CN103445263A - Zymolytic ovalbumin compound nutritional oral solution - Google Patents
Zymolytic ovalbumin compound nutritional oral solution Download PDFInfo
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- CN103445263A CN103445263A CN2013104231161A CN201310423116A CN103445263A CN 103445263 A CN103445263 A CN 103445263A CN 2013104231161 A CN2013104231161 A CN 2013104231161A CN 201310423116 A CN201310423116 A CN 201310423116A CN 103445263 A CN103445263 A CN 103445263A
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Abstract
The invention provides a zymolytic ovalbumin compound nutritional oral solution. A modern high technology is used for carrying out biochemical enzymolysis on egg white to obtain a micro-molecule polypeptide with very strong biological activity, namely zymolytic ovalbumin. The polypeptide has different functions on a human body due to different composition structures and the functions are concluded into inhibition, activation, repairing and acceleration. The cytopathic effect is inhibited, the immunity of a human body is boosted, the activity of cells is activated, harmful free radicals to the human body are eliminated, diseased cells of the human body are repaired, the metabolism is improved, and the synthesis and the regulation and controlling of proteins, enzymes and ferments of the human body are accelerated. The zymolytic ovalbumin polypeptide is used as a bioactive peptide, has the physiological functions of resisting mutation, preventing radiation, resisting fatigues, protecting the liver, resisting oxidization, reducing blood fat, reducing blood pressure and the like, and also has huge development and application values in the fields of foods, cosmetics, medical treatment, chemical engineering and the like.
Description
[technical field]
This product design health food technology field, specifically a kind of enzymolysis ovalbumin compound nutrition oral administration.
[background technology]
Utilize modern high technology that egg white is carried out to biochemical enzymolysis, obtaining having the very micromolecule polypeptide of strong biological activity is exactly enzyme-hydrolysis albumen.Polypeptide, because the difference that forms structure has different functions to human body, sums up as suppressing, activate, repair and promoting.Suppress cytopathy, strengthen body immunity; The active cell activity, remove harmful free radical; Repair the cell of pathology of human body, improve metabolism; Promote the synthetic and regulation and control of human body protein, enzyme and ferment.The enzyme-hydrolysis albumen polypeptide is as a kind of biologically active peptide; there is anti-sudden change, radiation proof, antifatigue, protection liver, anti-oxidant, reducing blood lipid, the physiological function such as hypotensive, in fields such as food, cosmetics, medical treatment, chemical industry, have huge development and application to be worth.
Matrimony vine (Lycium barbarum L.) is the Solanaceae Lycium, and the fruit of plant has the effect that nourishing liver and kidney benefiting shrewd head etc. strengthens the human body resistance, is the famous and precious traditional Chinese medicine of China.
Chinese wolfberry fruit is nutritious, includes LBP-X (LBP), flavones, betaine (betaine), 18 seed amino acids, multivitamin (carrotene is maximum), calcium, phosphorus, iron, potassium, organic germanium and growth stimulant.Modern pharmacological research proof matrimony vine reduces blood pressure, and reduces the effects such as cholesterol level.Prevent and treat fatty liver, promote liver cell new life's effect; Malignant tumor patient is improved to the effect of immunologic function.Particularly the polysaccharase immunologic function can be equal to anti-AIDS choice drug AZT, causes the very big concern of international medical community.It also has tonifying kidney and benefiting sperm, moistening lung clearing liver, nourishing blood for improving eyesight, supporing yang improving eyesight, control that void is calmed the nerves, reducing blood lipid blood sugar, anti arteriosclerosis, anticancer, strengthen immunity and the anti-ageing function of waiting for a long time, be the good medicine for the treatment of various diseases, " the angle fruit " that the senior nutrition that is again nourishing, body-building, promotes longevity is generally acknowledge in the world anti-ageing, antitumor, prevent and cure diseases.
This research adopts the high-tech zymolysis technique to obtain the enzyme-hydrolysis albumen powder, be modulated into nutritious, as to strengthen immunity of organisms and applicable adult's taste enzymolysis ovalbumin compound nutrient oral liquor after adding fruit of Chinese wolfberry leaching liquor, for a new way has been opened up in the development and utilization of ovalbumin.
[summary of the invention]
The objective of the invention is to adopt enzyme-hydrolysis albumen and fruit of Chinese wolfberry leaching liquor composite, and a kind of nutrition oral administration that strengthens body immunity obtained.
For achieving the above object, design a kind of enzymolysis ovalbumin compound nutrition oral administration, it is characterized in that adopting following raw material composite and obtain, enzyme-hydrolysis albumen is placed in the reaction vessel with heater, injected water, water is 99.5:0.5 with the ratio of the weight of solids, heating, and heating-up temperature is 30-35 ℃, ratio according to 5:100 adds fruit of Chinese wolfberry leaching liquor to be stirred, mixing time is 1.2-2 hour, after naturally cooling, filter and gets final product to obtain finished product.
