-5-Nitrae-methanonaphthalene-5-base] two kinds of cis-isomers of pyrazole-4-carboxamide and 3-(difluoromethyl)-1-methyl-N-[(1RS, 4SR, 9SR)-1,2,3,4-tetrahydrochysene-9-isopropyl-Isosorbide-5-Nitrae-methanonaphthalene-5-base] mixture of two kinds of transisomers of pyrazole-4-carboxamide.Isopyrazam also comprises isopyrazam (trans epimerism racemic modification 1RS, 4SR, 9SR), isopyrazam (trans epimerism enantiomter 1R, 4S, 9S), isopyrazam (trans epimerism enantiomter 1S, 4R, 9R), isopyrazam (cis epimerism racemic modification 1RS, 4SR, 9RS), isopyrazam (cis epimerism enantiomter 1R, 4S, 9R) with isopyrazam (cis epimerism enantiomter 1S, 4R, 9S).Isopyrazam and see WO2004/035589 based on the preparation method of commercial compound.

Biphenyl pyrrole bacterium amine (chemical name: N-(3 ', 4 '-two chloro-5-fluoro-1,1 '-biphenyl-2-base)-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide) and see WO03/070705 based on the preparation method of commercial compound.

Ring benzene pyrrole bacterium amine comprises 2 '-[(1RS, 2RS)-1,1 '-two ring third-2-base] two kinds of cis-isomer and 2 '-[(1RS of-3-(difluoromethyl)-1-methylpyrazole-4-formailide, 2SR)-1,1 '-two ring third-2-base] mixture of two kinds of transisomers of-3-(difluoromethyl)-1-methylpyrazole-4-formailide.Ring benzene pyrrole bacterium amine and see WO03/074491, WO2006/015865 and WO2006/015866 based on the preparation method of commercial compound.

Fluorine azoles bacterium acid amides (chemical name: 3-(difluoromethyl)-1-methyl-N-(3 ', 4 ', 5 '-trifluoro-biphenyl-2-base)-1H-pyrazole-4-carboxamide) and see WO2005/123690 based on the preparation method of commercial compound.

Fluorine pyrrole bacterium acid amides (chemical name: N-{ [the chloro-5-of 3-(trifluoromethyl)-2-pyridine radicals] ethyl }-2,6-dichloro-benzamides) and see EP-A-1531673 and WO2004/016088 based on the preparation method of commercial compound.

Pyrrole metsulfovax (chemical name: (RS)-N-[2-(1,3-dimethylbutyl 9-3-thienyl]-1-methyl-3-(trifluoromethyl)-pyrazole-4-carboxamide) and see EP0737682 based on the preparation method of commercial compound.

Boscalid (the chloro-N-of chemical name: 2-(4 '-chlordiphenyl-2-base) vitamin PP) and see DE19531813 based on the preparation method of commercial compound.

N-[1-(2,4-dichlorophenyl)-1-methoxy propyl-2-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide is known in WO2010/000612.

N-[9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, N-[(1S, 4R)-9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, with N-[(1R, 4S)-9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide is known in WO2007/048556.

The above-mentioned SDHI fungicide mentioned can be used alone or is combined with other active components, other active components as:

(be known in this active component illustrated by its " common name " and be recorded in such as PesticideManual (" ThePesticideManual ", 14thEd., BritishCropProtectionCouncil2006) or can search on the internet (such as http:// www.alanwood.net/pesticides)).

Ergosterol biosynthesis inhibitor, such as 4-dodecyl-2,6-thebaine (aldimorph), oxygen ring azoles (azaconazole), Bitertanol (bitertanol), bromuconazole (bromuconazole), cyproconazole (cyproconazole), diclobutrazol (diclobutrazole), Difenoconazole (difenoconazole), olefin conversion (diniconazole), R-olefin conversion (diniconazole-M), dodemorph (dodemorph), dodemorph acetate (dodemorphacetate), epoxiconazole (epoxiconazole), etaconazole (etaconazole), Fenarimol (fenarimol), RH-7592 (fenbuconazole), fenhexamid (fenhexamid), fenpropidin (fenpropidin), butadiene morpholine (fenpropimorph), Fluquinconazole (fluquinconazole), flurprimidol (flurprimidol), Flusilazole (flusilazole), Flutriafol (flutriafol), furconazole (furconazole), furconazole_cis (furconazole-cis), own azoles alcohol (hexaconazole), imazalil (imazalil), IMAZALIL (imazalilsulfate), glyoxalin (imibenconazole), plant bacterium azoles (ipconazole), metconazole (metconazole), nitrile bacterium azoles (myclobutanil), Naftifine (naftifine), nuarimol (nuarimol), Evil imidazoles (oxpoconazole), paclobutrazol (paclobutrazol), pefurazoate (pefurazoate), penconazole (penconazole), disease spends spirit (piperalin) Prochloraz (prochloraz), propiconazole (propiconazole), prothioconazoles (prothioconazole), pyributicarb (pyributicarb), pyrifenox (pyrifenox), quinconazole, simeconazoles (simeconazole), volution bacterium amine (spiroxamine), Tebuconazole (tebuconazole), Terbinafine (terbinafine), tetraconazole (tetraconazole), triazolone (triadimefon), Triadimenol (triadimenol), tridemorph (tridemorph), fluorine bacterium azoles (triflumizole), triforine (triforine), triticonazole (triticonazole), uniconazole P (uniconazole), uniconazole P (uniconazole-p), alkene frost benzyl azoles (viniconazole), voriconazole (voriconazole), 1-(4-chlorphenyl)-2-(1H-1,2,4-triazol-1-yl) suberol, 1-(2,2-dimethyl-2,3-dihydro-1H-indenes-1-base)-1H-imidazole-5-carboxylic acid methyl ester, N '-{ 5-(difluoromethyl)-2-methyl-4-[3-(trimethyl silyl) propoxyl group] phenyl }-N-ethyl-N-methyl imino group formamide, N-ethyl-N-methyl-N '-{ 2-methyl-5-(trifluoromethyl)-4-[3-(trimethyl silyl) propoxyl group] phenyl } imino group formamide and O-[1-(4-methoxyphenoxy)-3,3-dimethyl butyrate-2-base] 1H-imidazoles-1-thiocarboxylic.

Act on the inhibitor of compound (complex) I or II of respiratory chain (respiratorychain), such as carboxin (carboxin), fluorine mepanipyrim (diflumetorim), fenfuram (fenfuram), flutolanil (flutolanil), furametpyr (furametpyr), seed dressing amine (furmecyclox), mebenil (mepronil), oxycarboxin (oxycarboxin), thifluzamide (thifluzamide), 1-methyl-N-[2-(1, 1, 2, 2-tetrafluoro ethyoxyl) phenyl]-3-(trifluoromethyl)-1H-pyrazole-4-carboxamide, 3-(difluoromethyl)-1-methyl-N-[2-(1, 1, 2, 2-tetrafluoro ethyoxyl) phenyl]-1H-pyrazole-4-carboxamide, 3-(difluoromethyl)-N-[the fluoro-2-(1 of 4-, 1, 2, 3, 3, 3-hexafluoro propoxyl group) phenyl]-1-methyl isophthalic acid H-pyrazole-4-carboxamide and salt thereof.

Act on the inhibitor of the Complex II I of respiratory chain, such as pungent azoles mepanipyrim (ametoctradin), amisulbrom (amisulbrom), Fluoxastrobin (azoxystrobin), cyazofamid (cyazofamid), dimoxystrobin (dimoxystrobin), Enestroburin (enestroburin), famoxadone (famoxadone), Fenamidone (fenamidone), fluoxastrobin (fluoxastrobin), kresoxim-methyl (kresoxim-methyl), SSF 126 (metominostrobin), orysastrobin (orysastrobin), ZEN 90160 (picoxystrobin), pyraclostrobin (pyraclostrobin), pyrametostrobin, azoles bacterium ester (pyraoxystrobin), azoles amine bacterium ester pyrrole bacterium benzene prestige (pyribencarb), oxime bacterium ester (trifloxystrobin), (2E)-2-(2-{ [6-(3-chloro-2-methyl phenoxy group)-5-FU-4-base] oxygen base } phenyl)-2-(methoxyimino)-N-methylacetamide, (2E)-2-(ethoxy imino)-N-methyl-2-(2-{ [({ (1E)-1-[3-(trifluoromethyl) phenyl] ethylidene } amino) oxygen base] methyl } phenyl) acetamide, (2E)-2-(methoxyimino)-N-methyl-2-{2-[(E)-({ 1-[3-(trifluoromethyl) phenyl] ethyoxyl } imino group) methyl] phenyl } acetamide, (2E)-2-{2-[({ [(1E)-1-(3-{ [the fluoro-2-phenylethyl of (E)-1-] oxygen base } phenyl) ethylidene] is amino } oxygen base) methyl] phenyl }-2-(methoxyimino)-N-methylacetamide, (2E)-2-{2-[({ [(2E, 3E)-4-(2,6-dichlorophenyl) fourth-3-alkene-2-subunit] is amino } oxygen base) methyl] phenyl }-2-(methoxyimino)-N-methylacetamide, the chloro-N-of 2-(1,1,3-trimethyl-2,3-dihydro-1H-indenes-4-base) pyridine-3-carboxamide, 5-methoxyl group-2-methyl-4-(2-{ [({ (1E)-1-[3-(trifluoromethyl) phenyl] ethylidene } is amino) oxygen base] methyl } phenyl)-2,4-dihydro-3H-1,2,4-triazole-3-ketone, (2E)-2-{2-[({ cyclopropyl [(4-methoxyphenyl) imino group] methyl } sulfonyl) methyl] phenyl }-3-methoxy propyl-2-olefin(e) acid methyl ester, N-(3-ethyl-3,5,5-trimethylcyclohexyl)-3-(Formylamino)-2-Hydroxylbenzamide, 2-{2-[(2,5-dimethyl phenoxy) methyl] phenyl }-2-methoxy N-methylacetamide, (2R)-2-{2-[(2,5-dimethyl phenoxy) methyl] phenyl }-2-methoxy N-methylacetamide and salt thereof.

Mitosis and cell division inhibitor (mitosisandcelldivisioninhibitor), such as benomyl (benomyl), carbendazim (carbendazim), benzene imidazoles bacterium (chlorfenazole), the mould prestige of second (diethofencarb), Han Lening (ethaboxam), fluopicolide (fluopicolide), furidazol (fuberidazole), Pencycuron (pencycuron), probenazole (thiabendazole), thiophanate-methyl (thiophanate-methyl), thiophanate (thiophanate), zoxamide (zoxamide) and the chloro-7-of 5-(4-methyl piperidine-1-base)-6-(2, 4, 6-trifluorophenyl) [1, 2, 4] triazole [1, 5-a] pyrimidine, the chloro-5-of 3-(6-chloro-pyridine-3-base)-6-methyl-4-(2, 4, 6-trifluorophenyl) pyridazine and salt thereof.

There is the compound that multidigit point (multisite) is active, such as bordeaux mixture mixture (Bordeauxmixture), difoltan (captafol), captan (captan), tpn (chlorothalonil), Kocide SD, copper naphthenate (coppernaphthenate), copper oxide, COPPER OXYCHLORIDE 37,5 (copperoxychloride), copper sulphate (+2), dichlofluanid (dichlofluanid), dithianon (dithianon), dodine (dodine), dodine free alkali (dodinefreebase), ferbam (ferbam), fluorine folpet (fluorofolpet), folpet (folpet), the pungent salt of biguanides (guazatine), guazatine acetate (guazatineacetate), iminoctadine (iminoctadine), biguanides octyl benzene sulfonate (iminoctadinealbesilate), iminoctadine triacetate (iminoctadinetriacetate), mancopper (mancopper), mancozeb (mancozeb), maneb (maneb), Carbatene (metiram), Carbatene zinc (metiramzinc), copper 8-hydroxyquinolinate (oxine-copper), Brolene (propamidine), Propineb (propineb), sulphur and sulphur preparation are as calcium polysulfide, tmtd (thiram), Tolylfluanid (tolylfluanid), zineb (zineb), ziram (ziram), 2,6-dimethyl-1H, 5H-[Isosorbide-5-Nitrae] dithiene [2,3-c:5,6-c '] two pyrroles-1,3,5,7 (2H, 6H)-tetraketone and salt thereof.

The compound of host defense can be induced, such as diazosulfide (acibenzolar-S-methyl), isotianil (isotianil), probenazole (probenazole), tiadinil (tiadinil) and salt thereof.

Amino acid and protein biosynthesis inhibitor, such as amine puts out (andoprim), blasticidin-S (blasticidin-S), cyprodinil (cyprodinil), kasugarnycin (kasugamycin), kasugarnycin one hydrochloride hydrate (kasugamycinhydrochloridehydrate), mepanipyrim (mepanipyrim), phonetic mould amine (pyrimethanil) and salt thereof.

ATP produces inhibitor, such as fentin acetate (fentinacetate), triphenyl tin chloride (fentinchloride), fentin hydroxide (fentinhydroxide) and Silthiopham (silthiofam).

Cell wall synthesis inhibitor, such as benzene metsulfovax (benthiavalicarb), dimethomorph (dimethomorph), flumorph (flumorph), iprovalicarb (iprovalicarb), mandipropamid (mandipropamid), polyoxin (polyoxins), Polyoxin (polyoxorim), Validacin (Takeda) (validamycinA) and phonetic aniline sulphur grand (valifenalate).

Lipid (lipid) and film synthetic inhibitor, such as biphenyl, chloroneb (chloroneb), botran (dicloran), edifenphos (edifenphos), soil bacterium goes out (etridiazole), methylamine acid esters (iodocarb), iprobenfos (iprobenfos), Isoprothiolane (isoprothiolane), Propamocarb (propamocarb), propamocarb (propamocarbhydrochloride), flutter bacterium sulphur (prothiocarb), pyrazophos (pyrazophos), pcnb (quintozene), tecnazene (tecnazene) and tolelofos-methyl (tolclofos-methyl).

Melanin biosynthesis inhibitor, such as ring propionyl bacterium amine (carpropamid), two chlorine zarilamid (diclocymet), zarilamid (fenoxanil), Rabcide (phthalide), pyroquilon (pyroquilon) and tricyclazole (tricyclazole).

Nucleic acid synthetic inhibitor (nucleicacidsynthesisinhibitor), such as M 9834 (benalaxyl), essence M 9834 (benalaxyl-M (kiralaxyl)), bupirimate (bupirimate), clozylacon, dimethirimol (dimethirimol), the phonetic phenol of second (ethirimol), furalaxyl (furalaxyl), hymexazo (hymexazol), metalaxyl (metalaxyl), mefenoxam (metalaxyl-M (mefenoxam)), ofurace (ofurace), the spirit of Evil frost (oxadixyl) and oxolinic acide (oxolinicacid).

Signal transduction inhibitor, such as chlozolinate (chlozolinate), fenpiclonil (fenpiclonil), fludioxonil (fludioxonil), iprodione (iprodione), procymidone (procymidone), benzene oxygen quinoline (quinoxyfen) and vinclozolin (vinclozolin)

Can as the compound separating even agent (uncoupler), such as binapacryl (binapacryl), karathane (dinocap), ferimzone (ferimzone), fluazinam (fluazinam) and the mite that disappears many (meptyldinocap).

Other compounds, such as benthiozole (benthiazole), bethoxazin, capsimycin (capsimycin), carvol (carvone), chinomethionat (chinomethionat), chlazafenone, cufraneb (cufraneb), cyflufenamid (cyflufenamid), frost urea cyanogen (cymoxanil), cyprosulfamide, dazomet (dazomet), debacarb (debacarb), antiphen (dichlorophen), diclomezin (diclomezine), difenzoquat (difenzoquat), difenzoquat methylsulfuric acid ester (difenzoquatmethylsulphate), diphenylamines (diphenylamine), ecomate, fenpyrazamine, fluorine acyl bacterium amine (flumetover), fluoromide (fluoroimide), flusulfamide (flusulfamide), flutianil, phosethyl-Al (fosetyl-Auminium), triethylphosphine acid calcium (fosetyl-calcium), triethylphosphine acid sodium (fosetyl-sodium), hexachloro-benzene (hexachlorobenzene), people's metamycin (irumamycin), methasulfocarb (methasulfocarb), methyl-isorhodanate (methylisothiocyanate), metrafenone (metrafenone), midolthromycin (mildiomycin), myprozine (natamycin), Sankel (nickeldimethyldithiocarbamate), nitrothalisopropyl (nitrothal-isopropyl), octhilinone (octhilinone), oxamocarb, oxyfenthiin, pentachlorophenol (pentachlorophenol) and salt thereof, phenothrin (phenothrin), phosphoric acid and salt thereof, propamocarb-fosetylate, propanosine-sodium, third oxygen quinoline (proquinazid), pyrrolnitrine, tebufloquin, tecloftalam, tolnifanide, triazoxide (triazoxide), fungi spirit (trichlamide), zarilamid (zarilamid), 1-(4-{4-[(5R)-5-(2, 6-difluorophenyl)-4, 5-dihydro-1,2-oxazole-3-base]-1,3-thiazoles-2-base } piperidin-1-yl)-2-[5-methyl-3-(trifluoromethyl)-1H-pyrazol-1-yl] ethyl ketone, 1-(4-{4-[(5S)-5-(2, 6-difluorophenyl)-4, 5-dihydro-1,2-oxazole-3-base]-1,3-thiazoles-2-base } piperidin-1-yl)-2-[5-methyl-3-(trifluoromethyl)-1H-pyrazol-1-yl] ethyl ketone, 1-(4-{4-[5-(2,6-difluorophenyl)-4,5-dihydro-1,2-oxazole-3-base]-1,3-thiazoles-2-base } piperidin-1-yl)-2-[5-methyl-3-(trifluoromethyl)-1H-pyrazol-1-yl] ethyl ketone, 1-(4-methoxyphenoxy)-3,3-dimethyl butyrate-2-base 1H-imidazoles-1-carboxylate, the chloro-4-of 2,3,5,6-tetra-(methyl sulphonyl) pyridine, 2,3-dibutyl-6-chloro thiophene also [2,3-d] pyrimidine-4 (3H)-one, 2-[5-methyl-3-(trifluoromethyl)-1H-pyrazol-1-yl]-1-(4-{4-[(5R)-5-phenyl-4,5-dihydro-1,2-oxazole-3-base]-1,3-thiazoles-2-base } piperidin-1-yl) ethyl ketone, 2-[5-methyl-3-(trifluoromethyl)-1H-pyrazol-1-yl]-1-(4-{4-[(5S)-5-phenyl-4,5-dihydro-1,2-oxazole-3-base]-1,3-thiazoles-2-base } piperidin-1-yl) ethyl ketone, 2-[5-methyl-3-(trifluoromethyl)-1H-pyrazol-1-yl]-1-{4-[4-(5-phenyl-4,5-dihydro-1,2-oxazole-3-base)-1,3-thiazoles-2-base] piperidin-1-yl } ethyl ketone, the iodo-3-propyl group of 2-butoxy-6--4H-chromene-4-ketone, the chloro-5-of 2-[the chloro-1-of 2-(the fluoro-4-methoxyphenyl of 2,6-bis-)-4-methyl isophthalic acid H-imidazoles-5-base] pyridine, 2-phenylphenol and salt, 3,4,5-trichloropyridine-2,6-dintrile, 3-[5-(4-chlorophenyl)-2,3-dimethyl-1,2-oxazole-3-base] pyridine, the chloro-5-of 3-(4-chlorphenyl)-4-(2,6-difluorophenyl)-6-methyl pyridazine, 4-(4-chlorphenyl)-5-(2,6-difluorophenyl)-3,6-dimethyl pyridazine, 5-amido-1,3,4-thiadiazoles-2-mercaptan, the chloro-N ' of 5--phenyl-N '-(the third-2-alkynes-1-base) thiophene-2-sulfo group hydrazides, 5-methyl-6-octyl group [1,2,4] triazole [1,5-a] pyrimidine-7-amine, (2Z)-3-amino-2-cyano group-3-Cinnamic Acid ethyl ester, N-(4-chlorphenylmethyl)-3-[3-methoxyl group-4-(the third-2-alkynes-1-base oxygen base) phenyl] propionamide, N-[(4-chlorophenyl) (cyano group) methyl]-3-[3-methoxyl group-4-(the third-2-alkynes-1-base oxygen base) phenyl] propionamide, N-[(the bromo-3-chloro-pyridine of 5--2-base) methyl]-2,4-dichloropyridine-3-formamides, N-[1-(the bromo-3-chloro-pyridine of 5--2-base) ethyl]-2,4-dichloropyridine-3-formamides, N-[1-(5-bromo-3-chloro-pyridine-2-base) ethyl] the fluoro-4-iodine pyridine of-2--3-formamide, N-{ (E)-[(cyclo propyl methoxy) imino group] [6-(difluoro-methoxy)-2,3-difluorophenyl] methyl }-2-phenyl-acetamides, N-{ (Z)-[(cyclo propyl methoxy) imino group] [6-(difluoro-methoxy)-2,3-difluorophenyl] methyl }-2-phenyl-acetamides, N-methyl-2-(1-{ [5-methyl-3-(trifluoromethyl)-1H-pyrazol-1-yl] acetyl group } piperidin-4-yl)-N-(1,2,3,4-naphthane-1-base)-1,3-thiazoles-4-formamide, N-methyl-2-(1-{ [5-methyl-3-(trifluoromethyl)-1H-pyrazol-1-yl] acetyl group } piperidin-4-yl)-N-[(1R)-1,2,3,4-naphthane-1-base]-1,3-thiazoles-4-formamide, N-methyl-2-(1-{ [5-methyl-3-(trifluoromethyl)-1H-pyrazol-1-yl] acetyl group } piperidin-4-yl)-N-[(1S)-1,2,3,4-naphthane-1-base]-1,3-thiazoles e-4-formamide, { 6-[({ [(1-methyl isophthalic acid H-tetrazolium-5-base) (phenyl) methylene] is amino } oxygen base) methyl] pyridine-2-base } carbamic acid amyl group ester, phenazine-1-carboxylic acid, quinoline-8-alcohol and quinoline-8-alcohol sulphonic acid ester (2: 1).