Described fruit of Chinese wolfberry leaching liquor adopts following method preparation and obtains: choosing is without disease and pest, without going mouldy high-quality fruit of Chinese wolfberry raw material, after cleaning up with mobile clear water, by matrimony vine and water quality, than 1:10, add 90 ℃ of deionized waters, after soaking 30min, making beating rapidly, material after making beating is lixiviate 30min under 90 ℃ of conditions, with 150 order filter-cloth filterings, filter residue is merged to filtrate twice with same method secondary lixiviate simultaneously, be cooled to 60 ℃, through macroreticular resin decolouring 2.5h, be cooled to room temperature, preserve standby.
The present invention compared with the existing technology, can supplement the nutrient of needed by human, suppresses cytopathy, strengthens body immunity; The active cell activity, remove harmful free radical; Repair the cell of pathology of human body, improve metabolism; Promote the synthetic and regulation and control of human body protein, enzyme and ferment; Tonifying kidney and benefiting sperm, moistening lung clearing liver, nourishing blood for improving eyesight, reduce blood pressure, and reduces cholesterol level, prevents that artery sclerosis and Promote immunity function from improving resistance against diseases anti-senility etc.
[specific embodiment]
Below to further description of the present invention
One, raw material is prepared
1, fruit of Chinese wolfberry lixiviate
Select without disease and pest, without going mouldy high-quality fruit of Chinese wolfberry raw material, wash away silt impurity with mobile clear water, by matrimony vine and water quality, than 1:10, add 90 ℃ of deionized waters, after soaking 30min, making beating rapidly, material after making beating is lixiviate 30min under 90 ℃ of conditions, with 150 order filter-cloth filterings, filter residue is merged to filtrate twice with same method secondary lixiviate simultaneously, be cooled to 60 ℃, through macroreticular resin decolouring 2.5h, be cooled to room temperature, preserve standby.
2, above-mentioned wolfberry can adopt following method to detect
1) assay method of LBP-X
Accurately take matrimony vine 80mg, adding 80% ethanol 50ml puts in conical flask, ultrasonic processing 30min, filter, filter residue 80% ethanol washing 3 times, after filter residue put into to former conical flask together with filter paper add water 60ml, ultrasonic processing 30min, filter, in filtrate dislocation 100ml measuring bottle, constant volume in measuring bottle is put in 2-3 transfer of water washing conical flask, gets 2ml and puts in the 25ml colorimetric cylinder as sample solution.Drying under reduced pressure to the aqueous solution (1mg/ml) of the DEXTROSE ANHYDROUS of constant weight of take is contrast solution, and precision measures 0.2,0.4,0.6,0.8,1.0ml puts in the 25ml colorimetric cylinder is contrast solution preparation calibration curve.Add 5% phenol solution 1ml in the 25ml colorimetric cylinder, shake up, add rapidly sulfuric acid 5ml, shake up, place 10min, put in 40 ℃ of water-baths and be incubated 15min, be cooled to room temperature after taking-up, take corresponding reagent as blank.Wavelength place at 490nm measures absorbance.
2) assay method of general flavone
Precision takes matrimony vine 0.2g, put in the 25ml measuring bottle, adding water 5ml dissolves, then add 65% ethanol constant volume, ultrasonic 30min, filter, precision measures in 2ml to 25ml measuring bottle, 30% ethanolic solution (0.2mg/ml) of rutin of take is contrast solution, precision measures 1, 2, 3, 4, 5ml puts in the 25ml measuring bottle and prepares calibration curve, add successively 2ml water in the 25ml of sample solution and contrast solution measuring bottle, 6m130% ethanol, the 1m15% sodium nitrite solution, after standing 6min, add again the 1m110% aluminum nitrate solution, standing 6min again, add the 10ml sodium hydroxide test solution, 30% ethanol constant volume, standing 15min.Take corresponding reagent as blank.Measure its absorbance under the 500nm wavelength.
2, enzyme-hydrolysis albumen
By market purchasing, obtain
Two, composite
Enzyme-hydrolysis albumen is placed in the reaction vessel with heater, injected water, and water is 99.5:0.5 with the ratio of the weight of solids, heating, heating-up temperature is 30-35 ℃, according to the ratio of 5:100, adds fruit of Chinese wolfberry leaching liquor to be stirred, and mixing time is 1.2-2 hour.After naturally cooling, filter and get final product to obtain finished product.