Other compounds are 1-methyl-3-(trifluoromethyl)-N-[2 '-(trifluoromethyl) biphenyl-2-base]-1H-pyrazole-4-carboxamide such as, N-(4 '-askarel-2-base)-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, N-(2 ', 4 '-DCBP-2-base)-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, 3-(difluoromethyl)-1-methyl-N-[4 '-(trifluoromethyl) biphenyl-2-base]-1H-pyrazole-4-carboxamide, N-(2 ', 5 '-DfBP-2-base)-1-methyl-3-(trifluoromethyl)-1H-pyrazole-4-carboxamide, 3-(difluoromethyl)-1-methyl-N-[4 '-(the third-1-alkynes-1-base) biphenyl-2-base]-1H-pyrazole-4-carboxamide, 5-fluoro-1, 3-dimethyl-N-[4 '-(the third-1-alkynes-1-base) biphenyl-2-base]-1H-pyrazole-4-carboxamide, the chloro-N-of 2-[4 '-(the third-1-alkynes-1-base) biphenyl-2-base] pyridine-3-carboxamide, 3-(difluoromethyl)-N-[4 '-(3, 3-dimethyl butyrate-1-alkynes-1-base) biphenyl-2-base]-1-methyl isophthalic acid H-pyrazole-4-carboxamide, N-[4 '-(3, 3-dimethyl butyrate-1-alkynes-1-base) biphenyl-2-base]-5-fluoro-1, 3-dimethyl-1H-pyrazole-4-carboxamide, 3-(difluoromethyl)-N-(4 '-acetenyl biphenyl-2-base)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, N-(4 '-acetenyl biphenyl-2-base)-5-fluoro-1, 3-dimethyl-1H-pyrazole-4-carboxamide, the chloro-N-of 2-(4 '-acetenyl biphenyl-2-base) pyridine-3-carboxamide, the chloro-N-of 2-[4 '-(3, 3-dimethyl butyrate-1-alkynes-1-base) biphenyl-2-base] pyridine-3-carboxamide, 4-(difluoromethyl)-2-methyl-N-[4 '-(trifluoromethyl) biphenyl-2-base]-1, 3-thiazole-5-formamide, the fluoro-N-of 5-[4 '-(3-hydroxy-3-methyl fourth-1-alkynes-1-base) biphenyl-2-base]-1, 3-dimethyl-1H-pyrazole-4-carboxamide, the chloro-N-of 2-[4 '-(3-hydroxy-3-methyl fourth-1-alkynes-1-base) biphenyl-2-base] pyridine-3-carboxamide, 3-(difluoromethyl)-N-[4 '-(3-methoxyl group-3-methyl fourth-1-alkynes-1-base) biphenyl-2-base]-1-methyl isophthalic acid H-pyrazole-4-carboxamide, the fluoro-N-of 5-[4 '-(3-methoxyl group-3-methyl fourth-1-alkynes-1-base) biphenyl-2-base]-1, 3-dimethyl-1H-pyrazole-4-carboxamide, the chloro-N-of 2-[4 '-(3-methoxyl group-3-methyl fourth-1-alkynes-1-base) biphenyl-2-base] pyridine-3-carboxamide, (the bromo-2-methoxyl group of 5--4-picoline-3-base) (2, 3, 4-trimethoxy-6-aminomethyl phenyl) and ketone and N-[2-(4-{ [3-(4-chlorphenyl) third-2-alkynes-1-base] oxygen base }-3-methoxyphenyl) ethyl]-N2-(methyl sulphonyl) valine amide.

Acetylcholinesterase (AChE) inhibitor, such as carbamates, such as alanycarb (alanycarb), Aldicarb (aldicarb), Evil worm prestige (bendiocarb), Benfuracard micro (benfuracarb), butocarboxim (butocarboxim), butanone sulfone prestige (butoxycarboxim), carbaryl (carbaryl), carbofuran (carbofuran), carbosulfan (carbosulfan), ethiofencarb (ethiofencarb), Bassa (fenobucarb), Carzol (formetanate), furathiocarb (furathiocarb), Mobucin (isoprocarb), methiocarb (methiocarb), Methomyl (methomyl), meta-tolyl-N-methylcarbamate (MTMC) (metolcarb), oxamyl (oxamyl), Aphox (pirimicarb), unden (propoxur), thiodicarb (thiodicarb), thiofanox (thiofanox), triaguron (Triazamate), Landrin (trimethacarb), XMC (XMC) and Meobal (xylylcarb), or organophosphorus compounds such as orthene (acephate), Jia Ji Bi Evil phosphorus (azamethiphos), azinphos ethyl (azinphos-ethyl), methyl azinphos-methyl (azinphos-methyl), cadusafos (cadusafos), chlorethoxyfos (chlorethoxyfos), chlorfenviphos (chlorfenvinphos), chlormephos (chlormephos), chlopyrifos (chlorpyrifos), chlorpyrifos-methyl (chlorpyrifos-methyl), Resistox (coumaphos), cynock (cyanophos), demeton-methyl (demeton-S-methyl), diazinon (diazinon), dichlorvos/DDVP (dichlorvos/DDVP), Carbicron (dicrotophos), Rogor (dimethoate), dimethylvinphos (dimethylvinphos), disulfoton (disulfoton), EPN, ethion (ethion), phonamiphos (ethoprophos), famphur (famphur), fenamiphos (fenamiphos), sumithion (fenitrothion), fenthion (fenthion), lythidathion (fosthiazate), heptenophos (heptenophos), imicyafos, chlorine isofenphos (isofenphos), O-isopropyl salicylate (IsopropylO-(methoxyaminothio-phosphoryl) salicylate), oxazole phosphorus (isoxathion), malathion (malathion), Afos (mecarbam), acephatemet (methamidophos), methidathion (methidathion), Menite (mevinphos), nuvacron (monocrotophos), 2-dichloroethylk dimethyl phosphate (naled), omethoate (omethoate), oxydemeton_methyl (oxydemeton-methyl), parathion (parathion), parathion-methyl (parathion-methyl), phenthoate dimephenthoate cidial (phenthoate), thimet (phorate), Phosalone (phosalone), phosmet (phosmet), phosphamidon (phosphamidon), phoxim (phoxim), pirimiphos-methyl (pirimiphos-methyl), Profenofos (profenofos), propetamphos (propetamphos), Toyodan (prothiofos), pyraclofos (pyraclofos), pyridaphethione (pyridaphenthion), quinalphos (quinalphos), sulfotep (sulfotep), butyl pyrimidine phosphorus (tebupirimfos), Swebate (temephos), terbufos (terbufos), Ravap (tetrachlorvinphos), thiometon (thiometon), Hostathion (triazophos), chlorophos (triclorfon) and vamidothion (vamidothion).

The controlled chloride channel antagonists of GABA door, such as cyclic diolefine organochlorine class, such as Niran (chlordane) and 5a,6,9,9a-hexahydro-6,9-methano-2,4 (endosulfan); Or phenyl pyrazoles (phenylpyrazoles (fiproles)), such as second worm nitrile (ethiprole) and ethiprole (fipronil).

Sodium channel modulators/potential dependent sodium channel blockers, such as pyrethroid, such as fluorine ester chrysanthemum ester (acrinathrin), allethrin (Allethrin), the cis-anti-allethrin (d-cis-transallethrin) of d-, the anti-allethrin of d-(d-transallethrin), bifenthrin (bifenthrin), bioallethrin (bioallethrin), bioallethrin-S-cyclopenta isomer (bioallethrin-S-cyclopentenylisomer), Chryson (Bioresmethrin), cycloprothrin (cycloprothrin), cyfloxylate (cyfluthrin), β-cyfloxylate (beta-Cyfluthrin), Cyhalothrin (cyhalothrin), λ-three cyhalothrin (lambda-cyhalothrin), ζ-three cyhalothrin (gamma-cyhalothrin), cypermethrin (cypermethrin), α-cypermethrin (alpha-cypermethrin), β-cypermethrin (beta-cypermethrin), θ-cypermethrin (theta-cypermethrin), ζ-cypermethrin (zeta-cypermethrin) phenylate permethrin (cyphenothrin) [(1R)-transisomer], decis (deltamethrin), Prallethrin (1R isomer) (empenthrin ((EZ)-(1R) isomer)), esfenvalerate (esfenvalerate), ether chrysanthemum ester (etofenprox), fenpropathrin (fenpropathrin), kill chrysanthemum ester (fenvalerate), flucythrinate (flucythrinate), flumethrin (flumethrin), τ-taufluvalinate (tau-fluvalinate), halfenprox (Halfenprox), Imiprothrin (imiprothrin), kadethrin (kadethrin), permethrin (Permethrin), phenothrin (1R-transisomer) (phenothrin (1R-trans-isomer), prallethrin (prallethrin), pyrethrins (pyrethrine (pyrethrum)), Chryson (Resmethrin), silafluofene (silafluofen), tefluthrin (tefluthrin), tetramethrin (teramethrin), tetramethrin (1R isomer) (tetramethrin (1Risomer)), tralomethrin (tralomethrin) and transfluthrin (transfluthrin), or DDT, or methoxychlor.

Acetylcholinergic receptor (nAChR) activator, such as nicotinoids, such as pyrrole worm clear (acetamiprid), clothianidin (clothianidin), MTI-446 (dinotefuran), Imidacloprid (imidacloprid), Nitenpyram (nitenpyram), thiacloprid (thiacloprid) and Diacloden (thiamethoxam); Or nicotine.

Acetylcholinergic receptor (nAChR) allosteric activation agent such as multiple killing teichomycin class (spinosyns), such as spinetoram and pleocidin (spinosad).

Chloride channel activators class, such as Avermectin (avermectins)/milbemycin (milbemycins), such as Avermectin (abamectin), emamectin-benzoate (emamectinbenzoate), lepimectin and milbemectin (milbemectin).

Juvenile hormone mimics (mimics) class, such as juvenile hormone homologue, such as hydroprene (hydroprene), kinoprene (kinoprene) and methoprene (methoprene) or fenoxycarb (Fenoxycarb); Or Nylar (pyriproxyfen).

Various indefinite (multidigit point) inhibitor, such as alkyl halide, such as methyl bromide and other alkyl halides; Or chloropicrin (chloropicrin); Or sulfuryl fluoride (Sulfurylfluoride) or sodium tetraborate (borax); Or tartar emetic (Tartaremetic).

Selectivity Homoptera feeding deterrent class, such as pymetrozine (pymetrozine); Or flonicamid (flonicamid).

Mite growth inhibitor, such as clofentezine (clofentezine), Hexythiazox (hexythiazox) and fluorine mite piperazine (Diflovidazin); Or second mite azoles (Etoxazole).

Insect gut membrane micro agent interfering class, such as Bacillus thuringiensis subsp. israelensis (Bacillusthuringiensissubspeciesisraelensis), Bacillus sphaericus (Bacillussphaericus), bacillus thuringiensis (Bacillusthuringiensissubspeciesaizawai), B. thuringiensis subspecies (Bacillusthuringiensissubspecieskurstaki), bacillus thuringiensis intends walking first subspecies (Bacillusthuringiensissubspeciestenebrionis) and BT phytoprotein: as Cry1Ab, Cry1Ac, Cry1Fa, Cry2Ab, mCry3A, Cry3Ab, Cry3Bb, Cry34/35Ab1.

Mitochondrial ATP synthase inhibitor, such as diafenthiuron (diafenthiuron); Or organotin acaricide, such as azacyclotin (azocyclotin), plictran (cyhexatin) and fenbutatin oxide (fenbutatin-oxide); Or propargite (propargite); Or tetradifon (tetradifon).

The oxidative phosphorylation uncoupler class worked by being interrupted proton gradient, such as capillary (chlorfenapyr), DNOC and sulfluramid (sulfluramid).

Acetylcholinergic receptor (nAChR) channel blocker, such as bensultap (bensultap), cartap (Cartaphydrochloride), thiocyclam (thiocyclam) and dimehypo (thiosultap-sodium).

Benzoylurea derivertives class, 0 type, such as bistrifluron (bistrifluron), UC 62644 (chlorfluazuron), TH-6040 (diflubenzuron), flucycloxuron (flucycloxuron), flufenoxuron (flufenoxuron), HEXAFLUMURON (hexaflumuron), fluorine third oxygen urea (lufenuron), Rimon (novaluron), noviflumuron (noviflumuron), volt worm phosphorus (teflubenzuron) and desinsection grand (triflumuron).

Benzoylurea derivertives class, 1 type, such as Buprofezin (buprofezin).

To cast off a skin agent interfering class, such as cyromazine (cyromazine).

Ecdysone receptor activator class, such as ring worm hydrazides (chromafenozide), chlorine worm hydrazides (halofenozide), methoxyfenozide (methoxyfenozide) and worm hydrazides (tebufenozide).

Octopamine energy receptor stimulating agent class, such as Amitraz (amitraz).

Mitochondria complex compound III electron transfer inhibitors, such as amdro (hydramethylnon); Or acequinocyl (acequinocyl); Or fluacrypyrim (fluacrypyrim).

Mitochondria complex compound I electron transfer inhibitors, such as METI miticide, such as fenazaquin (fenazaquin), fenpyroximate (fenpyroximate), pyrimidifen (pyrimidifen), pyridaben (pyridaben), tebufenpyrad (tebufenpyrad) and Tolfenpyrad (tolfenpyrad); Or rotenone (rotenone (Derris)).

Voltage gated sodium channels blocking agent class, such as indoxacarb (indoxacarb); Or metaflumizone (metaflumizone).

Acetyl group CoA carboxylase inhibitor class; such as tetronic acid derivatives and tetramic acid derivatives, such as spiral shell mite ester (spirodiclofen), Spiromesifen (spiromesifen) and spiral shell worm ethyl ester (spirotetramat).

Mitochondria complex compound IV electron transfer inhibitors, such as phosphine class, such as aluminum phosphate, calcium phosphide, phosphine and zinc phosphide; Or cyanide.

Point stereocomplex II electron transfer inhibitors, such as nitrile pyrrole mite ester (cyenopyrafen).

Ryanodine (ryanodine) receptor modulators class, such as diamines, such as chlorantraniliprole (Chlorantraniliprole) and Flubendiamide (flubendiamide).