Three, case
One female patient, 75 years old, AD was COPD concurrent infection, III degree malnutrition.Looking into liver function while being admitted to hospital shows: total protein 45g/L, albumin 20g/L, while being admitted to hospital every day only can take food minute quantity gruel, thin rice gruel and vegetable soup.Through the positive anti-infective treatment that waits, give 2/time of enzyme-hydrolysis albumen nutrition oral administrations every day, 3 times/d, after 20d, liver function is shown total protein 61g/L, albumin 31g/L, original edema, pulmonary infection recovery from illness, be discharged from hospitals upon recovery substantially.
Select 40 routine liver cirrhosis patients, the male sex's 20 examples wherein, women's 20 examples; Year mean age (49.2 ± 17.3).All patients all make a definite diagnosis through inspections such as liver function test, B ultrasonic and gastroscopes.40 routine patients are divided into 2 groups at random, every group of 20 examples.One group is taken white enzyme-hydrolysis albumen nutrition oral administration is experimental group, and one group is taken distilled water is control group.Instructions of taking is every day 3 times, each 10mL, and be 24 weeks the course for the treatment of.Inactive diuretics during oral enzyme-hydrolysis albumen nutrition oral administration, do not transfuse blood and quiet human albumin, to eliminate the impact on curative effect.Experimental group produce effects 10 examples, 8 examples that take a turn for the better, invalid 2 examples; Control group is respectively 2,2,16 examples.
Claims (2)
1. an enzymolysis ovalbumin compound nutrition oral administration, it is characterized in that adopting that following raw material is composite obtains: enzyme-hydrolysis albumen is placed in the reaction vessel with heater, injected water, water is 99.5:0.5 with the ratio of the weight of solids, heating, heating-up temperature is 30-40 ℃, according to the ratio of 5:100, adds fruit of Chinese wolfberry leaching liquor to be stirred, and mixing time is 1.2-2 hour.After naturally cooling, filter and get final product to obtain finished product.
2. the fruit of Chinese wolfberry leaching liquor in enzymolysis ovalbumin compound nutrition oral administration as claimed in claim 1, select without disease and pest, without going mouldy high-quality fruit of Chinese wolfberry raw material, after cleaning up with mobile clear water, add 90 ℃ of deionized waters by matrimony vine and water quality than 1:10, after soaking 30min, making beating rapidly, material after making beating is lixiviate 30min under 90 ℃ of conditions, with 150 order filter-cloth filterings, filter residue is merged to filtrate twice with same method secondary lixiviate simultaneously, be cooled to 60 ℃, through macroreticular resin decolouring 2.5h, be cooled to room temperature, preserve standby.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| CN113230389A (en) * | 2021-04-29 | 2021-08-10 | 意润健康产业（广州）有限公司 | Application of composition comprising enzymolysis ovalbumin micromolecule active short peptide in preparation of medicines and foods for improving hypoalbuminemia |
| US11160299B2 (en) | 2019-07-11 | 2021-11-02 | Clara Foods Co. | Protein compositions and consumable products thereof |
| CN113616771A (en) * | 2020-05-09 | 2021-11-09 | 吉林省拓华生物科技有限公司 | Lyophilized powder of active protein extracted from placenta Cervi, placenta Cervi oral liquid and their preparation methods |
| CN114081110A (en) * | 2021-11-04 | 2022-02-25 | 杭州佰倍优生物科技有限公司 | Albumin enzymolysis beverage |
| US11279748B2 (en) | 2014-11-11 | 2022-03-22 | Clara Foods Co. | Recombinant animal-free food compositions and methods of making them |
| CN115336700A (en) * | 2022-08-22 | 2022-11-15 | 宁夏华宝枸杞产业有限公司 | Decolorized deacidified clear wolfberry juice and preparation method thereof |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US11279748B2 (en) | 2014-11-11 | 2022-03-22 | Clara Foods Co. | Recombinant animal-free food compositions and methods of making them |
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| US11649445B2 (en) | 2019-08-07 | 2023-05-16 | Clara Foods Co. | Compositions comprising digestive enzymes |
| CN113616771A (en) * | 2020-05-09 | 2021-11-09 | 吉林省拓华生物科技有限公司 | Lyophilized powder of active protein extracted from placenta Cervi, placenta Cervi oral liquid and their preparation methods |
| CN113230389A (en) * | 2021-04-29 | 2021-08-10 | 意润健康产业（广州）有限公司 | Application of composition comprising enzymolysis ovalbumin micromolecule active short peptide in preparation of medicines and foods for improving hypoalbuminemia |
| CN114081110A (en) * | 2021-11-04 | 2022-02-25 | 杭州佰倍优生物科技有限公司 | Albumin enzymolysis beverage |
| CN115336700A (en) * | 2022-08-22 | 2022-11-15 | 宁夏华宝枸杞产业有限公司 | Decolorized deacidified clear wolfberry juice and preparation method thereof |
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Application publication date: 20131218 |