Are unknown or uncertain mechanism of other active compounds, such as amidoflumet, neem element (azadirachtin), benclothiaz, benzene mite (benzoximate), diphenyl hydrazine ester (bifenazate), manganese bromine mite ester (bromopropylate), eliminate mites (chinomethionat), cryolite, cryolite, bromine cyanide insect amide (Cyantraniliprole (Cyazypyr)), cyflumetofen, dicofol (dicofol), Diflovidazin, Fluensulfone, its insect amine (flufenerim), butyl nitrile (Flufiprole) bacteria, fluorine pyrazole amide, Fufenozide, consult moiety (Imidaclothiz), Iprodione, insect propyl ether (Pyridalyl), quinazoline (Pyrifluquinazon) and methyl iodide (iodomethane);Based on Bacillusfirmus (I - 1582, BioNeem, Votivo) of other products or one of the following compounds: 3 - bromine - N - (2 - bromine - 4 - chlorine - 6 - [(1 - ethyl) cyclopropyl amino formyl] phenyl} - 1 - (3 - chlorinated pyridine - 2 -) 1 h - pyrazole - 5 - formamide (known in WO2005/077934), 4 - {[(6 - pyridine bromide generation - 3 -) methyl] amino} (2 - fluoro ethyl) furan - 2 (5 h) - ketone (known in WO2007/115644),4 - {[(6 - fluoro pyridine - 3 -) methyl] amino} (2, 2 - ethyl 2 fluorine) furan - 2 (5 h) - ketone (known in WO2007/115644), 4 - {[(2 - chloro - 1, 3 - thiazole - 5 -) methyl] amino} (2 - fluoro ethyl) furan - 2 (5 h) - ketone (known in WO2007/115644), 4 - {[(6 - chlorinated pyridine - 3 -) methyl] amino} (2 - fluoro ethyl) furan - 2 (5 h) - ketone (known in WO2007/115644), 4 - {[(6 - chlorinated pyridine - 3 -) methyl] amino} (2, 2 - ethyl 2 fluorine) furan - 2 (5 h) - ketone (known in WO2007/115644), 4 - {[(6 - chloro - 5 - fluoro pyridine - 3 -) methyl] (methyl) amine} furan - 2 (5 h) - ketone (known in WO2007/115643), 4 - {[(5, 6 - dichloro generation of pyridine - 3 -) methyl] amino} (2 - fluoro ethyl) furan - 2 (5 h) - ketone (known in WO2007/115646), 4 - {[(6 - chloro - 5 - fluoro pyridine - 3 -) methyl] (cyclopropyl amino} furan - 2 (5 h) - ketone (known in WO2007/115643), 4 - {[(6 - chloro pyridine - 3 - base)Methyl] (cyclopropyl amino} furan - 2 (5 h) - ketone (known in the EP - A - 0539588), 4 - {[(6 - chlorinated pyridine - 3 -) methyl] (methyl) amine} furan - 2 (5 h) - ketone (known in the EP - A - 0539588), {[1 - (6 - chlorinated pyridine - 3 -) ethyl] 4 - sulfur ketone (methyl) oxygen - lambda} cyanamide (known in WO2007/149134) and the diastereomer {[(1 R) 1 - (6 - chlorinated pyridine - 3 -) ethyl] 4 - sulfur ketone (methyl) oxygen - lambda} cyanamide (A) and {[(1 S) - 1 - (6 - chlorinated pyridine - 3 -) ethyl] 4 - sulfur ketone (methyl) oxygen - lambda} cyanamide (B) (also known in WO2007/149134), and fluorine organism worm amine nitrile (sulfoxaflor) (also known in WO2007/149134) and the diastereomer [(R) - methyl (oxygen) {(1 R) - 1 - [6 - (3 methyl) pyridine - 3 -] ethyl} 4 - sulphur - lambda ketone] cyanamide (A1) and [(S) - methyl (oxygen) {(1 S) - 1 - [6 - (3 methyl) pyridine - 3 -] ethyl} 4 - sulphur - lambda ketone] cyanamide (A2), called diastereomer class A (known in WO2010 /074747, WO2010/074751), [(R) - methyl (oxygen) {(1 S) - 1 - [6 - (3 methyl) pyridine - 3 -] ethyl} 4 - sulphur - lambda ketone] cyanamide (B1) and [(S) - methyl (oxygen) {(1 R) - 1 - [6 - (3 methyl) pyridine - 3 -] ethyl} 4 - sulphur - lambda ketone] cyanamide (B2), known as the diastereomer class B (also known in WO2010/074747, WO2010/074751) and 11 - (4 - chloro - 2, 6 - dimethyl phenyl) - 12 - hydroxy - 1, 4-2 oxygen mixed - 9 - nitrogen impurity two screw [4.2.4.2] four decyl - 11 - ene - 10 - ketone (known in WO2006/089633), 3 - (4 '- fluoro - 2, 4 - dimethyl biphenyl - 3 - base) - 4 - hydroxy - 8 - oxygen mixed - 1 - nitrogen impurity screw [4.5] decyl - 3 - ene - 2 - ketone (known in WO2008/067911), 1 - {4 - methyl - 2 - fluoro - 5 - [(2,2,2 - trifluoro ethyl) of sulfonyl] phenyl} - 3 - (three fluorinated methyl) - 1 h - 1, 4-trichlorobenzene triazole - 5 - amine (known in WO2006/043635), [(12 R, 3 S, 4 ar, 12 as, bs) - 3 - [(cyclopropyl carbonyl oxygen] 2 hydroxy - 4-6, 12 -,- dimethyl - 11-12 b oxygen generation - 9 -,3,4,4 (pyridine - 3 -) - 1 a, 5,6,6 a, 12, 12 a, 12 b - dihydro - 2 h, 11 h - benzo [f] pyran [4, 3 - b] color olefin - 4 - methyl cyclopropane carboxylic acid ester base] (known in WO2008/066153), 2 cyano - 3 - (2 methoxy) - N, N - dimethyl benzene sulfonamide (known in WO2006/056433), 2 cyano - 3 - (2 methoxy) - N - methyl benzene sulfonamide (known in WO2006/100288), 2 cyano - 3 - (2 methoxy) - N - ethyl benzene sulfonamide (known in WO2005/035486), 4 - (2 methoxy) - N - ethyl - N - methyl - 1, 2 - benzo thiazole - 1, 1-3 - amine oxides (known in WO2007/057407), N - [1 - (2, 3 - dimethyl phenyl) - 2 - (3, 5 - dimethyl phenyl) ethyl] - 4, 5 - dihydro - 1, 3 - thiazole - 2 - amine (known in WO2008/104503) and {1 '- [(e) 2-3 - (4 - chlorobenzene) c - 2 - ene - 1 - base] - 5 - fluorine screw [indole - 3, 4' - piperidine] - 1 (2 h) -} (2 - chlorinated pyridine - 4 - base)Ketone (known in WO2003/106457), 3 - (2, 5 - dimethyl phenyl) - 4 - hydroxy - 8 - methoxy - 1, 8 - dinitrogen miscellaneous screw [4.5] decyl - 3 - ene - 2 - ketone (known in WO2009/049851), 3 - (2, 5 - dimethyl phenyl) - 8 - methoxy - 2 - oxygen generation 1, 8 - dinitrogen miscellaneous screw [4.5] decyl - 3 - ene - 4 - ethyl carbonate (known in WO2009/049851), 4 - (2 - acetylene - 1 - butyl - oxygen radicals) - 6 - (3, 5 - dimethyl piperidine - 1 - base) - 5 - fluorine pyrimidine (known in WO2004/099160), (2,2,3,3,4,4,5,5 - amyl) eight fluorine filling (3 - trifluoro propyl) malononitrile (known in WO2005/063094), (2,2,3,3,4,4,5,5 - eight fluorine amyl) (3,3,4,4,4 - butyl five fluorine) malononitrile (known in WO2005/063094), 8 - [2 - (cyclopropyl methoxy) - 4 - (3 methyl) phenoxy] - 3-6 - [(3 methyl) pyridine - 3 -]- 3 - nitrogen impurity bicyclic [3.2.1] octane (known in WO2007/040280), 2 - ethyl - 7 - methoxy - 3 - methyl - 6 - [(2,2,3,3 - four fluorine - 2, 3-2-1, 4 - hydrogen oxygen benzene and 2-6 - base) oxygen] quinoline - 4 - base methyl carbonate (known in JP2008/110953), 2 - ethyl - 7 - methoxy - 3 - methyl - 6 - [(2,2,3,3 - four fluorine - 2, 3-2 h - 1, 4 - benzene and 2 4-dioxane - 6 - base) oxygen radicals] quinoline - 4 - base acetate (known in JP2008/110953), PF1364 (CAS - Reg. No. 1204776-60-2) (known in JP2010/018586), 5 - [5 - (3, 5 - dichlorobenzene) - 5 - (three fluorinated methyl) - 4, 5 - dihydro - 1, 2 - oxazole - 3 - base] - 2 - (1 h - 1, 4-trichlorobenzene triazole - 1 - base) benzonitrile (known in WO2007/075459), 5 - [5 - (2 - chlorinated pyridine - 4 - base) - 5 - (three fluorinated methyl) - 4, 5 - dihydro - 1, 2 - oxazole - 3 - base] - 2 - (1 h - 1, 4-trichlorobenzene triazole - 1 - base) benzonitrile (known in WO2007/075459), 4 - [5 - (3, 5 - dichlorobenzene) - 5 - (three fluorinated methyl) - 4, 5 - dihydro - 1, 2 - oxazole - 3 - base]- 2 - methyl - N - (2 - oxygen generation - 2 - [(2,2,2 - three fluorinated ethyl) amino] ethyl} benzamide (known in WO2005/085216), 4 - {[(6 - chlorinated pyridine - 3 -) methyl] (cyclopropyl amino}) - 1, 3 - oxazole - 2 (5 h) - ketone, 4 - {[(6 - chlorinated pyridine - 3 -) methyl] amino} (2, 2 - ethyl 2 fluorine) - 1, 3 - oxazole - 2 (5 h) - ketone, 4 - {[(6 - chlorinated pyridine - 3 -) methyl] amino} (ethyl) - 1, 3 - oxazole - 2 (5 h) - ketone, 4 - {[(6 - chlorinated pyridine - 3 -) methyl] amino} (methyl) - 1, 3 - oxazole - 2 (5 h) - ketone (all known in WO2010/005692), NNI - 0711 (known in WO2002096882), 1 - acetyl - N - [4 - (1,1,1,3,3,3 - six fluorine - 2 - methoxy - 2 - c) - 3 - isobutyl phenyl] - N - butyl acyl - 3, 5 - dimethyl - 1 h - pyrazole - 4 - formamide (known in WO2002096882), methyl 2 - (2 - ({[3 - bromine - 1 - (3 - chlorinated pyridine - 2 -) 1 h - pyrazole - 5 - base] carbonyl} amino) - 5 - chloro - 3 - methyl benzoic acyl]- 2 - methyl hydrazine carboxylic acid ester (known in WO2005/085216), methyl 2 - (2 - ({[3 - bromine - 1 - (3 - chlorinated pyridine - 2 -) 1 h - pyrazole - 5 - base] carbonyl} amino) - 5 - cyano - 3 - methyl benzoic acyl] - 2 - ethyl hydrazine carboxylic acid ester (known in WO2005/085216), methyl 2 - (2 - ({[3 - bromine - 1 - (3 - chlorinated pyridine - 2 -) 1 h - pyrazole - 5 - base] carbonyl} amino) - 5 - cyano - 3 - methyl benzoic acyl] - 2 - methyl hydrazine carboxylic acid ester (known in WO2005/085216), methyl 2 - (3, 5 - two br - 2 - ({[3 - bromine - 1 - (3 - chlorinated pyridine - 2 -) 1 h - pyrazole - 5 - base] carbonyl} amino) benzoic acyl] - 1, 2 - diethyl hydrazine carboxylic acid ester (known in WO2005/085216), methyl 2 - (3, 5 - two br - 2 - ({[3 - bromine - 1 - (3 - chlorinated pyridine - 2 -) 1 h - pyrazole - 5 - base] carbonyl} amino) benzoic acyl] - 2 - ethyl hydrazine carboxylic acid ester (known in WO2005/085216), (rs 5, 7 rs; rs 5, 7 sr) - 1 - (6 - chloro - 3 - pyridyl methyl) - 1,2,3,5,6,7 - six fluorine - 7 - methyl - 8 - XiaoJi imidazole and - 5 - c oxygen radicals[1, 2 - a] pyridine (known in WO2007/101369), 2 - (6 - [2 - (5 - fluoro pyridine - 3 - base) - 1, 3 - thiazole - 5 - base] pyridine - 2 - base} pyrimidine (known in WO2010/006713), 2 - (6 - [2 - (pyridine - 3 -) - 1, 3 - thiazole - 5 - base] pyridine - 2 - base} pyrimidine (known in WO2010/006713), 1 - (3 - chlorinated pyridine - 2 - base) - N - (4 - cyano - 2 - methyl - 6 - (methyl amino formyl) phenyl] - 3 - {[5 - (three fluorinated methyl) - 1 - base] 1 h - tetrazolium - methyl} - 1 h - pyrazole - 5 - formamide (known in WO2010/069502), 1 - (3 - chlorinated pyridine - 2 - base) - N - (4 - cyano - 2 - methyl - 6 - (methyl amino formyl) phenyl] - 3 - {[5 - (three fluorinated methyl) - 2 h - tetrazolium - 2 - base] methyl} - 1 h - pyrazole - 5 - formamide (known in WO2010/069502), N - [2 - (butylated amino formyl) - 4 - cyano - 6 - methyl phenyl]- 1 - (3 - chloro pyridine - 2 - base) - 3 - {[5 - (three fluorinated methyl) - 1 - base] 1 h - tetrazolium - methyl} - 1 h - pyrazole - 5 - formamide (known in WO2010/069502), N - [2 - (butylated amino formyl) - 4 - cyano - 6 - methyl phenyl] - 1 - (3 - chlorinated pyridine - 2 - base) - 3 - {[5 - (three fluorinated methyl) - 2 h - tetrazolium - 2 - base] methyl} - 1 h - pyrazole - 5 - formamide (known in WO2010/069502) and (e) 1 - N - [(6 - chlorinated pyridine - 3 -) methyl] - N '- cyano - N - (2, 2 - ethyl 2 fluorine) acetamidine (known in WO2008/009360).

The stem canker resistance of the ASR tolerance of conventional breeding and/or frogeye leaf spot resistant soybean kind

The stem canker resistance of the ASR tolerance of conventional breeding and/or frogeye leaf spot resistant soybean kind and plant parts thereof can according to process of the present invention.Plant parts is interpreted as referring to all grounds of plant and position, underground and organ, and as bud, leaf, Hua Hegen, the example that can mention is leaf, stem, flower, sporocarp and seed, and root welding technology.Plant parts also comprises crop material and asexual and case of propagation material, such as cutting, rhizome, tiller and seed.Preferably, plant parts is interpreted as referring to the leaf of conventional breeding plant, root, flower and/or stem, and seed.More preferably, plant parts is interpreted as referring to leaf.

The plant that the present invention uses or plant variety have ASR tolerance, stem canker resistance and/or frogeye leaf spot resistance.Preferably, the ASR tolerance of plant of the present invention or plant variety is by being selected from the gene of Rpp1, Rpp2, Rpp3, Rpp4 and Rpp5 or it combines and gives.More preferably, ASR tolerance is by being selected from the gene of Rpp2, Rpp4 and Rpp5 or it combines and gives.

The plant that the present invention uses or plant variety are not genetically modified.Foreign gene (transgenosis) is introduced into organism alive by using gene engineering method and produces by transgenic organism, makes organism to show the performance made new advances.The genetic material of genetically modified plants not easily passs through the recombinant DNA technology that crossbreeding, sudden change or natural restructuring obtain under field conditions (factors) modify by using, ASR tolerance, stem canker resistance and/or frogeye leaf spot resistance are given in this modification thus, or give the ASR tolerance, stem canker resistance and/or the frogeye leaf spot resistance that improve.

Gene engineering uses gene transformation technology to the genomic direct labor's operation of organism.Use transformation technology is inserted into the DNA sequence dna in genome, and no matter it is from different plant species or from same species, is all referred to as " transgenosis " at this." transgenosis " also contains the antisense sequences that can cause gene silencing, or synonym and antisense sequences.

Numerous methods of Plant Transformation are developed, comprise biological and physics Plant Transformation scheme (see such as Glick, B.R.andThompson, J.E.Eds. (CRCPress, Inc., BocaRaton (1993): 67-88andArmstrong, " TheFirstDecadeofMaizeTransformation:AReviewandFuturePers pective " Maydica1999 (44): 101-109).In addition, the expression vector of plant cell or metaplasia and plant regeneration and extracorporeal culture method are obtainable.See such as Glick, B.R.andThompson, J.E.Eds. (CRCPress, Inc., BocaRaton (1993): 89-119).

On the contrary, non-transgenic plant is produced by traditional breeding technology (such as hybridize/backcross/selfing etc.).

Preferably, the stem canker resistance of the ASR tolerance of conventional breeding of the present invention and/or frogeye leaf spot resistant soybean kind are obtained by segregative generation (segregatinggeneration), preferred bulk method (bulkMethod), SSD (single seed descent) and/or backcross method.

More preferably, the stem canker resistance of the ASR tolerance of conventional breeding of the present invention and/or frogeye leaf spot resistant soybean kind are obtained by bulk method.In bulk method, segregative generation (usual F2 and F5) with all plants mixed in population sowing and gather and grow.Therefore, in bulk method, for the seed that the grows each segregative generation sample of seed for gathering in a first generation.After oneself adds fertile crop five generation, plant performance goes out the homozygosis of height and can select for (the Souza that gathers separately, A.P.BiologiaMolecularAplicadaaomelhoramento.In:RecursosG en é ticoseMelhoramento-Plantas.LucianoL.Nass; AlfonsoC.C.Valois, ItamarS.deMelo; MariaCl é liaValadres-Inglis. (Org.) 1 ^aed.Ronon ó polis, 2001,1:939-966).

In another embodiment, the stem canker resistance of the ASR tolerance of conventional breeding of the present invention and/or frogeye leaf spot resistant soybean kind are obtained by SSD method.SSD method is recorded in (Brim, C.A. in 1966 by Brim; 1966.Amodifiedpedigreemethodofselectioninsoybeans.CropSc ience, 6:20), and be separated by (from F2 to F5) the generation single basin (2 to 3 seeds) of gathering from every strain plant improved and form; But, only there is a strain plant in every basin for growing the next generation.Gather and preserve sample.Thus, last in method, every strain corresponds to different F2 plants, thus reduces the loss caused by sampling defect or natural selection.

In another preferred embodiment, the stem canker resistance of the ASR tolerance of conventional breeding of the present invention and/or frogeye leaf spot resistant soybean kind are obtained by backcross method.Exactly, backcross method is not a kind of method growing isolated species.It is a kind of phenotypic expression for improvement of defect speciality, the strategy especially when this speciality has qualitative inheritance.Its use can make to come from the gene of the parent being referred to as supplier parent (DP) or some gene transfer are referred to as the parent of backcross parent (RP) to another, and this backcross parent is generally has certain cultivated species receiving paying close attention in market needing the defect improved in its cultivation.This defect can by revising defective for the not tool gene transfer from supplier parent to the method for backcross parent.This process, namely from the individuality of isolated species and the hybridization of backcross parent, be called and backcross, and again can obtain the reproduction parent genotype (Souza of almost 100%, A.P.BiologiaMolecularAplicadaaomelhoramento.In:RecursosG en é ticoseMelhoramento-Plantas.LucianoL.Nass; AlfonsoC.C.Valois, ItamarS.deMelo; MariaCl é liaValadres-Inglis. (Org.) 1 ^aed.Ronon ó polis, 2001,1:939-966).

Preferably, last at process for selective as above, differentiates to cause one or some pure lines with outstanding speciality of new cultivated species.

Most preferably, the plant variety that the present invention uses is TropicalMelhoramentoeGen é ticaLTDA, TMG801 and/or TMG803 of Brasil.

Wild-type soy is preferably a Plants, it does not have the outstanding speciality of stem canker resistance as the ASR tolerance of conventional breeding and/or frogeye leaf spot resistant soybean kind, but all similar as much as possible to the soybean used in the inventive method in any other characteristic.The genome of wild-type plant, transcript profile, Leaf proteins or metabolism group are similar as much as possible to the plant used in the inventive method.Preferably, Wild-type soy is Miyagishirome kind.

With SDHI fungicide of the present invention to the stem canker resistance of the ASR tolerance of conventional breeding and/or frogeye leaf spot resistant soybean kind, its plant parts, the process that plant propagation material or its growth place are carried out directly is carried out, or by using conventional treatment method to act on environment, habitat or storage area and carry out, described conventional treatment method is such as by dipping, spraying, atomizing, mist process, evaporation, dust, atomization, dispersion, foaming, spraying, broadcast sowing, injection, drenched, drip irrigation, and at propagating materials, particularly when seed, can also by dry kind of facture, wet kind of facture, slurries facture, pass through involucrum, by being coated with one or more layers dressing etc.In addition, also SDHI fungicide is used by super-low capacity area method.Preferably, SDHI fungicide is sprayed to soybean varieties, plant parts or its growth place.

The present invention also provides the preparation and administration form prepared by the Fungicidal composition comprising at least one SDHI fungicide of the present invention as above.Described fungicide formulations or administration form preferably comprise those of auxiliary agent (as extender, solvent and carrier) and/or other auxiliary agents (as surface reactive material).

The example of conventional formulation comprises solution, emulsion agent, wetting powder, water base and oil-based suspension, water base and Oil-based Suspension concentrating agents, pulvis, efflorescence product, paste, soluble powder, granule, dispersible granule, soluble granule, broadcast sowing with granule, suspo-emulsion concentrating agents, with the natural material of reactive compound dipping, synthetic material with reactive compound dipping, fertilizer and the Ultrafine Capsules agent in polymerizable compound.Preferably, the administration form prepared by SDHI fungicide is emulsion.

These preparations are prepared in a conventional manner, such as by by reactive compound and auxiliary agent as extender, solvent and/or solid carrier, and/or other auxiliary agents such as surface reactive material is mixed with.Preparation is in suitable equipment or prepare before administration or in application.

The auxiliary agent used can be that the preparation can giving SDHI fungicide or the administration form prepared by these preparations (such as crop production compositions namely, as spray liquid or seed coat agent) are with the material of specific performance (as some physics, technology and/or biological property).

The example of suitable extender comprises water, polarity and nonpolar organic chemistry liquid, such as be selected from aromatics and non-aromatic hydrocarbons (as paraffin, alkylbenzene, Fluhyzon, chlorobenzene), alcohol and polyalcohol (if need to be substituted, etherificate and/or esterification), ketone (as acetone, cyclohexanone), ester (comprising fat and oil) and (gathering) ether, simply with the amine replaced, acid amides, lactam (as N-alkyl pyrrolidone) and lactone, sulfone and sulfoxide (as dimethyl sulfoxide (DMSO)).

When using water as extender, organic solvent also can be used as cosolvent.The liquid flux considered is following material substantially: aromatic compounds, as dimethylbenzene, toluene or Fluhyzon; The aromatic compounds of chlorination or the aliphatic hydrocarbon of chlorination, as chlorobenzene, vinyl chloride or carrene; Aliphatic hydrocarbon, as cyclohexane; Or paraffin, as petroleum distillate; Mineral and vegetable oil; Alcohol, as butanols or ethylene glycol, and ether and ester; Ketone, as acetone, MEK, methyl iso-butyl ketone (MIBK) or cyclohexanone; Intensive polar solvent is as dimethyl formamide and dimethyl sulfoxide (DMSO); And water.

All suitable solvents can be used in principle.The example of suitable solvent is aromatic hydrocarbon, as dimethylbenzene, toluene or Fluhyzon; The aromatic hydrocarbon of chlorination or the aliphatic hydrocarbon of chlorination, as chlorobenzene, vinyl chloride or carrene; Aliphatic hydrocarbon, as cyclohexane; Paraffin, petroleum distillate; Mineral oil and vegetable oil; Alcohol, as methyl alcohol, ethanol, isopropyl alcohol, butanols or ethylene glycol, and ether and ester; Ketone, as acetone, MEK, methyl iso-butyl ketone (MIBK) or cyclohexanone; Intensive polar solvent, as dimethyl sulfoxide (DMSO); And water.Preferably, use acetone as solvent.

All suitable carriers can be used in principle.The carrier considered is more particularly following material: such as, the mineral that ammonium salt and natural fine are pulverized, as kaolin, alumina, talcum, chalk, quartz, attapulgite, montmorillonite or diatomite, and the mineral that the fine powder of synthesis is broken, as silica, the aluminium oxide of high degree of dispersion, and natural or that synthesize silicate, resin, wax and/or solid fertilizer.The mixture of this kind of carrier can be used equally.Consider that the carrier being used for granule comprises following material: such as, pulverize and the natural minerals of classification, as calcite, marble, float stone, sepiolite, dolomite, and the synthesis particle of the broken organic and inorganic substances of fine powder, and organic material is as the particle of sawdust, ester, coconut husk, cornstalk and cigarette stalk.

Gaseous state extender or the solvent of liquefaction can also be used.Suitable be more particularly in normal temperature and be those extenders or the carrier of gaseous state at atmosheric pressure, example is aerosol propellants, as halogenated hydrocarbons, and butane, propane, nitrogen and carbonic acid gas.

Surface reactive material for the object of the invention is emulsifier and/or foam agent, it is ionic to have or the dispersant of nonionic or wetting agent, or the mixture of these surface reactive materials.The example is polyacrylic salt, the salt of lignosulphonic acid, the salt of phenol sulfonic acid or naphthalene sulfonic acids, oxirane and fatty alcohol or with fatty acid or the condensation polymer with fatty acid amide, with the condensation polymer of substituted phenol (preferred alkyl phenol or aryl phenol), the salt of sulfosuccinate, taurine derivatives (preferred alkyl taurate), the alcohol of polyethoxylated or the phosphate of phenol, the fatty acid ester of polyalcohol, and comprise sulphate, the derivative of sulfonate and phosphatic compound, such as alkylaryl polyglycol ether, alkylsulfonate, alkyl sulfate, arylsulphonate, protein hydrolysate, lignin-sulfite waste liquor and methylcellulose.And if if water insoluble being applied in water of one of one of SDHI fungicide and/or inert carrier is carried out, then the existence of surfactant is favourable.Preferably, use polyoxyethylene alkyl phenyl ether as emulsifier.

Other auxiliary agents existed in the administration form that preparation neutralization is obtained by them can comprising toner, as inorganic pigment, example is iron oxide, titanium oxide and Prussian blue, and organic dyestuff, as alizarin dyes, azo dyes and metallized phthalocyanine dye, and nutrient, comprise trace nutrient, as molysite, magnesium salts, boron salt, mantoquita, cobalt salt, molybdenum salt and zinc salt.

What exist in addition can be that stabilizing agent is as low-temperature stabilization agent, preservative, antioxidant, light stabilizer or other reagent strengthening chemistry and/or physical stability.What can exist in addition is foam agent or defoamer.

The preparation obtained by it and administration form can also comprise following material as other auxiliary agent: sticker, as carboxymethyl cellulose; Natural and the synthetic polymer of powder, particle or latex form, as gum Arabic, polyvinyl alcohol, polyvinyl acetate; And natural phospholipid, as cephalin and lecithin; And synthetic phospholipid.Other possible auxiliary agents comprise mineral oil and vegetable oil.

If need, preparation and by they obtain administration form in can also there are other auxiliary agents.The example of this kind of adjuvant comprises aromatic, protecting colloid, adhesive, adhesive, thickener, thixotropic agent, bleeding agent, maintenance promoter, stabilizing agent, chelating agent and complexing agent.Generally speaking, reactive compound can be combined with any solid or liquid adjuvant being generally used for preparation object.

SDHI fungicide can with its commercially available conventional formulations, also can with the administration form prepared by those preparations, exist with the mixture of other reactive compounds, other reactive compounds described are as insecticide, attractant, apholate, bactericide, miticide, nematocide, fungicide, growth regulator, weed killer herbicide, safener, fertilizer, infochemical or other reagent strengthening plant performance.

Preparation preferably contains the SDHI fungicide of 0.00000001 % by weight to 98 % by weight, or particularly preferably 0.01 % by weight to 95 % by weight SDHI fungicide, more preferably 0.5 % by weight to 90 % by weight, the most preferably SDHI fungicide of 1 % by weight, based on the weighing scale of preparation.In above-mentioned context, term " SDHI fungicide " also comprises the bond of SDHI fungicide.

Preferably, preparation comprises at least one SDHI fungicide, solvent, emulsifier and/or water.

The SDHI fungicide content of the administration form (crop production compositions) prepared by preparation can change in wide scope.The SDHI fungicide concentration of administration form can be the SDHI fungicide of 0.002ppm to 500ppm, preferred 0.2ppm to 500ppm, more preferably 0.2ppm to 100ppm usually.In above-mentioned context, term " SDHI fungicide " also comprises the bond of SDHI fungicide.

Most preferably, penta benzene pyrrole bacterium amine is used as the emulsion of the described fungicide containing 0.2ppm to 50ppm.

Most preferably, isopyrazam is used as the emulsion of the described fungicide containing 0.2ppm to 1ppm.

Most preferably, biphenyl pyrrole bacterium amine is used as the emulsion of the described fungicide containing 0.2ppm to 100ppm.

Most preferably, ring benzene pyrrole bacterium amine is used as the emulsion of the described fungicide containing 0.2ppm to 25ppm.

Most preferably, fluorine azoles bacterium acid amides is used as the emulsion of the described fungicide containing 0.2ppm to 25ppm.

The use capacity of SDHI fungicide to the soybean varieties of conventional breeding, plant propagation material or its growth place is 0.01kg/ha to 3kg/ha, preferred 0.01kg/ha to 1.5kg/ha, more preferably 0.02kg/ha to 0.5kg/ha.Preferably, the capacity of using of SDHI fungicide is 25-500l/ha, preferred 25-250l/ha.

Preferably, the present invention relates to the stem canker resistance of the ASR tolerance comprising conventional breeding and/or the Kit of parts (akitofparts) of frogeye leaf spot resistant soybean kind and SDHI fungicide, described SDHI fungicide is selected from penta benzene pyrrole bacterium amine, isopyrazam, biphenyl pyrrole bacterium amine, ring benzene pyrrole bacterium amine, fluorine azoles bacterium acid amides, fluorine pyrrole bacterium acid amides, pyrrole metsulfovax, Boscalid, N-[1-(2, 4-dichlorophenyl)-1-methoxy propyl-2-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, N-[9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, N-[(1S, 4R)-9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide and N-[(1R, 4S)-9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide.

According to another embodiment of the present invention, Kit of parts comprises the stem canker resistance of the ASR tolerance of conventional breeding and/or frogeye leaf spot resistant soybean kind and SDHI fungicide, and described SDHI fungicide is selected from penta benzene pyrrole bacterium amine, isopyrazam, biphenyl pyrrole bacterium amine, ring benzene pyrrole bacterium amine and fluorine azoles bacterium acid amides.According to another embodiment of the present invention, Kit of parts comprises stem canker resistance and/or the frogeye leaf spot resistant soybean kind TMG801 and/or TMG803 of the ASR tolerance of conventional breeding.

Other preferred embodiments about Kit of parts comprise embodiment as defined above.

The present invention also comprises the method for producing agricultural products, comprise stem canker resistance and/or frogeye leaf spot resistant soybean kind, its plant propagation material of ASR tolerance SDHI fungicide being applied to conventional breeding, or its growth place, and produce agricultural products by the position of described plant or this plant or plant propagation material.

According to the present invention's preferred embodiment, the SDHI fungicide used in the method for described production agricultural products is selected from penta benzene pyrrole bacterium amine, isopyrazam, biphenyl pyrrole bacterium amine, ring benzene pyrrole bacterium amine, fluorine azoles bacterium acid amides, fluorine pyrrole bacterium acid amides, pyrrole metsulfovax, Boscalid, N-[1-(2, 4-dichlorophenyl)-1-methoxy propyl-2-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, N-[9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, N-[(1S, 4R)-9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide and N-[(1R, 4S)-9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide.

According to another preferred embodiment of the present invention, the SDHI fungicide used in the method for described production agricultural products is at least applied to the stem canker resistance of the ASR tolerance of conventional breeding and/or the position of frogeye leaf spot resistant soybean kind, the leaf of preferred conventional breeding plant, root, flower and/or stem, and seed.According to another preferred embodiment, SDHI fungicide is used as emulsion, the described fungicide preferably containing 0.2 to 500ppm.According to another preferred embodiment, the capacity of using of SDHI is 0.01 to 3kg/ha, preferably 0.01 to 1.5kg/ha, more preferably 0.02 to 0.5kg/ha.According to another preferred embodiment, the ASR tolerance in the stem canker resistance of the ASR tolerance of conventional breeding and/or frogeye leaf spot resistant soybean kind is by being selected from the gene of Rpp1, Rpp2, Rpp3, Rpp4 and Rpp5 or it combines and gives.Even more preferably, ASR tolerance is by being selected from the gene of Rpp2, Rpp4 and Rpp5 or it combines and gives.Also preferably, the stem canker resistance of the ASR tolerance of conventional breeding and/or frogeye leaf spot resistant soybean kind are TMG801 and/or TMG803.

The another preferred embodiment of the present invention, also for SDHI fungicide is for the purposes of the ASR of the stem canker resistance and/or frogeye leaf spot resistant soybean kind of preventing and treating the ASR tolerance of conventional breeding, comprises and SDHI fungicide is applied to described plant, plant propagation material or its growth place.

Embodiment

Cultivate in greenhouse until 1.5 leaf phases by a sensitive varieties (Miyagishirome) with to the soybean varieties (TMG801 and TMG803) that soybean rest fungus (Phakosporapachyrhizi) has a tolerance.By using the SDHI composition of 1 weight portion with the acetone of 28.5 weight portions as solvent with mix as the polyoxyethylene alkyl phenyl ether of 1.5 weight portions of emulsifier, the appropriate formulation of preparation SDHI fungicide.With water, concentrate is diluted to required concentration.

The rice shoot SDHI fungicide formulations of sensitive varieties and tolerant soybean kind is sprayed under described rate of application, to assess the activity of control soybean rest fungus.

Spraying one day after, inoculates bean plant with the aqueous spore suspension of the cause of disease reagent (causalagent) of soybean rest fungus.Then plant is placed in about 20 DEG C and relative atmospheric humidity be about 80% greenhouse.Process is tested below implementing:

11 days assessment plants after inoculation.The percentage of the leaf area be contaminted is determined in range estimation.According to ABBOTT result of calculation (% drug effect).0% refers to the drug effect being equivalent to control group, and the drug effect of 100% refers to and do not observe disease.

Determine the expection drug effect of SDHI fungicide and ASR tolerant varieties bond according to Colby formula and contrast (Colby with observation drug effect, S.R. (1967) Calculatingsynergisticandantagonisticresponsesofherbicid ecombinations, Weeds, 15:20-22):

E=x+y-xy／100

Wherein E is the expection drug effect when using the bond of fungicide and ASR tolerant varieties, represents to check percentage; X is the drug effect of the percentage inspection when using fungicide; Y is the drug effect of the percentage inspection when using ASR tolerant varieties.

By contrast expection drug effect and observation drug effect, synergistic effect can be discerned.If higher than the expection percentage drug effect calculated, then there is synergistic effect in the percentage drug effect of observation.

table 1. penta benzene pyrrole bacterium amine

table 2. isopyrazam

table 3. biphenyl pyrrole bacterium amine

table 4. ring benzene pyrrole bacterium amine

table 5. fluorine azoles bacterium acid amides

Result clearly shows, the bond of SDHI fungicide and soybean rest fungus tolerant varieties provides the synergistic effect to preventing and treating soybean rest fungus.The SDHI fungicide of the present invention observed and the activity of tolerant varieties bond are greater than the activity using Colby formulae discovery.

The most significant synergistic effect is found when the fluorine azoles bacterium acid amides of 1ppm active component being applied to TMG803.Described fungicide effect is at low concentrations than the more remarkable effect be up under 25ppm activity component concentration.The synergistic effect of same trend is found in the ring benzene pyrrole bacterium amine being applied to TMG803.This fungicide is used to provide and under the concentration of 5ppm active component to 25ppm active component, is used higher synergy than ring benzene pyrrole bacterium amine under low concentration (0.2ppm active component is to 1ppm active component).

By comparison, biphenyl pyrrole bacterium amine is applied to TMG803 and shows the highest synergistic effect under the concentration of 100ppm active component to 25ppm active component.

When application concentration is penta benzene pyrrole bacterium amine of 10ppm active component, obtain the highest deviation of actual drug effect and expection drug effect.This and vegetation type (TMG801 or TMG803) have nothing to do.

Generally speaking, TMG803 shows and has nothing to do with the SDHI fungicide used to the higher potential of agreement.

Claims (15)

1. prevent and treat the method for the stem canker resistance of Asian Soybean rest fungus (ASR) tolerance of conventional breeding and/or the ASR of frogeye leaf spot resistant soybean kind for one kind, comprise succinate dehydrogenase inhibitors class (SDHI) fungicide is applied to described soybean varieties plant, plant propagation material or its growth place, wherein the stem canker resistance of the ASR tolerance of conventional breeding and/or frogeye leaf spot resistant soybean kind are TMG801 or TMG803.

2. the method for claim 1, wherein SDHI fungicide is selected from penta benzene pyrrole bacterium amine, isopyrazam, biphenyl pyrrole bacterium amine, ring benzene pyrrole bacterium amine, fluorine azoles bacterium acid amides, fluorine pyrrole bacterium acid amides, pyrrole metsulfovax, Boscalid, N-[1-(2, 4-dichlorophenyl)-1-methoxy propyl-2-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, N-[9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, N-[(1S, 4R)-9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide and N-[(1R, 4S)-9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide.

3. the method for claim 1 or 2, wherein SDHI fungicide is at least applied to the stem canker resistance of the ASR tolerance of conventional breeding and/or the position of frogeye leaf spot resistant soybean kind.

4. the method for claim 3, wherein SDHI fungicide is at least applied to the leaf of the plant of described conventional breeding soybean varieties, root, flower and/or stem, and seed.

5. the method for claim 1 or 2, wherein ASR tolerance is given by the gene or its combination being selected from Rpp1, Rpp2, Rpp3, Rpp4 and Rpp5.

6. the method for claim 1 or 2, wherein ASR tolerance is given by the gene or its combination being selected from Rpp2, Rpp4 and Rpp5.

7. the method for claim 1 or 2, wherein SDHI fungicide is used as emulsion.

8. the method for claim 7, wherein said emulsion contains the described fungicide of 0.2 to 500ppm.

9. the method for claim 1 or 2, wherein the capacity of using of SDHI fungicide is 0.01 to 3kg/ha.

10. the method for claim 1 or 2, wherein the capacity of using of SDHI fungicide is 0.01 to 1.5kg/ha.

The method of 11. claims 1 or 2, wherein the capacity of using of SDHI fungicide is 0.02 to 0.5kg/ha.

12. 1 kinds of Kit of parts, comprise the stem canker resistance of the ASR tolerance of conventional breeding and/or frogeye leaf spot resistant soybean kind and SDHI fungicide, described SDHI fungicide is selected from penta benzene pyrrole bacterium amine, isopyrazam, biphenyl pyrrole bacterium amine, ring benzene pyrrole bacterium amine or fluorine azoles bacterium acid amides, fluorine pyrrole bacterium acid amides, pyrrole metsulfovax, Boscalid, N-[1-(2, 4-dichlorophenyl)-1-methoxy propyl-2-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, N-[9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, N-[(1S, 4R)-9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide and N-[(1R, 4S)-9-(dichloromethylene)-1, 2, 3, 4-tetrahydrochysene-1, 4-methanonaphthalene-5-base]-3-(difluoromethyl)-1-methyl isophthalic acid H-pyrazole-4-carboxamide, wherein the stem canker resistance of the ASR tolerance of conventional breeding and/or frogeye leaf spot resistant soybean kind are TMG801 and/or TMG803.

13. 1 kinds of methods of producing agricultural products, comprise stem canker resistance and/or frogeye leaf spot resistant soybean kind, its plant propagation material or its growth place of ASR tolerance SDHI fungicide being applied to conventional breeding, and produce agricultural products by the position of described plant or this plant or plant propagation material, wherein the stem canker resistance of the ASR tolerance of conventional breeding and/or frogeye leaf spot resistant soybean kind any one of claim 1,5 and 6 limit.

The method of 14. claims 13, wherein SDHI fungicide as claim 2 limit.

The method of 15. claims 13 or 14, wherein SDHI fungicide any one of claim 3 and 7 to 9 limit and use.